Method for producing acrylamide using film technique microbiological transformation

A microbial conversion and acrylamide technology, applied in fermentation and other directions, can solve the problems of high content of biological impurities in acrylamide products, complex equipment maintenance, high energy consumption, etc., to improve reaction efficiency and cell utilization, and simplify production equipment and processes , the effect of simplifying the ion exchange step

Inactive Publication Date: 2004-03-17
TSINGHUA UNIV +1
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Problems solved by technology

The main problems in the current technology are: the process of cell immobilization is relatively complicated, which also increases equipment investment and production costs; the enzyme activity of cells after immobilization is greatly reduced, which affects the utilization of enzyme activity; It will break, produce organic and inorganic impurities, and affect the quality of the reaction solution; therefore, the hydration reaction solution needs to be refined in multiple steps to meet the quality requirements, which makes the refining process heavier, etc.
This process needs to use a high-speed centrifuge, which requires a large investment in equipment, complicated equipment maintenance, high energy consumption, and the content of biological impurities in the obtained acrylamide product is also relatively high.

Method used

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  • Method for producing acrylamide using film technique microbiological transformation
  • Method for producing acrylamide using film technique microbiological transformation

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Experimental program
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Embodiment 1

[0011] (1) Bacteria are cultured in a 3m3 fermenter at 28°C. The main components of the medium are: glucose 20g / L; yeast extract powder 5g / L; urea 7.5g / L; sodium glutamate 1g / L ;K 2 HPO 4 0.5g / L; KH 2 PO 4 0.5g / L; MgSO 4 ·7H 2 O0.5g / L; inducer 60ppm. The pH value was adjusted to 7.5, and after 56 hours of cultivation, a total of about 1.2 tons of bacteria suspension was obtained, the wet bacteria content was 120g / L, and the enzyme activity reached 2669U / mL.

[0012] (2) Use a pump to send the bacterium supersuspension to the thalline washing tank, and feed cooling brine into the cooling jacket of the washing tank to control the temperature of the bacteria solution at 4-10° C. during the washing process.

[0013] (3) Start the circulation pump, the speed is 2900rpm, and the flow rate is 12.5m 3 / h, the bacterial suspension is filtered and concentrated through the hollow fiber microfiltration membrane, and the filtrate is discharged into the sewage treatment syst...

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Abstract

The microbial conversion process of producing acrylamide by applying membrane technology includes the following steps: microbial thallus culture, preparation of heavy thallus suspension, acrylonitilehydration to produce acrylamide with free thallus as biocatalyst, and separating reaction obtained acrylamide hydrate liquid. The present invention features the microfiltering film purification and washing of thallus in fermented liquid to prepare heavy thallus suspension and the ultrafiltering film separation of acrylamide hydrate liquid from biological impurity. The production process of acrylamide has high production efficiency, high thallus utilization, low biological impurity content in hydrate product, and high quality and purity of acrylamide product.

Description

technical field [0001] The invention relates to the application method of membrane technology in the production of acrylamide by microbial conversion, mainly including the application of microfiltration membrane in the process of free cell washing, the application of ultrafiltration membrane in the process of free cell catalyzed hydration reaction and the process composed of these two parts The whole production process of acrylamide microbial transformation. It belongs to the field of biochemical technology. Background technique [0002] The microbial conversion method is a new generation of industrial production of acrylamide. The technology used is mainly based on alginate-immobilized cells as biocatalysts and in batch reaction mode. Its technological process is divided into 8 parts: fermentation, granulation, hydration, refining, concentration, crystallization, centrifugation and drying. In its hydration section, the immobilized particles are separated from the hydratio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02
Inventor 沈忠耀孙旭东于慧敏史悦端木勉刘正光沈瑞华顾宏军薛建菲
Owner TSINGHUA UNIV
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