1894 results about "Thallus" patented technology

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The present invention relates to the industrial application of ocean fission chytrid OUC88. The ocean fission chytrid OUC88 has capacity of generating rich docosahexaenoic acid (DHA), and has the preservation number of CGMCC No. 1240. The ocean fission chytrid OUC88 is new strain obtain with fission chytrid SR21 as originating strain and through chemical induction mutation and physical induction mutation. Through optimizing culture condition and replenishing carbon source in later culture stage, the present invention has dry cell weight reaching 25 g/L and DHA product up to 8.27 g/L. The thallus may be used as the additive for bread, mild product and other food products, especially baby's milk powder and can promote body's growth. The present invention has simple thallus fermenting process, high bioavailability, high yield of destination product and low cost.

The invention relates to bacillus amyloliquefaciens NCPSJ7 and further relates to an application of the strain in treating plant diseases, diseases before and after harvesting fruits and vegetables as well as in preventing food-borne pathogenic bacteria and putrefying bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC) on March 22, 2013, wherein the preservation number is CCTCC NO: M2013098 and the strain is named as bacillus amyloliquefaciens NCPSJ7. The thallus and fermentation liquor of the strain disclosed by the invention has the effects of treating plant diseases caused by plant pathogenic fungus including antagonistic peach root rotten disease, fusarium wilt of cucumber, botrytis cinerea, jujube anthracnose, pear black spot, pear blue mould, apple brown rot, apple altermaria leaf spot, watermelon fusarium wilt and the like, as well as diseases after harvesting fruits and vegetables and food-borne pathogenic bacteria and putrefying bacteria including antagonistic staphylococcus aureus, salmonella paratyphi A, vibrio parahaemolyticus, yeast and the like; moreover, the bacillus amyloliquefaciens is broad in spectrum and antibacterial and great in potential in developing novel, efficient and natural biological control and biological preservative and fresh-keeping preparation.

The invention discloses a preparing method of new type lipopeptide biosurfactant surfactin and appliance, which comprises the following steps: utilizing bacillus subtilis strain E8 (conserved in Chinese microbe conservative council common microbe center, the keeping number at 1107) to proceed microbe liquid ferment prepare surfactant surfactin; proceeding carbon source, nitrogen source and inorganic salt optimize for the needed liquid culture medium; getting fermentation liquor; centrifuging to remove thallus; acidifying supernatant; extracting rough extract; further-extracting through dissolvent; acidifying; collecting deposition; centrifuging; vacuum-drying; getting pre-refined product; freeze-drying through drainage pillar and FPLC extraction; getting product with high purity; making the output of acidifying extraction at 24-56 h reach 5-13g/L. The dominant fermentate molecular weight of high-yield strain E8 can reach 1022Da and CMC can reach 1 mu M, which is the lowest of reported surfactin CMC value.

The invention discloses a Pseudomonas stutzeri JSD-008 and degradation for organophosphorus pesticide, which is preserved in the 'Chinese germ management committee center (CGMCC)' with preservation number at CGMCC No.1738, wherein the strain is separated from the soil at pollution discharge pore of pesticide processing plant polluted by organophosphorus pesticide; the thallus and ferment liquid with the thallus can be degradation agent of organophosphorus pesticide to degrade the chlorpyrifos into trichlopyridinenol and methyl parathion into nitrophenol; fitting for rapid in-situ rehabilitation polluted by field soil pesticide.

The invention relates to a method for producing L-lactic acid. In the method, crop straw and Rhizopus oryzae serve as raw materials. The method particularly comprises the following operation steps: A. preparing straw saccharified liquid; B. adding auxiliary material to inoculate Rhizopus oryzae for fermentation; C repeatedly fermenting 25 batches; D. acidizing fermented clear liquid; E. ultrafiltration; F. nanofilteration; G. reverse osmosis; H. ion exchange; I. concentrating in vacuum to obtain the L-lactic acid product with the mass percentage concentration of 85%. The invention takes the straw hydrolysis saccharified liquid as the main raw material to prepare L-lactic acid, which obviously lowers the production cost of L-lactic acid; Rhizopus oryzae is adopted for once inoculation proliferation, thallus can be reused for several times to produce L-lactic acid, thus shortening L-lactic acid fermentation period, and obviously improving the fermentation strength of L-lactic acid; an advanced membrane separation and ion exchange integration technology is adopted to improve the lactic acid product quality; and the invention has simple process and strong continuity.

The invention discloses a preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth. A strain is pseudomonas chlororaphis HT66CCTCC No:M2013467. A main metabolite of the strain comprises phenazine-1-phenazine and phenazine-1 formamide. The strain is inoculated into a microorganism culture medium and is fermented to obtain a thallus with high concentration and the metabolite, and can be mixed with materials like powder active carbon, bentonite, swell soil, kaolin, diatomite, zeolite, calcium carbonate and the like to prepare a microbial agent; the quantity of viable organisms in a finished product is 1-8.5*10<9>cfu/mL. The tablet laboratory verifies that the pseudomonas chlororaphis has good control effect of pathogenic bacteria of rice sheath blight diseases, cotton fusarium wilt, gaeumannomyces graminis and watermelon fusarium wilt, and also has a good promotion function in the growth of a part of plants.

The invention discloses a method for extracting and refining docosahexaenoic acid from schizochytrium, which is characterized by comprising the following steps: carrying out flocculation processing ofschizochytrium ATCC 20888 fermentation liquid, then carrying out solid-liquid separation, mechanically crushing cells after alkali cell wall is broken, and extracting crushed thallus by adopting organic solvents to obtain DHA rough oil; processing the DHA rough oil by adopting the steps of degumming, alkali refining, decoloring and deodorizing to obtain DHA essential oil. The invention is simplein operation, can reduce the extraction cost of the DHA oil, only adopts the organic solvents during the operating process, has no residues finally, improves the quality of the DHA oil after being refined, has the DHA content in the extract about 28-51 percent and is suitable for large-scale production of DHA.

The invention discloses bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding diseases. The collection number of the Bacillus amyloliquefaciens H47 is CCTCC NO: M2013283. A biological agent which resists the peach bleeding diseases is prepared by carrying out liquid fermentation on the strain can be applied to peach trees; the liquid fermentation process comprises the following steps of: slant strain activation, seed culture and liquid deep fermentation culture. The strain disclosed by the invention and thallus-free spore-free fermentation liquor can both generate a higher antagonistic effect on the pathogen botryosphaeria of the peach bleeding diseases; the biological agent disclosed by the invention achieves an outstanding preventing and controlling effect on peach tree bleeding diseases; compared with the traditional chemical agent, the biological agent disclosed by the invention has the advantages of greenness, safety, environment friendliness, and the like and has wide application prospect on the aspect of biological prevention and control of the bleeding diseases.

The present invention discloses one kind of biological bactericide for fruits and vegetable and its preparation process. The biological bactericide consists of 10<8>-10<11> CFU/ml concentration Tallman yeast cell suspension 100-1000 ml, and sodium ethyl hydroxyl benzoate 0.6-1.2 g or sodium butyl hydroxyl benzoate 0.6-1.2 g. The preparation process includes the following steps: 1. inoculating the saccharommycete to NYDB culture medium inside a flask, culturing at 26-28 deg.c and 150-200 rpm for 18-30 hr, centrifuging the fermented liquid at 2000-3000 rpm for 5-10 min to collect thallus, twice washing with abacterial water, counting with a blood counting chamber and regulating the concentration of the suspension to 10<8>-10<11> CFU/ml; and 2. mixing the suspension, sodium hydroxyl benzoate and water in the ratio of 100 to 1 to 105-100 to 1 to 106 via stirring.

The invention discloses a method for synthesizing ethyl caproate under the catalysis of yeast display lipase. The method comprises two steps, namely the production of full cellular zymin and the synthesis of ethyl caproate. The method comprises the following: a step of cloning the lipase gene into the Pichia yeast surface display vector pKFS to construct the Pichia yeast surface display expression vector pKFS-lipase which is transformed into the host bacteria of Pichia yeast through linearization, a yeast genetic engineering bacteria capable of displaying active pheron on the surface of the Pichia yeast being obtained through screening, and the engineering bacteria being fermented in a rocking bottle to obtain a thallus which is used to make the full cellular zymin after 24 hours of vacuum freeze drying; and then a step of adopting caproic acid and alcohol as raw material which is esterified at the action of the biocatalyst of yeast full cellular zymin with the lipase displayed on the surface to obtain the ethyl caproate product. The method is capable of collecting thalli centrifugally for reuse after the reaction with short reaction time, high yield and good operational stability, thereby greatly reducing production costs.

The invention provides a high-density culture method of saccharomyces cerevisiae and a pH regulation and control method of the saccharomyces cerevisiae. The high-density culture method comprises the following steps: performing first-stage and second-stage seed enlarging cultivation; centrifugating, washing and enriching seeds; putting the seeds into a fermentation tank filled with water for fermental cultivation, and feeding molasses and a yeast extract powder solution during fermentation. The pH regulation and control method comprises the following steps: monitoring a physiological state of thallus in the fermentation tank on line and in real time, and regulating and controlling the speed of feeding molasses and yeast extract powder according to a carbon dioxide release speed, an oxygen intake speed and changes of a respiratory quotient, so as to regulate and control the pH value in the fermentation tank, wherein no inorganic salt, acid, alkali and trace elements are required to be added in the whole fermentation, regulation and control process; at the end of fermentation, performing separation and washing, and cooling saccharomyces cerevisiae thallus. The method disclosed by theinvention is easy to operate and low in cost; the prepared saccharomyces cerevisiae is edible and has high protein content; a fermentation raw material is high in conversion rate; no acid and alkali are added, so that the operation safety is improved.

The invention provides schizochytrium LX0809 (marine fungus), which has a high docosahexaenoic acid (DHA) production capability. The schizochytrium LX0809 of the invention was separated from the corroded soil collected from the red beach in Zhaoquanhe Town, Dawa County, Liaoning Province and was preserved in China General Microbiological Culture Collection Center with a preservation number CGMCC No.3535 on December 23th, 2009. When the schizochytrium LX0809 is used for fermenting supplementary food, the dry cell weight of the fermentation liquor can reach 72.5 g/L, and the DHA content can reach 15.3g/L. LX 0809 thallus cells and extracts thereof can be used as food additives for edible oil, dairy products, grain products and the like and can be used as a feed additive for aquaculture and livestock culture and the like. Saturated triglycercide in the thallus cells can be used to produce biodiesel through catalytic transesterification.

The invention relates to the technical field of marine environment management, in particular to an offshore oil degrading microbial inoculum and a preparation method thereof. The invention discloses an offshore oil degrading microbial inoculum, which comprises a petroleum degrading flora and a biological fixation carrier, and is characterized in that the microbial inoculum is prepared by attaching the petroleum degrading flora on the floating degradable biological fixation carrier. According to the invention, the biological fixation carrier takes an expanding degradable material as a core, and an outer layer is coated with a degradable composite membrane with positive electricity; the biological fixation carrier has floating property through the expanding degradable material; the degradable composite membrane with positive electricity and a thallus with negative electricity can attract mutually, so that the bacterium are firmly attached on the carrier, and the composite membrane is a porous loose structure, so that the degrading flora can be attached on the wall of the porous structure of the composite membrane; in comparison with the simple petroleum degrading flora, the degrading efficiency of the degrading microbial inoculum is remarkably improved. The offshore oil degrading microbial inoculum, provided by the invention, is expected to be used for bio-remediation of marine oil pollution.

The invention discloses a lactobacillus leavening agent, a preparation method thereof and a special bacterial strain. The preservation serial number of lactobacillus is CCTCC M208151. An active constituent of the lactobacillus leavening agent is the lactobacillus. The invention also discloses a method for preparing the lactobacillus leavening agent, which comprises the following steps: firstly, leavening and culturing lactobacillus plantarum SC79 CCTCC M208151 in SC79 optimal culture medium; and secondly, collecting thallus in the step 1 and then adding a protective agent to the thallus to obtain the leavening agent. The lactobacillus leavening agent has small dosage, quick acid production speed through mixing the lactobacillus leavening agent and a harvestless exopolysacchatide yoghourt bacterial strain to prepare yoghourt, better relative viscosity, elasticity, denseness and adhesiveness than that of the conventional yogurt and small syneresis sensibility of formed sticky yogurt colloids, larger water holding capacity than that the conventional yogurt and hard whey separation.

The invention relates to a method used microorganism enzyme resolution DL-pantoic acid lactone to produce D-pantoic acid. It uses the D-pantoic acid lactone hydrolase strain of the fusarium, gibberella, aspergillus, penicillium, rhizopus, gliocladium, aureobasidium to ferment and culture, uses wet thallus as coarse enzyme, DL-pantoic acid lactone as substrate to produce D-pantoic acid. L- pantoic acid lactone can be reclaimed. The DL-pantoic acid lactone gained by racemization reaction can newly be used to do resolution.

The invention provides a preparation method of 3'-sialic acid lactose, belonging to the technical field of biological engineering. The preparation method comprises the following steps of: taking colibacillus CCTCC (colibacillus China center for type culture collection) NO: M208088 as a starting strain, adding a fermentation medium into a fermentation tank, fermenting for 2-6h, then adding a fed batch culture medium, and adding lactose in a flowing way at the mid-later period of the fermentation; and centrifuging the fermentation liquid to obtain liquid supernatant 1 and a thallus, diluting the centrifuged thallus by adding water and splitting in a heating way, recentrifuging to obtain liquid supernatant 2, merging the liquid supernatant 1 and the liquid supernatant 2, and absorbing, desorbing and vacuum drying by ion exchange resin to obtain the 3'-sialic acid lactose. A mass of the 3'-sialic acid lactose can be prepared by the CCTCC NO: M208088. The 3'-sialic acid lactose can change the current situation that the sialyloligosaccharide component is less in exogenous adding substances in conventional baby milk, and can provide the guarantee to add more efficient sialic acid trophic factors into the baby milk.

The invention provides a preparation method of a spore preparation of bacillus coagulans. The preparation method of the spore preparation of bacillus coagulans comprises the following steps of: (a) slope thallus activation; (b) seeding tank cultivation of the bacillus coagulans; and (c) solid fermentation of the bacillus coagulans: placing the culture medium in the step (b) in a solid fermentation medium based on the weight ratio of 1%-10%, and fermenting for 48-72hours in a semi-closed manner. By using a semi-closed solid fermentation method, materials are in the semi-closed state in the process of fermentation and contact with a small amount of air, the bacillus coagulans can be propagated greatly by fully utilizing residual oxygen in the growing period, oxygen is greatly reduced in the later growing period, spore can grow rapidly, and more L-lactic acid can be metabolized, so that the problem that produced acid is insufficient in the fermentation process is solved, the growth of infectious microbe is inhibited through oxygen consumption, and the phenomenon of solid fermentation pollution is avoided.

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is characterized by being prepared from the following materials in percentage by weight: 40.0-50.0 % of rape seed cakes, 10.0-20.0% of crops straws, 1.5-3.0% of mycothallus, 0.5-1.0% of bacterium thallus and 35.0-40.0% of water. By using the solid fermentation method twice, the invention organically combines the fungi (Trichoderma harzianum and Aspergillus aculeatus for biological control effect) and the bacteria (Bacillus subtillis and Bacillus megatherium for growth-promoting effect), and realizes a simple preparation method. The compound microbial fertilizer has the advantages of environmental condition resistance, high count of active viable bacteria, less influence by the environment after application, slow release of thallus and long-term effective colonization at rhizosphere of plants, promotes plant growth and simultaneously has a certain biological control effect on soil-borne diseases.

The invention relates to the technical field of biological catalysis and particularly relates to a recombinant expression vector, genetically engineered bacteria containing the recombinant expression vector and a method for producing 1,5-pentanediamine by virtue of whole cell conversion of the recombinant expression vector. The recombinant expression vector comprises a nucleotide sequence for expressing cadB and a nucleotide sequence for expressing cadA of lysine decarboxylase, wherein two nucleotide sequences are respectively regulated, controlled and transcribed by one independent promoter. The recombinant expression vector is characterized in that a periplasm secreting signal peptide coding sequence is fused to a terminal 5' of the nucleotide sequence for expressing cadB. The activity of the thallus of the genetically engineered bacteria is high; preliminary study finds that by carrying out whole cell conversion on a 343g/l substrate, the content of 1,5-pentanediamine reaches 221 g/l and is far superior to that in the report of the prior art.

The invention relates to a micro-capsulated suspended microbial seed coating agent and a preparation method thereof. The micro-capsulated suspended microbial seed coating agent comprises a functional bacteria microcapsule and auxiliaries, wherein the functional bacteria microcapsule is prepared from wall materials such as sodium alginate and gelatin by virtue of an endogenous emulsion process, the grain size of the microcapsule is 20-60 mu m, the content of functional bacteria is not lower than 10<9>cfu/g, and the embedding rate is over 80%. Functional microbes are selected from two or more of pseudomonas, bacillus subtilis, bacillus amyloliquefaciens, enterobacter cloacae, raoultella planticola and bacillus atrophaeus. The auxiliaries comprise one or more of a film-forming agent, a dispersant, a tackifier, an anti-freezing agent and a coloring agent. The prepared functional bacteria microcapsule is uniformly mixed with the auxiliaries to obtain a finished product. The product has the advantages of good film-forming performances, thallus persistence, high survivability, good slow-controlled release, strong application stability, environment friendliness, convenience in use, and the like, can be used for promoting seed germination and seedling growth of cottons, soya beans, wheat and potatoes, and the like, controlling the diseases at the seedling stage, improving stress resistance, and the like.

The method relates to a method for preparing collagen peptide with a fish skin, comprising the steps of: washing, draining and cutting the fish skin into pieces, soaking the fish skin pieces in 75% ethanol for 15 min, then taking them out and washing by soaking them in sterile water for 3-5 times and then draining; putting the fish skin pieces into a fermenter so as to keep the skin pieces with a concentration of 4%-8%, inoculating strains with an amount of 1%-5% and a cell age of 14h-18h for fermentation at a temperature of 30-37 DEG C for 48-56h, with 30%-40% of dissolved oxygen, and after fermentation filtering the fermentation liquor to remove thalluses, then conducting enzyme destruction, vacuum concentration and spray drying to the fermentation liquor, thus obtaining collagen peptide. Compared with present methods for preparing collagen peptide with a fish skin, the method of the invention prepares collagen peptide directly through the fermentation method. Thus the preparation process of collagen peptide is simplified, and the production cost is reduced, without environmental pollution. Therefore, the method of the invention has good application prospect.

The invention discloses a method for quickly producing carotenoid by utilizing microaerobic fermentation of rhodobacter sphaeroides. The method comprises the following particular steps that the activated rhodobacter sphaeroides is inoculated to an MMS (methylmercuric sulfate) culture medium; aerobic culture is performed until OD600 (optical density 600) is 0.6-0.8; a culture condition is adjusted to be microaerobic; the carotenoid is induced to be quickly and greatly synthesized; and then extraction and purification are performed. The time for producing the carotenoid by the method is short and only 36h; the yield of thallus is up to 6g/L; the yield and the productivity of the carotenoid are 8.287mg/L and 1911mug/g respectively; the carotenoid has good capacity for removing DPPH (1,1-diphenyl-2-picryl-hydrazyl) radicals; and IC50 (half maximal inhibitory concentration) is about 8mug/mL.