69 results about "Thallus" patented technology

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of bacillus velezensis fermentation liquor in prevention and control of citrus red mites. A bacillus velezensis XZT106 strain is subjected to fermentation treatment to obtain fermentation liquor without thalli, the fermentation liquor contains effective components including epothilone A and silthiopham, experiments prove that the death rate of red spiders treated by a stock solution of the fermentation liquor is 100%, the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7%, and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 500 times and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7% respectively. In addition, it is further verified that the fermentation liquor of the bacillus velezensis XZT106 thallus does not generate the problems of drug resistance and environmental pollution in the process of preventing and treating the red spiders.

The invention belongs to the technical field of biomedical treatment and biological fermentation, discloses a metagen composition for effectively inhibiting oral dental caries and application thereof, and aims to solve the problems of great side effect, weak effect and unknown mechanism of the existing dental caries prevention and treatment means. The composition is prepared by mixing a metagen 1 which is prepared by fermenting lactobacillus salivarius Postbio-YY with a metagen 2 which is prepared by fermenting lactococcus lactis Postbio-F3 and lactobacillus paracasei Postbio-P6 step by step. In the preparation process, the active ingredients are reserved by accurately controlling the fermentation pH, time and pasteurization parameters. The composition can be prepared into multiple dosage forms such as toothpaste and the like, can remarkably inhibit the growth of streptococcus mutans and destroy the thallus structure, inhibits biofilm and exopolysaccharide synthesis by down-regulating fimI, wcaJ and wcae genes, is safe and free of side effects, and provides an efficient scheme for decayed tooth prevention and treatment.

The invention relates to a method for inhibiting rumen gas production by using a genetically modified laver filament as a ruminant feed additive and a method for inhibiting methane production by using the genetically modified laver filament as the ruminant feed additive. A porphyra yezoensis bromine peroxidase gene is cloned, an overexpression vector of the gene is constructed, porphyra yezoensis thallus is used as a receptor for genetic transformation, and a porphyra algal strain of which the key enzyme expression quantity is remarkably improved is obtained through screening. The method comprises the following steps: inducing thalli to generate protonema, crushing the protonema by using a crusher, carrying out ventilated large-scale culture in a seawater culture medium added with PES by using a column bioreactor to obtain a large number of protonema materials, collecting the protonema materials, crushing the protonema materials by using the crusher, and drying at low temperature. The obtained laver protonema powder is put into a vacuum bag for low-temperature preservation in dark light, and can be applied to preparation of ruminant feed as a dairy cow feed additive. The filamentous can accumulate halogenated compounds such as bromidogen and the like, can inhibit the activity of a key enzyme generated by methane in rumen, and further inhibits the generation of methane.

The invention relates to the technical field of agricultural product management and control, in particular to a photovoltaic-edible mushroom collaborative intelligent agricultural industry fusion management and control method and system, and provides the following scheme: generating an input data set for energy-production collaborative analysis by obtaining multi-source monitoring data of a photovoltaic power generation and edible mushroom production unit; the physiological critical period of the edible mushrooms is recognized based on manifold embedding and topological matching algorithms, an anti-fact manifold matching model under energy constraint is constructed, and verifiable judgment of physiological state changes is achieved; dynamically evaluating the stability and energy consumption efficiency of the thalli in combination with a stage perception and energy generation mapping empirical model; and a hierarchical multi-objective optimization algorithm is adopted to generate a regulation and control scheme considering the energy utilization efficiency and the thallus stability, and the regulation and control scheme is output to an energy management end. According to the method, the collaborative optimization of the thallus growth stability and the system energy efficiency can be kept in the fluctuating energy environment, and the method is suitable for photovoltaic agriculture, intelligent edible mushroom factories and new energy-biological coupling type agriculture scenes.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation method for increasing the content of pyocyanin in pseudomonas aeruginosa fermentation liquor. The invention provides a novel pseudomonas aeruginosa fermentation method. The multiplication capacity and metabolic activity of thalli are regulated and enhanced through a microgravity environment. According to the method, single colonies of pseudomonas aeruginosa are cultured through streak isolation, activated and then inoculated to a fermentation medium, fermentation is performed under the microgravity condition of 0.001 g, the microgravity environment fermentation remarkably optimizes thallus growth, the pyocyanin yield is increased, and the biofilm forming ability is enhanced.

The utility model discloses an edible fungus incubator networking alarm device, which relates to the technical field of edible fungus cultivation, comprises a box body, and is characterized in that a box door is arranged on one side of the box body, a sealing gasket is arranged on one side, close to the box body, of the box door, and three mounting blocks are arranged on each of two sides of the inner wall of the box body. And draining plates are arranged among the three groups of mounting blocks. The sealing effect between the box body and the box door can be improved through the arranged sealing gasket, external dust and impurities are prevented from entering the box body to influence thallus embryos, when the box body needs to be atomized and humidified, some small water drops in the culture groove are gathered together, water can fall into the draining groove through the draining holes, and therefore the thallus embryos are prevented from being damaged. Therefore, some water can be prevented from being deposited in the culture tank for a long time to influence thalli in the culture tank, the quality of produced edible mushrooms is ensured, the culture plate can be taken out by pulling the handle, the water collected in the draining tank can be conveniently recycled, and waste of water resources is avoided.

The invention discloses a cordyceps sinensis culture control method and system, and core culture targets are set in generations by identifying the subculture generations G1, G2 and G3 of cordyceps sinensis strains: the G1 generation activates a cordycepin and adenosine synthesis pathway, the G2 generation maximizes the total nucleoside content and antioxidant activity, and the G3 generation improves the biomass and delays degradation. The system monitors environmental parameters such as temperature and humidity, illumination, gas concentration and the like, and thallus physiological indexes such as biomass, metabolism precursor concentration, active substance content and the like in real time, and adopts algorithms such as fuzzy PID, NSGA-II multi-objective optimization, model prediction control and the like to dynamically adjust culture conditions such as nutrition supply, dissolved oxygen, illumination period and the like. The method provided by the invention solves the problem that traditional static regulation neglects intergenerational difference of strains, realizes subgeneration refined targeted regulation, remarkably improves the yield and quality of target products, prolongs the service cycle of the strains, and is suitable for industrial bionic cultivation of cordyceps sinensis.

The invention discloses a transcription factor HbNAC47 related to saline-alkaline tolerance, and a coding gene and application thereof. The amino acid sequence of the transcription factor HbNAC47 related to the saline-alkaline tolerance character is as shown in SEQ ID No. 1. The nucleotide sequence of the coding gene of the transcription factor HbNAC47 related to the saline-alkaline tolerance character is as shown in SEQ ID No. 2. The transcription factor HbNAC47 or the coding gene thereof is applied to cultivation of saline-alkaline tolerant transgenic plants. The invention further provides a recombinant expression vector, an expression cassette or a recombinant thallus of the coding gene of the transcription factor HbNAC47 related to the saline-alkaline tolerance character and application of the recombinant expression vector, the expression cassette or the recombinant thallus in cultivation of saline-alkaline tolerant transgenic plants. By overexpressing the barley grass HbNAC47 gene, the expression of salt-alkali tolerant related genes is regulated, so that a salt-alkali tolerant plant is obtained, and the barley grass HbNAC47 gene has great production and application values.

The invention discloses a non-sterilization type mildew-proof and antibacterial material capable of dynamically responding to thallus proliferation as well as a preparation method and application of the non-sterilization type mildew-proof and antibacterial material. The material comprises polyvinyl alcohol, carboxymethyl cellulose or a salt thereof and metal ions, and the polyvinyl alcohol, the carboxymethyl cellulose or the salt thereof are bridged through the metal ions; the metal ions comprise Li < + >. The material disclosed by the invention takes Na < + > released by thallus proliferation as a signal medium, releases Li < + > as required through a coordination-dissociation process, and further induces thallus cells to actively ingest Li < + > through metabolic deception of occupying a potassium ion channel by utilizing a hydration radius of Li < + > similar to K < + > according to the metabolic demand of sodium excretion and potassium ingestion in the thallus proliferation process; on one hand, bacterial cell replication lacks essential nutrient substance K < + >, and finally gradually declines; on the other hand, protein space conformation is changed and inactivated by capturing water molecules of intracellular protein, and the two aspects have a synergistic effect, so that the efficient mildew-proof and antibacterial effects of Li < + > at low concentration can be realized, and the effect is lasting.

To provide a technique for suppressing maturation of seaweed.SOLUTION: The foregoing problem is solved by a seaweed culturing method comprising a step of culturing seaweed under supply of microbubbles and a step of collecting the seaweed.SELECTED DRAWING: None

The invention discloses a screening method and application of a floating moss endogenous constitutive promoter, and belongs to the technical field of gene engineering. A floating moss endogenous constitutive promoter is screened out through experiments such as screening verification of candidate constitutive promoters, a plant expression vector of which a reporter gene started by the promoter is positioned in a cell nucleus is constructed, and floating moss is transformed to obtain a transgenic plant. The fluorescence intensity of the reporter gene driven by the promoter is higher than that of other common constitutive promoters, such as CaMV35S and the like, and meanwhile, when fluorescence expression of transgenic plants is observed, it is found that the reporter gene can be stably expressed in thalli, scales, various different types of cells of the thalli and the scales and the like. The invention provides an efficient and universal core tool for floating moss gene function research, genetic manipulation, biotechnology application and the like, and has a certain application prospect.

The invention relates to a method for preparing pentamethylene diamine through continuous conversion and a production strain thereof. The invention belongs to the technical field of microorganisms, and particularly relates to a genetically engineered bacterium capable of continuously producing pentamethylene diamine and application of the genetically engineered bacterium. According to the pentamethylene diamine biotransformation genetically engineered bacterium disclosed by the invention, kdsD, lpxL, pagP, lpxP, eptA, lpxM, OmpF and OmpC are deleted, so that the thallus can be self-settled after the transformation of the strain is completed, convenience is provided for subsequent continuous transformation and extraction and purification of pentamethylene diamine, the accumulation of pentamethylene diamine reaches a relatively high level after multiple continuous transformation processes, and the cost is saved. The invention also provides a thallus sedimentation mode, which is characterized in that 10-20 mM of Ca < 2 + >, Mg < 2 + > and 5-40 g / L of LMat01 are added on the basis of the genetically engineered bacterium disclosed by the invention, so that thalli can be completely settled within 15 minutes, and can still be completely settled within 15 minutes after multiple times of transformation. According to the invention, by establishing a thallus sedimentation process, a strain required by synthesis of pentamethylene diamine and an efficient preparation method thereof are constructed, so that a continuous preparation process of pentamethylene diamine is realized.

The invention discloses a hydrogel and probiotic compounded microbial inoculum composition and a vegetable planting matrix, a three-dimensional gel carrier is used for embedding a microbial inoculum, the carrier integrates mechanical properties and thallus protection and can maintain flora activity for a long time, bacillus thuringiensis has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression, and the microbial inoculum composition has the advantages that the microbial inoculum composition has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression; the microbial agent composition can survive for a long time in a low-oxygen environment of a carrier embedding environment, and is cooperated with bacillus cereus, azotobacter chroococcum and bacillus megatherium to promote the growth of plants such as vegetables and the like and directionally kill pathogenic microorganisms such as root-knot nematode. Corresponding pests can be killed in a green and targeted manner, the growth of plants is promoted, and the yield is increased.

The invention discloses a stable genetic transformation method of floating moss. The genetic transformation method comprises the following steps: (1) crushing thallus tissues of the floating moss, (2) pre-culturing explants, (3) infecting and co-culturing, (4) screening and culturing for the first time, (5) screening and culturing for the second time, (6) enlarging and culturing, and (7) verifying transformation. According to the genetic transformation method, a thallus of floating moss is taken as a starting explant, an agrobacterium-mediated hygromycin and kanamycin screening transformation system of the floating moss is established for the first time, the blank of a floating moss genetic transformation system is filled, and experimental results show that after multi-generation culture, a transformed plant can still stably express a target gene. The method has the advantages of high transformation efficiency and simplicity in operation, and can be used for subsequent genetic transformation research of unknown floating moss genes. The method has a wide application prospect.

The invention relates to the technical field of biological fermentation, and provides a process method for efficiently synthesizing gamma-aminobutyric acid, which comprises the following steps: constructing an optimized seed activation culture medium and a high-density thallus culture system, promoting the expression of glutamate decarboxylase by adopting a gradient temperature induction strategy, and realizing the production of GABA by utilizing a whole-cell catalysis technology. In the catalytic reaction stage, a reaction system composed of engineering bacteria, a glutamic acid substrate, coenzyme and water is constructed, a two-stage substrate adding strategy is adopted, dynamic supplement is carried out by monitoring the concentration change of glutamic acid in real time, and conversion of glutamic acid to GABA is promoted. By applying the method, whole-cell catalytic production with the substrate concentration of 750g / L can be realized, the product conversion rate reaches 99.9% or above, and the thalli can still keep the catalytic activity of 70% or above after being continuously and repeatedly used for 3 batches. The whole process is simple and convenient to operate, a catalytic system is simple in composition, introduction of impurities is effectively avoided, and the process difficulty of subsequent separation and purification is reduced.

The invention belongs to the technical field of biological medicine, and particularly relates to a probiotic preparation for repairing chemical liver injury and a preparation method. The preparation method of the probiotic preparation comprises the following steps: inoculating a fermentation culture medium with activated lactobacillus plantarum liquid, fermenting to obtain a fermentation mixture, centrifuging the fermentation mixture, collecting thallus precipitate, and resuspending by using a freeze-drying protective agent to obtain resuspension liquid; freeze-dried powder obtained by carrying out vacuum freeze-drying on the resuspension is the probiotic preparation; by taking the probiotic preparation disclosed by the invention, the generation of inflammatory factors related to liver injury can be effectively reduced, the liver injury of mice can be relieved by improving oxidative stress, and the probiotic preparation has prevention and treatment effects on chemical liver injury.

The invention provides a biotin fermentation process optimization control method, system and device and a storage medium, and relates to the technical field of biotin ferment.The optimization control method comprises the steps that in the biotin fermentation process, basic data of a fermentation control system is obtained; the basic data comprises temperature data in the fermentation tank, monitoring data reflecting the state of fermentation liquor and dissolved oxygen concentration data in the fermentation liquor; based on the basic data, corresponding control processes are executed respectively; the control process comprises a temperature control process, a pH value dynamic prediction control process and a dissolved oxygen multi-parameter cascade control process. According to the method, the stability of the production process is integrally enhanced, the automation level and the product consistency are improved, the dependence on artificial experience is reduced through closed-loop intelligent cooperative operation of the three processes, a highly stable environment is created for thallus growth and product synthesis, and the production efficiency is improved. Therefore, the final yield, purity and reproducibility among production batches of biotin B5 are directly improved, and comprehensive energy consumption and material consumption are reduced.

The invention discloses bacillus velezensis and application thereof, and relates to the technical field of biological control. The bacillus is a strain GZSF-g1B, and the preservation number of the bacillus is CGMCC (China General Microbiological Culture Collection Center) No.36194. According to the GZSF-g1B thallus suspension disclosed by the invention, indoor control effect determination is carried out on millet anthracnose by using the thallus suspension, and the control effect is 84.83%.

The invention relates to the technical field of material chemical industry, in particular to a method and a system for extracting natural pigment for coating based on microbial fermentation, which comprises the following steps: acquiring a plurality of initial strains, acquiring a genetic recombination strain group, preparing single-cell suspension from the genetic recombination strains, performing fluorescence detection on the single-cell suspension, and confirming an optimal mutant strain. The method comprises the following steps: obtaining a mutant strain pigment crude extract, confirming a maximum absorption wavelength, culturing an optimal mutant strain, respectively carrying out absorbance determination on each mutant strain experimental group to obtain an absorbance value set, confirming an optimal fermentation temperature, carrying out large-scale fermentation to obtain a high-secretion fermentation liquid, carrying out wall breaking treatment to obtain a wall-broken thallus suspension, and carrying out freeze drying to obtain the high-secretion thallus suspension. The method comprises the following steps: preparing an intracellular pigment solution, carrying out microencapsulation on the intracellular pigment solution to obtain pigment microspheres, extracting a verification pigment extracting solution from the pigment microspheres, and obtaining the natural pigment by utilizing the verification pigment extracting solution. The problems of low extraction efficiency, high energy consumption and environmental pollution during extraction of the natural pigment for the coating can be solved.

The invention discloses a tissue culture method of floating moss, which comprises the following steps: disinfecting thalli of the floating moss as explants by 3-7% NaClO for 4-6 minutes to obtain sterile materials of the floating moss, and comparing the growth speeds and growth conditions of the floating moss on four different culture mediums. The invention discloses an optimal disinfection method and an optimal growth and rapid propagation culture medium of floating moss, establishes a tissue culture and rapid propagation system of floating moss, and lays a foundation for subsequent establishment of a genetic transformation system of floating moss and cell biology research.

The embodiment of the utility model discloses an integrated fermentation device capable of simultaneously fermenting three thalli, the fermentation device comprises a tank body and a motor, the outside of the tank body is of a cuboid structure, and the inside of the tank body is divided into a first fermentation area, a second fermentation area and a third fermentation area along the length direction of the cuboid; a rotating shaft penetrating through the first fermentation area, the second fermentation area and the third fermentation area is arranged in the tank body; stirring blades are arranged on the rotating shaft; and the motor is fixedly connected with the input end of the rotating shaft. The integrated fermentation tank disclosed by the utility model is provided with three fermentation areas, so that three strains can be fermented at the same time, and the thallus culture efficiency is improved; besides, each fermentation area is provided with a heat exchanger, so that the temperature of each fermentation area can be independently adjusted to adapt to culture of different strains; in addition, the stirring blades are arranged to be spiral, so that fermentation liquor in the three fermentation areas can be effectively stirred at the same time.

The invention relates to arthrobacter sp., an arthrobacter sp. Microbial inoculum and application. The classification name of the pseudoarthrobacter sp. Is Pseudarthrobacter sp., and the preservation number of the pseudoarthrobacter sp. Is GDMCC 1.4159. The pseudoarthrobacter sp. Has the advantages that the pseudoarthrobacter sp. Can be used for preparing the bacillus subtilis; the pseudoarthrobacter inoculant contains a culture medium and a thallus, and the thallus contains pseudoarthrobacter of which the preservation number is GDMCC 1.4159. The arthrobacter sp. Obtained by the invention can degrade atrazine and organophosphorus, reduce the pollution of atrazine and organophosphorus in pesticides to the environment, and effectively reduce the harm of the pesticides to the environment and human beings; in addition, the pseudoarthrobacter disclosed by the invention not only is relatively high in degradation efficiency, but also is free from secondary pollution, and has a relatively good application value.

An outlet of a fermentation tank is connected with an inlet of a centrifugal machine, a clear liquid outlet of the centrifugal machine is connected with a first inlet of a batching tank, an outlet of the batching tank is connected with a cold material inlet of a first heat exchanger, and a cold material outlet of the first heat exchanger is connected with a material inlet of a second heat exchanger; a material outlet of the second heat exchanger is connected with a material inlet of the maintaining heat exchanger, a material outlet of the maintaining heat exchanger is connected with a hot material inlet of the first heat exchanger, and a hot material outlet of the first heat exchanger is connected with a material inlet of the third heat exchanger. A material outlet of the third heat exchanger is respectively connected with inlet ends of a first branch pipeline and a second branch pipeline through a main pipeline, an outlet end of the first branch pipeline is connected with an inlet of the fermentation tank, and an outlet end of the second branch pipeline is connected with a second inlet of the batching tank. The system improves the reuse water proportion, cancels part of equipment, saves the cost, reduces the risk of hot water leakage, and reduces the operation difficulty and microbiological contamination risk.

The invention discloses a method for promoting growth of nitrite oxidizing bacteria, and belongs to the technical field of culture of nitrifying microorganisms in the field of environmental microorganisms. According to the method, an adsorption medium can be provided for growth of bacteria, the growth speed of the nitrite oxidizing bacteria is increased, under the condition that cationic polyacrylamide and closed cell perlite are used as culture medium additives, the growth speed of the nitrite oxidizing bacteria is remarkably increased compared with that under the condition of an original culture medium, and the production period is shortened. The method is simple to operate, low in cost and capable of theoretically reducing cost and increasing benefits, on one hand, the culture time is shortened, on the other hand, the cell density of unit volume in the culture medium is averaged due to the fact that the closed-cell perlite is low in density, thalli are adsorbed on holes of the closed-cell perlite and are easily dispersed along with flowing of culture medium liquid, and then the competitive pressure between the thalli is reduced; the thallus culture density and the thallus obtaining amount of the system are improved, and the production cost is greatly reduced compared with that of a conventional large-scale aeration stirring culture method.

The invention belongs to the field of cell engineering seedling culture of algae, and particularly relates to a cell engineering seedling culture method of gracilaria lemaneiformis. The gracilaria lemaneiformis frond is constructed into a miniaturized form, and high-density intensive culture is carried out by utilizing a photobioreactor. According to the method, the microminiaturized asparagus frond obtained by a cell engineering means can be subjected to vegetative propagation, efficient propagation is carried out by utilizing the algae photobioreactor, and the microminiaturized asparagus frond is further cultured into seedlings for artificial cultivation.

The invention discloses a subcellular localization method suitable for floating moss, and belongs to the technical field of mutation or genetic engineering and DNA or RNA and vectors related to the genetic engineering. According to the method disclosed by the invention, the tender thallus of the floating moss is used as a material, and a related protein positioning gene is transferred into a floating moss species through a floating moss stable genetic transformation system. A series of subcellular localization genes are obtained through cloning in a floating moss genome for the first time, the genes can assist GFP fusion protein to enter different subcellular organelles, and a marker line (localization strain) is obtained through stable transformation of a floating moss material. The method can be used for verifying a subcellular localization result of a candidate floating moss target gene, and is a simple, convenient and stable floating moss subcellular localization method. The method has a wide application prospect.

The invention discloses an intestinal beneficial viable bacterium culture and extraction device in the technical field of thallus culture, the intestinal beneficial viable bacterium culture and extraction device comprises a main drying box and a crushing box, the crushing box is fixedly mounted on one side of the main drying box, a drying assembly is arranged in the main drying box, the drying assembly comprises a drawing assembly, and the drawing assembly is composed of two clamping plates; sealing baffles are fixedly mounted at the two ends of the two groups of clamping plates which are arranged in parallel up and down; the two groups of sealing baffles are clamped with the outer wall of the main drying box through clamping mechanisms; the drying equipment is composed of drying units symmetrically installed at the upper end and the lower end of the main drying box, the drying units can be attached to the surfaces of the clamping plates, the drawing assembly is used for bearing the original materials, and the drying equipment is used for drying the materials on the drawing assembly; according to the beneficial bacterium drying device, the beneficial bacterium drying storage rack can be quickly cleaned, so that original materials on the storage rack are heated more uniformly and comprehensively.

The invention relates to bacillus velezensis, a microbial agent and application. The bacillus velezensis is classified and named as bacillus velezensis, and the preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) No.27206. The bacillus velezensis has the advantages that the bacillus velezensis is named as bacillus velezensis; the invention further relates to a microbial agent, the microbial agent contains a culture medium and a thallus, and the thallus contains the bacillus velezensis with the preservation number of CGMCC No.27206. The bacillus velezensis and the microbial agent provided by the invention have relatively good fusarium oxysporum resistance, can reduce the morbidity and disease index of alfalfa root rot, and are relatively high in control efficiency.

The invention relates to the technical field of laver fertilization, and particularly discloses a spraying fertilization method for increasing yield by utilizing laver in a dry and dew period, which comprises the following specific steps: determining a fertilization opportunity; preparing a nutrient solution: respectively preparing a phosphorus nutritive salt nutrient solution and a humic acid diluent; wetting pretreatment before application; spraying and applying by an unmanned aerial vehicle: after the wetting pretreatment is completed, immediately and uniformly spraying the prepared phosphorus nutritive salt nutrient solution and humic acid diluent on the laver thalli through a spraying device carried by the unmanned aerial vehicle. According to the method, through synergistic application, the fertilization efficiency is greatly improved, labor saving and large-scale operation are achieved, raw materials used in the method are easy to obtain, equipment is mature, the method has good engineering feasibility and generalization performance, dilution and waste of a nutrient solution are effectively avoided, the utilization rate of the fertilizer is remarkably increased, and the method is suitable for large-scale production. According to the method, humic acid is applied at a precise time, so that growth of the laver is remarkably promoted, and the yield is increased.

The invention provides application of a sarcodia montagneana extract in preparation of a composition for inhibiting Japanese encephalitis virus. Wherein the sarcodia montagneana extract is extracted from a sarcodia montagneana frond material or a sarcodia montagneana frond, the sarcodia montagneana extract contains a component for inhibiting the Japanese encephalitis virus, the sarcodia montagneana extract can be applied to resisting infection of the Japanese encephalitis virus and inhibiting the activity of the Japanese encephalitis virus, and the sarcodia montagneana extract does not have cytotoxicity.