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42 results about "Thallus" patented technology

Thallus (plural: thalli), from Latinized Greek θαλλός (thallos), meaning "a green shoot" or "twig", is the undifferentiated vegetative tissue of some organisms in diverse groups such as algae, fungi, some liverworts, lichens, and the Myxogastria. Many of these organisms were previously known as the thallophytes, a polyphyletic group of distantly related organisms. An organism or structure resembling a thallus is called thalloid, thallodal, thalliform, thalline, or thallose.

Application of bacillus velezensis fermentation liquor in prevention and treatment of citrus red mites

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of bacillus velezensis fermentation liquor in prevention and control of citrus red mites. A bacillus velezensis XZT106 strain is subjected to fermentation treatment to obtain fermentation liquor without thalli, the fermentation liquor contains effective components including epothilone A and silthiopham, experiments prove that the death rate of red spiders treated by a stock solution of the fermentation liquor is 100%, the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7%, and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 500 times and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7% respectively. In addition, it is further verified that the fermentation liquor of the bacillus velezensis XZT106 thallus does not generate the problems of drug resistance and environmental pollution in the process of preventing and treating the red spiders.
Owner:HEBEI GUANWEIDA BIOTECHNOLOGY CO LTD +1

Photovoltaic-edible mushroom collaborative intelligent agricultural industry fusion management and control method and system

The invention relates to the technical field of agricultural product management and control, in particular to a photovoltaic-edible mushroom collaborative intelligent agricultural industry fusion management and control method and system, and provides the following scheme: generating an input data set for energy-production collaborative analysis by obtaining multi-source monitoring data of a photovoltaic power generation and edible mushroom production unit; the physiological critical period of the edible mushrooms is recognized based on manifold embedding and topological matching algorithms, an anti-fact manifold matching model under energy constraint is constructed, and verifiable judgment of physiological state changes is achieved; dynamically evaluating the stability and energy consumption efficiency of the thalli in combination with a stage perception and energy generation mapping empirical model; and a hierarchical multi-objective optimization algorithm is adopted to generate a regulation and control scheme considering the energy utilization efficiency and the thallus stability, and the regulation and control scheme is output to an energy management end. According to the method, the collaborative optimization of the thallus growth stability and the system energy efficiency can be kept in the fluctuating energy environment, and the method is suitable for photovoltaic agriculture, intelligent edible mushroom factories and new energy-biological coupling type agriculture scenes.
Owner:SHANGHAI HENGZE FUHUI INTELLIGENT TECHNOLOGY CO LTD

Networking alarm device for edible mushroom culture box

The utility model discloses an edible fungus incubator networking alarm device, which relates to the technical field of edible fungus cultivation, comprises a box body, and is characterized in that a box door is arranged on one side of the box body, a sealing gasket is arranged on one side, close to the box body, of the box door, and three mounting blocks are arranged on each of two sides of the inner wall of the box body. And draining plates are arranged among the three groups of mounting blocks. The sealing effect between the box body and the box door can be improved through the arranged sealing gasket, external dust and impurities are prevented from entering the box body to influence thallus embryos, when the box body needs to be atomized and humidified, some small water drops in the culture groove are gathered together, water can fall into the draining groove through the draining holes, and therefore the thallus embryos are prevented from being damaged. Therefore, some water can be prevented from being deposited in the culture tank for a long time to influence thalli in the culture tank, the quality of produced edible mushrooms is ensured, the culture plate can be taken out by pulling the handle, the water collected in the draining tank can be conveniently recycled, and waste of water resources is avoided.
Owner:ZHENJIANG GUMANYUAN ECOLOGICAL AGRI CO LTD

Cordyceps sinensis culture control method and system

PendingCN121343773AFungiMicrobiological testing/measurementAdenosine synthesisNutrition
The invention discloses a cordyceps sinensis culture control method and system, and core culture targets are set in generations by identifying the subculture generations G1, G2 and G3 of cordyceps sinensis strains: the G1 generation activates a cordycepin and adenosine synthesis pathway, the G2 generation maximizes the total nucleoside content and antioxidant activity, and the G3 generation improves the biomass and delays degradation. The system monitors environmental parameters such as temperature and humidity, illumination, gas concentration and the like, and thallus physiological indexes such as biomass, metabolism precursor concentration, active substance content and the like in real time, and adopts algorithms such as fuzzy PID, NSGA-II multi-objective optimization, model prediction control and the like to dynamically adjust culture conditions such as nutrition supply, dissolved oxygen, illumination period and the like. The method provided by the invention solves the problem that traditional static regulation neglects intergenerational difference of strains, realizes subgeneration refined targeted regulation, remarkably improves the yield and quality of target products, prolongs the service cycle of the strains, and is suitable for industrial bionic cultivation of cordyceps sinensis.
Owner:YICAO BIOTECHNOLOGY (HEBEI) CO LTD

Transcription factor HbNAC47 related to saline-alkaline tolerance, and coding gene and application thereof

The invention discloses a transcription factor HbNAC47 related to saline-alkaline tolerance, and a coding gene and application thereof. The amino acid sequence of the transcription factor HbNAC47 related to the saline-alkaline tolerance character is as shown in SEQ ID No. 1. The nucleotide sequence of the coding gene of the transcription factor HbNAC47 related to the saline-alkaline tolerance character is as shown in SEQ ID No. 2. The transcription factor HbNAC47 or the coding gene thereof is applied to cultivation of saline-alkaline tolerant transgenic plants. The invention further provides a recombinant expression vector, an expression cassette or a recombinant thallus of the coding gene of the transcription factor HbNAC47 related to the saline-alkaline tolerance character and application of the recombinant expression vector, the expression cassette or the recombinant thallus in cultivation of saline-alkaline tolerant transgenic plants. By overexpressing the barley grass HbNAC47 gene, the expression of salt-alkali tolerant related genes is regulated, so that a salt-alkali tolerant plant is obtained, and the barley grass HbNAC47 gene has great production and application values.
Owner:NORTHEAST FORESTRY UNIV

Non-sterilization type mildew-proof antibacterial material capable of dynamically responding to thallus proliferation as well as preparation method and application of non-sterilization type mildew-proof antibacterial material

The invention discloses a non-sterilization type mildew-proof and antibacterial material capable of dynamically responding to thallus proliferation as well as a preparation method and application of the non-sterilization type mildew-proof and antibacterial material. The material comprises polyvinyl alcohol, carboxymethyl cellulose or a salt thereof and metal ions, and the polyvinyl alcohol, the carboxymethyl cellulose or the salt thereof are bridged through the metal ions; the metal ions comprise Li < + >. The material disclosed by the invention takes Na < + > released by thallus proliferation as a signal medium, releases Li < + > as required through a coordination-dissociation process, and further induces thallus cells to actively ingest Li < + > through metabolic deception of occupying a potassium ion channel by utilizing a hydration radius of Li < + > similar to K < + > according to the metabolic demand of sodium excretion and potassium ingestion in the thallus proliferation process; on one hand, bacterial cell replication lacks essential nutrient substance K < + >, and finally gradually declines; on the other hand, protein space conformation is changed and inactivated by capturing water molecules of intracellular protein, and the two aspects have a synergistic effect, so that the efficient mildew-proof and antibacterial effects of Li < + > at low concentration can be realized, and the effect is lasting.
Owner:DONGHUA UNIV

Screening method and application of floating moss endogenous constitutive promoter

PendingCN121574978ABryophytesVectorsBiotechnologyGenetic engineering
The invention discloses a screening method and application of a floating moss endogenous constitutive promoter, and belongs to the technical field of gene engineering. A floating moss endogenous constitutive promoter is screened out through experiments such as screening verification of candidate constitutive promoters, a plant expression vector of which a reporter gene started by the promoter is positioned in a cell nucleus is constructed, and floating moss is transformed to obtain a transgenic plant. The fluorescence intensity of the reporter gene driven by the promoter is higher than that of other common constitutive promoters, such as CaMV35S and the like, and meanwhile, when fluorescence expression of transgenic plants is observed, it is found that the reporter gene can be stably expressed in thalli, scales, various different types of cells of the thalli and the scales and the like. The invention provides an efficient and universal core tool for floating moss gene function research, genetic manipulation, biotechnology application and the like, and has a certain application prospect.
Owner:SHANGHAI HUATAI BIOTECHNOLOGY CO LTD

Stable genetic transformation method of floating moss

PendingCN121472318ABryophytesFermentationBiotechnologyKanamycin
The invention discloses a stable genetic transformation method of floating moss. The genetic transformation method comprises the following steps: (1) crushing thallus tissues of the floating moss, (2) pre-culturing explants, (3) infecting and co-culturing, (4) screening and culturing for the first time, (5) screening and culturing for the second time, (6) enlarging and culturing, and (7) verifying transformation. According to the genetic transformation method, a thallus of floating moss is taken as a starting explant, an agrobacterium-mediated hygromycin and kanamycin screening transformation system of the floating moss is established for the first time, the blank of a floating moss genetic transformation system is filled, and experimental results show that after multi-generation culture, a transformed plant can still stably express a target gene. The method has the advantages of high transformation efficiency and simplicity in operation, and can be used for subsequent genetic transformation research of unknown floating moss genes. The method has a wide application prospect.
Owner:SHANGHAI HUATAI BIOTECHNOLOGY CO LTD

Biotin fermentation process optimization control method, system and equipment and storage medium

The invention provides a biotin fermentation process optimization control method, system and device and a storage medium, and relates to the technical field of biotin ferment.The optimization control method comprises the steps that in the biotin fermentation process, basic data of a fermentation control system is obtained; the basic data comprises temperature data in the fermentation tank, monitoring data reflecting the state of fermentation liquor and dissolved oxygen concentration data in the fermentation liquor; based on the basic data, corresponding control processes are executed respectively; the control process comprises a temperature control process, a pH value dynamic prediction control process and a dissolved oxygen multi-parameter cascade control process. According to the method, the stability of the production process is integrally enhanced, the automation level and the product consistency are improved, the dependence on artificial experience is reduced through closed-loop intelligent cooperative operation of the three processes, a highly stable environment is created for thallus growth and product synthesis, and the production efficiency is improved. Therefore, the final yield, purity and reproducibility among production batches of biotin B5 are directly improved, and comprehensive energy consumption and material consumption are reduced.
Owner:SUPCON TECH CO LTD

Bacillus velezensis and application thereof

The invention discloses bacillus velezensis and application thereof, and relates to the technical field of biological control. The bacillus is a strain GZSF-g1B, and the preservation number of the bacillus is CGMCC (China General Microbiological Culture Collection Center) No.36194. According to the GZSF-g1B thallus suspension disclosed by the invention, indoor control effect determination is carried out on millet anthracnose by using the thallus suspension, and the control effect is 84.83%.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Tissue culture method of floating moss

The invention discloses a tissue culture method of floating moss, which comprises the following steps: disinfecting thalli of the floating moss as explants by 3-7% NaClO for 4-6 minutes to obtain sterile materials of the floating moss, and comparing the growth speeds and growth conditions of the floating moss on four different culture mediums. The invention discloses an optimal disinfection method and an optimal growth and rapid propagation culture medium of floating moss, establishes a tissue culture and rapid propagation system of floating moss, and lays a foundation for subsequent establishment of a genetic transformation system of floating moss and cell biology research.
Owner:SHANGHAI HUATAI BIOTECHNOLOGY CO LTD

Arthrobacter sp., arthrobacter sp. Microbial inoculum and application of arthrobacter sp.

The invention relates to arthrobacter sp., an arthrobacter sp. Microbial inoculum and application. The classification name of the pseudoarthrobacter sp. Is Pseudarthrobacter sp., and the preservation number of the pseudoarthrobacter sp. Is GDMCC 1.4159. The pseudoarthrobacter sp. Has the advantages that the pseudoarthrobacter sp. Can be used for preparing the bacillus subtilis; the pseudoarthrobacter inoculant contains a culture medium and a thallus, and the thallus contains pseudoarthrobacter of which the preservation number is GDMCC 1.4159. The arthrobacter sp. Obtained by the invention can degrade atrazine and organophosphorus, reduce the pollution of atrazine and organophosphorus in pesticides to the environment, and effectively reduce the harm of the pesticides to the environment and human beings; in addition, the pseudoarthrobacter disclosed by the invention not only is relatively high in degradation efficiency, but also is free from secondary pollution, and has a relatively good application value.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI

Method for promoting growth of nitrite oxidizing bacteria

The invention discloses a method for promoting growth of nitrite oxidizing bacteria, and belongs to the technical field of culture of nitrifying microorganisms in the field of environmental microorganisms. According to the method, an adsorption medium can be provided for growth of bacteria, the growth speed of the nitrite oxidizing bacteria is increased, under the condition that cationic polyacrylamide and closed cell perlite are used as culture medium additives, the growth speed of the nitrite oxidizing bacteria is remarkably increased compared with that under the condition of an original culture medium, and the production period is shortened. The method is simple to operate, low in cost and capable of theoretically reducing cost and increasing benefits, on one hand, the culture time is shortened, on the other hand, the cell density of unit volume in the culture medium is averaged due to the fact that the closed-cell perlite is low in density, thalli are adsorbed on holes of the closed-cell perlite and are easily dispersed along with flowing of culture medium liquid, and then the competitive pressure between the thalli is reduced; the thallus culture density and the thallus obtaining amount of the system are improved, and the production cost is greatly reduced compared with that of a conventional large-scale aeration stirring culture method.
Owner:SOUTH CHINA UNIV OF TECH

Gracilaria lemaneiformis cell engineering seedling raising method

The invention belongs to the field of cell engineering seedling culture of algae, and particularly relates to a cell engineering seedling culture method of gracilaria lemaneiformis. The gracilaria lemaneiformis frond is constructed into a miniaturized form, and high-density intensive culture is carried out by utilizing a photobioreactor. According to the method, the microminiaturized asparagus frond obtained by a cell engineering means can be subjected to vegetative propagation, efficient propagation is carried out by utilizing the algae photobioreactor, and the microminiaturized asparagus frond is further cultured into seedlings for artificial cultivation.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Subcellular localization method suitable for floating moss

The invention discloses a subcellular localization method suitable for floating moss, and belongs to the technical field of mutation or genetic engineering and DNA or RNA and vectors related to the genetic engineering. According to the method disclosed by the invention, the tender thallus of the floating moss is used as a material, and a related protein positioning gene is transferred into a floating moss species through a floating moss stable genetic transformation system. A series of subcellular localization genes are obtained through cloning in a floating moss genome for the first time, the genes can assist GFP fusion protein to enter different subcellular organelles, and a marker line (localization strain) is obtained through stable transformation of a floating moss material. The method can be used for verifying a subcellular localization result of a candidate floating moss target gene, and is a simple, convenient and stable floating moss subcellular localization method. The method has a wide application prospect.
Owner:SHANGHAI HUATAI BIOTECHNOLOGY CO LTD

Intestinal beneficial viable bacterium culture and extraction device

The invention discloses an intestinal beneficial viable bacterium culture and extraction device in the technical field of thallus culture, the intestinal beneficial viable bacterium culture and extraction device comprises a main drying box and a crushing box, the crushing box is fixedly mounted on one side of the main drying box, a drying assembly is arranged in the main drying box, the drying assembly comprises a drawing assembly, and the drawing assembly is composed of two clamping plates; sealing baffles are fixedly mounted at the two ends of the two groups of clamping plates which are arranged in parallel up and down; the two groups of sealing baffles are clamped with the outer wall of the main drying box through clamping mechanisms; the drying equipment is composed of drying units symmetrically installed at the upper end and the lower end of the main drying box, the drying units can be attached to the surfaces of the clamping plates, the drawing assembly is used for bearing the original materials, and the drying equipment is used for drying the materials on the drawing assembly; according to the beneficial bacterium drying device, the beneficial bacterium drying storage rack can be quickly cleaned, so that original materials on the storage rack are heated more uniformly and comprehensively.
Owner:SICHUAN LIXINMING SMART MEDICAL TECHNOLOGY CO LTD

Bacillus velezensis, microbial agent and application

The invention relates to bacillus velezensis, a microbial agent and application. The bacillus velezensis is classified and named as bacillus velezensis, and the preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) No.27206. The bacillus velezensis has the advantages that the bacillus velezensis is named as bacillus velezensis; the invention further relates to a microbial agent, the microbial agent contains a culture medium and a thallus, and the thallus contains the bacillus velezensis with the preservation number of CGMCC No.27206. The bacillus velezensis and the microbial agent provided by the invention have relatively good fusarium oxysporum resistance, can reduce the morbidity and disease index of alfalfa root rot, and are relatively high in control efficiency.
Owner:INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS

Spraying and fertilizing method for increasing yield by using laver in dry and dew period

ActiveCN121511739AFertilising methodsPorphyraUncrewed vehicle
The invention relates to the technical field of laver fertilization, and particularly discloses a spraying fertilization method for increasing yield by utilizing laver in a dry and dew period, which comprises the following specific steps: determining a fertilization opportunity; preparing a nutrient solution: respectively preparing a phosphorus nutritive salt nutrient solution and a humic acid diluent; wetting pretreatment before application; spraying and applying by an unmanned aerial vehicle: after the wetting pretreatment is completed, immediately and uniformly spraying the prepared phosphorus nutritive salt nutrient solution and humic acid diluent on the laver thalli through a spraying device carried by the unmanned aerial vehicle. According to the method, through synergistic application, the fertilization efficiency is greatly improved, labor saving and large-scale operation are achieved, raw materials used in the method are easy to obtain, equipment is mature, the method has good engineering feasibility and generalization performance, dilution and waste of a nutrient solution are effectively avoided, the utilization rate of the fertilizer is remarkably increased, and the method is suitable for large-scale production. According to the method, humic acid is applied at a precise time, so that growth of the laver is remarkably promoted, and the yield is increased.
Owner:JIANGSU OCEAN UNIV

Preparation method of composite probiotic preparation for removing in-vivo plasticizer

The invention discloses a preparation method of composite probiotics for removing an in-vivo plasticizer. The method comprises the following steps: resuspending a mixed thallus of lactobacillus paracasei, lactobacillus plantarum and bifidobacterium by using a protective solution containing trehalose, mannan oligosaccharide and L-proline; mixing with a functional gel precursor composed of L-aspartic acid, carboxylated beta-cyclodextrin and porous corn starch, and carrying out cross-linking molding in a calcium ion and optional zinc ion system; then performing surface coordination treatment by using a green tea extract and ferric ions, sequentially coating a chitosan-pectin composite layer and an enteric polymer layer, and performing low-temperature drying to obtain the composite probiotic particles. The obtained preparation has high stability in a gastric acid environment, and can realize directional slow release of thalli and efficient adsorption of a plasticizer.
Owner:KEMENG BIOTECHNOLOGY (HANGZHOU) CO LTD

Seed island porphyra-based mutagenesis breeding method and application thereof

PendingCN122397618ABiotechnologySporeling
The application provides a mutagenesis breeding method based on seed island Porphyra and application thereof, and belongs to the technical field of breeding.The mutagenesis breeding method based on seed island Porphyra provided by the application comprises the following steps: selecting immature seed island Porphyra thalli for segmentation to obtain segmented leaves; the segmented leaves are subjected to ARTP mutagenesis, the leaves after mutagenesis are subjected to light-avoiding culture, and then mechanical crushing is performed to 20-40 cell sizes, and then the mutants are screened.The application combines the single spore dissemination technology induced by damage and the ARTP technology, uses the seed island Porphyra thalli as mutagenesis materials for mutagenesis, greatly shortens the mutagenesis period and reduces the workload, has the advantages of high efficiency, high mutation rate and good specificity, and can obtain stable mutants.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA +1

Method for efficient and stable genetic transformation of algal strains by agrobacterium tumefaciens-mediated porphyra yezoensis

The invention provides a method for efficient and stable genetic transformation of an algae strain by agrobacterium tumefaciens-mediated porphyra yezoensis, and belongs to the technical field of plant transgenosis. According to the method, a mixed suspension of porphyra yezoensis thallus fragments, monospores and thallus plantlets is used as a transformation receptor, and an efficient and stable genetic transformation technology is established through the steps of infection solution optimization, gradient screening culture and the like. The technical problems that existing porphyra yezoensis genetic transformation is low in efficiency, poor in repeatability, difficult in transformant screening and the like are effectively solved, the transformation efficiency can reach 30%-40%, the genetic stability of positive plants is close to 100%, a reliable technical platform is provided for gene function verification, character improvement and molecular breeding of porphyra yezoensis, and important scientific research value and application prospects are achieved.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA +1

Application of lactobacillus johnsonii YH1136 inactivated thallus in preparation of products for relieving depression, anxiety and sleep disorders

PendingCN121695183ABacteriaNervous disorderNeurotrophic factorsAnxiety
The invention provides application of lactobacillus johnsonii YH1136 inactivated thalli in preparation of products for relieving depression, anxiety and sleep disorders, and relates to the technical field of microorganisms. The inactivated thallus is obtained by carrying out heat treatment on live thallus of lactobacillus johnsonii YH1136 with the preservation number of CCTCC NO: M 20221116 at 60 to 68 DEG C for 30 to 90 minutes. Animal experiments show that the inactivated thallus can significantly improve depression-like behaviors, anxiety-like behaviors and sleep disorders of CUMS model mice, and the effect of the inactivated thallus is equivalent to that of viable bacteria and positive drug fluoxetine, and is significantly superior to that of 100 DEG C traditional high-temperature inactivated thallus. The action mechanism is related to inhibition of HPA hyperfunction and up-regulation of hippocampal brain-derived neurotrophic factors and prefrontal cortex 5-hydroxytryptamine levels. The inactivated thallus provided by the invention is high in stability, good in safety and suitable for developing related drugs.
Owner:BEIJING GUOYING ZHILIAN BIOENGINEERING TECHNOLOGY CO LTD

Medical and edible homologous fermentation electrical stimulation parameter AI regulation and control method based on signal modulation

The invention provides a method for regulating and controlling an electrical stimulation parameter AI of homologous medicinal and edible fermentation based on signal modulation, and relates to the technical field of homologous medicinal and edible ferment.The method comprises the steps that stage characteristic data, including thallus growth data and metabolite data, of a homologous medicinal and edible fermentation system are collected in real time; inputting the stage feature data into a pre-trained fermentation stage recognition model, and outputting a current fermentation stage; calling a corresponding parameter modulation model based on the current fermentation stage, inputting the stage characteristic data into the parameter modulation model, and generating a target electrical stimulation parameter; and sending the target electrical stimulation parameter to a predetermined electrical stimulation execution module, applying a modulated electrical stimulation signal to the fermentation system, and stopping electrical stimulation modulation until the concentration of the target active component in the fermentation system reaches a preset endpoint threshold. The invention solves the problems that the existing electrical stimulation regulation lacks a signal characteristic optimization mechanism dynamically matched with the fermentation stage, resulting in poor growth and metabolism collaboration of thalli and low yield of active components.
Owner:FUDA LIFE TECH (GUANGZHOU) CO LTD

Feed additive containing cyperus esculentus meal resistant starch and preparation method thereof

The invention discloses a feed additive containing cyperus esculentus meal resistant starch and a preparation method thereof, and relates to the technical field of biology. The feed additive comprises the following components in parts by weight: 90-110 parts of cyperus esculentus meal resistant starch and 5-15 parts of bifidobacterium breve microcapsules, the preparation method of the bifidobacterium breve microcapsule comprises the following steps: carrying out fermentation culture on bifidobacterium breve, and separating to obtain thalli; carrying out embedding and freeze-drying treatment on the thallus and an embedding liquid to obtain a primary microcapsule; preparing the primary microcapsule into calcium alginate gel beads by adopting a dropping method, and then carrying out curing treatment to obtain the bifidobacterium breve microcapsule. The embedding liquid comprises mulberry leaf polysaccharide and algin. The feed additive provided by the invention can improve the growth performance of broiler chickens, improve the intestinal health, improve the meat quality and reduce the emission of ammonia gas and hydrogen sulfide gas in the manure of the broiler chickens.
Owner:HENAN UNIVERSITY OF TECHNOLOGY +3

Method for preparing L-cystine by adopting three-phase horizontal spiral centrifuge

The invention belongs to the field of amino acid preparation, and discloses a method for preparing L-cystine by adopting a three-phase horizontal spiral centrifuge, which comprises the following steps: (1) sequentially carrying out thallus wall breaking and flocculation operation on an L-cystine precipitate-bacterium-salt mixed solution to obtain a multi-phase mixed system; and (2) separating the multi-phase mixed system obtained in the step (1) through a three-phase horizontal spiral centrifuge to respectively obtain L-cystine precipitate, protein heavy liquid and brine clear liquid, and further purifying the L-cystine precipitate to obtain the L-cystine. According to the method, the separation steps are effectively shortened, the separation operation of a ceramic membrane is reduced, and the separation efficiency of L-cystine is improved.
Owner:HEILONGJIANG NHU BIOTECH CO LTD +1

A method for efficiently and stably transforming porphyra yezoensis by agrobacterium

The application provides a method for efficiently and stably transforming algae strains of Porphyra yezoensis by Agrobacterium mediation, and belongs to the technical field of plant transgenesis. The application uses a mixed suspension of leaf thallus fragments, single spores and leaf thallus seedlings of Porphyra yezoensis as a transformation receptor, and establishes an efficient and stable genetic transformation technology through steps such as optimization of an infection solution and gradient screening culture. The application effectively solves the technical problems of low genetic transformation efficiency, poor repeatability and difficulty in screening of transformants of the existing Porphyra yezoensis, and the transformation efficiency can reach 30% to 40%, and the genetic stability of positive plants is close to 100%, thereby providing a reliable technical platform for gene function verification, trait improvement and molecular breeding of Porphyra yezoensis, and having important scientific research value and application prospect.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA +1

Gene for coding omega-transaminase, recombinant expression vector, genetically engineered bacterium, preparation method and application of genetically engineered bacterium, and method for producing vanillylamine

The invention provides a gene for coding omega-transaminase, a recombinant expression vector, a genetically engineered bacterium, a preparation method and application of the genetically engineered bacterium and a method for producing vanillylamine, and particularly belongs to the technical field of biochemical engineering. The invention provides a gene for coding omega-transaminase. The nucleotide sequence of the gene is shown as SEQ ID NO. 1. According to the present invention, the gene is expressed in the genetically engineered bacterium, the obtained thallus is adopted as the whole-cell catalyst, the catalytic activity on vanillin is high, the catalytic efficiency is high, the core catalyst is provided for the biological synthesis of vanillylamine, and the important theoretical value and the important application potential are provided.
Owner:SHIHEZI UNIVERSITY

Composite electrode material for selective lead removal and preparation method and application thereof

The invention belongs to the technical field of environmental functional materials, and discloses a composite electrode material for selective lead removal and a preparation method and application thereof. The preparation method of the composite electrode material for selective lead removal comprises the following steps: culturing and collecting bacillus cereus, sequentially adding CoCl2. 6H2O and NaBH4 into a thallus suspension, reacting, centrifuging, washing, drying and calcining to obtain the Co3O4-x and thallus derived carbon composite electrode material. The composite electrode material is combined with double electric layers and a Faraday storage mechanism, the Pb < 2 + > adsorption capacity is 60-120 mg / g, the adsorption rate is 2.0-4.0 mg / g / min, the performance is remarkably superior to that of a traditional activated carbon electrode, and the key problems that cobalt-based oxide is poor in conductivity, poor in selectivity and insufficient in cycling stability in capacitive deionization application are effectively solved.
Owner:NANHUA UNIV

Fermentation device for preparing bacillus subtilis and fermentation production process

The invention discloses a fermentation device for bacillus subtilis preparation and a fermentation production process, and belongs to the technical field of bacillus subtilis preparation, the fermentation device comprises a fermentation tank, the interior of the fermentation tank is divided into a fermentation cavity and a driving cavity; the stirring mechanism is arranged in the fermentation tank. According to the fermentation device for bacillus subtilis preparation and the fermentation production process, by using the stirring mechanism, three-dimensional mixing is achieved, stirring dead angles are eliminated, and by means of a revolution, autorotation and lifting combined motion mode, stirring blades form a complex three-dimensional flow field in the fermentation tank, so that the stirring effect is improved; the whole fermentation cavity from the tank top to the tank bottom and from the center to the edge can be fully and uniformly mixed, the problem of mixing dead angles existing in a traditional single stirrer is solved, a uniform nutrition and dissolved oxygen environment is provided for thallus growth, meanwhile, the depth and the rotating speed of stirring blades can be intelligently adjusted according to different fermentation stages and dissolved oxygen feedback, and the fermentation efficiency is improved. And high-efficiency mixing and mass transfer are always kept in the whole fermentation period.
Owner:WEIFANG KANGDATE PHARMACEUTICAL CO LTD +2

Preparation method and application of metagen product containing ergothioneine and achyranthes bidentata polysaccharide and capable of improving immunity

The invention discloses a preparation method and application of a metagen product containing ergothioneine and achyranthes bidentata polysaccharides and capable of improving immunity, and relates to the technical field of microbial fermentation engineering and functional food application. The invention discloses a metagen product containing ergothioneine and achyranthes bidentata polysaccharide and capable of improving immunity. The metagen product is prepared from the following components in parts by mass: 80-100 parts of lactobacillus plantarum thallus powder containing ergothioneine, 10-16 parts of achyranthes bidentata polysaccharide, 0.5-1.5 parts of an immunoactivator, 20-40 parts of prebiotics, 3-5 parts of a sulfur element precursor and 1-10 parts of an emulsion stabilizer. And the specific immunoactivator and inactivated thalli form a dual anti-oxidation barrier, so that the oxidative degradation of ergothioneine is reduced, and the stability of the product is improved. The superfine ground thalli are matched with prebiotics and an emulsion stabilizer, so that release of active ingredients and absorption of intestinal tracts are promoted, and the bioavailability is improved. Multiple components are scientifically matched to achieve synergistic interaction, intestinal regulation and system immunity enhancement are both considered, and the efficacy is more comprehensive.
Owner:SHANGHAI ERGOTEIN BIOTECHNOLOGY GRP CO LTD