125 results about "Thallus" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to a postbiotic preparation for pets as well as a preparation method and application of the postbiotic preparation. The metabolite preparation provided by the invention comprises a lactobacillus plantarum Ps-8 inactivated thallus and a metabolite thereof, a lactobacillus casei C-9 inactivated thallus and a metabolite thereof, a bifidobacterium animalis subsp. Lactis BB-16 inactivated thallus and a metabolite thereof, and a lactobacillus acidophilus LA-14 inactivated thallus and a metabolite thereof. The pet food can be applied to various food types of different varieties of cat and dog pets, and has the effects of improving the intestinal barrier function of the pets, enhancing the immunity, reducing the incidence rate of diarrhea, reducing the food change stress, promoting the digestion of nutrient substances, optimizing the state of spirit and fur, reducing the odor of excrement, fading the odor of the oral cavity, improving the health level of the oral cavity and teeth and the like.

The utility model relates to the technical field of microbial fermentation control, and provides an automatic online detection and automatic feedback control system for a microbial fermentation process, which is characterized in that a ceramic membrane is arranged in a filter cavity, the filter cavity is divided into an inner cavity and an outer cavity by the ceramic membrane, and the inner cavity is connected with a fermentation tank through a material discharge pipeline and a thallus return pipeline respectively; the outer cavity is connected with the dilution mixing pool through the clear liquid discharging pipeline, the dilution water supply pipeline is connected with the dilution mixing pool, and the dilution mixing pool is connected with the sampling pool; the material supplementing pipeline is connected with the fermentation tank, and the control mainboard is configured to control the sampling assembly to act so as to suck feed liquid from the sampling pool, move the feed liquid to the detection assembly for detection and control the material supplementing pipeline to supplement materials to the fermentation tank according to a detection result of the detection assembly. Therefore, fermentation liquor in the fermentation tank cannot be polluted while sampling detection and material supplementation can be completed on line, the whole fermentation process is free of bacterial contamination risk, and the whole process is easy and convenient to operate and saves time and labor.

The invention discloses a compound bacterial fertilizer for reducing soil obstacles as well as a preparation method and application thereof, and relates to the technical field of biology. The compound bacterial fertilizer is prepared from a mortierella indurata bacterial agent, a trichoderma hedgehog bacterial agent, an osmanthus fragrans geotrichum bacterial agent and a bacillus cereus bacterial agent, the mortierella cinerea preparation is obtained by uniformly mixing a mortierella cinerea spore suspension and corn straw powder and then drying; the trichoderma hedgehog fungicide is obtained by uniformly mixing a trichoderma hedgehog spore suspension and corn straw powder and then drying; the osmanthus fragrans geotrichum fungicide is obtained by uniformly mixing osmanthus fragrans geotrichum spore suspension and corn straw powder and then drying, the bacillus cereus microbial inoculum is obtained by freeze-drying a bacillus cereus fermentation thallus. By applying the compound bacterial fertilizer to the surface layer of ginseng continuous cropping soil, the soil condition of the ginseng continuous cropping soil can be improved, ginseng growth is promoted, ginseng root rot is reduced, and the survival rate of ginseng plants is increased.

The invention relates to a recombinant thallus, a construction method of the recombinant thallus and application of the recombinant thallus in production of xanthoxylin WGX-50, and relates to the technical field of bioengineering. A recombinant plasmid containing a phenylalanine ammonialyase gene PAL with a nucleotide sequence as shown in SEQ ID NO.2 and a cinnamic acid coenzyme A ligase gene CNL with a nucleotide sequence as shown in SEQ ID NO.1 and a recombinant plasmid containing a tyramine N-hydroxycinnamoyl transferase gene THT with a nucleotide sequence as shown in SEQ ID NO.3 are introduced into the recombinant plasmid. A phenylalanine ammonialyase gene PAL, a cinnamic acid coenzyme A ligase gene CNL and a tyramine N-hydroxycinnamoyl transferase gene THT are overexpressed in escherichia coli, so that a biological synthesis path of xanthoxylin WGX-50 is formed by taking 3, 4-dioxomethyl phenylethylamine as a substrate for the first time, a foundation is laid for large-scale industrial production, and the method has a wide application prospect. The method has important economic value and social benefit.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of bacillus velezensis fermentation liquor in prevention and control of citrus red mites. A bacillus velezensis XZT106 strain is subjected to fermentation treatment to obtain fermentation liquor without thalli, the fermentation liquor contains effective components including epothilone A and silthiopham, experiments prove that the death rate of red spiders treated by a stock solution of the fermentation liquor is 100%, the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7%, and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 500 times and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7% respectively. In addition, it is further verified that the fermentation liquor of the bacillus velezensis XZT106 thallus does not generate the problems of drug resistance and environmental pollution in the process of preventing and treating the red spiders.

The invention provides a cold resistance comprehensive evaluation method for porphyra yezoensis, and belongs to the technical field of biological breeding. Four phenotypic indexes related to the cold resistance character are obtained through screening experiments, the four phenotypic indexes are the leaf area of the porphyra yezoensis thallus, the maximum light quantum efficiency Fv / Fm, the photosynthetic pigment chlorophyll a and allophycocyanin respectively, and the four phenotypic indexes can not only meet sustainable determination in a lossless state, but also can be used for detecting the cold resistance character of the porphyra yezoensis. And the cold resistance evaluation model constructed based on the few phenotypic indexes can achieve the purpose of accurately, efficiently and comprehensively evaluating the cold resistance of the porphyra yezoensis. Experimental results show that the method provided by the invention is relatively consistent with the cold resistance of an actual porphyra yezoensis variety or strain, the cold resistance grade of a to-be-detected sample can be accurately identified, the blank of cold resistance evaluation of the porphyra yezoensis is filled, and a technical basis is provided for subsequent screening, identification and breeding of a cold-resistant porphyra yezoensis culture variety.

The invention discloses a method for rapidly acquiring spore seedlings of meniscus violaceum, and relates to the field of asexual reproduction and breeding of laver. The method comprises the following steps: crushing laver to different degrees by using a wall breaking machine, crushing thalli into fragments with certain sizes, collecting the fragments, culturing the fragments for several days, and adding seedling ropes after the fragments develop into monosporangiums and start to diffuse monospores. The seedling ropes and the fragments are mixed and subjected to aerobic culture, and a large number of monospore seedlings can be obtained. According to the mechanical crushing method, a manual chopping crushing method can be replaced, the crushing efficiency of the laver is greatly improved, and large-scale induction and diffusion of the purple menu spores can be realized. Meanwhile, by conducting joint aerobic culture on the fragments and the seedling ropes, the step of collecting the monospore seedlings can be simplified, a large number of monospores are effectively attached to the seedling ropes and rapidly develop into the monospore seedlings, and large-scale monospore seedling culture becomes possible.

The invention relates to the technical field of genetic engineering, in particular to application of a knockout Fum gene in increasing the yield of erythritol of a strain. The invention provides application of knockout of a Fum gene in a strain in increasing the erythritol yield of the strain, the amino acid sequence of protein coded by the Fum gene is shown as SEQ ID NO: 1, and the strain comprises yarrowia lipolytica. A Fum gene for coding fumarase in a yarrowia lipolytica genome is knocked out by utilizing a CRISPR (clustered regularly interspaced short palindromic repeats) technology, a recombinant yarrowia lipolytica CICC1457-delta Fum with obviously improved erythritol yield is obtained, the erythritol yield is improved by 75.7% and reaches 4.71 g / L compared with that of the original yarrowia lipolytica CICC1457, and in the fermentation culture process, the yield is increased by 75.7% and reaches 4.71 g / L; the thallus growth speed and thallus biomass are not obviously different from those of an original strain.

The invention belongs to a microbial technology, and particularly relates to bifidobacterium longum ZFML006 and an anti-aging function and application of inactivated thalli of the bifidobacterium longum ZFML006. The invention discloses Bifidobacterium longum ZFML006, and the preservation number of the Bifidobacterium longum ZFML006 is CCTCC (China Center for Type Culture Collection) NO: M 2025331. The invention also provides an inactivated thallus prepared from the bifidobacterium longum ZFML006, and the inactivated thallus can be used for preparing anti-aging drugs and also has an anti-oxidation effect.

The invention belongs to the field of microbial culture, and particularly relates to an enrichment medium for reducing expression of surface polysaccharide of vibrio parahaemolyticus. According to the formula of the polysaccharide low-expression enrichment culture medium, nutritional ingredients in a specific proportion and specific polysaccharide expression inhibition conditions are selected, so that the expression quantity of surface lipopolysaccharide and capsular polysaccharide after enrichment of vibrio parahaemolyticus is lower than the expression quantity of polysaccharide after enrichment of normally cultured vibrio parahaemolyticus; expression of lipopolysaccharide and capsular polysaccharide on the surface layer can be inhibited to a certain extent, so that outer membrane protein can be better exposed on the surface of a thallus, and immunodetection of vibrio parahaemolyticus is facilitated. The invention provides a technical basis for developing a complete vibrio parahaemolyticus immunodetection method.

The invention belongs to the technical field of biomedical treatment and biological fermentation, discloses a metagen composition for effectively inhibiting oral dental caries and application thereof, and aims to solve the problems of great side effect, weak effect and unknown mechanism of the existing dental caries prevention and treatment means. The composition is prepared by mixing a metagen 1 which is prepared by fermenting lactobacillus salivarius Postbio-YY with a metagen 2 which is prepared by fermenting lactococcus lactis Postbio-F3 and lactobacillus paracasei Postbio-P6 step by step. In the preparation process, the active ingredients are reserved by accurately controlling the fermentation pH, time and pasteurization parameters. The composition can be prepared into multiple dosage forms such as toothpaste and the like, can remarkably inhibit the growth of streptococcus mutans and destroy the thallus structure, inhibits biofilm and exopolysaccharide synthesis by down-regulating fimI, wcaJ and wcae genes, is safe and free of side effects, and provides an efficient scheme for decayed tooth prevention and treatment.

The invention belongs to the technical field of artificial cultivation of cordyceps sinensis, and particularly relates to a method for rapidly propagating and culturing cordyceps sinensis based on cordyceps sinensis infection bodies, which comprises the following steps: (1) inoculating cordyceps sinensis into a cordyceps sinensis culture solution for culturing to obtain a cordyceps sinensis infection body culture solution; the cordyceps sinensis is a cordyceps sinensis thallus separated from infected hepialus armoricanus larva or a cordyceps sinensis solid culture which has produced conidia; and (2) mixing the winter worm summer fungus infection body culture solution with the hepialus armoricanus larva feed, feeding the hepialus armoricanus larvae, and carrying out grass emergence culture after the hepialus armoricanus larvae are fed to be rigid. According to the method, the rapid propagation culture infection body is high in concentration (can reach 3.3 * 10 < 7 > / mL), short in period (about 40 days) and high in infection rate (not lower than 80%), a large number of cordyceps sinensis infection bodies can be obtained in a short time, the cordyceps sinensis infection bodies are used for infecting cordyceps sinensis hosts to obtain cordyceps sinensis, and the problems that the source of artificially matched and planted cordyceps sinensis strains is unstable, and strain preparations are difficult to store for a long time can be solved.

The invention relates to a method for inhibiting rumen gas production by using a genetically modified laver filament as a ruminant feed additive and a method for inhibiting methane production by using the genetically modified laver filament as the ruminant feed additive. A porphyra yezoensis bromine peroxidase gene is cloned, an overexpression vector of the gene is constructed, porphyra yezoensis thallus is used as a receptor for genetic transformation, and a porphyra algal strain of which the key enzyme expression quantity is remarkably improved is obtained through screening. The method comprises the following steps: inducing thalli to generate protonema, crushing the protonema by using a crusher, carrying out ventilated large-scale culture in a seawater culture medium added with PES by using a column bioreactor to obtain a large number of protonema materials, collecting the protonema materials, crushing the protonema materials by using the crusher, and drying at low temperature. The obtained laver protonema powder is put into a vacuum bag for low-temperature preservation in dark light, and can be applied to preparation of ruminant feed as a dairy cow feed additive. The filamentous can accumulate halogenated compounds such as bromidogen and the like, can inhibit the activity of a key enzyme generated by methane in rumen, and further inhibits the generation of methane.

The invention relates to the technical field of agricultural product management and control, in particular to a photovoltaic-edible mushroom collaborative intelligent agricultural industry fusion management and control method and system, and provides the following scheme: generating an input data set for energy-production collaborative analysis by obtaining multi-source monitoring data of a photovoltaic power generation and edible mushroom production unit; the physiological critical period of the edible mushrooms is recognized based on manifold embedding and topological matching algorithms, an anti-fact manifold matching model under energy constraint is constructed, and verifiable judgment of physiological state changes is achieved; dynamically evaluating the stability and energy consumption efficiency of the thalli in combination with a stage perception and energy generation mapping empirical model; and a hierarchical multi-objective optimization algorithm is adopted to generate a regulation and control scheme considering the energy utilization efficiency and the thallus stability, and the regulation and control scheme is output to an energy management end. According to the method, the collaborative optimization of the thallus growth stability and the system energy efficiency can be kept in the fluctuating energy environment, and the method is suitable for photovoltaic agriculture, intelligent edible mushroom factories and new energy-biological coupling type agriculture scenes.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation method for increasing the content of pyocyanin in pseudomonas aeruginosa fermentation liquor. The invention provides a novel pseudomonas aeruginosa fermentation method. The multiplication capacity and metabolic activity of thalli are regulated and enhanced through a microgravity environment. According to the method, single colonies of pseudomonas aeruginosa are cultured through streak isolation, activated and then inoculated to a fermentation medium, fermentation is performed under the microgravity condition of 0.001 g, the microgravity environment fermentation remarkably optimizes thallus growth, the pyocyanin yield is increased, and the biofilm forming ability is enhanced.

The utility model discloses an edible fungus incubator networking alarm device, which relates to the technical field of edible fungus cultivation, comprises a box body, and is characterized in that a box door is arranged on one side of the box body, a sealing gasket is arranged on one side, close to the box body, of the box door, and three mounting blocks are arranged on each of two sides of the inner wall of the box body. And draining plates are arranged among the three groups of mounting blocks. The sealing effect between the box body and the box door can be improved through the arranged sealing gasket, external dust and impurities are prevented from entering the box body to influence thallus embryos, when the box body needs to be atomized and humidified, some small water drops in the culture groove are gathered together, water can fall into the draining groove through the draining holes, and therefore the thallus embryos are prevented from being damaged. Therefore, some water can be prevented from being deposited in the culture tank for a long time to influence thalli in the culture tank, the quality of produced edible mushrooms is ensured, the culture plate can be taken out by pulling the handle, the water collected in the draining tank can be conveniently recycled, and waste of water resources is avoided.

The invention discloses a cordyceps sinensis culture control method and system, and core culture targets are set in generations by identifying the subculture generations G1, G2 and G3 of cordyceps sinensis strains: the G1 generation activates a cordycepin and adenosine synthesis pathway, the G2 generation maximizes the total nucleoside content and antioxidant activity, and the G3 generation improves the biomass and delays degradation. The system monitors environmental parameters such as temperature and humidity, illumination, gas concentration and the like, and thallus physiological indexes such as biomass, metabolism precursor concentration, active substance content and the like in real time, and adopts algorithms such as fuzzy PID, NSGA-II multi-objective optimization, model prediction control and the like to dynamically adjust culture conditions such as nutrition supply, dissolved oxygen, illumination period and the like. The method provided by the invention solves the problem that traditional static regulation neglects intergenerational difference of strains, realizes subgeneration refined targeted regulation, remarkably improves the yield and quality of target products, prolongs the service cycle of the strains, and is suitable for industrial bionic cultivation of cordyceps sinensis.

The invention discloses an extraction method of high-purity glutamic acid, and belongs to the field of monosodium glutamate production industry. The method comprises the following steps: introducing glutamic acid fermentation liquor into an ultrafiltration membrane to obtain clear liquor and thallus concentrated liquor A; the thallus concentrated solution A enters a dialysis membrane to obtain dialysate and a thallus concentrated solution B; carrying out filter pressing on the thallus concentrated solution B to obtain fungi residues and filtrate; merging the filtrate and the thallus concentrated solution A; the dialysate enters a concentration membrane, a concentrated solution and membrane produced water are obtained, and the membrane produced water is dialysis water; the concentrated solution and the clear solution enter a continuous isoelectric crystallizer to be crystallized, and glutamic acid crystal slurry is discharged at the same time for crystallization; performing primary separation to obtain glutamic acid and isoelectric mother liquor A; the isoelectric mother liquor A is subjected to secondary separation, fine glutamic acid crystals and isoelectric mother liquor B are obtained, and the isoelectric mother liquor B enters a sewage treatment procedure; and fine glutamic acid crystals return to the continuous isoelectric crystallizer. The glutamic acid obtained by the method has the advantages of high yield, high product purity, high light transmittance and good color and luster, and is superior to the commonly used'concentration continuous isoelectric crystallization + crystal transformation 'process.

The invention provides a thallus water content detection method and grading method, which comprises the following steps: irradiating a light source comprising a plurality of spectral components with different wavelengths to a unit area region of thallus, so that the thallus transmits reflected light back to a probe, and the probe transmits the reflected light to a spectrograph to generate a reflected light spectrum; on the basis of a spectrum-water content mapping model, the water content of the thallus is determined according to the intensity characteristics of the sensitive wave band in the reflected light spectrum, and in the spectrum-water content mapping model, when the water content of the thallus is different, the intensity of single-wavelength reflected light in the sensitive wave band of the spectrum is different; when the water content of the thalli is certain, the intensities of reflected light with different wavelengths in the sensitive wave band of the spectrum are different, and the intensity difference value of the reflected light with different wavelengths is related to the water content of the thalli and the wavelength difference value of the reflected light. The water content of the thalli can be accurately and rapidly measured, and the classification accuracy of the thalli can be improved.

The invention relates to the technical field of biological product processing equipment, and discloses equipment and a method for freeze thawing of thalli, the equipment comprises a cavity, a refrigeration system, a temperature probe, a slide rail, a loading trolley and a control system, the cavity is provided with double doors and an air return wall; the refrigerating system comprises compression condensing units symmetrically arranged outside the cavity, circulating fans and evaporators symmetrically arranged on the two sides of the top of the cavity, automatic telescopic curtains are arranged below the circulating fans and stretch across the cavity, temperature probes are arranged on the inner wall of the air return wall, sliding rails penetrate through the bottom of the cavity, and rolling wheels are arranged at the bottom of the loading trolley and matched with the sliding rails; and the control system receives the temperature information to control the refrigerating system. The invention further provides a thallus freezing and thawing method, uniform control over the temperature field in the cavity and zero interference in the defrosting process are achieved through cooperative work and alternate defrosting of the double refrigeration units, and the thallus freezing and thawing method has the advantages of being easy and convenient to operate, automatic in the whole process, efficient in operation, safe in redundancy and the like.

The invention belongs to the technical field of microorganisms, and particularly relates to a bacteriophage for splitting bovine-derived salmonella and application of the bacteriophage. The technical problem to be solved by the invention is to provide a new choice for treating infection of bovine-derived salmonella. According to the technical scheme, the invention relates to a bacteriophage Seoulvirus GX2401 for splitting bovine-derived salmonella, wherein the preservation number of the bacteriophage Seoulvirus GX2401 is CCTCC (China Center for Type Culture Collection) NO: M20241156. The bacteriophage is a wide host spectrum thallus, can split six different salmonella, has a wide antibacterial spectrum, and can be applied to preparation of drugs for preventing and treating bovine salmonella infection diseases.

The invention discloses a transcription factor HbNAC47 related to saline-alkaline tolerance, and a coding gene and application thereof. The amino acid sequence of the transcription factor HbNAC47 related to the saline-alkaline tolerance character is as shown in SEQ ID No. 1. The nucleotide sequence of the coding gene of the transcription factor HbNAC47 related to the saline-alkaline tolerance character is as shown in SEQ ID No. 2. The transcription factor HbNAC47 or the coding gene thereof is applied to cultivation of saline-alkaline tolerant transgenic plants. The invention further provides a recombinant expression vector, an expression cassette or a recombinant thallus of the coding gene of the transcription factor HbNAC47 related to the saline-alkaline tolerance character and application of the recombinant expression vector, the expression cassette or the recombinant thallus in cultivation of saline-alkaline tolerant transgenic plants. By overexpressing the barley grass HbNAC47 gene, the expression of salt-alkali tolerant related genes is regulated, so that a salt-alkali tolerant plant is obtained, and the barley grass HbNAC47 gene has great production and application values.

The invention discloses a non-sterilization type mildew-proof and antibacterial material capable of dynamically responding to thallus proliferation as well as a preparation method and application of the non-sterilization type mildew-proof and antibacterial material. The material comprises polyvinyl alcohol, carboxymethyl cellulose or a salt thereof and metal ions, and the polyvinyl alcohol, the carboxymethyl cellulose or the salt thereof are bridged through the metal ions; the metal ions comprise Li < + >. The material disclosed by the invention takes Na < + > released by thallus proliferation as a signal medium, releases Li < + > as required through a coordination-dissociation process, and further induces thallus cells to actively ingest Li < + > through metabolic deception of occupying a potassium ion channel by utilizing a hydration radius of Li < + > similar to K < + > according to the metabolic demand of sodium excretion and potassium ingestion in the thallus proliferation process; on one hand, bacterial cell replication lacks essential nutrient substance K < + >, and finally gradually declines; on the other hand, protein space conformation is changed and inactivated by capturing water molecules of intracellular protein, and the two aspects have a synergistic effect, so that the efficient mildew-proof and antibacterial effects of Li < + > at low concentration can be realized, and the effect is lasting.

To provide a technique for suppressing maturation of seaweed.SOLUTION: The foregoing problem is solved by a seaweed culturing method comprising a step of culturing seaweed under supply of microbubbles and a step of collecting the seaweed.SELECTED DRAWING: None

The invention discloses a screening method and application of a floating moss endogenous constitutive promoter, and belongs to the technical field of gene engineering. A floating moss endogenous constitutive promoter is screened out through experiments such as screening verification of candidate constitutive promoters, a plant expression vector of which a reporter gene started by the promoter is positioned in a cell nucleus is constructed, and floating moss is transformed to obtain a transgenic plant. The fluorescence intensity of the reporter gene driven by the promoter is higher than that of other common constitutive promoters, such as CaMV35S and the like, and meanwhile, when fluorescence expression of transgenic plants is observed, it is found that the reporter gene can be stably expressed in thalli, scales, various different types of cells of the thalli and the scales and the like. The invention provides an efficient and universal core tool for floating moss gene function research, genetic manipulation, biotechnology application and the like, and has a certain application prospect.

The invention relates to a method for preparing pentamethylene diamine through continuous conversion and a production strain thereof. The invention belongs to the technical field of microorganisms, and particularly relates to a genetically engineered bacterium capable of continuously producing pentamethylene diamine and application of the genetically engineered bacterium. According to the pentamethylene diamine biotransformation genetically engineered bacterium disclosed by the invention, kdsD, lpxL, pagP, lpxP, eptA, lpxM, OmpF and OmpC are deleted, so that the thallus can be self-settled after the transformation of the strain is completed, convenience is provided for subsequent continuous transformation and extraction and purification of pentamethylene diamine, the accumulation of pentamethylene diamine reaches a relatively high level after multiple continuous transformation processes, and the cost is saved. The invention also provides a thallus sedimentation mode, which is characterized in that 10-20 mM of Ca < 2 + >, Mg < 2 + > and 5-40 g / L of LMat01 are added on the basis of the genetically engineered bacterium disclosed by the invention, so that thalli can be completely settled within 15 minutes, and can still be completely settled within 15 minutes after multiple times of transformation. According to the invention, by establishing a thallus sedimentation process, a strain required by synthesis of pentamethylene diamine and an efficient preparation method thereof are constructed, so that a continuous preparation process of pentamethylene diamine is realized.

The utility model relates to the technical field of filter materials, and discloses a filter material easy for thallus growth, which comprises an outer cylinder and a filter material cylinder, the filter material cylinder is arranged in the outer cylinder, a large opening is arranged on the end face of the outer cylinder, a small opening is arranged on the end face of the filter material cylinder, a vertical frame is arranged on the outer ring of the filter material cylinder, and an annular frame is arranged on the vertical frame. The filter material barrel is arranged on the outer barrel, the filter material barrel can be installed on the outer barrel through a large screw head, the extension seat is arranged on the filter material barrel, the vertical frame is arranged on the extension seat, the convex seat is arranged on the annular frame corresponding to the vertical frame, the guide opening is formed in the convex seat, and the annular frame can slide on the vertical frame through the convex seat. The annular frame and the vertical frame can be combined into a frame by arranging the annular frame and the vertical frame, so that a position where thalli can be attached is provided, and growth of the thalli can be facilitated by arranging the annular frame and the vertical frame.

The invention discloses a hydrogel and probiotic compounded microbial inoculum composition and a vegetable planting matrix, a three-dimensional gel carrier is used for embedding a microbial inoculum, the carrier integrates mechanical properties and thallus protection and can maintain flora activity for a long time, bacillus thuringiensis has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression, and the microbial inoculum composition has the advantages that the microbial inoculum composition has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression; the microbial agent composition can survive for a long time in a low-oxygen environment of a carrier embedding environment, and is cooperated with bacillus cereus, azotobacter chroococcum and bacillus megatherium to promote the growth of plants such as vegetables and the like and directionally kill pathogenic microorganisms such as root-knot nematode. Corresponding pests can be killed in a green and targeted manner, the growth of plants is promoted, and the yield is increased.

The invention discloses a stable genetic transformation method of floating moss. The genetic transformation method comprises the following steps: (1) crushing thallus tissues of the floating moss, (2) pre-culturing explants, (3) infecting and co-culturing, (4) screening and culturing for the first time, (5) screening and culturing for the second time, (6) enlarging and culturing, and (7) verifying transformation. According to the genetic transformation method, a thallus of floating moss is taken as a starting explant, an agrobacterium-mediated hygromycin and kanamycin screening transformation system of the floating moss is established for the first time, the blank of a floating moss genetic transformation system is filled, and experimental results show that after multi-generation culture, a transformed plant can still stably express a target gene. The method has the advantages of high transformation efficiency and simplicity in operation, and can be used for subsequent genetic transformation research of unknown floating moss genes. The method has a wide application prospect.

The invention discloses a method for improving the high-temperature survival rate of bacillus cereus. The method comprises the following steps: adding a stabilizer into a bacterial liquid obtained by fermenting the bacillus cereus; the stabilizer is a sterile aqueous solution prepared from one or more of trehalose, 2-nitrogen hexanone, gallic acid, glycerol, 3-phenylpropionic acid, cane sugar and L-alanine. By adding the stabilizer, the high-temperature thallus survival rate of the bacillus cereus is improved, the application field of the bacillus cereus is widened, and the limitation of temperature on the application of the bacillus cereus is effectively solved.