612 results about "Microfiltration" patented technology

The invention discloses a purification device for aquaculture wastewater, and relates to the technical field of wastewater purification. Comprising a box body as well as a physical filter tank and a biochemical bacterium culture tank which are arranged in the box body, and further comprises a microfiltration assembly arranged in the physical filter tank, the microfiltration assembly comprises a filter cartridge rotationally connected to the interior of the physical filter tank, a backwashing pipe is fixedly connected to the interior of the physical filter tank, and a sewage collecting tank is arranged in the filter cartridge; a cleaning assembly matched with backwashing to improve the cleaning effect of the filter cartridge is arranged in the filter cartridge, the cleaning assembly comprises a floating plate arranged in the filter cartridge, a connecting rod slidably penetrates through the floating plate, and the upper end of the connecting rod penetrates through the dirt collecting tank and is fixedly connected with a first cleaning brush; stubborn attachments such as mycoderm and caustic soda attached to the inner wall of the filter cartridge can be brushed off by utilizing the first cleaning brush, and the cleaning effect on the filter cartridge can be improved by matching with backwashing, so that the attachments on the inner wall of the filter cartridge can be massively washed off, the backwashing period is shortened, and the blockage probability of the filter cartridge is reduced.

The invention belongs to the technical field of protein purification processes, and particularly relates to a method for separating and purifying alpha-lactalbumin in whey. Comprising the following steps: S1, obtaining skimmed milk; s2, performing microfiltration separation on the skimmed milk obtained in the step S1 to obtain whey protein liquid containing alpha-lactalbumin; s3, loading the whey protein liquid containing alpha-lactalbumin obtained in S2 into a balanced strong cation chromatographic column, and collecting a flow-through liquid to obtain separated and purified alpha-lactalbumin; according to the method, the SP Purose 6XL filler is combined with the specific 10-60mM acetic acid buffer solution with the pH value of 4-6, so that the method is particularly suitable for separating the alpha-lactalbumin from other proteins in the whey, and the alpha-lactalbumin is collected in a flow-through manner, so that large-volume sample loading can be realized, and more importantly, the obtained alpha-lactalbumin is high in yield and purity; in addition, the process is simple in extraction step and free of a tedious elution process, a large amount of time can be saved, the production cost is reduced, and industrial production is facilitated.

The utility model discloses a crystal film fluidized bed treatment system aiming at high-hardness water treatment, the system mainly comprises a crystallization fluidized bed and a multi-layer flat film microfiltration device, and the crystallization fluidized bed device is of an inner-layer and outer-layer cylinder structure and is provided with a slag discharge pipe, a water inlet pipe, an annular dosing pipe, an inclined pipe enhanced separation device and a water outlet pipe; the multi-layer flat membrane microfiltration equipment is provided with a water inlet pipe, a water outlet pipe, a buffer water tank, a cleaning mechanism, a metal membrane, a sludge tank and the like; when the system runs, high-hardness raw water firstly enters the crystallization fluidized bed for softening treatment, and then enters the multi-layer flat membrane microfiltration equipment for further removing hardness and turbidity after treatment; the adopted system combining the crystallization fluidized bed and the flat membrane microfiltration equipment can effectively remove the hardness and turbidity of water, flocculation and coagulation agents do not need to be added, the adverse effect of the coagulation agents on a subsequent membrane system is effectively avoided while the operation cost of the system is reduced, the hardness and turbidity of the water can be effectively removed, and the service life of the system is prolonged. Various ions in the water are synchronously removed.

The invention discloses a protein separation method and related application thereof, and relates to the technical field of dairy product processing, the method comprises the following steps: heating a whey solution of which the pH value is adjusted to 3.5-4.2 by a citric acid solution, carrying out microfiltration on the heated product, carrying out beta-lactoglobulin and / or alpha-lactalbumin separation on permeate and trapped fluid obtained by microfiltration, the method is based on the membrane filtration technology, high-purity separation of beta-lactoglobulin and alpha-lactalbumin is achieved, the purity can reach 70%-90% or above, the method is compatible with whey from multiple sources, equipment is simple, the method has the advantages of being low in separation cost, high in efficiency, easy to implement, large in flux and the like, and a way is provided for large-scale industrial production of beta-lactoglobulin and alpha-lactalbumin.

The invention provides an extraction method and application of a sugar-controlling component in mulberry leaves, and the extraction method comprises the following steps: mixing mulberry leaf powder and an acidic ethanol solution, and then carrying out ultrasonic extraction to prepare a first extracting solution and a first extracting residue; carrying out enzymolysis treatment on the first extraction residue, and collecting a second extraction solution; combining the first extracting solution and the second extracting solution, concentrating and decoloring to obtain a decolored solution; carrying out microfiltration, nanofiltration and membrane concentration on the decolored solution, and then drying; the enzymolysis treatment comprises primary enzymolysis treatment and secondary enzymolysis treatment; a composite decolorizing agent is added in the decolorizing process; the preparation method of the composite decolorizing agent comprises the following steps: mixing the activated carbon and the attapulgite, pickling, and calcining to obtain a first mixture; mixing and reacting the first mixture, chitosan and acetic acid. According to the method, the extraction process of the mulberry leaf powder is controlled, so that the sugar-controlled component in the mulberry leaves is efficiently extracted.

The invention relates to the technical field of environmental engineering, in particular to an aerobic granular sludge sewage treatment process which comprises the following steps: S1, performing ultrasonic treatment on raw sewage; s2, preliminarily screening the sewage subjected to ultrasonic treatment; s3, adjusting the pH value of the screened sewage, and adding nutrient substances; s4, injecting the adjusted sewage into a biological reaction zone containing pre-cultured aerobic granular sludge, and carrying out intermittent aeration operation; s5, regularly performing microwave treatment in the biological reaction process; s6, carrying out chemical precipitation on the sewage subjected to biological treatment; s7, performing microfiltration on the sewage subjected to biological treatment and chemical treatment; s8, performing ozone and ultraviolet light treatment on the water subjected to microfiltration; s9, carrying out tail end disinfection on the treated water; according to the invention, the formation and stabilization of aerobic granular sludge are effectively promoted, the removal efficiency of pollutants such as organic matters, nitrogen and phosphorus in sewage is improved, and the effluent quality is ensured to reach the standard.

The invention belongs to the technical field of aquaculture equipment, and particularly relates to circulating water microfiltration equipment and a cleaning method thereof. Circulating water microfiltration equipment comprises a first shell, a filtering assembly, a cleaning assembly and a dirt collecting assembly. The filtering assembly is used for filtering tail water. The cleaning assembly comprises a water inlet pipe and a cleaning pipeline, the cleaning pipeline is provided with a plurality of cleaning nozzles, and the plurality of cleaning nozzles are all aligned with the filter vat and can perform back-flushing operation on the filter vat. The dirt collecting assembly comprises a dirt collecting frame, a scraping plate and a dirt collecting box. The dirt collecting frame is used for collecting impurities on the filtering barrel flushed by the cleaning assembly, and the dirt collecting box is located below the dirt collecting frame and used for collecting the impurities scraped by the scraping plate. Therefore, the filter barrel can be quickly and effectively cleaned, the filter barrel cannot be blocked, and the technical problems that in the prior art, a filter screen is blocked due to long-term operation of a microfilter, and the filter screen is not easy to clean in the prior art are effectively solved.

The invention discloses a siraitia grosvenorii low-temperature continuous countercurrent extraction process and a siraitia grosvenorii extract, and belongs to the technical field of siraitia grosvenorii extraction.The siraitia grosvenorii low-temperature continuous countercurrent extraction process comprises the following steps that S1, fresh siraitia grosvenorii fruits are extracted through a four-stage squeezing and continuous countercurrent extraction combined method, s2, carrying out solid-liquid separation on the first liquid phase through ceramic membrane microfiltration, and collecting a microfiltrate; and S3, carrying out post-treatment on the micro-filtrate to obtain the extract. The siraitia grosvenorii low-temperature continuous countercurrent extraction process is high in extraction efficiency and low in solvent consumption, and the prepared extract has both high sweetness and oxidation resistance.

The invention discloses a preparation method of a raspberry-derived exosome loaded with neroli essential oil. The preparation method comprises the following steps: preparing raspberry fruits into homogenate; centrifuging, collecting supernate, adjusting the pH value to 4.07.5, standing, and centrifuging again; taking supernate, adjusting the pH value to 7.2-7.4, adding polyethylene glycol, standing, centrifuging, collecting precipitate, and carrying out resuspension dispersion; performing microfiltration to obtain supernate, and combining electrophoresis dialysis and ultrafiltration concentration to obtain the high-purity raspberry exosome. And diluting the exosome, adding the diluted exosome into a dimethyl sulfoxide solution containing neroli essential oil, carrying out water bath stirring, carrying out ultrafiltration centrifugation, and taking a clear liquid to obtain the exosome loaded with the neroli essential oil. Efficient separation and purification of the raspberry exosome are achieved by applying an isoelectric precipitation technology, then the neroli essential oil is wrapped in the exosome, the stability of the essential oil is improved, the action time is prolonged, meanwhile, the neroli essential oil and active ingredients of the exosome generate a synergistic effect, and the antioxidant activity is jointly enhanced.

The invention relates to the technical field of biological medicine, in particular to a lactobacillus-sourced extracellular vesicle and application of the lactobacillus-sourced extracellular vesicle in prevention and treatment of necrotizing enterocolitis, the extracellular vesicle is prepared from lactobacillus, the lactobacillus is Lactobacillus rhamnosus GG, the strain is purchased in the American Center for Type Culture Collection, and the preservation number is ATCC 53103. The preparation method of the extracellular vesicles comprises the following steps: carrying out ultracentrifugation on a lactobacillus culture solution, removing thalli, then collecting supernate, carrying out primary microfiltration, taking micro-filtrate, carrying out differential centrifugation, resuspending precipitates by using PBS, then carrying out secondary microfiltration, collecting the micro-filtrate, carrying out density gradient centrifugation, and collecting a 30-45% interface vesicle layer. The invention discloses a synergistic effect mode between intestinal symbiotic flora and intestinal epithelial cell metabolic molecules for the first time, and the discovery is expected to construct a brand-new mechanism normal form for regulating the host intestinal barrier function by flora.

The invention relates to a skin-cleaning composition and application thereof in cleaning cream, and belongs to the technical field of daily chemicals, the skin-cleaning composition comprises the following raw materials: plant fermentation liquor, a chrysanthemum artemisia extract, silk amino acid, cetyl alcohol ethyl hexanoate, PEG-20 glyceryl triisostearate and soapberry seed oil; the preparation method of the plant fermentation liquid comprises the following steps: S1, pretreating part of the raw materials; s2, activating and culturing the strain; and S3, adjusting the pH value of the mixed solution A, performing temperature-controlled sterilization, cooling, adding mannase, performing temperature-controlled enzymolysis, performing enzyme deactivation after enzymolysis is completed, so as to obtain a mixed solution B, cooling, adding a lactobacillus acidophilus fermentation seed solution and a streptococcus thermophilus fermentation seed solution, performing temperature-controlled standing fermentation, performing temperature-controlled sterilization and speed-controlled centrifugation after fermentation is completed, taking supernate, and performing 0.22 mu m membrane microfiltration, so as to obtain the plant fermentation liquor. The raw materials of the skin-cleaning composition provided by the invention have a synergistic effect, so that the skin-cleaning composition has the effects of moisturizing and whitening while effectively cleaning the skin.

A method for producing a fluoropolymer aqueous dispersion, including a step A of subjecting a pre-treatment aqueous dispersion containing a fluoropolymer obtained by polymerization in the presence of a hydrocarbon surfactant to ultrafiltration, microfiltration, or dialysis membrane treatment, or a combination thereof. The ultrafiltration is performed using an ultrafiltration membrane having a molecular weight cutoff of 0.3×104 Da or more.

The invention provides a mulberry leaf extract rich in sugar inhibition components and a preparation method and application thereof, and the method comprises the following steps: S1, after mulberry leaves are subjected to water extraction, collecting a first water extract and water extraction residues; s2, fermenting the water extraction residues, performing water extraction, and collecting a second water extraction solution; s3, mixing the first water extract and the second water extract, and then performing flocculation, microfiltration, ultrafiltration, membrane concentration and drying; a flocculating agent in the flocculation process is modified chitosan; the modified chitosan is prepared from the following raw materials in parts by weight: 10 parts of chitosan, 1-2 parts of aldehyde sucrose and 3-4 parts of amino polyethylene glycol. According to the preparation method, the steps of the preparation process are controlled, so that the mulberry leaf extract with high sugar inhibition component content is prepared.

The invention relates to the field of fish and vegetable comprehensive cultivation, in particular to a photovoltaic fish and vegetable symbiotic system which comprises a photovoltaic system, a fishery breeding system and a circulating purification system, a fishpond is arranged below a photovoltaic panel, net cages are arranged in the fishpond for fish breeding, the circulating purification system is arranged around the fishpond, and water is pumped in the fishpond through a pump station; water quality purification and water quality maintenance are carried out on the pumped water through a precipitation filtration system, a microfilter, a hydroponic vegetable system and an ultraviolet disinfection system in sequence, so that the water body in the pond is ensured to fully and circularly flow, and the concentration of chemical substances such as ammonia nitrogen, nitrite nitrogen and the like which are harmful to fishes is reduced. Through the synergistic effect of all parts of the photovoltaic fish and vegetable symbiotic system, the problem that the water quality in the aquaculture environment under the photovoltaic panel is prone to deterioration is effectively solved, and the aquaculture benefits are improved.

The invention provides a method for separating beta-casein from a dairy product, a method for separating kappa-casein and a casein mixture. The method for separating the beta-casein from the dairy product comprises the following steps: separating whey and casein in skimmed milk to obtain a casein concentrated solution; carrying out low-temperature microfiltration-percolation treatment on the casein concentrated solution at the temperature of 10 DEG C or below to obtain beta-casein permeate and a by-product of which at least part of beta-casein is removed; and heating the beta-casein permeate to realize self-association of the beta-casein permeate, and then carrying out ultrafiltration-percolation to obtain the beta-casein. The influence on the structure and performance of the beta-casein in the separation process is reduced, and the purity of the separated beta-casein product is improved.

The utility model discloses a cell filtering and collecting bag. A cell filtering and collecting bag comprises a collecting bag body, a feeding pipe is installed at the top of the collecting bag body, a discharging pipe is installed at the bottom end of the collecting bag body, a filtering net is installed in the collecting bag body, an air pipe is installed on the collecting bag body, a microfiltration filter element is installed on the air pipe, and a microfiltration filter element is installed on the microfiltration filter element. A first control valve and a second control valve are respectively mounted on the feeding pipe and the discharging pipe. According to the cell filtering and collecting bag provided by the utility model, the collecting bag body, the feed pipe, the filter screen, the discharge pipe, the first control valve, the second control valve, the air pipe and the microfiltration filter element are arranged, so that continuous and closed operation of a cell suspension can be realized, and the microbial pollution risk of the cell suspension is reduced.

The invention discloses a method for preparing low-bitterness micromolecular bovine colostrum protein peptide powder by adopting a multi-stage membrane separation and enzyme membrane coupling combined technology, and belongs to the technical field of food processing. The preparation method comprises the following steps: firstly, dividing a colostrum component into components to be hydrolyzed by adopting a multi-stage membrane separation technology, namely casein intercepted by ceramic membrane microfiltration and lactoglobulin and lactalbumin filtered by an ultrafiltration membrane, and non-hydrolytic components, namely immune globulin and lactoferrin; the preparation method comprises the following steps: firstly, separating bovine coloctrum, then deeply hydrolyzing the separated lactoglobulin, milky white and casein flocculate with the molecular weight of less than 50kDa by adopting an enzyme membrane coupling technology, mixing with unhydrolyzed immunoglobulin and lactoferrin, and freeze-drying, so that the peptide content in the treated bovine coloctrum is more than 40%, the peptide content of 200-1000Da is more than 30%, the peptide content of 200-1000Da is more than 30%, and the peptide content of 200-1000Da is more than 30%. The preparation method is simple, the preparation dispersion time is within 10 s, no obvious bitter taste exists, the retention rate of immune globulin in bovine coloctrum reaches 90% or above, and the preparation method is suitable for large-scale industrial popularization.

The technical scheme of the utility model discloses a land-based canvas pool litopenaeus vannamei recirculating aquaculture system which comprises an aquaculture pool, and the edge of the bottom of the aquaculture pool inclines towards the center so as to enable aquaculture water and impurities to converge towards the center to a fish closestool. The settling pond is connected with an outlet of the fish closestool through a drainage pipe, and the fluidized bed is connected with the settling pond through a water guide pipe arranged at the water outlet channel; and the microfiltration part is connected with the fluidized bed and the culture pond through a water conduit and a water return pipe respectively. The bottom of the culture pond is inclined, water is drained from the center of the culture pond and enters the culture pond tangentially, so that vortexes are formed in the culture pond, and residual feed, excrement and the like are quickly concentrated towards the water outlet in the center of the bottom of the culture pond under the action of tangential force of water flow. Through a plurality of water inlet pipes and water outlet pipes, the effect of water quality circulation of the culture pond, the sedimentation pond, the fluidized bed biological filter and the rotary drum type micro-filter is achieved, and the problems that suspended solids and ammonia nitrogen are prone to being generated in a traditional prawn culture technology and the nitrite concentration is continuously increased are solved.

The invention relates to the technical field of fermentation industrial wastewater treatment, and particularly discloses a white spirit production wastewater treatment process and system. The system comprises a pretreatment unit, an anaerobic treatment unit, a denitrification unit, a multi-stage biochemical treatment unit, an advanced treatment unit, a sludge treatment unit and a backwashing water recovery unit which are connected through a corrosion-resistant pipeline, a variable frequency pump body and a control valve group. The method comprises the following steps: homogenizing water quality and water quantity through an adjusting tank, adjusting the pH value through a neutralizing tank, pretreating through a microfilter and an air flotation device, sequentially feeding into a hydrolysis acidification tank and a two-stage UASB reactor to degrade organic matters, reducing nitrogen concentration through a denitrification unit, deeply degrading pollutants through a multi-stage biochemical system, and finally realizing up-to-standard discharge through magnetic coagulation phosphorus removal and decoloration treatment. The sludge is subjected to resource utilization after being concentrated and dehydrated, and backwashing water and filtrate are recycled and retreated. The system is high in treatment efficiency, low in operation cost, high in resource recovery rate, stable in effluent and suitable for wastewater treatment of various white spirit production enterprises.

Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

The invention discloses a nicotinic acid immobilized enzyme conversion method, which comprises the following steps: taking wet thalli containing nitrilase, preparing a flocculation clear liquid, passing the flocculation clear liquid through an ultrafiltration membrane, carrying out embedding immobilization to obtain embedded particles not greater than 2mM, and after the embedded particles are hardened, separating and purifying the obtained conversion liquid by adopting a membrane separation method to obtain the nicotinic acid immobilized enzyme. Sequentially carrying out microfiltration, activated carbon decoloration, ultrafiltration, nanofiltration decoloration, acidification crystallization and centrifugation to finally obtain a nicotinic acid finished product. The immobilized enzyme prepared by the method disclosed by the invention can stably exist in nitrile substances, can be repeatedly used for many times, and is high in stability and low in enzyme activity loss rate, and a separated and purified product is free of impurities and high in yield.

The invention relates to the technical field of precipitation and sludge dewatering, in particular to a land-saving integrated treatment method for deep purification and sludge dewatering. When low-valence ions are removed as a treatment target, the device comprises a pretreatment unit, a biological enhancement precipitation and dehydration integrated tank, a filter tank, a microfiltration or ultrafiltration unit, a nanofiltration or reverse osmosis unit, a clean water tank, a drainage tank, a concentration tank, a pump room and related auxiliary pipelines, when low-valence ions and frequently-appearing organic pollutants are removed as a treatment target, the device comprises a pretreatment unit, a biological enhancement precipitation and dehydration integrated tank, an ozone contact tank, an activated carbon adsorption tank, a filter tank, a microfiltration or ultrafiltration unit, a nanofiltration or reverse osmosis unit, a clean water tank, a drainage tank, a concentration tank, a pump room and related auxiliary pipelines. The deep purification sludge dewatering land-saving integrated treatment method can integrate the processes of efficient precipitation, sludge dewatering, biological enhancement and land-saving integration, so as to realize deep purification of water quality and cyclic utilization of resources.

The invention discloses a reaction kettle parameter self-adaptive adjusting method based on a physical and biological double-cutting one-step method. The method comprises the following steps: in a physical cutting and biological enzymolysis synergistic reaction stage, establishing a reaction kettle parameter dynamic optimization model, and carrying out synergistic tuning on a first reaction parameter of a reaction kettle according to the reaction kettle parameter dynamic optimization model; in the first ultrafiltration separation stage, a pressure difference PH coupling control algorithm is constructed and used for adjusting a second reaction parameter of the reaction kettle in real time; in a second ultrafiltration refining stage, dynamically adjusting a third reaction parameter according to a preset multi-objective optimization model; in the microfiltration purification stage, a fourth reaction parameter is adjusted according to the product particle size distribution corresponding to the reaction kettle; in the nanofiltration concentration stage, a fifth reaction parameter is cooperatively adjusted based on an ionic strength feedback mechanism; obtaining the power efficiency, the membrane flux attenuation rate and the product yield index of the reaction kettle, and completing the self-adaptive adjustment of the parameters of the reaction kettle.

The utility model discloses a wine brewing wastewater treatment system. The wine brewing wastewater treatment system is composed of a grating filtering device, a microfiltration filtering device, an adjusting tank, an advanced air flotation device, a reaction tank, a primary sedimentation tank, an MCR tank, an advanced anaerobic reactor, a first-stage A tank, a first-stage O tank, a second-stage A tank, a second-stage O tank, an MBR tank, a catalytic electrolysis denitrification device, a coagulating sedimentation device and a precision filtering device. The brewing wastewater is filtered by a grating to separate larger particles in the brewing wastewater from the wastewater, the wastewater enters a regulating tank after passing through a microfiltration and separation device, then enters a reaction tank for dosing reaction after removing solid particles in the wastewater by an advanced air flotation device, and is precipitated by a primary precipitation tank, and the effluent enters an MCR tank to further remove macromolecular pollutants in the wastewater. The effluent enters an advanced anaerobic reactor for anaerobic biochemical treatment, the effluent enters an MBR device after being subjected to secondary AO treatment, the effluent is treated by the MBR device, and the effluent is discharged after reaching the standard after being subjected to catalytic electrolysis and coagulating sedimentation.

The invention relates to the technical field of food, in particular to a preparation method of low-sensitization hydrolyzed whey protein nanoscale microcapsule powder, which comprises the following steps: S1, preparing a whey protein solution, performing microfiltration, and treating with cysteine hydrochloride; s2, trypsin hydrolysis; s3, inactivating and cooling; s4, adding chymotrypsin, and synchronously applying ultrasonic waves; s5, bromelain is added, ultrafiltration is performed, and peptide fragments are collected; s6, preparing a capsule wall solution; s7, adopting a micro-fluidic chip to form a nano emulsion; s8, the mixture is added into a CaCl2 solution to be stirred, multi-layer self-assembly is conducted, and micro-capsule particles are obtained; and S9, carrying out ultrahigh-pressure micro-jet homogenization, vacuum freeze drying and low-temperature plasma assisted grinding on the micro-capsule particles to obtain the nano-scale micro-capsule powder. The stability of active ingredients is maintained through multi-enzyme cooperation and ultrasonic-assisted efficient elimination of allergens, multi-layer nano-embedding under microfluidic control and low-temperature plasma grinding, and the limitations of low single enzymolysis efficiency, insufficient embedding rate and thermal denaturation risk in the prior art are overcome.

The invention discloses a method for extracting, separating and purifying plant active ingredients, and belongs to the technical field of plant extraction.The method comprises the following steps that an MOF-enzyme nanocomposite is prepared, specifically, ZIF-8 nanoparticles are dispersed in an enzyme solution, dopamine-polyethylene glycol is added for a cross-linking reaction, and the MOF-enzyme nanocomposite is obtained through ultrafiltration and centrifugation; carrying out enzymolysis treatment, namely mixing the pretreated plant material with the complex, and carrying out enzymolysis; extracting: after enzymolysis, adjusting the pH value of the system to be neutral; and separation and purification: carrying out plate frame filtration and microfiltration to remove impurities, carrying out macroporous adsorption resin column chromatography, carrying out ethanol gradient elution, and carrying out concentration, crystallization and vacuum drying to obtain the target active component. According to the method, the enzymolysis efficiency and the component stability can be synchronously improved, and the enzyme utilization rate is improved, so that the technical problems that in traditional free enzyme extraction, the contact efficiency of enzyme and plant cell walls is low, and the enzyme is easily influenced by pH and temperature to be inactivated are effectively solved.

The application discloses a kind of intelligent switching water sample pretreatment system and method, system includes: combined sand filter sample barrel, for collecting water sample to be pretreated, initial water sample turbidity value acquisition is carried out, while water sample preliminary pre-filtering treatment is carried out;Positioning floating sampling device, for fixing to take supernatant after water sample settlement;Microfiltration module, for the microfiltration of ultrahigh turbidity water sample;Sample water transfer pump, for transmitting supernatant in combined sand filter sample barrel to microfiltration module;Sample collection barrel, for collecting and detecting water sample quantity after processing, for instrument or laboratory personnel to sample analysis;Valve assembly, by valve opening and closing, water sample is switched different pretreatment mode;Cleaning assembly, including rotary high-pressure spray head and self-heating ultrasonic cleaning device, for cleaning system after pretreatment internal residual adherend.

The invention discloses a method for extracting nutrient substances from sea cucumber processing wastewater and application, and belongs to the technical field of sea cucumber processing wastewater extraction. According to the method, the sea cucumber processing wastewater is used as a raw material, nutrient substances in the wastewater are extracted, the processing wastewater is pretreated by adopting an air floatation-microfiltration coupling technology, suspended solids and grease in the wastewater can be effectively removed, and heat-sensitive substances are reserved; meanwhile, a pH response type flocculating agent is adopted for removing heavy metal in a high-selectivity mode, meanwhile, loss of nutrient substances including protein, polysaccharide and saponin is avoided, after pretreatment is completed, a staged enzymolysis technology, a membrane separation-adsorption combined technology, a vacuum thin film evaporation technology and a programmed cooling crystallization technology are cooperated, protein, polysaccharide and saponin can be effectively separated and extracted, and the quality of the product is improved. The recovery rate, the purity and the product performance of each nutrient substance are remarkably improved, and technical support is provided for reutilization of nutrient components in sea cucumber processing wastewater.

The invention discloses a production process of earthworm protein powder, and aims to solve the problems of poor product quality and activity loss caused by no standard raw materials, non-standard hydrolysis parameters, more separated and filtered impurities, improper drying control and no storage control in the existing process. The process comprises the following steps: selecting fresh red frozen eisenia fetida; adding 2-3 times of reverse osmosis purified water, homogenizing and crushing for more than or equal to 1 hour until the yogurt is formed, and self-melting at 37 DEG C for more than or equal to 10 hours; taking supernate and using a tubular separator (controlling the flow rate and cleaning a rotary drum every 30 minutes); 2-3 times of purified water is added into the centrifugate, a membrane is washed regularly through microfiltration, the temperature is controlled through ultrafiltration, and residues are washed away through the purified water after alkali washing; drying at the temperature less than or equal to 50 DEG C (pre-cooling at-22 DEG C, raising 5 DEG C every 3 hours, and recording the temperature regularly); and airing the powder, performing ultraviolet sterilization for more than or equal to 2 hours, screening by 80-120 meshes, packaging, and storing in a finished product warehouse with controlled temperature and humidity (5-25 DEG C, 40-60%) and sterilization for 1 hour every day. The process improves the protein extraction rate, purity and activity retention rate, ensures the product safety and stability, and is suitable for industrial production.

The utility model discloses a zero-discharge treatment system for circulating cooling water. The zero-discharge treatment system comprises a hardness removal device, a first silicon removal device, a second silicon removal device, a microfiltration device, a reverse osmosis device and an evaporation device, the first silicon removal device is connected with the hardness removal device; the microfiltration device and the reverse osmosis device are sequentially connected with the first silicon removal device; the second silicon removal device is connected with the reverse osmosis device and is used for receiving reverse osmosis concentrated water and carrying out deep silicon removal treatment; and the evaporation device is connected with the second silicon removal device. According to the circulating cooling water zero-discharge treatment system disclosed by the utility model, circulating cooling water is treated through the hardness removal device, the first silicon removal device, the microfiltration device, the reverse osmosis device, the second silicon removal device and the evaporation device, so that pollutants such as calcium, magnesium, silicon, COD (Chemical Oxygen Demand) and phosphate in the circulating cooling water are removed to the maximum extent; and high-quality fresh water is recycled through a concentration, evaporation and crystallization technology, so that zero discharge of circulating cooling water is realized.