252results about "Protein composition from milk" patented technology

The invention discloses a protein separation method and related application thereof, and relates to the technical field of dairy product processing, the method comprises the following steps: heating a whey solution of which the pH value is adjusted to 3.5-4.2 by a citric acid solution, carrying out microfiltration on the heated product, carrying out beta-lactoglobulin and / or alpha-lactalbumin separation on permeate and trapped fluid obtained by microfiltration, the method is based on the membrane filtration technology, high-purity separation of beta-lactoglobulin and alpha-lactalbumin is achieved, the purity can reach 70%-90% or above, the method is compatible with whey from multiple sources, equipment is simple, the method has the advantages of being low in separation cost, high in efficiency, easy to implement, large in flux and the like, and a way is provided for large-scale industrial production of beta-lactoglobulin and alpha-lactalbumin.

The invention discloses a method for enriching whey protein main monomers from cow milk. According to the method, whey protein subjected to membrane separation is taken as a raw material, interaction between main monomers of the whey protein and other milk proteins is weakened by adopting a pH conversion technology and an ultrasonic technology, mineral substances, lactose, bovine serum albumin and residual casein are removed through ion exchange chromatography, the main monomers of the whey protein are enriched, and the high-purity whey protein is obtained. And drying the alpha-lactalbumin and the beta-lactoglobulin to obtain a milk protein product rich in two main monomers of the whey protein, and the total content of the two monomers is up to 85% or above. According to the technology, cow milk serves as a raw material, efficient enrichment of two main monomers of whey protein can be achieved only through membrane separation, pH conversion, ultrasonic assistance and ion exchange chromatography, only pH needs to be adjusted in the separation process, other chemical reagents are not added, and the process is environmentally friendly, simple, easy to implement and suitable for large-scale processing; the prepared milk protein base material rich in the whey protein main monomer can be used as a main material and a base material of infant food, and is high in nutritional value and wide in application prospect.

The present disclosure relates to novel methods of assembling compositions comprising casein micelles; recombinant casein micelles, and their uses in the production of food products.

The invention discloses bovine lactoferrin freeze-dried powder and a preparation method thereof, and belongs to the technical field of protein freeze-drying. The preparation method of the bovine lactoferrin freeze-dried powder comprises the following steps: adding a histidine buffer solution into a bovine lactoferrin solution with the mass concentration of 30-40%, and mixing to obtain a mixed solution; adding a freeze-drying auxiliary material accounting for 0.25-7.0% of the volume of the mixed solution into the mixed solution to obtain a bovine lactoferrin freeze-drying solution; performing spray freezing and freeze drying on the bovine lactoferrin freeze-dried liquid to obtain bovine lactoferrin freeze-dried powder; the freeze-drying auxiliary materials comprise a surfactant, amino acid, D-chiro-inositol and calcium chloride. Compared with the traditional inositol, the D-chiral inositol in the freeze-dried auxiliary material can more effectively replace water molecules, maintain the natural conformation of protein and prevent the bovine lactoferrin structure from collapsing. The calcium chloride in the freeze-drying auxiliary material can stabilize the iron binding area of the bovine lactoferrin in the freeze-drying process, and the biological activity of the bovine lactoferrin after freeze-drying is guaranteed.

The present invention relates to a nutrition bar comprising a protein and extraneous β-lactoglobulin. The invention further relates to a process for the preparation of such nutrition bar. The invention further relates to the use of a β-lactoglobulin to modulate the hardness, firmness and / or cohesiveness of a nutrition bar.

A method for producing a spongy starch-free dairy snack, which comprises the steps of: preparing a food paste that includes 75-87% by weight OF acid casein and 13-25% by weight of powdered cheese; extruding the food paste at a pressure and temperature that enables the powdered cheese particles to fuse with the acid casein particles; cutting the string of extruded paste; allowing the pieces of extruded paste to expand; and drying the pieces of expanded paste until they contain 6-13% by weight of moisture, to obtain the spongy snack. The method also includes a step of hydrating the food paste with alkaline water before or during the step of extrusion. The obtained snack has a density of 75-150 g / L and a dry protein content of 80-89% by weight.

The invention discloses a protective agent as well as a preparation method and application thereof. The protective agent is prepared from inulin, fructo-oligosaccharide and erythritol. According to the protective agent provided by the invention, the inulin, the fructo-oligosaccharide and the erythritol are compounded to form a mixed protective agent, so that the heat resistance of functional protein in the bovine colostrum processing process can be improved, and the protective agent has a good protective effect on immune globulin (Ig-G) and lactoferrin (LF) in the processing process. Moreover, the three components all have probiotic effects, so that the development of high-value bovine coloctrum functional products is facilitated, and the comprehensive benefits of the bovine coloctrum are improved.

The invention relates to the field of food, in particular to colostrum basic protein as well as a preparation method and application thereof. The colostrum basic protein of the present invention can increase bone mass, increase bone density, promote skeletal development, promote skeletal regeneration or repair, promote calcium absorption, promote bone collagen production, and / or prevent and / or treat bone related diseases (such as osteoporosis, osteoporosis, rickets, osteoarthrosis).

The present application relates to the production of novel beta-lactoglobulin preparations and related methods, uses and food products. The present invention relates to novel methods of producing isolated beta-lactoglobulin compositions and / or compositions containing crystalline beta-lactoglobulin. The present invention also relates to novel beta-lactoglobulin compositions, uses of these compositions and food products comprising these compositions.

The utility model relates to the technical field of cow milk protein separation and purification, and provides a cow milk protein component synchronous separation system. Comprising a milk bin, a fat separator, a first cation exchange device, a first membrane filtration module, a casein solution treatment branch, a second membrane filtration module, a second cation exchange device, a first anion exchange device, a second anion exchange device, a purification and sterilization treatment branch and a product production line. Membrane filtration and a chromatographic technology are combined, and different active proteins are separated by using the molecular weight, the charged property, the hydrophobic interaction and the like of the proteins. Main active protein in milk can be separated, beta-casein, alpha-lactalbumin, immune globulin and lactoferrin can be sequentially separated and purified from raw milk, drying or sterile filling is carried out, different products are prepared while natural nutritional ingredients are reserved, and in addition, the product quality is improved. The separated milk can still be processed into sterilized milk or milk beverages.

The present invention relates to a method to increase the physical stability of a β-lactoglobulin and to a β-lactoglobulin with increased physical stability. The present invention further relates to the use of an extraneous β-lactoglobulin to enhance the physical stability of a composition comprising a protein and the extraneous β-lactoglobulin.

The invention discloses camel milk protein-polysaccharide composite nanoparticles and a preparation method thereof, and belongs to the technical field of preparation of natural fermentation composite particles. The preparation method comprises the following steps: (1) inoculating phytobacterium plantarum into camel milk for fermentation, centrifuging, and collecting supernatant; and (2) carrying out ultrasonic treatment on the supernatant under an ice-water bath condition, adding absolute ethyl alcohol to precipitate after the ultrasonic treatment is finished, standing, centrifuging, and dialyzing to remove impurities, so as to finally obtain the camel milk protein-polysaccharide composite nanoparticles. According to the preparation method, camel milk is taken as a fermentation substrate, fermentation is carried out by utilizing phytobacterium plantarum LR-1, and the natural polysaccharide-protein nanoparticles (PPNPs) are successfully prepared in combination with ultrasonic treatment. In the fermentation process, the metabolic activity of lactic acid bacteria promotes the combination of protein and polysaccharide, and the particle size, the stability and the oxidation resistance are improved; the ultrasonic treatment further optimizes the particle size and structure, and enhances the oil-water interface adsorption capacity.

The invention discloses a milk protein base material rich in beta-casein and a preparation method of the milk protein base material. The method comprises the following steps: firstly, obtaining a casein micelle trapped fluid through a membrane separation technology, and secondly, promoting casein micelles to be partially dissociated and partially precipitated by adopting a pH conversion combined ultrasonic technology, so that the interaction between a casein monomer and whey protein is destroyed; adjusting the pH value of the solution to be neutral, and dissolving the casein; the preparation method comprises the following steps: adding calcium chloride to promote the aggregation of the alpha-casein by utilizing the sensitivity of the alpha-casein to calcium ions, and intercepting the alpha-casein by utilizing a membrane separation technology to realize the enrichment of the beta-casein so as to prepare the milk protein base material rich in the beta-casein. Compared with the traditional column chromatography technology, the technology has the advantages that the efficient enrichment of beta-casein and the efficient separation of alpha-casein can be realized through the membrane separation and pH conversion combined ultrasonic technology; in the separation process, only pH needs to be adjusted, other auxiliaries are not needed, and the process is environmentally friendly, simple, easy to implement and suitable for large-scale processing; the prepared milk protein base material rich in beta-casein can be used as a main material and a base material of infant food, and is high in nutritional value and wide in application prospect.

The present disclosure provides systems, devices, and methods for obtaining fat- and protein- enriched milk from unenriched milk. As provided herein, the present disclosure encompasses a device, comprising a filtration unit for increasing the concentration of protein and fat in unenriched milk to obtain a human milk origin fortifier, as well as a UV measurement head device for measuring the fat and protein content of an enriched or unenriched milk sample.

The present invention relates generally to food products comprising proteins comprising sulfur-comprising amino acids, and to methods for producing such food products.

The present invention pertains to a new type of CMP-containing, high protein denatured whey protein compositions and to a method of producing them. The invention furthermore pertains to products containing the high protein denatured whey protein compositions, particularly high protein, acidified dairy products, and additional uses of the denatured whey protein compositions.

The invention relates to the field of liquid nutritional compositions having a high protein content. More particularly, the invention relates to a nutritional composition comprising between 14 and 30 g of proteins per 100 ml, wherein the proteins are hydrolysed. This composition has a complete mineral profile and a suitable energy content for use as the sole source of food for malnourished patients.

The invention relates to a process for dephosphorylation of skim milk, ultra-filtered milk (UF milk) or micellar casein isolate (MCI) comprising (i) acidification of a skim milk, UF milk or MCI preferably not lower than pH 6.0, preferably between 6.0 and 6.7, (ii) cooling the acidified skim milk, UF milk or MCI to the temperature between 0° C. and 15° C., (iii) adding gluconate and / or maleate to the cooled skim milk, UF milk or MCI and, (iv) washing the skim milk, UF milk or MCI, to remove phosphorus, thus dephosphorylating the skim milk, UF milk or MCI, preferably to an extent that the total phosphorous content of the skim milk, UF milk or MCI is reduced with at least 20%, preferably 20-40%, more preferably 30-40% compared to the material provided to step (i). The invention also relates to dephosphorylated MPC, MPI or MCI obtainable by the process of the invention, and to a liquid heat-sterilized enteral nutritional composition comprising 2.0-3.0 kcal / ml wherein 16-35 en % is provided by protein, the combination of caloric content and relative protein caloric content selected such that there is 10-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein in the composition, wherein the protein comprises micellar casein (MC), whey protein (WP) and optionally caseinate (CAS), wherein there is at least 70 wt % MC and less than 15 wt % WP, based on total protein content, and wherein the composition has a total amount of phosphorous less than 192 mg / 100 ml and / or 30-80 mg / 100 kcal, preferably at least 30-80 mg / 100 kcal, most preferably less than 192 mg / 100 ml and 30-80 mg / 100 kcal.

The present disclosure provides devices and methods for obtaining protein-enriched fractions from human milk. As provided herein, the present disclosure encompasses a device, comprising a delipidation unit for reducing lipid content in the human or animal milk to obtain delipidated milk and a protein-enrichment unit for increasing a protein concentration of the delipidated milk to obtain the protein-enriched fraction.

The present invention relates to a method for producing a lactoferrin-containing product by performing cation exchange with milk having a lactoferrin concentration of at least 4.0 per gram of protein. The invention also relates to a lactoferrin-containing product containing at least 96 wt% of lactoferrin and less than 0.60 wt% of immunoglobulin (IgG + IgA + IgM), based on the total protein.

The present invention relates to a method for production of recombinant casein, wherein the method comprises the steps of: (a) adding a microbial strain to a first bioreactor, wherein said microbial strain has the capacity to express casein, and said microbial strain comprises means for deactivation of cell division; (b) culturing said microbial strain in the first bioreactor, wherein the cell division is active, thereby forming a continuously growing biomass comprising cells of said microbial strain; (c) deactivating cell division; (d) inducing expression of casein; (e) after sufficient expression of casein, lysing said cells, thereby forming a lysis broth, and separating said lysis broth so that casein can be separated into a casein containing phase; (f) optionally treating the casein containing phase to induce formation of casein micelles; (g) optionally further purifying and / or treating the casein micelles, and (h) optionally producing caseinate. In other aspects, the invention also relates to a bioreactor system, a recombinant casein, and products including a recombinant casein.

The invention relates to a process for obtaining a milk extracellular vesicle (EV)- enriched product from whey by mixing whey with a PEG solution providing a whey- PEG mixture; and centrifuging the whey-PEG mixture providing a pellet enriched in milk EV.

The present invention pertains to a method of producing whey lipid powders having both a high content of bioactive components such as native IgG and native lactoferrin and at the same time a sufficient microbial quality that makes the powders useful for production of e.g. infant formula powders by dry-blending. The invention also pertains to a whey-derived powder enriched with respect to whey lipid and to processes of using the whey-derived powder and to nutritional products containing the whey-derived powder.

The present invention pertains to a method of producing beta-casein-containing compositions and products obtainable by such methods. More particularly, the invention pertains to a method of producing beta-casein compositions using controlled microfiltration first at a temperature higher than 20 degrees C, followed by cooling of the retentate to 0-15 degrees C and a second microfiltration of the cooled composition resulting in beta-casein containing permeate.

Disclosed herein is a process for producing a lactoferrin powder. The process comprises extracting lactoferrin from a native lactoferrin-containing substance and concentrating the extracted lactoferrin to produce a liquid concentrate in which the extracted lactoferrin has an improved stability. The liquid concentrate is then pasteurised by heating to a temperature and for a time effective to do so, after which the pasteurized liquid concentrate is dried under non-denaturing conditions to produce a lactoferrin powder in which substantially all of the lactoferrin is native lactoferrin.

The present invention relates to a process for the dephosphorylation of skimmed milk, ultra-filtered milk (UF milk) or micellar casein isolate (MCI) comprising (i) acidifying the skimmed milk, UF milk or MCI, preferably not below pH 6.0, preferably between 6.0 and 6.7, (ii) cooling the acidified skimmed milk, UF milk or MCI to a temperature between 0 DEG C and 15 DEG C, (iii) adding gluconate and / or maleate to the cooled skimmed milk, UF milk or MCI, and (iv) washing the skimmed milk, UF milk or MCI to remove phosphorus, thereby dephosphorylating the skimmed milk, UF milk or MCI, preferably to the extent that the total phosphorus content of the skimmed milk, UF milk or MCI is reduced by at least 20%, preferably 20-40%, more preferably 30-40%, compared to the material provided to step (i). The invention also relates to a dephosphorylated MPC, MPI or MCI obtainable by the process of the invention and to a liquid enteral nutritional composition comprising 2.0-3.0 kcal / ml of heat sterilization wherein 16 en%-35 en% is provided by a protein, the combination of the calorie content and the relative protein calorie content being selected such that 10-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein is present in the composition, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein. Wherein the protein comprises micellar casein (MC), whey protein (WP) and optionally caseinate (CAS) wherein at least 70 wt% MC and less than 15 wt% WP are present, based on the total protein content, and wherein the composition has a total amount of phosphorus of less than 192 mg / 100 ml and / or 30-80 mg / 100 kcal, preferably at least 30-80 mg / 100 kcal, most preferably less than 192 mg / 100 ml and 30-80 mg / 100 kcal.

A recombinant protein for use in the treatment of a proliferative disease or disorder in a subject is provided, wherein the recombinant protein comprises at least one amino acid substitution or deletion relative to a corresponding native protein, and wherein the recombinant protein is not a hormone, an enzyme or an antibody.