105 results about "Alpha-lactalbumin" patented technology

The invention discloses a milk allergen test plate, which belongs to the gold immunochromatography detection. According to the milk allergen test plate disclosed by the invention, two ends of a PVC (polrvinyl chloride) base plate are respectively provided with a to-be-tested sample zone and an absorption zone. Colloidal gold mark antigens prepared by respectively marking colloidal gold into casein, beta lactoglobulin and alpha lactalbumin and mixing are orderly arranged between the sample zone to be tested and the absorption zone, and a nitrocellulose membrane is respectively provided with a detection zone coated with mixed milk allergen and a quality control zone coated with anti-beta lactoglobulin antibodies. In detection, color lines are formed in the detection zone and the quality contol zone when an immune complex is formed by specific milk antibodies contained in samples. If the samples do not contain specific milk antibodies, the detection zone does not display color, and only one color line is formed in the quality control zone. The milk allergen test plate disclosed by the invention with the design has the advantages of strong pertinence to the allergen detection, simplicity in operation, low cost and high sensitivity and the like, and can prevent the phenomenon of missed diagnosis in the single antibody detection. The milk allergen test plate is applied for rapidly screening patient allergic to milk, and is especially suitable for being used by primary medical treatment units.

The invention discloses infant formula goat milk powder and a preparation method thereof. Alpha-lactalbumin and hydrolytic lactalbumin peptide, malt sugar (corn syrup), glucose, pure colostrum powder, lactoferrin, living bifidobacterium and the like are added in the infant formula goat milk powder. The method comprises the steps of: obtaining goat milk, performing pasteurization (85 DEG C), cutting material mixing tanks with high speed (the material in the tank includes malt sugar, glucose, decavitamin, mineral nutrient, hydrolytic lactalbumin peptide and plant oil), using a homogenizer, performing pasteurization, vacuum concentration and spray drying, cooling a fluid bed (adding the lactoferrin, the pure colostrum powder IgG and the plant oil), mixing the added materials (adding the lactoferrin, the pure colostrum powder IgG and the living bifidobacterium), and canning. The infant formula goat milk powder is easy to digest and absorb, and muting sensitive; and the formula powder is more humanized, and can enhance organism immunity.

The present invention provides compositions and formulations comprising alpha-lactalbumin and a carbohydrate of high glycemic index. This invention also provides methods of treating pain, pre-menstrual syndrome, and symptoms associated with the pre-menstrual period and menopause.

The invention discloses a method for quantitatively detecting allergen alpha-lactalbumin based on quantum dot fluorescence and application of the allergen alpha-lactalbumin. The method comprises the following steps of: preparing a monoclonal antibody from alpha-lactalbumin as active immunization BALB / c mice; carrying out conjugation labeling on the alpha-lactalbumin monoclonal antibody by using a fluorescent quantum dot; forming an immunofluorescence complex by adopting a competitive immunosorbent assay; then detecting a fluorescent signal under a full-wavelength multifunctional ELIASA (Enzyme-Linked Immunosorbent Assay Apparatus); and quantitatively detecting the allergen alpha-lactalbumin in the food by establishing a standard curve. The method constructed by the invention can be widely applied to detection of relevant allergens in various powders and liquid milk products, has the characteristics of quickness, accuracy, high sensitivity, favorable repeatability, excellent specificity and the like, provides an effective means for high-throughput detection of relevant allergens in various foods as well as has a favorable popularization and application prospect.

The invention relates to the field of analytical chemistry and discloses a method and kit for quantitative detection of content of human alpha-lactalbumin. According to the method disclosed by the invention, a specific polypeptide sequence CELSQLLK obtained after enzymolysis of the human alpha-lactalbumin is utilized to design the sequences of an isotope-labeled specific peptide and an isotope-labeled internal standard substance, a brand new accurate quantitative detection method is provided on the basis of an internal standard method to perform the quantitative detection of the human alpha-lactalbumin, and the accuracy, precision and sensitivity are relatively high.

The invention provides a fermented milk which is excellent in hardness and flavor and a method of producing the same. The method of producing a fermented milk by using a yogurt mix in which 0.3% by weight or more alpha-lactalbumin is added to a starting milk mixture, a yogurt mix containing a whey protein concentrate in which alpha-lactalbumin is contained in an amount of 60% by weight or more based on the protein, a yogurt mix in which 0.4% by weight or more beta-lactoglobulin is added to a starting milk mixture, or a yogurt mix containing a whey protein concentrate in which beta-lactoglobulin is contained in an amount of 65% by weight or more based on the protein.

The invention relates to an enzymatic hydrolysis alpha-lactalbumin polypeptide and an enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle and preparation thereof. Alpha-lactalbumin with a certain purity as a raw material is used for enzymatic hydrolysis in the presence of a particular enzyme to obtain the ideal enzymatic hydrolysis alpha-lactalbumin polypeptide, the enzymatic hydrolysis alpha-lactalbumin polypeptide can be used for preparing the stable particle size enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle, the raw material is low in cost and easy to obtain, and the preparation process is simple; and the micelle is small in particle size, good in dispersion, good in biocompatibility and high in stability, the micelle can be used for embedding of hydrophobic materials, the micelle as a carrier can be made into a supported enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle, and the supported enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle has the advantages of small particle size, good stability, high encapsulation efficiency and non-toxicity.

The invention provides a low-sensitivity glycosylation whey protein hydrolysate and a preparation method thereof. In the preparation method, an enzymolysis method is combined with a glycosylation method, and the content of allergen can be reduced to the greatest degree, wherein enzymes adopted in the enzymolysis method include alkaline protease, neutral protease, papain, trypsin, flavourzyme, protease A and protease M; the sugar adopted in the glycosylation method is one of maltooligosaccharide, glucose and phosphoryl oligosaccharide or a mixture of more. By adopting the method provided by the invention, the antigenicity inhibition ratio on whey protein can be effectively reduced, wherein the antigenicity inhibition ratio on alpha-lactalbumin (alpha-LA) reaches 5%, and the antigenicity inhibition ratio on beta-lactoglobulin (beta-LG) reaches 2%.

The invention discloses an immunodetection kit for detecting adulterated cow milk in camel milk and an application thereof, wherein alpha-lactalbumin and alphas1-casein in camel milk are used as characteristic protein of the camel milk, and beta-lactoglobulin and kappa casein in cow milk are used as characteristic protein of the cow milk. After the camel milk characteristic protein is separated and purified, the polyclonal antibody and the monoclonal antibody prepared by the commercialized cow milk characteristic protein and the self-made camel milk characteristic protein can be applied to ELISA detection. The kit mainly adopts an indirect ELISA method to qualitatively or quantitatively detect the content of camel-derived alpha-lactalbumin, alphas1-casein or bovine-derived beta-lactoglobulin and kappa-casein in camel milk, the sample pretreatment process is simple, and after a camel milk sample is repeatedly centrifuged to obtain a milk sample, the content can be directly measured after diluted by diluent, and a large number of samples can be simultaneously detected. The determination method is accurate, rapid and convenient, and has wide practicability and development value in thefield of camel milk adulteration monitoring.

The present invention relates to a pharmaceutical composition comprising mono- meric alpha-lactalbumin complex, preferably LAC, which is an active complex of alpha-lactalbumin and a fatty acid or lipid with selective cytotoxic activity. The composition of the invention comprises insignificant amounts of oligomeric / multi- meric alpha-lactalbumin complex, preferably LAC. Based on the selective cyto- toxicity of the alpha-lactalbumin complex, preferably LAC composition such compositions are suitable for use in the manufacture of medicaments for use in therapy. Medicaments, comprising monomeric LAC are for use in the treatment of bacterial and viral infections and in particular cancer due to the selective cytotoxic activity. The application further relates to methods of producing a composition comprising monomeric alpha-lactalbumin complex, preferably LAC with cytotoxic activity.

An infant formula having a relatively high content of triglycerides having palmitic acid in the sn-2 position. The formula may include oligofructose. The formula may also include at least one omega 6 fatty acid and at least one omega 3 fatty acid. The formula may also have a relatively low protein content and an alpha-lactalbumin content similar to human milk The invention also includes a method for improving the stool consistency, increasing bifidobacteria in the colon, reducing potentially pathogenic bacteria in the colon and reducing calcium soaps in the stool of a formula-fed infant.