244 results about "Internal standard" patented technology

The invention relates to the technical field of tobacco leaf element analysis, in particular to a detection method for volatile compounds in tobacco leaves and a clustering analysis method for the tobacco leaves. The method for detecting the volatile compounds in the tobacco leaves comprises the following steps: drying and pulverizing tobacco leaves to be detected to prepare sample powder of the tobacco leaves to be detected; extracting the mixed solution of the tobacco leaf sample powder to be detected and the internal standard solution by adopting a headspace solid-phase microextraction method to prepare an extracting solution; carrying out gas chromatography-mass spectrometry detection on the extract liquor; wherein an extraction head used by the headspace solid-phase microextraction method is a divinylbenzene / carbon molecular sieve / polydimethylsiloxane composite coating extraction head, and the parameters of the headspace solid-phase microextraction method are as follows: the extraction temperature is 75-85 DEG C, the extraction time is 45-55 minutes, the desorption time is 4-6 minutes, and the equilibrium time is 3-7 minutes. The method for detecting the volatile compounds in the tobacco leaves, provided by the invention, can effectively separate and analyze the volatile components, and is simple and efficient.

The application provides a pretreatment and detection method of tetrabromobisphenol A and ten common derivatives thereof in sludge, which comprises the following steps: (1) sample pretreatment: after mixing the frozen and dried sludge sample and the isotopic internal standard, oscillation extraction is carried out, and the extraction liquid is obtained after centrifugation by a centrifuge; the extraction liquid is purified by solid phase extraction to obtain a sample to be detected; (2) the sample to be detected obtained in the step (1) is detected by high performance liquid chromatography-tandem mass spectrometry; (3) the content of tetrabromobisphenol A and ten common derivatives thereof in the sample is calculated according to the internal standard method. The application provides a rapid and sensitive analysis and quantitative method of tetrabromobisphenol A and ten common derivatives thereof in sludge samples.

The invention provides a method for detecting the content of elements in carbonized resin. The method comprises the steps of resin pretreatment, instrument tuning, standard curve drawing, sample detection and the like. The method comprises the following steps: firstly, transferring a certain amount of dry carbonized resin into a cleaned PFA digestion tank, adding high-purity mixed acid, setting a segmented heating program to start microwave digestion, cooling to room temperature after digestion is completed, adding a proper amount of ultrapure water to dilute a digestion solution, and adding an internal standard solution and a chemical additive to obtain a to-be-detected sample solution; preparing a multi-element mixed standard solution according to the sample element types, adding the standard solution into the sample solution, and measuring the signal intensity ratio R of the to-be-measured element to the internal standard in each solution; and drawing a standard curve by taking the labeling concentration as a horizontal coordinate and R as a vertical coordinate, correcting the standard curve, and detecting the sample to be detected. The method is used for detecting the content of elements in carbonized resin, the carbonized resin is digested through microwaves, and the obtained solution is clear and can be directly subjected to ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometer) detection.

A method for optimizing at least one parameter setting of at least one mass spectrometry device (110) operating at unit resolution is disclosed. The method comprises the following steps:a) determining at least one analyte detection window for detecting an analyte of interest with the mass spectrometry device (110), wherein the analyte detection window is defined by a central mass to charge ratio value of the analyte and a predefined width, wherein the central mass to charge ratio value of the analyte is set to a theoretical mass to charge ratio value of the analyte of interest having more than one decimal place and / or a mass to charge ratio value of the analyte of interest determined by a high resolution mass spectrometry measurement having more than one decimal place;b) determining at least one internal standard detection window for detecting an internal standard substance with the mass spectrometry device (110), wherein the internal standard detection window is defined by a central mass to charge ratio value of the internal standard substance and the pre-defined width, wherein the central mass to charge ratio value of the internal standard substance is set to a mass to charge ratio value of the internal standard substance calculated relative to the analyte of interest and having more than one decimal place and / or to a mass to charge ratio value of the internal standard substance determined by a high resolution mass spectrometry measurement having more than one decimal place.

The present application relates to the field of analytical chemistry, in particular to a method for detecting ten tobacco alkaloids in soil, which uses sodium hydroxide solution and acetonitrile to extract alkaloids in a soil sample, and uses ultra-high performance liquid chromatography-triple quadrupole mass spectrometry to analyze and detect nicotine, nornicotine, myosmine, pseudotropine, anatabine, anabasine, 2,3'-bipyridine, noranatabine, N-methyl pseudotropine and nornicotine, and uses an internal standard method for quantitative analysis. The present application uses sodium hydroxide solution as an alkaloid eluent and acetonitrile as an extractant, which reduces the amount of solvent used, optimizes the operation process, uses sodium chloride to separate the organic phase and the aqueous phase, improves the sample pretreatment efficiency, uses ultra-high performance liquid chromatography-triple quadrupole mass spectrometry to target analysis of 10 tobacco alkaloids, greatly improves the detection sensitivity, and has strong quantitative ability in targeted analysis.

The application relates to the technical field of analytical chemistry detection, and specifically discloses a rare earth ion chromatographic online analysis and detection method and system, which adopts a chromatography-mass spectrometry combined system, realizes multi-scale time-frequency decomposition of signals by constructing an adaptive wavelet base function library, accurately identifies and classifies interference types in combination with a convolutional neural network and vacuum degree coupling analysis; a U-Net generator with an attention mechanism and a multi-scale discriminator are designed for signal repair aiming at repairable interference; a state transition model containing a mass-to-charge ratio database is established, empirical mode decomposition and adaptive filtering technology are used to realize dynamic calibration of a mass axis; quantitative accuracy is ensured through double internal standard correction and a triple verification mechanism; when unrepairable interference is detected, a hierarchical self-checking program is started, and fault diagnosis is carried out in combination with spectrum fingerprint analysis.

The invention belongs to the technical field of chemical screening, and particularly relates to a systematic screening method for rubber-related chemicals in a plasma sample, which comprises the following steps: S1, taking the plasma sample, adding a mixed extraction solvent, extracting, centrifugally layering, collecting supernate, repeatedly extracting, merging the supernate, concentrating, and freezing to obtain a rubber-related chemical sample; centrifuging again, adding an isotope internal standard, and carrying out data dependence acquisition in a positive and negative ion analysis mode by adopting liquid chromatography-tandem high-resolution mass spectrometry. According to the invention, the comprehensive coverage type monitoring of 1300-1500 RDCs in the plasma sample is realized. By means of combination of a positive and negative ion double analysis mode and data dependence acquisition, the detection range and the signal response stability of the RDCs with different polarities are effectively considered, and the technical problem that in the prior art, the composite effect generated by coexistence of multiple RDCs is ignored is solved.

The invention discloses a method for determining a quaternary ammonium salt pesticide in blood by using liquid chromatography-mass spectrometry, which comprises the following steps of: drawing a whole blood (plasma and serum) matrix labeling standard working curve through liquid chromatography-mass spectrometry analysis; obtaining peak areas of quantitative ion peaks of target objects containing paraquat, aquacide, diguat, chlormequat and mepiperidine with different concentrations and peak areas of quantitative ion peaks of internal standard substances, and further drawing a standard working curve; and performing liquid chromatography-mass spectrometry analysis on the whole blood sample under the same condition to obtain the peak area of a target substance quantitative ion peak and the peak area of an internal standard substance quantitative ion peak in the whole blood test solution, and calculating the content of each quaternary ammonium salt pesticide according to the standard working curve. The method can be used for simultaneously detecting the content of five quaternary ammonium salt pesticides in the whole blood of the poisoned patient simply, conveniently and quickly, and has the characteristics of simplicity, accuracy, good repeatability and high sensitivity.

The invention discloses a multi-element quantitative analysis method for laser ablation inductively coupled plasma mass spectrometry, and aims to solve the problems that the quantitative process of the existing LA-ICP-MS technology depends on a standard sample, the automation degree is low, and the existing LA-ICP-MS technology cannot adapt to complex multi-element signals. According to the method, full-automatic and multi-element (such as 9 main quantities and 30 trace elements) quantitative analysis is realized in the LA-ICP-MS field without an internal standard and an external standard, the analysis efficiency, the accuracy and the data comparability among different platforms are remarkably improved, and a breakthrough solution is particularly provided for application of element quantitative imaging and the like.

The invention discloses a multi-dimensional high-precision detection method and system for biotoxins in agricultural products, and the method comprises the steps: carrying out sterile collection and grading pretreatment on an agricultural product sample to obtain a homogenized sample; extracting the homogenized sample by adopting a mixed solvent system, adding an internal standard substance, carrying out salting-out layering, and collecting supernate; sequentially carrying out QuEChERS purification, multi-mechanism adsorption column purification and molecularly imprinted polymer specific enrichment on the supernate; carrying out dual-mode detection on the target toxin enrichment liquid, respectively carrying out quantitative detection by adopting a liquid chromatography-tandem mass spectrometry method, and carrying out screening detection by adopting an intelligent electrochemical sensing method; performing optimization processing on the dual-mode detection signal, and comprehensively judging a sample detection result; through integrated application of a triple purification mechanism, a dual-mode detection system, machine learning signal analysis and a full-chain quality control technology, efficient enrichment and deep interference removal of target toxins in a complex matrix can be realized, and both precision and applicability are considered.

The invention discloses a detection method of vitamin K1, and belongs to the technical field of biological detection. The problems that a vitamin K1 detection method is tedious in operation, prone to being influenced by a matrix, low in sensitivity and the like are solved. The detection method comprises the following steps: (1) preparing a standard substance intermediate working solution, an isotope internal standard substance solution and a standard curve working solution of the vitamin K1; (2) treating a serum sample and a standard curve working solution: adding a 2-nitroso pyridine solution to carry out derivatization reaction; and (3) detecting the vitamin K1 by using a high performance liquid chromatography-tandem mass spectrometry method. According to the method, the 2-nitroso pyridine is used as a derivatization reagent, the vitamin K1 and the 2-nitroso pyridine are subjected to a Diels-Alder reaction to generate a new six-membered heterocycle containing N and O, the 2-nitroso pyridine can efficiently and completely react with the vitamin K1 in only 10 minutes at room temperature, the detection steps are simplified, the sample pretreatment time is shortened, and the detection efficiency is improved.

This application provides a method for simultaneously detecting hydroxy acids and keto acids in a sample. The method includes the following steps: preparing a standard solution; performing ketone and carboxyl derivatization on the sample to be tested containing hydroxy acids and keto acids to obtain the sample to be tested; performing ketone and carboxyl derivatization on the standard solution to obtain a derivatized standard solution; and sequentially adding a ketone derivatization reagent and... 13 The method involves derivatization with a C6-labeled carboxyl derivatization reagent to obtain an isotope internal standard solution. This solution is then added to both the sample to be tested and the derivatized standard solution for LC-MS / MS analysis. The method described in this application achieves unified detection of paired hydroxy acids and keto acids by selectively derivatizing the ketone group followed by the carboxyl group. A sequential derivatization strategy is constructed based on the functional group differences between keto and hydroxy acids, significantly improving the stability, repeatability, and quantitative reliability of keto acid detection while enhancing overall detection sensitivity.

The invention relates to a method for rapidly determining the content of K.2. 2.2. 2 in an FDG card sleeve kit leacheate, and belongs to the technical field of radiopharmaceutical analysis. The method comprises the following steps: selecting a 1110 + / -5 cm <-1 > peak as a quantitative characteristic peak of K.2.2. 2, wherein the peak corresponds to symmetric stretching vibration of a C-O-C ether bond in a K.2.2. 2 molecule; selecting a 2253 + / -3cm <-1 > peak as an internal standard peak, wherein the peak corresponds to C = N triple bond stretching vibration of acetonitrile in the leacheate; preparing a K.2. 2.2. 2 gradient solution with the concentration of 0.05 to 5 mg / mL, and fixing the concentration of K2CO3 to be 0.5 to 2 mg / mL; the method comprises the following steps: acquiring a spectrum by adopting an ATR technology, setting the resolution ratio to be 4cm <-1 > and the scanning frequency to be 32, and establishing a K.2. 2.2. 2 linear model according to the ratio of A1110 / A2253; eliminating baseline drift by adopting a second derivative spectrum; and a PLS algorithm is adopted to correct trace interference of K2CO3.

This invention provides a method for the simultaneous and rapid detection of four oxidative stress-related biomarkers and its application. The detection method includes: (S1) pretreatment of samples using a protein precipitation extract to simultaneously extract four target compounds: 8-hydroxydeoxyguanosine, 8-isoprostaglandin F2β, 8-isoprostaglandin F2α, and acetyllysine, and preparing a test solution; the protein precipitation extract is a methanol-acetonitrile-perchloric acid mixed solution containing formic acid, wherein the volume ratio of methanol, acetonitrile, and perchloric acid is (8-10):(8-10):(0.5-1), and the volume concentration of formic acid is 0.1-0.5%; (S2) detection of the test solution and standard solution using liquid chromatography-tandem mass spectrometry, and matrix calibration using an internal standard method; plotting a standard curve with the peak area ratio of the target compound to the internal standard as the ordinate and the concentration as the abscissa; and calculating the concentration of the target compound in the test solution based on the standard curve.

This invention provides a precise quantitative sequencing method based on multi-level atomic structure internal standards, belonging to the field of quantitative metagenomic sequencing technology. The invention first constructs a multi-level atomic structure internal standard system containing at least two concentration levels of internal standard plasmids, forming multiple statistical response units within the same concentration level. After adding the internal standard plasmids to the nucleic acid of the sample, library construction and sequencing are performed. The sequencing signals of the target gene and statistical response units are statistically analyzed, sequencing response parameters are calculated, and consistency analysis is performed on multiple statistical response units within the same concentration level to determine the dynamic limit of detection (LOQ) and to determine the validity of the target gene quantification results. This invention can dynamically determine the LOQ under the current experimental conditions, improving the reliability of quantification results for low-abundance targets.

The application discloses a hydroxylamine hydrochloride detection method in azithromycin dry suspension, and relates to the trace detection field. The method uses isoamyl aldehyde as a derivatizing agent, generates isoamyl aldoxime by performing derivatization treatment on a sample to be measured, and then uses N-nitrosodiisopropylamine as an internal standard substance to quantitatively determine the isoamyl aldoxime by using liquid chromatography-tandem mass spectrometry. The detection method adopts an isoamyl aldehyde derivatizing agent-N-nitrosodiisopropylamine internal standard substance system, can correct detection errors caused by factors such as matrix effect, pretreatment loss and derivatization efficiency fluctuation, and improves the accuracy and sensitivity of the trace hydroxylamine hydrochloride detection result, thereby guaranteeing the safety of the medicine.

The invention discloses a method for detecting sweet-scented osmanthus aroma components in sweet-scented osmanthus wine, which comprises the following steps: diluting a wine sample, adding salt until the wine sample is saturated, and then carrying out liquid-liquid micro-extraction by using a mixed solvent of absolute ether and ethyl acetate in a specific ratio, the volume fraction of the ethyl acetate being 3-5%; after oscillation, ultrasonic-assisted extraction and centrifugal separation, an organic phase is taken for dehydration, a gas chromatograph-mass spectrometer is used for analysis, and an internal standard method is adopted for quantification. By optimizing the polarity of the solvent, various aroma components (such as leaf alcohol, linalool oxide, linalool, ionone, tea aroma spirane and the like) from strong polarity to weak polarity can be effectively covered, and balanced and efficient extraction under a single extraction step is realized. The method is easy and convenient to operate, high in recovery rate, good in reproducibility and suitable for quality evaluation and production process control of the osmanthus fragrans wine.

The invention discloses a lipid oxidative metabolism characteristic analysis method and system based on REIMS and LC-MS / MS, and belongs to the technical field of lipid oxidative metabolism detection. In order to solve the detection problems of various oxidized lipids, extremely low content in a biological sample and large metabolic fluctuation, the method provided by the invention optimizes a biological sample pretreatment process, sets accurate REIMS and LC-MS / MS technical parameters, adopts two mass spectrometry technologies for combined detection and adopts an internal standard method for quantification, so that the content of the oxidized lipids in the biological sample is greatly reduced, and the content of the oxidized lipids in the biological sample is greatly reduced. And accurate qualitation and quantification of trace lipid oxidation products in a biological sample are realized. The method has the advantages that the trace lipid oxidation products in the biological sample can be accurately qualitative and quantitative, and reliable data support is provided for mining novel biomarkers.

The application relates to an 11-dehydrothromboxane B2 detection kit and a detection method thereof, relates to the technical field of biological detection, and comprises an activation solution, an equilibrium solution, an elution solution, an elution solution, a reconstitution solution, an internal standard solution, a calibrator and a quality control product. The detection method comprises the following steps: S1, adjusting the pH of a urine sample to 2.5-3.0 by adding phosphoric acid; S2, sucking the urine sample after pH adjustment, the calibrator and the quality control product into a centrifugal tube, reacting at 37 DEG C for 2.5-3.5 hours, adding the internal standard solution, centrifuging, and taking the supernatant for solid-phase extraction treatment; S3, solid-phase extraction; and S4, calculating the concentration of 11-DH-TXB2 in the urine according to the liquid chromatogram and the peak area of 11-DH-TXB2 in the detection sample and the corresponding internal standard peak area. The application has reliable results and is convenient to calculate.

The invention discloses a method for quantitatively analyzing the content of dimethyl furandicarboxylate (DMFDCA) through a nuclear magnetic resonance hydrogen spectrum technology, which adopts an internal standard substance ethylparaben which meets the national standard and does not react with a standard substance to directly measure the content of the main component DMFDCA in the standard substance. The method comprises the following steps: dissolving ethylparaben and DMFDCA in deuterated dimethyl sulfoxide DMSO-d6 to obtain a solution to be detected, optimizing test parameters, determining a nuclear magnetic resonance hydrogen spectrum of the solution to be detected, selecting proper quantitative peaks of ethylparaben and DMFDCA, respectively integrating the quantitative peaks of ethylparaben and DMFDCA to obtain integral areas, and calculating the actual content of DMFDCA according to a formula. The method has the characteristics of simplicity, feasibility, low cost, good stability, high precision, strong specificity and the like, and has wide application prospects in the aspects of standard substance calibration, intermediate check and the like.

The invention discloses a method for determining the content of 27-hydroxycholesterol in a biological sample, and relates to the field of biochemical analysis. Comprising steps as follows: sample pretreatment: 27-hydroxycholesterol in a biological sample is extracted with an organic solvent, and a deuterated internal standard 27-hydroxycholesterole-d6 is added for correction; liquid chromatography-mass spectrometry detection is carried out, and a gradient elution procedure and a multi-reaction monitoring mode are adopted in the liquid chromatography-mass spectrometry detection, so that the 27-hydroxycholesterol is specifically separated and detected; the content of the 27-hydroxycholesterol is calculated through an internal standard method in a quantitative analysis manner, the biological sample is a serum or tissue sample, and liquid chromatography conditions comprise that an ACQUITYUPLCHSST3 chromatographic column is used, a mobile phase A is an aqueous solution containing 0.1% of formic acid, and a mobile phase B is an acetonitrile solution containing 0.1% of formic acid. The method has the advantages of high specificity, high accuracy, high sensitivity, high flux and high economical efficiency.

This invention relates to the field of analytical chemistry, and discloses an isotope dilution-bismuth reagent-LA-ICP-MS method for the determination of noble metals. The method involves adding geochemical samples to bismuth reagent kits... 194 Pt isotope diluent was mixed with a silver carrier, and then melted and blown to obtain silver alloy particles. The silver alloy particles were annealed and pressed into silver sheets. The content of Pt in the silver sheets was determined by LA-ICP-MS. 105 Pd, 195 Pt and 197 The mass spectrometry intensity of Au was determined by adding platinum isotope diluent. 194 Pt was used as an internal standard for signal correction; the contents of gold, platinum, and palladium in the sample were calculated based on the measurement results. This invention utilizes solid-state injection of prepared Ag sheets and multi-point ablation by LA-ICP-MS, improving the analysis speed and avoiding interference from polyatomic ions and dilution effects in the solution; the added platinum isotope diluent... 194 Pt, as an internal standard, is consistent with the distribution trend of the element to be measured, thus solving the problem of uneven element distribution in Ag tablets. Ag tablets prepared with standard substances serve as a standard series with consistent matrix. The standard curve is plotted using the empirical coefficient method, showing good linearity.

This invention, entitled "A Method and Application for Target Gene Quantification in qPCR Using a Quantitative Internal Standard and a Comprehensive Correction Coefficient," belongs to the field of nucleic acid detection technology. The technical problem it addresses is that existing qPCR techniques require the establishment of a standard curve using a reference sample of known concentration, increasing reagent costs and experimental complexity, and resulting in poor reproducibility. The key technical solution is to provide a method for quantitative viral load qPCR analysis of a single sample. Specifically, it provides an internal standard with a known copy number, i.e., a quantitative internal standard, and establishes a correction method. Through a systematic error compensation mechanism, it achieves accurate quantification of the target gene. This method ensures both accuracy and reproducibility while simplifying detection design in clinical applications.

The invention provides a method for simultaneously determining ritocaine and prilocaine in blood plasma based on a liquid chromatography-tandem mass spectrometry (LC-MSMS) technology. The specific operation of the method comprises the following steps: adding an internal standard solution and an organic solvent into a plasma sample, and ensuring full extraction of lidocaine and prilocaine through vortex mixing. And separating by using a centrifugal technology, and collecting supernate as a pretreated sample to be detected. An Eclipse Plus C18 chromatographic column is adopted for isocratic elution, effective separation of lidocaine and prilocaine is achieved, and then a sample is subjected to mass spectrometric detection. A standard curve is drawn based on the mass spectrum peak area ratio, a regression equation is obtained, and the concentration of lidocaine and prilocaine in the sample to be detected is obtained through calculation. The method has the characteristics of simplicity and convenience in pretreatment operation, low plasma consumption, short analysis time and high sensitivity, and is suitable for detection of lidocaine and prilocaine in plasma and clinical pharmacokinetic research of the lidocaine cream.

This invention discloses a rapid and accurate method for simultaneously determining the content of multiple new pollutants in water, belonging to the field of new pollutant detection technology. The method involves adding a compound salt and water sample to a narrow-necked, pear-shaped, flat-bottomed flask and shaking well. An internal standard solution is then added to the flask, and after shaking again, an organic extraction solvent is added. The flask is then subjected to high-frequency extraction in a vortex mixing extraction device, followed by standing. The upper organic phase in the flask is transferred, centrifuged, and the supernatant is used for ESI-HPLC-MS / MS-MRM analysis. The advantages of this invention are: by synergistically enhancing extraction through compound salting-out and mixed solvents, combined with techniques such as phenyl column chromatography and rapid gradient elution, it solves the problem of simultaneous extraction, separation, and quantification of new pollutants. Pretreatment eliminates the need for concentration, dissolution, and dehydration steps, achieving non-co-current separation of three types of substances within 10 minutes, significantly shortening the analysis time. This method is suitable for the simultaneous rapid screening and quantitative analysis of new pollutants in water bodies.

The application discloses a method for simultaneously and rapidly detecting the concentration of four immunosuppressants, comprising the following steps: mixing a blood sample with an internal standard solution containing ascomycin, cyclosporine-d4, sirolimus-d3 and everolimus-d4, adding a precipitant and centrifuging to obtain a sample to be detected; injecting into a liquid chromatography-tandem mass spectrometry system for analysis to obtain original data containing the retention time window and mass spectrum response signal of four analytes; searching the original data based on an interferent database, wherein the interferent database contains a plurality of compounds that can possibly generate mass spectrum signal interference and characteristic ion information thereof; inputting the original data and the searched potential interferent information into a machine learning model to output a net mass spectrum response value after interference correction; and calculating the concentration of tacrolimus, cyclosporine, sirolimus and everolimus in the blood sample according to the obtained net mass spectrum response value, the mass spectrum response value of the corresponding internal standard and a pre-established standard curve.

The present application belongs to the technical field of analysis and identification of fermented food, and particularly relates to a method for distinguishing and identifying fermented food of different processes based on chemical isotope labeling liquid chromatography-mass spectrometry (CIL-LC-MS) technology analysis of carboxylic acid. In order to accurately and simply identify the type and content of carboxylic acid, and thus based on carboxylic acid panorama analysis, efficiently and conveniently distinguish and identify fermented food of different processes, the present application introduces CIL-LC-MS technology to analyze carboxylic acid and thus distinguish and identify fermented food of different processes. The technology can significantly enhance the response value and detection sensitivity of carboxylic acid through chemical labeling strategy, and effectively overcome the ion suppression interference of the matrix by using isotope internal standard, so as to realize accurate quantification. Combined with its inherent three-level identification process, the present application can efficiently and conveniently distinguish and identify fermented food of different processes.

The application belongs to the technical field of thiocyanate detection, and specifically relates to a method for detecting thiocyanate based on inherent internal standard surface-enhanced Raman scattering substrate, which comprises the following steps: S1. Preparing a thiocyanate standard curve: taking the thiocyanate concentration as the horizontal coordinate, and the ratio of the peak intensity at the characteristic peak of thiocyanate with different concentrations to the peak intensity at the internal standard peak of a bare silver SERS chip as the vertical coordinate to establish a linear regression equation, thereby obtaining the thiocyanate standard curve; S2. Detecting experimental precision; S3. Quantitatively detecting the thiocyanate content: detecting the SERS signals of the thiocyanate sample to be measured and the bare silver SERS chip, and comparing the SERS signals with the thiocyanate standard curve in step S1 to obtain the thiocyanate content to be measured. In the application, the inherent peak generated by the substrate silver oxide in the bare silver SERS chip is used as an internal standard reference, no additional internal standard substance is added, the substrate detection performance is not affected, the linear fitting of the standard curve equation is better, the influence of errors is greatly reduced, the quantification is more accurate, and the thiocyanate can be rapidly detected.

The invention discloses a high-flux mixed solution quality control method based on relative response factors. According to the method, by introducing an internal standard reference substance and calculating a relative response factor Rr of each target analyte relative to the internal standard, synchronous and rapid calculation and stability dynamic evaluation of all component concentrations in a solution are realized, and the method comprises the following steps: selecting and preparing an internal standard solution; analyzing the mixed solution and the internal standard under the same condition to obtain a chromatographic peak area; calculating Rr; monitoring the change of Rr along with time to judge the component stability; and calculating the current concentration of each component based on the stable Rr value and the interior label information. According to the method, only one or a small number of internal standards are needed to replace a single standard prepared for each component in a traditional method, consumption (about 90%) and workload of standard substances are remarkably reduced, unstable components can be sensitively recognized, and an efficient, economical and reliable quality control solution is provided for high-throughput detection in the fields of environmental monitoring, food safety and the like.

The invention provides a method for detecting the content of proinflammatory regression factors (SPMs) in microalgae oil, which comprises the following steps: adding an isotope internal standard substance into a prepared microalgae oil sample to obtain an internal standard sample solution, adding an alkaline reagent into the internal standard sample solution, and carrying out saponification hydrolysis reaction to obtain an alkaline saponification hydrolysis solution; adding an acidic reagent into the alkaline saponification hydrolysis solution, carrying out acidification treatment, and adjusting the alkaline saponification hydrolysis solution to be acidic to obtain an acidified hydrolysis solution; sequentially purifying, concentrating and redissolving the acidified hydrolysis solution to obtain a solution to be detected; performing UPLC-MS / MS detection on the solution to be detected to respectively obtain a first response value of the target component and a second response value of the isotope internal standard substance; and calculating the content of the target component in the microalgae oil sample according to the first response value, the second response value and an internal standard method standard curve. Therefore, the accurate content of the SPMs in the sample can be obtained, and the detection accuracy is improved.