The invention discloses a method for producing 1,3-dioleoyl-2-palmitoyl
triglyceride. The invention belongs to the field of human milk substitutes and particularly relates to a method for producing
triglyceride, which comprises: 1, removing
cholesterol from lard by an
ethanol extraction process till the
cholesterol content reduces to below 500mg / kg; 2, reacting the lard from which
cholesterol is removed with refined
fatty acid in the presence of 1-position and 3-position selective
lipase serving as a catalyst; 3, filtering to remove
lipase from the mixed reactants; 4, removing the
fatty acid from the product, and obtaining
triglyceride; and 5, crystallizing to refine triglyceride with high OPO content. In the invention, a cholesterol removal process is introduced for the lard raw materialused in preparation, the cholesterol removal rate reaches over 92 percent, and the cholesterol content in the lard
raw material from which cholesterol is removed is lowered to less than 500mg / kg. Theaddition of the refining process improves the OPO content in the triglyceride reaches over 53 percent. The difference between the
palmitic acid content before reaction in sn-2
fatty acid and the
palmitic acid content after the reaction in the sn-2 fatty acid is less than 10 percent.