The invention discloses a KASP
molecular marker related to the
oil content of peanuts and application, and belongs to the field of molecular genetic breeding, an SNP site is positioned in a peanut A03
chromosome and named as qOCA03.1, the size of a positioning interval is in a 102kb range between A03: 8591523-8693979, the SNP site is positioned at 8641246bp of the A03
chromosome, and the polymorphism is T / C. The primer sequences of the
molecular marker are respectively as follows: a universal
forward primer F, the
nucleotide sequence of which is as shown in SEQ ID NO.1: 5 '-TTGAGCGAATACCCCATCCG-3', a universal
reverse primer F, the
nucleotide sequence of which is as shown in SEQ ID NO.2, a universal
reverse primer F, the
nucleotide sequence of which is as The nucleotide sequence of the
reverse primer R1 is as shown in SEQ ID NO.2: 5 '-CGTTGCCCTTTCGAGATAATTGTT-3'; the nucleotide sequence of the reverse primer R1 is as shown in SEQ ID NO.2; and the nucleotide sequence of the reverse primer R2 is as shown in SEQ ID NO.3: 5 '-CGTTGCCCTTTCGAGATAATTGTC-3'. The invention also discloses a kit for detecting the
disease resistance of the
human body, and a kit for detecting the
disease resistance of the
human body. According to the invention, through
whole genome association analysis of
oil content traits, significant SNP loci related to
peanut oil content are identified, and KASP primers are designed and KASP markers are developed according to
genetic variation information of positioned candidate intervals and candidate genes. Based on the KASP
typing technology, the
genotype of the peanut sample to be detected is rapidly and accurately identified, and the breeding efficiency of the peanut variety is improved.