728 results about "Aflatoxin" patented technology

The invention provides a hybridoma cell line 1C11 and an anti-aflatoxin general monoclonal antibody secreted by the same as well as the applications thereof. The hybridoma cell line 1C11 can be used for preparing a high-titer aflatoxin antibody, and a mouse hydroperitoneum antibody is measured to reach 5.12*106 by using an ELISA (Enzyme-Linked Immunosorbent Assay). The anti-aflatoxin general monoclonal antibody has high sensitivity, respectively reaches the IC50 (50% inhibiting concentration) of aflatoxin B1, B2, G1 and G2 to be 1.2, 1.3, 2.2 and 18.0 pg/mL, is the antibody with highest sensitivity among currently reported four aflatoxin antibodies, is used for measuring the total aflatoxin amounts, i.e. the total amounts of the aflatoxin B1, B2, G1 and G2 and has great practical application values.

The invention discloses a refining process for cold-pressed camellia oil at low temperature. The process disclosed by the invention comprises the following steps: processing raw materials; cold-pressing to obtain oil; carrying out high-precision filtration; hydrating and degumming; deacidifying; decolorizing; deodorizing; and dewaxing. According to the process disclosed by the invention, the harmful ingredients (for example, benzopyrene, free fatty acid, colloid, trace heavy metals, residual soap, debris, aflatoxin and the like) existing in the camellia oil and generated in the production process, and a unique unfavorable smell of the camellia oil are effectively removed; various unique natural beneficial nutrients of the camellia oil can be preserved to the maximal extent after the camellia oil is refined; and no harmful substance is generated in the processing process.

The invention discloses a method for detecting aflatoxin through an immunosensor. The aflatoxin (AFT) is a fungal toxin which has the high carcinogenicity, teratogenicity and toxicity, and accurate and rapid analysis on the aflatoxin is one of most effective means for reducing and avoiding harm of the aflatoxin. A prepared gold-polyaniline-graphene (Au-PANI-GN) composite nanomaterial is represented by adopting an ultraviolet spectrum and a transmission electron microscope and used for constructing the electrochemical immunosensor, the electrochemical immunosensor is represented by adopting a cyclic voltammetry method, an electrochemical AC impedance method and a square wave voltammetry method, and the prepared electrochemical immunosensor detects the aflatorxin B1. Experiment results show that the constructed electrochemical immunosensor has the good electrochemical activity on detection of the aflatoxin, and the concentrations of the aflatoxin and peak currents have the good linear relation, wherein the linear equation of the relation is y=-0.177x+4.28.

The invention relates to an aflatoxin B1 flow lag immunization time distinguishing fluorescence rapid-detection kit and an application thereof. The kit comprises a fluorescent test strip and a sample reaction bottle containing an europium-labeled anti-aflatoxin B1 monoclonal antibody lyophilized product, wherein the fluorescent test strip comprises a cardboard, a water absorption pad, a detection pad and a sample pad are sequentially pasted on one surface of the cardboard from top to bottom, adjacent pads are connected at the connection in an overlapping manner, the detection pad treats a cellulose nitrate membrane as a base pad, a transverse quality control line and a detection line are arranged on the cellulose nitrate membrane from top to bottom, the quality control line is coated with a rabbit anti-mouse polyclonal antibody, and the detection line is coated with an aflatoxin B1 bovine serum albumin conjugate; and the anti-aflatoxin B1 monoclonal antibody is secreted by a hybridoma cell strain having a preservation number of CCTCC NO.C201015. The kit can be used for the quantitative determination of the content of the aflatoxin B1, and has the advantages of simple operation, rapidness and high accuracy.

The invention relates to a method for reducing the heavy metal content of rice and products thereof. The method comprises the steps of soaking in alkaline liquid, separation, reaction, washing, drying and the like; the process realizes different degrees of removal effects on aflatoxin and heavy metals such as cadmium, arsenic, lead and mercury, and can effectively reduce the heavy metal content of rice and rice products such as rice noodles, starch, rice protein powder and the like prepared from rice; the method provided by the invention is simple in product, low in production cost and easy to implement; the heavy metal index of the produced product conforms to the requirements of national standard.

The invention discloses a novel method for rapid detection of aflatoxin in food. The novel method studies extraction, purification and enrichment of aspertoxin in food and detects aflatoxin in food by using a combination of high performance liquid chromatography, on-line post-column photochemical derivatization and a fluorescence detector. The method employs a combination of dispersive magnetic solid-phase micro-extraction and dispersive liquid-liquid micro-extraction techniques for extraction, purification and enrichment of aspertoxin in a sample. Compared with a conventional determination method for aflatoxin, the method provided by the invention has the characteristics of a small amount of an organic solvent used in detection, short analysis time, simple operation, low detection cost, accuracy, etc.

The invention provides an online aflatoxin detecting device. The online aflatoxin detecting device comprises multiple light source transmitting and signal receiving integration units distributed at the two sides of a material track, and an upper computer. Each illuminating system comprises an ultraviolet source and a rotating reflection mirror which reflects the ultraviolet source to generate a line light source in the material perpendicular direction. Each signal receiving integration unit receives signals of multiple fluorescence wavelengths of bounce light at the same position of the surface of the same material, and converts the signals into electrical signals. The upper computer selects the signals of any three paths to be synthesized into a color image. A sensor in each optical signal receiving device is a single-point photoelectric detector. The invention further discloses material sorting equipment adopting the detecting device. The detecting device has the advantages that the weak fluorescence signals on the surfaces of the materials are effectively detected, each detected material point is only detected by the corresponding single-point photoelectric detector once, signal superposition is avoided, influence of material rolling, jumping and the like is eliminated, and the materials contaminated by aflatoxin and the normal materials are effectively distinguished.

The invention discloses a test strip for detecting aflatoxin B1 or M1 by utilizing an aptamer. The test strip for detecting the aflatoxin B1 or M1 by utilizing the aptamer, provided by the invention, comprises a sample absorption pad, a marker pad, a reaction film and a water absorption pad, wherein the marker pad is coated with a detection probe; the detection probe is an aflatoxin B1 aptamer marked by fluorescein; the nucleotide sequence of the aflatoxin B1 aptamer is sequence 1; the reaction film comprises a detection region and a quality control region; the detection region is coated with a conjugate formed by an aflatoxin B1 hapten and a carrier protein; and the quality control region is coated with a quality control probe, and the quality control probe is a conjugate formed by avidin conjugation probe marked aflatoxin B1 complementary single strand DNA (Deoxyribose Nucleic Acid)) molecules. The test strip for detecting the aflatoxin B1 or M1, provided by the invention, has the advantages of high sensitivity, accuracy in quantifying, strong specificity, simplicity and convenience, and short detection time.

A nondestructive system and method for aflatoxin detection based on red-orange fluorescence is described. A sorting plane is illuminated with a wide band black light source. At least one image of unsorted produce on said sorting plane is obtained. A red component of the at least one image is evaluated. Contaminated produce is determined based on the red component.

The invention discloses a non-transgenic soybean oil cold pressing preparation process which is characterized by including the steps of raw material selection and polishing and dehulling, pressing moisture adjustment, pressing and residual oil control, crude oil filtering, removal of lipoxygenase by hyperfiltration and removal of beany flavor by bumping in vacuum at the low temperature. Non-transgenic soybeans serving as raw materials eliminates potential hazard possibly caused by transgenic raw materials, and using the physical cold pressing technology enables a product to be free from organic solvent residues, eliminates contamination of aflatoxin on the raw materials and biological factors resulting in deterioration during soybean oil storage, effectively removes the beany flavor and other peculiar smell in soybean oil and is rich in natural antioxidants such as VE (vitamin E) and the like so that extra antioxidants do not need to be added during oil storage.

The invention belongs to the technical field of biology, and relates to an immunoaffinity adsorption material based on a single-domain heavy-chain antibody, in particular to an immunoaffinity adsorption material for aflatoxin. The material comprises a carrier and a ligand carried by the carrier, and is characterized in that the single-domain heavy-chain antibody specially recognizing aflatoxin is used as the ligand and has the amino acid sequence shown in SEQ ID NO.:1and the like. The antibody has the advantages of being resistant to acid, alkali and high temperature, easy to produce and the like, and important practical value is achieved for a low-cost immunological detection method which is used for aflatoxin and can be repeatedly used.

The invention provides a method for detecting SERS of aflatoxin B1 molecules based on molecularly imprinted polymer coated gold core-shell nanoparticles, and relates to surface reinforced raman detection. According to the method, core-shell nano-particles taking gold nanoparticles as a core and molecularly imprinted polymer as a shell are synthesized by coating aflatoxin B1 molecularly imprinted polymer around the gold nanoparticles; the hole in the molecularly imprinted polymer can be used for specifically capturing the aflatoxin B1 molecules after template molecules in the molecularly imprinted polymer of the shell layer are eluted, and surface reinforced raman detection of aflatoxin B1 molecules can be realized since the aflatoxin B1 molecules are in an effective range of a gold nanoparticle plasma resonant magnetic field; and the molecularly imprinted polymer is obtained by eluting the molecularly imprinted shell layer prepared by using the aflatoxin B1 molecules as template molecules, and has highly specific adsorption for aflatoxin B1, and can be used as a SERS substrate for quantitative detection of aflatoxin B1. The method has the characteristics of high specificity and short sample analyzing time.

The invention belongs to the technical field of activated carbon, and particularly relates to a peanut shell activated carbon for removing toxins in peanut oil and a preparation method thereof. The peanut shell activated carbon is prepared by carrying out pretreatment, extrusion molding, carbonization and activation on peanut shells. The preparation method comprises the following steps: pretreatment: carrying out hydrothermal treatment on the peanut shells, and pulverizing; extrusion molding: carrying out pressure molding with a granulator to obtain cylindrical granules of which the diameters are 4-8 mm and the lengths are 10-20 mm; heating, cooling in the absence of oxygen, crushing and screening to control the granule diameters at 1-3 mm; and introducing isothermal superheated vapor, storing in a sealed environment, and cooling to obtain the finished product. The activated carbon is prepared from the peanut shells which are accessible raw materials, thereby implementing utilization of waste peanut shells; and the obtained peanut shell activated carbon has the advantages of large specific area and small pore size, can simultaneously adsorb benzopyrene, aflatoxin and other harmful substances, and can prevent the peanut oil from oxidative rancidity.

The invention relates to a natural cleaning agent for removing residual pesticides on fruit and vegetable surfaces and a preparation method; the preparation method of the cleaning agent comprises the following steps: cleaning shells, removing heavy metal, sterilizing the shells, performing high temperature electrolysis and ionization of the sterilized shells to form solid powder, and refining with a mesh sieve to prepare nanometer powder; the cleaning agent comprises one or more than one natural shells according to certain weight ratios. The cleaning agent of the invention not only can remove residual toxic materials on fruit and vegetable surfaces, such as pesticides, sulfur dioxide and aflatoxin, prevent toxic materials from entering human body by eating of the fruit and vegetable, thus prevent continuous accumulation of the toxic materials in the body to damage human body health, but also has no toxicity or irritation to human skin, causes no secondary pollution of ecological environment, meets the development trend of green cleaning agents, and has good application prospects.

The invention discloses a production method of edible rice bran oil. The production method comprises the following steps of: firstly, heating four-stage standard rice bran oil by a preheater and charging into a separator, evenly distributing the materials under high vacuum degree, due to the boiling point difference of various substances, separating light components of raw material oil in an evaporating way by a pressure reducing technology, heating heavy components, charging into a purifier, quickly evaporating, separating aflatoxin and pesticide residue in an evaporating way, filtering the treated rice bran oil, contacting with protective gas in a stripping tower, and discharging in a separating way, wherein the acid value of the rice bran oil discharged out of the tower bottom achieves the standard of first-stage rice bran oil; and dewaxing in a winterization way to obtain the first-stage rice bran oil. According to the production method, the first-stage rice bran oil product with high oryzanol content can be produced. The whole technical process separates by a pure physical method, so that nutrition components can not be damaged; the production method does not need adding of any annexing agents, so that the safety of the product can be guaranteed; and the production method does not generate any waste gas, sewage or solid waste.

The invention relates to a dual-label rapid response nucleic acid adapter probe, which consists of a nucleic acid adaptor, the 5' end and 3' end of which are labeled by a fluorescent chromogenic grouprespectively, and a complementary probe, the 5' end and 3' end of which are labeled by a fluorescent quenching group respectively. The nucleic acid adaptor is a nucleic acid adaptor which can especially identify aflatoxin B1, namely, AFB1 nucleic acid adaptor. The nucleotide sequence of the AFB1 nucleic acid adaptor is shown as SEQ ID NO: 1 in the sequence list. The complementary probe is complementary to two ends of the AFB1 nucleic acid adaptor. The nucleotide sequence of the complementary probe is shown as SEQ ID NO: 2 in the sequence list. The method can be used for rapidly detecting aflatoxin B1. The identification process, after the aflatoxin B1 is added, can be completed in one minute, and one aflatoxin B1 molecule can trigger the recovery of two fluorescent group signals with highsensitivity. The whole reaction is carried out in a homogeneous and constant temperature solution environment, and the operation is simple.

The invention belongs to the field of molecularly imprinted polymers, and relates to a quercetin surface imprinted polymer and application of the quercetin surface imprinted polymer in detection of toxins of wheat and other crops. Quercetin is used as an alternative template of the polymer, UAO-66-NH2 is selected as a carrier material for surface imprinting, and the surface imprinted polymer is prepared by using a chemical grafting method. The synthesized polymer is used as an adsorbent to prepare a surface imprinted solid phase extraction column, and aflatoxin in food is extracted and separated, so that a simple, rapid, efficient and sensitive method for detecting aflatoxin in wheat samples is formed. When the separation enrichment effect is compared with that of commercial columns and immunoaffinity columns, aflatoxin can be adsorbed selectively, and the selectivity and adsorption capacity of aflatoxin are better; and furthermore, development of sample pretreatment for crop toxin detection is facilitated, and the problems of high cost and large time consumption of current pretreatment methods are solved.