333results about How to "Superparamagnetic" patented technology

The invention discloses a magnetic gold nanoparticle composite material, and a preparation method and application thereof. The preparation method comprises the following steps: synthesizing ferroferric oxide magnetic microspheres, adding tetraethoxyl silicon to implement hydrolysis and polymerization on the magnetic microsphere surfaces, adding N-(2-aminoethyl)-3-aminopropyltrimethoxysilane to obtain surface-modified double-amino-group Fe3O4 / SiO2 magnetic microspheres, and compounding the surface-modified double-amino-group magnetic microspheres with gold nanoparticles. The chelation action of the double amino groups is utilized to adsorb the gold nanoparticles more firmly; and the material combines the surface selectivity of the gold nanoparticles and the superparamagnetism of the magnetic nanoparticles, can extract benzopyrene in water, and can implement quick and efficient separation.

The invention discloses a magnetic carbon nanotube composite material and a preparation method and an application thereof. The preparation method comprises the following steps: adding FeCl3.6H2O to an ethylene glycol solution; then adding anhydrous sodium acetate and polyethylene glycol; adding a carboxylated multiwalled carbon nanotube; performing ultrasonic dispersion; adding hexamethylenediamine or ethanediamine; heating to 200-300 DEG C for reaction for 8-24h; and washing and performing vacuum drying to prepare the amino-modified magnetic Fe3O4-carbon nanotube composite material. The amino-modified magnetic Fe3O4-carbon nanotube composite material prepared by the invention is of nanoscale and superparamagnetism, can be stably dispersed in solutions, and can be quickly separated and enriched through a simple action of a magnetic field. As an adsorbent, the material is large in superficial area and has various active groups on the surface. The material can adsorb pigments from complex substrates through pi-pi electron interaction and hydrophobic effect of the carbon nanotube and can adsorb compounds such as organic acids and phenols through weak anion exchange effect of amino groups on the surfaces of magnetic nanoparticles.

The invention discloses a magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 and a preparation method thereof. The method for preparing the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 comprises the steps of: firstly preparing hydrophobic monodisperse Fe3O4 nano-particles by adopting a chemical oil-phase high-temperature method, and modifying the surfaces of the hydrophobic Fe3O4 nano-particles to ensure that the hydrophobic Fe3O4 nano-particles are dispersed in a water phase; preparing luminescent CdTe quantum dots of which the surfaces are provided with carboxyl groups, and precipitating the luminescent CdTe quantum dots on the surfaces of the magnetic Fe3O4 nano-particles through the co-precipitation; then utilizing ligand exchange to modify a silane coupling agent on the surfaces of the luminescent CdTe quantum dots; and finally forming an outermost SiO2 coating layer through silane or silicon ester hydrolysis. The diameter of the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 is between 30 and 50nm; the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2has double functions of magnetism and fluorescence at the same time, has strong and durable fluorescence intensity after labeling rat bone marrow-derived mesenchymal stem cells, and apparently reduces cellular magnetic resonance signals. The particle has broad application prospect in the fields such as biological labeling, bioseparation and the like.

The invention discloses a preparation method of magnetic / gold nano particles. The preparation method comprises the following steps: firstly adopting a co-precipitation method to prepare the magnetic Fe3O4 nano particles; polymerizing the dopamine in-situ on the surfaces of the magnetic particles to obtain Fe3O4 nano particles modified by the poly dopamine; introducing polyphenol and amino groups to the surfaces of the Fe3O4 nano particles; absorbing the nano gold seeds on the surfaces of the modified magnetic particles through the static action; adopting the nano gold which is absorbed on the surfaces of the magnetic particles as the seed, adopting the polyphenol on the surfaces of the magnetic particles as a reducing agent, gradually adding the chloroauric acid liquid to gradually produce the gold layers on the surfaces of the magnetic particles to obtain the core-shell magnetic / gold nano particles. The nano particles have good water dispersion and strong magnetic respond performance. The diameters of the nano particles are 30-100 nanometers, the saturation magnetization is 30.1-38.7emu / g, and the nano particles are superparamagnetic. The nano particles have wide application prospect on the fields of targeted drug controlled release, thermal therapy, separation of protein and enzyme, etc.

The invention relates to a preparation method of a protein magnetic imprinting nano sphere, in particular to a preparation method of a protein magnetic imprinting nano sphere which takes one of hemoglobin, muramidase or serum protein as template molecules; the preparation method includes the following steps: synthesizing magnetic nano particles which are Fe3O4 nano particles covered with silicon dioxide on the surfaces; carrying out silanization to the amino-groups on the surface of the magnetic sphere; using glutaric dialdehyde to connect protein; using silylation agent to fix the space structure of template protein; alkaline eluting the protein on the surface of the magnetic sphere to form imprinting sites. The hemoglobin, muramidase or serum protein magnetic nano imprinting polymer sphere prepared by the invention combines the precise and specific identification property of molecular imprinting and the quick separation property of the magnetic nano sphere under the action of an external magnetic field, integrates the advantages of molecular imprinting and the magnetic nano sphere, avoids complex centrifugation operation, plays vital roles in the separation of cells, protein and nucleic acid, the detection of biomolecules, tumor diagnosis and medicine targeting therapy and has promising application prospect in the field of isolation analysis.

The invention discloses a preparation method of a magnetic carbon nanotube with polydopamine-modified surface, belonging to the field of nanomaterial preparation. The method comprises the following steps: synthesizing magnetic nanoparticles by adopting a solvothermal method; adding the magnetic nanoparticles and a carbon nanotube into ethanediol according to a certain ratio, stirring and ultrasonically processing to obtain a uniform solution, and performing solvothermal reaction on the solution to obtain the magnetic carbon nanotube; dissolving the obtained magnetic carbon nanotube and dopamine in Tris-HCl buffer liquid according to a certain ratio, and polymerizing for a certain time to obtain the magnetic carbon nanotube with the polydopamine-modified surface. The magnetic carbon nanotube prepared by adopting the method has ultrastrong mechanical property of the carbon nanotube, the characteristics of nanometer size effect, relatively good biocompatibility, magnetic responsibility and the like of the magnetic particles and the characteristics of hydrophilicity, structural stability and a secondary reaction platform of dopamine, and can be widely applied in the fields of biology such as protein separation, drug delivery and biological marking.

The present invention provides a preparation of a chitosan compound adsorbent for absorbing magnetic powder heavy metal ion imprint. The chitosan is employed as a imprinting matrix material, the nanometer magnetic powder Fe3O4 is employed as a magnetic component, the epichlorohydrin is employed as a crosslinking agent, the sodium tripolyphosphate is employed as a curing agent, after steps of ion imprinting, magnetic powder embedding and curing and template ion eliminating, the magnetic metal ion imprinting chitosan compound adsorbent is obtained. The compound absorbent prepared by the present invention can be separated from the solution after absorption efficiently and rapidly under the external magnetic field to realize the rapid efficient reclaim of the adsorbent. The compound absorbent has high mechanical strength and selective absorbability of imprint Cr(VI) or Zn (II), avoids the swelling loss of the chitosan in acidity solution and improves the absorption capacity of Cr(VI) or Zn (II). The preparation of the present invention has simple reaction conditions and has no rigorous limit of the experiment conditions. The preparation of the present invention can be used to process the heavy metal waste water containing Cr(VI) or Zn (II), also reclaim Cr(VI) or Zn (II).

The invention belongs to the field of preparation of water treatment materials, and in particular relates to a preparation method of magnetized silicon dioxide aero-gel. The preparation method comprises the following steps of: with water glass as a silica source, adding nano-grade ferroferric oxide dispersed liquid and a drying control chemical additive to obtain the magnetized silicon dioxide aerogel at ordinary pressure and drying conditions through a sol-gel method and surface modification. The magnetized silicon dioxide aerogel is a nano-grade porous material, the average pore size of each pore is 3-5nm; the magnetized silicon dioxide aerogel has a saturated magnetization intensity of 0.4-0.8emu.g-1. The preparation method has low cost; and the magnetized silicon dioxide aerogel prepared by adopting the preparation method disclosed by the invention has magnetism, can realize the solid-liquid separation effectively under the action of an external magnetic field, and is suitable for mass production.

The invention relates to a preparing method ofa nano-magnetic polymer composite microsphere, which comprises the following steps: preparing a hydrophilic Fe3O4 magnetic liquid particle by using a chemical co-precipitation method; dissolving initiating agents and emulsifying agents by weight part into ionized water to prepare water phase, adding the hydrophilic Fe3O4 magnetic liquid particle and adding a monomer to obtain nano-magnetic seed emulsion through polymerization; dissolving initiating agents by weight part into ionized water, adding the nano-magnetic seed emulsion and then adding a monomer to obtain nano-magnetic polymer composite microsphere emulsion through polymerization; the emulsifying agents are sodium lauryl sulfate, sodium dodecyl sulfate or sodium dodecyl benzene sulfonate; the initiating agents are potassium peroxydisulfate, ammonium persulphate or sodium peroxydisulfate; the initiating agents are potassium peroxydisulfate, ammonium persulphate or sodium peroxydisulfate; the hydrophobic monomer is styrene or methyl methacrylate or a mixture of the styrene and the methyl methacrylate; the nano-magnetic polymer composite microspheres prepared by adopting a seed emulsion polymerization method have the superparamagnetism, have stronger magnetic responsiveness, have no uncoated magnetic particle and contain magnetic polymer microspheres and are narrow to distribute.

The invention relates to a magnetic thermometer nm particle with biocompatibility, which has biocompatibility, and double response properties of magnetism and thermometer property, and the particle diameter thereof is 20 to 60 nm, and the magnetic responsibility is strong, and magnetic saturation intensity is 10.0 to 27.0 emu / g. The synthetic method of the magnetic thermometer nm particle with biocompatibility comprises: firstly, adopting chemical coprecipitation method to prepare magnetic Fe3O4 nm particle, and using silane coupler to hold surface modification, and then using the modified magnetic Fe3O4 nm particle as a seed, and having polyreaction with dextran, N-isopropyl acrylamide, finally obtaining the magnetic thermometer nm particle with biocompatibility. The magnetic thermometer nm particle with biocompatibility synchronously has double responses of magnetism and thermometer property, and has wide application prospect in the fields of drug release, protein and enzyme separation.

The invention relates to a method for preparing a TiO2 / PS / Fe3O4 magnetic nanoparticle photocatalyst, comprising the following steps of: (1) preparing oleic acid modified Fe3O4 nanoparticles; (2) preparing an aqueous-phase magnetic fluid; (3) preparing styrene miniemulsion; (4) preparing magnetic polystyrene beads PS / Fe3O; and (5) preparing the magnetic photocatalyst TiO2 / PS / Fe3O4, which specifically comprises the following steps of: mixing absolute ethyl alcohol with tetra-n-butyl titanate, and performing magnetic stirring to form a solution A; adding the magnetic polystyrene beads PS / Fe3O to de-ionized water and performing ultrasonic treatment to form a solution B; under magnetic stirring, adding the solution A to the solution B, thereby obtaining sol after 30-40 min, wherein TiO2 covers the PS / Fe3O at the moment; after condensing and refluxing the sol in a water bath, filtering the sol to obtain the TiO2 / PS / Fe3O4, washing the TiO2 / PS / Fe3O4 by using ethanol, filtering, washing by using distilled water and filtering, thus obtaining a solid; and drying the solid until the weight thereof is constant, thereby obtaining the magnetic photocatalyst TiO2 / PS / Fe3O4 with the polystyrene PS as an isolating layer, the Fe3O4 as a magnetic core and the TiO2 as a shell. The product obtained by using the method is low in energy consumption, high in catalytic activity and recyclable.

The invention discloses a surface-aminated Fe3O4 nano particle and a preparation method and the application thereof. The method comprises the following steps: (1) in inert atmosphere, FeCl2 and FeCl3 are added into the mixed solution of sodium hydroxide aqueous solution and hydrochloric acid aqueous solution for reaction and Fe3O4 nano particles are obtained; (2) ethanol solution of the Fe3O4 nano particles is prepared; in inert atmosphere, strong ammonia water and tetraethoxysilane are added into the ethanol solution of the Fe3O4 nano particles and after reaction, modified Fe3O4 nano particles are obtained; and (3) the ethanol solution of the Fe3O4 nano particles with a good dispersion effect is prepared; in inert atmosphere, 3-aminopropyl triethoxy silane is added into the ethanol solution of the modified Fe3O4 nano particles and after reaction, the surface-aminated Fe3O4 nano particles are obtained. The aminated Fe3O4 nano particles provided by the invention can have good adsorption capacity to 5 types of phenylarsonic acid at the same time. At the same time, as the aminated Fe3O4 nano particles have superparamagnetism, centrifugalization is unnecessary for sample treatment. Thus, the steps of pretreatment of samples are greatly simplified, the pretreatment time is shortened and the efficiency of the pretreatment of samples is increased.

The invention relates to a preparation and application of a new macromolecule lipidosome. The structure is a vesicle which has a lipide dual-layer structure containing duoparental chitosan longchain alkyl quaternary ammonium salt as well as phospholipid with micromolecule. The chitosan longchain alkyl quaternary ammonium salt is characterized in that: the weight average molar mass is bigger than 2000; the chitosan longchain alkyl quaternary ammonium salt can be dissolved in chloroform and other organic solvent, which contains carboxyl, amino or quaternary ammonium salt radical and concretely is one of the duoparental chitosan longchain alkyl quaternary ammonium salt disclosed by 200710056993.4 of China Invention Patent. The surface of the macromolecule lipidosome can be connected with various Liver-Targeted preparations. The macromolecule lipidosome can carry water-soluble, oil-dissolving or duoparental materials including medicine, protein, gene, nutrition, vitamin, magnetic granule and quantum dot, etc. The macromolecule lipidosome has the advantages of high wrapping rate, simple and convenient operation, strong applicability and low cost; the macromolecule lipidosome after being covered has even distributed granule diameter which can be lower than 20nm for the smallest, has stable system and strong functions for carrying medical tiny balls and slowly controlling as well as releasing.

The invention discloses a synthetic method and application of a sliver / ferroferric oxide / silicon dioxide / titanium dioxide four-layer nuclear shell structure. The synthetic method of the sliver / ferroferric oxide / silicon dioxide / titanium dioxide four-layer nuclear shell structure comprises the steps of firstly preparing a sliver / ferroferric oxide nuclear shell structure by using a solvothermal method; then preparing a sliver / ferroferric oxide / silicon dioxide three-layer structure on the basis of the nuclear shell structure by using a stober method; coating a layer of burr shape titanium oxide composed of nanosheets on the surface of the three-layer structure obtained in the step B by using the solvothermal method to obtain a sliver / ferroferric oxide / silicon dioxide / titanium dioxide (AgFe3O4SiO2TiO2) four-layer nuclear shell structure. The four-layer nuclear shell structure is good in absorption characteristic, photocatalytic performance and superparamagnetism for removal of organic pollutants or heavy metal pollutants, so that the treatment effect of the organic pollutants and the heavy metal pollutants in sewage can be greatly improved.

The invention relates to a magnetic bionic adsorbent and an application of the magnetic bionic adsorbent in treating acid wastewater containing uranium. A solvothermal method for preparing Fe3O4 by taking PSSMA (poly (4-styrene sulfonate-copolymer-maleic acid) sodium salt) as a surfactant is improved, grain sizes are uniform, and Fe3O4 magnetic nanoparticles can be synthesized on a large scale; biomolecule dopamine is polymerized in a trihydroxymethyl aminomethane buffer system and coats the surfaces of the Fe3O4 magnetic nanoparticles, and the novel magnetic bionic adsorbent with a core-shell structure is established. The magnetic bionic adsorbent has excellent hydrophilicity and larger specific surface area due to the the polydopamine structure of a shell layer, rich functional groups on the surface of the magnetic bionic adsorbent have high uranium adsorption capacity in a wider acidity range, the magnetic bionic adsorbent has higher uranium adsorption capacity and good selectivity, and uranium adsorbed on the surface of the adsorbent can be easily washed with a 0.1 mol / L (NH4)2CO3 solution and can be recycled conveniently.

The invention discloses an adsorbing agent based on ferroferric oxide and graphene and a preparation method of the adsorbing agent. The adsorbing agent based on the ferroferric oxide and the graphene is composed of oxidized graphene and ferroferric oxide, wherein the mass ratio of oxidized graphene to ferroferric oxide is 1:1-8:1. The process is simple, raw materials are low in price, and the adsorbing agent is green and non-toxic, can be produced in batches, has high adsorption performance and superparamagnetism, is capable of efficiently adsorbing pollutants such as heavy metal ions and is capable of performing quick separation by virtue of the control of an external magnetic field.

The invention relates to a magnetic flocculant and a preparation method thereof, wherein the magnetic flocculant is cationic polyacrylamide-modified magnetic nanometer Fe3O4 particles. The magnetic flocculant provided by the invention retains the advantages of low dosage of organic high molecular flocculant, high flocculation speed, coexistence of salts, low pH value and temperature influence, low total amount of flocculating constituent and the like; and the superparamagnetism of the flocculant enables the flocculant to quicken the precipitation under the effect of an applied magnetic field and to realize quick and efficient separation of residual flocculant and flocculation. The magnetic flocculant provided by the invention has the advantages of simple preparation technique, low cost, easiness in amplification and no secondary pollution, and facilitates realizing efficient and economic flocculation operation.

The invention relates to a method for preparing ternary compound anatase titania photocatalyst. Wherein, it uses magnetic nanometer particle sol and active carbon whose mass ratio is 1:1-1:9; then puts the active carbon into magnetic nanometer particle sol, to be mixed; therefore, the magnetic nanometer particles are adsorbed by the active carbon; then separating them via magnetic field to be dried at 40-80Deg. C, to obtain magnetic active carbon whose carrier is titania; then emerging the magnetic active carbon in anatase titania sol, to make the mass ratio between the magnetic active carbon and anatase titania 1:1-1:5; mixing for 0.5 hour and evaporating the suspension into powder; said powder is the final compound photocatalyst. The invention can prepare porous active carbon with high adsorption ability.

The invention discloses a surface modified nano ferroferric oxide Fenton catalyst and a preparation method thereof. The preparation method of the surface modified nano ferroferric oxide Fenton catalyst comprises the following steps: firstly preparing high dispersibility nano ferroferric oxide by adopting a coprecipitation method, and then carrying out surface modification on the nano ferroferric oxide by adopting a hydrolysis method. Organic acid salt and a non-ionic surface active agent are added when the coprecipitation method is adopted for preparing ferroferric oxide particles, so that the quantity of inorganic matters deposited on the surfaces of the ferroferric oxide particles is low, enrichment of reaction substrate and charge transfer on the surfaces of the ferroferric oxide particles can be enhanced, good catalytic efficiency can be realized at room temperature in a larger pH range, and the surface modified nano ferroferric oxide Fenton catalyst can be used for advanced oxidation treatment of dye sewage, organic pollutant sewage, pesticide sewage and the like. Therefore, the preparation method of the surface modified nano ferroferric oxide Fenton catalyst has the characteristics of simplicity in operation, mild conditions, adaptability to large-scale preparation and the like, cost for obtaining the product is low, and the obtained product can be widely and more easily popularized.

The invention discloses a hepatitis B surface antibody (HBsAb) magnetic particle chemiluminescent immunoassay kit and a preparation method thereof and the kit can be used to test the content of HBsAb in human serum fast and accurately. The kit of the invention comprises a mixed liquor of hepatitis B surface antigen (HBsAg) coated with magnetic particle and bioti-labeled HBsAg, horse radish peroxidase-labeled streptavidin, HBsAb calibrator, chemiluminescence substrate, concentrated cleaning solution and reaction tubes. According to the principle of the double antigen sandwich method, the magnetic particle immunity technology, biotin-avidin technology and chemiluminescence immunoassay technology are combined in the kit of the invention which has the advantages that the testing is fast, the linear range of the curve is wide, and the calibrator calibrated by national linear reference can be used to test the content of HBsAb in human serum accurately, thus processing important clinical application value.

A monometer-level microsphere of magnetic polymer is prepared from the hydrophilic monomers (acrylamide, acrylic acid, etc.) through preparing super-paramagnetic nm microspheres of iron oxide by chemical deposition method, adding emulsifier, trigger, cross-linking agent and said iron oxide microspheres, and reaction in redox or thermal triggering system. The resultant microsphere has super paramagnetism and adjustable granularity (70-140 nm).

The invention relates to a tumor-targeted T1-T2 double nuclear magnetic resonance imaging contrast agent and a preparation method and an application thereof. The contrast agent comprises hyaluronic acid-coated ferroferric oxide composite magnetic nanoparticles, wherein the molecular formula of hyaluronic acid is as shown in the specification; n is an integer of 17-290. The preparation method of the contrast agent comprises the following steps: (1) dissolving hyaluronic acid into deionized water, introducing a gas, and heating to obtain a reaction system A; (2) dissolving ferric salt and ferrous salt into a strong acid to obtain a solution B; (3) injecting the solution B into the reaction system A, adjusting the pH to be alkaline, and then refluxing at a high temperature to obtain a reaction system C; and (4) cooling a reaction system C to a room temperature, and dialyzing to obtain the contrast agent. The invention further provides an application of the contrast agent in T1 and T2 weighted imaging in in-vivo and in-vitro nuclear magnetic resonance. The contrast agent provided by the invention has superparamagnetism and outstanding T1 and T2 relaxation enhancement effects, and is suitable for being used as a T1-T2 double nuclear magnetic resonance imaging contrast agent.

The invention relates to a preparation method for a superparamagnetism ferroferric oxide sub-micron hollow microsphere. The preparation method comprises the following steps: 1) taking a reducing agent, an iron source and an alkali source, and dissolving into distilled water; 2) dissolving a surfactant into a solution obtained from step 1); 3) introducing a solution obtained from step 2) into a reaction kettle, reacting in a drying oven, performing natural cooling to room temperature for seasoning, water washing, alcohol washing, and drying in a vacuum drying box, so that the superparamagnetism ferroferric oxide sub-micron hollow microsphere is obtained. The preparation method has the following good effects: 1) according to the invention, the ferroferric oxide sub-micron hollow microsphere is compounded, and has superparamagnetism, high saturation magnetization and excellent oxidation resistance; 2) dispersing agents such as PEG (polyethylene glycol) and PVP (polyvinylpyrrolidone) are adopted to solve the problem of magnetic microsphere agglomeration, so that the ferroferric oxide sub-micron hollow microsphere with uniform particle size is obtained; 3) according to the invention, adopted raw materials is cheap, so that cost is minimized.

The invention relates to a preparation method of superparamagnetic composite microballoons used in biomedicine. First, a coprecipitation method is used to prepare superparamagnetic Fe3O4 nanoparticles, and after modified by a surfactant, the prepared Fe3O4 nanoparticles are dispersed into deionized water to form a water-based magnetic liquid. Second, inorganic / organic core shell microballoons with carboxyl function groups on surfaces are prepared. The superparamagnetic composite microballoons are of an inorganic / organic core shell structure, have composite material characteristics and biological effects. The microballoons have active function groups on the microballoon surfaces, and therefore can be combined with a plurality of biologically-active substances. The magnetic microballoons prepared by the method of the invention have active carboxylic groups on the microballoon surfaces, and have superparamagnetism and a large magnetic content, and therefore the microballoons are subject to magnetization separation under an externally applied magnetic field and can lose magnetism immediately after the magnetic filed is removed. The magnetic microballoons prepared in the invention can be widely applied in the detection and separation operation of tumor cells in the field of biomedicine. The method has simple experimentation, is rapid, and has low cost.

The invention discloses a magnetic particle chemiluminescence immunoassay kit of free thyroxine (FT4), belonging to the medical field of immunoassay. The kit comprises: 1) free thyroxine series calibrator; 2) magnetic particle solution enveloped by anti-fluorescein isothiocyanate monoclonal antibody; 3) free thyroxine antibody solution marked by fluorescein isothiocyanate; 4) free thyroxine markedby horse radish peroxidase; 5) chemiluminescence substrate liquid; and 6) concentrated washing liquid. The invention also discloses a preparation method of the kit. The kit in the invention has advantages of simpleness, convenience, rapidness, sensitivity, stability and the like.

The invention relates to magnetic graphene oxide composite material immobilized horse radish peroxidase as well as a preparation method and application thereof, belonging to the technical field of inorganic materials and analysis. The preparation method comprises the steps of firstly preparing a magnetic polymer microsphere containing 6-arm polyethylene glycol amide (6-arm-PEG-NH2) from magnetic graphene oxide GO-Fe3O4 and 6-arm-PEG-NH2, immobilizing horse radish peroxidase, and degrading pollutant phenol by virtue of immobilized horse radish peroxidase. The synthetic process of the material is reasonable in design, and the carrier material is coupled with zymoid catalytic activity of graphene oxide and high density amino functional groups on the surface of a multi-arm polymer 6-arm-PEG-NH2, so that the activity and reutilization capacity of immobilized horse radish peroxidase are effectively improved; furthermore, the degradation velocity of prepared immobilized horse radish peroxidase is obviously higher than the degradation velocities of free enzyme and a carrier material.

The invention provides a metatitanic acid lithium ion sieve and a preparation method of the lithium ion sieve. A molecular formula of the lithium ion sieve is H2RxTi1xO3; R is a magnetic element; x isbetween 0.01 and 0.2; and the lithium ion sieve is formed by doping the magnetic element into an Li2TiO3 lattice, substituting part of titanium positions and removing lithium by pickling. A lithium ion sieve precursor is prepared by calcining a lithium source, a titanium source and a superparamagnetic substance and subjected to pickling and liquid-solid separation to form the magnetic separable metatitanic acid lithium ion sieve. The prepared metatitanic acid lithium ion sieve has the advantages of high adsorption capacity and leaching rate, low solution loss rate, stable structure and the like; the adsorption capacity of the lithium ion sieve is above 50mg / g; the leaching rate is greater than 99%; the solution loss rate is less than 0.1%; and the lithium ion sieve can be repeatedly usedfor many times. After adsorption, the lithium ion sieve can be separated from a lithium containing solution quickly by the action of an externally applied magnetic field, and the separation time is 1-10min.

The invention discloses a functionalized Fe3O4 nano-particle and a preparation method and application of the functionalized Fe3O4 nano-particle. The preparation method comprises the following steps of: (1) under inert atmosphere, adding FeCl2 and FeCl3 into the mixed solution of sodium hydroxide aqueous solution and hydrochloric acid solution for reaction to obtain Fe3O4 nano-particles; and (2) reacting carboxymethyl-alpha-cyclodextrin with Fe3O4 nano-particles in PBS (Phosphate Buffer Solution), thereby obtaining the functionalized Fe3O4 nano-particles. The Fe3O4 nano-particle modified by alpha-cyclodextrin provided by the invention can simultaneously have good enrichment and purification abilities to trace phenylarsonic acid in complex samples; and meanwhile, the Fe3O4 nano-particle is superparamagnetic, so that centrifugation is not needed in sample processing, the pretreatment procedures of the sample are greatly simplified, the pretreatment time is shortened, and the pretreatment efficiency of the sample is improved.

The invention provides a nano-grade ZnO loaded magnetic biological carbon composite photocatalyst and a preparation method thereof. The preparation method comprises the following steps: step 1: carrying out thermal hydrolysis on a biomass under an alkaline condition to extract plant fibers; after drying, carrying out pre-carbonization treatment; mashing and sieving with a sieve with the size of 10to 20 meshes; step 2: mixing a ferrous salt solution and a potassium hydroxide solution; adding soluble zinc salt; step 3: adding the sieved plant fibers into the solution formed by step 2; uniformlymixing and adding a potassium nitrate solution; after sufficiently stirring, treating a mixture in a water bath; continuously stirring and ageing until black gel is formed, so as to obtain a black gel solution; step 4: after washing and drying the black gel solution, generating dry gel; calcining the dry gel and continuously introducing inert gas; after washing and drying, obtaining the nano-grade ZnO loaded magnetic biological carbon composite photocatalyst. According to the nano-grade ZnO loaded magnetic biological carbon composite photocatalyst, magnetic biological carbon is used as a carrier of the photocatalyst, and the problem that the photocatalyst in chromium-containing sewage treatment is difficult to separate and recycle is solved.