45 results about "Rat Bone Marrow" patented technology

Cellulose and sulfated cellulose fibrous meshes exhibiting robust structural and mechanical integrity in water were fabricated using a combination of electrospinning, thermal-mechanical annealing and chemical modifications. The sulfated fibrous mesh exhibited higher retention capacity for human recombinant bone morphogenetic protein-2 than the cellulose mesh, and the retained proteins remained biologically active for at least 7 days. The sulfated fibrous mesh also more readily supported the attachment and osteogenic differentiation of rat bone marrow stromal cells in the absence of osteogenic growth factors. These properties combined make the sulfated cellulose fibrous mesh a promising bone tissue engineering scaffold.

Intravenous administration of bone marrow cells collected from rat bone marrow or peripheral blood to a rat cerebral infarction model was found to be effective in treating cerebral infarction. Human and murine bone marrow stem cells showed similar effects. Mesenchymal cells such as bone marrow cells, cord blood cells, or peripheral blood cells can be used as agents for in vivo administration against cranial nerve diseases.

The invention discloses a method for alkaline phosphatase to induce gelation and biomimetic mineralization of silk protein solution. The invention introduces a small molecule polypeptide that is sensitive to ALP and has good biocompatibility and self-assembly properties as a gelling factor The precursor, which can remove the phosphate group on the molecule under the catalysis of ALP to generate NY, triggers supramolecular self-assembly, thereby synergistically inducing SF co-self-assembly, and finally leads to the rapid formation of SF hydrogel. The ALP encapsulated in the SF‑NY hydrogel network still maintains its catalytic activity and induces the formation of apatite minerals in the gel by catalyzing β‑glycerophosphate to release free phosphate ions. Due to the mild gelation process and the formation of apatite minerals in the gel matrix, the biomimetic mineralized SF gel could not only be used as a biomimetic scaffold to promote the adhesion of rat BMSCs in vitro, Proliferation and osteogenic differentiation, and can also promote natural healing of femoral defects in a rat model.

The invention discloses a magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 and a preparation method thereof. The method for preparing the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 comprises the steps of: firstly preparing hydrophobic monodisperse Fe3O4 nano-particles by adopting a chemical oil-phase high-temperature method, and modifying the surfaces of the hydrophobic Fe3O4 nano-particles to ensure that the hydrophobic Fe3O4 nano-particles are dispersed in a water phase; preparing luminescent CdTe quantum dots of which the surfaces are provided with carboxyl groups, and precipitating the luminescent CdTe quantum dots on the surfaces of the magnetic Fe3O4 nano-particles through the co-precipitation; then utilizing ligand exchange to modify a silane coupling agent on the surfaces of the luminescent CdTe quantum dots; and finally forming an outermost SiO2 coating layer through silane or silicon ester hydrolysis. The diameter of the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 is between 30 and 50nm; the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2has double functions of magnetism and fluorescence at the same time, has strong and durable fluorescence intensity after labeling rat bone marrow-derived mesenchymal stem cells, and apparently reduces cellular magnetic resonance signals. The particle has broad application prospect in the fields such as biological labeling, bioseparation and the like.

The invention provides a immortal stromal cell systme of rat's marrow and preparation thereof. Immortal stromal cell of rat's marrow can be obtained in short time by the inventive method. The inventive hTERT immortal stromal cell systme of rat's marrow is stable, can be sequentially passed through generations and keep diploid karyotype and characteristics of normal stromal cell of rat's marrow, has extremely strong proliferation ability, and realize immortalization. Immortal stromal cell system of rat's marrow established by the inventive method can conveniently and quickly obtain large amount of stromal cell of rat's marrow with diploid karyotype. This no doubt has great promotion to research of introduction and bone marrow stromal cell in vitro and in vivo.

The invention discloses a method for constructing a rat bone mesenchymal stem cells (BMSCs) cell strain for expressing c-met protein through regulation of doxycycline. The method comprises the steps that an rtTA gene lentiviral expression vector is constructed and subjected to transfection into BMSCs so that a BMSCs cell strain (a Tet-On Advanced BMSCs cell strain) for stably and efficiently expressing rtTA protein can be obtained; then, a Len-TRE min CMV-c-met / ALB-GFP lentiviral vector is constructed and subjected to transfection into the Tet-On Advanced BMSCs, and a flow cytometry is used for screening GFP positive BMSCs; and finally the GFP (+) Tet-on-c-met BMSCs cell strain is obtained. The doxycycline can be added into the obtained BMSCs cell strain to induce the obtained BMSCs to express the c-met protein, and the method has important significance for study on blood doping of the cell strain for treating hepatic failures, the directional homing and differentiation capacities of the BMSCs along with the HGF concentration and the dose-effect relationship between the capacities and the HGF / c-met axis expression level.

The invention discloses an SD rat-derived bone marrow mesenchymal stem cell induction medium and an induction method. The induction medium is composed of α-MEM medium and additives. In terms of final concentration, the additives are 5-100ng / ml Composition of transforming growth factor-alpha, 10-200ng / ml regulatory protein beta 1, 100-3000nM trehalose and 50-5000nM dibutyryl cyclic adenosine monophosphate. The SD rat-derived bone marrow mesenchymal stem cell induction medium and induction method of the present invention can significantly increase the secretion of BDNF, HGF, VEGF, GDNF and other factors of rat bone marrow mesenchymal stem cells, and the types of secreted factors are also relatively abundant and It can be secreted stably for a long time; the method of cytokine induction avoids the risks and uncertainties brought about by other methods such as genetic modification, and is more suitable for industrialization and clinical application.