51 results about "Cyclic adenosine monophosphate" patented technology

The invention relates to the technical field of diagnosis markers and diagnosis methods for mental diseases of children and adolescents, in particular to a plasma metabolism diagnosis marker and diagnosis system for depression of children and adolescents. The plasma metabolism diagnosis marker comprises at least one of cyclic adenosine monophosphate, methylpyridine, unsaturated undecylenyl carnitine, histidine, prolyl hydroxyproline, tryptophan and glucuronic acid. According to the scheme, non-target metabonomics data is analyzed, and seven candidate diagnostic markers for depression of children and adolescents are screened and determined from common differential metabolites. Then, through targeted metabonomics research, diagnostic markers and combinations thereof with ideal diagnostic effects are determined, and then a diagnostic model is constructed. And a sex factor is also incorporated into the diagnosis model, so that the diagnosis effect is further improved. According to the technical scheme, the technical problem that in the prior art, reliability and popularization potential of depression plasma metabolism biomarkers of children and adolescents are not ideal can be solved, and the depression plasma metabolism biomarkers have ideal popularization and application prospects.

The invention discloses an anti-aging composition derived from space breeding desert algae as well as a preparation method and application of the anti-aging composition, and relates to the technical field of application of desert algae. The anti-aging composition is an extract of Hassalliasp.P06 after space breeding, and the preservation number of the Hassalliasp.P06 is CCTCC (China Center for Type Culture Collection) M20242054, and the preservation number of the Hassalliasp.P06 is CCTCC M20242054. The anti-aging composition derived from the space breeding desert algae comprises the following components: polypeptide, free amino acid, polysaccharide and cyclic adenosine monophosphate. The skin care product has very strong moisturizing capacity, can promote the proliferation of skin fibroblasts and remarkably improve the skin compactness, has a soothing effect and oxidation resistance, and can be applied to skin care products or cosmetics to be used as a compactness agent or an anti-aging agent so as to improve the skin aging symptom.

The present invention relates to a composition for maturation of adrenal organoids, a method for maturation of adrenal organoids using same, and a method for transplantation of organoids prepared by the method. In the present invention, the structurally and functionally mature adrenal organoids prepared under optimized culture conditions using the composition for maturation of adrenal organoids containing adrenocortical hormone and cyclic adenosine monophosphate have been structurally and functionally improved, and it was confirmed that the adrenal organoid chip having a three-dimensional vascular network structure manufactured using the same actually replaces the function of the adrenal gland without side effects after transplantation into a mouse. Accordingly, the present invention can be advantageously used for transplantation or regenerative therapy, drug screening, drug responsiveness evaluation, and the like.

The invention discloses a construction method of an escherichia coli mutant for producing succinic acid by fermentation of a synthetic medium. The method comprises the following steps: firstly, knocking out a lactic dehydrogenase gene ldhA, a pyruvate formate lyase gene pflB, a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein, an ethanol dehydrogenase gene adhE, an acetokinase-phosphate transacetylase gene ackA-pta, and a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein in escherichia coli; a phosphoenolpyruvate carboxykinase gene pck from bacillus subtilis is integrated at an SS9 safety site of a strain to obtain escherichia coli ESC6 with high succinic acid yield; and mutating one or more loci in one or more genes of a glucose-transcriptional inhibition factor gene mlc, a nitrate response regulatory factor gene narL and a cyclic adenylate receptor protein gene crp to obtain the escherichia coli mutant capable of producing succinic acid by fermentation of a synthetic culture medium, wherein the one or more loci in one or more genes of the glucose-transcriptional inhibition factor gene mlc, the nitrate response regulatory factor gene narL and the cyclic adenylate receptor protein gene crp are mutated. The strain can be fermented in a synthetic medium to produce succinic acid, so that the fermentation cost is greatly reduced, and the strain has a very wide application prospect.

The invention belongs to the technical field of fungal keratitis medicines. The invention provides application of cyclic adenosine monophosphate and metal salt thereof in preparation of an external product for preventing or treating fungal keratitis, aiming at the problems of poor permeability, low solubility, low bioavailability and possibility of generating toxic and side effects on cornea after long-term use of a clinically common local antifungal drug for treating fungal keratitis. As an antifungal active molecule for external administration, the cAMP has good water solubility, corneal permeability, broad-spectrum antifungal activity and biological safety. Compared with a traditional antifungal drug, the cAMP can be efficiently delivered to a cornea infection part and directly acts on pathogenic fungi by virtue of excellent water solubility and cornea permeability, so that the toxic and side effects on cornea tissues are reduced, and meanwhile, the bioavailability is improved. The cAMP shows good antifungal activity when the pH (Potential of Hydrogen) value is in a range of 4 to 7.5.

Provided are a method of producing a human pluripotent stem cell-derived Leydig-like cell (Leydig-like cells) capable of secreting testosterone stably and persistently, and a human pluripotent stem cell-derived Leydig-like cell. Also provided is a human pluripotent stem cell-derived Leydig-like cell that enables long-term maintenance of a Leydig-like cell secreting a sufficient amount of testosterone, and is improved in efficiency of differentiation induction. The method includes the steps of: culturing a human pluripotent stem cell under a culture condition with addition of one or a plurality of kinds selected from the group consisting of: a WNT canonical pathway activator; bone morphogenetic protein 4 (BMP4); and vascular endothelial growth factor (VEGF); and forcedly expressing NR5A1, and is achieved by controlling the timing of addition and removal of cyclic adenosine monophosphate (cAMP), suspension culture, adhesion culture, and the like.

The utility model relates to the technical field of extraction equipment, and discloses production equipment for extracting cyclic adenosine monophosphate in red dates, which comprises a base, the top of the base is fixedly connected with a grinding box, the inner wall of the grinding box is fixedly connected with a plurality of auxiliary blocks, and the right side of the grinding box is fixedly connected with a servo motor II; and the output end of the second servo motor penetrates through the right side of the grinding box and is fixedly connected with a grinding roller, the bottom of the grinding box communicates with a feeding port, the right side of the interior of the base is fixedly connected with a water tank, and the bottom of the front side of the water tank communicates with a connecting pipe. According to the jujube extraction device, jujubes are well smashed through cooperation of the grinding rollers and the auxiliary blocks, the jujubes and water are fully mixed, the extraction process is very convenient, the jujubes in the water are stirred through the stirring blades, the water temperature in the separation barrel is controlled through the temperature detector, and the jujube extraction efficiency is improved. The cyclic adenosine monophosphate can be better separated from the jujubes.

The invention relates to preparation of a cyclic adenosine monophosphate (cAMP) detection kit by using a chemiluminescence immunoassay technology. Cyclic adenosine monophosphate is an important second messenger for cell signal transduction and has wide physiological functions. Cyclic adenosine monophosphate is small in molecular weight and poor in immunogenicity, so that when a corresponding antibody is prepared, a proper derivative site needs to be selected firstly, and a derived hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and strong affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of a cyclic adenosine monophosphate hapten, preparation of a cyclic adenosine monophosphate complete antigen and an anti-cyclic adenosine monophosphate antibody, a method for measuring the concentration of cyclic adenosine monophosphate, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise design and chemical synthesis of a cyclic adenylate derivative. The cyclic adenylic acid derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

The invention provides a beta-galactosidase compound and a method. The beta-galactosidase compound comprises beta-galactosidase and cyclic adenosine monophosphate combined with amino acid residues of the beta-galactosidase. According to the invention, small molecular cyclic adenosine monophosphate acts on lactase molecules, and the catalytic activity of lactase is significantly enhanced through unique intermolecular interaction. Compared with a traditional lifting means, the method has the advantages that the lifting efficiency is higher, an ideal lactase activity level can be achieved in a shorter time, and a powerful guarantee is provided for lactose digestion and utilization.

The invention relates to the technical field of nutritional health-care drinks, in particular to an FDP cyclic adenosine monophosphate compound drink composition with a myocardial protection effect. The formula comprises the following raw materials: 8 g of water, 2 g of a red date extracting solution, 0.15 g of 1, 6-fructose diphosphate trisodium salt, 0.1 g of deer myocardial peptide powder, 0.1 g of anhydrous citric acid, 0.004 g of potassium sorbate, 0.00003 g of vitamin B1, 0.00003 g of vitamin B2 and 0.00003 g of vitamin B6. The preparation method comprises the following steps: molecular docking; performing ultrasonic synergism; carrying out steady-state treatment; and mixing. The invention provides the FDP cyclic adenosine monophosphate compound drink composition with the myocardial protection effect, and the efficacy improvement and clinical application value of a compound myocardial protection product are improved.

The invention discloses an antihypertensive beverage formula and a preparation method thereof, and the antihypertensive beverage formula comprises the following components in parts by weight: 0.01-10 parts of saffron, 0.1-100 parts of sophora flower bud, 0.1-100 parts of huanglongcao, 0.1-100 parts of corn stigma, 0.1-100 parts of cassia occidentalis, 0.1-100 parts of white chrysanthemum and 0.1-100 parts of cistanche. According to the invention, the huanglongcao contains baicalin, baicalin, baicalein, scutellaria baicalensis enzyme and the like; the common selfheal fruit-spike contains triterpenoids, flavonoids, steroid glucoside and coumarin compounds, and the cassia seed contains anthraquinones and naphthopyrone compounds; when the components act in the human body, the CAMP (cyclic adenosine monophosphate) level of the human body is increased, so that the platelet accumulation is obviously reduced, the resistance of blood circulation is reduced, the pressure of blood on vascular walls is reduced, and the effects of reducing blood pressure, improving eyesight, inducing diuresis and inhibiting cholesterol synthesis are achieved, so that the purpose of reducing blood pressure is achieved.

The present invention demonstrates the functional enhancement of melatonin-primed stem cells through ten indicators including self-renewal ability, engraftment rate, anti-inflammatory ability, and immunosuppressive ability in a cell culture model. It has been identified that in the primed stem cells according to the present invention, the activity of glutathione (GSH) is increased, through signaling involving cyclic adenosine monophosphate (cAMP) response element-binding protein-1 (CREB1) and nuclear factor (erythroid-derived-2) like-2 (NRF2) as a molecular mechanism and, simultaneously, the expression of PD-L1 protein is increased by JAK1-STAT3 signaling. The stem cells primed according to the present invention have improved stemness, and thus can be effectively used in the treatment of inflammatory diseases, immune diseases and the like.

Provided are a method of producing a human pluripotent stem cell-derived Leydig-like cell (Leydig-like cells) capable of secreting testosterone stably and persistently, and a human pluripotent stem cell-derived Leydig-like cell. Also provided is a human pluripotent stem cell-derived Leydig-like cell that enables long-term maintenance of a Leydig-like cell secreting a sufficient amount of testosterone, and is improved in efficiency of differentiation induction. The method includes the steps of: culturing a human pluripotent stem cell under a culture condition with addition of one or a plurality of kinds selected from the group consisting of: a WNT canonical pathway activator; bone morphogenetic protein 4 (BMP4); and vascular endothelial growth factor (VEGF); and forcedly expressing NR5A1, and is achieved by controlling the timing of addition and removal of cyclic adenosine monophosphate (cAMP), suspension culture, adhesion culture, and the like.

The invention relates to a traditional Chinese medicine composition for treating ischemic heart failure and a preparation method thereof, and belongs to the field of traditional Chinese medicine pharmacy, and the traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 5-15 parts of cassia twig, 5-15 parts of bighead atractylodes rhizome, 5-15 parts of poria cocos, 6-12 parts of rhizoma zingiberis, 5-15 parts of acanthopanax, 10-30 parts of fructus polygoni orientalis, 3-9 parts of verbena, 6-12 parts of dogwood and 3-9 parts of honey-fried licorice root. The composition can remarkably reduce the level of N-terminal proB-type natriuretic peptide, remarkably improve pathological changes of heart tissues of model rats, regulate and control the heart functions of the model rats, improve the systolic function of the heart, relieve expression of inflammatory factors such as interleukin-1beta, interleukin-6 and tumor necrosis factor-alpha of the rats and improve the anti-inflammatory effect of the rats. The action mechanism of the compound relates to a plurality of pathways such as extracellular matrix receptor interaction, a chemotactic factor signal pathway, a relaxin signal pathway, a cyclic adenosine monophosphate signal pathway, a nuclear factor-kB signal pathway, fatty acid metabolism and a Toll-like receptor signal pathway, and the compound is an effective medicament for treating ischemic heart failure.

The application of a drug or medical aesthetic product containing tolvaptan as an active ingredient for inhibiting melanin synthesis. Cell activity detection and melanin content determination show that tolvaptan can significantly reduce the intracellular melanin content without affecting the cell activity. Tyrosinase activity detection shows that tolvaptan does not affect the catalytic activity of TYR. Western blotting experiment shows that tolvaptan can down-regulate the expression levels of TYR family proteins, microphthalmia-associated transcription factor (MITF), cyclic adenosine monophosphate response element binding protein (CREB) and melanocortin 1 receptor (MC1R).

A formulation of pentoxifylline and hesperidin for hemorrhoids and acute fissure [0005] The present invention discloses a formulation of pentoxifylline and hesperidin for hemorrhoids and acute fissure. The formulation comprises pentoxifylline at a percentage of 90%, hesperidin at a percentage of 10% along with excipients. The formulation of pentoxifylline and hesperidin aids in enhancing the flow of blood by decreasing the viscosity. The improved blood flow aids in alleviating the symptoms caused by hemorrhoids and acute fissure. The formulation inhibits the activity of phosphodiesterase aiding in increased production of cyclic adenosine monophosphate (cAMP) in the skeletal muscle leading to vasodilation. Vasodilation aids in decreased venous hypertension and enhanced blood flow in the rectal blood vessels. The formulation of pentoxifylline and hesperidin exhibits antioxidant and anti-inflammatory properties.

The invention provides a preparation method and application of red date paste with a neuroprotective peptide fragment. An innovative process capable of retaining bioactive substances of the red dates to the greatest extent is developed by deeply researching various pretreatment technologies such as air flash evaporation, ultrasonic light cooking and high-pressure cooking, the content of adenosine cyclophosphate in the red date paste prepared by the process is up to 370 + / -14 micrograms per gram, the protein content is up to 1.12%, and the red date paste is suitable for large-scale production. And a key peptide fragment with a neuroprotective effect is found in the red date polypeptide. In a PC12 cell model induced by hydrogen peroxide, Cyclo-GP and Ala-Pro-Ile show a remarkable cell protection effect, and the Cyclo-GP and Ala-Pro-Ile are two of the five candidate peptide fragments with the most remarkable neuroprotection function effects. The preparation process of the red date paste is simple and convenient to operate, high in extraction efficiency and low in cost, not only provides a new technical direction for deep processing of red dates, but also lays a solid foundation for developing red date products with a nerve protection function.

The invention relates to the technical field of prediction models, and particularly discloses a pain and rheumatism syndrome biomarker, a diagnosis / prediction model and a construction method thereof. The pain and rheumatism syndrome biomarker comprises at least one of acetyl vanillin, cyclic adenosine monophosphate, methyl vanillate and uridine succinic acid; the pain and rheumatism syndrome biomarker comprises a pain and rheumatism syndrome diagnosis marker and a pain and rheumatism syndrome recurrence prediction marker; the pain and rheumatism syndrome diagnosis marker comprises at least one of acetyl vanillin, cyclic adenosine monophosphate and methyl vanillate; the pain and rheumatism syndrome recurrence prediction marker comprises cyclic adenosine monophosphate and uridine succinic acid. According to the application, a diagnosis model of the pain and rheumatism syndrome can be constructed on the basis of the expression levels of the three plasma metabolites, namely the acetylvanillin, the cyclic adenosine monophosphate and the methyl vanillate; based on the plasma expression level of cyclic adenosine monophosphate and uridine succinic acid, a gout recurrence prediction model can be developed, and high prediction accuracy is achieved.

Nucleic acid molecules comprising a first sequence encoding a cyclic nucleotide gated potassium channel or a functional fragment thereof and a second sequence encoding a chemogenetically activatable cyclic adenosine monophosphate (cAMP) generating receptor or a functional fragment thereof are provided. Expression vectors comprising the molecules, fusion proteins encoded by the molecules, cells, kits and pharmaceutical compositions are also provided. Methods of hyperpolarizing a cell, depolarizing a cardiac cell and treating a disease or condition are also provided.

The present invention relates to the technical field of drug synthesis, and in particular to a method for synthesizing sodium dibutyryl adenosine cyclophosphate. The synthesis method comprises at least the following steps: sodium adenosine cyclophosphate reacts with butyryl halide under the action of a phase transfer catalyst to generate sodium dibutyryl adenosine cyclophosphate, the butyryl halide is selected from n-butyryl chloride and / or n-butyryl bromide, and the phase transfer catalyst is selected from cyclic crown ethers, quaternary ammonium salts, tertiary amines and / or quaternary phosphonium salts. The present invention uses butyryl halide instead of butyric anhydride to react with sodium adenosine cyclophosphate to prepare sodium dibutyryl adenosine cyclophosphate, thereby reducing the amount of acylating agent used, simplifying the post-processing process of the reaction, improving the yield, and enhancing the product quality and stability.

The application discloses a kind of serum-free epidermal melanocyte culture medium and its application, method includes by dissolving adenine, hepatocyte growth factor (HGF), glutamine, retinoic acid, vitamin E, nicotinic acid, sodium selenite, sodium hyaluronate and recombination human type XVII collagen are configured into initial culture medium;Preparation of melanocyte stimulating hormone, endothelin-3, transferrin, triiodothyronine, cholesterol, neuroregulin 1 (NRG1), 3-isobutyl-1-methyl purine (IBMX), dibutyryl cyclic adenosine monophosphate (db-cAMP), cholera toxin, hydrocortisone and recombination human insulin mother liquor;Step three: according to culture and concentration, a certain amount of mother liquor of step two is added to initial culture medium.The culture medium can effectively promote human epidermal melanocyte proliferation, melanin accumulation and help to promote human umbilical cord mesenchymal stem cells into human epidermal melanocyte, provide key advantage for obtaining functional melanocyte with high activity and therapeutic potential.

The invention relates to an application of kunbutton alcohol as an active ingredient in preparation of a medicine or a medical beauty product for inhibiting melanin synthesis. Cell activity detection and melanin content determination show that the content of melanin in cells can be remarkably reduced under the condition of not influencing the cell activity. Tyrosinase activity detection shows that tolvaptan does not affect the catalytic activity of TYR. Protein immunoblotting experiments show that tolvaptan can down-regulate the expression levels of TYR family proteins, ommatidium-associated transcription factors (MITF), cyclic adenylate reaction element binding proteins (CREB) and melanocortin-1 receptors (MC1R).

The present disclosure is directed to methods (e.g., in vitro methods) for detecting the presence of neutralizing antibodies to PTH or PTHrP analog in a sample. The in vitro method comprises the steps of obtaining a sample from a subject; contacting the sample with a cell; measuring cyclic adenosine monophosphate (cAMP) levels; and detecting the presence of neutralizing antibodies, when cAMP levels are reduced relative to a negative control sample without neutralizing antibodies. An in vitro method of detecting the presence of neutralizing antibodies in a sample from a subject treated with Abaloparatide is also provided. Further provided herein is a kit for carrying out the methods described herein comprising components required to carry out the obtaining, contacting, measuring and detecting steps and instructions for use.

The invention relates to the technical field of nutritional health-care drinks, in particular to FDP cyclic adenosine monophosphate compound oral liquid and a production process thereof. The invention relates to an FDP cyclic adenosine monophosphate compound oral liquid, which is prepared from the following raw materials by mass: 8 g of water, 2 g of a red date extract, 0.15 g of 1, 6-fructose diphosphate trisodium salt, 0.1 g of deer myocardial peptide powder, 0.1 g of anhydrous citric acid, 0.004 g of potassium sorbate, 0.00003 g of vitamin B1, 0.00003 g of vitamin B2, and 0.00003 g of vitamin B6. The production process of the FDP cyclic adenosine monophosphate compound oral liquid comprises the following steps: dynamic enzymolysis; coupling processing; carrying out nanometer stabilization; and filling at low temperature. According to the FDP cyclic adenosine monophosphate compound oral liquid and the production process thereof, the industrialization process of the compound myocardial protection oral liquid is improved, and the clinical application value of the compound myocardial protection oral liquid is increased.

The invention belongs to the technical field of medicine, and relates to a gold nanocluster and a preparation method and application thereof, the ligand of the gold nanocluster is cyclic adenosine monophosphate, the gold nanocluster emits blue-green fluorescence under an ultraviolet lamp, and the fluorescence excitation spectrum and the emission spectrum of the gold nanocluster are 376 nm and 482 nm respectively. The gold nanocluster is synthesized by taking cell second messenger cyclic adenosine monophosphate (cAMP) as a functional ligand, the size of the gold nanocluster is 1-2nm, the gold nanocluster emits blue-green fluorescence under an ultraviolet lamp, and the fluorescence excitation spectrum and the emission spectrum of the gold nanocluster are 376nm and 482nm respectively. The traditional Chinese medicine composition can relieve liver cell inflammation and improve liver glycometabolism and an insulin signal channel, so that the traditional Chinese medicine composition has a good effect of improving the diabetes mellitus liver insulin resistance, can be used for preparing the medicine for improving the diabetes mellitus liver insulin resistance and has a good application prospect.

The invention belongs to the technical field of medicinal and edible foods, and discloses an application of a red date extract containing cyclic adenosine monophosphate in preparation of a food for improving premature ovarian failure. The high-content cAMP in the red date extract is used as a core efficacy carrier, and animal experiments prove that the cAMP can accurately act on the pathologic link of premature ovarian failure. The limitation of single conditioning is broken through, and the dual effects of ovarian function improvement and qi and blood conditioning are achieved. The cAMP in the red date extract is combined with the nourishing effects of the Chinese wolfberry fruits, the rhizoma polygonati and the like, so that the premature ovarian failure can be improved in a targeted manner, and anemia can be effectively corrected. Through high-activity component targeting, multi-effect synergistic conditioning and safe process guarantee, side effects and dependency of western medicines are avoided, technical shortages of traditional products are made up, a safe, efficient and accurate conditioning scheme is provided for women with premature ovarian failure, clinical application value and industrial transformation potential are both achieved, and the pharmaceutical composition is worthy of popularization and application. The policy orientation of high-quality development of medicinal and edible functional foods is met.

Provided is a composition that includes an extract from seaweed and has excellent action and efficacy. This composition is characterized by including extracellular vesicles (EVs) and carotenoids.

The invention discloses a fluorescent probe for cAMP transport dynamic visual detection and efficient inhibitor screening, the fluorescent probe comprises cAMP as a targeting unit and jatrorrhizine hydrochloride as a fluorophore, and the jatrorrhizine hydrochloride and the cAMP are connected through flexible molecules; the fluorescent probe has good water solubility and aggregation-induced emission (AIE) characteristics, has high sensitivity and stability, can detect the process of transmembrane protein transport cAMP (cyclic adenosine monophosphate) in real time, rapidly and highly sensitively, and is especially suitable for the SLCO4C1 membrane protein mediated cAMP (cyclic adenosine monophosphate) transport process, thereby realizing visual fluorescence monitoring of the transport process. And meanwhile, the method can also be used for screening proteins interacting with cAMP, such as a PDE protein inhibitor.

The invention relates to the technical field of component detection, in particular to a method for detecting insomnia treatment components of semen ziziphi spinosae with shells, and the method comprises the steps of component identification, insomnia component research and experimental result analysis. Comprising the following steps: carrying out comprehensive characterization on chemical components of shell spina date seed powder by adopting an ultra-high performance liquid chromatography-quadrupole rod-electrostatic field orbitrap coupling technology to obtain a total ion chromatogram; the method comprises the following steps: constructing a wild jujube chemical component information database according to a TCMSP database, a Chemspider database, a PubChem database and related literature data; by utilizing Xcalibur software, the network diagram drawn by the method visually shows the incidence relation between different components in the spina date seeds and anti-insomnia targets and diseases, possible main active components such as cyclic adenosine monophosphate and lysimachimine are determined through network topology analysis, and a clear active component clue is provided for research and development of medicines based on the spina date seeds.

The invention discloses a neural stem cell culture medium which is composed of an Rho kinase inhibitor Y-27632, an HDAC inhibitor sodium valproate, an E-cadherin regulator 8-bromocyclic adenylate, a Wnt signal channel regulator CHIR99021, an anti-aggregation agent polyether F-68, heparin and an NSC complete culture medium. The neural stem cell culture medium is applied to passive control of the size of the neural stem cell aggregate. The invention further discloses a passive control method for the size of the neural stem cell aggregate. The neural stem cell culture medium provided by the invention can effectively and accurately control the size of the cell aggregate, improves the consistency and the cell viability of the cell aggregate under the condition of not influencing the physiological characteristics of cells, avoids the center necrosis problem of the aggregate, provides a more economical and efficient scheme for large-scale culture of neural stem cells, and has wide application prospects. And the application prospects in the fields of cell therapy, drug screening and the like are expanded.