3 results about "Cyclic adenosine monophosphate" patented technology

The application discloses a kind of serum-free epidermal melanocyte culture medium and its application, method includes by dissolving adenine, hepatocyte growth factor (HGF), glutamine, retinoic acid, vitamin E, nicotinic acid, sodium selenite, sodium hyaluronate and recombination human type XVII collagen are configured into initial culture medium;Preparation of melanocyte stimulating hormone, endothelin-3, transferrin, triiodothyronine, cholesterol, neuroregulin 1 (NRG1), 3-isobutyl-1-methyl purine (IBMX), dibutyryl cyclic adenosine monophosphate (db-cAMP), cholera toxin, hydrocortisone and recombination human insulin mother liquor;Step three: according to culture and concentration, a certain amount of mother liquor of step two is added to initial culture medium.The culture medium can effectively promote human epidermal melanocyte proliferation, melanin accumulation and help to promote human umbilical cord mesenchymal stem cells into human epidermal melanocyte, provide key advantage for obtaining functional melanocyte with high activity and therapeutic potential.

The application relates to the field of biological technology, in particular to application of cyclic adenosine monophosphate in promoting biomass increase of pasture and root nodule, wherein the cyclic adenosine monophosphate significantly improves the fresh weight biomass of the above-ground part, the root part and the single-plant root system root nodule of Medicago truncatula inoculated with rhizobium, and the difference is significant.

This invention discloses a method for preparing a molecularly imprinted electrochemical sensor for detecting cyclic adenosine monophosphate (cAMP). The sensor uses reduced graphene oxide (RGO) and gold nanoparticles as modifying materials. RGO is drop-coated onto the surface of a glassy carbon electrode and dried to obtain a modified electrode RGO / GCE. The RGO / GCE is then placed in a sulfuric acid deposition solution containing chloroauric acid, and gold nanoparticles are used to modify the RGO / GCE surface using a potentiostatic method to form a gold substrate, resulting in a deposited electrode AuNPs / RGO / GCE. The AuNPs / RGO / GCE is then electropolymerized in an electrolyte polymerization solution containing the template substance cAMP and the functional monomer o-phenylenediamine to obtain a dense and insulating polymer film. The polymerized electrode is immersed in an elution solution and eluted using cyclic voltammetry to obtain MIP / AuNPs / RGO / GCE. This sensor exhibits specific recognition selectivity for cAMP, high detection sensitivity, low detection limit, good reusability, and stability.