216 results about "Cyclic adenosine monophosphate" patented technology

The present invention is directed to the use of a PDE4 inhibitor and a glucocorticoid to treat peripheral B-cell neoplasms. In particular, the present invention provides a method of treating individuals (e.g. patients) diagnosed with peripheral B-cell leukemias by administering pharmaceutical compositions comprising Type 4 cyclic adenosine monophosphate phosphodiesterase inhibitors and a glucocorticoid. Preferably, the combination of the PDE4 inhibitor and the glucocorticoid has a synergistic effect on apoptosis such that the level of apoptosis induced is greater than the level that would be expected by simply adding a PDE4 inhibitor to a glucocorticoid.

The present invention relates to methods of inducing ovulation in a female host comprising the administration of a non-polypeptide cyclic adenosine monophosphate (cAMP) level modulator to female host. In another aspect, the invention provides for specific administration of the phosphodiesterase inhibitor prior to the luteal phase of the host's ovulatory cycle. Preferred non-polypeptide cAMP level modulator include phosphodiesterase inhibitors, particularly inhibitors of phosphodiesterase 4 isoforms.

The invention discloses a method and application for inducing human umbilical cord mesenchyme stem cells to be differentiated into testicular interstitial cells. The method comprises the following step of culturing human umbilical cord mesenchyme stem cells of patients suffering from adenovirus and carrying mice steroidogenic factor-1 genes in a DMEM-F12 culture solution containing 0.3-3ng / ml of luteinizing hormone, 200-800mu M of dibutyryl cyclic adenosine monophosphate, 5*10<-6>-5*10<-4>M of all-trans retinoic acid (ATRA), 10mU / ml of human chorionic gonadotropin and 2.4uM of adrenocorticotrophic hormone for a week. Induced by the method in the invention, the human umbilical cord mesenchyme stem cells can be differentiated into testicular interstitial cells in vitro and provides important sources of cells for treating testosterone shortage by the cell replacing method or the genetic method.

The invention provides arthrobacter for producing cyclic adenosine monophosphate through fermentation and application thereof. With a preservation number of CGMCC No.3584, the arthrobacter of the invention has a A302 strain which is three times higher than an original strain in terms of the cyclic adenosine monophosphate output. Undergoing over 10 generations of passage, the A302 strain of the invention is stable in producing cyclic adenosine monophosphate.

The present invention provides a fully nutritional formula product with special medical purposes for liver diseases. The fully nutritional formula product is characterized by comprising the following materials in proportions: pre-digested carbohydrates, compound proteins, compound fats, medicinally and edibly homologous biological resources, dietary fibers, mineral premixes, vitamin premixes, moringa oleifera powder, cyclic adenosine monophosphate, etc. The product combines the metabolic characteristics and the nutritional requirements of patients with the liver diseases, and specially the medicinally and edibly homologous biological resource ingredients beneficial to the patients with the liver diseases are added. The product can improve the malnutrition status of the patients with the liver diseases, protects liver cells and promotes the repair and regeneration of the liver cells. Besides, the product is good in palatability, convenient for combination and uses, and easy for the patients with the liver disease to accept.

Cosmetic or pharmaceutical compositions and methods employ peptides of general formula (I):R1-AA1-AA2-AA3-R2,  (I)where AA1 is selected from -Tyr- and -Phe-, AA2 is -Tyr-, and AA3 is selected from -Nle- and -Met-,its stereoisomers, mixtures thereof and / or their cosmetically or pharmaceutically acceptable salts, in the treatment and / or care of conditions, disorders and / or diseases of the skin and / or hair by stimulating cyclic adenosine monophosphate synthesis (cAMP).

Compositions for addressing one or more of the effects of aging are described. The compositions comprise a first component comprising repair system activator(s) such as nicotinamide adenine dinucleotide (NAD+), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine mononucleotide (NaMN), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid riboside (NAR), 1-methylnicotinamide (MNM), cyclic adenosine monophosphate (cAMP) and combinations thereof; a second component comprising methyl donor(s), such as S-5'-adenosyl-L-methionine (SAM), methionine, betaine, choline, folate, vitamin B12, or combinations thereof; and a third component comprising antioxidant defense activators, such as H2O2, N2S, NaSH, Na2S, and several others, including combinations thereof.Methods of administering the disclosed compositions or separate formulations of repair system activator, methyl donors, and antioxidant defense activators are also disclosed.

The invention relates to the technical field of feed additives for livestock, and particularly relates to a compound feed additive for improving pork quality. The compound feed additive is prepared by mixing Chinese medicinal herb superfine powder with tea polyphenols, wherein the weight ratio of the Chinese medicinal herb superfine powder to the tea polyphenols is 2:1, the applied additive accounts for 0.75% of the total weight of the feed, and the Chinese medicinal herb superfine powder is 200-mesh superfine powder which is prepared by mixing the following Chinese medicinal herbs in parts by weight: 3 parts of astragalus root, 3 parts of bighead atractylodes rhizome, 2 parts of amomum fruit, 2 parts of dried tangerine peel, 2 parts of anisum fruit, 1 part of wrinkled gianthyssop herb, 2parts of rhizoma kaempferiae, 2 parts of cortex cinnamomi, 1 part of pine needle and 1 part of licorice root. The effective ingredients of the Chinese medicinal herbs and the tea polyphenols in tea leaves both can inhibit the activity of phosphodiesterase in cells, thereby increasing the cyclic adenosine monophosphate content of pig cells; and under the action of the cyclic adenosine monophosphate, the energy, fat and proteins in pigs are redistributed and are finally represented by the improvement of pork quality.

The invention relates to a method for extracting cyclic adenosine monophosphate (cAMP) from red jujubes, which comprises the steps of cleaning of the red jujubes, soaking of the red jujubes at normal temperature, precooking and pulping, zymohydrolysis on pectin mixed enzyme, filter pressing, anion resin combination, formic acid elution, nano-filtration and interception of formic acid with nano-filtration membrane, and purification, thus obtaining a finished product. The method solves the problems of complex current process, long period, low product purity and the like, and has the advantages of rich raw material sources, simple process, large processing amount, high extraction rate, high product purity and the like; the product is a natural non-toxic product; the finished product cAMP can be used for medical raw materials or used as food additive; the extracted byproduct red jujube juice can be continuously and deeply processed to promote the industrial chain of comprehensive development and achieve pollution-free production; the modern nano-filtration technology is applied to industrial production, therefore, not only the cost is reduced, but also a better path for deeply processing the red jujube raw material is provided, and the consumption of energy is greatly reduced; and the method not only has important economic benefit, but also has remarkable social benefits.

The invention provides a technique extracting cyclic adenosine monophosphate (cAMP) from Chinese dates. The Chinese dates are diacolated by water or ethanol solution or heated to extract; the extracting solution is connected to a resin column with macroporous adsorptive resins (OU-1 adsorptive resin) to absorb and is washed by the ethanol water or desorbed by acid water; the pH value of the stripping liquid is adjusted by sodium hydroxide and the stripping liquid is connected to the macroporous adsorptive resin column, and is desorbed by the ethanol with certain desorption after rinsing. The Chinese dates extractive product containing cAMP more than 1 per cent is gained after condensing and drying the stripping liquid. The invention is simple in technology, good in product quality, easy in preparation, high in extraction yield, free from three wastes and environment pollution, abundant in raw material source, low in cost and great in economic benefits.

The invention discloses a method for extracting cyclic adenosine monophosphate from dates, which comprises: pulverizing dates, adding water, mixing into pulp, microwaving the data pulp under the microwave power of 150-900 W for 10-450 seconds, microwaving the data pulp at 20-100 DEG C under the microwave power of 100-250 W for 10-60 minutes, carrying out the solid-liquid separation on the data pulp, and concentrating the separated liquid to obtain the concentrated cyclic adenosine monophosphate. The method enables all points in the data pulp to get the same action by utilizing the high-energyfunction of microwaves and the cavitation of the ultrasonic vibrations, so as to reduce the bonding force of the cyclic adenosine monophosphate and the data substrate, shorten the extraction time andincrease the extraction rate. In the extraction process, water is used as an extractant and no harmful chemical reagent for assistant extraction is added, so that the method can not cause the environmental pollution. Meanwhile, the method has the advantages of simple extraction technique and high extraction rate, and is suitable for industrial production.

The present invention relates to methods of inducing ovulation in a female host comprising the administration of a non-polypeptide cyclic adenosine monophosphate (cAMP) level modulator to the female host. In another aspect, the invention provides for specific administration of the phosphodiesterase inhibitor prior to the luteal phase of the host's ovulatory cycle. Preferred non-polypeptide cAMP level modulator include phosphodiesterase inhibitors, particularly inhibitors of phosphodiesterase type 4.

The present invention provides a method of preparing cyclic adenosine monophosphate, which includes the use of Arthrobacter sp. as an engineering bacterium, and the addition of precursors, inhibitors of glycolytic pathway and / or activators of tricarboxylic acid cycle into a fermentation medium, so as to acquire the cyclic adenosine monophosphate by fermentation. Further provided is a use of precursors, inhibitors of glycolytic pathway and / or activators of tricarboxylic acid cycle for preparing cyclic adenosine monophosphate by Arthrobacter sp.. The present invention inhibits the excessive glycolysis, increases the flux of pentose phosphate pathway, and enhances the tricarboxylic acid cycle by the addition of precursors, inhibitors of glycolytic pathway and / or activators of tricarboxylic acid cycle, so as to redistribute rationally the metabolic flux of central metabolic pathways, make the carbon flux more efficiently direct to the target product - cyclic adenosine monophosphate, and synthesize cyclic adenosine monophosphate by using carbon source efficiently.

The invention discloses a preparation process of red date condensed juice high in cAMP content. The preparation process includes the following steps of firstly, conducting separating, washing and smashing; secondly, conducting hot water extraction; thirdly, conducting cooling and heat preservation enzymic method oriented conversion; fourthly, adding composite enzyme preparations; fifthly, inactivating enzymes; sixthly, conducting centrifugation; seventhly, conducting filtration; eighthly, conducting concentration and pasteurization; ninthly, conducting filling. Compared with the prior art, the preparation process has the advantages that the process is simple, the production period is shortened, and the energy loss is reduced; extraction is conducted after smashing is conducted, and therefore extraction of nutritional ingredients in red dates can be improved; by means of the extraction and enzymolysis combined method, the juice yield is greatly improved, the processing time is greatly shortened, and the cyclic adenosine monophosphate content in red date juice is improved; date peel is utilized, and the cAMP content is improved; preparations of pectinase, cellulose and protease are used in a composite mode, and the hydrolysis capacity of enzymic preparations is improved; the step-by-step enzymolysis and extraction process is adopted, and interference between related enzyme preparations is avoided.

The invention discloses a composition for promoting hair growth, comprising tetrandrine, cyclic adenosine monophosphate, fullerol and panthenol, as well as the application of the composition for promoting the hair growth in the preparation of a hair growth promoting product. By means of the composition for promoting the hair growth, hair follicles can be effectively activated, an intercellular information path is strengthened, DHT is prevented from attacking the hair follicles, the hair follicle nipple activity is enhanced, the hair follicle cell proliferation is promoted, and the hair loss isprevented and treated. Human and animal keratin genes and collagen gene expressions are promoted to generate more keratins and collagens, the hair growth and the nutrient supplementation are promoted, and the effects of hair fixing, hair nourishing and hair care are achieved. The number, density, length, gloss, black degree, consistency, firmness and stoutness of hair can be quickly increased.

The invention relates to an adenosine monophosphate meglumine injection and a preparation method thereof. The preparation process is to take an appropriate amount of water for injection, add sodium chloride, cyclic adenosine monophosphate, and meglumine, stir to dissolve completely, add 0.05-0.2% (W / V) activated carbon for needles by volume, and stir for 15-30 minutes. Filter to remove carbon, add water for injection to nearly full amount, use phosphate buffer to adjust the pH value to between 6.0 and 6.5, add water for injection to the full amount, and check that the semi-finished product is qualified, filter, potting (full nitrogen gas in the whole process), and extinguish Bacteria, light inspection, packaging. The method has the advantages of selecting appropriate solvents and additives, improving the solubility and stability of meglumine cyclic adenosine monophosphate, and adopting a terminal sterilization preparation method to effectively ensure the sterility assurance level of the drug. The invention has the characteristics of formula composition, simple process, low production cost, strong drug stability and safety, and the like.

The invention discloses a method for extracting cyclic adenosine monophosphate from a fermentation liquid, and belongs to the technical field of biological active substance extraction. The method comprises the following steps: heating the fermentation liquid, and filtering the fermentation liquid through a membrane to obtain an ultra-filtrate; adjusting the pH of the ultra-filtrate to be alkaline, and enabling the ultra-filtrate to pass through a carbon chromatography column for adsorbing; eluting by using ammonia water; collecting an eluting solution; adjusting the pH of the eluting solution to be alkaline; vacuumizing at 55 to 60 DEG C and performing suction filtration; adjusting the pH of the filtrate to be acidic; adding methyl alcohol in a flowing manner for crystallizing; drying crystals in vacuum to obtain crude cyclic adenosine monophosphate; re-crystallizing the crude cyclic adenosine monophosphate to obtain finished cyclic adenosine monophosphate. The cyclic adenosine monophosphate prepared by the method is stable in quality; the purity is up to 99 percent; the yield is up to 75 percent; a traditional ion exchange and resin chromatography process is replaced by adopting a carbon chromatographic method; the production cost is reduced; ammonia water eluting is favorable to treat wastewater.

The invention provides mixed type drink which takes Chinese yam and Chinese date as main raw materials, wherein Chinese date is rich in nutrient components such as protein, fat, sugar, carotene, vitamin B, vitamin C, vitamin P, calcium, phosphorus, Ferrum, cyclic adenosine monophosphate and the like; and Chinese yam contains amylase, polyphenol oxidase and other substances, is beneficial to spleen and stomach digestion and absorption function, also contains various nutrients, has functions of enhancing body and nourishing kidney and replenishing essence, is suitable for the weaker after illness, and improves human immune. The Chinese yam-Chinese date juice drink comprises the following raw materials in parts by weight: 180-220 parts of Chinese yam, 45-55 parts of Chinese date, 90-110 parts of xylitol, 1-3 parts of a stabilizer, 0.5-1.2 parts of an antioxidant and 630-700 parts of water. The drink prepared by the technical scheme has high nutrient components and good taste; and two raw materials are mixed to meet the need of health preservation for people and achieve mutual promotion and complementation.

The invention discloses recombinant Escherichia coli capable of highly producing cyclic adenosine monophosphate. The systematic nomenclature is Escherichia coli; and the collection number is CGMCC No.4706. The invention also discloses application of the recombinant Escherichia coli capable of highly producing the cyclic adenosine monophosphate to the production of the cyclic adenosine monophosphate. In the invention, an adenylate cyclase gene which is obtained by cloning a cAMP producing strain shows high catalytic activity and stability in the whole-cell catalysis process of recombinant bacteria, and ensures that the conversion rate of a substrate, namely adenosine triphosphate (ATP) reaches over 90 percent; and the reaction system is simple, conditions are mild, the period is short, few byproducts are generated, and the method is clean and pollution-free and is a simple, quick and efficient production way.

The invention provides a method for fermentation production of cyclic adenosine monophosphate by controlling the dissolved oxygen content at two phases. The method adopts the two-phase dissolved oxygen content control manner to carry out batch fermentation in order to produce the cyclic adenosine monophosphate, fermentation strains are Arthrobacter sp having a preservation number of CGMCC No.3584, the dissolved oxygen in a thallus growth phase is 20-30%, and the dissolved oxygen is 5-10% after the thallus amount stabilizes. The method provided by the invention has the advantages of substantial improvement of the output of the cyclic adenosine monophosphate, no need of the addition of extra equipment in the fermentation process, saving of the electric energy for stirring and air supply in the later fermentation phase, and suitableness for industrialized production.

The invention provides a high-content jujube cyclic adenosine monophosphate extract and a preparation method thereof. The concentration of cyclic adenosine monophosphate in the extract is more than 15mg / 100g, solid or semi-solid jujubes are taken as raw materials, and the preparation method comprises the steps of extracting by a continuous low-temperature extractor with high pressure difference, concentrating an extraction solution, performing vacuum drying and crushing to obtain a jujube crude extract; extracting the crude extract by adopting a supercritical extraction technology to obtain the jujube cyclic adenosine monophosphate extract. The jujube cyclic adenosine monophosphate extract has prominent effects in the aspects of hypoxia resistance, fatigue resistance and improvement of life quality. The preparation method has the following obvious advantages: (1) the extraction transfer rate is high; (2) the production efficiency is high; (3) the quality controllability is high; (4) the operation controllable degree is high; (5) the comprehensive utilization rate of resources is high; (6) the comprehensive operation cost is low; (7) the degree of automation is high; (8) the environmental pollution is little.

The invention relates to a method for extracting red date cyclic adenosine monophosphate through an ultrasonic composite membrane technology. The method solves the problem that the existing extraction technology has the defects of complex processes, low extraction rate, high industrialization difficulty and low product purity. The method comprises the following steps of removing date pits of cleaned red dates, crushing the red dates obtained by the previous step, adding an appropriate amount of an ethanol solvent into the crushed red dates, carrying out ultrasonic reinforcement extraction, repeatedly carrying out ultrasonic extraction of extracted filter residues, merging the extract obtained by the previous step, removing macromolecules of bacteria, proteins, pectin, polysaccharides and starch, and monosaccharides of glucose and fructose respectively through ultrafiltration and nanofiltration, intercepting cyclic adenosine monophosphate-rich concentrate, and carrying out pressure reduction condensation and spray drying to obtain 30% of a powdery cyclic adenosine monophosphate crude product. The method provided by the invention is easy for operation, is convenient for extraction, is fast, has short extraction time and a high cyclic adenosine monophosphate extraction rate, and is suitable for industrialized production. A product obtained by the method has high purity, is purely natural and does not produce chemical pollution. Therefore, the method provides an effective approach for comprehensive development of red date further processing.

The invention discloses a preparation method and application of a cigarette spina date seed extract. The method comprises the following steps: obtaining a mixed bacteria liquid by mixing a Raoultella planticola seed culture solution with a Bacillus methylotrophicus VJ4-1 seed culture solution; inoculating the mixed bacteria liquid to a mixed extract, and obtaining a fragmentation broth through fragmentation; and performing deposition pumping filtration on the fragmentation broth, performing pressure-reduction concentration on a filtrate, and removing a solvent so as to prepare the cigarette spina date seed extract with relative density of 1.20 to 1.36. According to the invention, the mixed extract composed of a spine date seed extract, a grifola frondosus extract and a lychee seed extract is taken as the only carbon source, a nitrogen source and a substrate, alkaloid, triterpenes, flavonoids, seventeen amino acids and the like are included, ferulic acid, vitamin C, plant sterol, cyclic adenosine monophosphate and the like are also included, compared to a single carbon source, a microbial enzyme system is more easily utilized for fermentation and fragrance production, the source is wide and easily available, and the method and application also have the advantages of naturalness, environmental protection and zero harm.

The invention provides a method for continuously separating cyclic adenosine monophosphate. The method uses a continuous ion exchange system, and the continuous ion exchange system includes 5-80 Anion-exchange columns in series; the method includes continuously pumping the solution containing cyclic adenosine monophosphate into the anion-exchange column located in the adsorption area for adsorption, so that the adsorbed anion-exchange column passes through the impurity washing area, the elution area and the regeneration area in sequence. zone, and then return to the adsorption zone for adsorption again; the method also includes desalting, crystallizing, and drying the eluent flowing out of the elution zone to obtain cyclic adenosine monophosphate. The separation method of the present invention is simple and easy to operate, and has good effect. Not only its equipment investment and operation cost are low, but also the separation from kilogram level to ton level can be realized according to the amount of separation. All aspects have achieved excellent results, ensuring product quality.

The invention provides a gene-encoded cAMP fluorescence probe containing a fluorescence protein sequence or derivatives thereof and a polypeptide sensitive to cAMP. The polypeptide sensitive to the cAMP comprises (1) a CNBD structural domain with cAMP binding characteristics and / or is derived from family proteins of a guanine nucleotide exchange factor Epac sensitive to the cAMP and directly activated with the cAMP. The invention also discloses a preparing method of the gene-encoded cAMP fluorescence probe. The fluorescence probe is applied in cAMP detection, can detect the cAMP in the physiological level and the subcellsular level, and is excellent in specificity, visibility and sensitivity.

The invention discloses a crystallization method of cyclic adenosine monophosphate. The crystallization method comprises the following steps: adding an organic solvent used as an anti-solvent into 10-400g / L of a cyclic adenosine sodium phosphate aqueous solution at pH of 7.0-9.0 under the condition of stirring, carrying out pumping filtration after crystallization is completely finished, washing crystals by the use of ethanol, and carrying out vacuum drying to obtain cyclic adenosine sodium phosphate crystals; dissolving the above cyclic adenosine sodium phosphate crystals in water to obtain a saturated solution of the cyclic adenosine sodium phosphate crystals, adjusting pH of the solution to 4.0-5.5 by the use of acid while stirring, carrying out crystal growing for 0.5-8h, adjusting pH to 1.0-2.5, carrying out pumping filtration after crystallization is completely finished, washing crystals by the use of ethanol, and carrying out vacuum drying to obtain cyclic adenosine monophosphate crystals. By the adoption of the method provided by the invention, quality of the final product is obviously improved, yield of the crystal product is stably raised, and granular size of the crystals can be controlled by changing crystallization stirring speed or temperature. The method provided by the invention is simple to operate, has good repeatability, and is suitable for industrial production of cyclic adenosine sodium phosphate.

The invention discloses a serum-free immune cell culture medium. The serum-free immune cell culture medium is prepared mainly from amino acids, vitamins, minerals, D-glucose, 4-hydroxyethyl piperazinyl ethanesulfonic acid, insulin, phenol red, sodium pyruvate and transferrin. The invention discloses another serum-free immune cell culture medium which further comprises cofactors including salidroside, luteolin and resveratrol. The invention discloses another serum-free immune cell culture medium which further comprises activators including an acetyl coenzyme A, a coenzyme Q10, glucose 6-phosphate and cyclic adenosine monophosphate. A part of embodiments of the invention can increase the positive ratio of cell phenotypes.

The invention relates to a method for separating cyclic adenosine monophosphate, which comprises: absorbing solution of cyclic adenosine monophosphate by anion exchange column; purifying the anion exchange column absorbing the solution of cyclic adenosine monophosphate with alkaline solution serving as a purifying agent; and eluting the anion exchange column by using an acid solution as an eluting agent. The method provided by the invention separates 3',5'-cyclic adenosine monophosphate by using a novel anionite; and compared with the conventional method which separates 3',5'-cyclic adenosine monophosphate by using cation exchange resin, the method provided by the invention has the advantages of high absorption capacity, simple subsequent eluting process and high product yield. When the method is used for the industrial production of 3',5'-cyclic adenosine monophosphate, the separation and purification processes are simple, the production period is short, and the consumption resin is small.