9 results about "Cyclic adenosine monophosphate" patented technology

The invention relates to a traditional Chinese medicine composition for treating ischemic heart failure and a preparation method thereof, and belongs to the field of traditional Chinese medicine pharmacy, and the traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 5-15 parts of cassia twig, 5-15 parts of bighead atractylodes rhizome, 5-15 parts of poria cocos, 6-12 parts of rhizoma zingiberis, 5-15 parts of acanthopanax, 10-30 parts of fructus polygoni orientalis, 3-9 parts of verbena, 6-12 parts of dogwood and 3-9 parts of honey-fried licorice root. The composition can remarkably reduce the level of N-terminal proB-type natriuretic peptide, remarkably improve pathological changes of heart tissues of model rats, regulate and control the heart functions of the model rats, improve the systolic function of the heart, relieve expression of inflammatory factors such as interleukin-1beta, interleukin-6 and tumor necrosis factor-alpha of the rats and improve the anti-inflammatory effect of the rats. The action mechanism of the compound relates to a plurality of pathways such as extracellular matrix receptor interaction, a chemotactic factor signal pathway, a relaxin signal pathway, a cyclic adenosine monophosphate signal pathway, a nuclear factor-kB signal pathway, fatty acid metabolism and a Toll-like receptor signal pathway, and the compound is an effective medicament for treating ischemic heart failure.

The application of a drug or medical aesthetic product containing tolvaptan as an active ingredient for inhibiting melanin synthesis. Cell activity detection and melanin content determination show that tolvaptan can significantly reduce the intracellular melanin content without affecting the cell activity. Tyrosinase activity detection shows that tolvaptan does not affect the catalytic activity of TYR. Western blotting experiment shows that tolvaptan can down-regulate the expression levels of TYR family proteins, microphthalmia-associated transcription factor (MITF), cyclic adenosine monophosphate response element binding protein (CREB) and melanocortin 1 receptor (MC1R).

The application discloses a kind of serum-free epidermal melanocyte culture medium and its application, method includes by dissolving adenine, hepatocyte growth factor (HGF), glutamine, retinoic acid, vitamin E, nicotinic acid, sodium selenite, sodium hyaluronate and recombination human type XVII collagen are configured into initial culture medium;Preparation of melanocyte stimulating hormone, endothelin-3, transferrin, triiodothyronine, cholesterol, neuroregulin 1 (NRG1), 3-isobutyl-1-methyl purine (IBMX), dibutyryl cyclic adenosine monophosphate (db-cAMP), cholera toxin, hydrocortisone and recombination human insulin mother liquor;Step three: according to culture and concentration, a certain amount of mother liquor of step two is added to initial culture medium.The culture medium can effectively promote human epidermal melanocyte proliferation, melanin accumulation and help to promote human umbilical cord mesenchymal stem cells into human epidermal melanocyte, provide key advantage for obtaining functional melanocyte with high activity and therapeutic potential.

The application relates to the field of biological technology, in particular to application of cyclic adenosine monophosphate in promoting biomass increase of pasture and root nodule, wherein the cyclic adenosine monophosphate significantly improves the fresh weight biomass of the above-ground part, the root part and the single-plant root system root nodule of Medicago truncatula inoculated with rhizobium, and the difference is significant.

This invention discloses a method for preparing a molecularly imprinted electrochemical sensor for detecting cyclic adenosine monophosphate (cAMP). The sensor uses reduced graphene oxide (RGO) and gold nanoparticles as modifying materials. RGO is drop-coated onto the surface of a glassy carbon electrode and dried to obtain a modified electrode RGO / GCE. The RGO / GCE is then placed in a sulfuric acid deposition solution containing chloroauric acid, and gold nanoparticles are used to modify the RGO / GCE surface using a potentiostatic method to form a gold substrate, resulting in a deposited electrode AuNPs / RGO / GCE. The AuNPs / RGO / GCE is then electropolymerized in an electrolyte polymerization solution containing the template substance cAMP and the functional monomer o-phenylenediamine to obtain a dense and insulating polymer film. The polymerized electrode is immersed in an elution solution and eluted using cyclic voltammetry to obtain MIP / AuNPs / RGO / GCE. This sensor exhibits specific recognition selectivity for cAMP, high detection sensitivity, low detection limit, good reusability, and stability.

The present disclosure is directed to methods (e.g., in vitro methods) for detecting the presence of neutralizing antibodies to PTH or PTHrP analog in a sample. The in vitro method comprises the steps of obtaining a sample from a subject; contacting the sample with a cell; measuring cyclic adenosine monophosphate (cAMP) levels; and detecting the presence of neutralizing antibodies, when cAMP levels are reduced relative to a negative control sample without neutralizing antibodies. An in vitro method of detecting the presence of neutralizing antibodies in a sample from a subject treated with Abaloparatide is also provided. Further provided herein is a kit for carrying out the methods described herein comprising components required to carry out the obtaining, contacting, measuring and detecting steps and instructions for use.

The present application relates to the technical field of diagnosis markers and diagnosis methods for mental illness of children and adolescents, in particular to a plasma metabolic diagnosis marker and a diagnosis system for depression of children and adolescents. The plasma metabolic diagnosis marker comprises at least one of cyclic adenosine monophosphate, methylpyridine, unsaturated undecylenoyl carnitine, histidinol, prolyl hydroxyproline, tryptophan and glucuronic acid. The present application analyzes non-target metabolomics data, and determines seven candidate diagnosis markers for depression of children and adolescents from common differential metabolites. Then, through targeted metabolomics research, diagnosis markers and their combinations with ideal diagnosis effects are determined, and a diagnosis model is constructed. The gender factor is also included in the diagnosis model to further improve the diagnosis effect. The present application can solve the technical problem that the reliability and promotion potential of the plasma metabolic biomarkers for depression of children and adolescents in the prior art are not ideal, and has an ideal promotion and application prospect.

The present application relates to a kind of traditional Chinese medicine composition for treating ischemic heart failure and preparation method thereof, belong to the field of traditional Chinese medicine pharmacy, the traditional Chinese medicine composition includes: cassia 5-15 parts, white atractylodes 5-15 parts, poria 5-15 parts, dried ginger 6-12 parts, acanthopanax 5-15 parts, water red flower seed 10-30 parts, verbena 3-9 parts, dogwood 6-12 parts, fried licorice 3-9 parts.The composition can significantly reduce N-terminal B-type natriuretic peptide level, significantly improve model rat heart tissue pathological changes, regulate the heart function of model rat, improve the systolic function of heart, and can reduce the expression of rat interleukin-1 beta, interleukin-6, tumor necrosis factor-alpha and other inflammatory factors, involve extracellular matrix receptor interaction, chemotactic factor signal pathway, relaxin signal pathway, cyclic adenosine monophosphate signal pathway, nuclear factor-kappa B signal pathway and multiple pathways, and is effective drug for treating ischemic heart failure.

This invention discloses a type of Lactobacillus paracasei ( Lactobacillus paracasei Preparation of Lactobacillus paracasei F5 and polysaccharide synbiotic composition and its application in alleviating behavioral cognitive impairment in the elderly. This strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 35145, dated July 9, 2025. The synbiotic composition consists of lyophilized Lactobacillus paracasei F5 powder and arabinoxylan (AX) or galactooligosaccharides (GOS) in a specific mass ratio, with a viable count ≥ 1 × 10⁻⁶. 8 CFU / g. In an Aβ transgenic fruit fly model, administration of the synbiotic for 8 consecutive days significantly improved the climbing ability of the model fruit flies and reduced Aβ levels in the brain. 42 Levels of AChE and cyclic adenosine monophosphate (cAMP) were observed. In a mouse model induced by AlCl3 combined with D-gal, continuous gavage for 11 weeks significantly reversed learning and memory deficits and downregulated Aβ levels in the hippocampus. 42 This study aims to reduce the deposition and AChE activity of pro-inflammatory factors in the cerebral cortex, providing a safe and feasible strategy for dietary intervention in cognitive impairment in the elderly, with broad application prospects.