73 results about "Testosterone" patented technology

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

The invention belongs to the technical field of medical experimental animal models, and particularly relates to a construction method of a rabbit ear acne model for studying acne pathogenesis and drug curative effect. A healthy New Zealand large-ear white rabbit is selected, a testosterone solution is subcutaneously injected to induce endocrine disorder, oleic acid is smeared at an opening of an external auditory canal on the inner side of an ear, dermatobacterium acnes suspension is subcutaneously injected, and whether acne-like lesions such as erythema, papule and comedo appear on the skin of the ear or not is observed. The method is easy and convenient to operate, low in cost, good in repeatability, capable of simulating the pathological process of human acne and suitable for acne pathogenesis research and drug screening.

The invention discloses a non-specific peroxidase HecUPO derived from Hypoxylon sp. (strain EC38), wherein the amino acid sequence of the non-specific peroxidase HecUPO is as shown in SEQ ID NO: 1. The enzyme can efficiently catalyze testosterone to generate 16 alpha-hydroxylation reaction to generate C16 alpha-hydroxytestosterone, the conversion rate reaches 70%, the separation yield reaches 82%, no by-product is generated, and the C16 alpha-hydroxytestosterone has excellent regioselectivity. The invention also discloses a gene for coding the HecUPO, a recombinant plasmid pPICZA-HecUPO containing the gene, a recombinant pichia pastoris engineering bacterium and an application of the recombinant pichia pastoris engineering bacterium in steroid hydroxylation. The non-specific peroxidase HecUPO disclosed by the invention has the advantages of being high in catalytic efficiency, specific in regioselectivity, simple in reaction system, green, environmentally friendly and the like, and a new enzymology resource is provided for fixed-point hydroxylation modification of steroid drugs.

A trans-mucosal testosterone delivery composition for humans or animals having from about 50 to 99 wt. % humectant, from about 1 to 50 wt. % water, from about 0.001 to 25.00 wt. % testosterone, from about 0 to 25 wt. % oil, and from about 25 to 1 wt. % surfactant, wherein the composition is hydrophobic and has release properties.

The invention belongs to the technical field of biological medicines, and provides a method for synthesizing testosterone 17-O-beta-D-glucosyl-(1-> 4)-6 ''-O-acetyl-beta-D-glucoside through two-step enzymatic coupling. Mainly relates to an intermediate compound testosterone 17-O-beta-D-glucosyl-(1-> 4)-beta-D-glucoside for synthesizing the molecule, mutant amino acid and nucleotide sequences of glycosyl transferase OsSGT2 for synthesizing the intermediate, and synthesis application. In addition, the invention also provides an application of the testosterone 17-O-beta-D-glucosyl-(1-> 4)-6 ''-O-acetyl-beta-D-glucoside in liver protection.

The invention discloses a method for detecting related substances in testosterone enanthate by high performance liquid chromatography. A mobile phase A and a mobile phase B are used as mixed mobile phases for gradient elution; the mobile phase A is water; the mobile phase B is acetonitrile; the volume ratio of the mobile phase A to the mobile phase B is (5%-70%): (30%-95%); a chromatographic column adopted by the high performance liquid chromatography method is Agilent ZORBAX Eclipse XDB-C8, and the detection method is characterized in that the detection method is simple and easy to implement. According to the method, the testosterone enanthate and the related substances thereof are separated and detected by screening a stationary phase chromatographic column and optimizing a mobile phase elution mode and an elution component ratio, and compared with a method in European Pharmacopoeia, after the chromatographic column is subjected to gradient elution, not only is the retention time of an impurity D prolonged, but also the quality of the impurity D is improved. And the separation degree of the impurity F and the impurity G is increased, so that the elution capacity and the separation degree among the impurities are enhanced.

The present application belongs to the field of nutritional biotechnology, in particular, the present application relates to an anti-aging composition and its application. Through a large number of research and screening, it is found that the combination of specific grape seed extract, ergothioneine and specific black ginger extract can exert the synergistic effect of the three, fully exert the grape seed extract (antioxidant, free radical scavenging), black ginger (testosterone enhancement, muscle function enhancement), ergothioneine (protection of mitochondria and lysosome), form a multi-pathway mechanism, multi-level improvement of aging, improvement of fertility and muscle capacity.

This invention provides a method for simultaneously detecting free and total testosterone in serum. Free testosterone is released from a serum sample using equilibrium dialysis, followed by derivatization. An additive quencher is added to remove excess derivatizing reagent, and then a sample solution containing total testosterone is added for testing. The method provided by this invention is simple to operate, eliminating the need for complex procedures such as nitrogen blowing and liquid-liquid extraction. The quencher chosen is low-toxicity, low-cost pyruvate, which improves detection sensitivity while reducing experimental costs. Furthermore, this invention utilizes the biuret assay to determine whether protein leakage has occurred during equilibrium dialysis, providing a direct and accurate assessment.

A method and oral composition for treating a plurality of end-stage diseases, such as end-stage renal disease, chronic kidney disease, liver cirrhosis, chronic liver failure, end-stage liver disease, cancer, congestive heart failure, pulmonary fibrosis, and chronic obstructive pulmonary disease, are disclosed. In one embodiment of the invention, the composition comprises testosterone duodecanoate.

The present disclosure relates to a specific combination of a progestin and testosterone in a transdermal composition and usage thereof in a method for ensuring male contraception with more efficacy and less side effects.

The invention provides a traditional Chinese medicine composition for preventing hair loss and promoting hair growth and application thereof, and relates to the technical field of hair loss prevention and promoting hair growth, the traditional Chinese medicine composition is composed of Chinese doughnut, cockscomb, albizia flower, Chinese honey locust, schizonepeta, lithospermum, polygonum multiflorum, prepared rehmannia root, notopterygium root, radix angelicae pubescentis, ruddle and periostracum cicada; in-vitro experiments show that the traditional Chinese medicine composition can promote proliferation of human dermal papilla cells HDPC, relieve the damage degree of HDPC and induce cycle transformation of hair follicles, so that the effect of promoting hair growth is achieved; animal experiments show that the traditional Chinese medicine composition can significantly increase the number of hair follicles and the thickness of skin and dermis fat of mice, reduce the levels of mouse serum IL-2, TNF-alpha and TGF-beta1 and reduce the levels of testosterone, dihydrotestosterone and estradiol in mouse skin tissue, so that hair follicle fibrosis is inhibited, the growth period of hair follicles is accelerated, the growth period is prolonged, the hair loss phenomenon is improved, and the hair loss rate is increased. The hair growth is promoted.

The present invention relates to a pharmaceutical composition comprising a steroid such as testosterone or a functional analogue or derivative thereof, in combination with a PDE5 inhibitor such as sildenafil. The testosterone or the functional analogue or derivative thereof is present in an amount equivalent to no less than 1 mg. The present invention also concerns the use of the pharmaceutical composition for enhancing female sexual desire in the treatment of FSIAD of a patient, as well as methods for identifying suitable patients and for identifying suitable dosage for the use thereof.

The application discloses an oil control composition containing tea extract and products and applications thereof. The oil control composition containing tea extract comprises a tea extract solution, a vitagenin solution and an aesculetin solution. Researches show that the oil control composition has a good oil control effect, can reduce the intracellular IDH concentration by inhibiting the expression level of IDH, thereby regulating the content of testosterone, and achieving the effect of regulating the secretion of skin oil. Meanwhile, the composition is further prepared into oil control cosmetics and used on the skin, can effectively improve the oil absorption and decontamination capacity, can reduce the adhesion and fluidity with the skin, improve the comfort of the user, has a fresh skin feeling, can remove the overflowed oil on the skin, and can control the oil for a long time, moisturize, and effectively improve the state of oily skin and dry skin. In addition, the raw materials used in the application are easy to obtain, can be extracted from plants, are non-toxic and harmless, and are safer to use.

The invention belongs to the field of basic medical research, and particularly relates to a testicular toxicity animal model establishing method which comprises the following steps: performing intragastric administration on male rats with a preparation containing podophyllotoxin according to the dosage of 15 mg / kg per day; the medicine is continuously administrated for 4 days; according to the method, the rat can be induced to generate testicular toxicity phenotypes, and the phenotypes at least include that the number of sperms is remarkably reduced, the activity of the sperms is reduced, the testosterone level of serum is abnormally increased, and spermatogenic tubules in testicular tissue are irregular in shape and spermatogenic epithelium is thinned.

Provided are previously unknown novel uses of 5-deazaflavin derivatives and related compounds.　Provided are: an antioxidative stress agent containing, as an active ingredient, a 5-deazaflavin compound represented by general formula (I), a pyridodipyrimidine compound represented by general formula (II), a deazaflavinotestosterone compound represented by general formula (III), or a deazaflavinocholesterol compound represented by general formula (IV); and a cosmetic, a quasi-drug, and a pharmaceutical each containing any of the compounds.

The invention discloses a non-alcoholic fatty liver biomarker composition and application thereof, and belongs to the technical field of non-alcoholic fatty liver detection. The non-alcoholic fatty liver biomarker composition comprises at least two of citric acid, linoleic acid, L-glutamic acid, L-glycine, asparaginic acid, progesterone, testosterone and uric acid, and further, the hepatic fibrosis biomarker further comprises at least one of 2-hydroxyestradiol, L-citrulline, L-procholecin, N-acetyl-L-methionine and flavin. The biomarker composition is stable, the possibility of misdiagnosis and missed diagnosis of diseases is greatly reduced, and compared with a single omics analysis method, the biomarker composition is more suitable for the occurrence and development mechanism of the diseases.

The invention discloses a nutrient composition for improving the male fertility function and a preparation method and application of the nutrient composition. The composition is composed of zinc, selenium, coenzyme Q10, L-acetyl carnitine, folic acid, vitamin D3, natural lycopene, vitamin E, L-arginine and core components such as standardized herba epimedii, fructus lycii and maca extract according to a specific ratio, and three pairs of key mass ratios of zinc / selenium, L-acetyl carnitine / coenzyme Q10 and natural lycopene / vitamin E are limited for the first time. The composition can synergistically improve sperm quality, reduce oxidative stress and improve serum testosterone level in a multi-target manner, the invention further provides a preparation method of the composition, the uniformity and stability of the composition are ensured through the processes of graded mixing, low-temperature sterilization and the like, and the composition can be used for preparing dietary supplements or health care products for improving oligospermia, asthenozoospermia and abnormal spermatozoospermia.

A method of determining an amount of testosterone in each of a plurality of human samples with a single mass spectrometry includes subjecting each of the plurality of human samples to a different derivatizing agent to produce a different derivatized testosterone in each of the plurality of samples; combining the plurality of samples to form multiple samples; and quantifying the amount of testosterone in each sample by mass spectrometry.

The invention relates to a composition and a preparation method of a reproduction nursing external preparation with effects of inhibiting bacteria, relieving and improving male functions. The invention discloses an external preparation for male reproduction nursing. The plant essential oil comprises natural plant extracts (clove, herba epimedii, ginseng, dendrobium officinale, tribulus terrestris, radix sophorae flavescentis, fructus cnidii, saw palmetto fruit and aloe vera), nutritional ingredients (arginine, hydrolyzed collagen zinc, nicotinic acid, tocopherol, biotin and B-group vitamin yeast polypeptide) and compound essential oil (tea tree oil, jasmine flower oil, iris oil, cananga odorata flower oil and sandalwood oil). The composition can be prepared into spray, cream, lotion and other dosage forms. The preparation improves erectile function, enhances microcirculation, relieves pressure, improves sexual desire and realizes precise adjustment of male function and protection of gonad axis through mechanisms of inhibiting bacteria, diminishing inflammation, improving testosterone concentration, enhancing nitric oxide synthase activity, promoting vasodilatation, blocking neural signals, delaying ejaculation and the like.

The invention relates to a compound microbial agent and application thereof, belongs to the technical field of microorganisms, and particularly discloses application of mixed fermented milk in a product for improving male functions. According to the application, the new application of the mixed fermented milk of the rhamnosus lactobacillus B6 and the streptococcus thermophilus XJ53 (CGMCC No.17323) in the aspect of improving male functions is disclosed for the first time, and the new application is specifically characterized by improving male fertility desire, reproductive capacity, seminiferous tubule dysfunction and spermatogenesis, testis oxidative stress activity, testis testosterone synthesis and sperm quality; the strain has a potential value of producing a gain substance for improving the male function by fermenting skimmed milk, and also prompts that the fermented milk has a potential application prospect for enhancing the male function.

The present disclosure relates to a specific combination of a progestin and testosterone in a transdermal composition and usage thereof in a method for ensuring male contraception with more efficacy and less side effects.

The invention relates to the technical field of tumor immunotherapy, in particular to an RPL39L gene and application of a gene inhibitor of the RPL39L gene, and the RPL39L gene comprises an RPL39L gene knockout agent and small interfering RNA (Ribonucleic Acid) targeting the RPL39L gene. According to the invention, the cancer testosterone gene RPL39L is found to be ribosomeST for forming spermatogenic cell specific ribosomeST for the first time, and can regulate and control a plurality of downstream cancer testosterone genes. A gene inhibitor and small interfering RNA are designed for the RPL39L, the RPL39L is knocked out from various tumor cells such as osteosarcoma, colorectal cancer, leukemia and glioma by using a CRISPR / Cas9 technology, and the influence on the proliferation ability and migration ability of the tumor cells after the RPL39L is knocked out is proved in vitro; and shRNA of a target RPL39L coding region is designed, so that the expression level of the RPL39L in tumor cells is successfully inhibited, and the inhibition effect of the shRNA on the growth of the tumor cells is proved in vitro. The invention provides an effective means for inhibiting the expression of the cancer testosterone gene regulatory factor from the upstream, and has extremely important significance and application prospect in tumor treatment.

The invention belongs to the technical field of microorganisms, and particularly discloses application of lactobacillus rhamnosus fermented milk in a product for improving male functions. According to the application, the new application of the lactobacillus rhamnosus B6 (CGMCC No.13310) fermented milk in the aspect of improving male functions is disclosed for the first time, and the new application is specifically characterized by improving male fertility desire, reproductive capacity, seminiferous tubule dysfunction and spermatogenesis, testis oxidative stress activity, testis testosterone synthesis and sperm quality; the strain has a potential value of producing a gain substance for improving the male function by fermenting skimmed milk, and also prompts that the fermented milk has a potential application prospect for enhancing the male function.

Disclosed is a method and oral composition for treating a plurality of End stage diseases such as end-stage renal disease, chronic kidney disease, liver cirrhosis, chronic liver failure, end stage liver disease, Cancer, congestive Heart failure, pulmonary fibrosis, chronic obstructive pulmonary disease. In an embodiment of the invention, the composition comprises testosterone duodecanoate.

Probes that are versatile, easy to use, and provide rapid results for detecting and quantifying levels of small molecules that include steroids, hormones, antibodies, aptamers and enzymes such as various steroidal hormones like estrogen, progesterone and testosterone in samples. This is particularly useful in home and clinical settings. A probe useful in competitive assays includes a competitive ligand bound to a linker molecule bound to a detectable tag. The linker may be chemical, DNA or a combination of both.

The application provides a kind of sandwich detection testosterone monoclonal antibody and its preparation method and application, comprising: step S1, preparation testosterone-3-(O-carboxymethyl) oxime, and it is activated;After activation, take testosterone antibody P2G1, according to proportion, drop the activated testosterone-3-(O-carboxymethyl) oxime is reacted to prepare immunogen;Step S2, based on the prepared immunogen, immunization animal;Step S3, the spleen cell of immunization animal and myeloma cell are fused into hybridoma cell, and the real hybridoma cell is selected;Step S4, antibody is separated and purified from the screened hybridoma cell.The application provides a kind of preparation method of raw material and reagent for testosterone sandwich method detection, solves the problem that the sensitivity of small molecule compound testosterone competition method reagent is insufficient, detection range is narrow, specificity is poor, provides a new thought and method for the rapid, accurate detection of testosterone.

The invention provides a method for simultaneously detecting free testosterone and total testosterone in serum, which comprises the following steps of: releasing the free testosterone from a serum sample by adopting an equilibrium dialysis method, deriving the free testosterone, adding a quenching agent to remove excessive derivation reagent after derivation, and then adding a sample solution containing the total testosterone to test. The method provided by the invention is simple and convenient to operate, complex operations such as nitrogen blowing and liquid-liquid extraction are not needed, pyruvic acid with low toxicity and low cost is selected as a quenching agent, and the experiment cost is reduced while the detection sensitivity is improved. In addition, whether the protein leaks or not in the equilibrium dialysis process is judged by utilizing a biuret experiment, and the method is visual and accurate.

The application discloses a method for simultaneously determining the content of total testosterone and free testosterone in blood, which comprises the following steps: step one, dividing a blood sample into two sample parts A and B, and obtaining a sample total testosterone solution by processing the sample A; step two, obtaining a sample free testosterone solution by performing equilibrium dialysis and derivative reaction on the sample B; step three, reconstituting the sample total testosterone solution and the sample free testosterone solution with a reconstitution solution and mixing to obtain a sample total solution; and step four, simultaneously detecting the content of total testosterone and free testosterone in the sample total solution by using LC-MS / MS technology, and obtaining the content of total testosterone and free testosterone in the sample total solution. The application has the beneficial effect of simultaneously determining the content of total testosterone and free testosterone. The application provides a kit for simultaneously determining total testosterone and free testosterone, which can rapidly detect total testosterone and trace free testosterone, and the detection limit of the free testosterone can reach 0.5 pg / mL.

The present invention relates to a pharmaceutical composition for preventing or treating muscle diseases. Specifically, the elderberry extract or monosaccharide and amino acid combined compound disclosed by the invention inhibits apoptosis of muscle cells, inhibits expression of inflammatory cytokines, promotes secretion of testosterone and inhibits expression of amyotrophy marker protein and myogenesis inhibition protein, and shows an effect of recovering muscle in a sarcopenia animal model; therefore, the device can be effectively used in the treatment of muscle diseases.