137 results about "Laminin" patented technology

The invention relates to the field of biological medicine and cosmetics, in particular to laminin RL-20 and application of the laminin RL-20 in cosmetics.

The invention discloses a composition with sensitive skin repairing and anti-aging functions as well as an emulsion and application of the composition, and belongs to the technical field of cosmetics. The composition specifically comprises a pseudoalteromonas fermentation product extract, collagen, palmitoyl tripeptide-5, acetylchitosamine, sodium hyaluronate, silanetriol, caffeine and acetyl hexapeptide-8, and can promote the generation of laminin-5 and integrin beta1, promote the vitality of keratinocytes and fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, and promote the growth of the keratinocytes and the fibroblasts. The generation of inflammatory factors (IL-6) is inhibited; the generation of collagen IV and collagen VII is promoted; the invention further provides an emulsion prepared from the composition, and the optimal formula of the emulsion is obtained through optimization. The active composition disclosed by the invention has a wide application prospect in the field of cosmetics, and particularly has important significance in sensitive skin repair and anti-aging directions.

The invention discloses a skin care product anti-aging effect multi-dimensional comprehensive detection system, which comprises an in-vitro cell model experiment module for detecting indexes such as cell activity, proliferation, migration, apoptosis and antioxidant enzyme activity by simulating natural aging and light aging conditions; the content change of skin structure components such as collagen, elastic fibers and laminin is quantitatively analyzed, the DNA damage repair capacity is reflected by measuring the expression quantity of gammaH2AX, epigenetics research is developed, and the methylation state change of a CpG island is monitored; and the tissue engineering and three-dimensional skin model experiment module is used for performing morphological observation, biochemical index detection and skin physiological parameter evaluation under the condition of simulating a real skin environment by utilizing an artificial skin model. The whole process from the in-vitro cell model to the clinical human body test is covered, and the actual efficiency of the skin care product for delaying or improving the skin aging process can be accurately and objectively evaluated through multi-angle and multi-level data integration analysis.

Disclosed herein are pharmaceutical agents and compositions that activate integrin heterodimers that contain beta-1 integrin protein that specifically bind laminin protein. Such agents and compositions are useful for treating diseases related to muscle malfunction, including muscular dystrophies.

The invention relates to the technical field of organoid culture, in particular to tumor organoid culture hydrogel with definite components and a preparation method and application thereof, and the tumor organoid culture hydrogel with the definite components comprises a gel precursor and laminin 521; the gel precursor is prepared by dissolving sodium alginate and gelatin in PBS; the concentration of the laminin 521 is 0.10 mg / ml to 0.15 mg / ml. The hydrogel provided by the invention is clear in component, the performance of the hydrogel for culturing the tumor organoid is equivalent to that of Matrigel, and the technical problems that Matrigel is complex in component, different in batches and high in price, and consequently conversion and application of a tumor patient source organoid model are limited are solved. Besides, the hydrogel provided by the invention is high in component industrialization degree, good in stability and easy to obtain, standardized and economic production can be realized, and support is provided for construction of a tumor organ model.

The invention discloses a model for predicting the progression hepatic fibrosis risk of a WD long-term maintenance treatment patient and a design method thereof, and relates to the technical field of medical information, the prediction model comprises the following steps: an input module receives patient parameter indexes composed of sex (SEX), platelet (PLT), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (Apo-A1), laminin (LN) and copper cyanin (CER), and the input module receives the patient parameter indexes composed of sex (SEX), platelet (PLT), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (Apo-A1), laminin (LN) and copper cyanin (CER); the processing module obtains single scores corresponding to the seven independent risk factors, the single scores are added to obtain a total score, the prediction probability corresponding to the total score is the risk of the progression-stage hepatic fibrosis of the WD long-term maintenance treatment patient, and the prediction risk probability is output through the output module. The column diagram prediction model established by the invention has relatively high accuracy, and can be conveniently used for early recognition and risk prediction of progression-stage hepatic fibrosis of WD long-term maintenance treatment patients.

The invention provides a method for culturing primary spermatogonial stem cells of Tibetan sheep, and belongs to the technical field of cell separation and culture. The culture method provided by the invention comprises the following steps: disinfecting and cleaning testis tissues of Tibetan sheep lambs; shearing tissue blocks, and digesting and cleaning to obtain a single-cell suspension of the seminiferous tubule; purifying the single-cell suspension, and culturing without a feeding layer or with the feeding layer; the culture without the feeding layer refers to culture in a laminin-coated culture container by using a feeding-layer-free MEM alpha culture medium or a StemProTM-34SFM complete culture medium; culture with a feeding layer refers to culture in a mouse fibroblast line feeding layer or a Tibetan sheep primary testicular sertoli feeding layer, and a feeding layer MEMS alpha culture medium is added. According to the culture method provided by the invention, the Tibetan sheep spermatogonial stem cells with higher purity can be obtained through separation, the subsequent proliferation of the spermatogonial stem cells can be remarkably promoted, and the apoptosis of the spermatogonial stem cells can be inhibited.

The invention discloses recombinant laminin with anti-aging and repairing effects and a preparation method of the recombinant laminin, relates to the technical field of recombinant proteins, and aims to solve the technical problems that existing laminin is relatively low in yield and limited in application. According to the recombinant laminin with the anti-aging and repairing effects, the sequence of the recombinant laminin is shown as SEQ ID No.3, the total number is 363aa, and the predicted molecular weight is 40.4 kD. Compared with the prior art, the recombinant laminin is synthesized by embedding a laminin gene sequence into a pichia pastoris genome for heterologous expression. A cytological detection method for evaluating the efficacy of a skin care product is applied, and a research result shows that the recombinant laminin can remarkably promote cell adhesion and migration activity and can stimulate cells to generate collagen, so that the anti-aging and repairing functions of the recombinant laminin are shown.

The present invention relates to modified recombinant agrin proteins or fragments thereof and recombinant chimeric laminin-nidogen proteins or fragments thereof, nucleic acid sequences encoding these proteins, vectors containing these nucleic acid sequences, compositions comprising these proteins, and the use of these proteins in the prevention and / or treatment of congenital muscular dystrophies, in particular (laminin-α2) LAMA2-associated muscular dystrophy.

The invention provides a method for repairing a wound surface by activating fibroblasts. Specifically, the invention provides a nanofiber mat, the nanofiber mat continuously delivers curcumin to activate fibroblasts, and the activated fibroblasts further utilize an EMMPRIN and CCL2 target mediated pathway to complete the repair of a steady-state basement membrane. Experimental results show that the delivery mode can effectively activate fibroblasts, and the activated fibroblasts regenerate skin by inhibiting expression of EMMPRIN and CCL2 targets and promoting expression of laminin, so that acute wounds and chronic wounds are treated. The invention also provides a method for preparing the nanofiber mat.

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located on top of and within the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high levels of laminin-5, a cell adhesion bio-active factor shown to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.

The invention relates to a hydrogel material containing laminin, the hydrogel material comprises a hydrogel body and an effective amount of laminin subunit arranged in the hydrogel body, and the laminin subunit is cross-linked with the hydrogel body. The laminin subunit can be added into the hydrogel, so that the polarity of the dental papilla cell of the miniature pig and the differentiation level of the dentin-forming cell are remarkably improved, the differentiation of the dentin-forming cell is directionally regulated and controlled, the differentiation level of the dentin-forming cell is effectively improved, the operation is simple, and the effect is remarkable. In addition, laminin subunits are added into the collagen-based hydrogel, so that the rigidity of the collagen hydrogel is improved, and the arrangement and mechanical strength of collagen fibers in the hydrogel are improved.

The invention belongs to the technical field of biological medicines, and particularly relates to application of MIM2 and derivatives thereof in preparation of products for treating tissue fibrosis. It is found that the compound MIM2 can effectively inhibit expression and synthesis of various cancers (extracellular matrix proteins such as collagen and laminin in cells). In-vitro experiments show that after MIM2 treatment, collagen and laminin expression of the cancer cells, skin fibroblasts, hepatic stellate cells and embryonic lung cells is remarkably reduced. Animal experiments further prove that the MIM2 can effectively inhibit collagen deposition in tumor tissues of tumor-bearing mice, can slow down the fibrosis process of liver fibrosis and pulmonary fibrosis mouse models, and can reduce the level of plasma hyaluronic acid at the same time. The results show that the MIM2 has the effects of inhibiting extracellular matrix deposition and delaying tissue fibrosis, and is expected to be developed into small molecular drugs for treating cancer-related fibrosis and other fibrosis diseases.

A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes and method of manufacture. A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes, comprising: a first layer of ADM of biological origin arranged inferiorly to delimit a cavity with a lower edge; a filling material disposed in the cavity surrounded by the first layer of ADM consisting of a ground ADM having the consistency of a fibrous paste; and a second layer of ADM of biological origin arranged on the upper part covering the filling material and whose upper edge rests peripherally on the lower edge of the first layer of ADM, thus defining a rim;where the first layer of ADM and the second layer of ADM are joined by an eyebrow overlap along the upper edge of the first layer of ADM with the lower edge of the peripheral rim using a biocompatible adhesive, or by absorbable suture; where the first layer of ADM, the second layer of ADM and the ADM filling material are not immunogenic; and where the ADM comprises collagen fibers, fibronectin, elastin, laminin, glycosaminoglycans and hyaluronic acid. Method for its manufacture.

The first problem of the present invention is to provide a novel technique with which it is possible to produce iPS cells using epithelial cells such as keratinocytes. The present invention for solving the problem is a method for producing an artificial pluripotent stem cell using an epithelial cell, the method comprising: an initialization step for introducing a reprogramming gene or a translation product thereof, which has been subjected to nucleus initialization, into the epithelial cell; and an adhesion culture step in which the cells that have undergone the initialization step are subjected to adhesion culture on a culture surface coated with laminin or a fragment thereof.

The invention discloses a biomarker for monitoring the curative effect of a PD-1 antibody of a PD-L1Positive gastric cancer patient, and relates to the technical field of biomedical detection, and the biomarker is laminin gamma 2. According to the invention, the laminin gamma2 is used as a novel biomarker for monitoring the curative effect of the PD-1 treatment antibody of the PD-L1Positive gastric cancer patient, experiments prove that the serum level of the laminin gamma2 is higher than that of healthy people in the gastric cancer patient and is in positive correlation with a prognosis related factor sPD-L1, the tissue expression of the PD-L1Positive patient is higher, and the serum level of a person without PD-1 antibody treatment response is higher; it is proved that the marker has good progress monitoring value, treatment reaction differences can be effectively distinguished, and the blank of lack of PD-1 antibody curative effect monitoring markers for patients of the type is filled up.

The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.

To provide methods for inducing differentiation to ectodermal cells excellent in differentiation-inducing efficiency from pluripotent stem cells to ectodermal cells and excellent in culture operability as well as methods for making such cells.SOLUTION: Methods for inducing differentiation from pluripotent stem cells to ectodermal cells comprise the steps of: (1) coating a cell culture substrate with one or more substances selected from the group consisting of Matrigel, laminin, fibronectin, vitronectin and collagen where the substrate comprises a region having cell adhesiveness and cell-proliferating property and a region having neither of them and then seeding pluripotent stem cell 1; (2) conducting adhesion culture of the seeded pluripotent cells in the presence of an undifferentiation-maintaining medium in feeder-free conditions to form agglutination of pluripotent stem cells 2 adhered to the cell culture substrate; (3) culturing the formed agglutination of pluripotent stem cells in the presence of a culture medium containing a differentiation-inducing agent so that the cells adhered to the cell culture substrate form germ layer 3; and (4) culturing the formed germ layer in the presence of a culture medium containing the differentiation-inducing agent in the adhered state to the cell culture substrate to form ectodermal cell aggregates 4.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of animal reproductive biology and cell engineering, and particularly relates to a fluorescence labeling expression system for visualizing an oocyte nucleus maturation process and application of the fluorescence labeling expression system. The fluorescence labeling expression system comprises an eukaryotic expression vector pVenus-LMNB1; the eukaryotic expression vector pVenus-LMNB1 comprises a nucleotide sequence for coding the fusion protein Venus-LMNB1. According to the invention, a nuclear fiber layer protein stably expressed on a nuclear membrane is selected as a research object, a eukaryotic expression vector pVenus-LMNB1 is constructed by using a molecular cloning technology, an LMNB1 gene containing a green fluorescent label Venus is overexpressed in a porcine oocyte by using a microinjection technology, fluorescent protein labeling of a structural protein on the nuclear membrane is utilized, and the expression vector pVenus-LMNB1 is constructed. The expression positioning condition of the LMNB1 in the maturation process of the porcine oocytes is observed, and a powerful tool is provided for development of in-vitro culture related researches of the porcine oocytes.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The present disclosure describes methods of maintaining the phenotype of differentiated cells. Generally, the natural environment of the body is replicated for the differentiated cell. The differentiated cell is plated on a cell culture substrate comprising a laminin, such as laminin-521 or laminin-511. The substrate may also contain a cadherin. This maintains the phenotype of the differentiated cell.

The present invention provides a method for promoting the differentiation of mesenchymal stem cells (MSCs) into oligodendrocyte progenitor cells, which includes primary culturing the isolated MSCs, followed by secondary passage culturing, then treating them with a gene expression vector containing the "key gene for oligodendrocyte progenitor cell differentiation", and further performing differentiation culture using the "oligodendrocyte progenitor cell differentiation medium". Through specific cell differentiation treatment and culture processes, the present invention uses a cell culture matrix treated with recombinant human laminin to culture MSCs and promote their differentiation, so as to obtain oligodendrocyte progenitor cells derived from MSCs in a relatively short time. The present invention further provides a method for efficiently harvesting oligodendrocyte progenitor cells. Specifically, through a rate-limiting shaking method, oligodendrocyte progenitor cells are harvested and allowed to further proliferate, and a relatively large amount of oligodendrocyte progenitor cells with high purity can be harvested in a short time for the production of cell therapy products.

The method comprises providing a culture of human pluripotent stem cells affixed to a first matrix comprising a first laminin; exposing the stem cells to a differential medium for a first period of time from 15 days to 50 days to obtain a first adherent cell population comprising RPE cells and / or ancestors thereof; dissociating the first population from the first matrix at the end of the first time period; re-planking the dissociated first cell population on a second substrate comprising a second laminin; and culturing the re-planked first cell population on a second substrate for a second period of time to obtain an expanded and mature second cell population comprising RPE cells. The first and second laminins may be independently selected from the group consisting of LN-521, LN-511, LN-111, and LN-121, and are intact proteins or protein fragments. In some embodiments, the cell surface marker, which can be used to generate RPE cells in vitro, is selected from the group consisting of CD140b, CD56, CD104, CD164, CD220, EGFR, GD2, CD184, CD10, CD30, CD49a, CD49b, CD50, CD171, TRA-1-60, and CD326, preferably CD140b, CD56, GD2, and / or CD184. Also included are RPE cells so produced, as well as materials and compositions that utilize such RPE cells for various treatments.

The present invention relates to a cosmetic composition for whitening, moisturizing, and wrinkle improvement, containing a low-molecular-weight peptide isolated from distiller grains. More specifically, a cosmetic composition is designed in which: a low-molecular peptide having whitening, moisturizing, and wrinkle-improving activities is separated from distiller's grains; according to the present invention, the composition has not only a pigmentation inhibition effect due to a decrease in the expression of tyrosinase-related protein 1 (TRP-1), but also a wrinkle improvement effect due to an increase in the expression of type I collagen (Collagen Type 1) and laminin-5 (Laminin-5), by containing the composition as an active ingredient, and has a moisturizing effect due to an increase in the expression of Filagrin.

Methods comprise exposing a culture of human pluripotent stem cells adherent on a first substrate comprising a first laminin to a differentiation medium for a first time period of 15 days to 50 days to obtain a first population of adherent cells comprising RPE cells and / or progenitors thereof; at the end of the first time period, dissociating the first population from the first substrate; replating the dissociated first population of cells on a second substrate comprising a second laminin; and culturing the replated first population of cells on the second substrate for a second time period to obtain an expanded and matured second population of cells comprising the RPE cells. The first and second laminins are independently selected from LN-521, LN-511, LN-111 and LN-121, and are an intact protein or protein fragment. Also included are RPE cells and materials and compositions utilizing such RPE cells for various treatments.

The present application relates to the technical field of in vitro diagnosis, disclose a kind of laminin determination kit and preparation method thereof, the reagent kit is composed of reagent 1 and reagent 2, the main component of reagent 1 has ammonium chloride, sodium azide, Brij-35, sodium chloride, PEG6000, EDTA2Na, F (ab') 2 fragment, the main component of reagent 2 has mouse monoclonal antibody coated latex microparticle, Tris-Hcl buffer solution, sucrose, gelatin, Tween-20, NP30, Proclin300, mouse monoclonal antibody coated latex microparticle in the reagent kit has good suspensibility and monodispersity, stably exists in aqueous system, improves the determination intensity and stability of signal. The kit is specific, and high accuracy.

The invention provides a serum-free medium for maintaining dryness of mesenchymal stem cells as well as a preparation method and application of the serum-free medium, and belongs to the technical field of biological medicines. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, the basic culture medium is a DMEM / F12 culture medium, and the exogenous additive components comprise bFGF, EGF, PDGF-BB, recombinant human fibronectin, laminin, insulin, transferrin, sodium selenate, beta-mercaptoethanol and ascorbic acid. The serum-free culture medium provided by the invention is a special culture medium for the mesenchymal stem cells, which is completely free of animal-derived components, is suitable for large-scale amplification of clinical-grade cell treatment products, is relatively low in preparation cost and high in stem differentiation capability, and can remarkably improve the proliferation efficiency of the cultured mesenchymal stem cells.

The present disclosure provides, among other things, a class of antibodies or an antigen binding fragments that bind to calf-1 or calf-2 domain of α7β1 integrin. The anti-α7β1 antibodies of the present disclosure are effective in stabilizing the α7β1 integrin in the extended closed and extended-open conformations, which enhances the binding to laminins.

The present application relates to the technical field of artificial dermis, and particularly relates to a preparation method and application of artificial dermis based on biomimetic acellular matrix. The artificial dermis comprises a biomimetic acellular matrix component A and an auxiliary component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, matricellular protein and dextran; the component B comprises antibacterial peptide, antibiotic and high molecular polymer material; the mass ratio of the component A to the component B is (4-6):(1-4). The preparation method comprises the steps of respectively preparing A and B component process mother liquor, step-by-step crosslinking to prepare a hydrogel with a double-layer gradient structure, and then freeze-drying and sterilization treatment. The artificial dermis has good biocompatibility and antibacterial performance, can effectively promote wound healing, and is suitable for repairing acute and chronic wounds of limbs, facial parts and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The invention discloses a polyurethane surface bionic coating capable of synergistically resisting blood coagulation and promoting endothelialization and a preparation method, and relates to the technical field of biomedical materials. According to the invention, tannic acid (TA) and polylysine (PLL) are subjected to a Schiff base and Michael addition reaction in a weak alkaline environment to form a covalent cross-linked bionic intermediate layer, and the intermediate layer synergistically loads heparin (Hep) and laminin (Ln) on the surface of polyurethane (PU) to form a multifunctional active coating, so that the polyurethane material with the bionic coating is formed. The surface coating of the polyurethane material significantly reduces platelet adhesion and hemolysis rate, promotes endothelial cell adhesion, proliferation and functional expression, and inhibits excessive proliferation of smooth muscle cells, and the polyurethane material is simple in preparation process, mild in condition and suitable for surface functional modification of blood contact materials such as artificial blood vessels and interventional catheters.