79 results about "Laminin" patented technology

The invention relates to the field of biological medicine and cosmetics, in particular to laminin RL-20 and application of the laminin RL-20 in cosmetics.

The invention discloses a composition with sensitive skin repairing and anti-aging functions as well as an emulsion and application of the composition, and belongs to the technical field of cosmetics. The composition specifically comprises a pseudoalteromonas fermentation product extract, collagen, palmitoyl tripeptide-5, acetylchitosamine, sodium hyaluronate, silanetriol, caffeine and acetyl hexapeptide-8, and can promote the generation of laminin-5 and integrin beta1, promote the vitality of keratinocytes and fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, and promote the growth of the keratinocytes and the fibroblasts. The generation of inflammatory factors (IL-6) is inhibited; the generation of collagen IV and collagen VII is promoted; the invention further provides an emulsion prepared from the composition, and the optimal formula of the emulsion is obtained through optimization. The active composition disclosed by the invention has a wide application prospect in the field of cosmetics, and particularly has important significance in sensitive skin repair and anti-aging directions.

The invention discloses a model for predicting the progression hepatic fibrosis risk of a WD long-term maintenance treatment patient and a design method thereof, and relates to the technical field of medical information, the prediction model comprises the following steps: an input module receives patient parameter indexes composed of sex (SEX), platelet (PLT), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (Apo-A1), laminin (LN) and copper cyanin (CER), and the input module receives the patient parameter indexes composed of sex (SEX), platelet (PLT), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (Apo-A1), laminin (LN) and copper cyanin (CER); the processing module obtains single scores corresponding to the seven independent risk factors, the single scores are added to obtain a total score, the prediction probability corresponding to the total score is the risk of the progression-stage hepatic fibrosis of the WD long-term maintenance treatment patient, and the prediction risk probability is output through the output module. The column diagram prediction model established by the invention has relatively high accuracy, and can be conveniently used for early recognition and risk prediction of progression-stage hepatic fibrosis of WD long-term maintenance treatment patients.

A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes and method of manufacture. A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes, comprising: a first layer of ADM of biological origin arranged inferiorly to delimit a cavity with a lower edge; a filling material disposed in the cavity surrounded by the first layer of ADM consisting of a ground ADM having the consistency of a fibrous paste; and a second layer of ADM of biological origin arranged on the upper part covering the filling material and whose upper edge rests peripherally on the lower edge of the first layer of ADM, thus defining a rim;where the first layer of ADM and the second layer of ADM are joined by an eyebrow overlap along the upper edge of the first layer of ADM with the lower edge of the peripheral rim using a biocompatible adhesive, or by absorbable suture; where the first layer of ADM, the second layer of ADM and the ADM filling material are not immunogenic; and where the ADM comprises collagen fibers, fibronectin, elastin, laminin, glycosaminoglycans and hyaluronic acid. Method for its manufacture.

The invention discloses a biomarker for monitoring the curative effect of a PD-1 antibody of a PD-L1Positive gastric cancer patient, and relates to the technical field of biomedical detection, and the biomarker is laminin gamma 2. According to the invention, the laminin gamma2 is used as a novel biomarker for monitoring the curative effect of the PD-1 treatment antibody of the PD-L1Positive gastric cancer patient, experiments prove that the serum level of the laminin gamma2 is higher than that of healthy people in the gastric cancer patient and is in positive correlation with a prognosis related factor sPD-L1, the tissue expression of the PD-L1Positive patient is higher, and the serum level of a person without PD-1 antibody treatment response is higher; it is proved that the marker has good progress monitoring value, treatment reaction differences can be effectively distinguished, and the blank of lack of PD-1 antibody curative effect monitoring markers for patients of the type is filled up.

The invention belongs to the technical field of animal reproductive biology and cell engineering, and particularly relates to a fluorescence labeling expression system for visualizing an oocyte nucleus maturation process and application of the fluorescence labeling expression system. The fluorescence labeling expression system comprises an eukaryotic expression vector pVenus-LMNB1; the eukaryotic expression vector pVenus-LMNB1 comprises a nucleotide sequence for coding the fusion protein Venus-LMNB1. According to the invention, a nuclear fiber layer protein stably expressed on a nuclear membrane is selected as a research object, a eukaryotic expression vector pVenus-LMNB1 is constructed by using a molecular cloning technology, an LMNB1 gene containing a green fluorescent label Venus is overexpressed in a porcine oocyte by using a microinjection technology, fluorescent protein labeling of a structural protein on the nuclear membrane is utilized, and the expression vector pVenus-LMNB1 is constructed. The expression positioning condition of the LMNB1 in the maturation process of the porcine oocytes is observed, and a powerful tool is provided for development of in-vitro culture related researches of the porcine oocytes.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The present disclosure describes methods of maintaining the phenotype of differentiated cells. Generally, the natural environment of the body is replicated for the differentiated cell. The differentiated cell is plated on a cell culture substrate comprising a laminin, such as laminin-521 or laminin-511. The substrate may also contain a cadherin. This maintains the phenotype of the differentiated cell.

Methods comprise exposing a culture of human pluripotent stem cells adherent on a first substrate comprising a first laminin to a differentiation medium for a first time period of 15 days to 50 days to obtain a first population of adherent cells comprising RPE cells and / or progenitors thereof; at the end of the first time period, dissociating the first population from the first substrate; replating the dissociated first population of cells on a second substrate comprising a second laminin; and culturing the replated first population of cells on the second substrate for a second time period to obtain an expanded and matured second population of cells comprising the RPE cells. The first and second laminins are independently selected from LN-521, LN-511, LN-111 and LN-121, and are an intact protein or protein fragment. Also included are RPE cells and materials and compositions utilizing such RPE cells for various treatments.

The present application relates to the technical field of in vitro diagnosis, disclose a kind of laminin determination kit and preparation method thereof, the reagent kit is composed of reagent 1 and reagent 2, the main component of reagent 1 has ammonium chloride, sodium azide, Brij-35, sodium chloride, PEG6000, EDTA2Na, F (ab') 2 fragment, the main component of reagent 2 has mouse monoclonal antibody coated latex microparticle, Tris-Hcl buffer solution, sucrose, gelatin, Tween-20, NP30, Proclin300, mouse monoclonal antibody coated latex microparticle in the reagent kit has good suspensibility and monodispersity, stably exists in aqueous system, improves the determination intensity and stability of signal. The kit is specific, and high accuracy.

The invention provides a serum-free medium for maintaining dryness of mesenchymal stem cells as well as a preparation method and application of the serum-free medium, and belongs to the technical field of biological medicines. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, the basic culture medium is a DMEM / F12 culture medium, and the exogenous additive components comprise bFGF, EGF, PDGF-BB, recombinant human fibronectin, laminin, insulin, transferrin, sodium selenate, beta-mercaptoethanol and ascorbic acid. The serum-free culture medium provided by the invention is a special culture medium for the mesenchymal stem cells, which is completely free of animal-derived components, is suitable for large-scale amplification of clinical-grade cell treatment products, is relatively low in preparation cost and high in stem differentiation capability, and can remarkably improve the proliferation efficiency of the cultured mesenchymal stem cells.

The present disclosure provides, among other things, a class of antibodies or an antigen binding fragments that bind to calf-1 or calf-2 domain of α7β1 integrin. The anti-α7β1 antibodies of the present disclosure are effective in stabilizing the α7β1 integrin in the extended closed and extended-open conformations, which enhances the binding to laminins.

The present application relates to the technical field of artificial dermis, and particularly relates to a preparation method and application of artificial dermis based on biomimetic acellular matrix. The artificial dermis comprises a biomimetic acellular matrix component A and an auxiliary component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, matricellular protein and dextran; the component B comprises antibacterial peptide, antibiotic and high molecular polymer material; the mass ratio of the component A to the component B is (4-6):(1-4). The preparation method comprises the steps of respectively preparing A and B component process mother liquor, step-by-step crosslinking to prepare a hydrogel with a double-layer gradient structure, and then freeze-drying and sterilization treatment. The artificial dermis has good biocompatibility and antibacterial performance, can effectively promote wound healing, and is suitable for repairing acute and chronic wounds of limbs, facial parts and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The invention discloses a polyurethane surface bionic coating capable of synergistically resisting blood coagulation and promoting endothelialization and a preparation method, and relates to the technical field of biomedical materials. According to the invention, tannic acid (TA) and polylysine (PLL) are subjected to a Schiff base and Michael addition reaction in a weak alkaline environment to form a covalent cross-linked bionic intermediate layer, and the intermediate layer synergistically loads heparin (Hep) and laminin (Ln) on the surface of polyurethane (PU) to form a multifunctional active coating, so that the polyurethane material with the bionic coating is formed. The surface coating of the polyurethane material significantly reduces platelet adhesion and hemolysis rate, promotes endothelial cell adhesion, proliferation and functional expression, and inhibits excessive proliferation of smooth muscle cells, and the polyurethane material is simple in preparation process, mild in condition and suitable for surface functional modification of blood contact materials such as artificial blood vessels and interventional catheters.

Disclosed are compositions and methods for their use that can be used in cosmetic applications. The composition can include an effective amount of a Centella asiatica stem cells to reduce the activity of hyaluronidase in skin, an effective amount of tetradecyl aminobutyroylvalylamino butyric urea trifluoroacetate or Alpinia galanga leaf extract to promote the production of hyaluronic acid in skin, an effective amount of tripeptide-1 to promote the production of fibronectin and laminin in skin, and a dermatologically acceptable vehicle.

The invention provides a molecular self-assembly integrin patch for regulating and controlling mechanical signals of cells as well as a preparation method and application of the molecular self-assembly integrin patch, and an extracellular matrix (ECM) bionic cell patch is constructed through a molecular self-assembly technology. According to the patch, a laminin-derived integrin binding ligand is extended and self-assembled through an N-terminal aromatic amino acid to form a fibrous nano network, and the patch has controllable nano-scale ligand distribution, network viscoelasticity and stability, and can specifically activate integrin beta1 at the top end of MSC (mesenchymal stem cell), so that a mechanical signal is conducted from a cell membrane to a cell nucleus. The process coordinates cytoskeleton reconstruction, nuclear deformation and chromatin rearrangement, and finally drives the MSC to efficiently differentiate into neuron-like cells under the condition of no exogenous gene or chemical induction, so as to express nerve markers such as TUBB3 and present functional characteristics such as spontaneous calcium transient. The cell patch can be widely applied to nerve regeneration, tissue engineering, disease model construction and intelligent biological material development.

The present invention relates to the technical field of biomedicine, and specifically relates to a modified laminin. By attaching an amino acid linker to the N-terminus of laminin, the interactions between the laminin and culture plates and between the laminin and cells are enhanced, the adherent activity of cells is improved, thereby achieving suspension culture without the need for pre-coating in the cell culture process.

The invention is applied to the technical field of medicine of nano-drugs for treatment, and particularly discloses preparation of a polydopamine nanocomposite and application of the polydopamine nanocomposite in treatment of endometriosis, the polydopamine nanocomposite is polydopamine nanoparticles which are modified by polyethylene glycol and laminin and carry didrogesterone, and the polydopamine nanoparticles are polydopamine nanoparticles which are modified by polyethylene glycol and laminin and carry didrogesterone. The disease is endometriosis. Polydopamine nano-particles which are modified by polyethylene glycol and laminin and carry didrogesterone are constructed and applied to in-vitro and in-vivo experiments related to treatment of endometriosis, and the polydopamine nano-particles have the effects of improving oxidative stress, inhibiting inflammatory microenvironment, promoting ectopic endometrial atrophy, inhibiting inflammation and treating endometriosis. And a good treatment effect is achieved in the aspect of reducing the dosage of the didrogesterone.

The invention relates to the technical field of cosmetics, in particular to polypeptide essence with an anti-alopecia function and a preparation method of the polypeptide essence. The polypeptide composite component is formed by mixing acetyl tetrapeptide-3 and oligopeptide-54 according to the mass ratio of 1: (1-3). The acetyl tetrapeptide-3 can activate fibroblasts, promote synthesis of laminin, III type collagen and VII type collagen, reinforce an epidermis-dermis connecting structure, reduce the hair loss rate and prolong the hair growth period; the oligopeptide-54 can lower hair loss related signal molecules such as DKK-1, stimulate hair follicle activity, promote melanogenesis and improve the problem of premature graying of hair. The combination of the two can synergistically improve the anti-hair loss effect from different action targets, experiments prove that the polypeptide activity in the proportion range is the highest, the excessive addition easily causes component agglomeration and reduces bioavailability, and the expected effect cannot be achieved if the addition is insufficient. The invention has the beneficial effect of high-efficiency hair loss prevention: the polypeptide and the plant extract have a synergistic effect, and the problem of hair loss is solved from multiple dimensions of activating hair follicles, strengthening hair anchoring and improving scalp environment.

Provided are preparation methods for an islet extracellular matrix scaffold and a biomimetic islet organoid. The scaffold is a gel, and the gel contains 3-5 mg / mL Collagen I, 3-5 mg / mL Collagen IV, and 0.8-1.2 mg / mL Laminin. For the scaffold, Collagen I, Collagen IV, and Laminin are simultaneously selected as extracellular matrix components, which have relatively strong interactions with islet cells, and effectively promote the islet cells and enhance insulin secretion function stimulated by glucose after binding to the islet cells. Collagen VI can also be used. Collagen VI can act on the islet cells to effectively enhance the survival of the islet cells and the insulin secretion function stimulated by glucose. The gel can form a relatively intact network structure without needing to use a cross-linking agent, thereby enhancing the support capacity for the islet organoid.

The invention belongs to the technical field of biomedicine, and provides a Lam-pGelMA microsphere aiming at the technical problems existing in the traditional GelMA microsphere, which comprises a GelMA microsphere with a porous structure and a laminin coating with a functionally modified surface. The invention further provides a preparation method of the Lam-pGelMA microspheres. The preparation method comprises the following steps: S1, preparing the GelMA microspheres; s2, preparation of GelMA microspheres of a porous structure; and S3, preparation of Lam-pGelMA microspheres. The Lam-pGelMA microsphere synthesized by the invention has a porous structure, and the surface of the Lam-pGelMA microsphere is functionally modified by laminin, so that a better attachment site can be provided for the growth of NSCs to promote the adhesion growth of the NSCs; and the laminin functional modification can significantly enhance the survival and proliferation activity of the NSCs in the damaged environment.

The invention is directed to a composition of soluble placental ECM in storage solution and the methods of manufacture related thereto. The solubilized placental ECM solution is manufactured through a process of partitioning placental ECM from the placental tissue in a high acidic pH environment and performing an ultrafiltration step and diafiltration step through a tangential flow filtration system to increase the pH. The soluble placental ECM retains the native characteristics of placental ECM components and includes collagens (types I, III, V, VI, IX, and X), elastin, laminin, and fibronectin.

The disclosure provides compositions and uses of various RGD-like peptides that interact with laminin-332 and have a minimal sequence of KADA-4 (SEQ ID NO:9), RADA-4 (SEQ ID NO:8), RFDA-4 (SEQ ID NO:22), or RADF-4 (SEQ ID NO:25). The uses include treatment of skin and mucous membrane wounds as well as cosmetic uses of the aforementioned peptides.

The invention relates to a circular non-coding RNA (Ribonucleic Acid) sno-circCNOT1 and application thereof, and belongs to the technical field of medicine. The invention provides a circular non-coding RNA (Ribonucleic Acid), the nucleotide sequence of the circular non-coding RNA is shown as SEQ ID NO: 1, and the circular non-coding RNA is named as sno-circCNOT1. The invention identifies a novel snoRNA-like annular non-coding RNA, the novel snoRNA-like annular non-coding RNA is named as sno-circCNOT1, the expression of the novel snoRNA-like annular non-coding RNA is adjusted by shear force, and the novel snoRNA-like annular non-coding RNA can promote NLRP3-dependent inflammatory response and pyroptosis of endothelial cells by combining nucleofibrin and stabilizing METTL14 protein, thereby aggravating blood flow shear force and atherosclerosis induced by high fat diet. Therefore, the compound can be used as a target for relieving inflammatory response and pyroptosis of endothelial cells and treating atherosclerosis.

The invention discloses a 3D skin model based on photocuring bio-printing and a construction method thereof, and the construction method comprises the following steps: A, printing bio-ink by adopting a 3D printing technology to obtain a corium layer; b, inoculating keratinocytes or epidermal stem cells to the surface of a corium layer, standing for a certain time, and then carrying out immersion culture; c, the dermis layer obtained after immersion culture is completed is added into a gas-liquid culture medium for gas-liquid culture, and the 3D skin model is obtained; wherein laminin is added into the gas-liquid culture medium in the step C. By adopting the construction scheme of combining 3D printing of the corium layer with artificial culture of the epidermal layer, the batch stability can be improved, the artificial culture period can be shortened, the cost can be reduced, and the quality of the model can be improved; the epidermal layer and the corium layer of the prepared 3D skin model are tightly connected, the epidermal layer is completely differentiated, and the 3D skin model can be widely applied to safety evaluation and efficacy evaluation of cosmetics or drugs.

A method for preparing a bionic injectable polypeptide hydrogel is provided. The hydrogel is formed using a brain extracellular matrix laminin-derived peptide DDIKVAV modified with 9-fluorenylmethoxycarbonyl (Fmoc) (Fmoc-DDIKVAV) and an immunostimulatory peptide FTKPRF modified with Fmoc (Fmoc-FTKPRF) as hydrogel monomers. These monomers further utilize non-covalent bond forces such as hydrogen bonds, hydrophobic interactions, and x-x stacking to co-assemble into a hydrogel within a short time at 37° C. The bionic hybrid polypeptide hydrogel is injectable and can serve as a drug reservoir implanted into cavities formed after tumor surgery. The drugs loaded in the hydrogel are slowly released at a stable and controlled rate and appropriate concentration within the post-surgical cavity, thereby effectively killing residual tumor cells while avoiding the toxic side effects of systemic drug administration.

A vascular plant-based cellulosic scaffold for use in the production or growth of neural stem cells in vitro is provided. The scaffold can be obtained by decellularizing a vascular plant or a part thereof, and can support the growth of neural stem cells. The scaffold can be coated with any traditional coating known in the art, such as biomolecules, synthetic biomolecules (poly-L-ornithine), ligands, proteins, amino acids, antibodies, extracellular matrix, fibrin, laminin, collagen, fibronectin, poly-l-lysine, small molecules, hydrogels or matrigels. Also provided is an in vitro method of growing or producing neural stem cells.

The invention provides a self-assembled polypeptide derivative, a hydrogel preparation, a preparation method and application, and particularly belongs to the technical field of biomedical materials. The structure of the self-assembled polypeptide derivative disclosed by the invention is Biotin-DFYIGSSSR, wherein Biotin is a blocked end, DF is D-configuration phenylalanine, YIG is an active sequence derived from laminin, and SSSR is an active amino acid sequence of IGF-1. The self-assembled polypeptide nano hydrogel prepared from the self-assembled polypeptide disclosed by the invention has thixotropy and self-repairing performance, and is resistant to enzymolysis and lasting in biological activity. And long-time residence in damaged tissues can be realized, so that the bioavailability is improved, and tissue repair is promoted. The self-assembled polypeptide derivative hydrogel disclosed by the invention has good biocompatibility and treatment effect, and can be safely used.

The invention discloses application of hydrogel in preparation of an anti-infection and anti-scar repair external preparation for treating and chemotherapy skin wounds, and belongs to the field of biomedical materials. The hydrogel comprises sodium alginate and a copper ion solution, the mass fraction of the copper ion solution is 0.01%-0.05%, and 0.1%-1% of sodium alginate is added into every 100 mL of the copper ion solution according to the mass fraction; the external preparation realizes skin wound infection resistance and scar inhibition after chemotherapy by regulating and controlling the expression of collagen and basement membrane protein in wound tissues. According to the application, bacterial contamination can be effectively inhibited, up-regulation of collagen III and Laminin beta1 is promoted, abnormal hyperplasia of collagen I and collagen VII is inhibited, a soft and regular reticular collagen structure is formed on a wound surface, and HE dyeing shows that dermal fibers are uniform and ordered in direction, and scar tissues are obviously reduced.

The present disclosure provides, among other things, a class of antibodies or an antigen binding fragments that bind to calf-1 or calf-2 domain of α7β1 integrin. The anti-α7β1 antibodies of the present disclosure are effective in stabilizing the α7β1 integrin in the extended closed and extended-open conformations, which enhances the binding to laminins.