47 results about "Laminin" patented technology

A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes and method of manufacture. A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes, comprising: a first layer of ADM of biological origin arranged inferiorly to delimit a cavity with a lower edge; a filling material disposed in the cavity surrounded by the first layer of ADM consisting of a ground ADM having the consistency of a fibrous paste; and a second layer of ADM of biological origin arranged on the upper part covering the filling material and whose upper edge rests peripherally on the lower edge of the first layer of ADM, thus defining a rim;where the first layer of ADM and the second layer of ADM are joined by an eyebrow overlap along the upper edge of the first layer of ADM with the lower edge of the peripheral rim using a biocompatible adhesive, or by absorbable suture; where the first layer of ADM, the second layer of ADM and the ADM filling material are not immunogenic; and where the ADM comprises collagen fibers, fibronectin, elastin, laminin, glycosaminoglycans and hyaluronic acid. Method for its manufacture.

The invention belongs to the technical field of animal reproductive biology and cell engineering, and particularly relates to a fluorescence labeling expression system for visualizing an oocyte nucleus maturation process and application of the fluorescence labeling expression system. The fluorescence labeling expression system comprises an eukaryotic expression vector pVenus-LMNB1; the eukaryotic expression vector pVenus-LMNB1 comprises a nucleotide sequence for coding the fusion protein Venus-LMNB1. According to the invention, a nuclear fiber layer protein stably expressed on a nuclear membrane is selected as a research object, a eukaryotic expression vector pVenus-LMNB1 is constructed by using a molecular cloning technology, an LMNB1 gene containing a green fluorescent label Venus is overexpressed in a porcine oocyte by using a microinjection technology, fluorescent protein labeling of a structural protein on the nuclear membrane is utilized, and the expression vector pVenus-LMNB1 is constructed. The expression positioning condition of the LMNB1 in the maturation process of the porcine oocytes is observed, and a powerful tool is provided for development of in-vitro culture related researches of the porcine oocytes.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

Methods comprise exposing a culture of human pluripotent stem cells adherent on a first substrate comprising a first laminin to a differentiation medium for a first time period of 15 days to 50 days to obtain a first population of adherent cells comprising RPE cells and / or progenitors thereof; at the end of the first time period, dissociating the first population from the first substrate; replating the dissociated first population of cells on a second substrate comprising a second laminin; and culturing the replated first population of cells on the second substrate for a second time period to obtain an expanded and matured second population of cells comprising the RPE cells. The first and second laminins are independently selected from LN-521, LN-511, LN-111 and LN-121, and are an intact protein or protein fragment. Also included are RPE cells and materials and compositions utilizing such RPE cells for various treatments.

The present application relates to the technical field of in vitro diagnosis, disclose a kind of laminin determination kit and preparation method thereof, the reagent kit is composed of reagent 1 and reagent 2, the main component of reagent 1 has ammonium chloride, sodium azide, Brij-35, sodium chloride, PEG6000, EDTA2Na, F (ab') 2 fragment, the main component of reagent 2 has mouse monoclonal antibody coated latex microparticle, Tris-Hcl buffer solution, sucrose, gelatin, Tween-20, NP30, Proclin300, mouse monoclonal antibody coated latex microparticle in the reagent kit has good suspensibility and monodispersity, stably exists in aqueous system, improves the determination intensity and stability of signal. The kit is specific, and high accuracy.

The invention provides a serum-free medium for maintaining dryness of mesenchymal stem cells as well as a preparation method and application of the serum-free medium, and belongs to the technical field of biological medicines. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, the basic culture medium is a DMEM / F12 culture medium, and the exogenous additive components comprise bFGF, EGF, PDGF-BB, recombinant human fibronectin, laminin, insulin, transferrin, sodium selenate, beta-mercaptoethanol and ascorbic acid. The serum-free culture medium provided by the invention is a special culture medium for the mesenchymal stem cells, which is completely free of animal-derived components, is suitable for large-scale amplification of clinical-grade cell treatment products, is relatively low in preparation cost and high in stem differentiation capability, and can remarkably improve the proliferation efficiency of the cultured mesenchymal stem cells.

The present application relates to the technical field of artificial dermis, and particularly relates to a preparation method and application of artificial dermis based on biomimetic acellular matrix. The artificial dermis comprises a biomimetic acellular matrix component A and an auxiliary component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, matricellular protein and dextran; the component B comprises antibacterial peptide, antibiotic and high molecular polymer material; the mass ratio of the component A to the component B is (4-6):(1-4). The preparation method comprises the steps of respectively preparing A and B component process mother liquor, step-by-step crosslinking to prepare a hydrogel with a double-layer gradient structure, and then freeze-drying and sterilization treatment. The artificial dermis has good biocompatibility and antibacterial performance, can effectively promote wound healing, and is suitable for repairing acute and chronic wounds of limbs, facial parts and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The invention discloses a polyurethane surface bionic coating capable of synergistically resisting blood coagulation and promoting endothelialization and a preparation method, and relates to the technical field of biomedical materials. According to the invention, tannic acid (TA) and polylysine (PLL) are subjected to a Schiff base and Michael addition reaction in a weak alkaline environment to form a covalent cross-linked bionic intermediate layer, and the intermediate layer synergistically loads heparin (Hep) and laminin (Ln) on the surface of polyurethane (PU) to form a multifunctional active coating, so that the polyurethane material with the bionic coating is formed. The surface coating of the polyurethane material significantly reduces platelet adhesion and hemolysis rate, promotes endothelial cell adhesion, proliferation and functional expression, and inhibits excessive proliferation of smooth muscle cells, and the polyurethane material is simple in preparation process, mild in condition and suitable for surface functional modification of blood contact materials such as artificial blood vessels and interventional catheters.

Disclosed are compositions and methods for their use that can be used in cosmetic applications. The composition can include an effective amount of a Centella asiatica stem cells to reduce the activity of hyaluronidase in skin, an effective amount of tetradecyl aminobutyroylvalylamino butyric urea trifluoroacetate or Alpinia galanga leaf extract to promote the production of hyaluronic acid in skin, an effective amount of tripeptide-1 to promote the production of fibronectin and laminin in skin, and a dermatologically acceptable vehicle.

The invention provides a molecular self-assembly integrin patch for regulating and controlling mechanical signals of cells as well as a preparation method and application of the molecular self-assembly integrin patch, and an extracellular matrix (ECM) bionic cell patch is constructed through a molecular self-assembly technology. According to the patch, a laminin-derived integrin binding ligand is extended and self-assembled through an N-terminal aromatic amino acid to form a fibrous nano network, and the patch has controllable nano-scale ligand distribution, network viscoelasticity and stability, and can specifically activate integrin beta1 at the top end of MSC (mesenchymal stem cell), so that a mechanical signal is conducted from a cell membrane to a cell nucleus. The process coordinates cytoskeleton reconstruction, nuclear deformation and chromatin rearrangement, and finally drives the MSC to efficiently differentiate into neuron-like cells under the condition of no exogenous gene or chemical induction, so as to express nerve markers such as TUBB3 and present functional characteristics such as spontaneous calcium transient. The cell patch can be widely applied to nerve regeneration, tissue engineering, disease model construction and intelligent biological material development.

The present invention relates to the technical field of biomedicine, and specifically relates to a modified laminin. By attaching an amino acid linker to the N-terminus of laminin, the interactions between the laminin and culture plates and between the laminin and cells are enhanced, the adherent activity of cells is improved, thereby achieving suspension culture without the need for pre-coating in the cell culture process.

The invention relates to the technical field of cosmetics, in particular to polypeptide essence with an anti-alopecia function and a preparation method of the polypeptide essence. The polypeptide composite component is formed by mixing acetyl tetrapeptide-3 and oligopeptide-54 according to the mass ratio of 1: (1-3). The acetyl tetrapeptide-3 can activate fibroblasts, promote synthesis of laminin, III type collagen and VII type collagen, reinforce an epidermis-dermis connecting structure, reduce the hair loss rate and prolong the hair growth period; the oligopeptide-54 can lower hair loss related signal molecules such as DKK-1, stimulate hair follicle activity, promote melanogenesis and improve the problem of premature graying of hair. The combination of the two can synergistically improve the anti-hair loss effect from different action targets, experiments prove that the polypeptide activity in the proportion range is the highest, the excessive addition easily causes component agglomeration and reduces bioavailability, and the expected effect cannot be achieved if the addition is insufficient. The invention has the beneficial effect of high-efficiency hair loss prevention: the polypeptide and the plant extract have a synergistic effect, and the problem of hair loss is solved from multiple dimensions of activating hair follicles, strengthening hair anchoring and improving scalp environment.

Provided are preparation methods for an islet extracellular matrix scaffold and a biomimetic islet organoid. The scaffold is a gel, and the gel contains 3-5 mg / mL Collagen I, 3-5 mg / mL Collagen IV, and 0.8-1.2 mg / mL Laminin. For the scaffold, Collagen I, Collagen IV, and Laminin are simultaneously selected as extracellular matrix components, which have relatively strong interactions with islet cells, and effectively promote the islet cells and enhance insulin secretion function stimulated by glucose after binding to the islet cells. Collagen VI can also be used. Collagen VI can act on the islet cells to effectively enhance the survival of the islet cells and the insulin secretion function stimulated by glucose. The gel can form a relatively intact network structure without needing to use a cross-linking agent, thereby enhancing the support capacity for the islet organoid.

The invention is directed to a composition of soluble placental ECM in storage solution and the methods of manufacture related thereto. The solubilized placental ECM solution is manufactured through a process of partitioning placental ECM from the placental tissue in a high acidic pH environment and performing an ultrafiltration step and diafiltration step through a tangential flow filtration system to increase the pH. The soluble placental ECM retains the native characteristics of placental ECM components and includes collagens (types I, III, V, VI, IX, and X), elastin, laminin, and fibronectin.

The disclosure provides compositions and uses of various RGD-like peptides that interact with laminin-332 and have a minimal sequence of KADA-4 (SEQ ID NO:9), RADA-4 (SEQ ID NO:8), RFDA-4 (SEQ ID NO:22), or RADF-4 (SEQ ID NO:25). The uses include treatment of skin and mucous membrane wounds as well as cosmetic uses of the aforementioned peptides.

The invention relates to a circular non-coding RNA (Ribonucleic Acid) sno-circCNOT1 and application thereof, and belongs to the technical field of medicine. The invention provides a circular non-coding RNA (Ribonucleic Acid), the nucleotide sequence of the circular non-coding RNA is shown as SEQ ID NO: 1, and the circular non-coding RNA is named as sno-circCNOT1. The invention identifies a novel snoRNA-like annular non-coding RNA, the novel snoRNA-like annular non-coding RNA is named as sno-circCNOT1, the expression of the novel snoRNA-like annular non-coding RNA is adjusted by shear force, and the novel snoRNA-like annular non-coding RNA can promote NLRP3-dependent inflammatory response and pyroptosis of endothelial cells by combining nucleofibrin and stabilizing METTL14 protein, thereby aggravating blood flow shear force and atherosclerosis induced by high fat diet. Therefore, the compound can be used as a target for relieving inflammatory response and pyroptosis of endothelial cells and treating atherosclerosis.

A method for preparing a bionic injectable polypeptide hydrogel is provided. The hydrogel is formed using a brain extracellular matrix laminin-derived peptide DDIKVAV modified with 9-fluorenylmethoxycarbonyl (Fmoc) (Fmoc-DDIKVAV) and an immunostimulatory peptide FTKPRF modified with Fmoc (Fmoc-FTKPRF) as hydrogel monomers. These monomers further utilize non-covalent bond forces such as hydrogen bonds, hydrophobic interactions, and x-x stacking to co-assemble into a hydrogel within a short time at 37° C. The bionic hybrid polypeptide hydrogel is injectable and can serve as a drug reservoir implanted into cavities formed after tumor surgery. The drugs loaded in the hydrogel are slowly released at a stable and controlled rate and appropriate concentration within the post-surgical cavity, thereby effectively killing residual tumor cells while avoiding the toxic side effects of systemic drug administration.

A vascular plant-based cellulosic scaffold for use in the production or growth of neural stem cells in vitro is provided. The scaffold can be obtained by decellularizing a vascular plant or a part thereof, and can support the growth of neural stem cells. The scaffold can be coated with any traditional coating known in the art, such as biomolecules, synthetic biomolecules (poly-L-ornithine), ligands, proteins, amino acids, antibodies, extracellular matrix, fibrin, laminin, collagen, fibronectin, poly-l-lysine, small molecules, hydrogels or matrigels. Also provided is an in vitro method of growing or producing neural stem cells.

The invention provides a self-assembled polypeptide derivative, a hydrogel preparation, a preparation method and application, and particularly belongs to the technical field of biomedical materials. The structure of the self-assembled polypeptide derivative disclosed by the invention is Biotin-DFYIGSSSR, wherein Biotin is a blocked end, DF is D-configuration phenylalanine, YIG is an active sequence derived from laminin, and SSSR is an active amino acid sequence of IGF-1. The self-assembled polypeptide nano hydrogel prepared from the self-assembled polypeptide disclosed by the invention has thixotropy and self-repairing performance, and is resistant to enzymolysis and lasting in biological activity. And long-time residence in damaged tissues can be realized, so that the bioavailability is improved, and tissue repair is promoted. The self-assembled polypeptide derivative hydrogel disclosed by the invention has good biocompatibility and treatment effect, and can be safely used.

The invention discloses application of hydrogel in preparation of an anti-infection and anti-scar repair external preparation for treating and chemotherapy skin wounds, and belongs to the field of biomedical materials. The hydrogel comprises sodium alginate and a copper ion solution, the mass fraction of the copper ion solution is 0.01%-0.05%, and 0.1%-1% of sodium alginate is added into every 100 mL of the copper ion solution according to the mass fraction; the external preparation realizes skin wound infection resistance and scar inhibition after chemotherapy by regulating and controlling the expression of collagen and basement membrane protein in wound tissues. According to the application, bacterial contamination can be effectively inhibited, up-regulation of collagen III and Laminin beta1 is promoted, abnormal hyperplasia of collagen I and collagen VII is inhibited, a soft and regular reticular collagen structure is formed on a wound surface, and HE dyeing shows that dermal fibers are uniform and ordered in direction, and scar tissues are obviously reduced.

Proposed is a method for replacement therapy for damaged skin using polymeric collagen-laminin matrices which are histotypically similar to tissues of the body and contain biologically active agents in the form of cellular derivatives, namely collagens and laminins, which promote the structure-forming function of the damaged area. The subject of the invention is a new and effective method for producing such a matrix with a wound-healing effect, said method making it possible to reduce the toxic load and improve the final product yield, as well as improve the quality of the purification and rinsing of the collagen-laminin matrix. The collagen-laminin matrix produced using the method according to the invention is a safe and effective material for regenerative medicine (including for the healing of ulcers, burns, wounds and skin defects), and one which does not require frequent replacement throughout the healing period. It also holds promise for use in clinical practice for the treatment of surgical, traumatic or chronic wounds, as well as burns.

The invention relates to the technical field of biomedical materials, and discloses a freeze-dried matrigel and a preparation method and application thereof, and the freeze-dried matrigel is prepared by dissolving a natural basement membrane extract in a high-glucose DMEM (Dulbecco Modified Eagle Medium); the natural basement membrane extract is derived from EHS mouse tumor tissue rich in extracellular matrix proteins. The freeze-dried matrigel provided by the invention is prepared by taking a natural basement membrane extract as a core active component through an optimized freeze-drying process. After redissolution, a three-dimensional fiber network structure can be quickly recovered, the biological functions of active ingredients such as collagen IV, laminin and nest protein in the basement membrane are reserved, and the storage stability is high; meanwhile, the matrix gel has the advantages of good redissolution effect and good cell compatibility, can be widely applied to the fields of cell culture and in-vitro disease model construction, and solves the technical problems that the traditional liquid matrix gel needs to be stored at low temperature, is high in transportation cost and is easy to degrade and inactivate.

Provided is a technology allowing for stable supply of brain microvascular endothelium-like cells. This coating agent for inducing differentiation of pluripotent stem cells into brain microvascular endothelium-like cells contains at least one component of a Laminin-221 fragment or an N-terminal Vitronectin.

The application discloses a high-throughput and automatic electrochemical addressing sensing array, which is obtained by constructing a micropore array on a laser-induced graphene electrode film, obtaining a laser-induced graphene-based electrode array with micropores, then sequentially modifying titanium dioxide single crystals, platinum nanoparticles and laminin in the micropores of the laser-induced graphene-based electrode array to obtain an LIG-TiO2-Pt-Lam micropore electrode array, using the LIG-TiO2-Pt-Lam micropore electrode array as a working electrode, using multiple micropores for incubating tumor cells of the same type or different types, connecting a robot hand integrated with a reference electrode and a counter electrode to realize automatic screening of anticancer drugs. Through setting a moving track of the robot hand, electric addressing pulse signals generated by cells in each micropore after adding candidate anticancer drugs for incubation and releasing H2O2 are collected, and a computer or an instrument device is used for data storage and processing, so that high-throughput, automatic and accurate screening of the candidate anticancer drugs is realized.

To provide a novel composition for application on the skin of human or the like that prevents the deterioration of skin function.SOLUTION: The present invention provides a composition that comprises tranexamic acid for inhibiting the degradation of laminin 511 in the skin.SELECTED DRAWING: None

The disclosure provides compositions and uses of various RGD-likc peptides that interact with laminin-332 and have a minimal sequence of KADA-4 (SEQ ID NO:9), RADA-4 (SEQ ID NO:8), RFDA-4 (SEQ ID NO:22), or RADF-4 (SEQ ID NO:25). The uses include treatment of skin and mucous membrane wounds as well as cosmetic uses of the aforementioned peptides.

The present application relates to a kind of laminin or its specific fragment polypeptide, the complex formed by non-covalent adsorption immunological factor with it and its application in biomaterials.38 polypeptides screened by the present application. One or more of the complete laminin containing the polypeptide screened by the present application, or complete fibronectin is used as carrier protein, and is mixed with biomaterial base, immunological factor etc. to make complex, because there is non-covalent adsorption between carrier protein and immunological factor, immunological factor can be biomimetic adsorption carried on the material combined with carrier protein. The product containing the complex of the present application can automatically dissociate and release immunological factor in neutral environment and maintain the slow release for several days.Moreover, the product made of the complex of the present application can avoid activating the immune response of the implant, solve the problem that transgenic expression vector enters the patient's body and easily induces intense immune response, difficult to realize secondary administration etc.

The invention relates to the use of a laminin (LN) matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human totipotent cells, (ii) culturing the population on a laminin-coated support in an endoderm stimulation medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a laminin-coated support in a hepatic stimulation medium to produce a population of human hepatoblast-like cells, and (iv) optionally culturing said population of human hepatoblast-like cells on a laminin-coated support in a hepatic maturation medium to produce a population of human hepatocyte-like cells.The invention further relates to a population of human hepatoblast-type cells or human embryonic hepatocyte-type cells obtained by the method of the invention. The invention further relates to a population of human hepatoblast-type cells expressing HNF4 α and expressing substantially AFP for use in a method of treating the human body.

A method for functionalizing a hollow fiber membrane for cell expansion, differentiation, harvesting, activation, or any combination thereof of target cells (e.g., natural killer cells) includes contacting a biotinylated molecule to a surface of the hollow fiber membrane including an extracellular matrix component, the biotinylated molecule binding to the extracellular matrix component and having an affinity for the target cells. The biotinylated molecule is selected from the group consisting of: cytokine, epitope, ligand, monoclonal antibody, stains, aptamer, and combinations thereof. The extracellular matrix component selected from the group consisting of: fibronectin, vitronectin, fibrinogen, collagen, laminin, streptavidin, truncations thereof, and combinations thereof.

This invention discloses a method and application for rapidly preparing humanized collagen using umbilical cord mesenchymal stem cell material, belonging to the field of biomaterial preparation technology. The key technical points of the method are as follows: S1, processing and passage-expanding umbilical cord tissue; S2, seeding the expanded umbilical cord mesenchymal stem cell suspension into a culture dish, then adding DMEM / F12 medium containing platelet lysis buffer for culture, followed by induction medium to obtain extracellular matrix; S3, enzymatically hydrolyzing the extracellular matrix, centrifuging the hydrolysate to obtain the supernatant, adjusting the pH, allowing it to stand, centrifuging again to obtain the precipitate, reconstituteing the precipitate, purifying it, centrifuging again, and then lyophilizing it; S4, forming the formulation to achieve efficient secretion and self-assembly of collagen, while utilizing extracellular matrix components (such as fibronectin and laminin) secreted by umbilical cord mesenchymal stem cells to assist collagen in forming an active product with a natural triple helix structure.