198 results about "Epidermal growth factor" patented technology

The invention relates to the technical field of medical dressings, in particular to a perianal drug dressing of a multi-layer flow guide composite fabric and a preparation method and application of the perianal drug dressing of the multi-layer flow guide composite fabric. The perianal drug dressing is of a four-layer structure, a wet tissue adhesion layer is made of polydopamine modified chitosan / oxidized hyaluronic acid hydrogel, and wet-state adhesion is achieved; the drug sustained-release layer is used for dispersing mesoporous silica loaded with ciprofloxacin hydrochloride / defensin and chitosan microspheres loaded with epidermal growth factors / lonicera japonica into a polycaprolactone fiber membrane to achieve synergistic antibacterial and healing promotion; the directional flow guide layer is of a gradient wettability sandwich structure with a bionic micro-channel, and one-way rapid drainage can be achieved; the high-absorption outer layer is carboxymethyl cellulose / sodium polyacrylate composite sponge, liquid is locked efficiently, the wound surface is kept dry and comfortable, the clinical problem in perianal infection wound treatment can be effectively solved, and the treatment effect and the patient comfort are remarkably improved.

The invention discloses a culture system, culture method and application of gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system comprises: a basic culture medium, which is a DMEM / F12 culture medium, and is additionally added with a special growth promoting factor obtained from a natural extract; the multiple growth factors comprise an epidermal growth factor (EGF), a fibroblast growth factor (FGF), a bone morphogenetic protein (BMP) and a vascular endothelial growth factor receptor inhibitor (VEGFRi), and the concentration of each growth factor is accurately screened and optimized; the extracellular matrix is Matrigel modified by physical and chemical methods; the specific additive comprises an antioxidant N-acetylcysteine (NAC). According to the culture system, the culture method and the application of the gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system which is closer to a real environment in vivo is constructed by optimizing basic culture medium components, setting various growth factors and specific additive concentrations and adopting modified Matrigel as an extracellular matrix.

The invention discloses a pseudo-ginseng exosome-like nano vesicle loaded with an epidermal growth factor / application of the pseudo-ginseng exosome-like nano vesicle in wound healing. The research shows that not all exosome-like nano-vesicles from Chinese herbal medicines capable of promoting wound healing have the effect of promoting wound healing, the action mechanism of the pseudo-ginseng exosome-like nano-vesicles in wound healing is further explored, and the research shows that PNVs can promote the proliferation and migration of an epidermal keratinocyte line, so that the PNVs can be used for promoting the healing of the wound. The treatment can be realized by regulating and controlling the activity of immune cells to promote cell proliferation and migration, and the wound healing effect of the EGF-loaded EGF-coated PNVs is remarkably enhanced, so that the EGF-loaded EGF-coated PNVs have a synergistic effect. The invention not only provides a theoretical basis for deeply researching PELNVs, but also provides a new method and a new idea for treating acute wounds.

The invention provides hydrogel with a wound healing function and a preparation method thereof.The hydrogel is prepared from, by mass, 50-70 parts of basic gel matrix, 20-30 parts of natural medicine extract and 8-10 parts of bioactive ingredients; the natural medicine extract comprises superfine pearl powder, a semen cassiae extract, an aloe extract, a cortex phellodendri extract, a honeysuckle flower extract, a dandelion extract, a centipede extract, a fructus forsythiae extract and a green tea extract; the bioactive components comprise epidermal growth factors, heparin sodium, mussel mucin and nano-silver ions; on the basis of a commercially available compound cortex phellodendri liniment, further research and development are carried out, the superfine pearl powder, the semen cassiae extract, the aloe extract, the green tea extract and other natural medicine components are added, the optimal proportion is selected, and the basic gel matrix, the bioactive components and the like are combined, so that the wound healing rate, the antibacterial rate and the water holding rate of the hydrogel are remarkably improved finally.

The invention discloses a composite gel for skin correction and a preparation method thereof. The composite gel is formed by dissolving sodium hyaluronate, epidermal growth factors and a protein stabilizer in a buffer system; the content of the sodium hyaluronate is 1.0-50.0 g / L, the content of the epidermal growth factor is 1000-30000 IU / mL, and the content of the stabilizer is 5.0-100.0 mg / L; the pH value of the buffer system is 6.5-7.5. The preparation method comprises the following steps: S1, dissolving sodium hyaluronate and a stabilizer in a buffer system under a sterile condition, and stirring to obtain gel A; s2, adding epidermal growth factors into the gel A, and stirring to obtain the composite gel. The composite gel disclosed by the invention can activate epithelial fibroblasts, update and reconstruct ECM and maintain the stability of an ECM microenvironment. The composite gel disclosed by the invention integrates the functions of supplementing lost extracellular matrix, increasing the number of cells and promoting synthesis of collagen by a traditional filling material, can solve the skin problems caused by various factors, and achieves the effect of correcting the skin.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The invention discloses a lung airway organoid culture system with a trachea branch morphological structure as well as application and a culture method of the lung airway organoid culture system, and relates to the technical field of lung airway organoid culture. The hydrogel culture system is prepared from I-type rat tail collagen, gelatin, hyaluronic acid, laminin, low-melting agarose, chondroitin sulfate, fibronectin, elastin, epidermal growth factors, fibronectin L and transglutaminase; the induction culture medium is prepared from a 1XB27 supplement, a fiber growth factor, glutamine, glutathione, A8301, SB202190, IBMX, 8-Br-cAMP, FGF7, FGF10, Shh and ascorbic acid. The culture system is clear in component, low in price and cost and simple and convenient in preparation method, more importantly, the lung airway organoid which is closer to the physiological state can be efficiently induced to be formed within a short time, and the generated organoid has a branch form similar to an in-vivo trachea and contains various types of functional airway epithelial cells at the same time; and a more preferable scheme is provided for constructing an airway organ with an airway branch form based on the adult lung stem cells.

The application provides a culture medium and a culture method of ovarian cancer primary cells. The culture medium comprises an MST1 / 2 kinase inhibitor, sodium pyruvate, forskolin, epidermal growth factor, gastrin, fibroblast growth factor 7, nicotinamide, SB431542, and fetal bovine serum. Compared with the existing culture method, the in-vitro culture using the culture medium of the application has higher amplification efficiency; the culture of the ovarian cancer primary cells using the culture medium can maintain the morphological structure and pathological characteristics of the primary tissue, and improve the success rate and survival rate of the ovarian cancer primary cell culture.

A novel fused pyrimidine based sulfonamide derivative represented by Formula I, a solvate thereof, or a pharmaceutically acceptable salt thereof, a pharmaceutical composition including the same as an active ingredient, and the uses thereof are disclosed. The novel fused pyrimidine based sulfonamide derivative can effectively suppress the growth of cancer cells and resistance to drugs, which are induced by mutations in the tyrosine kinase domain of epidermal growth factor receptors, or the growth of cancer cells having such resistance.

Provided are: a culture medium which is for producing an organoid and which comprises a prostaglandin, hepatocyte growth factor (HGF), an interleukin (IL)-6 family protein, and an epidermal growth factor (EGF) family protein; a kit for cultivating an organoid; a method for producing an organoid; and an organoid.

The application relates to the technical field of medical dressing preparation, and discloses a hemostatic, healing-promoting and antibacterial foam dressing and a preparation method thereof. The method uses sodium alginate and carboxymethyl chitosan as a composite polysaccharide matrix, mixes the two, adds a calcium chloride solution to crosslink a porous gel support, and then carries out freeze-drying to obtain a porous framework; gelatin and polyvinyl alcohol can be further added to enhance the mechanical strength, zeolite particles loaded with silver ions are added to achieve antibacterial performance, epidermal growth factor release microspheres are added to promote healing, and the dressing can be further subjected to heparinization, plasma surface modification, thrombin addition, salt precipitation pore formation and sterilization treatment. The dressing prepared by the method has the functions of synergistic hemostasis, antibiosis and healing promotion, has good biocompatibility and suitable mechanical strength, and has a simple preparation process, and can be used for various wound care.

The invention belongs to the technical field of exosomes, and discloses an exosome preparation method and application, the exosome preparation method comprises the following steps: S1, culturing target cells in an induction medium, and collecting the cells and a culture solution; s2, separating the culture solution to obtain supernate A and cell precipitate; s3, performing chromatographic purification and concentration on the supernate A to obtain the exosome; in the step S1, colchicine is also added in the process of culturing the target cells to synchronize the cell cycle; a polycaprolactone-gelatin composite microcarrier is suspended in the induction culture medium, and nicotinamide, an epidermal growth factor (EGF) and pyruvic acid are contained in the induction culture medium; the method further comprises a step S30 before the step S3, resuspending the cell precipitate obtained in the step S2 with a vesicle protection solution containing trehalose, sucrose and EDTA to obtain a supernatant B, and then combining the supernatant A with the supernatant B. According to the preparation method provided by the invention, the total secretion amount of target cells is large, the intracellular vesicle-exosome precursor can be collected, and the obtained exosome is high in concentration and low in impurity content.

The invention discloses a system for directionally differentiating chicken primordial germ cells into gametes in vitro and a regulation and control method, the system comprises a basic culture medium: based on DMEM / F12, adding 10% of fetal calf serum, 2% of chicken serum, 2 mmol / L of L-glutamine, 1 mmol / L of sodium pyruvate and 5.5 * 10 <-5 > mol / L of beta-mercaptoethanol; the spermatogonium induction culture medium further comprises BMP4, RA and GDNF; the oogonium induction culture medium also comprises BMP15, GDF9 and EGF (Epidermal Growth Factor). According to the system and the regulation and control method, the differentiation efficiency is improved, the culture period is shortened, and the number of cell clusters is remarkably increased.

Substituted pyrrolo[2,3-d]pyrimidines as inhibitors for multi-resistant cancers are described herein. Also provided herein are methods of forming the compounds, methods for inhibiting aurora kinase A and / or aurora kinase B activity and epidermal growth factor activity, methods of treating, ameliorating, or preventing cancer, and uses of the compounds for inhibiting aurora kinase A and / or aurora kinase B activity and epidermal growth factor receptor kinase activity. The compounds are generally of the formulas as illustrated herein, including those of Formula (I-2) or a pharmaceutically acceptable salt thereof:wherein: X1 is N; X2 is N; X3 is —NH—; X4 is N or CR2; X5 is CH, CCH3, or CCOOH; L1 is —NR4—R1; L2 is NH2 or H; R1 is:R2 is H, COOH, a 5-membered heteroaryl, or X6R3; X6R3 is as described herein; R4 is H; R5 is as described herein; and m is 1, 2, 3, 4, or 5.

The present invention relates to human monoclonal antibodies that bind to epidermal growth factor-like repeats and discoidin I-like domain 3 (EDIL3) and methods of use thereof.

This invention relates to a skin cell dispersion for autologous treatment of skin wounds. Specifically, this invention provides a skin cell dispersion containing skin cells, epidermal growth factor, and transforming growth factor-β1. The skin cell dispersion of this invention exhibits excellent therapeutic effects on skin wounds.

The present disclosure relates to a composition comprising a peptide having at least 90% sequence homology to a peptide of SEQ ID No. 1 and epidermal growth factor (EGF). It also relates to a process of preparing the composition and its use in treating wounds, including diabetic wound complications.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

The invention provides an intrahepatic cholangiocarcinoma organ culture medium and a culture method. The culture medium is prepared from the following components according to final concentration: 0.5 to 200 mu M of A83-01, 0.1 to 100 mu M of EGF (Epidermal Growth Factor), 10 to 500 ng / ml of FGF10 (Fibroblast Growth Factor), 1 to 200 ng / ml of HGF (Hepatitis Growth Factor), 0.5 to 200 mu M of Forskolin, 0.01 to 20 mu M of Gastrin I, 0.5 to 100 mM of Nicotinamide, 1 to 500 mu M of Y-27632, 0.5 to 100 nM of dexamethasone and 20 to 500 mu g / ml of primary cell antibiotics. All the components are dissolved in a DMEM / F12 culture solution. When the culture medium is used for culturing the intrahepatic cholangiocellular carcinoma organoid, the rapid amplification of the intrahepatic cholangiocellular carcinoma stem cells and the maturation and differentiation of the organoid are facilitated, the passage times are obviously increased, and the same tissue structure and genome characteristics of primary tissues can still be maintained after multiple passages.

The invention provides an application of an epidermal growth factor (EGF) in induction of apoptosis of an upper urinary tract epithelial carcinoma organ (UTUC), and belongs to the technical field of biomedicine. The invention relates to an application of an EGF (Epidermal Growth Factor) or EGFR (Epidermal Growth Factor Receptor) agonist in inducing The EGF induces apoptosis of the upper urinary tract epithelial cancer organoid by activating the STAT1-Caspase 3 pathway and up-regulating the expression level of the EGFR. In view of the fact that the upper urinary tract epithelium carcinoma organoid and the upper urinary tract epithelium carcinoma tissue are highly consistent in form and regulation mechanism, the technical scheme provided by the invention provides a new thought for the medicine for preventing and / or treating the upper urinary tract epithelium carcinoma.

The present invention provides a special culture medium for breast cancer organoids and a culture method thereof, belonging to the technical field of primary cell culture. A special culture medium for breast cancer organoids is composed of a basal medium and culture additives. The basal medium is DMEM / F12 medium, and the culture additives are composed of epidermal growth factor, basic fibroblast growth factor, keratinocyte growth factor, fibroblast growth factor 10, insulin, transferrin, sodium selenite, A83-01, SB202190, insulin-like growth factor 1, B27, nicotinamide and N-acetylcysteine. The culture medium of the present invention improves the stability of the tumor cell genome during the culture while reducing the culture cost.

The invention discloses an internal jugular vein semi-permanent catheter with a self-adaptive epidermal growth factor release mechanism, and belongs to the technical field of medical instruments. The catheter comprises a catheter body and a multifunctional medicine release covering film arranged at the telecentric end in a covering mode, the covering film is made of biocompatible polymers, epidermal growth factors (EGF) and antibacterial agents are loaded on the covering film in a gradient mode, and a micro sensor is arranged in the covering film. The sensor is linked with an intelligent feedback system, the drug release rate can be dynamically adjusted according to local physiological conditions, and meanwhile wireless data transmission and remote monitoring are supported. The defects of an existing semi-permanent catheter in the aspects of infection prevention, healing promotion and dynamic adjustment are overcome, targeted drug release, continuous antibiosis, self-adaptive feedback and remote management are achieved, and the long-term implantation requirement is met.

The invention relates to a culture method and a culture medium for reducing the number of differential transcripts of epithelial organoid and in-vivo tissues, and solves the technical problems of low growth rate and low formation rate of the organoid in the prior art. The basic components of the culture medium comprise a DMEM / F12 basal culture medium, a penicillin-streptomycin solution, a Glutamax additive, a recombinant spinal protein, a recombinant head protein, Y-27632, an epidermal growth factor, an N-2-hydroxyethyl piperazine-N-2-ethanesulfonic acid buffer solution, nicotinamide, A83-01 and CHIR99021, and the additional components of the culture medium comprise a Wnt-3A additive, an ITS-X additive and hydrocortisone. The invention also provides a culture method of the epithelial organoid. The method can be used for culturing epithelial organs.

In one aspect, the present disclosure relates to lipid nanoparticles (LNPs) comprising at least one ionizable lipid, at least one helper lipid, cholesterol, and at least one polymer conjugated lipid. In certain embodiments, the LNP further comprises at least one cargo molecule. In certain embodiments, the LNP comprises an epidermal growth factor (EGFR) targeting domain. In another aspect, the present disclosure provides a method of delivering a cargo to the placenta of a subject. In another aspect, the present disclosure provides a method of treating, preventing, and / or ameliorating a placental disease and / or disorder in a subject. In certain embodiments, the placental disease and / or disorder is pre-eclampsia.

The invention relates to the technical field of biological medicines, and particularly discloses an application composition of stem cell exosomes in promoting tissue repair, which comprises the stem cell exosomes selected from induced pluripotent stem cells, mesenchymal stem cells, embryonic stem cells, hematopoietic stem cells or neural stem cells and used for promoting tissue repair and cell regeneration, and the stem cell exosomes selected from the induced pluripotent stem cells, the mesenchymal stem cells, the embryonic stem cells, the hematopoietic stem cells and the neural stem cells. The tissue repair promoting factor is selected from an epidermal growth factor, a transforming growth factor beta, a platelet-derived growth factor or a combination thereof, and is used for optimizing cell proliferation, differentiation and wound healing; the biodegradable carrier is selected from hydrogel, lipidosome, polymer micelle, nano particles or natural plant extract; by optimizing the transmission relation between the biodegradable carrier and the stem cell exosome and combining with the cell proliferation model, different doses and delivery modes can be flexibly regulated and controlled, the treatment scheme is further optimized, and the effect of the treatment scheme is accurately predicted. Due to the design, the common problems of unstable effect, long treatment period and the like in the traditional stem cell application are solved.

The present invention relates to an epithelial organoid culture medium and a culture method, which solve the technical problems of low growth rate and low formation rate of organoids in the prior art. The epithelial organoid culture medium contains basic components and additional components. The basic components include DMEM / F12 basal medium, penicillin-streptomycin solution, Glutamax additive, recombinant spondin, recombinant norrin, Y-27632, epidermal growth factor, N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid buffer solution, nicotinamide, A83-01 and CHIR99021. The additional components include Wnt-3A, ITS-X additive and hydrocortisone. The present invention also provides a culture method for epithelial organoids. The present invention can be used for the culture of epithelial organoids.

The application belongs to the field of fluorescent probes, and particularly relates to a tumor-targeting photothermal reagent based on fluoro-rhodamine, and a preparation method and application thereof. To solve the problems that most of existing photothermal reagents have difficulty in post-modification due to complex synthesis, poor biological compatibility due to large molecular weight, and lack of tumor targeting of these molecules, and generally need to be made into nanoparticles to enrich to the tumor site by EPR effect, the application develops a tumor-targeting photothermal reagent Tag-880-NHS based on fluoro-rhodamine, which has the characteristics of simple synthesis, good water solubility, good light stability, high photothermal conversion efficiency, and an absorption wavelength located in the NIR region, and can be used for labeling a specific targeting epidermal growth factor monoclonal antibody-panitumumab, and can be used in the fields of tumor imaging and treatment, and has important application value.

The invention relates to the technical field of growth factor monitoring systems, and particularly discloses an intelligent optimization method and system for an animal-derived epidermal growth factor. The method mainly comprises the following steps: determining a target strain for efficiently expressing the epidermal growth factor, wherein the strain is a genetic engineering strain containing the animal-derived epidermal growth factor; determining culture conditions of the target strain; monitoring the physiological indexes of the target strain; determining the current growth stage of the target strain according to the physiological indexes; and optimizing the culture conditions of the target strain according to the current growth stage of the target strain. The problem that the yield and expression efficiency of the epidermal factor are limited due to the fact that specific requirements of the strain in different growth stages are difficult to meet when the genetic engineering strain is cultured under fixed culture conditions in the prior art is effectively solved, and the yield and expression efficiency of the epidermal factor are improved by identifying and utilizing the growth cycle of the strain and dynamically adjusting the culture conditions. The specific requirements of the strain in different growth stages are met, so that the yield and the expression efficiency of the epidermal factor are remarkably improved.

The present invention relates to the technical field of bedsores, and in particular to the application of ESC-Exos introduced with Ca-I-Ti and cross-linked with EGF in the treatment of bedsores. This application is to introduce Ca-I-Ti cross-linked epidermal growth factor by electroporation of embryonic stem cell exosomes, and administer the drug through hydrogel microneedles to treat wound recovery and bacterial infection of bedsores. The application of ESC-Exos introduced with Ca-I-Ti and cross-linked with EGF in the treatment of bedsores provided by the present invention is to bring ESC-Exos introduced with Ca-I-Ti and cross-linked epidermal growth factor to the deep part of necrotic tissue through nano-microneedles, and perform antibacterial treatment of bedsores through the bactericidal effect achieved by Ca-I-Ti to avoid antibiotic resistance, and then enter the necrotic tissue cells through ESC-Exos for intracellular repair, and promote vascular growth and tissue repair of necrotic tissue through epidermal growth factor, and finally perform long-term slow release through gel permeation administration to complete local repair.