294 results about "Epidermal growth factor" patented technology

The invention relates to the field of cell biology, and particularly discloses an exosome as well as a preparation method and application thereof. The method for preparing the exosome comprises the following steps: (1) introducing nucleotide sequences for coding a signal peptide TPA and an epidermal growth factor into an AAVS1 region of a host cell, such as a sequence shown in SEQ ID No.9 and a sequence shown in SEQ ID No.10, so as to obtain a modified host cell; and (2) collecting the exosome secreted by the modified host cell. The expression level of EGF mRNA in the exosome can be remarkably improved, so that the obtained exosome has a better effect in skin wound treatment.

The invention relates to the technical field of medical dressings, in particular to a perianal drug dressing of a multi-layer flow guide composite fabric and a preparation method and application of the perianal drug dressing of the multi-layer flow guide composite fabric. The perianal drug dressing is of a four-layer structure, a wet tissue adhesion layer is made of polydopamine modified chitosan / oxidized hyaluronic acid hydrogel, and wet-state adhesion is achieved; the drug sustained-release layer is used for dispersing mesoporous silica loaded with ciprofloxacin hydrochloride / defensin and chitosan microspheres loaded with epidermal growth factors / lonicera japonica into a polycaprolactone fiber membrane to achieve synergistic antibacterial and healing promotion; the directional flow guide layer is of a gradient wettability sandwich structure with a bionic micro-channel, and one-way rapid drainage can be achieved; the high-absorption outer layer is carboxymethyl cellulose / sodium polyacrylate composite sponge, liquid is locked efficiently, the wound surface is kept dry and comfortable, the clinical problem in perianal infection wound treatment can be effectively solved, and the treatment effect and the patient comfort are remarkably improved.

The invention relates to a culture solution for recovering the quality of porcine oocytes by adding uridine. The culture solution comprises a basic culture solution and uridine, the basic culture solution contains 10% of pig follicular fluid, 0.57 mM of cysteine, 0.5 IU / mL of LH, 0.5 IU / mL of FSH, 10 ng / mL of epidermal growth factors, 1% of a double-antibody TCM 199 culture medium and the balance of water; the addition amount of uridine is 100 [mu] M of the basic culture solution. According to the method disclosed by the invention, metabolite uridine (Urd) required by a human body is used for respectively rescuing in-vitro cultured H2O2 induced oxidative stress porcine oocytes and delayed cultured aged porcine oocytes, so that the quality of the oocytes is improved.

The invention discloses a serum-free culture medium for in-vitro proliferation of poultry muscle stem cells and application of the serum-free culture medium, and belongs to the technical field of cell engineering, a basic serum-free culture medium is DMEM-F12, a complete serum culture medium is a mixture of DMEM-F12 added with 10% of fetal calf serum and 1% of mycillin, and the volume ratio of a culture medium supplement to the basic culture medium is 1: 1000; the serum-free culture medium contains a supplement; the supplement comprises the following compositions: insulin, transferrin, fibroblast growth factor 2, epidermal growth factor, catalase, ethanolamine, vitamin B12, linoleic acid, linolenic acid, retinol acetate, tocopherol, L-alanine, L-proline, sodium selenite, sodium pyruvate, reduced glutathione, berberine hydrochloride and analogues thereof.

The invention relates to the technical field of bioengineering, in particular to recombinant saccharomyces cerevisiae for producing a human epidermal growth factor based on a biological membrane as well as a construction method and application of the recombinant saccharomyces cerevisiae. According to the invention, saccharomyces cerevisiae BY4742 and 530-1C are used as chassis cells for expressing the human epidermal growth factor (hEGF), and the yield of the human epidermal growth factor (hEGF) is increased to 15 mg / L from 80 [mu] g / L by means of gene modification, promoter optimization, signal peptide optimization and the like; by optimizing related modules in an engineering protein secretion pathway, single or combined deletion of degrading enzyme genes (PEP4, PRB1 and YAP3) and overexpression of key node genes (KAR2, PDI1, HAC1, ERV29, ERO1, SRP54, SEC24 and DER1) in the protein secretion pathway, the hEGF yield is increased to the level of 141 mg / L; a biological membrane strain with the performance of flocculation and adhesion to an abiotic surface is constructed by selecting an endogenous flocculation gene FLO11 of overexpression yeast and an exogenous gene ALS3 of tritirachium album, and continuous production of hEGF is realized based on biological membrane immobilized continuous fermentation, and the yield is stabilized at 300 mg / L or above.

The invention provides a skin care composition containing stem cell extract as well as a preparation method and application of the skin care composition, and belongs to the technical field of cosmetics. The method comprises the following steps: mixing and culturing the extracted urine-derived stem cells and pilose antler stem cells in a DMEM culture medium added with epidermal growth factors, cholera toxin, penicillin, streptomycin, transferrin, IL-2 and IL-15, then separating a culture solution to obtain mixed exosomes, mixed stem cells and extracellular matrixes, preparing the mixed stem cells into stem cell lipidosome, and preparing the stem cell lipidosome. Embedding the stem cell extract and the mixed exosome into titanium dioxide / silicon dioxide microspheres, adding into an extracellular matrix, and uniformly dispersing to obtain the skin care composition containing the stem cell extract. The skin care composition containing the stem cell extract prepared by the invention can obviously prolong the preservation period of stem cells and exosomes and improve the repair and improvement of the stem cells and the exosomes on skin, and the prepared composition has better effects of resisting aging, whitening, promoting skin elasticity, resisting wrinkles and the like, and has a wide application prospect.

The invention discloses a culture system, culture method and application of gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system comprises: a basic culture medium, which is a DMEM / F12 culture medium, and is additionally added with a special growth promoting factor obtained from a natural extract; the multiple growth factors comprise an epidermal growth factor (EGF), a fibroblast growth factor (FGF), a bone morphogenetic protein (BMP) and a vascular endothelial growth factor receptor inhibitor (VEGFRi), and the concentration of each growth factor is accurately screened and optimized; the extracellular matrix is Matrigel modified by physical and chemical methods; the specific additive comprises an antioxidant N-acetylcysteine (NAC). According to the culture system, the culture method and the application of the gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system which is closer to a real environment in vivo is constructed by optimizing basic culture medium components, setting various growth factors and specific additive concentrations and adopting modified Matrigel as an extracellular matrix.

The invention discloses a pseudo-ginseng exosome-like nano vesicle loaded with an epidermal growth factor / application of the pseudo-ginseng exosome-like nano vesicle in wound healing. The research shows that not all exosome-like nano-vesicles from Chinese herbal medicines capable of promoting wound healing have the effect of promoting wound healing, the action mechanism of the pseudo-ginseng exosome-like nano-vesicles in wound healing is further explored, and the research shows that PNVs can promote the proliferation and migration of an epidermal keratinocyte line, so that the PNVs can be used for promoting the healing of the wound. The treatment can be realized by regulating and controlling the activity of immune cells to promote cell proliferation and migration, and the wound healing effect of the EGF-loaded EGF-coated PNVs is remarkably enhanced, so that the EGF-loaded EGF-coated PNVs have a synergistic effect. The invention not only provides a theoretical basis for deeply researching PELNVs, but also provides a new method and a new idea for treating acute wounds.

The invention provides hydrogel with a wound healing function and a preparation method thereof.The hydrogel is prepared from, by mass, 50-70 parts of basic gel matrix, 20-30 parts of natural medicine extract and 8-10 parts of bioactive ingredients; the natural medicine extract comprises superfine pearl powder, a semen cassiae extract, an aloe extract, a cortex phellodendri extract, a honeysuckle flower extract, a dandelion extract, a centipede extract, a fructus forsythiae extract and a green tea extract; the bioactive components comprise epidermal growth factors, heparin sodium, mussel mucin and nano-silver ions; on the basis of a commercially available compound cortex phellodendri liniment, further research and development are carried out, the superfine pearl powder, the semen cassiae extract, the aloe extract, the green tea extract and other natural medicine components are added, the optimal proportion is selected, and the basic gel matrix, the bioactive components and the like are combined, so that the wound healing rate, the antibacterial rate and the water holding rate of the hydrogel are remarkably improved finally.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium which is composed of a basic culture medium and an additive, and the basic culture medium is DMEM / F12. The additive is prepared from albumin, transferrin, insulin, a fibroblast growth factor (FGF), an epidermal growth factor (EGF), dexamethasone, glutamine, ascorbic acid, recombinant human III type collagen and recombinant human XVII type collagen. According to the UCMSCs obtained by culturing the umbilical cord mesenchymal stem cells by using the serum-free culture medium, the cellular morphology and surface marker expression are maintained; the umbilical cord mesenchymal stem cell serum-free culture medium has the advantages that the cell proliferation capacity, the monoclonal performance capacity and the three-line differentiation capacity are enhanced, the umbilical cord mesenchymal stem cell serum-free culture medium only contains 12 raw materials, the compounding process is simple, the cost is low, the raw materials of the umbilical cord mesenchymal stem cell serum-free culture medium are clear in quality, and the umbilical cord mesenchymal stem cell serum-free culture medium can be used for culturing clinical-grade UCMSCs.

The invention provides a special culture medium for breast cancer organoid and a culture method thereof, and belongs to the technical field of primary cell culture. The special culture medium for the breast cancer organoid is composed of a basic culture medium and a culture additive, and the basic culture medium is a DMEM (Dulbecco Modified Eagle Medium) / F12 culture medium. The culture additive is prepared from an epidermal growth factor, a basic fibroblast growth factor, a keratinocyte growth factor, a fibroblast growth factor 10, insulin, transferrin, sodium selenite, A83-01, SB202190, an insulin-like growth factor 1, B27, nicotinamide and N-acetylcysteine. According to the culture medium, the culture cost is reduced, and meanwhile, the stability of tumor cell genomes in the culture process is improved.

The invention discloses a composite gel for skin correction and a preparation method thereof. The composite gel is formed by dissolving sodium hyaluronate, epidermal growth factors and a protein stabilizer in a buffer system; the content of the sodium hyaluronate is 1.0-50.0 g / L, the content of the epidermal growth factor is 1000-30000 IU / mL, and the content of the stabilizer is 5.0-100.0 mg / L; the pH value of the buffer system is 6.5-7.5. The preparation method comprises the following steps: S1, dissolving sodium hyaluronate and a stabilizer in a buffer system under a sterile condition, and stirring to obtain gel A; s2, adding epidermal growth factors into the gel A, and stirring to obtain the composite gel. The composite gel disclosed by the invention can activate epithelial fibroblasts, update and reconstruct ECM and maintain the stability of an ECM microenvironment. The composite gel disclosed by the invention integrates the functions of supplementing lost extracellular matrix, increasing the number of cells and promoting synthesis of collagen by a traditional filling material, can solve the skin problems caused by various factors, and achieves the effect of correcting the skin.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The invention discloses a stem cell-derived function-enhanced mesenchymal stromal cell as well as a preparation method and application thereof. The preparation method comprises the following steps: inserting an IL-10 gene coding sequence and a CD47 gene coding sequence into a human pluripotent stem cell; a neural crest cell differentiation culture medium containing a Wnt pathway agonist and an ALK5 inhibitor is used for carrying out induced differentiation culture on the CD47 positive human pluripotent stem cells to obtain the neural crest cells. The neural crest cells are subjected to induced differentiation culture by adopting a transition culture medium containing an epidermal growth factor, a fibroblast growth factor 2 and a cell apoptosis inhibitor and a mesenchymal stromal cell differentiation culture medium containing the epidermal growth factor, the fibroblast growth factor 2 and a platelet growth factor in sequence; according to the present invention, with the combination of the specific embodiments, the obtained mesenchymal stromal cell highly expresses CD47 and IL-10, the expression of the CD47 can help the transplanted cell escape from the attack of the immune system and prolong the cell survival time, and the expression of the IL-10 can be used for anti-inflammation.

The invention discloses a lung airway organoid culture system with a trachea branch morphological structure as well as application and a culture method of the lung airway organoid culture system, and relates to the technical field of lung airway organoid culture. The hydrogel culture system is prepared from I-type rat tail collagen, gelatin, hyaluronic acid, laminin, low-melting agarose, chondroitin sulfate, fibronectin, elastin, epidermal growth factors, fibronectin L and transglutaminase; the induction culture medium is prepared from a 1XB27 supplement, a fiber growth factor, glutamine, glutathione, A8301, SB202190, IBMX, 8-Br-cAMP, FGF7, FGF10, Shh and ascorbic acid. The culture system is clear in component, low in price and cost and simple and convenient in preparation method, more importantly, the lung airway organoid which is closer to the physiological state can be efficiently induced to be formed within a short time, and the generated organoid has a branch form similar to an in-vivo trachea and contains various types of functional airway epithelial cells at the same time; and a more preferable scheme is provided for constructing an airway organ with an airway branch form based on the adult lung stem cells.

The invention discloses an amniotic mesenchymal stem cell exosome and a preparation method and application thereof.The preparation method comprises the following steps that amniotic mesenchymal stem cells are inoculated into a complete culture medium to be cultured, and after the cells adhere to the wall, epidermal growth factors and polypeptide are added into the complete culture medium to continue to be cultured; replacing the complete culture medium, continuously culturing until the cell fusion degree is not lower than 85%, replacing the complete culture medium again, culturing, and collecting supernate; and extracting the exosome in the supernate by adopting an ultracentrifugation method to obtain the amniotic mesenchymal stem cell exosome. According to the preparation method of the amniotic mesenchymal stem cell exosome, the epidermal growth factor and the polypeptide are added into the culture medium, so that secretion of the cell exosome can be stimulated, meanwhile, carrying substances of the exosome are improved, the functions of promoting cell proliferation, migration and the like of the exosome are improved, and then healing of skin injury can be better promoted; the wound healing time is obviously shortened, and the pain of a patient is relieved.

The application provides a culture medium and a culture method of ovarian cancer primary cells. The culture medium comprises an MST1 / 2 kinase inhibitor, sodium pyruvate, forskolin, epidermal growth factor, gastrin, fibroblast growth factor 7, nicotinamide, SB431542, and fetal bovine serum. Compared with the existing culture method, the in-vitro culture using the culture medium of the application has higher amplification efficiency; the culture of the ovarian cancer primary cells using the culture medium can maintain the morphological structure and pathological characteristics of the primary tissue, and improve the success rate and survival rate of the ovarian cancer primary cell culture.

A novel fused pyrimidine based sulfonamide derivative represented by Formula I, a solvate thereof, or a pharmaceutically acceptable salt thereof, a pharmaceutical composition including the same as an active ingredient, and the uses thereof are disclosed. The novel fused pyrimidine based sulfonamide derivative can effectively suppress the growth of cancer cells and resistance to drugs, which are induced by mutations in the tyrosine kinase domain of epidermal growth factor receptors, or the growth of cancer cells having such resistance.

Provided are: a culture medium which is for producing an organoid and which comprises a prostaglandin, hepatocyte growth factor (HGF), an interleukin (IL)-6 family protein, and an epidermal growth factor (EGF) family protein; a kit for cultivating an organoid; a method for producing an organoid; and an organoid.

The application relates to the technical field of medical dressing preparation, and discloses a hemostatic, healing-promoting and antibacterial foam dressing and a preparation method thereof. The method uses sodium alginate and carboxymethyl chitosan as a composite polysaccharide matrix, mixes the two, adds a calcium chloride solution to crosslink a porous gel support, and then carries out freeze-drying to obtain a porous framework; gelatin and polyvinyl alcohol can be further added to enhance the mechanical strength, zeolite particles loaded with silver ions are added to achieve antibacterial performance, epidermal growth factor release microspheres are added to promote healing, and the dressing can be further subjected to heparinization, plasma surface modification, thrombin addition, salt precipitation pore formation and sterilization treatment. The dressing prepared by the method has the functions of synergistic hemostasis, antibiosis and healing promotion, has good biocompatibility and suitable mechanical strength, and has a simple preparation process, and can be used for various wound care.

The invention discloses a culture medium and a culture method of a breast cancer organoid. The culture medium comprises an Advanced DMEM / F12 basal culture medium and an additive, the additive is prepared from penicillin streptomycin / amphotericin B, a B-27 additive, L-glutamine amide, HEPES (Hydroxyethyl PES), N-Acetylcysteine, nicotinamide, SB202190, A83-01, FGF10 (Fibroblast Growth Factor 10), EGF (Epidermal Growth Factor), human Hergulin beta-1, Y27632, WNT-3A, R-spondin 1, NOGGIN (Nitrogen Growth Factor IN), FGF7 (Fibroblast Growth Factor 7) and beta-estradiol. The culture method of the breast cancer organoid comprises the step of using the breast cancer organoid culture medium, and the method specifically comprises the steps of tissue sample taking, primary tissue construction, breast cancer organoid liquid changing and breast cancer organoid subculture. According to the prepared breast cancer organoid culture medium, tumor cells taken from breast cancer tumors grow fast, the cell speed is high, and follow-up medicine screening experiments can be facilitated; after the breast cancer organoid culture medium is used for culturing the breast cancer organoid, the cellular morphology and the protein expression condition of the organoid are identified through HE and immunohistochemistry, and it can be seen that the organoid can well reduce the pathological characteristics of the tissue.

The invention belongs to the technical field of exosomes, and discloses an exosome preparation method and application, the exosome preparation method comprises the following steps: S1, culturing target cells in an induction medium, and collecting the cells and a culture solution; s2, separating the culture solution to obtain supernate A and cell precipitate; s3, performing chromatographic purification and concentration on the supernate A to obtain the exosome; in the step S1, colchicine is also added in the process of culturing the target cells to synchronize the cell cycle; a polycaprolactone-gelatin composite microcarrier is suspended in the induction culture medium, and nicotinamide, an epidermal growth factor (EGF) and pyruvic acid are contained in the induction culture medium; the method further comprises a step S30 before the step S3, resuspending the cell precipitate obtained in the step S2 with a vesicle protection solution containing trehalose, sucrose and EDTA to obtain a supernatant B, and then combining the supernatant A with the supernatant B. According to the preparation method provided by the invention, the total secretion amount of target cells is large, the intracellular vesicle-exosome precursor can be collected, and the obtained exosome is high in concentration and low in impurity content.

The invention discloses a system for directionally differentiating chicken primordial germ cells into gametes in vitro and a regulation and control method, the system comprises a basic culture medium: based on DMEM / F12, adding 10% of fetal calf serum, 2% of chicken serum, 2 mmol / L of L-glutamine, 1 mmol / L of sodium pyruvate and 5.5 * 10 <-5 > mol / L of beta-mercaptoethanol; the spermatogonium induction culture medium further comprises BMP4, RA and GDNF; the oogonium induction culture medium also comprises BMP15, GDF9 and EGF (Epidermal Growth Factor). According to the system and the regulation and control method, the differentiation efficiency is improved, the culture period is shortened, and the number of cell clusters is remarkably increased.

The invention relates to the technical field of biological medicine, and particularly discloses a culture medium formula for constructing ovarian cancer organoid and a culture method. The culture medium comprises the following components: L-glutamine 100x (L-Glutamine), L-glutamine 100x (L-Glutamine), L-glutamine 100x (L-Glutamine 4-hydroxyethylpiperazine ethanesulfonic acid (1M (hepes)); b-27 is free of a serum additive (B27); the preparation method comprises the following steps of: extracting the raw materials, namely nicotinamide; the preparation method comprises the following steps: adding N, N-acetylcysteine amide (N-Acetylcysteine); a forskolin element (Forskolin); and estradiol (beta-Estradiol), which is used for preparing the pharmaceutical composition. A TGF-[beta] inhibitor (A83-01); a p38 MAPK inhibitor (SB202190), and a p38 MAPK inhibitor, a p160ROCK small molecule inhibitor (Y27632); hepatocyte growth factor (HGF); the invention relates to a recombinant human Hergulin beta-1 protein product (Hergulin beta-1) and a preparation method of the recombinant human Hergulin beta-1 protein product. An epidermal growth factor (EGF); a fibroblast growth factor (FGF10); the invention relates to a method for preparing hydrocortisone. The method comprises the following steps: preparing hydrocortisone; and a primary cell antibiotic (Primocin). The culture solution and the culture method have the advantages that ovarian high-grade serous cancer cells can be effectively cultured and induced to develop into organoid. Noggin is not needed in the scheme, and the method is different from a traditional organoid culture method and is low in cost and simple in manufacturing process. Meanwhile, preparation of a conditioned culture medium is not needed, time can be remarkably saved, the situation that the conditioned culture medium is unstable due to too long time is avoided, and the success rate is high and is about 50%.

Substituted pyrrolo[2,3-d]pyrimidines as inhibitors for multi-resistant cancers are described herein. Also provided herein are methods of forming the compounds, methods for inhibiting aurora kinase A and / or aurora kinase B activity and epidermal growth factor activity, methods of treating, ameliorating, or preventing cancer, and uses of the compounds for inhibiting aurora kinase A and / or aurora kinase B activity and epidermal growth factor receptor kinase activity. The compounds are generally of the formulas as illustrated herein, including those of Formula (I-2) or a pharmaceutically acceptable salt thereof:wherein: X1 is N; X2 is N; X3 is —NH—; X4 is N or CR2; X5 is CH, CCH3, or CCOOH; L1 is —NR4—R1; L2 is NH2 or H; R1 is:R2 is H, COOH, a 5-membered heteroaryl, or X6R3; X6R3 is as described herein; R4 is H; R5 is as described herein; and m is 1, 2, 3, 4, or 5.

The present invention relates to human monoclonal antibodies that bind to epidermal growth factor-like repeats and discoidin I-like domain 3 (EDIL3) and methods of use thereof.

This invention relates to a skin cell dispersion for autologous treatment of skin wounds. Specifically, this invention provides a skin cell dispersion containing skin cells, epidermal growth factor, and transforming growth factor-β1. The skin cell dispersion of this invention exhibits excellent therapeutic effects on skin wounds.

The present disclosure relates to a composition comprising a peptide having at least 90% sequence homology to a peptide of SEQ ID No. 1 and epidermal growth factor (EGF). It also relates to a process of preparing the composition and its use in treating wounds, including diabetic wound complications.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

The invention provides an intrahepatic cholangiocarcinoma organ culture medium and a culture method. The culture medium is prepared from the following components according to final concentration: 0.5 to 200 mu M of A83-01, 0.1 to 100 mu M of EGF (Epidermal Growth Factor), 10 to 500 ng / ml of FGF10 (Fibroblast Growth Factor), 1 to 200 ng / ml of HGF (Hepatitis Growth Factor), 0.5 to 200 mu M of Forskolin, 0.01 to 20 mu M of Gastrin I, 0.5 to 100 mM of Nicotinamide, 1 to 500 mu M of Y-27632, 0.5 to 100 nM of dexamethasone and 20 to 500 mu g / ml of primary cell antibiotics. All the components are dissolved in a DMEM / F12 culture solution. When the culture medium is used for culturing the intrahepatic cholangiocellular carcinoma organoid, the rapid amplification of the intrahepatic cholangiocellular carcinoma stem cells and the maturation and differentiation of the organoid are facilitated, the passage times are obviously increased, and the same tissue structure and genome characteristics of primary tissues can still be maintained after multiple passages.