45 results about "Epidermal growth factor" patented technology

The invention relates to the technical field of medical dressings, in particular to a perianal drug dressing of a multi-layer flow guide composite fabric and a preparation method and application of the perianal drug dressing of the multi-layer flow guide composite fabric. The perianal drug dressing is of a four-layer structure, a wet tissue adhesion layer is made of polydopamine modified chitosan / oxidized hyaluronic acid hydrogel, and wet-state adhesion is achieved; the drug sustained-release layer is used for dispersing mesoporous silica loaded with ciprofloxacin hydrochloride / defensin and chitosan microspheres loaded with epidermal growth factors / lonicera japonica into a polycaprolactone fiber membrane to achieve synergistic antibacterial and healing promotion; the directional flow guide layer is of a gradient wettability sandwich structure with a bionic micro-channel, and one-way rapid drainage can be achieved; the high-absorption outer layer is carboxymethyl cellulose / sodium polyacrylate composite sponge, liquid is locked efficiently, the wound surface is kept dry and comfortable, the clinical problem in perianal infection wound treatment can be effectively solved, and the treatment effect and the patient comfort are remarkably improved.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The application relates to the technical field of medical dressing preparation, and discloses a hemostatic, healing-promoting and antibacterial foam dressing and a preparation method thereof. The method uses sodium alginate and carboxymethyl chitosan as a composite polysaccharide matrix, mixes the two, adds a calcium chloride solution to crosslink a porous gel support, and then carries out freeze-drying to obtain a porous framework; gelatin and polyvinyl alcohol can be further added to enhance the mechanical strength, zeolite particles loaded with silver ions are added to achieve antibacterial performance, epidermal growth factor release microspheres are added to promote healing, and the dressing can be further subjected to heparinization, plasma surface modification, thrombin addition, salt precipitation pore formation and sterilization treatment. The dressing prepared by the method has the functions of synergistic hemostasis, antibiosis and healing promotion, has good biocompatibility and suitable mechanical strength, and has a simple preparation process, and can be used for various wound care.

The invention belongs to the technical field of exosomes, and discloses an exosome preparation method and application, the exosome preparation method comprises the following steps: S1, culturing target cells in an induction medium, and collecting the cells and a culture solution; s2, separating the culture solution to obtain supernate A and cell precipitate; s3, performing chromatographic purification and concentration on the supernate A to obtain the exosome; in the step S1, colchicine is also added in the process of culturing the target cells to synchronize the cell cycle; a polycaprolactone-gelatin composite microcarrier is suspended in the induction culture medium, and nicotinamide, an epidermal growth factor (EGF) and pyruvic acid are contained in the induction culture medium; the method further comprises a step S30 before the step S3, resuspending the cell precipitate obtained in the step S2 with a vesicle protection solution containing trehalose, sucrose and EDTA to obtain a supernatant B, and then combining the supernatant A with the supernatant B. According to the preparation method provided by the invention, the total secretion amount of target cells is large, the intracellular vesicle-exosome precursor can be collected, and the obtained exosome is high in concentration and low in impurity content.

This invention relates to a skin cell dispersion for autologous treatment of skin wounds. Specifically, this invention provides a skin cell dispersion containing skin cells, epidermal growth factor, and transforming growth factor-β1. The skin cell dispersion of this invention exhibits excellent therapeutic effects on skin wounds.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

The application discloses a kind of modular serum-free cell culture medium and its application, belong to biomedical technology field.The culture medium includes: epidermal growth factor, recombinant insulin, cholesterol-phospholipid nanoparticles, beta-mercaptoethanol, pluronic F-68, glucose / glutamine, trace element mixed solution.The modular serum-free cell culture medium provided by the application can not only meet the large-scale culture of stem cells, but also be used for the industrial production of in vitro diagnostic reagent raw materials such as recombinant antigen, viral vector, recombinant protein and monoclonal antibody, with wide cell adaptability, low production cost, significant economy, stable process, and strict requirements of FDA / EMA for biological products.

The invention discloses a serum protein marker combination for detecting cerebral microhemorrhage, and belongs to the field of biological medicine detection.The serum protein marker combination comprises four serum proteins including matrix metalloproteinase 3, fibronectin-like extracellular matrix protein 1 containing epidermal growth factors, a metalloproteinase inhibiting factor 1 and uromodulin; through efficient and accurate serum protein marker combination, the defects that brain function magnetic resonance SWI examination is high in cost and long in time consumption, operation depends on high-level imaging physicians and the like are overcome, an innovative tool is provided for rapid, accurate and non-invasive screening and auxiliary diagnosis of cerebral microhemorrhage, and wide clinical application prospects are achieved.

The present application relates to compositions and methods for treating and preventing fibrosis and other diseases comprising polypeptides derived from MFG-E8, wherein the polypeptides comprise an epidermal growth factor (EGF)-like domain, a C1 domain, and optionally a signal peptide, wherein the polypeptides: (a) lack a functional C2 domain; and / or (b) lack a medin polypeptide or fragment thereof.

The present invention relates to the fields of Biotechnology and Medicine. It describes the use of therapeutic compositions comprising a compound that blocks the epidermal growth factor and an antibody that blocks the PD-1 / PD-1 ligand signalling pathway in the treatment of tumours of epithelial origin, particularly those expressing the native form of the human KRAS protein. An increase in survival was observed in patients with cancer of epithelial origin expressing native KRAS treated with said therapeutic compositions.

The invention discloses a skincare stem cell exosome separation and extraction method, and relates to the technical field of biological medicine, the skincare stem cell exosome separation and extraction method comprises the following steps: S1, culture of mixed stem cells: unfreezing urine-derived stem cells and pilose antler stem cells, resuspending in an improved DMEM culture medium, and carrying out mixed culture to prepare a mixed culture solution; epidermal growth factors, cholera toxin, penicillin, streptomycin, transferrin, IL-2 and IL-15 are added into the improved DMEM culture medium; s2, pretreatment of the mixed culture solution: performing centrifugal treatment on the mixed culture solution, and separating to obtain a precipitate and a supernatant, the precipitate being mixed stem cells. The urine-derived stem cells and the pilose antler stem cells are subjected to mixed culture and matched with the improved culture medium containing IL-2, IL-15 and other components, so that the yield and biological activity of the exosome are remarkably improved, high purity and low impurity protein residue of the exosome are achieved by means of a complete purification process of multi-step centrifugation, ultrafiltration concentration and filter membrane filtration, and the yield of the exosome is improved. The skin allergy stimulation risk is effectively reduced.

The invention relates to the technical field of protein purification, and provides a method for eukaryotic purification of alpha-syn A53T protein through HEK293 cells. The method comprises the following steps: constructing a recombinant expression plasmid containing an SNCA-A53T mutation target fragment, inserting an affinity tag, transferring into escherichia coli, and performing amplification expression to obtain an endotoxin-free superhelix plasmid for transfection; inoculating HEK293 cells, and adding L-glutamine into the culture medium; the preparation method comprises the following steps: adding endotoxin-free superhelix plasmids and polyethyleneimine into HEK293 cells in sequence, uniformly mixing, standing, adding into the HEK293 cells, and supplementing feed Feed-1, L-glutamine, glucose, epidermal growth factors (EGF) and basic fibroblast growth factors (bFGF); agarose magnetic beads are specifically combined with an affinity tag, and then the magnetic beads are washed and eluted to obtain the purified alpha-syn A53T protein. A supplementary material Feed-1, an epidermal growth factor (EGF) and a basic fibroblast growth factor (bFGF) are introduced in an induced expression stage, so that the expression quantity and the purity of the alpha-syn A53T protein are effectively improved, and the structural stability and the functional integrity of the alpha-syn A53T protein are enhanced.

The invention provides AI systems that output proposed combinations of agents such as active pharmaceutical ingredients and other agents useful for a therapeutic objectives. Systems of the invention propose cocktails of those agents and optionally simulate or model effects of those agents to evaluate the potential effects of those agents in accomplishing the therapeutic objective. Specific embodiments provide a skin care composition from the AI system. The composition includes (i) a mixture of agents that includes epidermal growth factor (EGF), fibroblast growth factor (FGF), and insulin-like growth factor (IGF-1) and one or more of: a collagen-stimulating peptide, a booster of nicotinamide phospho-ribosyl-transferase (NAMPT) expression, coenzyme Q10, a nucleic acid repair enzyme, and a ceramide; (ii) one or more liposomes encapsulating at least a portion of the mixture of agents; and (iii) a moisturizer carrying the liposomes and the mixture of agents.

The present invention relates to an epidermal growth factor derivative, referred to as tEGFha, comprising or consisting of the amino acid sequence: NSDSECPLSHDGYCLHDGVCYYIENLDRYSCNCVVGYIGYRCQYR. The present invention relates to the epidermal growth factor derivative, referred to as tEGFha, for use in the prevention and / or treatment of a disease related to increased EGFR receptor activity, such as cancer.

The invention relates to the technical field of biological medicines, and provides a high VEGF protein / miR-210 extracellular vesicle induced by a serum-free combined culture medium and application of the high VEGF protein / miR-210 extracellular vesicle. According to the invention, high VEGF protein / miR-210 extracellular vesicles are obtained based on induction of a serum-free culture medium; the serum-free culture medium comprises a basic culture medium component combination: sugar-free RPMI1640 and MCDB131 in a volume ratio of 4: 1; the metabolic regulation additive is prepared by synergistically adding sodium pyruvate, cysteine and a proline hydroxylase inhibitor and taking alanine glutamine as an energy substitute; the growth factor is a combination of polyvinyl alcohol, 4-hydroxyethylpiperazine ethanesulfonic acid, ITS-X, epidermal growth factors and basic fibroblast growth factors, wherein the ITS-X, the epidermal growth factors and the basic fibroblast growth factors are added before use. CD63 and CD81 are specifically expressed on the surface of the prepared high VEGF protein / miR-210 extracellular vesicle, the high VEGF protein / miR-210 extracellular vesicle is rich in VEGF protein and miR-210, and the high VEGF protein / miR-210 extracellular vesicle is applied to targeted therapy of allergic rhinitis by inhibiting Th2 type immunoreaction.

The invention relates to a liver cancer organoid culture medium and application thereof, and belongs to the technical field of medicines. The liver cancer organ-like culture medium disclosed by the invention is prepared from the following components: AdvDMEM F12, HEPES, GlutaMAX, B27, EGF (Epidermal Growth Factor), N-acetyl-L-cysteine, gastrin, HGF (Hepatocyte Growth Factor), FGF-10 (Fibroblast Growth Factor), A83-01, nicotinamide, N2, an adenylate cyclase activator, a three-antibody antibiotic, R-spondin1, Wnt-3a, Y-2763, Noggin, oncostatin M and FGF-23. The culture medium is rich in nutrition and reasonable in component; the liver cancer organoid cell spheres cultured by using the culture medium can stably grow for a long time, continuously amplify and repeatedly cryopreserve. When the culture medium is used for preparing the liver cancer organ model, a foundation is laid for knowing the pathogenesis of liver cancer and screening drugs.

The application belongs to the field of peptides, and discloses a polypeptide derived from lactoferrin and application thereof. The application discloses a polypeptide derived from human lactoferrin, and the amino acid sequence of the polypeptide is shown as SEQ ID NO. 1. The polypeptide can promote the growth of epidermal growth factor (EGF) and has a repairing effect. The polypeptide has an inhibitory effect on the growth of gram-negative bacteria and gram-positive bacteria represented by escherichia coli and staphylococcus aureus. The polypeptide has a good application prospect as a drug for preventing and / or reducing infectious diseases caused by microorganisms, and / or as an additive of food, health products and cosmetics.

This invention belongs to the medical field and provides a calcium carbonate manganese nanoprobe for in situ imaging detection of vascular epidermal growth factor, its preparation method, and its application. In this strategy, a DNA strand (P1 and P2) containing a protein recognition antibody, a protein recognition sequence, and a deoxyribonuclease, along with a molecular beacon DNA strand (H1), is stirred in a DMEM cell culture medium containing calcium chloride and manganese chloride to prepare the calcium carbonate manganese nanoprobe. In acidic tumor cells, this nanoprobe releases P1, P2, H1, and manganese ions. P1 and P2 can simultaneously recognize proteins, forming a complete deoxyribonuclease center, which catalyzes the cleavage of H1 under the action of the cofactor manganese ions, generating a fluorescent signal. This strategy achieves specific in situ dynamic imaging of low-abundance proteins in vivo and has great potential in biomedical research.

This document relates to methods and materials involved in identifying and / or treating mammals having membranous nephropathy (e.g., membranous nephropathy with an elevated level of a neural epidermal growth factor (EGF)-like 1 (NELL-1) polypeptide in the glomerular basement membrane (GBM)). For example, methods and materials for administering one or more immunosuppressive agents (e.g., corticosteroids, cyclosporine, or a B-cell reduction or depletion agent such as Rituximab) to treat a mammal (e.g., a human) having membranous nephropathy are provided.

The invention relates to the field of biomedical materials, and discloses a bionic multilayer dressing for chronic diabetic wounds and a preparation method. The dressing adopts a sequential coaxial electrostatic spinning technology to construct a three-layer composite fiber structure: a long-term response layer (PLGA (85: 15) / EGF / anti-scar medicine), a medium-term response layer (PLGA (50: 50) / bFGF / VEGF) and a quick response layer (PVA / levofloxacin / GM6001), and after glutaraldehyde steam crosslinking and curing, a composite fiber membrane with a stable structure is formed. According to the invention, through the combination of three polymer materials with different degradation characteristics, precise time sequence controlled release of rapid release of antibiotics for resisting infection, promotion of angiogenesis through medium-term release of growth factors and promotion of epithelialization through long-term release of epidermal growth factors is realized, and the natural healing process of wounds is effectively simulated and guided; the technical problems that a traditional dressing is single in function, and drug release and healing stages are not matched are solved, and the healing quality and efficiency of diabetic chronic wounds are remarkably improved.

The invention relates to the technical field of organoid culture, and discloses an induction culture medium for culturing a pig lung organoid. Advanced DMEM / F12 is used as a basic culture solution of the culture medium; the invention relates to a basic fibroblast growth factor composition, which is prepared from the following added components with the final concentration: 1500ng / mL of R-Spondinin, 25ng / mL of basic fibroblast growth factor 7 (FGF7), 10100ng / mL of basic fibroblast growth factor, 100ng / mL of Noggin protein, 50ng / mL of epidermal growth factor, 50ng / mL of Wnt3a protein, 500nM of TGF-beta pathway inhibitor, 5mM of ROCK inhibitor, 500nM of p38MAPK inhibitor, 1X of B27 supplement, 1.25 mM of N-acetyl-L-cysteine, 10mM of nicotinamide, 1X of glutamine supplement GlutaMAX1X, 1X of HEPES buffer solution and 1X of broad-spectrum antibiotic (Primocin). According to the method disclosed by the invention, the lung organ with the complex multi-cell lineage is efficiently and stably constructed from the pig lung adult tissue by a one-step method. The obtained organoid is high in biomimetic property and good in stability, provides a tool platform for pig lung development research, disease model construction, drug toxicology testing and regenerative medicine research, and has great scientific research value and wide application prospects.

The application discloses an amplification culture medium and a culture method of human adult epidermal stem cells, and relates to the technical field of stem cell in-vitro culture. The culture medium comprises the following components: fibroblast growth factor, interleukin-6, insulin-like growth factor-1, epidermal growth factor, insulin, growth hormone, hydrocortisone, Y-27632, A83-01, Forsklin, PY60, CHIR99021, linoleic acid, linolenic acid, alanine, asparagine, glutamic acid, glycine, proline, serine, arginine, N-acetyl-L-cysteine, beta-mercaptoethanol and CaCl2. The culture medium can continuously and stably culture human adult epidermal stem cells for more than 20 generations, and a more stable long-term in-vitro amplification system of epidermal stem cells is established. After continuous subculture, the epidermal stem cells can maintain a high proliferation rate and multiple differentiation potential, and have the ability to construct multilayer skin.

A fusion protein having a triple activity and a use thereof are provided. More specifically, a fusion protein has a triple biological activity of glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP), and epidermal growth factor 21 (FGF21). It can be used for treating type 2 diabetes (T2D), obesity, and other diseases related to glucose and lipid metabolic abnormalities.

This invention discloses a highly active duck embryo liver primary cell and its low-cost preparation method and applications. The preparation method includes: obtaining and pretreatment of duck embryo liver tissue, separation by complex enzyme digestion, preparation of cell suspension, primary cell culture, and maintenance of cell viability. The core of this method lies in the gentle digestion using a complex digestion solution composed of collagenase I, trypsin, and DNase I in specific weight ratios, followed by culture in a well-defined, non-commercial growth factor-free complete culture medium. This complete culture medium contains synergistic components such as DMEM / F12, fetal bovine serum, epidermal growth factor, hepatocyte growth factor, and an insulin-transferrin-selenium mixture. The duck embryo liver primary cells prepared by this invention exhibit high yield, high viability, and excellent liver-specific function, and can maintain their activity and function well after passage and cryopreservation.

The application provides a method for long-term in-vitro culture and expansion of primary hepatocytes and application thereof. The method for in-vitro culture and expansion of primary hepatocytes comprises using a culture medium containing interleukin-22 (IL-22), combined with hepatocyte growth factor (HGF) and / or epidermal growth factor (EGF) to induce primary hepatocytes to prepare proliferative liver precursor cells. The application combines interleukin-22 (IL-22), hepatocyte growth factor (HGF) and / or epidermal growth factor (EGF) to induce in-vitro mass expansion of primary hepatocytes, and through the method, mature-like hepatocytes with a gene expression profile extremely similar to that of primary hepatocytes and perfect functions can be obtained, and the hepatocytes expanded by the method have good in-vivo therapeutic effects.

The invention discloses a human epidermal growth factor temperature-sensitive sustained-release gel as well as a preparation method and application thereof, and belongs to the technical field of biological pharmacy. The preparation method of the human epidermal growth factor temperature-sensitive sustained-release gel comprises the following steps: S1, adding poloxamer and a thickening agent into purified water, and uniformly mixing to form a gel matrix; s2, respectively preparing a human epidermal growth factor solution and a chitosan-sodium tripolyphosphate colloid, and adding the human epidermal growth factor solution into the chitosan-sodium tripolyphosphate colloid for adsorption to obtain the chitosan-sodium tripolyphosphate colloid loaded with the human epidermal growth factor; s3, adding the chitosan-sodium tripolyphosphate colloid loaded with the human epidermal growth factors, a humectant and a stabilizer into the gel matrix, and adjusting the pH value. The gel can realize stable loading and controllable slow release of the human epidermal growth factor, so that the administration frequency and repeated administration operation are reduced, and the gel has important application value in treatment of refractory wounds.

This invention discloses a polypeptide sequence derived from lactoferrin, which possesses unique novel functions and applications. Its amino acid sequence is shown in SEQ ID NO.1. This invention obtained lactoferrin peptide samples with a purity greater than 98% through solid-phase synthesis and performed functional verification experiments in cells. Experimental results show that this lactoferrin peptide not only promotes the growth of epidermal growth factor (EGF) and has a cell repair effect, but also increases the content of elastin in cells, exerting anti-wrinkle and firming effects.

The present invention relates to a transformed Pediococcus pentosaceus strain into which a gene encoding a human epidermal growth factor (EGF) protein is introduced to express and secrete an epidermal growth factor, and a skin-improving cosmetic composition comprising same. According to the present invention, whitening, wrinkle improvement, antioxidant, and skin regeneration effects superior to those of a cosmetic composition comprising commercially available isolated / purified EGF are exhibited. In addition, the present invention facilitates large-scale production of EGF and has economical production costs, thereby providing high competitiveness as a raw material for cosmetic compositions.

The invention relates to bio-ink, a 4D bioactive scaffold and a preparation method and application of the 4D bioactive scaffold, and belongs to the technical field of biology. The biological ink provided by the invention is prepared from recombinant mussel mucin, an epidermal growth factor (EGF), methacrylic anhydride gelatin, hyaluronic acid, polydopamine, N-isopropylacrylamide, trehalose, tetrabutylammonium bromide, LAP and 18-crown ether-6. The bio-ink is used for preparing a 4D bioactive scaffold, and the obtained scaffold has a good cell adhesion effect, can release active factors as required, has temperature responsiveness, can trigger deformation at the body temperature of 37 DEG C, realizes rapid repair of tissues, is good in safety, and can be applied to tissue regeneration and medical beauty repair.