3197results about "Tissue regeneration" patented technology

The invention discloses bone cement for orthopedic repair and a preparation method thereof, and relates to the technical field of bone cement. The bone cement for orthopedic repair comprises solid-phase powder and a liquid-phase component, the solid-phase powder comprises the following components in parts by weight: 55-65 parts of composite calcium phosphate, 10-15 parts of bioactive glass, 2-3 parts of a developing agent, 1-2 parts of hydroxyapatite, 8-12 parts of a modified toughening agent and 2-3 parts of nano cellulose fibers; the liquid phase component is prepared from the following components in parts by weight: 40 to 50 parts of a sodium alginate solution, 10 to 20 parts of methacrylated gelatin, 0.5 to 1 part of transglutaminase, 30 to 60 parts of a citric acid buffer solution and 1 to 2 parts of an antibacterial agent. The preparation method comprises the following steps: reacting pentaerythritol with 12-oxododecanoic acid to obtain an intermediate 1, and reacting the intermediate 1 with dopamine to prepare the modified toughening agent. The bone cement for orthopedic repair prepared by the invention has good mechanical, self-repairing and antibacterial properties.

The invention belongs to the field of biological materials, and particularly relates to recombinant V-type humanized collagen and application thereof. The invention provides a recombinant V-type humanized collagen, the amino acid sequence of the recombinant V-type humanized collagen comprises (repetitive unit) n, and the repetitive unit comprises a sequence as shown in SEQ ID NO.1; each repeating unit is directly linked and the number n of repeating units is 10. The recombinant V-type humanized collagen can comprehensively resist skin aging.

The present application relates to chondrocyte compositions and kits comprising morselized cartilage tissue particles and a biodegradable matrix. Applications and methods of using the chondrocyte composition for cartilage grafts, repairing cartilage injuries and defects, and joint repair are provided.

The invention belongs to the technical field of biomedical engineering, and particularly relates to a preparation method and application of a titanium dental implant surface coating, and the preparation method comprises the following steps: S1, pretreatment of a titanium dental implant matrix: sequentially carrying out grinding and polishing, ultrasonic cleaning and acid etching treatment; s2, preparation of a coating precursor solution: adding nano-hydroxyapatite powder, chitosan, gelatin and titanium dioxide nanoparticles into deionized water, stirring to form a suspension, then adding a silver nitrate solution, and continuously stirring to form the coating precursor solution; s3, coating a coating: coating the surface of the pretreated titanium dental implant matrix with the coating precursor solution by adopting an electrophoretic deposition method; and S4, drying and sintering: drying and sintering the coated titanium dental implant. According to the preparation method of the titanium dental implant surface coating, provided by the invention, the bonding strength between the coating and the titanium dental implant matrix can be improved.

The invention discloses a 3D intestinal organ differentiation method based on human pluripotent stem cells and an induction culture medium and application thereof, and relates to the technical field of stem cells. According to a culture medium formula combination, intestinal organs can be differentiated into various cell types such as epithelial cells, neuroendocrine cells and endothelial cells; the method is a key mark for successful differentiation and functional maturation of intestinal organs. According to the 3D intestinal organ differentiation method disclosed by the invention, histological structures such as intestinal crypts are differentiated from intestinal organs generated by differentiation, and the intestinal organs can creep in a maintenance stage, so that the intestinal organs are changed from structural bionics to functional simulation, and the significance of the 3D intestinal organ differentiation method is far better than that of pure morphological simulation. Through a systematic culture medium formula, a clear operation process and a multi-stage induction strategy, the 3D intestinal organ with structural integrity, cell diversity and functional activity is successfully constructed, and the system provides an efficient, reliable and extensible in-vitro model platform for intestinal biological research and related application.

Adhesive compositions including a multivalent metal salt, an multidentate acidic organic compound, and an aqueous medium are disclosed. The multivalent metal salt and multidentate acidic organic compound can include, as a mixture, a carbonate salt and / or carbonic acid. The aqueous medium can include pH adjusting agent such as sodium hydroxide. The adhesive composition, upon curing, can have a porosity of 10-50%. The adhesive composition, upon curing, can include a plurality of pores. The adhesive composition can be porous, and upon curing can have a plurality of pores having a pore size of between 20 μm to 200 μm. Methods of producing the adhesive composition are disclosed. Methods of using the adhesive composition are further disclosed.

A system for preparation of an implant and ab interno insertion of the implant into an eye including a handle having one or more actuators and an elongated shaft having an outer sheath and an elongate member positioned within a lumen of the tubular outer sheath. The system includes a recess sized for holding a patch of material fixed relative to the handle and a cutting member movable relative to the handle and to the recess. The cutting member cuts the patch of material into an implant as the cutting member moves towards a cutting configuration. The implant, once cut, is axially aligned with the lumen of the tubular outer sheath. The inner elongate member is movable relative to the tubular outer sheath to advance the implant into a deployment position in the lumen of the tubular outer sheath for delivery into the eye. Related devices and methods are provided.

Embodiments of an implantable prosthetic valve are disclosed. The valve can have an annular frame having a plurality of frame members and a skirt assembly. The skirt assembly can include a laminate having a fabric layer sandwiched between a first covering member and a second covering member. At least one surface of the fabric layer can be exposed at one or more windows in the second covering member. The skirt assembly can be coupled to the annular frame by a suture extending through the fabric layer at the one or more windows and around at least one of the plurality of frame members. Methods for making such an implantable prosthetic valve are also disclosed.

The invention discloses uniform-particle-size silk fibroin microspheres and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution is taken and subjected to trypsin digestion to obtain modified silk fibroin sediment, and the modified silk fibroin sediment is dissolved in an aqueous solution of a beta-folding inhibitor to obtain a silk fibroin solution serving as a dispersed phase; emulsifying through an external pressure type membrane emulsifier to obtain an emulsion containing the silk fibroin emulsion droplets; adding a cross-linking agent into the emulsion to carry out cross-linking reaction, and then adding a crystallization inducer to induce crystallization to obtain primary silk fibroin microspheres; and performing vacuum freeze drying to obtain the silk fibroin microspheres. Silk fibroin is subjected to enzyme digestion modification, silk fibroin fragments with high beta-folding content are reserved, and high structural stability is achieved; the crystallinity of the microspheres is improved by adopting a membrane emulsification mode and combining a multiple crystallization induction method, and the silk fibroin microspheres with uniform particle size and smooth surfaces are obtained.

The invention relates to the technical field of biomedicine, in particular to stem cell exosome-loaded bi-crosslinked hydrogel as well as preparation and application thereof, and the bi-crosslinked hydrogel comprises the following components in parts by weight: 50-90 parts of a matrix polymer; 0.1 to 5 parts of an active load; 1 to 10 parts of cyclodextrin; 5 to 20 parts of modified chitosan; 0.01 to 1 part of a photoinitiator; and 0.01 to 0.5 part of growth factors. Through double-network design of physical / chemical primary cross-linking and photo-initiation secondary cross-linking, the bottleneck that the mechanical property and the machinability of the traditional single cross-linking hydrogel are difficult to consider at the same time is overcome; meanwhile, the human umbilical vein endothelial cell exosome is introduced, and efficient loading, uniform dispersion and degradation coupling slow release of the exosome are realized by virtue of beta-cyclodextrin inclusion and a double-crosslinking network. Therefore, the problems of mechanics, degradation, cross-linking speed or tissue adhesion of cross-linked hydrogel in the prior art are solved.

The invention relates to a regenerated decellularized adipose derivative as well as a preparation method and application thereof. The preparation raw material of the regenerated decellularized adipose derivative is prepared from a vascular matrix component (SVF) separated from human adipose tissue through decellularization treatment and freeze drying; the prepared regenerated decellularized adipose derivative is a porous material, the porosity is greater than 90%, and the aperture is 50-200 [mu] m; the composition comprises the following components in percentage by weight: 60-70% of collagen, 15-20% of elastin, 1 * 10 < 10 >-5 * 10 < 10 > particles / g of exosome, less than or equal to 5 ng / mg of DNA (deoxyribonucleic acid) residue, more than or equal to 30 ng / g of VEGF (vascular endothelial growth factor) and more than or equal to 15 ng / g of FGF-2 (fibroblast growth factor). The prepared regenerated decellularized adipose derivative is low in DNA residual quantity, rich in growth factors and capable of meeting the requirement for deep tissue filling due to the fact that the collagen content and the elastin content are high.

The invention discloses bioactive macroporous hydrogel as well as a preparation method and application thereof. The bioactive macroporous hydrogel comprises hydrogel particles and a bioactive load loaded on the hydrogel particles, the hydrogel particles are obtained by mechanically extruding a hydrogel matrix, and the hydrogel matrix is formed by compounding sodium alginate, agarose and methacrylated hyaluronic acid through hydrogen bond crosslinking and photo-crosslinking; the bioactive load comprises: a) small extracellular vesicles of which the surfaces are modified with macrophage targeted CRV peptides; and b) a PLGA (poly (lactic-co-glycolic acid)) microsphere loaded with a cartilage inducer Kartogenin. The bioactive macroporous hydrogel disclosed by the invention has an internally communicated macroporous network, is beneficial to cell infiltration, blood vessel ingrowth and nutrient substance diffusion, and overcomes the defects that the traditional hydrogel is small in pore size and limits cell behaviors. According to the bioactive macroporous hydrogel disclosed by the invention, dual bioactive loads are adopted to synergistically promote tendon-bone healing, so that the treatment effect is better.

The invention discloses preparation and application of dual-network hydrogel. The dual-network hydrogel comprises the following components: (Z1) a spinal cord acellular matrix network DSM formed by self-assembly of a spinal cord acellular matrix dSECM; and (Z2) a metal-polyphenol network MPN formed by coordination of metal ions and polyphenols; wherein the DSM and the MPN are cross-linked through hydrogen bonds to jointly form a double-network structure. DSM provides a microenvironment suitable for cell survival and growth for the dual-network hydrogel and induces stem cells to differentiate into neurons, and MPN provides the dual-network hydrogel with biological effects of inhibiting inflammation and accumulation of reactive oxygen species (ROS). The dual-network hydrogel has the advantages of the two components, the active component neurotrophic factor and the inhibitor loaded on the dual-network hydrogel can regulate and control immune response after SCI, improve neuroinflammation microenvironment and promote nerve repair, and a new strategy is provided for in-situ tissue repair of SCI.

Disclosed in the present invention are a gel having a regulatable free water content and morphology, a preparation method therefor, and the use thereof. The method comprises: S1, preparing a solution A from a natural polymer material; S2, mixing a small molecule material containing a positively charged polar group with the solution A to obtain a composite solution B; S3, mixing the composite solution B with a regulator to obtain a composite solution C, stirring same, then adding a condensing agent to the reaction system, and carrying out a curing crosslinking reaction; and S4, removing the regulator and the condensing agent to obtain the gel. By adjusting the concentrations of the natural polymer material and the regulator, gels having different morphologies can be prepared; the gel undergoes a physical process to remove hydrogen bond interaction between the gel and free water, so that the content of free water attached to the surface of the gel can be regulated, thereby regulating the physical and chemical properties of the gel. The gel of the present invention has regulatable morphology and controllable elastic modulus, and has many advantages such as good lubrication effect, good biocompatibility and biodegradability, capability of being biologically active, and capabilities of stimulating cell growth and accelerating wound repair.

The present invention relates to the technical field of protein engineering and provides a recombinant humanized type III collagen having a triple helix structure, a preparation method therefor, and a use thereof. The amino acid sequence of the recombinant humanized type III collagen comprises n core units, wherein n is 1 or an integer greater than 1, and the amino acid sequence of the core unit is as shown in SEQ ID NO: 1. The present invention further provides a method for constructing an expression system and a method for preparing a recombinant humanized type III collagen. The present invention further provides an actual industrial use on the basis of the characteristics of the protein. The recombinant humanized type III collagen provided by the present invention has high purity, good stability, and high biological activity. Experiments have shown that the recombinant humanized type III collagen has various biological activities such as promoting cell proliferation and migration.

The invention discloses composite hydrogel bio-ink with a function of space-time delivery of double growth factors as well as a preparation method and application of the composite hydrogel bio-ink, and belongs to the technical field of biomedical materials and tissue engineering. According to the preparation method, imidazolyl-modified methacrylated gelatin and double-bonded PF127 are compounded, and meanwhile, free vascular endothelial growth factors and connective tissue growth factor-loaded PLGA microspheres are entrapped in a system, so that the bio-ink with excellent printability, high adhesion, swelling resistance and space-time delivery function is formed; the bio-ink physically has the advantages of high printing fidelity, low swelling property, high mechanical strength and strong tissue adhesion of a synthetic material; in biology, the system can be used as an intelligent carrier to realize programmed space-time delivery of various growth factors, so that complex cell biological behaviors are accurately arranged, and real regeneration of functional tissues is guided.

The invention discloses a bionic heterogeneous composite structure bone implant and a preparation method thereof, and relates to the technical field of medical implants.The bionic heterogeneous composite structure bone implant comprises an inner-layer cross-shaped structure, a middle-layer negative Poisson's ratio structure and an outer-layer honeycomb structure, and the cross-shaped structure, the negative Poisson's ratio structure and the honeycomb structure jointly form a bionic composite structure; and immersing the bionic composite structure subjected to double-network crosslinking into a drug-containing solution for post-treatment and drug loading to obtain the bionic heterogeneous composite structure. According to the invention, bionic adaptation and intelligent optimization of mechanical properties are realized, and the stress shielding problem is solved; according to the invention, osseointegration and vascularization processes are obviously accelerated and enhanced, and leap-type improvement of biological activity is realized; a novel intelligent treatment mode based on in-vivo microenvironment response is developed, and on-demand and accurate drug delivery is achieved.

An acellular product may be derived from human placenta and may be used in various scenarios for wound healing. Because the product may be acellular, the product may be processed for storage and transportation with minimal degradation. The product may include various scaffolding such as biomaterials or human tissue, and the scaffolding may be infused with various plasmas and agents. The cell-free treatment may maintain the biological activity of many therapeutic agents found within cells and may possess multiple structural components to support cellular attachment. The structural components or scaffolds may function as a reservoir of highly diffusible chemotactic and cellular-programming factors that may be useful to treat injury and disease.

The invention belongs to the technical field related to tumors, and discloses a preparation method of self-developing embolism microspheres. Comprising the following steps: producing calcium alginate microspheres through cross-linking, obtaining polyelectrolyte hydrogel microspheres through electrostatic complexation, and obtaining the self-developing embolism microspheres through amino cross-linking. According to the invention, sodium alginate and polyamino acid are used as raw materials for preparation, so that the preservative is safe and non-toxic and has biodegradability, and the degradation product has no toxic or side effect on human body. The microspheres are prepared by adopting an electrostatic spraying method, organic solvents are not used, and the microspheres are green and environment-friendly and have no toxic residues.

The invention provides high-toughness chitosan hydrogel and a preparation method thereof, and belongs to the technical field of chitosan materials. Under a specific electric field driving condition, a chitosan solution realizes molecular chain oriented migration and in-situ deposition in an acid environment, and a three-dimensional network with dense arrangement and a gradient structure is constructed. The chitosan hydrogel disclosed by the invention has excellent performance in the aspects of tensile strength, elongation at break, toughness, shear resistance and the like which exceed those of chitosan hydrogel prepared by most methods, and has good biocompatibility and application potential. The programmable regulation and control of the structure and performance of the chitosan hydrogel can be realized by regulating and controlling the pH value, the electric field intensity, the current density, the deposition time and the like of the system. The high-toughness chitosan hydrogel prepared by the method is suitable for various biomedical scenes, such as bionic soft tissues, bone repair scaffolds, flexible electronic devices and other wet application scenes with high mechanical property requirements.

This invention discloses a composite coating for the tooth surface of an atlantoaxial PEEK interbody fusion cage, relating to the field of medical materials technology. The composite coating for the atlantoaxial PEEK interbody fusion cage comprises a transition layer covering the tooth surface of the atlantoaxial PEEK interbody fusion cage and a hydroxyapatite coating on the surface of the transition layer; the transition layer is obtained by mixing modified nano-hydroxyapatite and modified PEEK; the hydroxyapatite coating is obtained by plasma spraying of nano-hydroxyapatite. This application sets a transition layer between the PEEK and the hydroxyapatite coating, effectively addressing the problem of easy coating detachment due to the difference in physicochemical properties between the PEEK and hydroxyapatite coatings. The composite coating prepared in this application improves the bonding strength between the coating and the substrate, and the prepared material can be used in biological organisms for a long time.

The invention provides methods of treating an eye disorder and compositions for use therein, the methods comprising applying a crosslinkable liquid composition onto an anterior corneal surface of said eye using a mold; crosslinking the crosslinkable liquid composition on the anterior corneal surface of the eye, thereby obtaining a crosslinked composition on the anterior corneal surface of the eye; and correcting the curvature of the crosslinked composition.

The invention belongs to the field of biomedical materials, and discloses a hydrogel patch with piezoelectricity, conductivity and high adhesion and a preparation method and application of the hydrogel patch. Polyvinyl alcohol (PVA) and acrylic acid (AA) serve as polymer frameworks of the hydrogel patch; the invention relates to a nano-zinc oxide / poly (3, 4-ethylenedioxythiophene) / poly (styrenesulfonic acid) (PEDOT: PSS) composite conductive adhesive, which comprises nano-zinc oxide (ZnO) as a piezoelectric material, poly (3, 4-ethylenedioxythiophene)-poly (styrenesulfonic acid) (PEDOT: PSS) as a conductive material, and N-hydroxysuccinimide acrylate (NHS-AA) as an adhesive functional monomer. According to the invention, active ester groups in the N-hydroxysuccinimide acrylate are utilized, so that the patch can quickly form covalent amido bonds with amino groups on the surface of a tissue in a dry state, firm adhesion is realized, local nerve repair can be effectively promoted through a piezoelectric effect, a novel ideal material is provided for disease treatment, and the patch has wide application prospects. Wide clinical application prospects are realized in the field of biological medicines.

The invention provides a poly-L-lactic acid microsphere. The poly-L-lactic acid microsphere is obtained by performing membrane emulsification on a poly-L-lactic acid-polyethylene glycol copolymer synthesized by taking lactide and polyethylene glycol as raw materials. According to the L-polylactic acid microspheres disclosed by the invention, the uniformity, the stability and the biocompatibility of the microspheres are improved, and meanwhile, the synthesis of skin collagen is promoted.

The invention provides a method for preparing vaterite type nanoscale calcium carbonate with ardealite as a raw material and application of the method, and relates to the technical field of inorganic nano-material preparation.The method comprises the following steps that firstly, efficient dissociation of calcium ions in the ardealite is achieved through a phase transfer method, then digestion treatment is conducted on a leaching solution, and the leaching solution is obtained; a crystal morphology regulating agent is further introduced to construct a mineralized microenvironment, and then through a carbon dioxide carbonization reaction, vaterite type nano-scale calcium carbonate with a specific average particle size (200-400 nm) is prepared. According to the preparation method, natural high polymer materials such as collagen are introduced in the preparation process to serve as green crystal form guiding agents, so that the coordination effect of the natural high polymer materials and calcium ions can be utilized, the crystal morphology can be effectively regulated and controlled, and the biocompatibility of the nano material is improved; and the application possibility of calcium carbonate in the biomedical fields of drug carriers, bone repair materials and the like is expanded.

The invention discloses composite hydrogel as well as a preparation method and application thereof. The composite hydrogel comprises a hydrogel matrix and a liposome loaded on the hydrogel matrix, the hydrogel matrix comprises a methacrylated natural protein, and the liposome is loaded with a drug; the medicine comprises a medicine for promoting cartilage differentiation and an anti-inflammatory traditional Chinese medicine monomer. According to the invention, through a liposome entrapment technology, the stability and sustained release ability of the insoluble traditional Chinese medicine monomer and the cartilage differentiation induction drug are enhanced. The particle size of the liposome is controllable, the liposome is well combined with a methacrylated natural protein network, a drug sustained release bin can be formed in the hydrogel, long-time and local precise release is achieved, the drug utilization efficiency is improved, and systematic toxic and side effects are avoided.

The invention discloses a glucose responsive composite material as well as a preparation method and application thereof. The composite material sequentially comprises porous metal, a polydopamine layer and a mesoporous silicon dioxide coating from inside to outside, the porous metal is coated with the polydopamine layer, the polydopamine layer is coated with the mesoporous silicon dioxide coating, and preparation raw materials of the mesoporous silicon dioxide coating comprise aminated mesoporous silicon dioxide, aspirin, 4-carboxyphenylboronic acid and sodium alginate. The composite material disclosed by the invention has blood sugar concentration dual-mode response capability, aspirin is suddenly released in a hyperglycemia environment, up-regulation expression of anti-inflammatory factors is remarkably promoted, and an anti-inflammatory effect is dominated; in a normal blood glucose environment, aspirin is slowly released, and the expression of osteogenic factors is remarkably up-regulated, so that osteogenic differentiation is promoted as a leading effect; the dual-mode response realizes accurate regulation and control of ASP dosage, organically combines bone formation promotion and anti-inflammatory functions, and provides a brand new solution for patients with bone defects, especially patients with bone defects caused by diabetes mellitus.

Provided are a medical hydrogel containing ultrasonically-imageable bubble microspheres and a preparation method thereof. The medical hydrogel containing the ultrasonically-imageable bubble microspheres is formed by in-situ crosslinking of a polyethylene glycol precursor solution and a poly-amino crosslinker solution containing bubble microspheres, the polyethylene glycol precursor solution consisting of a multi-arm polyethylene glycol derivative and a buffer solution A.

The invention discloses a magnetoelectric stent for realizing bone repair under external field driving and a preparation method and application thereof.The stent comprises a stent body based on a triple-period minimal curved surface structure and a magnetoelectric nano-particle coating, and the stent body comprises a convoluted and / or through hole structure; and the magnetoelectric nanoparticle coating is loaded on the inner surface of at least part of the pore structure. The magnetoelectric stent is suitable for providing better mechanical support for the early stage of bone repair of large-area bone defects, meanwhile, under the synergistic effect of magnetoelectric stimulation, osteogenesis and angiogenesis coupling can be promoted, and bone defect repair under the complex conditions of oral clinical alveolar bone vertical bone increment and systemic diseases such as inflammation and the like is achieved.

A method of providing a channel in nervous tissue filled with an aqueous gel for implantation of a microelectrode or other medical device lacking sufficient physical stability for direct implantation by insertion, comprises providing an apparatus comprising an oblong rigid pin covered by a dry gel forming agent; locating a target in the tissue; defining a straight insertion path a desired tissue insertion point and the target; aligning the pin with its end foremost with the insertion path; inserting the pin into the tissue to a position near or at the target; allowing sufficient time to pass for a gel to be formed around the pin, withdrawing the pin. Also disclosed is a corresponding channel; a method of implantation of a microelectrode or microprobe into nervous tissue via the channel; a corresponding method of implantation of living cells; a corresponding apparatus for forming the channel.