56 results about "Amnion" patented technology

The invention provides an application of a cross-linked decellularized bovine amniotic membrane (CAM) and manganese carbon dots (Mn CDs) combined in preparation of an ophthalmic medical material for alkali burn cornea repair. The invention further provides a compound for repairing the alkali burn cornea. Cell and animal experiments show that the compound CDs (at) CAM has good biocompatibility and strong antioxidant and anti-inflammatory effects, can significantly promote corneal epithelium regeneration and inhibit corneal neovascularization and scar generation, and provides a new strategy for treatment of alkali burn cornea and various corneal epithelium injuries and diseases.

The invention discloses a composite amnion for improving contracture after tissue repair and a preparation method of the composite amnion. The composite membrane comprises a recombinant humanized collagen nanofiber membrane located in the middle layer and human decellularized amniotic membranes attached to the two faces of the nanofiber membrane. The composite membrane is prepared by preparing a recombinant humanized collagen nanofiber membrane through an electrostatic spinning technology and pressing the recombinant humanized collagen nanofiber membrane and a human decellularized amniotic membrane. The composite membrane has excellent mechanical properties and biological activity. Animal experiment results show that the composite film can effectively inhibit capsule contracture induced by radiotherapy, and the comprehensive effect of the composite film is obviously superior to that of a single-component material in the aspects of inflammation resistance, fibrosis resistance, degradation speed delaying, capsule compliance improving and the like. The composition can be used for improving the capsule contracture of the breast prosthesis, especially the capsule contracture induced by radiotherapy.

The invention aims to provide a preparation method of amnion-derived mesenchymal stem cells with high collagen expression. The preparation method comprises the following steps: step 1, reagents and materials: amnion tissues: obtaining a fresh amnion from a healthy puerpera after delivery; the PBS is used for cleaning amniotic tissues; collagenase: type I collagenase, which is used for digesting amniotic tissues to separate mesenchymal stem cells; a cell culture medium is a DMEM / F12 culture medium, and 10% of fetal calf serum and 1% of penicillin-streptomycin double antibodies are added; the transforming growth factor TGF-beta is used for promoting the expression of mesenchymal stem cell collagen; and the fibroblast growth factor FGF can assist in promoting cell proliferation and collagen expression. The invention aims to provide the preparation method of the amniotic membrane-derived mesenchymal stem cells for highly expressing collagen, and the amniotic membrane-derived mesenchymal stem cells can be widely applied to treatment of immune system diseases, reproductive system diseases, skin system diseases, skeletal system diseases, nervous system diseases, digestive system diseases, respiratory system diseases, circulatory system diseases and urinary system diseases. Particularly, the effects of immunosuppression and tissue repair are achieved.

This disclosure generally relates to methods of preparing a graft for wound healing. In some embodiments, the methods comprise obtaining a donor placenta and isolating tissue comprising amnion and / or chorion from the donor placenta. In other embodiments, the methods comprise washing the isolated tissue with a cleansing solution. Other embodiments, still, are directed toward applying a current to the isolated tissue and / or cleansing solution to produce the graft for wound healing.

The utility model relates to the technical field of cervical cerclage, in particular to a cervical cerclage support for cervical insufficiency, which comprises a protective ring, a push rod, first connecting blocks, a fixing rod, a silica gel pad and a second connecting block, the push rod is slidably connected in the protective ring, the first connecting blocks are connected at the upper end of the push rod at equal intervals, and the fixing rod is connected with the silica gel pad. Fixing rods are rotationally connected to the first connecting blocks, silica gel pads are jointly connected between the fixing rods, and second connecting blocks are rotationally connected to the fixing rods. The fixing rod and the silica gel pad are installed, due to the fact that the amniotic sac is protected by the silica gel pad, the silica gel pad can effectively isolate direct contact between a suturing instrument and the amniotic sac, the softness and the elasticity of the silica gel pad can adapt to the shape of the amniotic sac, comprehensive protection is provided, and through protection of the silica gel pad, the suturing instrument can be prevented from being damaged. A doctor can perform suture operation more trustingly, the safety and success rate of an operation are effectively improved, and the operation risk is reduced.

The invention discloses a ritodrine-loaded decellularized amnion matrix temperature-sensitive hydrogel and pharmaceutical application thereof, i.e., an amnion is subjected to decellularization treatment and then is digested by pepsin to obtain blank gel, and then drug loading is performed to obtain drug-loaded gel for treating premature delivery. The ritodrine-loaded vagina temperature-sensitive gel with high biocompatibility is prepared by taking a premature delivery treatment drug ritodrine commonly used in clinic as an active compound and taking an acellular amniotic membrane as a carrier, so that the enrichment of the drug in the uterus is improved, and the systematic exposure of the drug and the side effects caused by the systematic exposure are reduced. The invention provides a medicine delivery and treatment strategy based on the uterus first-pass effect for premature delivery treatment, and provides a simple and effective technical scheme for developing clinically effective premature delivery treatment medicines and reducing side effects of clinical medicines.

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located on top of and within the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high levels of laminin-5, a cell adhesion bio-active factor shown to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.

The invention discloses a gynaecology and obstetrics intelligent visual midwifery system based on an ultrasonic technology, and relates to the technical field of ultrasonic midwifery, the system constructs a two-dimensional coordinate system and extracts ultrasonic echo total time and respiratory phase data, and fetal membrane structure data and fetal physiological data are generated through dimensionless processing and data analysis. And constructing an amniotic sac thickness gradient index dyn according to the thickness gradient change to evaluate the structural stability of the amniotic sac. And if the fetal respiratory phase drift index psh is evaluated to be unstable, calculating the fetal respiratory phase drift index psh, and jointly constructing a comprehensive parturition risk index Rco with the amniotic sac thickness gradient index dyn to reflect the potential parturition risk of coupling of the fetal stress level and the membrane structure variation. And the system generates different types of intervention instructions according to the evaluation result of the comprehensive parturition risk index Rco. Multi-dimensional data mapping and layer display are achieved, linkage display of a risk thermodynamic diagram and a breathing phase curve is supported, and a visual and traceable dynamic midwifery decision basis is provided for medical staff.

The invention discloses an amniotic epithelial cell-derived exosome for treating gestational hypertension and application thereof, and belongs to the technical field of biological medicines. The exosome (hAECs-exos) is obtained by secreting and extracting human amniotic epithelial cells (hAECs), the particle size distribution of the exosome (hAECs-exos) is 30-150 nm, and the exosome (hAECs-exos) expresses exosome specific markers CD9, CD63 and CD81. The invention also provides a preparation method of the exosome. The preparation method comprises the following steps: isolated culture of hAECs, and ultracentrifugal extraction and identification of the exosome. Animal experiments prove that when the exosome is applied to a gestational hypertension disease model animal in an intravenous injection mode, the blood pressure and urine protein level of the gestational hypertension disease model animal can be remarkably reduced, and the fetal rat dysplasia condition is improved. The invention provides a brand-new and effective biological preparation candidate scheme for the treatment of the gestational hypertension disease.

A cell population including macrophages that are CD11b positive, F4 / 80 positive, CD180 positive, and CD9 positive and that are derived from a tissue selected from the group consisting of placenta, umbilical cord, and amnion with a percentage of 75% or more, the cell population having cell viability of 80% or more.

The invention relates generally to the fields of biology and health sciences. More particularly, the invention relates to compositions and methods for modulating cellular physiology and pathological processing using a combination of compounds that can be found in amniotic membrane tissue and umbilical cord tissue preparations.

The invention relates to the technical field of medical teaching devices, and discloses a cervical cerclage teaching practice device for simulating cervical insufficiency, which comprises a pig tripe water bag model and a base, and is characterized in that the pig tripe water bag model comprises an external wrapper for simulating cervical insufficiency and an internal filler for simulating an amniotic sac; the pylorus of the pig tripe in the model well simulates the special anatomical structure of the inner opening of the human cervix uteri and the texture of the human cervix uteri, meanwhile, the rubber sleeve simulates the state of the amniotic sac vividly, and the model vividly presents the state of incomplete cervix uteri functions. Meanwhile, the height of the base can be adjusted according to needs to adapt to trainees with different heights or simulate different cervix positions.

The invention relates generally to the fields of biology and health sciences. More particularly, the invention relates to compositions and methods for modulating cellular physiology and pathological processing using a combination of compounds that can be found in amniotic membrane tissue and umbilical cord tissue preparations.

This invention discloses an amniotic membrane mechanical tensile loading measurement device, relating to the field of membrane biomaterial research technology. It includes a fixed frame, a first clamping block assembly, a second clamping block assembly, a driving mechanism, and a monitoring device. The first and / or second clamping block assemblies are slidably disposed within the fixed frame, and the driving mechanism is fixedly disposed on the fixed frame. The driving mechanism can drive the first and second clamping block assemblies to move closer or further apart. Each clamping block assembly includes an upper clamping block, a lower clamping block, and a clamping locking mechanism. One of the upper and lower clamping blocks has a meshing groove, and the other has a meshing tooth. The two ends of the amniotic membrane are respectively fixed to the two clamping block assemblies. The clamping locking mechanism is a magnetic suction element. A microscope can be aligned with the amniotic membrane between the first and second clamping block assemblies. The monitoring device is used to monitor the operating parameters of the driving mechanism. This invention enables real-time in-situ observation in conjunction with a microscope and provides a stable clamping effect.

This invention relates to the field of obstetric nursing technology, and in particular to an obstetric nursing device for ruptured membranes with a negative pressure balancing function. The device includes an insertion tube, an endoscope fixedly mounted on the upper rear end of the insertion tube, and a membrane rupture assembly located at the rear end of the insertion tube. The membrane rupture assembly includes a baffle plate fixed to the rear end of the insertion tube, with multiple guide membrane holes inside the baffle plate. A blade groove is formed on the rear end face of the baffle plate, and a membrane rupture blade is movably mounted inside the blade groove. The baffle plate of this invention effectively blocks the fetus inside the amniotic sac, allowing the soft outer wall of the amniotic sac to enter the guide membrane holes, thus preventing damage to the fetus during membrane rupture. Simultaneously, the membrane rupture blade is located inside the insertion tube, ensuring that neither the fetus nor the mother is harmed during membrane rupture.

The invention relates generally to the fields of biology and life sciences. More particularly, the invention relates to compositions and methods for modulating cellular physiology and pathological processing using a combination of compounds that can be found in morselized amniotic membrane tissue and morselized umbilical cord tissue preparations.

The main purpose of this technology is developing a collagen matrix or membrane with antimicrobial properties, comprising collagen from fetal amniotic membranes and metallic nanoparticles. Additionally, its manufacturing procedure is described.

The present invention provides an amniotic sac protection device based on 3D printing and a preparation method thereof. Among them, the amniotic sac protection device includes a sheath tube and a support sac formed by 3D printing; the support sac is detachably installed at the upper end of the sheath tube, and the inside of the support sac is a cavity; a liquid injection channel communicating with the internal cavity of the support sac is arranged in the sheath tube, and a liquid injection joint communicating with the liquid injection channel is further arranged at the liquid injection port of the liquid injection channel. The amniotic sac protection device has a simple structure, which is convenient for the disassembly and installation of the support sac and the sheath tube, has high surgical hygiene and safety, and the support sac can be personalized designed based on 3D printing of the support sac, can better meet the needs of cervical cerclage surgery for different patients, makes the surgical operation more convenient and efficient, and reduces the surgical risk.

A sterile micronized composition prepared from intact human placental tissue that is configured for wound healing. The sterile micronized composition includes micronized human amnion; micronized human chorion; and micronized human intermediate spongy layer. In certain aspects, the composition is prepared from intact placental tissue comprising a human amnion layer, human chorion layer, and an intact human intermediate spongy layer positioned between the human amnion layer and human chorion layer such that immediately before micronization the human amnion layer and the human chorion layer are not separated from one another.

The present invention relates to functionally improved epithelial stem cells. The epithelial stem cells with improved functionality by a method of the present invention are prepared without genetic modification, thus being less likely to cause side effects due to DNA delivery / modification, are derived from the amniotic membrane in which no tissue-compatible antigen (factor) is expressed, thus having no rejection, and exhibit improved cell migration and would healing effects due to the improved functionality. Therefore, the epithelial stem cells can be used for the regeneration of various tissues, including the cornea, amniotic membrane, skin, and the like, and particularly can be used for the prevention or treatment of premature rupture of the amniotic membrane.

Compositions comprising perforated, unseparated amnion / chorion; perforated, separated amnion; and perforated, separated chorion derived from the placenta and methods of preparing and using those compositions are provided. Perforation of placental tissue before or during processing may allow for one or more benefits such as more efficient removal of blood remnants, retention of wound healing and tissue regeneration components, better handling characteristics, or improved healing capacity. The present invention also includes methods of healing a wound of the skin, eye, nerve, tendon, or dura comprising applying the perforated compositions of the invention to the wound.

The invention provides an activity-enhanced amniotic membrane matrix as well as a preparation method and application thereof. According to the invention, on the basis of amniotic cells, genes for coding IL-4 and IL-22 growth factors are introduced into the amniotic cells by using a gene editing technology, and the genes are secreted outside cell membranes by using extracellular signal peptides, so that the filling material containing high-activity biological factors is prepared, the cell proliferation, migration and tissue regeneration are remarkably promoted, and the biological factors have good biocompatibility. The wound healing and tissue repairing processes are accelerated.

This invention provides a fluid therapeutic placental product comprising placental cells and a placental dispersion comprising placental factors. The placental cells and the placental dispersion are derived from placental tissue. A placental tissue can optionally be an amnion, chorion, or a trophoblast-depleted chorion. The placental product of the present invention is useful in treating a patient with a tissue injury (e.g. wound or burn) by applying the placental product to the injury. Similar application is useful with ligament and tendon repair and for engraftment procedures such as bone engraftment.

The invention relates to the field of biological materials, and discloses a bio-based composite material composed of genetically modified mesenchymal stem cells and a mammalian extracellular matrix, and the bio-based composite material is prepared from mesenchymal stem cells obtained by modifying specific targets such as mTOR, FAP-alpha, COL3A1 and DPP4 genes by using a gene silencing technology. The mammals comprise pigs, sheep, cows and humans, the gene silencing technology is an s i RNA technology or shRNA technology, the mTOR and FAP-alpha gene targets are subjected to low expression, the COL3A1 gene targets are subjected to high expression, the DPP4 gene targets are subjected to low expression, the expression of the DPP4 gene targets is reduced, the expression of the DPP4 gene targets is reduced, and the expression of the DPP4 gene targets is reduced. A stabilizer is added in the extracellular matrix extraction process. The performance of the composite material is improved by precisely regulating and controlling genes, and tissue regeneration and repair are promoted; the extracellular matrix extraction method has multiple advantages and good biological activity; the biocompatibility and the mechanical property of the material are improved by adopting various innovative means, and the application prospect in the fields of orthopedics and plastic surgery is wide.

Methods for producing extra-embryonic structures Described herein is a novel cell culture model of the post-gastrulation stage human amnion. Also described herein are methods for producing extra-embryonic structures in culture, in particular for producing an in vitro three-dimensional amnion model. The amnion models described herein, and amniotic membranes isolated from such amnion models, can be used in a variety of applications, such as in methods of drug screening, tissue engineering, regenerative medicine, wound healing and treating burns.

Cervical ripening catheters are disclosed, and methods for monitoring and measuring a change in intrauterine parameters without rupturing the amniotic sac. The catheter is provided with a uterine balloon, and a pressure sensor in pressure sensing communication with the uterine balloon. Sensing circuitry is configured to detect pressure applied to the pressure-sensor and communicate information relating to uterine contractions. The catheter may also be provided with sensors for determining fetal heart rate, oxygen saturation, respiratory rate, fetal blood pH or pelvimetry measurement. A working channel extends throughout the catheter to permit direct access to the amniotic sac by additional diagnostic or therapeutic tools.

Methods of treating conditions (e.g., cancer) in a subject using a canine-specific therapeutic composition are described herein. The canine-specific therapeutic composition is clear, safe, and physiologically and biologically active. Methods described herein may comprise administering a therapeutic amount of the canine-specific therapeutic composition which may comprise a micronized canine-specific allantoamnion membrane, in a solution. Thus, the present invention features canine-specific therapeutic compositions comprising placental-derived materials and methods of use.

The present invention provides a birth tissue derived implant for treating a subject. The birth tissue derived implant comprises a birth tissue and an agent exogenous to the birth tissue. The birth tissue is isolated from an amniotic sac, an umbilical cord or a placental plate. A method is also provided for repairing a defective tissue in a subject. The repair method comprises delivering the birth tissue derived implant to a surface of the defective tissue in the subject. The defective tissue may be an ocular membrane, a synovium, tendon, ligament, nerve, a cartilage or a bone. A method is further provided for preparing the birth tissue derived implant. The preparation method may comprise devitalizing or decellularizing a birth tissue from an amniotic sac, an umbilical cord or a placental plate, and adding an agent to the devitalized or decellularized birth tissue.

The invention relates to the technical field of stem cells, in particular to a preparation method of human placenta amnion pluripotent stem cells. The method specifically comprises the following steps: S1, tissue pretreatment and physical pre-layering; s2, carrying out mechanical-enzymatic synergistic dynamic dissociation; s3, gradient purification and washing; s4, performing soft matrix phenotype maintenance culture; according to the method disclosed by the invention, the separation efficiency of amniotic epithelial cells is improved and the enzyme exposure load is reduced through synergistic enzymolysis of Dispase II and Trypsin in combination with weak shearing force generated by the magnetic beads. Meanwhile, SB431542 blocks a signal channel to prevent EMT, a GelMA soft matrix maintains cell phenotypes, and Percoll density gradient centrifugation is matched to improve cell purity.

A sterile micronized composition prepared from intact human placental tissue that is configured for wound healing. The sterile micronized composition includes micronized human amnion; micronized human chorion; and micronized human intermediate spongy layer. In certain aspects, the composition is prepared from intact placental tissue comprising a human amnion layer, human chorion layer, and an intact human intermediate spongy layer positioned between the human amnion layer and human chorion layer such that immediately before micronization the human amnion layer and the human chorion layer are not separated from one another.