The invention discloses a culture method of in-vitro uniform
monolayer cells of prawns, and belongs to the technical field of
biology. The method comprises the following steps: (1) obtaining
lymphatic tissues of a last-stage
prawn at the early stage of shelling, completely stripping an outer membrane to obtain internal tissues, and
cutting the internal tissues into small tissue blocks; (2) cleaning the small tissue blocks, inoculating the small tissue blocks to a culture plate coated with a
gelatin solution, adding a complete culture solution into an
inoculation system at intervals within 12 hours after
inoculation for culture, replacing the complete culture solution 24-48 hours after
inoculation, and inducing tissue cells to continuously migrate to form an in-vitro
cell layer; (3) blowing and beating the
cell culture to obtain a
cell suspension, and centrifuging to obtain a cell
monolayer; and (4) suspending the cell
monolayer by adopting a complete culture solution, then inoculating the cell monolayer into a culture plate coated with a
gelatin solution, and culturing to obtain the in-vitro uniform monolayer cells of the prawns. According to the method, the
prawn monolayer cells which are long in in-vitro survival time, uniform and stable and are cultured
in vitro can be obtained, and an important tool is provided for related
virology research.