213 results about "Epithelium" patented technology

The invention relates to the technical field of image classification, in particular to a training method and system of a cervical pathological image classification model based on multiple modes. The method comprises the following steps: acquiring a cervical tissue image and carrying out tissue structure segmentation, forming a nucleus-interstitial-epithelium three-distribution framework, collecting development historical data, confirming a prediction trend of each layer, carrying out environment field simulation through the image, generating a simulated cervical environment field, and carrying out hierarchical evolution prediction on the framework. Evolution mapping images are generated according to the evolution data and classified, finally, a visual basic model is obtained through combined modeling training, image-text fusion is achieved, and a cross-center deployment model system is generated. According to the method, vision-language combined modeling is realized, and the stability and controllability of the whole model structure in image space deformation modeling, semantic cross-modal alignment construction and task-level response flow scheduling are improved.

The invention discloses bifidobacterium animalis and application thereof in allergic rhinitis products, and belongs to the technical field of microorganisms. The technical problem to be solved is that the existing common allergic rhinitis treatment means cannot meet the requirements of patients on safety, long-acting effect and treatment cycle. The invention provides an animal bifidobacterium subsp. Lactis Def +, and after an effective dose of animal bifidobacterium subsp. Lactis Def + is applied to a subject, the animal bifidobacterium subsp. Lactis Def + is found to be capable of remarkably relieving the allergic rhinitis symptom. Specifically, the symptoms of sneezing, dry nose and rhinocnesmus are reduced; the number of nasal septum mucosa intrinsic layer inflammation infiltration cells and nasal septum mucosa epithelium pathological changes are reduced; the IgA and IgE levels of serum are reduced; the levels of proinflammatory factors IL-4, IL-5 and IL-13 are reduced; the normal spleen index is maintained; the imbalance state of Th1 / Th2 and Th17 / Treg is effectively improved, and the body immunity is improved; a safe and long-acting scheme is provided for allergic rhinitis diseases.

The present invention relates to a part (2) intended to be used in association with an implant (4) which is itself intended to be secured in a jaw, this part comprising, in series from its distal end (20) towards its proximal end (21), a distal portion (22) for securing in the implant, an intermediate portion (23) for attachment to the epithelial and / or connective tissue, and a proximal portion (24), this part also comprising at least two zones whose surfaces have a different structure, the structure of the surface of at least one of these two zones, located in said intermediate portion (23), said first zone being ordered and repetitive, adapted to the growth of the epithelial tissue and / or the connective tissue and having engravings forming corrugations with a depth of less than 0.5 microns.

The invention relates to the technical field of pharmaceutical analysis, and particularly provides a characteristic chromatogram of epimedium villosum, a characteristic chromatogram construction method and an identification method. According to the method, an epimedium villosum medicinal material is pretreated, and the characteristic chromatogram of the epimedium villosum is identified by means of a chromatographic analysis technology; the epimedium villosum and the planted epimedium villosum are distinguished through the relative peak area and the characteristic peak of the chromatographic peak, so that the technical problem that the wild epimedium villosum and the planted epimedium villosum cannot be distinguished by the existing methods such as appearance identification, thin-layer identification and DNA identification is solved, and the correct source of the wild epimedium villosum is effectively ensured; the method has the advantages of simple identification method and accurate result.

Disclosed herein are methods and compositions for quantifying distinct prognostic and predictive contributions of tumor epithelium vs. tumor microenvironment in colorectal cancer to optimize therapy.

The invention relates to a traditional Chinese medicine composition for treating psoriasis as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials: herba portulacae, radix sophorae flavescentis, cortex dictamni, honeysuckle flower, fructus kochiae, cortex phellodendri, cacumen biotae, radix paeoniae rubra and radix arnebiae seu lithospermi. The traditional Chinese medicine composition has the advantages of scientific and reasonable formula, synergistic interaction, few components and controllable quality. In the formula, the compatibility of monarch, minister, assistant and guide is scientific and reasonable, anti-inflammatory and immunoregulation compatibility of heat-clearing, blood-cooling and itching-relieving medicines can effectively remove pathogenesis and enhance the itching-relieving effect, the effects of anti-inflammatory, anti-proliferation and local vasoconstriction are achieved, the advantages of effect enhancement and toxicity reduction are achieved, and the risk of relapse can be reduced. Therefore, epithelial tissue repair is promoted, skin keratinization dynamic balance is maintained, relapse of psoriasis is effectively prevented, and pain of patients is reduced.

Systems and methods for improving the image quality of images of epithelial tissue structures are disclosed. The systems include training a first cycle-GAN model and a second cycle-GAN model simultaneously, where the first cycle-GAN model is trained to remove noise from an image and the second cycle-GAN model is trained to learn the structure of the image. Additional systems and methods include deploying the trained cycle-GAN model to identify an unknown image segment and / or generate a protocol for following the identified skin care treatment recommendation for an identified image segment.

Methods for non-invasive or minimally invasive assessment of epithelial tissue structure are disclosed. Digital imaging and processing are used to identify cell locations. More specifically, an automated algorithm that may be used to identify epithelial tissue structure, and / or to specify the coordinates / locations of cells in the epithelial tissue structure, through non-invasive or minimally invasive imaging, and use of this information to extract values of epithelial structure related parameters are disclosed.

The invention discloses a symmetric nucleoside microneedle patch loaded with polymer vesicles as well as a preparation method and application of the symmetric nucleoside microneedle patch. The symmetric nucleoside microneedle patch loaded with the polymer vesicles comprises a guanosine-quadruplex hydrogel microneedle tip doped with drug-loading polymer vesicles, the raw material of the drug-loaded polymer vesicle comprises a polycaprolactone-polyglutamic acid copolymer; the guanosine-quadruplex hydrogel is prepared from the following raw materials: guanosine and KB (OH) 4. The microneedle patch is constructed on the basis of dynamic covalent bond and non-covalent interaction, has a sharp tip array capable of penetrating the epithelium and the lower layer of a mucous membrane, can efficiently deliver a drug to a canker lesion part by bypassing a mucous membrane barrier, can be controllably broken under a high ROS condition of an inflammation microenvironment, has the characteristics of high drug delivery efficiency and high accuracy, and can be used for preparing a medicine for treating the canker lesion part. The effect of effectively improving chemotherapy-induced oral ulceration can be achieved by reducing inflammatory response, relieving pain feeling and inhibiting pain sensitization.

According to the pluripotent stem cell-derived human mammary gland organoid and the construction method thereof, the human mammary gland organoid is obtained through the following multi-stage induction culture: first-stage culture: culturing for 1-3 days by using a first culture medium to obtain a surface ectoderm stage organoid expressing a surface ectoderm marker K8 / K18; second-stage culture: culturing for 1-3 days by using a second culture medium to obtain a mammary gland substrate-like organ which has bright epithelium and expresses mammary gland substrate markers EDAR and LEF1; third-stage culture: culturing for 3-5 days by using a third culture medium; fourth-stage culture: culturing for 3-5 days by using a fourth culture medium; a fifth culture medium is used for culture for 8-12 days to form an epithelial structure with basal cells and lumen cells, a mammary gland physiological microenvironment simulated by mesenchymal cells and vascular endothelial cells and a mature mammary gland organ with a lactation function. According to the invention, the mature mammary gland organ with a lactation function is successfully constructed.

The invention discloses a tissue engineering integrated trachea regeneration construction method based on a frame structure scaffold and application. A multi-tissue-structure integrated gas pipe is constructed based on a 3D printing bioelastomer frame structure support, and the complex structure and physiological functions of a natural gas pipe are highly simulated. The preparation method comprises the following steps: firstly, by utilizing the characteristic that temperature-sensitive hydrogel Pluronic F-127 becomes semi-solid gel at 37 DEG C and is liquefied at a low temperature of 4 DEG C, and by adopting a strategy that the temperature-sensitive hydrogel is sacrificed after occupying, the patterned distribution of chondrocytes and fibroblasts on a two-dimensional plane is realized; secondly, simulating a three-dimensional tubular trachea structure through a 2D-3D (two-dimensional to three-dimensional) rolling assembly strategy, and realizing the ordered regeneration of C-shaped cartilage ring / O-shaped fiber ring alternate distribution and axial strip-shaped fiber bundle triple structures under the skin of the nude mouse. And finally, successfully extracting the airway mucosa epithelium, separating and amplifying enough airway basal cells in vitro, and loading the airway basal cells to the inner wall of the regenerated trachea by utilizing the photo-crosslinking hydrogel, thereby realizing the function simulation of the airway epithelium and enhancing the physiological functionality.

Described herein are methods, systems, and programming for determining a tumor immunophenotype of an image of a tumor. Some embodiments include dividing an image into tiles depicting tumor epithelium and / or tumor stroma. For each tile, an epithelium-immune cell density and a stroma-immune cell density may be calculated based on a number of immune cells identified in the tumor epithelium and the tumor stroma, respectively. Based on the epithelium-immune cell density and the stroma-immune cell density, an inflammation type of the type may be determined, and a tumor immunophenotype may be determined based on each tile's inflammation type.

The invention provides a mouse middle ear epithelium 3D organoid and a construction method thereof, and is characterized in that the method comprises the following steps: a, taking a bubble structure of a mouse middle ear tissue, cleaning, digesting, filtering, centrifuging filtrate, collecting precipitate, and separating primary cells of the mouse middle ear; and b, inoculating the digested single cells and cell agglomerates into a 24-pore plate, wrapping with matrigel, and carrying out 3D culture, wherein a medium is a middle ear epithelium amplification medium. And culturing for about 3-5 days to obtain the mouse 3D middle ear proliferation type organoid. And performing differentiation culture by using a middle ear epithelium differentiation medium to obtain the mouse middle ear epithelium organ. According to the method, the 3D mouse middle ear epithelium organ is successfully constructed, the physiological and pathological processes of the middle ear are conveniently analyzed and researched, the interaction between the middle ear microenvironment and cells can be more accurately simulated, and the application range is wide as a model.

The invention relates to the field of bioengineering, in particular to a bilateral cleft lip mouse model based on Pitx2-Cre conditional knockout of Bmp4 as well as a construction method and application of the bilateral cleft lip mouse model. The invention aims to construct a stable bilateral cleft lip mouse model. Experiments find that the Bmp4 gene is expressed in the epithelium of the frontal nose process and the maxillary process at the early lip stage in E9.25. The spatio-temporal expression mode of the Pitx2 gene in the early primordium epithelium is highly consistent with the spatio-temporal expression mode of the Bmp4 gene. Based on this, the inventor constructs a Bmp4 gene knockout model driven by an endogenous Pitx2 gene promoter, and finds that the mouse model shows a 100% bilateral cleft lip phenotype. Compared with the reported Netin-Cre, the Nestin-Cre has the advantages that the Nestin The bilateral cleft lip model constructed by the method is more stable, can better represent genetic diseases of human cleft lip, and has important research significance.

The invention relates to the technical field of biology, in particular to a matrix-enabled mesenchymal stem cell aggregate and application, and the matrix-enabled mesenchymal stem cell aggregate is obtained by subculturing matrix-enabled mesenchymal stem cells in a 3D cell culture system. The matrix-enabled mesenchymal stem cells are obtained by subculture of mesenchymal stem cells in a cell culture matrix, and the cell culture matrix is prepared from 1-3 parts of hE-cad-Fc and 1-3 parts of hN-cad-Fc. Endogenous epithelium cadherin and nerve cadherin are highly expressed in cell aggregation cells, so that transcription factors are promoted to enter nuclei, the expression levels of anti-inflammatory immune regulation related factors and matrix degradation and cell migration related factors are up-regulated, and activated macrophages are promoted to be polarized to M2 type macrophages; the effects of anti-inflammation, immune regulation, tissue damage repair and the like of intravenous implantation of the matrix-enabled mesenchymal stem cell aggregate for treating diseases such as hepatic fibrosis, diabetes mellitus, nerve injury and the like are remarkably improved.

A reagent kit, staining method, and application for combining dual immunohistochemistry with elastin fiber staining. The reagent kit includes a combination of primary antibodies, consisting of anti-CK7 and anti-CD34 monoclonal antibodies from different species, such as rabbit anti-human CK7 monoclonal antibody and mouse anti-human CD34 monoclonal antibody. By selecting CK7 and CD34 as the primary antibody combination, the growth pattern of tumor epithelium can be accurately indicated, and the proliferation and thickening of alveolar septal fibrous tissue caused by tumors can be clearly revealed, as opposed to structural changes of alveoli induced by other factors. Combined with independent Victoria blue staining, the elastin fibers of the walls of arteries and veins, as well as the pleura of the lungs, can be clearly visualized, aiding in the assessment of the integrity of elastin fibers on the lung surface and in determining the extent of tumor infiltration.

The present invention relates to a traditional Chinese medicine composition for treating psoriasis, and its preparation method and use. The traditional Chinese medicine composition is prepared from the following raw materials: Portulaca oleracea, Sophora flavescens, Dictamni bark, Honeysuckle, Kochia scoparia, Phellodendron amurense, Platycladus orientalis leaves, Paeonia lactiflora, and Lithospermum officinale. The traditional Chinese medicine composition of the present invention has a scientific and reasonable formulation, synergistic efficacy, a small number of ingredients, and controlled quality. The combination of monarch, minister, adjuvant, and envoy in the present invention's formulation is scientific and reasonable. The combination of anti-inflammatory and immunomodulatory heat-clearing, blood-cooling, and antipruritic drugs can effectively eliminate the cause of the disease and enhance the antipruritic effect, exerting anti-inflammatory, antiproliferative, and local vasoconstrictive effects, with the advantages of increased efficacy and reduced toxicity, and can reduce the risk of recurrence. This promotes epithelial tissue repair, maintains the dynamic balance of skin keratinization, effectively prevents the recurrence of psoriasis, and reduces the suffering of patients.

A method for rapidly and efficiently inducing multiple human airway epithelium in vitro models for a long time relates to the technical field of organoid culture.The method comprises the steps that firstly, a clinical lung tissue sample is induced and differentiated to form a cilia inward lung airway organoid, the cilia inward lung airway organoid is digested into single cells, and multiplication culture is conducted through a basal cell culture medium; the obtained basal cells are subjected to induced differentiation to form cilia outward lung airway organs and a gas-liquid interface culture model. The two types of models can successfully simulate the functional cellular morphology of the human airway epithelium in vitro, can be used as experimental models for disease modeling, drug screening, toxicity testing and the like, and can simulate biological models of the human airway structure and function. The method can solve the problems that clinical samples are difficult to obtain and primary lung epithelial cells have limited passage times in the construction process of lung airway organs, and realizes long-term rapid and efficient induction of various human airway epithelium in-vitro models.

The invention discloses periodontitis targeted sustained-release gel based on epithelial training immunization and a preparation method thereof, and relates to the technical field of biological medicine, and the preparation method comprises the following steps: modifying poloxamer, and dissolving the modified poloxamer in anhydrous acetonitrile; the preparation method comprises the following steps: dissolving RGD peptide in a PBS (Phosphate Buffer Solution), adding N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, and activating to obtain an activated RGD peptide solution; adding the activated RGD peptide solution into the modified poloxamer solution, and reacting at room temperature to obtain RGD peptide modified poloxamer; the RGD peptide modified poloxamer solution is mixed with a beta-glucan solution and a TLR2 agonist solution, and the periodontitis targeted slow-release gel is obtained. The gel provided by the invention specifically targets gingival epithelial cells, reduces inflammatory response and promotes periodontal tissue regeneration, has good biocompatibility and slow release performance, and can effectively control periodontitis and prevent relapse.

The invention relates to the technical field of retina analysis, and discloses a fundus optical coherence tomography image feature point automatic positioning method, which comprises the following steps: acquiring optical coherence tomography images of a fundus disease patient at different disease course stages, constructing an image database containing a training set and a verification set, and determining the feature points of the fundus optical coherence tomography image on the basis of the image database. And carrying out feature layer segmentation on the image by adopting a U-Net convolutional neural network. And determining a blood vessel shadow position in the fundus optical coherence tomography image and acquiring a boundary of the blood vessel shadow position based on the pigment epithelium gray scale curve. And determining the position of a seed point and the size of a subarea by combining an important layer boundary and a vessel shadow boundary, and assisting digital image correlation to realize retina full-field deformation measurement. According to the method, through deep fusion of deep learning and an image processing technology, the problem of automatic selection of the seed points in the fundus OCT image is solved, the efficiency, precision and reliability of retinal deformation measurement are remarkably improved, and a powerful technical tool is provided for mechanism research and clinical diagnosis and treatment of fundus diseases.

The invention relates to the technical field of biomedical detection, in particular to a urinary tract epithelium cancer detection marker composition based on ONECUT2 and DMRTA2 gene methylation, which comprises a CpG island region of an ONECUT2 gene, a CpG island region of a DMRTA2 gene and a kit for detecting urinary tract epithelium cancer. The CpG island region of the ONECUT2 gene is located in chr18: 57441548-57441674, and the CpG island region of the ONECUT2 gene is located in The CpG island region of the DMRTA2 gene is located in chr1: 50418714-50418823, and the CpG island region of the DMRTA2 gene is located in chr1: The kit for detecting urothelial carcinoma comprises a first primer pair and a first probe which are used for specifically detecting the methylation state of the CpG island region of the ONECUT2 gene in the claim 1, and a second primer pair and a second probe which are used for specifically detecting the methylation state of the CpG island region of the DMRTA2 gene. The urinary tract epithelium cancer detection marker composition based on ONECUT2 and DMRTA2 gene methylation has high sensitivity and high specificity, the sensitivity and the specificity are both 90% or above, early-stage and low-grade urinary tract epithelium cancer can be effectively detected, comprehensive coverage is achieved, and the urinary tract epithelium cancer detection marker composition is not only suitable for bladder cancer, but also suitable for upper urinary tract epithelium cancer such as renal pelvis cancer and ureter cancer.

The invention provides a colposcope collection image comparison method and system based on artificial intelligence, and the method comprises the steps: carrying out the mixed noise reduction and multi-scale enhancement processing of a 4K original image collected by a colposcope, and obtaining an enhanced noise reduction image; adaptively adjusting the scaling of the enhanced and denoised image based on image feature distribution, and focusing columnar epithelium and squamous epithelium cell features by using an attention convolutional network to obtain a feature focusing image; cross-modal feature fusion segmentation processing is carried out on the feature focusing image, columnar epithelium and squamous epithelium regions are segmented, and segmented region images are obtained; image convolution and topological feature extraction are carried out on the segmented region image, an image adjacent image is constructed, and an image feature vector is obtained; and performing fuzzy similarity measurement and comparison decision processing on the image feature vector to obtain a colposcope acquisition image comparison result. According to the invention, the defect that the consistency and the accuracy are difficult to guarantee when the colposcope collection image is identified manually at present is overcome.

The invention relates to a gingival crevicular fluid collection device and method and a manufacturing method of a sampling probe, and belongs to the field of gingival crevicular fluid collection. The gingival crevicular fluid collection device comprises the sampling probe, and the sampling probe comprises a needle body matched with the extension form of a gingival crevicular bottom; the sampling test paper covers the needle body; when the sampling probe enters the gingival sulcus bottom, the sampling test paper absorbs liquid in the gingival sulcus bottom. According to the invention, the sampling probe fitting the trend of the gingival sulcus is adopted, and the range of the probe extends from the proximal middle to the distal middle of the cheek (tongue) side, so that an operator can simultaneously collect a plurality of sites, the gingival sulcus epithelium is not easy to puncture, non-specific exudation is reduced, and the accuracy and efficiency of gingival sulcus fluid collection are improved.

The invention discloses an application of a small molecular compound SR9009 in preparation of antihypertensive drugs. The SR9009 is used for treating three common hypertension rodent animal models, a better antihypertensive effect is shown, the antihypertensive effect is possibly related to increase of urine sodium excretion and reduction of kidney epithelium sodium channel protein expression, possibility is provided for application of the SR9009 in prevention and treatment of hypertension diseases, and the SR9009 has a wide application prospect.

Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.

The present disclosure provides LNPs comprising payload molecules, eg, mRNA therapeutics, for the treatment of diseases or disorders that would benefit from delivery of the payload molecule to airway cells.

The invention relates to the technical field of biomedicine, and particularly provides a mouse model capable of being inductively combined with epithelial cell specific ablation and application of the mouse model. The method comprises the following steps: inserting a '3xFLAG-P2A-CreERT2 'functional frame at a fixed point at a termination codon of a mouse Odam gene, so as to obtain an OdamCreERT2 mouse. The expression specificity of the Odam gene in binding epithelium is verified by a mouse obtained by copulating with a ROSA26mT / mG reporter gene mouse; mice obtained by mating with ROSA26DTA cell ablation mice reveal the indispensable function of binding epithelium in maintaining periodontal barrier and preventing bone resorption. The model retains endogenous Odam expression and functions, supports protein dynamic tracing and space-time specific gene manipulation, and is suitable for researching the effect of binding epithelium in periodontal barrier integrity and alveolar bone resorption prevention.

The scalpel comprises a first scalpel handle and a first scalpel head, the first scalpel head is detachably arranged at the front end of the first scalpel handle, a connecting insertion block is arranged at the front end of the first scalpel handle, and a step-shaped clamping groove is formed in the outward end of the connecting insertion block; the tail end of the first tool bit is provided with a connecting groove matched with the stepped clamping groove and the connecting inserting block in appearance, the front end of the first tool bit is provided with an integrally-formed extending part, the extending part is bent outwards, and the width of the end is gradually reduced to form a tip in the outward extending process of the extending part. Blades are arranged on the two side surfaces of the extension part; the extension part which is bent outwards can be suitable for narrow and irregular spaces around wisdom teeth, especially at different tooth position angles, and can be conveniently inserted into a gap between a gingiva and an alveolar bone, so that the connection between the gingiva and a dental crown neck as well as between the gingiva and an alveolar is completely cut and separated, and the end part of the extension part is a sharp end; and accurate puncture and combination of the starting point of the epithelium are facilitated, and damage to healthy tissues is reduced.

The invention discloses application of rosmarinic acid and a derivative thereof as a tight connection relaxation agent. The rosmarinic acid and the derivative thereof are competitively combined with the PDZ-1 structural domain of the ZO-1 protein, connection of Claudins protein between epithelial cells is reversibly relaxed, the ability of active substances penetrating epithelial tissues of skin, small intestines and the like is safely and efficiently improved, and the rosmarinic acid and the derivative thereof can be applied to the fields of medicines, foods, cosmetics and the like.

Described herein is a delivery system and method that can be used to deliver therapeutic compositions to epithelial cells. Provided are lipid nanoparticles comprising an ionizable lipid, a sterol, a phospholipid, and a lipid conjugated to PEG. Also described are novel molecules that can be used for gene editing. Methods include methods for delivering a therapeutic cargo to the airway or epithelium of a subject and / or a method for treating an airway or gastrointestinal disorder in a subject, the method comprising administering: i) a first lipid nanoparticle comprising a surfactant; and ii) a second lipid nanoparticle comprising a therapeutic cargo. Further methods describe a method for delivering a therapeutic cargo to the airway or epithelium of a subject and / or a method for treating an airway or gastrointestinal disorder in a subject, the method comprising administering: a lipid nanoparticle comprising a therapeutic cargo, wherein the lipid nanoparticle comprises a surfactant.