130 results about "Isotopic labeling" patented technology

The invention discloses a soil microorganism carbon utilization efficiency determination method, and relates to the technical field of carbon utilization efficiency determination, and the method comprises soil sample pre-culture, multi-source 18O isotope gradient labeling, dynamic gas monitoring, microorganism respiration rate dynamic correction, and microorganism growth rate and carbon utilization efficiency acquisition. By labeling multi-source isotopes and quantifying contributions of different oxygen sources to microorganism DNA synthesis, underestimation of the carbon utilization efficiency caused by a traditional single labeling method is avoided, the problem of limitation existing in the current soil microorganism carbon utilization efficiency determination process is solved, the inhibition effect of too high local concentration on microorganism metabolism is reduced through gradient labeling, and the accuracy of soil microorganism carbon utilization efficiency determination is improved. The method has the advantages that marking uniformity is improved, multiple groups of soil samples are arranged, real-time change of microbial respiration is captured by means of dynamic monitoring, influence of isotope fractionation on carbon dioxide concentration calculation is eliminated, instantaneous errors of traditional static measurement are avoided, and accuracy of analysis results is improved to a certain extent.

The invention discloses a soil-plant carbon element tracking method and system based on isotope labeling, particularly relates to the technical field of soil carbon cycle in-situ detection, and is used for solving the technical problem that a carbon migration path in a rice rhizosphere heterogeneous micro region cannot be distinguished in the prior art. The method comprises the following steps: arranging a spatial coding microprobe array on a rice root system and a soil interface of a rice field, synchronously applying a C13 labeled compound, and continuously obtaining a grid node oxidation-reduction potential and a carbon isotope abundance value; dividing the boundary of a root surface oxidation layer and an anaerobic layer based on day and night oscillation phase difference mutation; detecting peak displacement migration from carbonyl bonds to carbon-carbon bonds in a boundary transition region by adopting an in-situ Raman spectrum, and correcting a space boundary; partitioning and clustering carbon isotope abundance values according to the corrected boundary, and identifying hot spot boundaries of a root tip secretion active region, a microorganism enrichment region and a mineral binding region; calculating the carbon isotope flux gradient in each region and generating a spatial distribution map; in-situ analysis of a rhizosphere carbon migration path is realized, and rice field carbon cycle research precision is improved.

The invention provides an electroactive biofilm for degrading enriched sulfonamide antibiotics and a method for identifying microorganisms, and relates to the technical field of environmental microorganisms and wastewater treatment. The method for degrading the electroactive biological membrane by enriching the sulfonamide antibiotics and identifying the microorganisms comprises two steps of directional enrichment of the electroactive biological membrane and identification and analysis of functional microbial communities, the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system and comprises a single carbon source stage, a common carbon source stage, a single carbon source stage and a common carbon source stage, and the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system. An isotope labeling stage; the sodium acetate in the single carbon source stage is the only carbon source; sodium acetate and sulfonamide antibiotics are used as a common carbon source in the carbon source sharing stage; in the isotope labeling stage, 13C-labeled antibiotics are used for replacing common antibiotics. According to the method, the effects of efficiently and directionally enriching, accurately identifying active degrading microorganisms and analyzing degrading genes and metabolic pathways are achieved, and technical support is provided for developing efficient degrading bacteria and bioremediation methods.

The invention discloses a kit and a method for detecting pepsin based on polypeptide, and belongs to the technical field of proteomics. The amino acid sequence of the polypeptide is as shown in SEQ ID No. 1. The polypeptide is obtained based on enzymolysis of pepsinogen PGA4 and screening, the polypeptide and isotope labeled polypeptide are utilized to establish a standard curve, a biological sample is further subjected to enzymolysis to obtain a peptide fragment sample, and pepsin in the biological sample can be quantified by detecting the content of the polypeptide in the peptide fragment sample. By utilizing the method disclosed by the invention, the pepsin in the biological sample can be rapidly quantified, so that pathological reflux and physiological reflux are diagnosed and distinguished, and the accuracy rate is high.

The invention relates to the technical field of molecular probes, and particularly discloses a molecular probe of a targeted tyrosine kinase receptor as well as a preparation method and application of the molecular probe, the molecular probe comprises radioisotope, targeted polypeptide and a chelating group, the targeted polypeptide specifically targets the tyrosine kinase receptor, the chelating group is connected with the targeted polypeptide to form a targeted precursor, and the chelating group is connected with the targeted polypeptide to form the targeted precursor. The radioactive isotope labeled precursor forms a molecular probe, and the sequence of the targeted polypeptide is shown as any one of SEQ ID No.1-8. The targeting polypeptide has good targeting property on a tyrosine kinase receptor coded by an RET gene, can realize specific high uptake in a cell line of NEPC, has relatively good biological safety, can realize accurate imaging aiming at NEPC, and clearly and durably visualizes NEPC tumors in PET imaging; meanwhile, the molecular probe can effectively delay the growth capacity of NEPC, can provide a new treatment choice for NEPC patients, and has good clinical transformation potential.

This invention relates to a method for quantifying mRNA capping efficiency, the method comprising mixing a sample, an enzyme mixture, and an isotope standard solution in a buffer solution to produce an incubation mixture, the enzyme mixture comprising a nonspecific single-stranded nuclease and an acid phosphatase, and the isotope standard comprising isotopically labeled m7G and isotopically labeled 2'-O-methylated nucleoside; incubating the mixture; and analyzing the mixture using liquid chromatography-mass spectrometry to determine at least one of capping efficiency and 2-O-methyltransferase efficiency.

The invention discloses a device and a method for testing a steam oxidation mass transfer mechanism of a metal material. The device comprises a reaction chamber, and a sample holder is arranged in the reaction chamber; the gas inlet end of the reaction chamber is simultaneously connected with an 18O2 storage piece, an inert gas storage piece, an H216O storage piece and a D218O storage piece; a first heating assembly is arranged outside the reaction chamber, a second heating assembly is arranged outside the H216O storage part, and a third heating assembly is arranged outside the D218O storage part. According to the method disclosed by the invention, an invisible ion diffusion path is converted into a detectable signal gradient through isotope labeling, and experimental verification of an oxygen ion / metal cation diffusion dominant mechanism, visual proving of a hydroxyl participation oxidation process and quantitative analysis of a dissolved oxygen effect are realized for the first time; and a direct experimental criterion is provided for optimization of the oxidation resistance of the metal material.

This invention discloses a method for the synergistic metabolic analysis of multiple components of traditional Chinese medicine (TCM) integrated with the gut microbiota. After initializing the microbiota ecosystem, a solution of multi-component TCM extracts is continuously injected into a microfluidic chip. Stable isotope-labeled precursors are pulsed into a specific simulation region to perform spatial isotope encoding. A standing wave acoustic field is applied at the sampling node of the microfluidic chip, and multiphase fluid spatial splitting is performed using the difference in the primary acoustic radiation force experienced by the particles to derive cell-free TCM metabolites. The cell-free TCM metabolites are separated by chromatography and gas phase drift separation. A four-dimensional metabolic feature array is constructed, and the absolute value of the mass shift is calculated for spatial inverse decoding. Closed-loop feedback is triggered based on the rate of change of target metabolite ion abundance, increasing the amplitude of the standing wave acoustic field pressure to confine intestinal bacteria and performing in-situ enrichment of synergistic metabolic intermediates. This invention realizes the spatial metabolic trajectory tracing and network reconstruction of multiple components of TCM in a complex gut microbiota ecosystem.

Various methods and compositions of treating 4-hydroxyphenylpyruvate dioxygenase-like (HPDL)-related diseases or disorders are presented herein. Also presented herein are methods of increasing CoQ10 biosynthesis, and methods of determining whether a subject will benefit from a CoQ10 or CoQ10 alternative treatment. Also presented herein are pharmaceutical compositions and dosage forms comprising 4-hydroxymandelic acid (4-HMA), and / or its metabolites. Further presented herein are compounds that inhibit 4-hydroxyphenylpyruvate dioxygenase-like (HPDL). Further presented herein are methods of identifying and / or assessing modulators of HPDL. Yet further presented herein are example methods and systems for isotopic labelling in cells by metabolizing cells in the presence of gaseous isotopic tracer.

The present application belongs to the technical field of analysis and identification of fermented food, and particularly relates to a method for distinguishing and identifying fermented food of different processes based on chemical isotope labeling liquid chromatography-mass spectrometry (CIL-LC-MS) technology analysis of carboxylic acid. In order to accurately and simply identify the type and content of carboxylic acid, and thus based on carboxylic acid panorama analysis, efficiently and conveniently distinguish and identify fermented food of different processes, the present application introduces CIL-LC-MS technology to analyze carboxylic acid and thus distinguish and identify fermented food of different processes. The technology can significantly enhance the response value and detection sensitivity of carboxylic acid through chemical labeling strategy, and effectively overcome the ion suppression interference of the matrix by using isotope internal standard, so as to realize accurate quantification. Combined with its inherent three-level identification process, the present application can efficiently and conveniently distinguish and identify fermented food of different processes.

The invention relates to a carbon 13 isotope labeling internal standard substance of PMMA (polymethyl methacrylate) and metabolite thereof. The invention further provides a corresponding preparation method and application. The invention fills the technical blank of PMMA / PMA exclusive isotope internal standard, promotes the mental active substance detection to develop towards the direction of higher sensitivity, lower error and stronger judicial credibility through the precise design and application of the carbon 13 labeled standard substance, and has great social value for public safety and health management.

The invention belongs to the technical field of electrochemical synthesis and isotope labeling, and particularly discloses a method for preparing a deuterated compound through electrochemical coupling and a special catalyst thereof. According to the invention, a carbon-hydrogen bond halogenation-dehalogenation deuteration process is coupled, a specific noble metal nitrogen-doped carbon catalyst is screened and applied as a cathode and anode catalytic material, a brand new electrochemical system is constructed for the first time, and under the environment-friendly reaction conditions of completely no solvent and no supporting electrolyte, through electrochemical driving, the electrochemical performance of the electrochemical system is improved. The selective halogenation of carbon-hydrogen bonds of the anode and the dehalogenation deuteration reaction of the cathode are synchronously realized, the two half reactions are successfully and efficiently coupled in the same reactor, and the deuterated organic compound with high additional value is directly synthesized. According to the method, a large amount of organic solvents and electrolyte salts used in traditional organic synthesis are abandoned, the environmental load and post-treatment difficulty of the reaction are remarkably reduced, and the method has the outstanding advantages of being simple in process, environmentally friendly and high in atom economy.

A device for synthesizing a radioisotope-labelled target tracer includes a microfluidic chip having an SCX module configured to concentrate and capture a radioisotope from a radioisotope solution and release the captured radioisotope therefrom, an SAX module configured to purify the released radioisotope from the SCX module, and an passive in-plane mixing / reaction module configured to mix the purified radioisotope with a target precursor and perform labelling reaction to synthesize the radioisotope-labelled target tracer therein. The device also includes a heating means positioned in relation to the microfluidic chip for heating the microfluidic chip during the labelling reaction; and a first valve fluidically coupled with the SCX and SAX modules and a second valve fluidically coupled with the SAX module and the in-plane mixing / reaction module for operably controlling transit of various substances or mixtures among the SCX module, the SAX modules and the in-plane mixing / reaction module.

The invention discloses imidazopyridine derivatives and application thereof, a pharmaceutical composition and a pharmaceutical preparation, and belongs to the technical field of medical chemistry. The technical problems to be solved are that the existing P2X3 receptor inhibitor is poor in safety, low in P2X3 or P2X2 / 3 selectivity, poor in metabolic stability and the like. According to the key points of the technical scheme, the imidazopyridine derivative is selected from a compound as shown in a formula I or a racemate, a stereoisomer, a geometric isomer, a tautomer, a nitrogen oxide, a hydrate, an isotope label, a solvate, a polymorphic substance, a metabolite, an ester, a pharmaceutically acceptable salt or a prodrug thereof.

The invention belongs to the technical field of hydrogel materials, and particularly relates to self-adjusting light-curing ice-cold hydrogel as well as a preparation method and application thereof.The self-adjusting light-curing ice-cold hydrogel is prepared from the following raw materials in parts by weight: 10-17 parts of a cool-feeling matrix, 18-33 parts of acrylamide 13C3, 27-42 parts of glycerol, 5 parts of TPGDA, 0.05-0.15 part of a cross-linking agent and 0.1-0.3 part of a photoinitiator, by constructing a synergistic system of antifreeze protein and a konjac glucomannan skeleton, the purpose of gelation and cool sense self-adjustment of an isotope labeling monomer is achieved, and the isotopic labeling monomer can be used for preparing drug-loaded gel dressings, cooling gel pads, cooling gel bags, cooling gel injection products and in-situ injection gel products. The photocuring cross-linking technology is beneficial to rapid forming of the gel dressing, and the stability of the effective components is improved.

The application provides a device system and a method for preparing isotopically labeled carbon dioxide, and the preparation method comprises the following steps: vaporizing heavy oxygen water, mixing the heavy oxygen water with carbon dioxide, catalyzing through a catalytic material, carrying out oxygen exchange reaction between the heavy oxygen water and the carbon dioxide, carrying out gas-liquid separation after the reaction, and obtaining isotopically labeled carbon dioxide. The device system provided by the application has the advantages of simple structure, wide source and low price of heavy oxygen water as an oxygen isotope source, oxygen exchange, replacement of oxygen-16 of conventional carbon dioxide by oxygen-18 of the heavy oxygen water, oxygen-18 isotopically labeled carbon dioxide products, simple process, green and pollution-free, high oxygen isotope utilization rate, mild separation and purification conditions, good economic benefits and application prospect.

The invention discloses an efficient preparation method of perdeuterated 3-methyl-3-butene-1-alcohol. The efficient preparation method comprises the following steps: S1, preparing deuterated 3-methyl-2-ethyl crotonate; s2, carrying out isomerization treatment on the deuterated 3-methyl-2-ethyl crotonate, and introducing a deuterium atom by taking deuterium water as a deuterium source, so as to obtain the deuterated 3-methyl-3-ethyl crotonate; and S3, under the water-free and oxygen-free conditions, carrying out reduction treatment on the deuterated 3-methyl-3-ethyl crotonate by adopting a deuterated reducing agent, so as to obtain the perdeuterated 3-methyl-3-butene-1-ol. According to the method, the perdeuterated 3-methyl-3-butene-1-ol can be prepared easily, mildly and efficiently, and technical support is provided for isotope labeling research of a terpenoid biosynthesis mechanism.

The invention discloses a compound as shown in a formula (I-A) for treating cancer, and an isotope label, an enantiomer, a diastereoisomer, an atropisomer or a pharmaceutically acceptable salt thereof. The compound provided by the invention has better inhibitory activity on WRN and has a good application prospect.

This present application discloses a pyrrolo [2, 3-b] pyridine derivative represented by formula (I), or an isotope labeled compound thereof, or an optical isomer, a geometric isomer, a tautomer, or a mixture of isomers thereof, or a pharmaceutically acceptable salt, or a prodrug thereof, or a metabolite thereof, as well as preparation method therefor, use as RET kinase inhibitor, and pharmaceutical composition comprising such derivative. Experimental results demonstrate that the compound according to the present invention can more effectively inhibit the activity of RET and its mutants compared to prior art drugs.

The present invention relates to the technical field of isotope tracing technology and discloses a method for field 13 A convenient device and method for C isotope labeling, wherein the convenient device for labeling includes a cubic bracket, which is assembled from multiple horizontal tubes and multiple vertical tubes, and is covered with a plastic film. The cubic bracket and the plastic film are assembled into an isotope labeling box, the interior of the cubic bracket is connected, and an air inlet is opened on the wall of one of the vertical tubes, and a pressure edge assembly, which includes an internal pressure edge frame and an external pressure edge frame, the external pressure edge frame adopts a splicing design, and the internal pressure edge frame is sleeved on the cubic bracket. 13 The convenient device and method for C isotope labeling can be flexibly disassembled to form a labeling box to meet the transportation and movement needs in scenarios such as changing sample sites in the field. The size of the labeling box can also be flexibly adjusted in the field scene, making it suitable for in situ labeling experiments in various field ecosystems.

The present application provides a kind of as shown in formula (I) compound or its pharmaceutically acceptable salt, stereoisomer, solvate, hydrate or isotopically labeled material, and the present application also provides the preparation method of the compound or its pharmaceutically acceptable salt, stereoisomer, solvate, hydrate or isotopically labeled material and the purposes for preparing ferroptosis inhibitor,

The invention discloses a nitrogen migration and transformation simulation and data analysis device and method, and relates to the technical field of ecological environment simulation. An isotope storage labeling module in the device is used for adding an isotope labeling substance to an environment simulator and recording basic information of the isotope labeling substance; the environment simulator is used for containing a sample substance, adding a water sample and an isotope labeling substance, and simulating the hydrological environment of the sample substance by controlling the flow of the added water sample; the liquid substance monitoring module is used for acquiring concentration profiles of dissolved substances at the overlying water-gas interface and the overlying water-sediment interface; the gas flux monitoring module is used for measuring the concentration of the gas at the overlying water-gas interface and measuring a mass-to-charge ratio signal of the gas above the overlying water-gas interface; the processing unit is used for calculating the nitrogen migration and transformation information, the nitrogen generation rate and the nitrogen removal efficiency, and the accuracy of the quantitative analysis result of the nitrogen migration and transformation behavior can be improved.

The invention relates to a compound of the formula Xm—(Y-L-T)n and a method for the detection or prognosis of a disease in the exhaled breath of a subject. X, when present is a moiety operable to target the compound to a site of interest in vivo; m is (or at least 1, Y is a cleavable group; L is a self-immolative linker, T is a terminating moiety, and n is 1 when m is 0 or n is at least 1 when m is at least 1, L is a self-immolative linker. The terminating moiety, T, may comprise an isotopically labelled reporter molecule or a precursor thereof. The reporter molecule is operable to be released upon cleavage of the cleavable group, Y; and the reporter molecule, upon release, is a volatile compound.

The invention discloses a piperidinedione compound as well as a preparation method and application thereof. The invention provides compounds as shown in formulas (I) and (II), or pharmaceutically acceptable salts, esters, stereoisomers, tautomers, polymorphs, solvates, isotope labels, metabolites or prodrugs thereof. The compound provided by the invention can be used for degrading proteins such as GSPTl and the like, so that diseases related to protein imbalance are prevented, relieved or treated; meanwhile, the compound provided by the invention can also be used as an effective load of a ligand drug conjugate, so that the adaptation disease range of the compound provided by the invention is further expanded.

The invention relates to a C13 isotope labeling internal standard substance of MAMP and metabolite thereof. The invention further provides a corresponding preparation method and application. The invention fills the technical blank of MAMP / AMP exclusive isotope internal standard, promotes the development of mental active substance detection towards the direction of higher sensitivity, lower error and stronger judicial credibility through the precise design and application of the C13 labeled standard substance, and has great social value for public safety and health management.

The invention discloses a method for detecting the carbon utilization efficiency of soil microorganisms based on fermentation bed padding, and particularly relates to the technical field of soil utilization efficiency detection.The method comprises the steps that a target fermentation bed padding sample and a corresponding applied soil sample are obtained; measuring a target fermentation bed padding sample and an applied soil sample, and constructing a characteristic parameter set; determining the microbial carbon utilization efficiency corresponding to the applied soil sample by adopting an isotope labeling method; training the input variables and the output variables by using a machine learning algorithm, and establishing a microbial carbon utilization efficiency prediction model; and predicting the carbon utilization efficiency of the to-be-detected target fermentation bed padding. The soil microorganism carbon utilization efficiency detection method is constructed through sample data acquisition, characteristic parameter determination, carbon utilization efficiency reference value determination, prediction model construction and training and carbon utilization efficiency prediction, and the problems of low detection efficiency, insufficient detection pertinence and accuracy, lack of an efficient prediction system and the like are solved.

The invention relates to the technical field of semiconductor process detection, and discloses a semiconductor process detection method and system, and the method comprises the steps: carrying out the pretreatment of a semiconductor wafer, enabling the surface of the semiconductor wafer to be free of residual pollutants, and employing a chemical cleaning and drying technology to maintain the completeness of a crystal; the method comprises the following steps: selecting a plurality of isotopes which have the same chemical property as a measured target element but have obvious natural abundance difference as standard samples, and uniformly doping an isotope solution with known concentration into the plurality of standard samples; according to the method, target element isotope labels with known concentrations are introduced into a sample, then the SIMS is used for detecting the relative signal intensity of natural isotopes and doped isotopes at the same time, so that internal correction is achieved, the ionization efficiency difference caused by different matrixes is counteracted, and due to the fact that the chemical behaviors of different isotopes in the same matrix are basically consistent, the ionization efficiency is greatly improved. The matrix effect can be greatly reduced, so that the accuracy and repeatability of quantitative analysis are improved.

A radioactive medical isotope labeled rare-earth doped nanomaterial, a PET imaging diagnosis and treatment agent, a preparation method therefor and an application thereof. The radioactive medical isotope labeled rare-earth doped nanomaterial comprises an inner core. The structural general formula of the inner core is MxTyFx+4y:Ln / R, wherein M is an alkali metal element, and T is a transition metal element and selected from one or more of Ti, Zr and / or Hf; 1 ≤ x ≤ 7, 1 ≤ y ≤ 6; Ln is a rare earth element and stable isotope; R is a radioactive medical isotope having radioactivity and selected from one or more of 90Y, 177Lu, 153Sm, 223Ra, 111In and, 89Zr. The radioactive medical isotope labeled rare-earth ion doped nanomaterial can realize deep tissue penetration high-resolution rare earth fluorescence and global PET multimodal medical images, and is a multifunctional inorganic nano rare earth fluorescent PET imaging agent capable of being effectively degraded and removed in an organism.

Positron emission tomography and magnetic resonance imaging are among the most widely used modalities, requiring isotopic labeling of bioactive molecules to generate the desired imaging probes. This method was applied to a wide range of aliphatic carboxylic acids, including complex and functionalized drug molecules, amino acids and short peptides. For non-stabilized radicals derived from benzylic and non-benzylic carboxylic acids, a copper co-catalyst was introduced to obtain the labeled nitriles, whereas metal-free conditions were applied to nitrogen-stabilized radical derived from a-amino acids and peptides to form the radiolabeled a-amino nitriles. These radiolabeled nitriles were then easily converted back to radiolabeled carboxylic acids.

The application discloses a preparation method of a human insulin purity standard substance, which comprises the following steps of raw material selection, qualitative identification, standard substance uniformity, stability test, value determination and uncertainty evaluation. The application uses commercially available high-purity human insulin as a raw material, and after purity analysis, qualitative identification, uniformity test and stability test, isotope-labeled Val, Leu and Phe are used as internal standards, and liquid chromatography-isotope dilution mass spectrometry is used for value determination, so that the interference and influence of possible isoleucine substitution leucine impurities, isomer impurities or other molecular weight protein impurities on the value determination result can be effectively excluded, and the value determination result is more accurate and reliable. The purity standard substance prepared by the application can be used for serum insulin standard substance quality value tracing, quality control of insulin measurement results in in-vitro diagnosis, related method confirmation and evaluation, and evaluation of repeatability, linearity and cross contamination rate of a closed light-emitting immune analyzer.