65 results about "Isotopic labeling" patented technology

The invention provides an electroactive biofilm for degrading enriched sulfonamide antibiotics and a method for identifying microorganisms, and relates to the technical field of environmental microorganisms and wastewater treatment. The method for degrading the electroactive biological membrane by enriching the sulfonamide antibiotics and identifying the microorganisms comprises two steps of directional enrichment of the electroactive biological membrane and identification and analysis of functional microbial communities, the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system and comprises a single carbon source stage, a common carbon source stage, a single carbon source stage and a common carbon source stage, and the directional enrichment of the electroactive biological membrane is carried out in a double-chamber bioelectrocatalysis system. An isotope labeling stage; the sodium acetate in the single carbon source stage is the only carbon source; sodium acetate and sulfonamide antibiotics are used as a common carbon source in the carbon source sharing stage; in the isotope labeling stage, 13C-labeled antibiotics are used for replacing common antibiotics. According to the method, the effects of efficiently and directionally enriching, accurately identifying active degrading microorganisms and analyzing degrading genes and metabolic pathways are achieved, and technical support is provided for developing efficient degrading bacteria and bioremediation methods.

This invention relates to a method for quantifying mRNA capping efficiency, the method comprising mixing a sample, an enzyme mixture, and an isotope standard solution in a buffer solution to produce an incubation mixture, the enzyme mixture comprising a nonspecific single-stranded nuclease and an acid phosphatase, and the isotope standard comprising isotopically labeled m7G and isotopically labeled 2'-O-methylated nucleoside; incubating the mixture; and analyzing the mixture using liquid chromatography-mass spectrometry to determine at least one of capping efficiency and 2-O-methyltransferase efficiency.

This invention discloses a method for the synergistic metabolic analysis of multiple components of traditional Chinese medicine (TCM) integrated with the gut microbiota. After initializing the microbiota ecosystem, a solution of multi-component TCM extracts is continuously injected into a microfluidic chip. Stable isotope-labeled precursors are pulsed into a specific simulation region to perform spatial isotope encoding. A standing wave acoustic field is applied at the sampling node of the microfluidic chip, and multiphase fluid spatial splitting is performed using the difference in the primary acoustic radiation force experienced by the particles to derive cell-free TCM metabolites. The cell-free TCM metabolites are separated by chromatography and gas phase drift separation. A four-dimensional metabolic feature array is constructed, and the absolute value of the mass shift is calculated for spatial inverse decoding. Closed-loop feedback is triggered based on the rate of change of target metabolite ion abundance, increasing the amplitude of the standing wave acoustic field pressure to confine intestinal bacteria and performing in-situ enrichment of synergistic metabolic intermediates. This invention realizes the spatial metabolic trajectory tracing and network reconstruction of multiple components of TCM in a complex gut microbiota ecosystem.

Various methods and compositions of treating 4-hydroxyphenylpyruvate dioxygenase-like (HPDL)-related diseases or disorders are presented herein. Also presented herein are methods of increasing CoQ10 biosynthesis, and methods of determining whether a subject will benefit from a CoQ10 or CoQ10 alternative treatment. Also presented herein are pharmaceutical compositions and dosage forms comprising 4-hydroxymandelic acid (4-HMA), and / or its metabolites. Further presented herein are compounds that inhibit 4-hydroxyphenylpyruvate dioxygenase-like (HPDL). Further presented herein are methods of identifying and / or assessing modulators of HPDL. Yet further presented herein are example methods and systems for isotopic labelling in cells by metabolizing cells in the presence of gaseous isotopic tracer.

The present application belongs to the technical field of analysis and identification of fermented food, and particularly relates to a method for distinguishing and identifying fermented food of different processes based on chemical isotope labeling liquid chromatography-mass spectrometry (CIL-LC-MS) technology analysis of carboxylic acid. In order to accurately and simply identify the type and content of carboxylic acid, and thus based on carboxylic acid panorama analysis, efficiently and conveniently distinguish and identify fermented food of different processes, the present application introduces CIL-LC-MS technology to analyze carboxylic acid and thus distinguish and identify fermented food of different processes. The technology can significantly enhance the response value and detection sensitivity of carboxylic acid through chemical labeling strategy, and effectively overcome the ion suppression interference of the matrix by using isotope internal standard, so as to realize accurate quantification. Combined with its inherent three-level identification process, the present application can efficiently and conveniently distinguish and identify fermented food of different processes.

The invention belongs to the technical field of electrochemical synthesis and isotope labeling, and particularly discloses a method for preparing a deuterated compound through electrochemical coupling and a special catalyst thereof. According to the invention, a carbon-hydrogen bond halogenation-dehalogenation deuteration process is coupled, a specific noble metal nitrogen-doped carbon catalyst is screened and applied as a cathode and anode catalytic material, a brand new electrochemical system is constructed for the first time, and under the environment-friendly reaction conditions of completely no solvent and no supporting electrolyte, through electrochemical driving, the electrochemical performance of the electrochemical system is improved. The selective halogenation of carbon-hydrogen bonds of the anode and the dehalogenation deuteration reaction of the cathode are synchronously realized, the two half reactions are successfully and efficiently coupled in the same reactor, and the deuterated organic compound with high additional value is directly synthesized. According to the method, a large amount of organic solvents and electrolyte salts used in traditional organic synthesis are abandoned, the environmental load and post-treatment difficulty of the reaction are remarkably reduced, and the method has the outstanding advantages of being simple in process, environmentally friendly and high in atom economy.

A device for synthesizing a radioisotope-labelled target tracer includes a microfluidic chip having an SCX module configured to concentrate and capture a radioisotope from a radioisotope solution and release the captured radioisotope therefrom, an SAX module configured to purify the released radioisotope from the SCX module, and an passive in-plane mixing / reaction module configured to mix the purified radioisotope with a target precursor and perform labelling reaction to synthesize the radioisotope-labelled target tracer therein. The device also includes a heating means positioned in relation to the microfluidic chip for heating the microfluidic chip during the labelling reaction; and a first valve fluidically coupled with the SCX and SAX modules and a second valve fluidically coupled with the SAX module and the in-plane mixing / reaction module for operably controlling transit of various substances or mixtures among the SCX module, the SAX modules and the in-plane mixing / reaction module.

The invention discloses imidazopyridine derivatives and application thereof, a pharmaceutical composition and a pharmaceutical preparation, and belongs to the technical field of medical chemistry. The technical problems to be solved are that the existing P2X3 receptor inhibitor is poor in safety, low in P2X3 or P2X2 / 3 selectivity, poor in metabolic stability and the like. According to the key points of the technical scheme, the imidazopyridine derivative is selected from a compound as shown in a formula I or a racemate, a stereoisomer, a geometric isomer, a tautomer, a nitrogen oxide, a hydrate, an isotope label, a solvate, a polymorphic substance, a metabolite, an ester, a pharmaceutically acceptable salt or a prodrug thereof.

The invention discloses an efficient preparation method of perdeuterated 3-methyl-3-butene-1-alcohol. The efficient preparation method comprises the following steps: S1, preparing deuterated 3-methyl-2-ethyl crotonate; s2, carrying out isomerization treatment on the deuterated 3-methyl-2-ethyl crotonate, and introducing a deuterium atom by taking deuterium water as a deuterium source, so as to obtain the deuterated 3-methyl-3-ethyl crotonate; and S3, under the water-free and oxygen-free conditions, carrying out reduction treatment on the deuterated 3-methyl-3-ethyl crotonate by adopting a deuterated reducing agent, so as to obtain the perdeuterated 3-methyl-3-butene-1-ol. According to the method, the perdeuterated 3-methyl-3-butene-1-ol can be prepared easily, mildly and efficiently, and technical support is provided for isotope labeling research of a terpenoid biosynthesis mechanism.

The present application provides a kind of as shown in formula (I) compound or its pharmaceutically acceptable salt, stereoisomer, solvate, hydrate or isotopically labeled material, and the present application also provides the preparation method of the compound or its pharmaceutically acceptable salt, stereoisomer, solvate, hydrate or isotopically labeled material and the purposes for preparing ferroptosis inhibitor,

The invention discloses a nitrogen migration and transformation simulation and data analysis device and method, and relates to the technical field of ecological environment simulation. An isotope storage labeling module in the device is used for adding an isotope labeling substance to an environment simulator and recording basic information of the isotope labeling substance; the environment simulator is used for containing a sample substance, adding a water sample and an isotope labeling substance, and simulating the hydrological environment of the sample substance by controlling the flow of the added water sample; the liquid substance monitoring module is used for acquiring concentration profiles of dissolved substances at the overlying water-gas interface and the overlying water-sediment interface; the gas flux monitoring module is used for measuring the concentration of the gas at the overlying water-gas interface and measuring a mass-to-charge ratio signal of the gas above the overlying water-gas interface; the processing unit is used for calculating the nitrogen migration and transformation information, the nitrogen generation rate and the nitrogen removal efficiency, and the accuracy of the quantitative analysis result of the nitrogen migration and transformation behavior can be improved.

The invention relates to a compound of the formula Xm—(Y-L-T)n and a method for the detection or prognosis of a disease in the exhaled breath of a subject. X, when present is a moiety operable to target the compound to a site of interest in vivo; m is (or at least 1, Y is a cleavable group; L is a self-immolative linker, T is a terminating moiety, and n is 1 when m is 0 or n is at least 1 when m is at least 1, L is a self-immolative linker. The terminating moiety, T, may comprise an isotopically labelled reporter molecule or a precursor thereof. The reporter molecule is operable to be released upon cleavage of the cleavable group, Y; and the reporter molecule, upon release, is a volatile compound.

The invention discloses a piperidinedione compound as well as a preparation method and application thereof. The invention provides compounds as shown in formulas (I) and (II), or pharmaceutically acceptable salts, esters, stereoisomers, tautomers, polymorphs, solvates, isotope labels, metabolites or prodrugs thereof. The compound provided by the invention can be used for degrading proteins such as GSPTl and the like, so that diseases related to protein imbalance are prevented, relieved or treated; meanwhile, the compound provided by the invention can also be used as an effective load of a ligand drug conjugate, so that the adaptation disease range of the compound provided by the invention is further expanded.

The invention discloses a method for detecting the carbon utilization efficiency of soil microorganisms based on fermentation bed padding, and particularly relates to the technical field of soil utilization efficiency detection.The method comprises the steps that a target fermentation bed padding sample and a corresponding applied soil sample are obtained; measuring a target fermentation bed padding sample and an applied soil sample, and constructing a characteristic parameter set; determining the microbial carbon utilization efficiency corresponding to the applied soil sample by adopting an isotope labeling method; training the input variables and the output variables by using a machine learning algorithm, and establishing a microbial carbon utilization efficiency prediction model; and predicting the carbon utilization efficiency of the to-be-detected target fermentation bed padding. The soil microorganism carbon utilization efficiency detection method is constructed through sample data acquisition, characteristic parameter determination, carbon utilization efficiency reference value determination, prediction model construction and training and carbon utilization efficiency prediction, and the problems of low detection efficiency, insufficient detection pertinence and accuracy, lack of an efficient prediction system and the like are solved.

The invention relates to the technical field of semiconductor process detection, and discloses a semiconductor process detection method and system, and the method comprises the steps: carrying out the pretreatment of a semiconductor wafer, enabling the surface of the semiconductor wafer to be free of residual pollutants, and employing a chemical cleaning and drying technology to maintain the completeness of a crystal; the method comprises the following steps: selecting a plurality of isotopes which have the same chemical property as a measured target element but have obvious natural abundance difference as standard samples, and uniformly doping an isotope solution with known concentration into the plurality of standard samples; according to the method, target element isotope labels with known concentrations are introduced into a sample, then the SIMS is used for detecting the relative signal intensity of natural isotopes and doped isotopes at the same time, so that internal correction is achieved, the ionization efficiency difference caused by different matrixes is counteracted, and due to the fact that the chemical behaviors of different isotopes in the same matrix are basically consistent, the ionization efficiency is greatly improved. The matrix effect can be greatly reduced, so that the accuracy and repeatability of quantitative analysis are improved.

A radioactive medical isotope labeled rare-earth doped nanomaterial, a PET imaging diagnosis and treatment agent, a preparation method therefor and an application thereof. The radioactive medical isotope labeled rare-earth doped nanomaterial comprises an inner core. The structural general formula of the inner core is MxTyFx+4y:Ln / R, wherein M is an alkali metal element, and T is a transition metal element and selected from one or more of Ti, Zr and / or Hf; 1 ≤ x ≤ 7, 1 ≤ y ≤ 6; Ln is a rare earth element and stable isotope; R is a radioactive medical isotope having radioactivity and selected from one or more of 90Y, 177Lu, 153Sm, 223Ra, 111In and, 89Zr. The radioactive medical isotope labeled rare-earth ion doped nanomaterial can realize deep tissue penetration high-resolution rare earth fluorescence and global PET multimodal medical images, and is a multifunctional inorganic nano rare earth fluorescent PET imaging agent capable of being effectively degraded and removed in an organism.

The application discloses a preparation method of a human insulin purity standard substance, which comprises the following steps of raw material selection, qualitative identification, standard substance uniformity, stability test, value determination and uncertainty evaluation. The application uses commercially available high-purity human insulin as a raw material, and after purity analysis, qualitative identification, uniformity test and stability test, isotope-labeled Val, Leu and Phe are used as internal standards, and liquid chromatography-isotope dilution mass spectrometry is used for value determination, so that the interference and influence of possible isoleucine substitution leucine impurities, isomer impurities or other molecular weight protein impurities on the value determination result can be effectively excluded, and the value determination result is more accurate and reliable. The purity standard substance prepared by the application can be used for serum insulin standard substance quality value tracing, quality control of insulin measurement results in in-vitro diagnosis, related method confirmation and evaluation, and evaluation of repeatability, linearity and cross contamination rate of a closed light-emitting immune analyzer.

The invention relates to a characteristic peptide fragment group for SAA protein valuing and application of the characteristic peptide fragment group. The characteristic peptide fragment group comprises a sequence 1, a sequence 1 of an I site connected isotope, a sequence 2 and a sequence 2 of an F site connected isotope, the amino acid sequence of the sequence 1 comprises a sequence as shown in SEQ ID NO.1, and the amino acid sequence of the sequence 2 comprises a sequence as shown in SEQ ID NO.2. According to the method, the isotope labeled characteristic peptide fragment is introduced as an internal standard, so that the loss in the sample treatment process and the signal fluctuation in the mass spectrometric detection process are effectively corrected, and the influence of a matrix effect on a quantitative result is overcome, so that the quantitative accuracy of the SAA protein is remarkably improved.

Provided are a GSPT1 degrading agent (a domide molecular glue derivative) having an anti-tumor effect and an antibody-drug conjugate thereof, a stereoisomer thereof, a prodrug thereof, a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable solvate thereof, or an isotopic marker thereof, use thereof, and a preparation method therefor.

The application discloses a soil microbial carbon utilization efficiency detection method based on fermented bed litter, and particularly relates to the technical field of soil utilization efficiency detection, which comprises the following steps: obtaining target fermented bed litter samples and corresponding post-application soil samples; determining the target fermented bed litter samples and the post-application soil samples, and constructing a characteristic parameter set; determining the microbial carbon utilization efficiency of the post-application soil samples by using an isotope labeling method; training input variables and output variables by using a machine learning algorithm, establishing a microbial carbon utilization efficiency prediction model; and predicting the carbon utilization efficiency of the target fermented bed litter to be detected. Through sample data acquisition, characteristic parameter determination, carbon utilization efficiency benchmark value determination, prediction model construction and training, and carbon utilization efficiency prediction, the application constructs a soil microbial carbon utilization efficiency detection method, and solves problems such as low detection efficiency, insufficient detection pertinence and accuracy, and lack of an efficient prediction system.

The application discloses a closed-loop extraction and isotope tracing method for hot flue gas displacement of coal bed methane, which comprises the following steps: first, using CH4 combustion and explosion fracturing technology to form a crack network in the in-situ coal seam by synergistic combustion and explosion reaction impact fracturing; then, injecting hot flue gas into the combustion and explosion fractured coal seam; the hot flue gas can promote the desorption of CH4 gas in the coal body due to its high temperature; the CO2, SO2 and NO2 in the hot flue gas can replace a large amount of CH4 gas adsorbed in the in-situ coal seam due to the competitive adsorption advantage; the extracted CH4 and hot flue gas can be continuously used in a closed loop; the hot flue gas storage is realized, and the coal bed methane extraction efficiency is improved; in addition, the combustion and explosion gas and the hot flue gas are marked by specific isotopes, and the migration characteristics and interaction of the combustion and explosion gas, the hot flue gas and the extracted CH4 gas in the target deep coal seam can be analyzed and reversed; therefore, the injection parameters of the combustion and explosion gas and the hot flue gas can be dynamically regulated and controlled, and the hot flue gas storage and CH4 extraction efficiency of the deep in-situ coal seam are maximized.

Provided are an antibody-drug conjugate having an anti-tumor effect, a stereoisomer thereof, a prodrug thereof, a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable solvate thereof or an isotopically labeled substance thereof, and the use thereof and a preparation method therefor.

The application discloses a synthesis method of a stable isotope-labeled metronidazole, and belongs to the field of organic synthesis. The preparation steps are three hydrogen-deuterium exchanges: adding metronidazole and a deuterium source into a reaction container, adding a deuterium water solution of a catalyst under stirring, reducing to room temperature after reaction at 75 DEG C to 85 DEG C for 110 min to 130 min, and standing for 10 h to 15 h; adjusting pH to 6 to 7 by using a deuterated acid, and removing a solvent. Finally, recrystallization by using ethanol is performed to obtain the product. The application effectively improves the purity and abundance of the isotope-labeled metronidazole through multiple hydrogen-deuterium exchanges, ensures the quality and stability of the obtained product, and realizes high raw material utilization rate, and the obtained product has high purity and high abundance.

A method for producing oxygen isotope-labeled compounds with enriched concentrations that can be used as internal standards is provided. [Solution] A method for producing an oxygen isotope-labeled compound, in which an oxygen isotope-labeled compound is obtained by labeling a substrate with a stable oxygen isotope through the action of the enzyme in a reaction solution containing an enzyme, a substrate, and a stable oxygen isotope. The method for producing an oxygen isotope-labeled compound includes the steps of preparing a substrate solution containing the substrate and a solvent, bubbling an inert gas into the substrate solution, adding the enzyme to the substrate solution, and further bubbling a gas containing the stable oxygen isotope to obtain a reaction solution containing the enzyme, the substrate, and the stable oxygen isotope, and synthesizing the oxygen isotope-labeled compound in the reaction solution.

The invention relates to a spiro compound and application thereof in the field of medicine. Specifically, the invention discloses a compound as shown in a formula (I), an isotope labeled compound thereof, an optical isomer thereof, a tautomer thereof, or a pharmaceutically acceptable salt thereof, or a crystal form or a solvate form of the compound or the pharmaceutically acceptable salt thereof.

This invention relates to the field of carbon isotope labeling technology and discloses a plant carbon isotope labeling device, including a sealed base. A sealed labeling cylinder is fixedly mounted on the top of the sealed base, and a control module is fixedly mounted on the outer wall of the sealed labeling cylinder. Oxygen produced by plant photosynthesis is separated and extracted through an oxygen selective permeation membrane, reducing the air pressure inside the sealed cavity, preventing oxygen accumulation and pressure rise, and ensuring long-term stable operation of the device. Simultaneously, the separated oxygen is used as a gas source to fill an elastic sealing ring, causing it to expand and tightly adhere to the plant stem, effectively sealing the gap between the base and the stem. This sealing ring can automatically and flexibly expand with plant growth, ensuring good airtightness without compressing or damaging the stem, meeting the needs of long-term labeling experiments. Furthermore, a pressure relief structure automatically releases pressure when the sealing ring pressure is too high, maintaining moderate expansion and further improving the safety and reliability of the device operation.

The invention discloses an application of a secondary amine reagent in carboxylic acid analysis and / or separation. The structural formula of the secondary amine reagent is one of the following structures: (PMBA) and (d5-PMBA). The PMBA and d5-PMBA provided by the invention can be used for analysis and detection of carboxylic acid, N-(piperidine-4-ylmethyl) benzamide (PMBA) and the d5-PMBA in a stable isotope form thereof are independently or jointly used as a pair of isotope labeling reagents and are subjected to condensation reaction with carboxylic acid to obtain a derivative, the carboxylic acid can be effectively detected and separated, an easily ionized group is introduced, and the detection sensitivity is high. And the mass spectrum response in an ESI positive ion mode is greatly improved.

The application discloses a kind of for pinus massoniana seedlings 13 The application discloses a device and method for marking C isotope, and relates to the technical field of forest seedling raising.In order to solve the problem that the existing isotope marking device cannot accurately control the key environmental factors affecting the growth of seedlings, the following technical solution is proposed: it comprises a marking chamber, a gas storage chamber and a reaction chamber, the marking chamber comprises a first chamber, a second chamber and a third chamber separated from each other, the side wall of the second chamber is provided with a sensor assembly, a cooler and a humidifier, the inside of the first chamber is in a vacuum state, and CO2 gas is generated in the reaction chamber 13 The CO2 gas is sequentially introduced into the gas storage chamber and the marking chamber for gas storage and marking reaction; the pinus massoniana seedlings to be marked are arranged on the multi-well plate of the second chamber, and the third chamber is provided with an alkaline solution. The sensor assembly, the cooler and the humidifier are respectively communicatively connected with an automatic control system. The application can make the marking environment more controllable and more beneficial to the normal growth of the pinus massoniana seedlings, thereby improving the marking efficiency.

A method for synthesizing a stable isotope-labeled toltrazuril-D3 belongs to the field of organic synthesis technology. This method uses methylamine hydrochloride-D3 as the stable isotope labeling source, reacting it with p-trifluoromethylthiophenol and 1-chloro-2-methyl-5-nitrobenzene as starting materials, undergoing sequential condensation and reduction to obtain an aminodiphenyl ether intermediate. Simultaneously, methylamine hydrochloride-D3 reacts with sodium cyanate to generate methylurea-D3, which then reacts with carbonyl diimidazole to obtain an activated intermediate. Finally, the aminodiphenyl ether intermediate condenses with the activated intermediate, followed by base-catalyzed cyclization to obtain the target product, toltrazuril-D3. This invention features a rationally designed synthetic route, readily available raw materials, mild reaction conditions, simple operation, and easy product separation and purification. The obtained product achieves a chemical purity and isotope abundance of over 99%.

The invention relates to the technical field of organic synthesis, in particular to boron-10 isotope labeled borate compounds and a synthesis method thereof. According to the method, boron trifluoride-10 and aryl iodide or aryl bromide are used as raw materials for the first time, and boron-10-labeled boric acid ester and derivatives thereof are efficiently synthesized in the presence of a transition metal nickel catalyst and a reducing agent zinc. Boron trifluoride-10 is used as a specific boron-10 source and is an ideal precursor for synthesizing a target isotope compound. The method is novel in route, mild in reaction condition and high in atom economy. Compared with the prior art, the method has the characteristics of high raw material pertinence, simplicity and convenience in operation, mild conditions, suitability for green synthesis of isotope labeled compounds and the like, and a new way is provided for preparation of boron-10 labeled functional molecules.