Method used for determining plant total photosynthesis carbon assimilation capacity

A technology of photosynthetic carbon and plants, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of underestimation of productivity, lack of consideration of quantification, lack of utilization of bicarbonate ions, etc., and achieve the effect of reliable data and high accuracy of results

Active Publication Date: 2015-11-18
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for measuring the total carbon assimilation capacity of plants, so as to overcome the lack of quantification of the bicarbonate ion utilization ratio of plants from the dissolution of atmospheric car...

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  • Method used for determining plant total photosynthesis carbon assimilation capacity
  • Method used for determining plant total photosynthesis carbon assimilation capacity
  • Method used for determining plant total photosynthesis carbon assimilation capacity

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Embodiment Construction

[0061] Embodiments of the invention:

[0062] It comprises the following steps. First, the seeds of the plants are germinated indoors using plugs of the same specification in hydroponics, and the seedlings are prepared for 2 months, and the seedlings with relatively consistent growth are selected as the plant seedlings for investigation; secondly, the seedlings produced by different manufacturers are tested. Sodium bicarbonate, choose two delta 13 Sodium bicarbonate whose C value difference is greater than 10‰ is used as isotope label 1 and isotope label 2, and their values ​​are δ C1 and δ C2 , were added to the culture solution in an amount of 10mM; the δ of bicarbonate ion in the culture solution of isotope label 13 C value is δ C1,0 , δ of bicarbonate ion in isotope-labeled 2 culture solution 13 C value is δ C2,0 ; 3rd, use isotope-labeled 1 and isotope-labeled 2 culture fluids to simultaneously cultivate 2-month-old plant seedlings with consistent growth, and replace...

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Abstract

The invention discloses a method used for determining plant total photosynthesis carbon assimilation capacity. According to the method, double-way isotope labeling and a double source isotope mixed module are adopted to determine using ratio of plant on added bicarbonate ions and using ratio of plant on bicarbonate ions come from atmospheric carbon dioxide dissolving; a photosynthesis device is adopted to determine photosynthesis assimilation capacity of plant on atmospheric carbon dioxide; and plant total photosynthesis carbon assimilation capacity is obtained finally. The method is used for measuring using capacity of plant on added bicarbonate ions, and measuring using capacity of plant on bicarbonate ions come from atmospheric carbon dioxide dissolving; and influences of old carbon on determination results are avoided; so that obtained plant total photosynthesis carbon assimilation capacity data is reliable, and result accuracy is high.

Description

technical field [0001] The invention relates to a method for measuring the assimilation capacity of total photosynthetic carbon of plants, which belongs to the field of ecological environment system monitoring, management and restoration. Background technique [0002] In recent years, many experiments have proved that plants can not only use carbon dioxide in the atmosphere as a substrate for photosynthesis, but also use bicarbonate ions stored in leaves from the soil as a substrate for photosynthesis. Especially in karst limestone areas with high concentrations of bicarbonate ions, the use of photosynthesis based on measuring atmospheric carbon dioxide flux to measure the inorganic carbon assimilation capacity of plants seriously underestimates the productivity of plants in karst areas. Given that the ability of plants to utilize bicarbonate ions cannot be ignored, accurate determination of the share of plant utilization of different exogenous inorganic carbon sources and t...

Claims

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Application Information

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IPC IPC(8): G01N33/00
Inventor 吴沿友杭红涛王瑞张开艳姚凯饶森陆叶赵丽华刘莹谢腾祥刘丛强王宝利
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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