Method for determining content of beta-lactoglobulin in milk powder

A technology of lactoglobulin and milk powder, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limited detection range, large error and low accuracy of radioimmunodiffusion method

A technology of lactoglobulin and milk powder, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limited detection range, large error and low accuracy of radioimmunodiffusion method

CN103995077AActive Publication Date: 2014-08-20NAT INST OF METROLOGY CHINA +1
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  • Method for determining content of beta-lactoglobulin in milk powder
  • Method for determining content of beta-lactoglobulin in milk powder
  • Method for determining content of beta-lactoglobulin in milk powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Instruments used:

[0046] Analytical balance: (METTLER TOLEDO XS205 type, the maximum weighing capacity is 81g, the precision is 0.01mg, the United States); (METTLER TOLEDO XP56 type, the maximum weighing capacity is 52g, the precision is 0.001mg, the United States);

[0047] Constant temperature shaker (S16R-2 type, SHEL LAB, USA);

[0048] Oven (UFE500 type, MEMMERT, the United States);

[0049] Liquid chromatography-mass spectrometry (TSQ Vantage Triple Quard LC / MS, Thermo, USA);

[0050] High-speed centrifuge (type 3K15, Sigma, Germany);

[0051] Ultrapure water system (Milli-Q type, MILLIPORE, USA);

[0052] Centrifugal ultrafiltration tubes (Amicon ultra-4 type, Millipore, USA).

[0053] Such as figure 1 and figure 2 Shown, a kind of method for measuring β-lactoglobulin content in milk powder, comprises the steps:

[0054] 1. The sample preparation process of β-lactoglobulin content standard substance in milk powder: divide the milk powder containing β...

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Abstract

The invention discloses a method for determining the content of beta-lactoglobulin in milk powder. The method comprises the following steps: (1) selecting TK-11 as a specific peptide fragment and conducting beta-lactoglobulin quantification; (2) synthesizing the peptide fragment, and conducting isotope labeling on the peptide fragment; (3) accurately quantifying the synthesized standard peptide fragment by adopting an isotope dilution mass spectrography; (4) conducting sample treatment and enzyme digestion; (5) hygroplasm analysis, namely filtering the solution after enzyme digestion through a filter film, and then carrying out selective ion scanning analysis on the filtrate by adopting mass spectrography; and (6) calculating according to a formula, so as to complete the determination on the content of beta-lactoglobulin in milk powder. By adopting the method, determination results are accurate and reasonable.

Description

technical field [0001] The invention relates to a method for measuring protein content, in particular to a method for measuring β-lactoglobulin content in milk powder. Background technique [0002] β-lactoglobulin (β-lactoglobulin) is a kind of protein in fresh milk, accounting for about 7-12% of fresh milk protein. The β-lactoglobulin monomer consists of 162 amino acid residues. The protein has a stable molecular structure and is not easy to be digested and hydrolyzed by pepsin. Therefore, it is very easy to join the human blood circulation through the gastrointestinal tract, and it is easy to stimulate the immune system to produce hypersensitivity reactions. It is the main allergen for infants , It is easy to cause allergies in babies (the baby's digestive system is not yet fully developed, β-lactoglobulin is easier to be absorbed "whole", and it is judged as a pathogen by the immune system). β-lactoglobulin has A, B, C3 different variants. Mainly A and B, pI between 5....

Claims

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Application Information

Patent Timeline
20 Aug 2014
Publication
CN103995077A
IPC
G01N30/88
Inventors
王洋; 武利庆