260 results about "Sample purification" patented technology

The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and / or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Fluid sample purification systems and methods are provided for isolating molecules of interest in a fluid sample. The fluid sample purification system has a housing with a distal end and distal opening adapted for the passage of a fluid and a proximal end and proximal opening adapted for passage of a fluid. A distal retainer is located inside the housing and above the distal opening. A proximal retainer is located inside the housing between the distal retainer and the proximal opening, or is located adjacent to, in contact with, or over the proximal opening. The system also comprises adsorption material, e.g., functionalized particles, inside the housing and confined between the distal retainer and the proximal retainer. The adsorption material adsorbs undesirable material while simultaneously rejecting desirable materials. Methods are also provided for isolating molecules of interest using the fluid sample purification system.

The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and / or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.

Disclosed is an automatic purification apparatus for isolating target substances from multiple biological samples, and more particularly to an automatic biological sample purification apparatus equipped with a magnetic field applying part, in which the magnetic field applying part for purifying biological samples and a heating part are integrally formed with each other so as to be movable up and down.Further, disclosed is a method of extracting a target substance from the biological sample using the automatic biological sample purification apparatus equipped with the magnetic field applying part.

The invention relates to a device for purifying and enriching inert gases and a use method. On the basis of an inert gas isotopic analysis technique, a Zr-based getter material capable of working at a room temperature is used in the invention, and meanwhile, a sample quantitative control system and a 15K low temperature cold trap are additionally arranged. All function parts are reasonably arranged and matched in the device so as to make a system have dynamic high vacuum (5.0*10-8Pa) and low leakage rate. A trace of inert gases in a single gas sample can be purified and enriched in 10 minutes. The quantitative analysis of the contents of inert gases He, Ne, Ar, Kr and Xe in the gas sample is conducted by a four-pole mass spectrometer. Meanwhile, one-step sample purification is realized, all components of the inert gases are chilled and enriched by the low temperature activated carbon cold trap (15K), and the inert gases He, Ne, Ar, Kr and Xe are gradually released in a gradual heating way. The invention lays a foundation for conducting isotopic analysis on all components of the inert gases.

The charge state of ions produced by electrospray ionization is reduced in a controlled manner to yield predominantly singly charged ions through reactions with bipolar ions generated using a <210>Po alpha particle source or equivalent. The multiply charged ions generated by the electrospray undergo charge reduction in a charge reduction chamber. The charge-reduced ions are then detected using a commercial orthogonal electrospray TOF mass spectrometer, although the charge reduction chamber can be adapted to virtually any mass analyzer. The results obtained exhibit a signal intensity drop-off with increased oligonucleotide size similar to that observed with MALDI mass spectrometry, yet with the softness of ESI and without the off-line sample purification and pre-separation required by MALDI.

The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.

The present invention describes reagents and methods for storing and / or processing of biological samples for direct use in PCR and other DNA applications. After storage, the preserved DNA in the samples may then be processed and analyzed by known methods, e.g., PCR. the present invention provides in one aspect, a method for simple and rapid storage and / or processing of nucleic acids, such as DNA, from various sources, including but not limited to body fluids, various solutions, cells, plants, tissues, bacterial cell lysates containing plasmids, etc. The present invention further comprises reagents and methods employing glycols at alkaline pH to process biological samples and make DNA usable in PCR without further sample purification. Accordingly, the present invention provides in one aspect, a method for simple and rapid processing of nucleic acids, such as DNA, from various sources, including but not limited to body fluids, various solutions, cells, plants, tissues, bacterial cell lysates containing plasmids, etc.

The invention aims at providing an immune magnetic particle for purifying aflatoxin samples and a preparing method and an application method thereof so as to solve technical problems in the prior art that aflatoxin sample purification and separation is complex in operation and low in separation efficiency and has large potential safety hazard. After aflatoxin antibodies and magnetic particles are reacted together for some time, the immune magnetic particle with specificity is prepared. After coarsely extracted aflatoxins and the immune magnetic particle are reacted together for some time, the aflatoxins are attached to the surface of the immune magnetic particle in affinity mode. After magnetic separation, the aflatoxin samples can be purified so that the relative pure aflatoxin samples can be obtained, and further the relative pure aflatoxin samples are used for fluophotometers, high performance liquid chromatographs (HPLCs) and enzyme-linked immuno sorbent assay (ELISA).

The invention relates to a sample preparation system with functions of extraction and separation of inert gas, and a use thereof. A left mechanical pump is connected to a first main pipe through a stainless steel straight pipe. A right mechanical pump is connected to a molecular pump and a low-temperature refrigerator through a quick-release four-way valve. A sample inlet is connected to the first main pipe. The first main pipe is connected to a film gauge and a first active carbon furnace. A second main pipe is connected to a high-vacuum gauge pipe, a second active carbon furnace, the molecular pump, a zirconium-based furnace and a titanium sponge furnace. A third main pipe is connected to an ion vacuum gauge, an ion pump, a getter pump, a pressure stem valve assembly, and a third active carbon furnace. A fourth main pipe is connected to the low-temperature refrigerator, an inert gas isotope mass spectrometry host machine, and a quadrupole mass spectrometer. The use of the sample preparation system with functions of extraction and separation of inert gas comprises system vacuum-pumping, sample injection control, sample purification, sample separation, and component and isotope determination. Through the sample preparation system with functions of extraction and separation of inert gas, a system vacuum degree is improved by 5 magnitudes; a rate of pollution of Ar in air on Ar in a natural gas sample is reduced to a few parts per ten thousand from a few parts per hundred; and the detection accuracy of Ar isotopes in natural gas is improved.

The invention discloses a method used for screening and identifying saxitoxins in complex matrix. The method comprises following steps: 1, preparation of a matrix sample; 2, matrix sample purification, and toxin separation; 3, establishment of a mass spectrometry database; 4, sample information acquisition and result determination; 5, high resolution mass spectrum measuring of suspected positive samples and metabolic products of the suspected positive samples; and 6, qualitative measurement used for determining accurate molecule mass. According to the method, the mass spectrometry databases of 24 kinds of saxitoxins are established, the method can be used for high precision qualitative analysis in sample screening processing, realizing blind measuring of toxic-free standard samples and accurate qualitative determination, solving problems in the prior art that standard substances are expensive, arrival period is long, and the standard substances are controlled by entry and exit administration, and reducing detection cost in daily monitoring; requirement on the technical level of operation staff in saxitoxin detection is reduced; and operation is convenient.

The invention discloses a metal-organic frameworks (MOFs) material-based series molecular sieve and a preparation method and an application method thereof in complex sample purification and separation analysis, and relates to the technical field of chromatography application and the field of solid phase micro extraction. The series molecular sieve comprises an MOFs material-based solid phase micro extraction device and an MOFs material-based capillary gas phase chromatographic column; the MOFs material-based solid phase micro extraction device comprises a section of stainless steel fiber extraction head, the surface of which is coated with an MOFs material; and the MOFs material-based capillary gas phase chromatographic column comprises a section of capillary, the inner wall of which is uniformly coated with a layer of MOFs material. High-selectivity purification, efficient separation and specificity detection and measurement of a complex sample are realized by combined use of the solid phase micro extraction device coated with the MOFs material and the gas phase chromatographic column coated with the MOFs material. The series molecular sieve is favorable for large-scale popularization and application, and has broad application prospect in the fields of analytical chemistry, particularly environmental analysis, food analysis and the like.

Fluid sample purification systems and methods are provided for isolating molecules of interest in a fluid sample. The fluid sample purification system has a housing with a distal end and distal opening adapted for the passage of a fluid and a proximal end and proximal opening adapted for passage of a fluid. A distal retainer is located inside the housing and above the distal opening. A proximal retainer is located inside the housing between the distal retainer and the proximal opening, or is located adjacent to, in contact with, or over the proximal opening. The system also comprises adsorption material, e.g., functionalized particles, inside the housing and confined between the distal retainer and the proximal retainer. The adsorption material adsorbs undesirable material while simultaneously rejecting desirable materials.; Methods are also provided for isolating molecules of interest using the fluid sample purification system.

In the present invention, we describe a device and a method that shows the use of a perforated surface (e.g. net, grid, mesh etc.) for the preparation of small biological and chemical samples. In this present invention, the sample is concentrated on the edges of the perforation. If a solution of chemical or biological sample is applied on the perforated area; upon drying the solvent, the solute will concentrate on the edges of the perforation. This offers a novel method for the sample concentration. The perforated surface can be directly placed in the MALDI instrument for sample analysis. Furthermore, the edges of the perforation of the perforated surface can be modified, treated or coated with different chromatographic materials and thus have the retention property for analyzing the molecules. The woven net may contain not just single but double or more threads that creates a capillary effect, which further enhances the sample purification and concentration.

A robotic autosampler provides for automated manipulation of microfluidic chips having multiple electrospray devices and / or sample inlets for interface to a mass spectrometer or other detection device. The autosampler also provides for connection of control voltages to the electrospray device to facilitate enablement, control and steering of charged droplets and ions. The autosampler further provides a method of fluid delivery that may be disposable or reusable. The delivery device may contain materials for component separation or sample purification. The delivery device may contain preloaded sample or the sample may be loaded by the autosampler. A method for automated manipulation of multiple electrosprays in communication with a detector, includes: providing a robot autosampler having an electrospray chip; electrospraying at least one analyte from at least one electrospray device on the electrospray chip; and manipulating the electrospray chip in communication with a detector in a manner to detect analyte from the electrospray.

An integrated cartridge for sample processing and analysis is disclosed. The integrated cartridge contains a sample preparation chamber having a sample inlet and a sample outlet, and a sample purification chamber adapted to receive a replaceable sample purification unit containing a housing and an extraction filter inside the housing. The extraction filter specifically binds to a molecule of interest. The sample purification chamber has a sample inlet that is in fluid communication with the sample outlet of the sample preparation chamber. Also disclosed is a microarray-based sample analysis (MBSA) system.

An integrated sample analysis system is disclosed. The sample analysis system contains (1) a sample preparation / analysis module having sample purification device comprising a monolith that binds specifically to nucleic acids and a sample analysis device comprising a microarray enclosed in a reaction chamber having a hydrophilic interior surface; (2) a temperature control module comprising a thermocycler having a thermally conductive temperature-control bladder; and (3) an imaging device capable of capturing an image of the microarray in the reaction chamber.

A detection method for cancer is provided. Magnetic carbon beads are used. The carbon beads are highly specified to a cancer. Surface area of grafted antigen are broadened by grafting functional molecules. Number of antigen is increased on the surface. Thus, the present invention improves sensitivity and accuracy of disease detection and greatly saves cost. The present invention can be applied for sample purification or massive disease detection.

The invention discloses a method for measuring benzo(a)pyrene in total particle phase matters in a cigarette smoke gas, which comprises the following steps of: preparing solution of D12-benzo(a)pyrene as internal standard solution, wherein the concentration of the solution is between 100 to 300 ng / mL; preparing six standard series solution of the benzo(a)pyrene; collecting the total particle phase matters in the cigarette smoke gas, and adding cyclohexane into the total particle phase matters to perform ultrasonic extraction; adding an extraction liquid into the internal standard solution of the D12-benzo(a)pyrene to perform vacuum concentration, and performing GC-MS analysis on a sample after the vacuum concentration; and performing internal standard method quantification. The method overcomes the defects that in the prior art, a non-target compound has large interference to a target compound, the sample pretreatment process is complex and the analysis time is long; and the method for measuring the benzo(a)pyrene in the total particle phase matters in the cigarette smoke gas is provided by the invention, which reduces the interference of the non-target compound to the target compound, simplifies the sample purification process, is more accurate in quantification, has a high recovery rate and good repeatability and can be widely applied in the field of tobaccos.

The invention discloses a UPLC-MS / MS method for measuring antiviral drug residuals in chicken. The method comprises the steps that a QuEChERS method is used for sample pretreatment and sample detection analysis, the sample pretreatment comprises sample extraction and purification, the sample extraction is extraction of a combination state sample in a hydrochloric acid-methanol mixture after enzymolysis under the acidic condition, and the sample purification is dispersive solid-phase extraction purification on an extracting solution by using C18 powder and PSA powder adsorbing agents after anhydrous sodium sulfate dehydration. Four types of 12 kinds of antiviral drug residuals with large compound property difference can be measured simultaneously and rapidly by improving the sample extraction and purification method and the chromatography-mass spectrometry conditions, the operation steps are simplified, the detection time is saved, the detection efficiency is improved, and the detection cost and the usage amount of the organic solvent are reduced.

The invention belongs to the technical field of material chemistry, and discloses a molecular imprinting polymer for roxarsone detection, and a preparation method thereof. The molecular imprinting polymer preparation method comprises the following steps: carrying out polymerization of a template, a pore forming agent, a functional monomer, a cross-linking agent and an initiator for 8-48 h at a temperature of 50-80 DEG C, and removing the template to obtain the molecular imprinting polymer for roxarsone detection. According to the present invention, a roxarsone recovery rate of the prepared molecular imprinting polymer is more than 90%, and the prepared molecular imprinting polymer shows a high roxarsone cross reaction, has high selectivity and high specificity, and provides broad application prospects in the field of application of the prepared molecular imprinting polymer as a sample purification pretreatment material for roxarsone analysis in feeds, animal tissues, environment water and other matrixes.

The invention relates to a melamine colorimetric detection method based on enzymatic amino hydrolysis reaction, and in particular provides a method for quantitatively detecting melamine in a sample. The method comprises the steps of: sample purification, enzymatic hydrolysis reaction, chromogenic reaction, standard curve drawing and the like. The method is simple in testing process and is rapid and easy to operate; complex special equipment and professional technicians are not needed; testing results are visual and clear, can be directly visually detected and judged, can be accurately quantified, are high in repeatability, and are not easily interfered by impurities; melamine in the sample can be effectively enriched; the high-flux measurement condition is met; and the demand of common consumers in China on simple detection of the melamine is satisfied.

The invention discloses a micro solid phase extraction probe made by an MIL-101 type metal organic framework (MOF) material and a preparation method of the probe. The probe comprises an extraction membrane bag and extraction packing. According to the membrane bag, the extraction packing is protected by adopting a hollow fiber membrane, macromolecules, particle impurities and the like can be effectively prevented from entering the membrane bag, micromolecular target compounds are adsorbed by the extraction packing, and trace organic solvents can be analyzed. The extraction packing is a MOF material MIL-101, has high specific surface area and relatively large particle size and has high adsorptive property on targets in water; the hollow fiber membrane is a polypropylene membrane, can prevent the macromolecules, particle impurities and the like and has high sensitivity, high matrix interference resistance, sample purification function and satisfactory recycling rate; moreover, the probe is simple to produce, convenient to operate and wide in substrate analysis range, can be repeatedly recycled and can be used for directly analyzing complex environmental samples.

The invention relates to a method for purifying dioxin type sample, in particular to a method for purifying sample through the following steps: based on the properties of dioxin type semi-volatile organic pollutant, combining GC separation with LC purification, introducing samples with large volume to GC through circular sampling and retention gap column; separating volatile ingredients from non-volatile ingredients through gasification and blowing; separating semi-volatile ingredients from easily volatile ingredients through GC separation, collecting the semi-volatile ingredients through stream switching; and separating the dioxin type semi-volatile organic pollutant from the semi-volatile ingredients through LC separation. Used matching equipment is composed of a high pressure pump, a sample valve, a six-way switching valve, a retention gap column, a GC analytical column, a four-way valve and an LC column. When purifying the dioxin type sample, the invention is advanced in solvent saving, simple operation and low experiment cost, and is applicable to separation and analysis of trace semi-volatile durable organic pollutant.

The invention discloses a method for detecting enrofloxacin metabolic products in a sea cucumber, belonging to the technical field of aquatic product detection. The method comprises the following steps: sample extraction; sample purification; standard curve drawing; and structural analysis of conditions of instruments used in experiments and metabolic products. According to the invention, a liquid chromatography-tandem mass spectrum-ion trap technology is applied, and a pMRM method is adopted, so that the predictable metabolic products can be accurately determined, and low-concentration metabolic products can also be detected; and by adopting the unique MRM-IDA-EPI working mode of QTrap and adopting one-step sample injection, the MRM chromatograms of the corresponding metabolic products can be obtained, and MS2 mass spectrums can also be obtained. The method can be used for detecting 10 kinds of metabolic products including ciprofloxacin, and the mass spectrum response strengths of two kinds of newly found metabolic products are far higher than that of the ciprofloxacin. By using the method, the blank that no method for detecting the enrofloxacin metabolic products in the sea cucumber is provided at present in China is made up, and a novel reference ideal is provided for researching the metabolic products of other drugs and screening unknown drugs.

The invention relates to means for extracting magnetic particles (M) from a sample (S). The sample (S) is arranged adjacent to a liquid carrier (C), which is immiscible with it, in a configuration stable under the influence of gravity, and the magnetic particles (M) are moved by a magnetic field (B) from the sample (S) into the carrier (C). Preferably, the magnetic particles (M) are non-wetting with respect to the carrier (C) and will therefore form agglomerates in the carrier (C).