260 results about "Sample purification" patented technology

Disclosed is an automatic purification apparatus for isolating target substances from multiple biological samples, and more particularly to an automatic biological sample purification apparatus equipped with a magnetic field applying part, in which the magnetic field applying part for purifying biological samples and a heating part are integrally formed with each other so as to be movable up and down.

Further, disclosed is a method of extracting a target substance from the biological sample using the automatic biological sample purification apparatus equipped with the magnetic field applying part.

The charge state of ions produced by electrospray ionization is reduced in a controlled manner to yield predominantly singly charged ions through reactions with bipolar ions generated using a <210>Po alpha particle source or equivalent. The multiply charged ions generated by the electrospray undergo charge reduction in a charge reduction chamber. The charge-reduced ions are then detected using a commercial orthogonal electrospray TOF mass spectrometer, although the charge reduction chamber can be adapted to virtually any mass analyzer. The results obtained exhibit a signal intensity drop-off with increased oligonucleotide size similar to that observed with MALDI mass spectrometry, yet with the softness of ESI and without the off-line sample purification and pre-separation required by MALDI.

The invention aims at providing an immune magnetic particle for purifying aflatoxin samples and a preparing method and an application method thereof so as to solve technical problems in the prior art that aflatoxin sample purification and separation is complex in operation and low in separation efficiency and has large potential safety hazard. After aflatoxin antibodies and magnetic particles are reacted together for some time, the immune magnetic particle with specificity is prepared. After coarsely extracted aflatoxins and the immune magnetic particle are reacted together for some time, the aflatoxins are attached to the surface of the immune magnetic particle in affinity mode. After magnetic separation, the aflatoxin samples can be purified so that the relative pure aflatoxin samples can be obtained, and further the relative pure aflatoxin samples are used for fluophotometers, high performance liquid chromatographs (HPLCs) and enzyme-linked immuno sorbent assay (ELISA).

The invention discloses a method used for screening and identifying saxitoxins in complex matrix. The method comprises following steps: 1, preparation of a matrix sample; 2, matrix sample purification, and toxin separation; 3, establishment of a mass spectrometry database; 4, sample information acquisition and result determination; 5, high resolution mass spectrum measuring of suspected positive samples and metabolic products of the suspected positive samples; and 6, qualitative measurement used for determining accurate molecule mass. According to the method, the mass spectrometry databases of 24 kinds of saxitoxins are established, the method can be used for high precision qualitative analysis in sample screening processing, realizing blind measuring of toxic-free standard samples and accurate qualitative determination, solving problems in the prior art that standard substances are expensive, arrival period is long, and the standard substances are controlled by entry and exit administration, and reducing detection cost in daily monitoring; requirement on the technical level of operation staff in saxitoxin detection is reduced; and operation is convenient.

The invention discloses a metal-organic frameworks (MOFs) material-based series molecular sieve and a preparation method and an application method thereof in complex sample purification and separation analysis, and relates to the technical field of chromatography application and the field of solid phase micro extraction. The series molecular sieve comprises an MOFs material-based solid phase micro extraction device and an MOFs material-based capillary gas phase chromatographic column; the MOFs material-based solid phase micro extraction device comprises a section of stainless steel fiber extraction head, the surface of which is coated with an MOFs material; and the MOFs material-based capillary gas phase chromatographic column comprises a section of capillary, the inner wall of which is uniformly coated with a layer of MOFs material. High-selectivity purification, efficient separation and specificity detection and measurement of a complex sample are realized by combined use of the solid phase micro extraction device coated with the MOFs material and the gas phase chromatographic column coated with the MOFs material. The series molecular sieve is favorable for large-scale popularization and application, and has broad application prospect in the fields of analytical chemistry, particularly environmental analysis, food analysis and the like.

In the present invention, we describe a device and a method that shows the use of a perforated surface (e.g. net, grid, mesh etc.) for the preparation of small biological and chemical samples. In this present invention, the sample is concentrated on the edges of the perforation. If a solution of chemical or biological sample is applied on the perforated area; upon drying the solvent, the solute will concentrate on the edges of the perforation. This offers a novel method for the sample concentration. The perforated surface can be directly placed in the MALDI instrument for sample analysis. Furthermore, the edges of the perforation of the perforated surface can be modified, treated or coated with different chromatographic materials and thus have the retention property for analyzing the molecules. The woven net may contain not just single but double or more threads that creates a capillary effect, which further enhances the sample purification and concentration.

A robotic autosampler provides for automated manipulation of microfluidic chips having multiple electrospray devices and/or sample inlets for interface to a mass spectrometer or other detection device. The autosampler also provides for connection of control voltages to the electrospray device to facilitate enablement, control and steering of charged droplets and ions. The autosampler further provides a method of fluid delivery that may be disposable or reusable. The delivery device may contain materials for component separation or sample purification. The delivery device may contain preloaded sample or the sample may be loaded by the autosampler. A method for automated manipulation of multiple electrosprays in communication with a detector, includes: providing a robot autosampler having an electrospray chip; electrospraying at least one analyte from at least one electrospray device on the electrospray chip; and manipulating the electrospray chip in communication with a detector in a manner to detect analyte from the electrospray.

An integrated cartridge for sample processing and analysis is disclosed. The integrated cartridge contains a sample preparation chamber having a sample inlet and a sample outlet, and a sample purification chamber adapted to receive a replaceable sample purification unit containing a housing and an extraction filter inside the housing. The extraction filter specifically binds to a molecule of interest. The sample purification chamber has a sample inlet that is in fluid communication with the sample outlet of the sample preparation chamber. Also disclosed is a microarray-based sample analysis (MBSA) system.

A detection method for cancer is provided. Magnetic carbon beads are used. The carbon beads are highly specified to a cancer. Surface area of grafted antigen are broadened by grafting functional molecules. Number of antigen is increased on the surface. Thus, the present invention improves sensitivity and accuracy of disease detection and greatly saves cost. The present invention can be applied for sample purification or massive disease detection.

The invention provides a soil or sediment sample pretreatment method and a dioxin determination method. A soil or sediment sample pretreatment method provided by the present invention comprises the following steps: sample extraction, sample purification, nitrogen blowing concentration. A soil or sediment sample pretreatment method and a dioxin determination method provided by the present invention innovatively combine the method of pretreatment of the soil or sediment sample with an ultrasonic and microwave combined reaction system, and purify The one-stage treatment method is simple and easy to implement, and greatly shortens the sample pretreatment time, reduces solvent consumption and cost, and has a very high promotion and application value.

The invention discloses a method for measuring benzo(a)pyrene in total particle phase matters in a cigarette smoke gas, which comprises the following steps of: preparing solution of D12-benzo(a)pyrene as internal standard solution, wherein the concentration of the solution is between 100 to 300 ng/mL; preparing six standard series solution of the benzo(a)pyrene; collecting the total particle phase matters in the cigarette smoke gas, and adding cyclohexane into the total particle phase matters to perform ultrasonic extraction; adding an extraction liquid into the internal standard solution of the D12-benzo(a)pyrene to perform vacuum concentration, and performing GC-MS analysis on a sample after the vacuum concentration; and performing internal standard method quantification. The method overcomes the defects that in the prior art, a non-target compound has large interference to a target compound, the sample pretreatment process is complex and the analysis time is long; and the method for measuring the benzo(a)pyrene in the total particle phase matters in the cigarette smoke gas is provided by the invention, which reduces the interference of the non-target compound to the target compound, simplifies the sample purification process, is more accurate in quantification, has a high recovery rate and good repeatability and can be widely applied in the field of tobaccos.

The invention belongs to the technical field of material chemistry, and discloses a molecular imprinting polymer for roxarsone detection, and a preparation method thereof. The molecular imprinting polymer preparation method comprises the following steps: carrying out polymerization of a template, a pore forming agent, a functional monomer, a cross-linking agent and an initiator for 8-48 h at a temperature of 50-80 DEG C, and removing the template to obtain the molecular imprinting polymer for roxarsone detection. According to the present invention, a roxarsone recovery rate of the prepared molecular imprinting polymer is more than 90%, and the prepared molecular imprinting polymer shows a high roxarsone cross reaction, has high selectivity and high specificity, and provides broad application prospects in the field of application of the prepared molecular imprinting polymer as a sample purification pretreatment material for roxarsone analysis in feeds, animal tissues, environment water and other matrixes.

The invention relates to a melamine colorimetric detection method based on enzymatic amino hydrolysis reaction, and in particular provides a method for quantitatively detecting melamine in a sample. The method comprises the steps of: sample purification, enzymatic hydrolysis reaction, chromogenic reaction, standard curve drawing and the like. The method is simple in testing process and is rapid and easy to operate; complex special equipment and professional technicians are not needed; testing results are visual and clear, can be directly visually detected and judged, can be accurately quantified, are high in repeatability, and are not easily interfered by impurities; melamine in the sample can be effectively enriched; the high-flux measurement condition is met; and the demand of common consumers in China on simple detection of the melamine is satisfied.

The invention discloses a micro solid phase extraction probe made by an MIL-101 type metal organic framework (MOF) material and a preparation method of the probe. The probe comprises an extraction membrane bag and extraction packing. According to the membrane bag, the extraction packing is protected by adopting a hollow fiber membrane, macromolecules, particle impurities and the like can be effectively prevented from entering the membrane bag, micromolecular target compounds are adsorbed by the extraction packing, and trace organic solvents can be analyzed. The extraction packing is a MOF material MIL-101, has high specific surface area and relatively large particle size and has high adsorptive property on targets in water; the hollow fiber membrane is a polypropylene membrane, can prevent the macromolecules, particle impurities and the like and has high sensitivity, high matrix interference resistance, sample purification function and satisfactory recycling rate; moreover, the probe is simple to produce, convenient to operate and wide in substrate analysis range, can be repeatedly recycled and can be used for directly analyzing complex environmental samples.

The invention relates to a method for purifying dioxin type sample, in particular to a method for purifying sample through the following steps: based on the properties of dioxin type semi-volatile organic pollutant, combining GC separation with LC purification, introducing samples with large volume to GC through circular sampling and retention gap column; separating volatile ingredients from non-volatile ingredients through gasification and blowing; separating semi-volatile ingredients from easily volatile ingredients through GC separation, collecting the semi-volatile ingredients through stream switching; and separating the dioxin type semi-volatile organic pollutant from the semi-volatile ingredients through LC separation. Used matching equipment is composed of a high pressure pump, a sample valve, a six-way switching valve, a retention gap column, a GC analytical column, a four-way valve and an LC column. When purifying the dioxin type sample, the invention is advanced in solvent saving, simple operation and low experiment cost, and is applicable to separation and analysis of trace semi-volatile durable organic pollutant.

The invention discloses a method for detecting enrofloxacin metabolic products in a sea cucumber, belonging to the technical field of aquatic product detection. The method comprises the following steps: sample extraction; sample purification; standard curve drawing; and structural analysis of conditions of instruments used in experiments and metabolic products. According to the invention, a liquid chromatography-tandem mass spectrum-ion trap technology is applied, and a pMRM method is adopted, so that the predictable metabolic products can be accurately determined, and low-concentration metabolic products can also be detected; and by adopting the unique MRM-IDA-EPI working mode of QTrap and adopting one-step sample injection, the MRM chromatograms of the corresponding metabolic products can be obtained, and MS2 mass spectrums can also be obtained. The method can be used for detecting 10 kinds of metabolic products including ciprofloxacin, and the mass spectrum response strengths of two kinds of newly found metabolic products are far higher than that of the ciprofloxacin. By using the method, the blank that no method for detecting the enrofloxacin metabolic products in the sea cucumber is provided at present in China is made up, and a novel reference ideal is provided for researching the metabolic products of other drugs and screening unknown drugs.

The invention relates to means for extracting magnetic particles (M) from a sample (S). The sample (S) is arranged adjacent to a liquid carrier (C), which is immiscible with it, in a configuration stable under the influence of gravity, and the magnetic particles (M) are moved by a magnetic field (B) from the sample (S) into the carrier (C). Preferably, the magnetic particles (M) are non-wetting with respect to the carrier (C) and will therefore form agglomerates in the carrier (C).