132 results about "Glyphosate" patented technology

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

The invention discloses a method for detecting glyphosate in coffee through Cu-CDs / ABEI (at) Ag nano-enzyme catalytic chemiluminescence, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that 4-aminoantipyrine serves as a precursor, Cu < 2 + > serves as a doping element, copper-doped carbon dots (Cu-CDs) are synthesized through a microwave method, Cu-CDs and ABEI serve as reducing agents to synthesize the Cu-CDs / ABEI (at) Ag nano-enzyme, and the Cu-CDs / ABEI (at) Ag nano-enzyme is used for detecting glyphosate in coffee through Cu-CDs / ABEI (at) Ag nano-enzyme. The Cu-CDs / ABEI (at) Ag nano-enzyme has excellent peroxidase (POD-like) activity, so that H2O2 decomposition is promoted, and a chemiluminescence signal is enhanced; the glyphosate can interact with the Cu-CDs / ABEI (at) Ag nano-enzyme to inhibit the POD-like activity of the nano-enzyme, so that the chemiluminescence intensity generated by oxidation of ABEI is reduced; on the basis, the method is used for detecting glyphosate in a coffee sample, and has the characteristics of high specificity, simplicity in operation and sensitivity.

The present invention relates to the fields of agriculture, plant biotechnology, and molecular biology. Specifically, the present invention relates to a wheat EPSPS mutant protein and use thereof in improving the tolerance of wheat to glyphosate. The mutant protein comprises an amino acid sequence having the following mutations compared to the EPSPS amino acid sequence of any genome of wild-type wheat: in the amino acid sequence set forth in SEQ ID NO: 2, the amino acid at position 168 is mutated from threonine to isoleucine, the amino acid at position 169 is mutated from alanine to valine, and the amino acid at position 172 is mutated from proline to serine. The mutant protein enables the production of stable glyphosate-resistant wheat, showing important significance for wheat breeding.

The present application belongs to the field of pesticides, and particularly relates to a herbicidal composition containing a fused ring substituted aromatic compound and application thereof. The composition comprises a herbicidally effective amount of active ingredient A and active ingredient B, wherein the active ingredient A is or a salt / ester thereof; the active ingredient B is selected from one or more of the following compounds and salts / esters thereof: glyphosate, glufosinate-ammonium, glufosinate-P, paraquat, diquat, ACCase inhibitors, PPO inhibitors, etc. The composition can effectively control / eliminate grass weeds and broadleaf weeds, etc., and has the characteristics of expanding the herbicidal spectrum, reducing the application amount, being able to produce a synergistic effect and solving the problem of resistant weeds, etc.

A herbicidal composition comprises an aqueous solution of a plurality of salts of glyphosate wherein said glyphosate is in anionic form accompanied by low molecular weight non-amphiphilic cations, wherein a major amount but less than 100% of the low molecular weight non-amphiphilic cations are potassium cations, and wherein the composition has a measured pH of at least about 4.8.

The invention relates to the technical field of pesticides, and discloses a glyphosate potassium salt crystal form and a preparation method thereof.The X-ray diffraction pattern of the glyphosate potassium salt crystal form comprises at least one characteristic peak in 2theta of 10.213 + / -0.2 degrees, 20.225 + / -0.2 degrees, 20.867 + / -0.2 degrees, 22.861 + / -0.2 degrees and 23.802 + / -0.2 degrees, the weed withering rate peak value of the glyphosate potassium salt 28 days after pesticide application reaches 98.48%, the weeding performance is excellent, and the glyphosate potassium salt can be applied to the field of pesticides. The crystal form has good stability, the mass change is below 0.5% after the crystal form is stored for 10 days under the conditions of humidity of 65% and room temperature, the crystal form has no obvious hygroscopicity and no obvious change, meanwhile, the crystal form has excellent performance in a long-term stability test, and the crystal form structure does not change after long-term storage.

The invention relates to the technical field of pesticides, and discloses a crystal form of ammonium glyphosate and a preparation method thereof, an X-ray diffraction pattern of the crystal form of ammonium glyphosate comprises at least one characteristic peak in 2theta of 10.196 + / -0.2 degrees, 18.339 + / -0.2 degrees, 20.207 + / -0.2 degrees, 20.857 + / -0.2 degrees, 22.483 + / -0.2 degrees, 22.845 + / -0.2 degrees, 23.416 + / -0.2 degrees, 24.868 + / -0.2 degrees, 26.811 + / -0.2 degrees, 28.878 + / -0.2 degrees or 34.057 + / -0.2 degrees, and the crystal form of ammonium glyphosate is tested to have the characteristic peak of 2theta of 10.196 + / -0.2 degrees, 18.339 + / -0.2 the crystal is regular and high in stability, the control effect reaches 96.79% after the herbicide is applied for 14 days, the control effect reaches 99.05% after the herbicide is applied for 28 days, and the weeding performance is excellent.

The invention relates to the field of herbicides, and provides soluble powder for weeding, which is prepared from the following components in parts by weight: 170 to 220 parts of triclopyr raw medicine, 40 to 70 parts of potassium hydroxide, 450 to 550 parts of glyphosate raw medicine, 250 to 350 parts of ammonium bicarbonate, 100 to 150 parts of ammonium sulfate and 60 to 120 parts of auxiliaries. The invention also provides a preparation method of the composition. The triclopyr and the glyphosate have different action mechanisms and weeding spectrums, so that the weeding range can be expanded, the weeding effect can be enhanced and the generation of drug resistance can be slowed down through the cooperative use of the triclopyr and the glyphosate; due to the conductivity of the triclopyr, the triclopyr can go deep into weed bodies, the glyphosate can be widely distributed in the weed bodies, and the triclopyr and the glyphosate are combined for use, so that respective advantages can be better exerted; the weeding soluble powder obtained by compounding the raw materials has the advantages of high efficiency, broad spectrum, rain wash resistance, freezing resistance, stability, good processability during granulation and the like, and still has the characteristic of high solubility at low temperature.

The invention relates to the technical field of glyphosate preparation, in particular to a glyphosate preparation process by a glycine method and application of the glyphosate preparation process, and the glyphosate preparation process at least comprises the following steps: carrying out dealcoholization, deacidification, crystallization, centrifugation and drying on glyphosate acidified liquid to obtain glyphosate; the crystallization step comprises the following steps: S1, transferring deacidification liquid obtained after the deacidification step to a crystallization kettle, stirring materials in the crystallization kettle, and cooling to 60-70 DEG C; s2, keeping the temperature of the material constant at 60-70 DEG C, and dropwise adding liquid caustic soda into the crystallization kettle; and S3, after the dropwise addition of the liquid caustic soda is completed, continuously stirring and cooling to 25-30 DEG C, and carrying out heat preservation to complete the crystallization step. The novel process for preparing glyphosate by using the glycine method can solve the problem that the yield and quality of a glyphosate product are affected due to incomplete deacidification in a deacidification process in a conventional process, the content of the obtained glyphosate is greater than 96%, and the yield is greater than 76%.

The application discloses a preparation method of carbon dot nanometer enzyme, which comprises the following steps: dissolving oxalic acid, polyacetyl imine and ferric nitrate in ultrapure water, heating and mixing, cooling to room temperature, centrifuging, filtering, dialyzing and taking supernatant to obtain the carbon dot nanometer enzyme; and a double-mode detection method for colorimetric and fluorescent detection of glyphosate in fruits and vegetables is constructed by using the prepared oxalic acid carbon dot nanometer enzyme, acetylcholinesterase, acetylcholine, 3,3',5,5'-tetramethyl benzidine and H2O2. The oxalic acid carbon dot nanometer enzyme is prepared by doping ferric nitrate and tetraethylenepentamine with oxalic acid as a precursor, has excellent peroxidase-like activity, and a colorimetric and fluorescent double-mode detection method for glyphosate is constructed by using the oxalic acid carbon dot nanometer enzyme, the double-mode detection results can be verified with each other, and the accuracy and reliability of the detection results are significantly improved.

Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic / transgene junctions within SEQ ID NO: 2 or with genomic / transgene junctions as set forth in SEQ ID NO: 12 and / or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

This invention discloses a glyphosate resistance-related protein, GAT_656, and its related biomaterials and applications. The protein GAT_656 is one of the following: a) a) a protein with the amino acid sequence shown in Sequence 1; b) a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of the protein shown in Sequence 1; c) a protein with the same function obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in Sequence 1; d) a protein with 75% or more identity to the amino acid sequence shown in Sequence 1 and with the same function. The protein GAT_656 and its related biomaterials of this invention can be used to breed novel glyphosate-resistant crop varieties, providing an efficient and stable solution for glyphosate resistance in crops, contributing to the improvement of agricultural production efficiency and sustainable development, and possessing significant application value.

The invention belongs to the field of gene engineering, and discloses a method for rapidly evaluating the plant growth capacity of transgenic herbicide-resistant cotton in the seedling stage, and the method comprises the following steps: S1, sowing transgenic cotton seeds to be detected; s2, when cotton cotyledons are completely flattened, glyphosate with the working concentration of 0 time or 2 times or 4 times is sprayed; s3, 7 days after spraying, respectively taking the overground part and rhizosphere soil of the cotton, and detecting the dry weight of the overground part and the water content and microbiome of the rhizosphere soil; and S4, judging the plant growth capability of the transgenic herbicide-resistant cotton in the seedling stage. The method provided by the invention is a rapid, standardized and repeatable seedling stage plant growth ability evaluation method, is suitable for multi-material and large-scale determination, is easy to popularize and apply in the agricultural field, and has urgent and important practical significance for accelerating glyphosate-resistant cotton variety breeding and industrial application.

The invention discloses an insect-resistant and herbicide-resistant transgenic maize event JZL22k-1 and a detection method of the insect-resistant and herbicide-resistant transgenic maize event JZL22k-1. The transgenic maize event JZL22k-1 nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof; the transgenic maize plant JZL22k-1 has good resistance to lepidoptera insects and good tolerance to glyphosate herbicides and has no influence on the yield, and the detection method can accurately and stably identify the existence of a JZL22k-1 transgenic event by applying a nucleic acid amplification method. The method is of great significance in implementation of research of JZL22k-1, traceability of production processing and application and whole-process supervision.

This invention discloses a continuous production equipment for solid combination herbicides, relating to the technical field of glyphosate and sulfadiazine production equipment. The continuous production equipment for solid combination herbicides includes a reactor, with a top cover fixedly connected to the top of the reactor. A first drive motor is fixedly connected to the top of the top cover, and a rotating shaft is rotatably connected inside the first drive motor. By setting a rotating collection block, a second drive motor is activated to drive spiral blades and first blades to rotate, thereby guiding agglomerated particles deposited at the bottom of the reactor through the spiral blades. Finally, the rotation of the first blades blows the agglomerated particles directly upwards and disperses them, accelerating particle dissolution. In conjunction with an extrusion block, the particles blown by the first blades pass through a rectangular box and ultimately enter the interior of an arc-shaped pipe. A corrugated plate continuously drives the extrusion block to vibrate during the rotation of the rotating shaft, further dispersing the agglomerated particles.

The invention discloses application of combination of ginsenoside Rb1 and luteolin in prevention and treatment of male reproductive toxicity caused by organophosphorus pesticides, and belongs to the field of medicines. A high-toxicity organophosphorus pesticide glyphosate is used as a representative, the reproductive toxicity effect of the glyphosate on male mice and the relieving effect of the ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin on the male reproductive toxicity of the glyphosate are observed through tests, and the result shows that the ginsenoside Rb1 and the luteolin composition have the effect of inhibiting the reproductive toxicity of the glyphosate. The ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin have a remarkable relieving effect on male reproductive toxic diseases caused by organophosphorus pesticides, wherein the male reproductive toxic diseases comprise testis diseases and sperm malformation rate increase, and antioxidant index GSH decrease and T-SOD increase caused by the organophosphorus pesticides. Effective drugs and treatment strategies are provided for preventing and treating organophosphorus pesticide induced male reproductive toxicity and diseases induced by organophosphorus pesticide induced male reproductive toxicity.

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

The present application relates to the field of genetic engineering and bioinformatics, and particularly relates to a method for obtaining glyphosate-resistant trait without fitness cost. The method comprises the following steps: generating at least two copies of endogenous epsps gene in a plant by chromosome fragment doubling, wherein at least one copy of the epsps gene is subjected to a point mutation for glyphosate resistance and at least one copy remains wild type. The plant obtained by the method has no obvious loss of agronomic traits compared with wild type plants, and has significantly improved resistance to glyphosate herbicide, and has significant application value.

The invention provides a herbicide for preventing and treating cyperus rotundus in cooperation with glyphosate. The herbicide is prepared from bacillus velezensis fermentation liquor, phenolic acid and glyphosate. The phenolic acid comprises any one or a combination of more of p-coumaric acid, cinnamic acid, p-hydroxybenzoic acid and caffeic acid. The bacillus velezensis fermentation liquor and phenolic acid components are introduced and form a synergistic effect with the glyphosate, so that the dosage of a single dosage of the glyphosate is reduced, the control effect is guaranteed, meanwhile, the potential pressure of chemical pesticide input on the ecological environment is reduced, and the environmental compatibility of a weeding system is improved.

The invention discloses a preparation method of a silicon quantum dot-Fe < 3 + > fluorescent probe and application of the silicon quantum dot-Fe < 3 + > fluorescent probe in detection of glyphosate pesticide residues, belongs to the field of analytical chemistry, and solves the problems that an existing detection method is long in detection time consumption, complex in sample pretreatment and difficult in high-throughput analysis. The preparation method comprises the following steps: introducing a newly prepared sodium ascorbate solution and a 3-aminopropyltrimethoxysilane solution into ultrapure water for reaction; dialyzing the solution in distilled water to remove unreacted solvent, and storing the obtained solution in a dark place at room temperature; carrying out rotary evaporation on the solution until the solution is viscous, pumping by a vacuum pump until the solution is dry, and dissolving by distilled water to obtain a silicon quantum dot solution; and incubating the silicon quantum dot solution and Fe < 3 + > at room temperature to form the silicon quantum dot-Fe < 3 + > fluorescent probe. The preparation method is simple, and reaction products are easy to separate and purify; the method is simple and convenient in glyphosate detection operation, good in selectivity, rapid in response and high in sensitivity, and can be used for rapid and quantitative detection of glyphosate pesticide residues in vegetables.

The invention provides an insect-resistant and herbicide-resistant gene of corn and application of the insect-resistant and herbicide-resistant gene to cultivation of insect-resistant and herbicide-resistant corn, and belongs to the field of plant disease resistance. Experiments prove that the expression vector for simultaneously expressing the Cry1Ab, Cry2Ab and cp4-epsps genes is constructed for the first time, transgenic crops obtained by transforming crops with the vector can simultaneously resist Lepidoptera pests such as ostrinia nubilalis and cotton bollworm and have good glyphosate resistance, the insect-resistant and herbicide-resistant application of the transgenic crops is widened, and the transgenic crops have broad application prospects. The method has a wide application prospect in corn genetic breeding improvement.

The application discloses a device for directional conversion of glyphosate high-salt phosphorus-containing organic waste liquid, and relates to the technical field of glyphosate wastewater treatment, comprising a directional conversion part, a high-temperature incineration part, a waste heat recovery part, a flue gas quenching part, a flue gas dust removal part, a flue gas deacidification part, a flue gas denitration part, a chimney, and a sodium pyrophosphate recovery part; the vertical constant-temperature furnace is provided with waste liquid top spraying, and a plurality of natural gas burners are arranged in layers to ensure uniform combustion temperature; the vertical constant-temperature furnace, the horizontal constant-temperature furnace and the flue constitute a U-shaped directional conversion structure, the directional conversion time is prolonged, and the conversion efficiency is improved; the secondary combustion chamber is arranged on the top of the waste heat boiler to avoid molten salt from blocking the flue; air is supplied to the bottom of the beam type chain grate to ensure that the TOC content of the recovered inorganic salt is close to zero; the application does not generate sewage and solid waste, flue gas emission meets environmental protection requirements, waste reduction, harmlessness and resource utilization are realized, the sustainable development of pollution-type industries is promoted, the competitiveness of enterprises and industries is improved, and technical progress and industry technology leap are realized.

The present disclosure relates to novel methods and compositions for conferring glyphosate tolerance to plants. The disclosure also provides glyphosate tolerant plants, seeds, tissues, cells and plant parts comprising the modified EPSP synthase and a recombinant DNA molecule encoding the modified EPSP synthase, as well as methods of producing the glyphosate tolerant plants, seeds, tissues, cells and plant parts and uses thereof.

The invention relates to a disposable electrochemical biosensor for use in the sensitive determination of glyphosate (GLY), a herbicide, and the preparation method thereof. In the electrochemical biosensor of the invention, carboxymethyl cellulose (CMC) / hydroxyapatite (HaNP) nanobiocomposite was modified onto the surface of a disposable pencil graphite electrode (PGE). DNA aptamer (DNA APT), a specific bioreceptor of glyphosate, was immobilised on the CMC / HaNP-PGE surface.

The utility model relates to the field of environment-friendly property detection devices, in particular to a glyphosate herbicide environment-friendly property detection device which comprises a hopper and a collecting pipe arranged in the hopper, the limiting assembly can enable the collecting pipe to vertically sample and is arranged in the hopper; through the arrangement of the limiting assembly, when a worker needs to collect the soil sprayed with the herbicide and carry out an environmental protection test, the sleeve and the shell can be vertically aligned only by pedaling a treading ring, so that the collection pipe can keep vertically downward displacement, and the position accuracy of soil collection and the accuracy of environmental protection performance detection data are improved; by arranging a shell, a sliding seat, an adjusting rod and an opening and closing plate in a hopper, when a sleeve vertically moves downwards, the opening and closing plate can be automatically opened, so that a collecting pipe moves downwards to collect soil, and after collection is completed, the opening and closing plate can be automatically closed through resetting of a second spring, so that the phenomenon that the soil falls off is prevented.

This invention discloses a method for the simultaneous remediation of pesticide and arsenic pollution in acidic soil, belonging to the field of soil remediation technology. *Pseudomonas arsenicoxydans* Y24-2 is used as the remediation microbial agent, and calcium peroxide is used as remediation agent A. Sodium acetate, urea, potassium dihydrogen phosphate, manganese salt, and ferrous salt are mixed to obtain remediation agent B. Remediation agents A and B must be stored separately and mixed immediately before remediation. Remediation agents A and B are dispersed in water, and the remediation microbial agent is added to obtain a remediation agent suspension. The remediation agent suspension is immediately applied to the acidic soil to be remediated for simultaneous remediation of pesticide and arsenic pollution. According to experiments, after remediation using the method provided by this invention, glyphosate in the soil is effectively degraded, arsenic is effectively oxidized and passivated, and the soil pH value is raised to neutral, achieving the goal of pollutant removal and soil restoration to health.

The application discloses a strain Aureimonas ureilytica L12 and application thereof. The application discloses a glyphosate-degradable strain L12 isolated from fresh leaves of a glyphosate-resistant male plant of longleaf goosegrass, Aureimonas ureilytica The strain L12 has a high glyphosate degradation rate. The strain L12 provided by the application enriches a germplasm resource library of pesticide-degradable bacteria, and by using the strain L12, the environmental residue of glyphosate can be significantly reduced, and the harm of glyphosate to an ecological system and human health can be reduced.

The application discloses a metal Mn-doped organic framework material for glyphosate degradation and a synthesis method thereof, and belongs to the technical field of metal organic framework materials. The synthesis method adopts MOF-808 as a metal framework material and adopts Mn(NO3)2.4H2O as a doped metal to realize green synthesis. The prepared material has the advantages of fast response time, high selectivity and high degradation rate. After the metal framework material reacts with GP, the P=O double bond in the structure of the organophosphorus pesticide glyphosate is broken, the macromolecular toxic substance is converted into a small-molecule non-toxic substance, and great potential is achieved in the aspect of environmental protection.

The application discloses a method for detecting glyphosate in coffee by Cu-CDs / ABEI@Ag nanozyme catalytic chemiluminescence, and belongs to the technical field of chemical analysis and detection. 2+ The application is characterized in that 4-aminoantipyrine is used as a precursor, Cu is used as a doping element, Cu-doped carbon dots (Cu-CDs) are synthesized by a microwave method, Cu-CDs and ABEI are used as reducing agents to synthesize Cu-CDs / ABEI@Ag nanozyme, the Cu-CDs / ABEI@Ag nanozyme has excellent peroxidase-like (POD-like) activity, promotes the decomposition of H2O2, and enhances a chemiluminescence signal; glyphosate can interact with the Cu-CDs / ABEI@Ag nanozyme to inhibit the POD-like activity of the nanozyme, so that the chemiluminescence intensity generated by the oxidation of ABEI is reduced; and based on this, the method is used for detecting glyphosate in coffee samples, and has the characteristics of high specificity, simple operation and high sensitivity.

Herbicidal compositions comprising high load aminophosphate or aminophosphonate salts, in particular high load glyphosate salts, wherein the compositions comprises a surfactant mixture of at least an C 6-C 22 amine oxide and a tertiary amine surfactants. The compositions have good low temperature stability (i.e., no crystal formulation or phase separation) among other beneficial properties. (No accompanying figure)