22 results about "Glyphosate" patented technology

This invention discloses a glyphosate resistance-related protein, GAT_656, and its related biomaterials and applications. The protein GAT_656 is one of the following: a) a) a protein with the amino acid sequence shown in Sequence 1; b) a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of the protein shown in Sequence 1; c) a protein with the same function obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in Sequence 1; d) a protein with 75% or more identity to the amino acid sequence shown in Sequence 1 and with the same function. The protein GAT_656 and its related biomaterials of this invention can be used to breed novel glyphosate-resistant crop varieties, providing an efficient and stable solution for glyphosate resistance in crops, contributing to the improvement of agricultural production efficiency and sustainable development, and possessing significant application value.

This invention discloses a continuous production equipment for solid combination herbicides, relating to the technical field of glyphosate and sulfadiazine production equipment. The continuous production equipment for solid combination herbicides includes a reactor, with a top cover fixedly connected to the top of the reactor. A first drive motor is fixedly connected to the top of the top cover, and a rotating shaft is rotatably connected inside the first drive motor. By setting a rotating collection block, a second drive motor is activated to drive spiral blades and first blades to rotate, thereby guiding agglomerated particles deposited at the bottom of the reactor through the spiral blades. Finally, the rotation of the first blades blows the agglomerated particles directly upwards and disperses them, accelerating particle dissolution. In conjunction with an extrusion block, the particles blown by the first blades pass through a rectangular box and ultimately enter the interior of an arc-shaped pipe. A corrugated plate continuously drives the extrusion block to vibrate during the rotation of the rotating shaft, further dispersing the agglomerated particles.

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

The present application relates to the field of genetic engineering and bioinformatics, and particularly relates to a method for obtaining glyphosate-resistant trait without fitness cost. The method comprises the following steps: generating at least two copies of endogenous epsps gene in a plant by chromosome fragment doubling, wherein at least one copy of the epsps gene is subjected to a point mutation for glyphosate resistance and at least one copy remains wild type. The plant obtained by the method has no obvious loss of agronomic traits compared with wild type plants, and has significantly improved resistance to glyphosate herbicide, and has significant application value.

The application discloses a device for directional conversion of glyphosate high-salt phosphorus-containing organic waste liquid, and relates to the technical field of glyphosate wastewater treatment, comprising a directional conversion part, a high-temperature incineration part, a waste heat recovery part, a flue gas quenching part, a flue gas dust removal part, a flue gas deacidification part, a flue gas denitration part, a chimney, and a sodium pyrophosphate recovery part; the vertical constant-temperature furnace is provided with waste liquid top spraying, and a plurality of natural gas burners are arranged in layers to ensure uniform combustion temperature; the vertical constant-temperature furnace, the horizontal constant-temperature furnace and the flue constitute a U-shaped directional conversion structure, the directional conversion time is prolonged, and the conversion efficiency is improved; the secondary combustion chamber is arranged on the top of the waste heat boiler to avoid molten salt from blocking the flue; air is supplied to the bottom of the beam type chain grate to ensure that the TOC content of the recovered inorganic salt is close to zero; the application does not generate sewage and solid waste, flue gas emission meets environmental protection requirements, waste reduction, harmlessness and resource utilization are realized, the sustainable development of pollution-type industries is promoted, the competitiveness of enterprises and industries is improved, and technical progress and industry technology leap are realized.

The invention relates to a disposable electrochemical biosensor for use in the sensitive determination of glyphosate (GLY), a herbicide, and the preparation method thereof. In the electrochemical biosensor of the invention, carboxymethyl cellulose (CMC) / hydroxyapatite (HaNP) nanobiocomposite was modified onto the surface of a disposable pencil graphite electrode (PGE). DNA aptamer (DNA APT), a specific bioreceptor of glyphosate, was immobilised on the CMC / HaNP-PGE surface.

This invention discloses a method for the simultaneous remediation of pesticide and arsenic pollution in acidic soil, belonging to the field of soil remediation technology. *Pseudomonas arsenicoxydans* Y24-2 is used as the remediation microbial agent, and calcium peroxide is used as remediation agent A. Sodium acetate, urea, potassium dihydrogen phosphate, manganese salt, and ferrous salt are mixed to obtain remediation agent B. Remediation agents A and B must be stored separately and mixed immediately before remediation. Remediation agents A and B are dispersed in water, and the remediation microbial agent is added to obtain a remediation agent suspension. The remediation agent suspension is immediately applied to the acidic soil to be remediated for simultaneous remediation of pesticide and arsenic pollution. According to experiments, after remediation using the method provided by this invention, glyphosate in the soil is effectively degraded, arsenic is effectively oxidized and passivated, and the soil pH value is raised to neutral, achieving the goal of pollutant removal and soil restoration to health.

The present invention relates to aqueous dispersions comprising an ALS inhibitor, typically a sulfonylurea herbicide, specifically from the class of triazolinones herbicides, a HPPD inhibitor, specifically a triketone as from the class of 2-benzoyl-1,3-cyclohexanedione herbicides, optionally a safener from the class of the sulfonamides, a water-soluble herbicide present as a salt, specifically glyphosate and to dispersions resulting from dispersing the concentrate suspension in water, to a method of making the aqueous suspension and to a method of controlling unwanted plants using these dispersions.

This invention relates to the fields of agricultural science and plant bioengineering, particularly to the application of the Osbc1 gene in glyphosate resistance. The invention transforms yeast with the Osbc1-YES2-NTB plasmid, which grows in a glyphosate-containing selection medium and exhibits glyphosate resistance. The gene regulation strategy proposed in this invention provides genetic resources for breeding herbicide-resistant crops and has significant agricultural application value.

Provided is a method for obtaining a glyphosate-resistant trait without a fitness cost. The method comprises the following steps: producing at least two copies of an endogenous epsps gene by means of in planta chromosomal segment duplication, wherein a point mutation conferring resistance to glyphosate occurs in at least one copy of the epsps gene, and at least one copy remains wild-type. Compared with a wild-type plant, the plant obtained by the method exhibits no significant loss of agronomic traits, demonstrates significantly improved resistance to glyphosate-based herbicides, and offers great application value.

The application discloses a glyphosate herbicide composition, which comprises an active ingredient glyphosate, a phosphorus-containing additive, amino acids, an auxiliary agent and other substances. The herbicide composition has a synergistic effect compared with single glyphosate herbicide and glyphosate and phosphorus-containing additive combined herbicide.

The present application provides a preparation method of glyphosate and triclopyr soluble powder, comprising the following process steps: triclopyr, inorganic base and appropriate amount of water are put into a kneader, stirring is started, the mixing temperature of the materials is 40-50 DEG C, the materials are unloaded after stirring and mixing, the materials are stored for three days, then dried and crushed. Triclopyr salt, filler are put into a mixer, glyphosate salt and adjuvant are added and mixed, then taken out and crushed. The glyphosate and triclopyr soluble powder product prepared by the present application has stable quality, excellent solubility and wetting performance, has good control effect on weeds, and the preparation method is simple in operation, lower in energy consumption and less in wastewater.

The present application relates to a glyphosate pesticide production wastewater treatment system, belonging to the wastewater treatment technical field, including the communication of the regulating pool, the demulsification reactor, the coagulation sedimentation tank, the iron carbon tower, the Fenton oxidation tank, the primary A / O tank, the secondary A / O tank, the secondary sedimentation tank, the depth phosphorus removal area and the enhanced sedimentation tank, the regulating pool is equipped with salinity control and aeration stirring device, the primary A / O tank is additionally provided with anaerobic section, the depth phosphorus removal area is arranged between the secondary sedimentation tank and the enhanced sedimentation tank, the regulating pool, coagulation sedimentation tank, iron carbon tower, Fenton oxidation tank, primary A / O tank, secondary A / O tank, secondary sedimentation tank, depth phosphorus removal area, enhanced sedimentation tank are all communicated with sludge concentration tank through pipeline, and biochemical residual sludge is backflowed to iron carbon tower and two-stage A / O tank, the present application is through demulsification enhanced pretreatment and coagulation pre-phosphorus removal, and emulsified oil, suspended solids are removed efficiently, and total phosphorus load is reduced, so that subsequent unit blockage and high load problems are avoided.

The application discloses a preparation method of a copper-iron bimetallic nano-enzyme and application thereof, and belongs to the technical field of nano-enzyme preparation and catalytic detection, and solves the technical problems of single nano-enzyme activity, low detection flux, and difficulty in simultaneously detecting multi-component pesticide residues in the prior art. The GMP, copper ions and iron ions are subjected to self-assembly reaction in a buffer solution, and after separation and drying, a GMP-CuFe bimetallic nano-enzyme is obtained. The GMP-CuFe nano-enzyme has dual activities of peroxidase and laccase, a colorimetric array sensor comprising three sensing channels of LAC incubation, LAC and POD is constructed, and by utilizing the differential colorimetric signals generated after different pesticides and the nano-enzyme act in different channels, and by combining linear discriminant analysis, the glyphosate, nicosulfuron, halosulfuron-methyl, metsulfuron-methyl and ferrous methylene bis (dimethyl dithiocarbamate) can be distinguished and quantitatively detected. The preparation method is simple and low in cost, the sensor array is high in sensitivity, good in selectivity and strong in anti-interference capability.

This invention relates to the field of agricultural biotechnology, specifically to a rice ACCase mutant gene E2079D and its applications. The invention provides a rice ACCase mutant gene E2079D, which is a mutation at position 6237 of the wild-type rice ACCase gene. Substances containing this mutant gene exhibit excellent resistance to acetyl-CoA inhibitor herbicides. When the mutant gene is transferred into wild-type rice, it confers resistance to acetyl-CoA inhibitor herbicides. Field spraying results at the three-leaf stage of rice show that mutant rice containing this mutant gene can maintain normal growth and development even with concentrations of 4 times higher than that of glyphosate, and its seed setting rate and growth period remain unaffected. In contrast, wild-type plants show chlorosis and yellowing at only 1 times the concentration, gradually withering after 5 days until the entire plant dies.

This invention discloses a near-ultraviolet emitting copper nanocluster, its preparation method, and its applications. The chemical composition of the copper nanocluster is Cu4MPD7, and its fluorescence emission peak is located near 343 nm. The preparation method employs a simple one-pot aqueous phase method, using m-phenylenediamine as a reducing agent and ligand, reacting it with copper sulfate at room temperature followed by centrifugation. This nanocluster can be used as a fluorescent probe, emitting near-ultraviolet emission through its surface Cu... 2+ The specific binding with glyphosate induces fluorescence enhancement, enabling highly sensitive and selective detection of glyphosate, which has been successfully applied to the analysis of actual samples. Furthermore, due to its near-ultraviolet emission properties and excellent biocompatibility, this nanocluster also has significant application potential in fields such as biolabeling, in vivo imaging, disease treatment, catalytic materials, and the preparation of composite nanomaterials. This invention features a simple process, low cost, and environmental friendliness, providing new materials and methods for pesticide residue detection and the development of functional nanomaterials.

Compositions, methods, systems and equipment capable of significantly reducing glyphosate, microbial, mycotoxin and other contaminant levels in various food commodities, such as, but not limited to, grains (e.g., corn, wheat, oats barley, rye, sorghum, millet, rice, etc.), nuts, seeds, and other agricultural products, are described. A contaminant treatment fluid suitable for use in reducing contaminant levels in foods may include an electrolyzed carrier fluid having a reductive potential with an oxidizer, a carbohydrate, and a sulfonic acid functional group-containing compound. Contacting food commodities with the contaminant treatment fluid reduces contaminant levels in the food commodity.

This invention discloses a nanocomposite material and its preparation method, a nanoenzyme-bioenzyme cascade sensor and its application, relating to the field of pesticide detection technology. The technical solution of this invention introduces flexible two-dimensional graphitic carbon nitride nanosheets as a soft template and spatial confinement agent for in-situ growth, effectively suppressing the excessive growth of PCN-222(Fe) crystals during the solvothermal process, successfully controlling its size from the micrometer scale to the submicrometer scale, resulting in higher catalytic activity. The nanoenzyme-bioenzyme cascade sensor constructed based on this composite material achieves broad-spectrum, ultrasensitive detection of pesticides such as glyphosate, dimethoate, and paraoxon. This sensor has a low detection limit in the pM-nM range, a wide linear range, and exhibits excellent recovery and precision in complex real-world samples, strong anti-interference ability, and high practicality.