253 results about "Glyphosate" patented technology

The invention discloses a preparation method of a carbon dot nano-enzyme, which comprises the following steps: dissolving oxalic acid, polyethyleneimine and ferric nitrate in ultrapure water, heating, mixing and reacting, cooling to room temperature, centrifuging, filtering, dialyzing, and taking supernate to obtain the carbon dot nano-enzyme. The prepared oxalic acid carbon dot nano-enzyme is matched with acetylcholin esterase, acetylcholine, 3, 3 ', 5, 5'-tetramethyl benzidine and H2O2 to construct a colorimetric and fluorescent dual-mode detection method for glyphosate in fruits and vegetables. The oxalic acid carbon dot nano-enzyme is prepared by doping ferric nitrate and tetraethylenepentamine into oxalic acid serving as a precursor, the oxalic acid carbon dot nano-enzyme has excellent peroxidase-like activity, a colorimetric and fluorescent dual-mode detection method of glyphosate is constructed through the oxalic acid carbon dot nano-enzyme, dual-mode detection results can be mutually verified, and the detection result is accurate. And the accuracy and the reliability of a detection result are remarkably improved.

The invention discloses a rice EPSPS enzyme mutant, an expression cassette, a recombinant bacterium or an application thereof, and relates to the technical field of plant genetic engineering. The invention can obtain a glyphosate-resistant rice EPSPS enzyme by mutating a wild-type rice EPSPS enzyme at a specific position, and after transforming the wild-type rice EPSPS enzyme into a plant, the rice EPSPS enzyme mutant can be expressed and has glyphosate resistance. The rice EPSPS enzyme mutant provided by the invention can effectively solve the problem of non-selective damage to crops by herbicides such as glyphosate, and provides a new approach for cultivating glyphosate-resistant crop varieties. At the same time, the rice EPSPS enzyme mutant, nucleic acid molecule, expression cassette or vector or recombinant bacterium or recombinant cell provided by the invention has broad application prospects in cultivating glyphosate-resistant rice.

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

The invention provides a V2O5-NC nano-enzyme as well as preparation and application thereof. The V2O5-NC nano-enzyme is dark green powder and has a stacked layered structure, the specific surface area is 16-18m < 2 > / g, mesoporous distribution is realized, the average particle size is 20-25nm, and the V2O5-NC nano-enzyme has catalase-like activity. The wide-interlayer-spacing V2O5-NC nano-enzyme based on C-O bond bridging prepared by the invention has relatively good stability, can be prepared on a large scale, is convenient to store, has good affinity to catalytic chromogenic substrates, has high stability, and has universality for the chromogenic reaction of the catalytic chromogenic substrates (TMB, ABTS, DAB and OPD). The V2O5-NC nano-enzyme disclosed by the invention is used for analyzing five organophosphorus pesticides (parathion-methyl, glyphosate, phoxim, methamidophos and chlorpyrifos), has good pesticide recognition capability and interference resistance, and also has the advantages of wide linear range, low detection limit, simplicity and convenience in operation, low detection cost and the like.

The present invention relates to a method for post-emergent weed control, which comprises applying an effective amount of a composition comprising herbicide A (component A), glyphosate (component B) and optionally herbicide C (component C) to emerged weeds, or an area, where weeds are growing.

The invention provides a method for physically-chemically synergistically and ecologically preventing and controlling xanthium sibiricum, and belongs to the technical field of comprehensive treatment of invasive plants. The method for physically-chemically synergistically and ecologically preventing and controlling xanthium sibiricum comprises the following steps: spraying a glyphosate solution at the seedling stage of xanthium sibiricum, and covering with a black mulching film. According to the method, the control effect on xanthium sibiricum reaches up to 97% or above, the seed bank density is reduced by 93% or above, xanthium sibiricum populations can be rapidly reduced on the basis of low-cost input, the influence on other species is small, protection of biological diversity and maintenance of ecological system stability are facilitated, and the method is suitable for large-scale ecological management.

The invention belongs to the technical field of plant breeding, and relates to a transgenic maize event LP112-1 and a detection method thereof. The invention provides a nucleic acid molecule for detecting a transgenic maize event LP112-1, the sequence of the nucleic acid molecule is as shown in SEQ ID NO: 1-2, SEQ ID NO: 3-4, SEQ ID NO: 5 or one or more groups of complementary sequences thereof, the nucleic acid molecule is derived from the transgenic maize event LP112-1, and the sequence of the nucleic acid molecule is as shown in SEQ ID NO: 1-2, SEQ ID NO: 3-4, SEQ ID NO: 5. The corn seed containing the transgenic corn event LP112-1 has been preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: P202432. Maize plants comprising the transgenic maize event LP112-1 of the present invention have the following advantages: free of economic losses due to lepidoptera pests; an agricultural herbicide containing glyphosate can be applied to corn crops; the corn yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof.

The invention belongs to the technical field of pesticide preparation preparation, and discloses a glyphosate isopropylamine salt aqueous solution preparation device and a preparation method.The glyphosate isopropylamine salt aqueous solution preparation device comprises a shell and a support, a stirring device is fixedly arranged on the inner wall of the shell, a cleaning device is arranged on the surface of the stirring device, a filter plate is arranged below the stirring device, and a plurality of feeding devices are evenly arranged above the shell; a circulating device is fixedly arranged on one side above the shell, and a heat exchanger is arranged on the surface of the shell; the problems that in the prior art, mixing is not uniform, precipitated raw materials cannot be subjected to secondary mixing, so that raw materials are wasted, the raw materials cannot be automatically supplemented according to the mixing frequency and the proportion, and the interior of the device is inconvenient to clean are solved, and the device is suitable for preparing the glyphosate isopropylamine salt aqueous solution.

The invention discloses a method for detecting glyphosate residues in coffee in fluorescence and chemiluminescence dual modes, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that succinic acid serves as a ligand, Ce < 4 + > serves as a central ion, a Suc-Ce complex is synthesized through a microwave method, then the Suc-Ce complex is further subjected to alkali treatment to obtain Suc-Ce-OH nano-enzyme, and the Suc-Ce-OH nano-enzyme is used for detecting glyphosate residues in coffee. The Suc-Ce-OH nano-enzyme has excellent phosphatase-like activity, surface coordinated water molecules can be effectively removed through alkali treatment, more Ce-O active sites are exposed, and the hydrolysis efficiency of the Suc-Ce-OH nano-enzyme on a fluorescent substrate 4-MUP and a chemiluminescent substrate AMPPD is improved by two times; based on the specific inhibition effect of glyphosate on phosphatase-like nano-enzyme activity, a glyphosate fluorescence and chemiluminescence dual-mode detection method is established, the detection limits of the glyphosate fluorescence and chemiluminescence dual-mode detection method are 0.05 mu g / mL and 0.01 mu g / mL respectively, and the glyphosate fluorescence and chemiluminescence dual-mode detection method is used for detecting glyphosate residues in a coffee sample and has the advantages of being high in specificity, easy to operate and sensitive.

The invention discloses a method for detecting glyphosate in coffee through Cu-CDs / ABEI (at) Ag nano-enzyme catalytic chemiluminescence, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that 4-aminoantipyrine serves as a precursor, Cu < 2 + > serves as a doping element, copper-doped carbon dots (Cu-CDs) are synthesized through a microwave method, Cu-CDs and ABEI serve as reducing agents to synthesize the Cu-CDs / ABEI (at) Ag nano-enzyme, and the Cu-CDs / ABEI (at) Ag nano-enzyme is used for detecting glyphosate in coffee through Cu-CDs / ABEI (at) Ag nano-enzyme. The Cu-CDs / ABEI (at) Ag nano-enzyme has excellent peroxidase (POD-like) activity, so that H2O2 decomposition is promoted, and a chemiluminescence signal is enhanced; the glyphosate can interact with the Cu-CDs / ABEI (at) Ag nano-enzyme to inhibit the POD-like activity of the nano-enzyme, so that the chemiluminescence intensity generated by oxidation of ABEI is reduced; on the basis, the method is used for detecting glyphosate in a coffee sample, and has the characteristics of high specificity, simplicity in operation and sensitivity.

The present invention relates to a method for post-emergent weed control, which comprises applying an effective amount of a composition comprising herbicide A (component A), glyphosate (component B) and optionally herbicide C (component C) to emerged weeds, or an area, where weeds are growing.

The present invention discloses an EPSPS enzyme mutant, a nucleic acid molecule, an expression cassette, an expression vector, a recombinant bacterium or a recombinant cell and an application thereof, and relates to the technical field of plant genetic engineering. The EPSPS enzyme mutant provided by the present invention can effectively solve the problem of non-selective damage to crops by herbicides such as glyphosate, and provides a new way to cultivate glyphosate-resistant crop varieties. At the same time, the EPSPS enzyme mutant provided by the present invention also has high practicality and promotion value, can improve the weeding effect in agricultural production, reduce the cost of weeding, and promote the increase of crop yield. In addition, the present invention also provides an expression cassette for expressing the EPSPS enzyme mutant, which contains an EPSPS mutant gene that confers glyphosate resistance to various plants such as soybeans and rice.

The present invention relates to the fields of agriculture, plant biotechnology, and molecular biology. Specifically, the present invention relates to a wheat EPSPS mutant protein and use thereof in improving the tolerance of wheat to glyphosate. The mutant protein comprises an amino acid sequence having the following mutations compared to the EPSPS amino acid sequence of any genome of wild-type wheat: in the amino acid sequence set forth in SEQ ID NO: 2, the amino acid at position 168 is mutated from threonine to isoleucine, the amino acid at position 169 is mutated from alanine to valine, and the amino acid at position 172 is mutated from proline to serine. The mutant protein enables the production of stable glyphosate-resistant wheat, showing important significance for wheat breeding.

The invention discloses a method for detecting glyphosate by using a copper monatomic coordination amino covalent organic framework nano-enzyme, the copper monatomic coordination amino covalent organic framework nano-enzyme adopted in the method comprises an amino covalent organic framework, and Cu < 2 + > ions are distributed on a cavity and the surface of the amino covalent organic framework. The nano-enzyme adopted in the invention has the advantages of excellent peroxidase-like activity, high crystallinity, strong chemical stability and the like, can realize specific detection of glyphosate, has the advantages of simple process, convenience in operation, low cost, wide detection range, high detection sensitivity, high detection precision and the like, has the glyphosate detection concentration of 2-100 [mu] mol / L, and has a good application prospect. The detection limit is 0.193 mu mol / L, the method can be widely used for detecting glyphosate in the environment (such as soil or water), the concentration of glyphosate can be rapidly and accurately obtained, the use value is high, and the application prospect is good.

The invention provides an agrobacterium tumefaciens-mediated genetic transformation method for oil palm, which comprises the following steps of: screening and using agrobacterium tumefaciens liquid concentration, dip dyeing time, co-culture time, acetosyringone concentration and antibiotic concentration which are most suitable for genetic transformation of the oil palm in a transformation process in an agrobacterium tumefaciens-mediated manner by taking an oil palm embryonic callus as an explant; an expression cassette containing the EPSPS mutant gene is used as a screening marker, and glyphosate is used as a screening agent for screening, so that the glyphosate-resistant callus is successfully obtained. The screening efficiency of the method exceeds that of a traditional hpt / hygromycin screening system. Meanwhile, the effectiveness of the EPSPS / glyphosate screening system in genetic transformation of the oil palm is also proved, the EPSPS / glyphosate screening system is marked to be applied to genetic transformation of the oil palm for the first time, and a valuable tool is provided for promoting research of an important economic crop.

The present application belongs to the field of pesticides, and particularly relates to a herbicidal composition containing a fused ring substituted aromatic compound and application thereof. The composition comprises a herbicidally effective amount of active ingredient A and active ingredient B, wherein the active ingredient A is or a salt / ester thereof; the active ingredient B is selected from one or more of the following compounds and salts / esters thereof: glyphosate, glufosinate-ammonium, glufosinate-P, paraquat, diquat, ACCase inhibitors, PPO inhibitors, etc. The composition can effectively control / eliminate grass weeds and broadleaf weeds, etc., and has the characteristics of expanding the herbicidal spectrum, reducing the application amount, being able to produce a synergistic effect and solving the problem of resistant weeds, etc.

The invention discloses a weeding composition. The weeding composition comprises glyphosate or a derivative thereof, phosphate and a surfactant component. The phosphate ester is present in an amount of from about 1 to about 15% by weight of the active ingredient based on the total weight of the composition. The surfactant component comprises at least one of a first surfactant, a second surfactant and a third surfactant. Each of the first, second and third surfactants is independently present in an amount of from about 1 to about 15% by weight of the active ingredient based on the total weight of the composition if used.

A herbicidal composition comprises an aqueous solution of a plurality of salts of glyphosate wherein said glyphosate is in anionic form accompanied by low molecular weight non-amphiphilic cations, wherein a major amount but less than 100% of the low molecular weight non-amphiphilic cations are potassium cations, and wherein the composition has a measured pH of at least about 4.8.

The invention relates to the technical field of pesticides, and discloses a glyphosate potassium salt crystal form and a preparation method thereof.The X-ray diffraction pattern of the glyphosate potassium salt crystal form comprises at least one characteristic peak in 2theta of 10.213 + / -0.2 degrees, 20.225 + / -0.2 degrees, 20.867 + / -0.2 degrees, 22.861 + / -0.2 degrees and 23.802 + / -0.2 degrees, the weed withering rate peak value of the glyphosate potassium salt 28 days after pesticide application reaches 98.48%, the weeding performance is excellent, and the glyphosate potassium salt can be applied to the field of pesticides. The crystal form has good stability, the mass change is below 0.5% after the crystal form is stored for 10 days under the conditions of humidity of 65% and room temperature, the crystal form has no obvious hygroscopicity and no obvious change, meanwhile, the crystal form has excellent performance in a long-term stability test, and the crystal form structure does not change after long-term storage.

The invention relates to the technical field of pesticides, and discloses a crystal form of ammonium glyphosate and a preparation method thereof, an X-ray diffraction pattern of the crystal form of ammonium glyphosate comprises at least one characteristic peak in 2theta of 10.196 + / -0.2 degrees, 18.339 + / -0.2 degrees, 20.207 + / -0.2 degrees, 20.857 + / -0.2 degrees, 22.483 + / -0.2 degrees, 22.845 + / -0.2 degrees, 23.416 + / -0.2 degrees, 24.868 + / -0.2 degrees, 26.811 + / -0.2 degrees, 28.878 + / -0.2 degrees or 34.057 + / -0.2 degrees, and the crystal form of ammonium glyphosate is tested to have the characteristic peak of 2theta of 10.196 + / -0.2 degrees, 18.339 + / -0.2 the crystal is regular and high in stability, the control effect reaches 96.79% after the herbicide is applied for 14 days, the control effect reaches 99.05% after the herbicide is applied for 28 days, and the weeding performance is excellent.

The utility model discloses a chloromethane condensate recovery device, and relates to the technical field of chloromethane recovery, the chloromethane condensate recovery device comprises a condensation cavity, the condensation cavity is detachably arranged at the open end of a reaction kettle, a condensation assembly is arranged in the condensation cavity, and the condensation assembly comprises a conical hopper, a rotating shaft and a cooling ring, a heat insulation ring is arranged at the edge of the bottom of the condensation cavity, a conical hopper is arranged at the top of the heat insulation ring, a rotating cylinder is rotationally connected to the top of the condensation cavity, the rotating shaft penetrates through the rotating cylinder, and a plurality of fan blades are arranged on the side face of the rotating shaft in the circumferential direction and located at the upper end of the interior of the conical hopper. According to the utility model, the condensation cavity for condensing waste gas and the reaction kettle for preparing glyphosate are integrated into one cavity, so that the occupied space is reduced, the waste gas automatically moves towards the condensation cavity in the reaction kettle, and the moving speed of the waste gas between the thermal insulation ring and the condensation cavity is increased through the rotation of the fan blades, so that the condensation efficiency of the waste gas is improved.

The present invention discloses a corn EPSPS enzyme mutant, a nucleic acid molecule, an expression cassette, an expression vector, a recombinant bacterium and its application, and relates to the field of plant genetic engineering technology. The present invention obtains a glyphosate-resistant corn EPSPS enzyme by mutating the wild-type corn EPSPS enzyme to Q62H, R122G, V155M, G163A, P168S, V222A, P229S and V394A. After the wild-type corn EPSPS enzyme is transformed into a plant, the corn EPSPS enzyme mutant can be expressed and has glyphosate resistance. The present invention proposes an effective solution to the problem of non-selective damage to crops by herbicides such as glyphosate, and provides a new approach for cultivating glyphosate-resistant crop varieties. At the same time, the corn EPSPS enzyme mutant provided by the present invention also has high practicality and promotion value, can improve the weeding effect in agricultural production, reduce the cost of weeding, and promote the increase of crop yield.

The invention relates to the field of herbicides, and provides soluble powder for weeding, which is prepared from the following components in parts by weight: 170 to 220 parts of triclopyr raw medicine, 40 to 70 parts of potassium hydroxide, 450 to 550 parts of glyphosate raw medicine, 250 to 350 parts of ammonium bicarbonate, 100 to 150 parts of ammonium sulfate and 60 to 120 parts of auxiliaries. The invention also provides a preparation method of the composition. The triclopyr and the glyphosate have different action mechanisms and weeding spectrums, so that the weeding range can be expanded, the weeding effect can be enhanced and the generation of drug resistance can be slowed down through the cooperative use of the triclopyr and the glyphosate; due to the conductivity of the triclopyr, the triclopyr can go deep into weed bodies, the glyphosate can be widely distributed in the weed bodies, and the triclopyr and the glyphosate are combined for use, so that respective advantages can be better exerted; the weeding soluble powder obtained by compounding the raw materials has the advantages of high efficiency, broad spectrum, rain wash resistance, freezing resistance, stability, good processability during granulation and the like, and still has the characteristic of high solubility at low temperature.

The invention relates to the technical field of glyphosate preparation, in particular to a glyphosate preparation process by a glycine method and application of the glyphosate preparation process, and the glyphosate preparation process at least comprises the following steps: carrying out dealcoholization, deacidification, crystallization, centrifugation and drying on glyphosate acidified liquid to obtain glyphosate; the crystallization step comprises the following steps: S1, transferring deacidification liquid obtained after the deacidification step to a crystallization kettle, stirring materials in the crystallization kettle, and cooling to 60-70 DEG C; s2, keeping the temperature of the material constant at 60-70 DEG C, and dropwise adding liquid caustic soda into the crystallization kettle; and S3, after the dropwise addition of the liquid caustic soda is completed, continuously stirring and cooling to 25-30 DEG C, and carrying out heat preservation to complete the crystallization step. The novel process for preparing glyphosate by using the glycine method can solve the problem that the yield and quality of a glyphosate product are affected due to incomplete deacidification in a deacidification process in a conventional process, the content of the obtained glyphosate is greater than 96%, and the yield is greater than 76%.

The application discloses a preparation method of carbon dot nanometer enzyme, which comprises the following steps: dissolving oxalic acid, polyacetyl imine and ferric nitrate in ultrapure water, heating and mixing, cooling to room temperature, centrifuging, filtering, dialyzing and taking supernatant to obtain the carbon dot nanometer enzyme; and a double-mode detection method for colorimetric and fluorescent detection of glyphosate in fruits and vegetables is constructed by using the prepared oxalic acid carbon dot nanometer enzyme, acetylcholinesterase, acetylcholine, 3,3',5,5'-tetramethyl benzidine and H2O2. The oxalic acid carbon dot nanometer enzyme is prepared by doping ferric nitrate and tetraethylenepentamine with oxalic acid as a precursor, has excellent peroxidase-like activity, and a colorimetric and fluorescent double-mode detection method for glyphosate is constructed by using the oxalic acid carbon dot nanometer enzyme, the double-mode detection results can be verified with each other, and the accuracy and reliability of the detection results are significantly improved.

Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic / transgene junctions within SEQ ID NO: 2 or with genomic / transgene junctions as set forth in SEQ ID NO: 12 and / or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

The present invention relates to the fields of chemistry, agriculture, and environmental protection, and discloses a fluorescent probe and its use in fluorescent detection of glyphosate, specifically a fluorescent probe technology for rapid detection of glyphosate in water and agricultural products. The colorimetric fluorescent probe, which combines a 2-chloroaniline sensing unit with the fluorophore dansyl chloride, induces fluorescence enhancement through a substitution reaction with glyphosate. Specifically, the probe can detect glyphosate in the range of 0 to 70 μmol·L. ‑1 Fluorescence titration experiments were conducted with glyphosate at varying concentrations within a specific range. With the gradual addition of glyphosate, the probe's fluorescence spectrum rapidly increased, establishing a satisfactory relationship between fluorescence intensity and glyphosate concentration. Common anions and metal ions found in water did not interfere with the reaction, demonstrating the detection method's excellent anti-interference properties. Furthermore, this probe was used to detect glyphosate in tap water, Xiangxi River Reservoir, soil, soybeans, and corn, with recoveries ranging from 94.7% to 109.9%. This open-type fluorescent probe can specifically detect glyphosate with the naked eye under ultraviolet light.

This invention discloses a glyphosate resistance-related protein, GAT_656, and its related biomaterials and applications. The protein GAT_656 is one of the following: a) a) a protein with the amino acid sequence shown in Sequence 1; b) a fusion protein obtained by attaching a tag to the N-terminus and / or C-terminus of the protein shown in Sequence 1; c) a protein with the same function obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown in Sequence 1; d) a protein with 75% or more identity to the amino acid sequence shown in Sequence 1 and with the same function. The protein GAT_656 and its related biomaterials of this invention can be used to breed novel glyphosate-resistant crop varieties, providing an efficient and stable solution for glyphosate resistance in crops, contributing to the improvement of agricultural production efficiency and sustainable development, and possessing significant application value.

The invention relates to the technical field of rice breeding, in particular to application of rice EPSPS protein point mutation in improving glyphosate resistance of rice. Point mutation is located at the 410th site and the 414th site of the rice EPSPS protein, the amino acid at the 410th site mutates from V to D, and the amino acid at the 414th site mutates from A to D and the like. V410D / A414D and P227Q / L509V mutation modes generated by natural variation of the rice EPSPS gene are reported for the first time, the physical and chemical properties and the protein structure of the rice EPSPS gene are found to be changed, and transgenosis verifies that the two mutations can remarkably improve the glyphosate resistance of the rice, and a new target is provided for molecular design of glyphosate-resistant rice.

The invention belongs to the field of preventing tillers of old citrus tree stump heads, and particularly relates to a method for preventing tillers of old citrus tree stump heads, which comprises the following steps: step 1, sawing the stump heads by using a saw or chopping the stump heads by using an axe, so that the surfaces are as flat as possible, the whole stump heads are kept 10cm away from the ground, and fresh sections are exposed; step 2, brushing glyphosate (not diluted) at the saw bite of the tree stump by using a brush; thirdly, a 15S black plastic film is adopted for pile head covering (0.7 m * 0.7 m); 4, covering soil with a film, and treading tightly. The method solves the problems that the prevention and treatment effect is reduced due to different reasons in production, for example, lime powder and herbicide can only effectively kill tissues at wounds of stumps, and the stumps can germinate new tillers after one year; and the fire burning effect is unstable, and the health of fruit farmers is easily harmed due to improper operation.