21 results about "Genetically modified crops" patented technology

The invention belongs to the technical field of biological detection, and discloses a transgenic crop intelligent identification method based on high-throughput sequencing, which comprises the following steps: acquiring multi-platform high-throughput sequencing original data and sample metadata, evaluating quality by platforms, dividing according to a sample partitioning rule, and generating a quality-controlled data block set; distributing data blocks to a plurality of parallel computing nodes, synchronously executing technical detection to obtain technical fact fragments, aggregating and supplementing batch statistical information, and generating a technical fact report; matching the judgment rule set, carrying out parallel judgment, triggering expert rechecking on boundary cases, and integrating to generate a supervision judgment conclusion set; the method comprises the following steps: extracting original sequencing reads of all samples, generating a unique hash for each read, constructing a processing track chain, integrating metadata and visual evidence, generating a credible authentication report, dividing different scene versions, and performing targeted distribution; and extracting identification cases for mining and clustering, generating optimization suggestions, and feeding back the optimization suggestions to the quality control rule base and the judgment rule base.

The application belongs to the technical field of molecular biology, and particularly relates to a nucleic acid rapid extraction kit for transgenic detection of high-oil crops and application, and is particularly suitable for fluorescent quantitative PCR detection of transgenic components of high-oil crops. The kit comprises a lysis solution, a precipitation solution, a neutralization solution and an elution solution. The application can be completed within 15 minutes, effectively removes PCR inhibitors such as oil and protein, is specifically used for high-oil crops, and is a low-cost, safe and non-toxic nucleic acid rapid extraction method, and meets the requirement of on-site rapid detection of transgenic crops.

The application belongs to the field of agricultural biotechnology, and discloses a database construction and analysis method for soybean transgenic crops, which comprises the following steps: obtaining soybean high-throughput sequencing original data and metadata, performing soybean specificity quality control, storing in a database sub-database according to a scene label, and generating standardized sub-database data; identifying the priority of each sub-database scene, allocating hardware according to a priority-resource matching rule, generating resource configuration records, synchronously executing multi-dimensional feature extraction, and generating a multi-dimensional feature dataset; dynamically adjusting the feature importance degree according to the scene, performing feature collaborative analysis, combining an enhanced decision knowledge base to retrieve and match results and solving feature conflicts, and generating a dynamic confidence identification report; extracting key feature data and identification process information to generate a hash value, writing the hash value into a consortium chain, and associating a soybean sample ID to form a digital traceability certificate; dynamically optimizing the feature importance degree, the enhanced decision knowledge base and the database sub-database rule, and forming a full-link iteration.

The invention provides a crop allergen dynamic baseline construction and trusted sharing system based on a multi-dimensional heterogeneous data cube, and the system comprises a collection layer which is used for collecting physical information metadata of a to-be-detected sample based on an agricultural environment monitoring terminal, the method comprises the following steps: performing physical detection on a to-be-detected sample through a high-throughput sequencing terminal to generate original mass spectrum data and transcriptome data, and calling meteorological metadata of a region where the to-be-detected sample is located through a third-party meteorological server; the core processing layer is used for generating a dynamic baseline by adopting a central processing server cluster based on the physical information metadata, the original mass spectrum data, the transcriptome data and the meteorological metadata; and the application layer is used for checking the dynamic baseline based on the user query terminal and checking the GLP log based on the checking and auditing terminal. By constructing the multi-dimensional heterogeneous data cube and the dynamic baseline model, standardized data calculation service is provided for safety evaluation of transgenic crops and biotechnology products.

This invention relates to the field of plant transgenic detection technology, and discloses a method for rapid batch detection of transgenic crops, comprising: Step 1, placing centrifuge tubes containing crop samples to be tested in an array into a multi-well plate to complete batch plate arrangement of samples; Step 2, performing overall mechanized processing based on the multi-well plate, so that the crop samples in the centrifuge tubes are simultaneously ground into powder under mechanical force. This integrated operation process, which places independent centrifuge tubes in an array within a multi-well plate and uses a tissue homogenizer, batch liquid addition equipment, and a centrifuge to simultaneously process all samples, achieves a significant improvement in detection throughput and efficiency. Compared to existing technologies that rely on manual, one-by-one operation, this method overcomes the shortcomings of low detection efficiency and the inability to simultaneously process multiple samples, thus failing to meet the needs of large-scale crop sample screening.

Provided in the present invention is a hypermorphic allele ETD1 against rice blast, wherein the ETD1 gene has a nucleotide sequence as set forth in SEQ ID NO: 1, and a cDNA sequence thereof is as set forth in SEQ ID NO: 2. Further provided is an encoded protein of the gene, wherein the protein has an amino acid sequence as set forth in SEQ ID NO: 3. The gene is a hypermorphic allele of OsCNGC13, which exhibits enhanced calcium-ion transport capacity and has the function of significantly accelerating the influx of calcium ions into cells. Therefore, the normal expression or overexpression of the protein in a gene knockout plant can enhance resistance of rice, and thus the protein has a positive regulatory effect on rice blast resistance. The present invention can be used for the rapid identification of transgenic crops harboring ETD1 mutant genes, thereby improving the efficiency of molecular biological breeding.

Various examples relate to a transgenic crop plant where a sequence of a cis-regulatory element in the promoter of certain genes is genomically changed in order to alter the flowering time or flowering rate of the plant. In one example, the cis-regulatory element in the promoter of such certain genes may be modified from TGAATG(A / T) (A / T / C) to TGAGGG(A / T) (A / T / C). The certain genes may be genes that encode SbEhd1, OsFTL10 / FT8, SbFT10, ZmELF3.2, or combinations thereof. And, the crop plant can be for example, sorghum, maize or rice. In another example, the present transgenic crop plant is a progeny plant. In yet another example, flowering time of the transgenic crop plant is regulated by a method that includes changing the sequence of the cis-regulatory element in the promoter of certain genes.

The present invention relates to a method for the relative quantification of GM crops using digital PCR without using certified reference materials (CRMs). More specifically, the present invention relates to a primer and probe set composition for digital PCR for the relative quantification of transgenes in GM crops without using CRMs, which comprises a first primer and probe set capable of specifically detecting genes present in a single copy or in 2-3 copies within the genome of GM (genetically modified) crops; and a second primer and probe set capable of specifically detecting transgenes in GM crops, and uses thereof.

The invention discloses a method for controllably removing an exogenous vector skeleton and a selection marker. According to the method, a novel transformation vector sequence containing an isolated expression cassette sequence composed of a non-autonomous transposon and a target gene is designed, and the isolated expression cassette is inserted into a corn kernel purple aleurone layer marker gene sequence to form a target gene expression cassette transposition detection system. Meanwhile, a fluorescent protein coding gene specifically expressed by a grain aleurone layer and a selection marker gene sequence are utilized to form a vector skeleton detection system. According to the method, a plurality of new ultra-pure transformant events can be obtained from one initial transformation event, the safety evaluation cost and time are saved, the number of transformation events is increased, and the method has great value for accelerating and promoting industrialization of transgenic crops.

The invention belongs to the technical field of plant biology, and particularly relates to an amaranthus retroflexus ArCYP81Q32 gene and application thereof. The invention discovers that amaranthus retroflexus ArCYP81Q32 participates in the degradation of a metabolic detoxification protoporphyrinogen oxidase (PPO) inhibitor herbicide fluoroglycofen-ethyl in a plant body. The relationship between the amaranthus retroflexus ArCYP81Q32 gene and fluoroglycofen-ethyl resistance is disclosed for the first time, whether a plant material generates metabolic resistance to herbicides or not can be identified by detecting the expression quantity of the amaranthus retroflexus ArCYP81Q32 gene, and the method has guiding significance on timely discovery of resistance, guidance of scientific pesticide application in farmland and alleviation of further spreading of pesticide resistance. The amaranthus retroflexus ArCYP81Q32 gene detoxifies protoporphyrinogen oxidase inhibitor herbicides through metabolism, and also provides a new gene resource for developing transgenic crops resisting the herbicides.

The present application discloses a polynucleotide, a method and a kit for detecting transgenic crops. Specifically, the present application discloses a polynucleotide construct pLW10 and its matching primer probe sequence which can be used as a standard molecule for real-time fluorescent PCR detection of transgenic crops. The plasmid standard molecule of the present application solves the problem of lack of standard substance in real-time fluorescent PCR detection of transgenic crops, ensures the comparability of the detection results of real-time fluorescent PCR method of transgenic crops, and provides a reliable quality control method for real-time fluorescent PCR method detection of transgenic crops.

The present application belongs to the technical field of biological detection, and discloses a transgenic crop intelligent identification method based on high-throughput sequencing, which comprises the following steps: obtaining multi-platform high-throughput sequencing original data and sample metadata, evaluating quality in a platform-by-platform manner, dividing according to sample block rules, and generating a quality-controlled data block set; distributing the data block to multiple parallel computing nodes, synchronously executing technical detection to obtain technical fact segments, aggregating and supplementing batch statistical information, and generating a technical fact report; matching a judgment rule set, performing parallel judgment, triggering expert review for boundary cases, and then integrating to generate a supervision judgment conclusion set; extracting all sample original sequencing reads, generating a unique hash for each read, constructing a processing track chain, integrating metadata and visualized evidence, generating a credible identification report and dividing different scenario versions, and performing targeted distribution; extracting identification cases for mining and clustering, and generating optimization suggestions to feed back to a quality control rule library and a judgment rule library.

The present application relates to the field of biotechnology, in particular to a method for heterologous synthesis of dammarane-type ginsenosides in the endosperm of crop seeds, a vector, a plant and application. Through modular design, a core gene module driven by an endosperm-specific promoter is assembled in the T-DNA region of the vector, and a downstream glycosylation or hydroxylation-glycosylation modification module is configured to realize the directional synthesis of PPD-type and PPT-type ginsenosides, respectively. The transgenic crops obtained by using the vector can specifically accumulate target ginsenosides in the endosperm. The obtained ginseng rice shows clear anti-tumor activity in in vitro tumor cell experiments and tumor-bearing mouse models. The present application provides a new strategy for low-cost production of high-value natural products, and opens up a new direction for the application of functional crops in the field of medicine.

The invention discloses a cyhalofop-butyl-resistant digitaria calorata MYB transcription factor gene and application thereof, and relates to the field of plant genetic engineering, and the expression of the gene in a resistant digitaria calorata biological type is remarkably up-regulated. After a plant expression vector is constructed and corn is transformed, after 11.25 g a.i. Ha-1 cyhalofop-butyl is sprayed on a transgenic corn plant in a 2-leaf stage, the pesticide resistance and the survival rate are obviously enhanced, and the transgenic corn plant is obviously superior to a wild type. The invention proves that the MYB gene can endow plants with resistance to acetyl-coenzyme A carboxylase (ACCase) inhibitor herbicides such as cyhalofop-butyl and the like, provides a new gene resource for cultivating transgenic crops resisting the acetyl-coenzyme A carboxylase (ACCase) inhibitor herbicides, and has important application value.

The invention discloses an active polypeptide tolerant to PPO inhibitor herbicides and application, the active polypeptide has at least 85%, 90%, 95%, 99% and 100% sequence identity of an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2, and has protoporphyrinogen oxidase activity. The active polypeptide shows high tolerance to PPO inhibitor herbicides, and obtained transgenic crops simultaneously resist various herbicides such as a PPO inhibitor, an EPSPS inhibitor, an ACC enzyme inhibitor, an ALS inhibitor and synthetic auxin, and have broad-spectrum herbicide resistance; in the weed control process, the PPO inhibitor herbicide is used in combination with one or more other herbicides, so that the cost can be effectively reduced, the control efficiency can be improved, and the weed resistance can be greatly avoided.

The present disclosure relates to the use of substituted uracil pyridines or agrochemically acceptable salts thereof, for controlling or preventing weed growth in plant growth areas of transgenic crop plants that are tolerant to PPO inhibiting herbicides. The disclosure also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

The present disclosure relates to the use of substituted uracil pyridines or agrochemically acceptable salts thereof, for controlling or preventing weed growth in plant growth areas of transgenic crop plants that are tolerant to PPO inhibiting herbicides. The disclosure also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

The invention discloses a primer composition, a micro-fluidic chip, a kit and a method for simultaneously detecting multiple food transgenic elements. The primer composition comprises the following primer groups: a primer group for detecting a transgenic element pCaMV35S, a primer group for detecting a transgenic element pUBI, a primer group for detecting a transgenic element pNOS, a primer group for detecting a transgenic element pRBCS4, a primer group for detecting a transgenic element pFMV35S and a primer group for detecting a transgenic element tNOS. The invention discloses a primer group for detecting a transgenic element tpin II. The detection device can simultaneously detect a plurality of target transgenic elements of a plurality of samples, can cover a plurality of transgenic crops, has the advantages of short detection time, high sensitivity and the like, is small in size, light in weight and convenient to carry, and can be applied to rapid field detection.

A siRNA targeting a key gene of Magnaporthe oryzae for controlling rice blast and use thereof, and relates to the field of biotechnology. The siRNA targets silence of a MOHS1 gene (MGG_08523) of Magnaporthe oryzae. The siRNA molecule can delay and inhibit the formation of an appressorium of the Magnaporthe oryzae, thereby reducing the pathogenicity of the Magnaporthe oryzae, thereby providing a novel method for controlling the rice blast. The siRNA molecule targeting the MOHS1 gene is prepared into a nucleic acid pesticide, and the nucleic acid pesticide is sprayed onto leaves of Oryza sativa L., which effectively reduces the number and area of lesions of the rice blast on the leaves, in turn can be used for controlling the rice blast, and can avoid a tedious process of cultivating genetically modified crops and the concerns of consumer groups.

The invention discloses a method for detecting a transgenic element CaMV 35S promoter and an NOS terminator based on an LAMP-OSD probe method, and relates to the technical field of molecular biology. The LAMP-OSD probe method is used for rapidly and sensitively detecting whether a plant contains a transgenic element CaMV 35S promoter and / or NOS terminator or not, and the specific method disclosed by the invention comprises the following steps: extracting DNA of a sample to be detected as a template, and carrying out single rapid amplification probe specific recognition / double rapid amplification probe specific recognition by utilizing a primer pair and a probe combination, and carrying out real-time fluorescence detection, if fluorescence is observed, determining that the plant is positive, if a CaMV 35S promoter and / or NOS terminator exists, determining that the plant belongs to a transgenic crop, and otherwise, determining that the plant is negative.

The utility model belongs to the technical field of insect catching and killing, and discloses an arthropod suction device for transgenic crop fields, which comprises a connector (1), a suction tube (2), bristles (3) and suction holes (4). The connector (1) is fixed at one end of the suction tube (2), a suction hole (4) is formed in the surface of the connector (1), and a plurality of clusters of bristles (3) are arranged around the suction hole (4). The arthropod suction device can be used for efficiently sucking arthropods in fields of crops such as cotton and soybeans, and has the advantages of being simple in structure, low in cost, capable of improving the arthropod suction efficiency and the like.