341 results about "Genetically modified crops" patented technology

The invention relates to a method for the control of weeds at a crop locus, said method comprising the application of an effective amount of: (a) a glyphosate herbicide which is glyphosate or a derivative thereof; and (b) at least one HPPD-inhibiting herbicide; wherein the crop is tolerant to glyphosate and optionally the HPPD-inhibiting herbicide.

A genetically modified non-human animal is provided, wherein the non-human animal expresses an antibody repertoire capable of pH dependent binding to antigens upon immunization. A genetically modified non-human animal is provided that expresses human immunoglobulin light chain variable domains derived from a limited repertoire of human immunoglobulin light chain variable gene segments that comprise histidine modifications in their germline sequence. Methods of making non-human animals that express antibodies comprising histidine residues encoded by histidine codons introduced into immunoglobulin light chain nucleotide sequences are provided.

A genetically modified non-human animal is provided, wherein the non-human animal expresses an antibody repertoire capable of pH dependent binding to antigens upon immunization. A genetically modified non-human animal is provided that expresses a single light chain variable domain derived from a single rearranged light chain variable region gene in the germline of the non-human animal, wherein the single rearranged light chain variable region gene comprises a substitution of at least one non-histidine encoding codon with a histidine encoding codon. Methods of making non-human animals that express antibodies comprising a histidine-containing universal light chain are provided.

The invention discloses a method for determining influence of exogenous dsRNA on toxicity of ladybugs. The method comprises the following specific steps: uniformly mixing the exogenous dsRNA into a sucrose solution, directly feeding the ladybugs for 2-3 days and then feeding with pea aphids; then detecting and analyzing the expression changes of target genes in the ladybugs, and observing the biological changes of the ladybugs so as to evaluate the toxicity of the exogenous dsRNA to the ladybugs, wherein the ladybugs include harmonia axyridis, coccinella septempunctata or henosepilachna pusillanima. By the method, direct influence of the exogenous dsRNA on the toxicity of the harmonia axyridis, the coccinella septempunctata or the henosepilachna pusillanima or other ladybugs can be effectively determined; in addition, the method is simple, feasible and high in effectiveness and sensitivity, and has a great significance and an application prospect in study on functions of related genesas well as environmental risk assessment on related RNAi transgenic crops.

The invention discloses a Pteromalus puparum venom serine protease inhibitor Pp-PI polypeptide which has an amino acid sequence shown in SEQ ID NO: 2. At the same time, the invention also discloses a gene for encoding the Pteromalus puparum venom serine protease inhibitor Pp-PI polypeptide. The gene has a nucleotide sequence from the 126th nucleotide to the 290th nucleotide in SEQ ID NO: 1; or the gene has at least 70% homology with the nucleotide sequence from the 126th nucleotide to the 290th nucleotide in SEQ ID NO: 1; or the gene has a nucleotide sequence which can hybridize with the nucleotide sequence from the 126th nucleotide to the 290th nucleotide in SEQ ID NO: 1 at 40-55 DEG C. The Pteromalus puparum venom serine protease inhibitor Pp-PI polypeptide can be used to prepare a Pteromalus puparum venom serine protease inhibitor for genetically modifying crops or altering plant symbiotic bacteria.

The invention provides an acquisition method for transgenic gramineous crops capable of being selectively perished, comprising the following steps: when target genes are expressed, expression of genes participating in herbicide detoxification in the transgenic gramineous crops is inhibited by utilization of antisense RNA or RNAi means, and the transgenic gramineous crops capable of being selectively perished are obtained. The invention mainly has the advantages that: the transgenic gramineous crops acquired by utilization of the method can be selectively perished by Bentazon or sulphuryl carbamide herbicide as required, thereby transmission of the transgenic gramineous crops and interfusion to non-transgenic gramineous crops are prevented, and the method has great significance in transgenic crop research later on.

The present invention discloses Resistance Management (RM) practices that are critical to safeguarding Bacillus thuringiensis as a natural resource and to sustaining genetically modified crops that express Bt toxins for managing ECB and WCRW. The methods involve blending seed transformed with a nucleic acid encoding a different pesticidal protein, where both proteins target the same pest, but use different modes of pesticidal action. The seed can be also treated with pesticidal agents.

Methods and compositions for deploying refuge seeds together with transgenic crop seeds are provided. The refuge seeds can be non-transgenic seeds of a similar variety to that of the transgenic crop seeds, or the refuge seeds can be a transgenic variety.

The invention discloses a positive plasmid molecule pBI121-Screening and application thereof and relates to the technical field of safety supervision and screening detection of transgenic crops in the transgenic safety field. The base sequence of the pBI121-Screening is as shown in SEQ NO.1. serving as both a positive contrast and a quality control sample, pBI121-Screening disclosed by the invention can be respectively used in general PCR and real-time fluorescence PCR screening detection of transgenic components of six transgenic crop samples, namely paddy rice, rapes, soybeans, corns, cotton and wheat. The pBI121-Screening disclosed by the invention can be used for solving the technical problem that the lack of a positive contrast or a standard sample in the screening detection of the transgenic crops, and prevents the problem that a plurality of positive contrasts are arranged for a plurality of detection targets in the screening detection, thus reducing the labor cost and economic cost for preparing the plurality of positive contrasts.

The invention provides a pair of hybridoma cell strains. The hybridoma cell strains comprise the first hybridoma cell strain and the second hybridoma cell strain which are stored independently. The preservation number of the first hybridoma cell strain is CGMCC NO.10495. The preservation number of the second hybridoma cell strain is CGMCC NO.10494. The invention further provides a pair of paired monoclonal antibodies excreted by the two hybridoma cell strains, an application of the paired monoclonal antibodies in detecting G2-EPSPS protein and immuno gold staining test paper. According to the technical scheme, the sensitivity of 1 ng/mL can be obtained through detection of the immuno gold staining test paper on G2-EPSPS protein, no cross reaction is carried out on CP4-EPSPS protein, no cross reaction is carried out on 27 non-GMO crops and 14 G2-EPSPS transgenic crops with different sources, and high sensitivity and specificity are achieved.

The invention relates to cotton GbVe gene and a protein coded thereby. In the invention, a Ve gene associated with verticillium wilt resistance is obtained by cloning in a gossypium barbadense variety having high verticillium wilt resistance; a 5'-terminal GbVe gene fragment and a 3'-terminal GbVe gene fragment, which are obtained by amplification, are spliced; a primer capable of amplifying the full-length GbVe gene is designed and synthesized according to a gene sequence; the sequence of the full-length GbVe gene is obtained by the conventional colony sequencing method according to an RT-PCR technique; and the nucleotide sequence of the full-length GbVe gene is represented by SEQ ID No.1. In the invention; a new and important candidate gen is provided for vegetable verticillium wilt resistant gene project; and the obtained full-length GbVe gene and a vegetable expression vector constructed by the full-length GbVe gene lay a foundation for further transforming important crops for improving the verticillium wilt resistance of transgenic crops and have great economic benefits and application values.

The invention provides a herbicide-containing fertilizer and a preparation method and use thereof. The herbicide-containing fertilizer, based on 100 percent of total mass, consists of the following formula components in percentage by weight: 15 to 40 percent of glyphosate, 0.4 to 8 percent of trace element compound, 5 to 15 percent of surfactant, 0.5 to 20 percent of stabilizer and the balance of filler. In the herbicide-containing fertilizer provided by the embodiment of the invention, because the glyphosate, trace element component and the like are mixed reasonably, the soil fertility, resistance in crops (particularly glyphosate resistant transgenic corps) and crop yield are all increased, and the crop yield is improved by up to 30 percent; and the herbicide effectively lowers the labor intensity and manual cost of agricultural production. In addition, the herbicide-containing fertilizer is produced by mixing by group or mixing by crushing, so the dispersion uniformity of the components is improved effectively, the preparation method is simple in process and low in energy consumption and the production cost is lowered; and the method is suitable for industrial production.

The invention discloses a protein chip for detecting transgenic products. The protein chip is a film which is arranged on the plane of a plane type supporting carrier and coated with a modified material; and protein antibody probes for detecting transgenic agricultural products are arranged on the surface of the film in a lattice mode and comprise 1) a Bt cry1Ac/Ab monoclonal antibody, 2) a Bt cry1Ah monoclonal antibody, 3) a phytase PHY monoclonal antibody, 4) a glyphosate-tolerant G2-EPSPS polyclonal antibody and 5) a salt-tolerant IrrE polyclonal antibody. The invention further provides a chip kit for detecting transgenic crops and performing result judgment simply and rapidly based on antigen antibody reaction, which belongs to the field of inspection and quarantine. The protein chip kit has the advantages of: 1) high speed, wherein the detection time is only 1.5 to 2 hours; 2) specificity, wherein protein antigens and antibodies are specifically combined and each antibody performs a point system process repeatedly for three times, so that the result correctness is greatly improved; 3) high flux, wherein the protein chip kit can detect the proteins of five kinds of commercial transgenic components at the same time; and 4) stability, wherein the results of the repeated tests show that the stability of the method is high.

The invention provides a method for detecting the number of arbuscular mycorrhizal fungi in wheat rhizosphere soil based on real-time fluorescence quantification PCR. The method includes the steps that firstly, sample total DNA of wheat rhizosphere soil is extracted and serves as a template of PCR amplification; then, amplification is conducted with SEQ ID No.1 and SEQ ID No. 2 as primers, and specific fragments of 129 bp are obtained; after bond and conversion are conducted, qPCR amplification is conducted. By means of the method, arbuscular mycorrhiza is authenticated, absolute quantification can be conducted on the arbuscular mycorrhiza accurately, the copy number of the arbuscular mycorrhizal fungi in all periods of duration of transgenic wheat is obtained, and in the growth and development stages of wheat, the arbuscular mycorrhiza copy number tends to be gradually increased as a whole. The rapid, convenient and precise authentication method is built, a theoretical and test basis is provided for subsequent tests, great significance is provided for further evaluation of safety of transgenic crops, and compared with an existing method, the method is high in sensitivity, high in specificity, good in repeatability, convenient to operate and visual in result.

The invention discloses a Biolog ECO micro-plate technique applied to soil microecological safety evaluation for genetically modified (GM) crops. The Biolog ECO micro-plate technique comprises the following steps: (1) collecting rhizosphere soil samples of the GM crops and non-GM parent crops during different childbearing periods; (2) performing Biolog ECO experiments; (3) calculating average well color development (AWCD) and diversity indexes; and (4) performing principal component analysis (PCA). The Biolog ECO micro-plate technique can be used for evaluating the influence of the GM crops on functions of rhizosphere soil microbial communities so as to evaluate the potential security risk of the GM crops for a rhizosphere soil microecosystem.

The present disclosure relates to methods for non-destructively assessing yield and/or stress tolerance in crop plants through the use of high-energy particle based imaging and analysis including X rays. Also provided are methods to non-destructively assess transgenic crop plants for the effects of a transgene on yield and/or on stress tolerance.

The invention discloses a liquid phase chip for detecting Bt cry1 Ac protein and its application. The liquid phase chip provided by the present invention includes fluorescent microspheres coated with anti-Bt cry1 Ac monoclonal antibodies, biotinylated monoclonal antibodies against different antigenic epitopes of Bt cry1 Ac, and streptavidin phycoerythrin. The present invention also provides using the liquid phase chip to detect whether the plant is a transgenic Bt cry1 Ac plant. The present invention utilizes a monoclonal antibody against Bt cry1 Ac and adopts a double antibody sandwich method to establish a liquid phase chip detection method for Btcry1 Ac protein in transgenic crops (GMO). Good repeatability.

The invention relates to an insecticidal protein Cry1m7, which is 1) a protein composed of an amino acid sequence shown by SEQ ID NO:1, or 2) a protein which is obtained by substituting, deleting or inserting one or more amino acid sequences in the amino acid sequence shown in SEQ ID NO:1 and has an equivalent activity. The invention further provides a coded gene, an expression vector and an engineering bacterium of the insecticidal protein, an application of the protein to insecticides and an application of the coded gene of the protein to plant conversion. The invention provides the high-toxicity insecticidal protein Cry1m7 and the coded gene thereof; and the insecticidal protein provided by the invention can be used for killing Asiatic corn borers and other lepidoptera pests to improve the insecticidal capability of genetically modified crops.

The invention provides a method for detecting wheat yellow mosaic virus-resisting transgenic wheat metabolites on the basis of gas chromatography-mass spectrometry technology (GC-MS). The wheat yellow mosaic virus-resisting transgenic wheat metabolites are optimized on the basis of the GC-MS detection method, the type and proportion of an extracted solution, extracting time, volume of the extracted solution, deriving process temperature and time are respectively optimized, the relative peak area is at an optimal detection state under the extracting condition of the extracted solution that the ratio of water to acetonitrile to isopropanol is 2:3:3 when 1mL of extracted solution is added for extracting for 20min, and more metabolites components can be detected. Under the deriving condition that the temperature is 30DEG C and the extracting time is 90min, the relative peak area is large, and the signal intensity is obvious. By adopting the detection method, reliable and effective data can be provided for the study of the metabonomics of the wheat yellow mosaic virus-resisting transgene wheat, and a foundation can be laid for the safety evaluation of the transgenic crop.