42 results about "Genetically modified crops" patented technology

The invention belongs to the technical field of biological detection, and discloses a transgenic crop intelligent identification method based on high-throughput sequencing, which comprises the following steps: acquiring multi-platform high-throughput sequencing original data and sample metadata, evaluating quality by platforms, dividing according to a sample partitioning rule, and generating a quality-controlled data block set; distributing data blocks to a plurality of parallel computing nodes, synchronously executing technical detection to obtain technical fact fragments, aggregating and supplementing batch statistical information, and generating a technical fact report; matching the judgment rule set, carrying out parallel judgment, triggering expert rechecking on boundary cases, and integrating to generate a supervision judgment conclusion set; the method comprises the following steps: extracting original sequencing reads of all samples, generating a unique hash for each read, constructing a processing track chain, integrating metadata and visual evidence, generating a credible authentication report, dividing different scene versions, and performing targeted distribution; and extracting identification cases for mining and clustering, generating optimization suggestions, and feeding back the optimization suggestions to the quality control rule base and the judgment rule base.

This invention provides an siRNA nucleic acid pesticide targeting the DICER2 gene to control rice blast fungus, belonging to the field of biotechnology. The siRNA molecule can delay and inhibit the formation of rice blast fungus appressoria, thereby reducing the pathogenicity of the fungus and providing a new method for controlling rice blast. When used in combination with tricyclazole or clotrimazole, it has a synergistic effect. The siRNA molecule targeting the DICER2 gene is prepared into a nucleic acid pesticide and sprayed on rice leaves, effectively reducing rice blast lesions and their area, thus enabling its use in rice blast control. Furthermore, it avoids the cumbersome process of cultivating genetically modified crops and addresses consumer concerns.

The application belongs to the technical field of molecular biology, and particularly relates to a nucleic acid rapid extraction kit for transgenic detection of high-oil crops and application, and is particularly suitable for fluorescent quantitative PCR detection of transgenic components of high-oil crops. The kit comprises a lysis solution, a precipitation solution, a neutralization solution and an elution solution. The application can be completed within 15 minutes, effectively removes PCR inhibitors such as oil and protein, is specifically used for high-oil crops, and is a low-cost, safe and non-toxic nucleic acid rapid extraction method, and meets the requirement of on-site rapid detection of transgenic crops.

The application belongs to the field of agricultural biotechnology, and discloses a database construction and analysis method for soybean transgenic crops, which comprises the following steps: obtaining soybean high-throughput sequencing original data and metadata, performing soybean specificity quality control, storing in a database sub-database according to a scene label, and generating standardized sub-database data; identifying the priority of each sub-database scene, allocating hardware according to a priority-resource matching rule, generating resource configuration records, synchronously executing multi-dimensional feature extraction, and generating a multi-dimensional feature dataset; dynamically adjusting the feature importance degree according to the scene, performing feature collaborative analysis, combining an enhanced decision knowledge base to retrieve and match results and solving feature conflicts, and generating a dynamic confidence identification report; extracting key feature data and identification process information to generate a hash value, writing the hash value into a consortium chain, and associating a soybean sample ID to form a digital traceability certificate; dynamically optimizing the feature importance degree, the enhanced decision knowledge base and the database sub-database rule, and forming a full-link iteration.

The invention provides a crop allergen dynamic baseline construction and trusted sharing system based on a multi-dimensional heterogeneous data cube, and the system comprises a collection layer which is used for collecting physical information metadata of a to-be-detected sample based on an agricultural environment monitoring terminal, the method comprises the following steps: performing physical detection on a to-be-detected sample through a high-throughput sequencing terminal to generate original mass spectrum data and transcriptome data, and calling meteorological metadata of a region where the to-be-detected sample is located through a third-party meteorological server; the core processing layer is used for generating a dynamic baseline by adopting a central processing server cluster based on the physical information metadata, the original mass spectrum data, the transcriptome data and the meteorological metadata; and the application layer is used for checking the dynamic baseline based on the user query terminal and checking the GLP log based on the checking and auditing terminal. By constructing the multi-dimensional heterogeneous data cube and the dynamic baseline model, standardized data calculation service is provided for safety evaluation of transgenic crops and biotechnology products.

This invention relates to the field of plant transgenic detection technology, and discloses a method for rapid batch detection of transgenic crops, comprising: Step 1, placing centrifuge tubes containing crop samples to be tested in an array into a multi-well plate to complete batch plate arrangement of samples; Step 2, performing overall mechanized processing based on the multi-well plate, so that the crop samples in the centrifuge tubes are simultaneously ground into powder under mechanical force. This integrated operation process, which places independent centrifuge tubes in an array within a multi-well plate and uses a tissue homogenizer, batch liquid addition equipment, and a centrifuge to simultaneously process all samples, achieves a significant improvement in detection throughput and efficiency. Compared to existing technologies that rely on manual, one-by-one operation, this method overcomes the shortcomings of low detection efficiency and the inability to simultaneously process multiple samples, thus failing to meet the needs of large-scale crop sample screening.

The invention provides an insect-resistant and herbicide-resistant gene of corn and application of the insect-resistant and herbicide-resistant gene to cultivation of insect-resistant and herbicide-resistant corn, and belongs to the field of plant disease resistance. Experiments prove that the expression vector for simultaneously expressing the Cry1Ab, Cry2Ab and cp4-epsps genes is constructed for the first time, transgenic crops obtained by transforming crops with the vector can simultaneously resist Lepidoptera pests such as ostrinia nubilalis and cotton bollworm and have good glyphosate resistance, the insect-resistant and herbicide-resistant application of the transgenic crops is widened, and the transgenic crops have broad application prospects. The method has a wide application prospect in corn genetic breeding improvement.

Provided in the present invention is a hypermorphic allele ETD1 against rice blast, wherein the ETD1 gene has a nucleotide sequence as set forth in SEQ ID NO: 1, and a cDNA sequence thereof is as set forth in SEQ ID NO: 2. Further provided is an encoded protein of the gene, wherein the protein has an amino acid sequence as set forth in SEQ ID NO: 3. The gene is a hypermorphic allele of OsCNGC13, which exhibits enhanced calcium-ion transport capacity and has the function of significantly accelerating the influx of calcium ions into cells. Therefore, the normal expression or overexpression of the protein in a gene knockout plant can enhance resistance of rice, and thus the protein has a positive regulatory effect on rice blast resistance. The present invention can be used for the rapid identification of transgenic crops harboring ETD1 mutant genes, thereby improving the efficiency of molecular biological breeding.

This invention belongs to the field of genetic engineering pest control technology, specifically relating to an insect-resistant protein Cry166 that enhances plant resistance to pests and its applications. The amino acid sequence of the insect-resistant protein Cry166 is shown in SEQ ID NO:1, and the application is in plant pest control. After expression in plants, the insect-resistant protein Cry166 provided by this invention significantly enhances the resistance of transgenic crops containing it to the target pest, the fall armyworm. It also expands the insect resistance spectrum of this protein, solving the problems of limited insect resistance mechanisms, narrow insect resistance spectrum, and easy development of resistance in insects in existing technologies. Furthermore, it improves the breeding efficiency of insect-resistant plants to a certain extent and reduces breeding costs.

The present invention belongs to the field of plant genetic engineering technology and specifically provides the use of the GhGLK1 protein and its encoding gene in regulating cotton callus growth and differentiation. The protein meets the following conditions: B1) a protein with an amino acid sequence of SEQ ID NO. 1; B2) a fusion protein with the same function obtained by attaching a tag to the N-terminus and / or C-terminus of B1). GhGLK1 After the gene was cloned, overexpression vectors and CRISPR vectors were constructed, they were transformed into cotton to obtain overexpression plants and knockout plants. GhGLK1 Overexpression plants can significantly improve callus growth and differentiation, greatly shortening the transformation cycle of transgenic crops. This is of great significance for breaking the dependence on the genotype of the recipient material and accelerating the cycle of plant transformation and regeneration.

Various examples relate to a transgenic crop plant where a sequence of a cis-regulatory element in the promoter of certain genes is genomically changed in order to alter the flowering time or flowering rate of the plant. In one example, the cis-regulatory element in the promoter of such certain genes may be modified from TGAATG(A / T) (A / T / C) to TGAGGG(A / T) (A / T / C). The certain genes may be genes that encode SbEhd1, OsFTL10 / FT8, SbFT10, ZmELF3.2, or combinations thereof. And, the crop plant can be for example, sorghum, maize or rice. In another example, the present transgenic crop plant is a progeny plant. In yet another example, flowering time of the transgenic crop plant is regulated by a method that includes changing the sequence of the cis-regulatory element in the promoter of certain genes.

The invention belongs to the technical field of gene engineering pest control, and particularly relates to an insect-resistant protein Cry166 capable of improving insect pest resistance of plants and application of the insect-resistant protein Cry166, the amino acid sequence of the insect-resistant protein Cry166 is shown as SEQ ID NO: 1, and the application refers to application in the aspect of plant pest control. After the insect-resistant protein Cry166 provided by the invention is expressed in a plant body, the resistance of a target pest spodoptera frugiperda of a transgenic crop containing the insect-resistant protein Cry166 is greatly improved, meanwhile, the insect-resistant spectrum of the insect-resistant protein is expanded, and the problems that in the prior art, the insect-resistant mechanism is few, the insect-resistant spectrum is narrow, insects easily generate resistance and the like are solved; the breeding efficiency of insect-resistant plants is improved at a certain level, and the breeding cost is reduced.

The present invention relates to a method for the relative quantification of GM crops using digital PCR without using certified reference materials (CRMs). More specifically, the present invention relates to a primer and probe set composition for digital PCR for the relative quantification of transgenes in GM crops without using CRMs, which comprises a first primer and probe set capable of specifically detecting genes present in a single copy or in 2-3 copies within the genome of GM (genetically modified) crops; and a second primer and probe set capable of specifically detecting transgenes in GM crops, and uses thereof.

The invention relates to a single-chain antibody for recognizing Vip3Aa toxin of bacillus thuringiensis and application of the single-chain antibody. The single-chain antibody is constructed by taking a mouse hybridoma cell strain secreting a monoclonal antibody for resisting the Vip3Aa toxin as a template; the nucleotide sequence and the amino acid sequence are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2; the single-chain antibody can be used as a detection antibody, is applied to detection of Vip3Aa toxin by a double-sandwich ELISA (enzyme-linked immuno sorbent assay) method, is high in sensitivity and specificity, and can be used for rapid large-scale screening of Vip3Aa toxin in transgenic crops.

The invention discloses a method for evaluating the influence of insect-resistant transgenic crops on the diversity of enteric microorganisms of natural enemy insects based on a 16S rRNA high-throughput sequencing technology. The method comprises the following steps: (1) space-time gradient sampling: collecting natural enemy insect samples according to a crop growth cycle in a transgenic crop planting area or a laboratory simulation environment; (2) sequencing intestinal microorganisms: extracting DNA (deoxyribonucleic acid) of intestinal contents, amplifying a V3-V4 region of the 16S rRNA gene through a universal primer, and sequencing; (3) bioinformatics analysis: an alpha diversity (Shannon, Chao1 index) and a beta diversity (PCoA) are calculated, and the microflora structure difference is analyzed; and (4) screening of core bacteria: identifying the core bacteria significantly related to the health indexes (survival rate and egg laying amount) of the natural enemy insects based on Spearman correlation analysis (rgt, 0.7, plt, 0.05), and establishing a prediction model to evaluate ecological risks. According to the method, the non-target effect of the transgenic crop is quantified through the dynamic change of the microbiome, and a new strategy is provided for ecological safety evaluation.

The present invention discloses a method for producing a modified crop plant with enhanced tolerance to abiotic stress-induced oxidative stress. The method comprises introducing at least one genomic modification in the endogenous ATR7 gene using targeted genome editing techniques. A modified crop plant exhibiting improved resilience to oxidative stress conditions induced by abiotic factors, due to the disruption or knockout of the ATR7 gene is also disclosed.

The invention discloses a gene related to synthesis of amygdalin from crop seed endosperm and application of the gene, and belongs to the technical field of gene engineering. According to the invention, gene sequences of CYP79D16, CYP71AN24, UGT94AF3 and UGT94AF2 are optimized, CYP79D16 and CYP71AN24 are connected by using a linker peptide I to obtain a fusion gene I, and the fusion gene I is fused with a breeding crop endosperm strong expression promoter to construct a gene expression cassette I; connecting UGT94AF3 and UGT94AF2 by using a connecting peptide II to obtain a fusion gene II, and fusing the fusion gene II with a breeding crop endosperm strong expression promoter to construct a gene expression cassette II; transferring the gene expression cassette into an expression vector to construct a recombinant vector, and introducing the recombinant vector into a plant to obtain a transgenic crop. According to the invention, synthesis of amygdalin in crop seed endosperm is realized, the storability of crop seeds is improved, and the product can be used as a raw material for producing amygdalin, and has guiding significance and production application value for crop breeding.

The present invention discloses a method and system for dynamically identifying risks of biotechnology products based on multi-indicator fusion, which relates to the field of biosafety management technology, including: S1, obtaining experimental data and environmental data from various stages of the life cycle of genetically modified crops, constructing a dynamic data set including molecular structure sequences, functional performance indicators and ecological impact factors, and extracting the mutation frequency of molecular structure sequences over time, abnormal fluctuations in functional performance indicators and dynamic change trends of ecological impact factors; S2, based on the mutation frequency of molecular structure sequences and abnormal fluctuations in functional performance indicators, extracting nucleotide substitution patterns and functional abnormality data sets of gene editing products in the research and development stage and the application stage, and determining the correlation pattern between nucleotide substitution patterns and functional abnormalities; this method and system for dynamically identifying risks of biotechnology products based on multi-indicator fusion realizes accurate prediction and dynamic management of ecological risks of genetically modified crops, and significantly improves the safety of environmental release.

The invention discloses a cyhalofop-butyl-resistant digitaria sanguinalis MYB transcription factor gene and application thereof, and relates to the field of plant genetic engineering, and the expression of the gene in a resistant digitaria sanguinalis biotype is remarkably up-regulated. After a plant expression vector is constructed and corn is transformed, after 11.25 g a.i. Ha-1 cyhalofop-butyl is sprayed on a transgenic corn plant in a 3-4 leaf period, the pesticide resistance and the survival rate are obviously enhanced, and the transgenic corn plant is obviously superior to a wild type. The invention proves that the MYB gene can endow plants with cyhalofop-butyl resistance, provides a new gene resource for cultivating transgenic crops resisting acetyl-CoA carboxylase (ACCase) inhibitor herbicides, and has important application value.

The invention discloses a method for controllably removing an exogenous vector skeleton and a selection marker. According to the method, a novel transformation vector sequence containing an isolated expression cassette sequence composed of a non-autonomous transposon and a target gene is designed, and the isolated expression cassette is inserted into a corn kernel purple aleurone layer marker gene sequence to form a target gene expression cassette transposition detection system. Meanwhile, a fluorescent protein coding gene specifically expressed by a grain aleurone layer and a selection marker gene sequence are utilized to form a vector skeleton detection system. According to the method, a plurality of new ultra-pure transformant events can be obtained from one initial transformation event, the safety evaluation cost and time are saved, the number of transformation events is increased, and the method has great value for accelerating and promoting industrialization of transgenic crops.

The invention belongs to the technical field of plant biology, and particularly relates to an amaranthus retroflexus ArCYP81Q32 gene and application thereof. The invention discovers that amaranthus retroflexus ArCYP81Q32 participates in the degradation of a metabolic detoxification protoporphyrinogen oxidase (PPO) inhibitor herbicide fluoroglycofen-ethyl in a plant body. The relationship between the amaranthus retroflexus ArCYP81Q32 gene and fluoroglycofen-ethyl resistance is disclosed for the first time, whether a plant material generates metabolic resistance to herbicides or not can be identified by detecting the expression quantity of the amaranthus retroflexus ArCYP81Q32 gene, and the method has guiding significance on timely discovery of resistance, guidance of scientific pesticide application in farmland and alleviation of further spreading of pesticide resistance. The amaranthus retroflexus ArCYP81Q32 gene detoxifies protoporphyrinogen oxidase inhibitor herbicides through metabolism, and also provides a new gene resource for developing transgenic crops resisting the herbicides.

The present application discloses a polynucleotide, a method and a kit for detecting transgenic crops. Specifically, the present application discloses a polynucleotide construct pLW10 and its matching primer probe sequence which can be used as a standard molecule for real-time fluorescent PCR detection of transgenic crops. The plasmid standard molecule of the present application solves the problem of lack of standard substance in real-time fluorescent PCR detection of transgenic crops, ensures the comparability of the detection results of real-time fluorescent PCR method of transgenic crops, and provides a reliable quality control method for real-time fluorescent PCR method detection of transgenic crops.

The application discloses a herbicide-resistant protein GAT hybridoma cell strain, an antibody produced by the hybridoma cell strain and application of the hybridoma cell strain, and the hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No.46333.A preparation method of the hybridoma cell strain comprises the following steps: a) purifying a GAT recombinant protein obtained by prokaryotic expression; b) immunizing animals: using the GAT recombinant protein as an antigen to immunize BALB / c mice; c) cell fusion: collecting spleen cells of the immunized BALB / c mice and fusing the spleen cells with SP2 / 0 cells; d) cell strain establishment: performing subcloning by a limited dilution method, performing ELISA detection on the subcloning for 5-7 days, and screening the hybridoma cell strain stably secreting positive antibodies until the hybridoma cell strain is expanded, cultured and preserved again.The monoclonal antibody secreted by the hybridoma cell strain lays a foundation for realizing detection of the herbicide-resistant protein GAT in a transgenic crop, thereby supporting the development, seed production, seed preservation and guarantee of the transgenic crop, and stably promoting industrialization of the transgenic crop and sustainable application of related transformants. gat The application discloses a herbicide-resistant protein GAT hybridoma cell strain, an antibody produced by the hybridoma cell strain and application of the hybridoma cell strain, and the hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No.46333.A preparation method of the hybridoma cell strain comprises the following steps: a) purifying a GAT recombinant protein obtained by prokaryotic expression; b) immunizing animals: using the GAT recombinant protein as an antigen to immunize BALB / c mice; c) cell fusion: collecting spleen cells of the immunized BALB / c mice and fusing the spleen cells with SP2 / 0 cells; d) cell strain establishment: performing subcloning by a limited dilution method, performing ELISA detection on the subcloning for 5-7 days, and screening the hybridoma cell strain stably secreting positive antibodies until the hybridoma cell strain is expanded, cultured and preserved again.The monoclonal antibody secreted by the hybridoma cell strain lays a foundation for realizing detection of the herbicide-resistant protein GAT in a transgenic crop, thereby supporting the development, seed production, seed preservation and guarantee of the transgenic crop, and stably promoting industrialization of the transgenic crop and sustainable application of related transformants. gat The application discloses a herbicide-resistant protein GAT hybridoma cell

This invention discloses a hybridoma cell line containing the herbicide-resistant protein GAT, its produced antibody, and its applications. The hybridoma cell line is deposited at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC No. 46332. The preparation method includes: a) purifying prokaryotic expression to obtain recombinant GAT protein; b) immunizing animals: using the recombinant GAT protein as an antigen to immunize BALB / c mice; c) cell fusion: collecting spleen cells from immunized BALB / c mice and fusing them with SP2 / 0 cells; d) cell line establishment: subcloning using the limiting dilution method, performing ELISA detection on subclones after 5-7 days, until a stable hybridoma cell line secreting positive antibodies is selected for expansion, reculturing, and preservation. The monoclonal antibody secreted by the hybridoma cell line of this invention lays the foundation for the detection of the herbicide-resistant protein GAT in transgenic crops, thus supporting the development of transgenic crops. street The development, seed production, and preservation of genetically modified crops have ensured the survival of genetically modified organisms (GMOs). street The industrialization of genetically modified crops is progressing steadily, and the sustainable application of related transformants is advancing.

The present application belongs to the technical field of biological detection, and discloses a transgenic crop intelligent identification method based on high-throughput sequencing, which comprises the following steps: obtaining multi-platform high-throughput sequencing original data and sample metadata, evaluating quality in a platform-by-platform manner, dividing according to sample block rules, and generating a quality-controlled data block set; distributing the data block to multiple parallel computing nodes, synchronously executing technical detection to obtain technical fact segments, aggregating and supplementing batch statistical information, and generating a technical fact report; matching a judgment rule set, performing parallel judgment, triggering expert review for boundary cases, and then integrating to generate a supervision judgment conclusion set; extracting all sample original sequencing reads, generating a unique hash for each read, constructing a processing track chain, integrating metadata and visualized evidence, generating a credible identification report and dividing different scenario versions, and performing targeted distribution; extracting identification cases for mining and clustering, and generating optimization suggestions to feed back to a quality control rule library and a judgment rule library.

The invention discloses a wheat endogenous high-activity TaUbi promoter and an application of the wheat endogenous high-activity TaUbi promoter. The nucleotide sequence of the TaUbi promoter is as shown in SEQ ID No. 1. Experiments prove that the TaUbi promoter has obviously improved transcriptional activity and endogenous stability, and can greatly improve the expression level of a target gene; the TaUbi promoter drives the expression of Cas9 protein capable of recognizing a specific DNA sequence and cutting, so that the protein yield can be remarkably increased, and the gene editing efficiency is improved. The method has the outstanding advantages of high expression activity, good species specificity, strong stability and the like, is suitable for cultivation of conventional transgenic crops, can be compatible with various gene editing systems such as CRISPR / Cas9 and the like, provides an efficient and reliable multifunctional tool platform for wheat genetic improvement, and can powerfully promote the development of wheat molecular breeding. The method has an important application value.

The present application relates to the field of biotechnology, in particular to a method for heterologous synthesis of dammarane-type ginsenosides in the endosperm of crop seeds, a vector, a plant and application. Through modular design, a core gene module driven by an endosperm-specific promoter is assembled in the T-DNA region of the vector, and a downstream glycosylation or hydroxylation-glycosylation modification module is configured to realize the directional synthesis of PPD-type and PPT-type ginsenosides, respectively. The transgenic crops obtained by using the vector can specifically accumulate target ginsenosides in the endosperm. The obtained ginseng rice shows clear anti-tumor activity in in vitro tumor cell experiments and tumor-bearing mouse models. The present application provides a new strategy for low-cost production of high-value natural products, and opens up a new direction for the application of functional crops in the field of medicine.

The invention belongs to the technical field of biological safety evaluation, and particularly relates to a method for evaluating non-target biological safety of transgenic insect-resistant corn in soil and application of the method. According to the method, an artificial soil method is adopted to simulate natural exposure conditions, and leaves of transgenic insect-resistant corn and non-transgenic control corn in the elongation stage are freeze-dried and then added into artificial soil for culturing eisenia fetida. After in-vivo DNA of eisenia fetida in different culture periods is extracted respectively, PCR amplification is carried out on bacterial 16S rRNA genes and fungus ITS regions, a library is constructed, and amplicon sequencing is carried out. The safety of the transgenic insect-resistant corn to a non-target organism eisenia fetida is comprehensively evaluated by analyzing the change of the diversity of Alpha and Beta of microorganisms in eisenia fetida. According to the invention, a scientific, accurate and efficient evaluation method is successfully established, and the method has important significance for perfecting an evaluation system for non-target biological safety of transgenic crops.

The invention provides a siRNA nucleic acid pesticide for controlling magnaporthe oryzae by targeting a DICER2 gene, and relates to the technical field of biology. The siRNA molecule can delay and inhibit the formation of magnaporthe oryzae appressorium, so that the pathogenicity of magnaporthe oryzae is reduced, and a new method for preventing and treating magnaporthe oryzae is provided; when the composition is combined with tricyclazole or clotrimazole for use, a synergistic effect is achieved. The siRNA molecule of the targeted DICER2 gene is prepared into a nucleic acid pesticide, and the nucleic acid pesticide is sprayed on leaves of rice, so that leaf rice blast scab and scab area are effectively reduced, the siRNA molecule can be further used for preventing and treating rice blast, and the tedious process of cultivating transgenic crops and the worry of consumer groups can be avoided.

The invention discloses a cyhalofop-butyl-resistant digitaria calorata MYB transcription factor gene and application thereof, and relates to the field of plant genetic engineering, and the expression of the gene in a resistant digitaria calorata biological type is remarkably up-regulated. After a plant expression vector is constructed and corn is transformed, after 11.25 g a.i. Ha-1 cyhalofop-butyl is sprayed on a transgenic corn plant in a 2-leaf stage, the pesticide resistance and the survival rate are obviously enhanced, and the transgenic corn plant is obviously superior to a wild type. The invention proves that the MYB gene can endow plants with resistance to acetyl-coenzyme A carboxylase (ACCase) inhibitor herbicides such as cyhalofop-butyl and the like, provides a new gene resource for cultivating transgenic crops resisting the acetyl-coenzyme A carboxylase (ACCase) inhibitor herbicides, and has important application value.