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90 results about "Resistance development" patented technology

Method and special primer of method for fast identifying plutella xylostella ryanodine receptor gene mutation

InactiveCN102952889ATimely adjustment of prevention and control strategiesHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceOpen reading frame
The invention discloses a method and a special primer of the method for fast identifying the plutella xylostella ryanodine receptor gene mutation. The invention provides a specific primer combination, which consists of a single chain DNA (deoxyribonucleic acid) molecule shown as a sequence 1 of a sequence table, a single chain DNA molecule shown as a sequence 2 of the sequence table and a single-chain DNA molecule shown as a sequence 3 of the sequence table. The specific primer combination can be used for the following (a) or (b): (a) the specific primer combination can be used for identifying whether plutella xylostella generates the G4899E mutation of the ryanodine receptor gene or not, and the G4899E mutation of the ryanodine receptor gene refers to that an open reading frame of the ryanodine receptor gene is mutated into A from G in the 14696 locus nucleotide of the 5' tail end; and (b) the specific primer combination is used for the auxiliary identification of the resistance of plutella xylostella on bisamide pesticides. The specific primer combination and the application of the specific primer combination have the important significance in the aspects of monitoring the resistance gene frequency of plutella xylostella field populations on the bisamide pesticides and the bisamide pesticide resistance generation and development trend of the plutella xylostella field populations, timely regulating the plutella xylostella preventing and control strategy, guiding the reasonable pesticide use and delaying the resistance development.
Owner:CHINA AGRI UNIV

Selenium-rich plant-derived synergistic biological compound capsule fertilizer and preparation method thereof

The invention belongs to the field of biological fertilizer, and particularly discloses selenium-rich plant-derived synergistic biological compound capsule fertilizer and a preparation method thereof. The selenium-rich plant-derived synergistic biological compound capsule fertilizer mainly comprises inorganic fertilizer, organic fertilizer and microbial fertilizer. The selenium-rich plant-derived synergistic biological compound capsule fertilizer is prepared from the following components in parts by weight: 20 to 25 parts of pepper, 10 to 15 parts of tobacco leaves, 15 to 20 parts of allicin, 10 to 15 parts of Dongguo, 20 to 25 parts of mint and the like. The selenium-rich plant-derived synergistic biological compound capsule fertilizer provides phoxim and triazophos, the release of active ingredients of a pesticide can be reasonably regulated, so that the pesticide effect can be improved, the effect lasting period can be prolonged, and the environmental pollution can be reduced; by compounding the phoxim and the triazophos, a synertistic effect is obvious; by organically combining the pepper, the tobacco leaves, the allicin, the Dongguo, the mint and the like, the pesticide effect of a pesticide composition can be maximized; and the raw materials are relatively easy to obtain, the production cost is low, the resistance development is slowed down, and the service life of the pesticide is prolonged.
Owner:CHANGSHU HENGRUI KECHUANG ENTERPRISE MANAGEMENT CO LTD
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