The invention discloses a plant-derived pesticide and its preparation method and use and belongs to the technical field of pesticide preparation. The plant-derived pesticide is prepared from tobacco, common threewingnut root, capsicum, rhizome of Chinese monkshood, pyrethrum, Azadirachta indica, tea leaves, patrinia herb, bitter herbs, wormwood, ash bark, radix sophorae flavescentis, Euphorbia songarica Boiss, Derris fordii Oliv., Chinese prickly ash, purslane, mandala, Anabasis Aphylla L., leaves of Salix babylonica, leaves of Pterocarya stenoptera and water. The plant-derived pesticide is prepared by soup extraction, condensation, drying, crushing, sieving and disinfection. The plant-derived pesticide is nontoxic and harmless, does not produce residues, is harmless, is environmentally friendly, does not pollute the environment, can effectively prevent and control injurious insects such as yellow spider, arrowhead scales, leaf roller moth and fruit fly, has a one-step killing rate of 95.41% or more, has no drug resistance and can be used for 4-6 years.