49 results about "Jasmonate" patented technology

Aspects of the present invention concern tobacco having reduced nicotine and nitrosamines, which is used to manufacture consumer tobacco products, and methods to produce such tobacco. More specifically, embodiments include methods to reduce nicotine and nitrosamines in tobacco crops by applying auxin, auxin analogs, and jasmonate antagonists during the cultivation of such tobacco crops. Tobacco generated using said methods and consumer tobacco products obtained therefrom are also embodiments.

The invention discloses a gene capable of reducing nicotine content of tobacco and an application thereof. The gene is a tobacco JAZ protein gene which is named as NtJAZ1, and protein encoded by the gene disclosed by the invention contains a typical TIFY structural domain and a jas structural domain and is induced and expressed by jasmonate. An RNAi (Ribonucleic Acid Interfere) transgenic carrier of the NtJAZ1 gene is constructed, and the tobacco is converted, so that expression of the NtJAZ1 in tobacco cells is successfully inhibited, and the nicotine content of the obtained transgenic tobacco cell line is obviously reduced compared with that of a wild type tobacco cell line. The gene disclosed by the invention provides an important basis and a technical support for low-nicotine-content tobacco breeding.

The invention discloses a gene capable of reducing nicotine content of tobacco and an application thereof. The gene is a tobacco JAZ protein gene which is named as NtJAZ3, and protein encoded by the gene disclosed by the invention contains a typical TIFY structural domain and a jas structural domain and is induced and expressed by jasmonate. An RNAi (Ribonucleic Acid Interfere) transgenic carrier of the NtJAZ3 gene is constructed, and the tobacco is converted, so that expression of the NtJAZ3 in tobacco cells is successfully inhibited, and the nicotine content of the obtained transgenic tobacco cell line is obviously reduced compared with that of a wild type tobacco cell line. The gene disclosed by the invention provides an important basis and a technical support for low-nicotine-content tobacco breeding.

The invention discloses a highly effective revulsion induction method for pinellia tuber excised tuber comprising the steps of, using successive transfer culture base for transferred culture, using root-producing culture medium for root-producing culture, using in vitro tuber induction culture medium for in vitro tuber induced culturing, wherein the successive transfer culture base comprises MS minimum medium, cytokinin, 1-naphthalene acetic acid, agar powder and cane sugar, the root-producing culture base comprises MS minimum medium, 1-naphthalene acetic acid, activated charcoal, agar powder, cane sugar, the in vitro tuber induction culture medium comprises MS minimum medium, gibberellin, abscisic acid, propyl dihydro-jasmonate, cane sugar, Paclobutrazol, cytokinin, activated charcoal and agar powder.

The invention belongs to the field of biological engineering, and particularly relates to a method for improving the content of betulin and oleanolic acid in a birch cell by utilizing MeJA (methyl-jasmonate) and SA (salicyl acid), which comprises the following steps of: inoculating a birch tissure culture seedling stem to NT + 0.1 mg / L of 6-BA and 0.01 mg / L of TDZ culture mediums to induce birch callus, and obtaining a birch suspension cell with high content of total triterpenes through multi-time subculture, screening and suspension culture; inoculating the screened cell into the same liquid culture medium, wherein the inoculation amount is 3g / 100ml; adding 100mu Mum / L of MeJA and 50mg / L of SA when the shake culture is performed at the seventh day; after 24-48h, obtaining the product; and detecting the content of total triterpenes, betulin and oleanolic acid in the cell. Through the comparison of the two kinds of processing methods, the content of total triterpenes is improved by 21.6-31.7%, the content of oleanolic acid is improved by 277.4-369.34%, and the content of betulin is improved by 44.42-179.58%. The application of the invention provides a new path for solving the problem of shortage of natural plant drug resources and realizing large-scale industrial production of betulin and oleanolic acid drugs.

A formulation, composition or combination of substances comprising jasmonate for modulating melatonin production and / or calcification of a pineal gland and / or modulation and / or treatment of age-related neurodegeneration in a subject, particularly in a mammalian subject and more particularly in a human subject and use of jasmonate for modulating melatonin production and / or calcification of a pineal gland and / or modulation and / or treatment of age-related neurodegeneration is provided.

The invention discloses an inhibiting factor JAZ (Jasmonate ZIM domain) gene-SmJAZ3 and corresponding protein sequence thereof in salviae miltiorrhizae jasmonic acid signal pathway. The SmJAZ3 gene has a nucleotide sequence shown in SEQ ID NO.1. Gene encoded protein has an amino acid sequence shown in SEQ ID No.2. An over-expression and antisense inhibitory carrier is constructed, genetic transformation is conducted on salvia miltiorrhiza hairy roots, and tanshinone content in salvia miltiorrhiza is obviously affected. The SmJAZ3 is closely related to tanshinone, quality improvement of radix salviae miltiorrhizae is facilitated, and a new action target and research idea are provided for solving the problem of medicine source shortage of the tanshinone.

The invention belongs to the technical field of rice plantation, and discloses a breeding and seed production method for hybrid rice. The method utilizes the measures including performing field layoutof male and female parents, promoting glume opening by a sterile line, collecting pollen of the male parent, spraying, and the like; wherein concentration plantation is separately performed on the male and female parents, seedling raising is performed by utilizing machine transplantation in order to remove impurities; different restoration systems are isolated by distance or flowering periods, and the pollen of the male parent is mechanically collected and preserved at ultralow temperature; when the sterile line shows 30% of ears, "920" is utilized to promote earing, and when the sterile lineshows 50% of the ears, concentration glume opening is promoted by utilizing jasmonates or [alpha]-linolenic acid substances; the pollen of the male parent is mechanically collected and preserved at ultralow temperature, and the pollen is sprayed by a unmanned aerial vehicle; and hybrid seeds are harvested by a harvester. The method contributes to mechanization of planting and transplanting linksin breeding and seed production processes, and avoids generation of mechanical mixing when the hybrid seeds are harvested; the problem that the flowering periods of the male and female parents do notmeet is avoided; and the sterile line promotes the concentration glume opening through combination of spraying the pollen by the unmanned aerial vehicle, and so, the labor intensity is reduced, and the efficiency of breeding and seed production is improved.

The invention relates to the field of daily chemical essences, and particularly discloses a daily chemical essence with flower fragrance and a preparation method thereof. The daily chemical essence with the flower fragrance comprises 0.6%-1.4% of B ionone. 0.2%-0.7% of n-amyl salicylate; 0.05%-0.15% of cis-jasmonic ketone; 0.5%-1.5% of convallaldehyde; 6%-10% of benzyl acetate; 1%-3% of methyl dihydrojasmonate; 0.1%-0.5% of methyl benzoate; 0.5%-1.5% of indole; 0.1%-0.3% of leaf alcohol; 0.1%-0.5% of salicylic acid leaf alcohol ester; 1%-3% of phenethyl alcohol; and the balance of dipropyleneglycol, wherein the total mass percentage is 100%. The preparation method comprises the following steps: mixing B-ionone, n-amyl salicylate, cis-jasmonate, convaleraldehyde, benzyl acetate, methyl dihydrojasmonate, methyl benzoate, indole, leaf alcohol, geraniol, leaf alcohol salicylate, phenethyl alcohol and dipropylene glycol in proportion, heating to 45-55 DEG C while stirring, and standing for45-55 minutes to obtain the daily chemical essence with flower fragrance. The daily chemical essence with the flower fragrance has the advantages that the fragrance lasting time of the daily chemicalessence is prolonged, and the cost is relatively low.

The invention belongs to the technical field of plant genetic engineering, and in particular relates to over-expression GhJAZ1 genes capable of enhancing low temperature stress resistance of plants. JAZ (JASMONATE-ZIM DOMAIN) family protein genes GhJAZ1 are cloned from cotton, a cDNA sequence of the genes GhJAZ1 is shown in SEQ ID NO: 1, and the coded protein sequence is shown in SEQ ID NO: 2. An over-expression vector of the genes is constructed, and a transgenic plant is obtained according to a genetic transformation method. The cloned genes in the invention are proved to be capable of improving the tolerance of the cotton on low temperature stress.

The invention discloses a method for creating a plant male sterile line. The method includes: lowering content of protein shown as a sequence 3 in a receiver plant or inhibiting activity of the protein in the receiver plant or knocking out coding gene of the protein in the receiver plant or reducing expression of the coding gene of the protein in the receiver plant to obtain the plant sterile line. Fertility of the plant sterile line obtained by the method can be restored by applying jasmonate or derivative thereof, and the method is not limited by genetic background, avoids negative effect ofsterile cytoplasm, is of important significance in expanding plant sterile line source, simplifying breeding process, accelerating plant breeding speed and lowering breeding cost and can promote quick development of different plant crossbreeding.

This invention involves a cis-dihydrojasmone acid methyl ester synthetic method. The existing multi-step synthesis method is low yield and product content. This invention takes ordinary dihydrojasmone acid methyl ester as raw material, by enolase reaction and halogenation reaction, to dehalogenate to prepare double bonds jasmonate methyl ester; finally, under the solvent-free state through hydrogenation to obtain cis-dihydrojasmone acid methyl ester.

The present invention relates to novel jasmonate derivatives, methods for their preparation, pharmaceutical compositions including such compounds, and methods of using these compounds and compositions, especially as chemotherapeutic agents for prevention and treatment of cancers.

A parahormone scutellarin promoting plant root elongation, and root elongation effect inhibition of antagonism auxin substances 2,4-D and antagonism methyl jasmonate is provided, and an application of scutellarin in preparation of parahormones for promoting the plant root elongation, and the root elongation effect inhibition of the antagonism auxin substances 2,4-D and the antagonism methyl jasmonate is also provided. The plant root elongation promotion effect of the plant secondary metabolite scutellarin is first disclosed, scutellarin mutually antagonizes with the two phytohormone 2,4-D andmethyl jasmonate having the root elongation inhibition effect, and the antagonism effect is dose-dependent, so the plant root elongation effect of scutellarin is a parahormone effect. Above application is a first application of scutellarin in the plant field, so a new field is displayed for the application of scutellarin, thereby the excavation of the value of scutellarin is promoted.

The present invention provides a method for efficiently producing a 2-alkyl-2-cyclopentenone as well as a method for producing a jasmonate derivative by using the same. That is, in the present invention, the compound (2) is obtained by reacting an amine and a hydrogen halide with the compound (1) at a specific ratio to carry out isomerization reaction or by reacting a catalyst comprising an amine and a hydrogen halide with the compound (3) to carry out dehydration-isomerization reaction. Further, this compound (2) is reacted with a malonic acid diester and then reacted with water to obtain a jasmonate derivative (5): wherein each of R1 and R2 represents H, a C1-8 alkyl group or the like, and R3 represents a C1-3 alkyl group.

The invention discloses a tobacco JAZ protein gene and application thereof in reducing tobacco nicotine content. The gene is a tobacco JAZ protein gene and is named as NtJAZ7, the protein coded with the gene has a typical TIFY structure field and a jas structure field, and the gene is subjected to induced expression of jasmonate. Through constructing an RNAi gene transfer vector of the NtJAZ7 gene, and transforming the tobacco, the expression of NtJAZ7 in tobacco cells is successively inhibited, and the nicotine content of the obtained transgenic tobacco cell line is obviously reduced compared with the wild type tobacco. The invention provides an important scientific basis and a technological support for breeding low-nicotine content tobacco.

The invention discloses a method for accelerating the germination of camellia oleifera seeds with sawdust. The method includes the steps of: (1) screening of camellia oleifera seeds; (2) seed soakingwith water and germination accelerating liquid respectively; (3) preparation of sawdust; and (4) germination acceleration. The germination accelerating liquid comprises the following components by weight: 0.2-0.5 part of brassinosteroid, 0.005-0.01 part of gibberellin, 0.002-0.005 part of thiophanate-methyl, 8-10 parts of phosphatotri(hydroxymethyl)methylglycine jasmonate, and 95-100 parts of water. The method for accelerating the germination of camellia oleifera seeds with sawdust disclosed by the invention has the characteristics of simple operation, good germination accelerating effect, high bud rate and survival rate of seeds, high seedling emergence rate of plants, good regularity and later growth capacity, can effectively bring the camellia oleifera sowing time ahead of schedule, andreach the purposes of early sowing and early seedling emergence, and yield increase, thereby having good economic, social and ecological benefits.

The present invention relates to methods for obtaining a transgenic plant that overproduces jasmonic acid, and optionally OPDA, a jasmonic acid precursor. Said methods include transforming the plant using a nucleic acid encoding ORA47. The accumulation of jasmonic acid confers in particular to the transformed plant an improved resistance to pathogenic agents. The transgenic plants obtained may also be used for the production of pharmaceutically important secondary metabolites whose synthesis is induced by jasmonates.

The invention discloses an Escherichia coli strain reformed by gene engineering, and applications thereof. According to the present invention, engineering bacteria for preventing and controlling smutis constructed by using the applications of methyl jasmonate in prevention and controlling of smut, and recipient bacteria over-express methyl jasmonate transferase; the metabolite of the engineeringbacteria is methyl jasmonate, such that the biosafety problem does not exist; jasmonate methyltransferase is derived from plants, and cannot cause biosafety problem after being expressed by Escherichia coli; the engineering bacteria are Escherichia coli, and has characteristics of simple culture conditions, low cost and simple implementation method; the method for improving the defense ability byincreasing the content of the hormone methyl jasmonate in the plant does not affect the growth of the plant; and with the application of the engineering bacteria in prevention and controlling of CornLump Smut, the significant prevention and control effect can be achieved, and the Escherichia coli strain reformed by gene engineering is worthy of being popularized and applied.

The disclosure describes jasmonate conjugates and nanocarried and / or microcarried jasmonate conjugates and pharmaceutical compositions thereof, as well as use thereof for treating or preventing angiogenesis-related or NF-κB-related disorders. Also disclosed are methods of making the conjugates.

The present invention relates to methods of treating benign hyperproliferative diseases of the epidermis by administering a composition comprising at least one jasmonate ester derivative, preferably methyl jasmonate. In particular, the present invention provides jasmonate ester derivatives as potent compounds useful for the treatment of disorders such as actinic keratoses with reduced side effects.

The invention belongs to a water-soluble fertilizer suitable for topdressing of apples in a coloring period and an application thereof. The fertilizer is prepared from the following raw materials: urea, industrial monoammonium, monopotassium phosphate, potassium nitrate, urea phosphate, phenylalanine, tyrosine, urine black acid, abscisic acid, gibberellin, jasmonate, ethylene, cane sugar, sodium nitroprusside and genistein. As a water-soluble topdressing fertilizer, the water-soluble fertilizer disclosed by the invention can realize topdressing in the coloring period of apples so as to realize rapid coloring of the apples, also considers the solubility of each component in the fertilizer, and can avoid the influence on the effect of the water-soluble fertilizer due to precipitation generated by interaction of the raw materials; the product has the advantages that after the bags outside the apples are picked off, leaf picking, fruit turning, flower thinning and film laying are not needed, the apples can be rapidly colored, and the fruits have both the appearance and the quality.

The invention provides a green planting method for edible rose, belonging to the field of plant cultivation. According to the invention, a pesticide with toxicity and harm to the human body is avoidedfrom being used as far as possible in the planting process of rose; meanwhile, the yield and nutritional value of the rose are improved; inorganic ammonium salt containing a coating layer is used ina culture medium used in the cutting process of the rose, and a green pest-resistant solution is used in the transplanting process of the rose, so weeds, diseases and pests are fundamentally reduced;and a jasmonate containing nutrient solution is used in the cultivation process of the rose, so the nutritional value of the rose is effectively enhanced. A single rose plant planted by using the method provided by the invention can reach a yield up to 1.64 kg, and can reach a soluble polysaccharide content up to 18.45%.

The invention belongs to the technical field of agriculture, and specifically relates to a cinnamon bark protecting agent and a preparation method. The cinnamon bark protecting agent is mainly prepared from the following raw materials in parts by weight: 5-9 parts of smomum villosum seed, 1-5 parts of citionella oil, 6-12 parts of long pepper, 3-8 parts of brassinolide, 6-9 parts of jasmonate, 6-12 parts of momordica cochinchinensis, 15-19 parts of geraniol, 20-25 parts of bornyl acetate, 5-10 parts of euphorbia thymifolia, 25-30 parts of tragacanth gum, and 4-6 parts of cleveland eucalyptus essential oil. The protecting agent, rich in active glucose, enzyme, nucleic acid, higher alcohol, unidentified growth factors, etc., can directly penetrate through surface cells of cinnamon to enter deep cells to take part in metabolism of crops, thus preventing the cinnamon bark from being attacked by germs and diseases and pests; and moreover, the output of the cinnamon can be effectively increased.