39 results about "Cold stress" patented technology

The invention discloses a low-carbon and energy-saving fattening pig house air conditioner control method which comprises the following steps: S1, constructing a grid digital twinborn model of a pig house, and initializing the grid digital twinborn model by using sensor data; s2, based on the gridding digital twin model, physical flow field simulation is executed, and temperature field distribution in the hog house is obtained; s3, temperature field distribution is analyzed, a temperature anomaly region needing to be regulated and controlled is recognized, and the attribute of the temperature anomaly region is quantified; s4, determining a target regulation and control unit and calculating regulation and control parameters of the target regulation and control unit based on the attribute of the temperature abnormal region; and S5, executing regulation and control and updating the digital twin model based on feedback data to form predictive closed-loop control based on physical simulation. By eliminating a local hot / cold stress area, the uniformity and stability of the hog house hot environment are greatly improved, meanwhile, a precise on-demand regulation and control mechanism is achieved, global excessive air supply is avoided, and substantive low-carbon and energy-saving effects in the operation process are achieved.

The invention discloses a pennisetum purpureum PpDREB1D-like gene and application thereof in improving cold resistance and biomass of plants, and relates to the technical field of plant genetic engineering. The PpDREB1D-like gene derived from the pennisetum purpureum is cloned and functionally identified for the first time, and experimental verification shows that the PpDREB1D-like gene can increase the cold resistance of plants, the growth state of the plants under cold stress is remarkably improved through overexpression of the PpDREB1D-like gene, the cold resistance of the plants is remarkably reduced through silence of the PpDREB1D-like gene, and the PpDREB1D-like gene can be used for improving the cold resistance of the plants. A molecular foundation is laid for popularization and planting of pasture and energy crops in cold regions, and the method has remarkable agricultural production and ecological value.

The application provides application of a calcium sensing protein ShCBL1 gene and belongs to the technical field of biology.The application overexpresses or superexpresses the ShCBL1 gene in sugarcane or rice, so that the content of proline and superoxide dismutase in the sugarcane or rice is increased, the content of malondialdehyde is reduced, and the cold resistance is improved.Researches show that when the ShCBL1 is superexpressed or overexpressed in plants, the cold stress related physiological indexes such as proline, superoxide dismutase (SOD) and malondialdehyde (MDA) can be significantly affected, so that the cold resistance of the rice and the sugarcane is enhanced.ShCBL1 as a positive regulator of cold resistance regulation can be used for molecular breeding of cold resistance varieties.

This invention provides a cold adaptation regulatory gene RAF3 / RAF6 This invention relates to the field of genetic engineering technology and its applications. Specifically, it provides a cold adaptation regulatory gene. RAF3 / RAF6 , wherein RAF3 The genome sequence is shown in SEQ ID No. 1. RAF6 The genome sequence is shown in SEQ ID No. 2. This invention also provides the... RAF3 / RAF6 The encoded protein, and its role in reducing RAF3 / RAF6 By reducing the expression level of a protein or decreasing its activity or content, the cold tolerance and photosynthetic efficiency of plants can be improved, meaning that under cold conditions, RAF3 and / or RAF6 It reduced photosynthesis while enhancing the expression of cold-response genes, ultimately inhibiting plant growth and promoting plant adaptation to cold stress.

The invention discloses a multi-stage heat recovery device for cultivation based on self-circulation air defrosting, which is characterized in that a first-stage heat exchange core and a second-stage heat exchange core are respectively arranged in a shell, and a first switching assembly and a second switching assembly are respectively arranged on the side surfaces of the first-stage heat exchange core and the second-stage heat exchange core in the shell; and each air inlet, each air outlet and the exhaust air outlet side of the second-stage heat exchange core are respectively provided with a temperature sensor. According to the device, a self-circulation air defrosting method is adopted, energy consumption is saved, operation is stable, meanwhile, the device is friendly to the animal house environment, excessive cooling capacity cannot be introduced, and the environment is greatly changed; meanwhile, the two switching assemblies are simple in structure and can be controlled by one motor; a double-core switching method in the device ensures continuous heat recovery, continuous work can be achieved in severe cold areas, sudden cooling of the environment of an animal house is avoided, and cold stress of livestock can be effectively prevented.

Compositions and methods for creating plants exhibiting enhanced resistance to abiotic stresses, especially cold stress are disclosed.

This utility model discloses a partial air intake ventilation system for the roof of a wide-body livestock shed, belonging to the field of livestock shed ventilation technology. It includes an air intake device at the front of the shed, a ventilation ceiling installed on the top of the shed, and an exhaust device at the rear of the shed. The roof area above the ventilation ceiling is connected to the air intake device, and the breeding area below the ventilation ceiling is connected to the exhaust device. Multiple ceiling air inlets connecting the roof area and the breeding area are installed on the ventilation ceiling. This ventilation system, by installing a ventilation ceiling on the top of the shed, allows cold air to first enter above the ventilation ceiling and then be evenly distributed to the breeding area from top to bottom through the ceiling air inlets. At this time, the heat inside the shed preheats the cold air above the ventilation ceiling, effectively improving the temperature uniformity and air freshness of the wide-body shed, avoiding cold stress caused by uneven airflow, and achieving precise air delivery to the breeding area, creating a more comfortable growing space for poultry and livestock.

The application discloses a kind of dairy cow cold stress multistage fuzzy comprehensive evaluation method, comprising the following steps: step (1): using analytic hierarchy process, from warm environment (temperature, relative humidity, wind speed and illumination), physiological factors (respiratory rate, body surface temperature) and air quality (NH3, CO2, PM 10 ) 3 dimensions construct dairy cow cold stress evaluation index system;Step (2): establish the judgment matrix of each index layer, and make consistency check, if meet the consistency requirement, then determine the weight of each index layer;Step (3): if it does not meet the consistency check, use genetic algorithm for testing and correcting the judgment matrix, the weight of each index layer is calculated by crossing, mutation and other operations;Step (4): the degree of cold stress of dairy cow is obtained by multistage fuzzy comprehensive evaluation;The application combines environmental parameters with dairy cow physiological parameters, and can realize comprehensive and accurate evaluation of dairy cow cold stress.

A cold stress evaluation method for livestock in cold and arid regions, characterized in that the evaluation method comprises the following steps: preprocessing related data, establishing an evaluation index system; constructing a cold stress fuzzy comprehensive evaluation model; applying the cold stress fuzzy comprehensive evaluation model, analyzing the cold stress comprehensive evaluation results, and obtaining accurate evaluation results of the cold stress of livestock; the specific cold stress conditions of the livestock group and different individuals in the livestock group can be accurately judged, thereby providing reasonable guidance and decision-making for the cold region farm in terms of warmth, ventilation, feed adjustment and medication.

The application discloses use of Bacteroides thetaiotaomicron in preparation of products for reducing cold stress response of ruminants. It is found that feeding Bacteroides thetaiotaomicron to ruminants, especially young calves, can effectively reduce the cold stress response of the ruminants.

The invention relates to an application of a corn ZmXTH27 gene in improving the cold resistance of plants. The ZmXTH27 gene encodes a protein of a xyloglucan endotransglycosidase / hydrolase (XTH) family, the gene expression level of the ZmXTH27 gene is remarkably increased under low-temperature stress, and a mutant zmxth27 of the gene shows a low-temperature sensitive phenotype. The ZmXTH27 gene is transformed into arabidopsis thaliana and is subjected to functional verification, and compared with wild type arabidopsis thaliana, the tolerance of an arabidopsis thaliana plant over-expressed with the ZmXTH27 gene to cold stress is remarkably enhanced. According to the invention, more theoretical bases are provided for corn cold resistance research, and gene resources and biotechnological means are provided for subsequent cultivation and germplasm resource innovation of new cold-resistant corn strains.

The utility model discloses a cold stress experiment temperature control box relates to temperature control box technical field, including main box body and the temperature control system of setting up on it, one side fixed connection of main box body has the additional position box main box body of experiment animal access main box body is equipped with the closed gas mechanism of opening and closing main box body and additional position box connecting position department and remaining low temperature gas in main box body, and the closed gas mechanism includes the side position disc of fixed connection on main box body, is equipped with multiple sets of triangle seal seat on side position disc, and is equipped with the ring seal subassembly of the drive multiple sets of triangle seal seat synchronous deflection to seal or open passageway groove on side position disc, the utility model discloses a closed gas mechanism is set up, when replacing test object, can effectively remain most low temperature air in main box body, and then greatly reduced the cold air loss of the test object replacement, thereby improves the experiment efficiency, reduced the operation cost of equipment, makes the cold stress experiment more energy -efficient.

The invention discloses a CAPS molecular marker developed based on a ZmMYB24 gene and related to salt resistance and low temperature resistance of corn and application of the CAPS molecular marker. Sequence variation of a ZmMYBR24 gene coding region induced and expressed by salt stress and low-temperature stress in different corn homozygous inbred lines is analyzed, correlation analysis is carried out on salt tolerance and low-temperature resistance of the corn homozygous inbred lines, correlation sites are mined, and then the CAPS molecular marker related to salt tolerance and low-temperature resistance of corn is developed and named as CAPs814. The molecular marker CAPs814 is used for marker-assisted selection, the average detection efficiency in identification of maize inbred lines with medium salt resistance and medium low temperature resistance or above is 80.56% and 86.11% respectively, and the molecular marker CAPs814 provides a new technical means for screening maize materials with medium salt resistance and low temperature resistance.

This invention discloses a type of alfalfa MsCYP Genes and their applications in salt and cold tolerance. This invention, based on phenotypic and genome association analysis, screened a gene in alfalfa that responds to salt and cold stress. MsCYP The MsCYP The nucleotide sequence is shown in SEQ ID No. 1, and the amino acid sequence is shown in SEQ ID No. 2. Further overexpression in alfalfa hairy roots... MsCYP The results showed that under salt stress or a low temperature of 4°C, overexpression MsCYP The hairy roots of the gene are longer than those of the wild type, and the plant exhibits greater tolerance to salt and low-temperature stress than the wild type, indicating that the gene is overexpressed. MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsC Genes can enhance the salt and cold tolerance of alfalfa. This invention provides theoretical and practical support for the breeding and widespread promotion of salt- and cold-tolerant alfalfa varieties.

The invention discloses a novel thermal bridge structure for a vacuum heat-insulation cryogenic pressure vessel, the thermal bridge structure is a two-section shell thermal bridge and is formed by assembling and welding two end sockets, one end socket is large in opening size and is not in contact with a liquid phase pipeline, and the other end socket is used for fixing the liquid phase pipeline; meanwhile, a feedback heating system is configured and comprises a temperature sensor, a controller and a heating element, and a PID control algorithm and a temperature prediction algorithm are arranged in the controller. Through the two-section structural design, the heat exchange area is increased, external heat preservation is facilitated, precise temperature control is achieved in combination with a feedback heating system, and the heating power is dynamically adjusted through a PID algorithm according to the temperature deviation; the temperature prediction algorithm can pre-judge the temperature trend 5-10 minutes in advance and regulate and control the temperature trend in advance. The structure effectively improves the adaptability in an extremely low temperature environment, ensures that the temperature of the root of the thermal bridge is stabilized in a carbon steel application range, prolongs the service life of the pressure vessel, and is suitable for liquid phase pipeline fixing and heat preservation scenes of a fixed vacuum heat insulation cryogenic pressure vessel.

This invention discloses a StGAD1 The application of genes in regulating low-temperature resistance in potatoes belongs to the field of biotechnology. This invention is the first to obtain potato glutamate decarboxylase in potatoes. StGAD1 Genes, and through the use of Agrobacterium-mediated methods StGAD1 Gene knockout in potatoes was used for functional verification, and the results were obtained. StGAD1 Gene deletion makes potato plants more sensitive to cold stress, reflecting StGAD1 Genes play an important role in responding to low temperature stress, opening up new avenues for crop stress resistance breeding.

The present application relates to the technical field of traditional Chinese medicine processing, and particularly relates to a processing method for regulating the quality of Yunnan rhizoma polygonati by stress, which uses fresh Yunnan rhizoma polygonati as raw material, and adopts a programmed temperature control method to perform stress, washes, processes, slices and dries the stressed Yunnan rhizoma polygonati to obtain a finished product; the stress is heat stress or cold stress. Compared with the prior art, the present application gradually changes the stress of the collected fresh Yunnan rhizoma polygonati, avoids the damage to the physiological structure of the plant caused by the rapid change or large amplitude of the temperature, and thus affects the ability and efficiency of the plant to secrete secondary metabolites. The present application significantly increases the polysaccharide content of Yunnan rhizoma polygonati from the source production site processing link, improves the activity of sucrose synthase, and improves the quality of Yunnan rhizoma polygonati. The present application is simple and easy to operate, and provides a new idea for the new production site processing method of medicinal Yunnan rhizoma polygonati.

The invention belongs to the technical field of biology, and particularly relates to a method for identifying cold resistance of wheat in a seedling stage by utilizing autophagy protein ATG8. The method comprises the following steps: constructing a vector of EGFP (enhanced green fluorescent protein)-ATG8, carrying out transient expression on leaves of different wheat varieties, and observing subcellular localization change of the EGFP-ATG8 when the EGFP-ATG8 is subjected to cold stress, so as to identify the cold resistance of the wheat; compared with an existing scheme, subcellular localization of EGFP-ATG8 can be observed only under the low-temperature stress condition of 4 DEG C, the cold resistance of EGFP-ATG8 can be known, and the method has the advantages of being low in cost, easy to operate and high in precision.

The invention discloses a perinatal dairy cow cold stress degree evaluation method, device and equipment and a medium, and relates to the field of perinatal dairy cow cold stress degree evaluation.The method comprises the steps that intelligent necklace data, visible light video data and infrared video data of a perinatal dairy cow are obtained; performing feature extraction on the intelligent necklace data to obtain a first ingestion feature, performing feature extraction on the visible light video data to obtain a second ingestion feature, and performing feature extraction on the infrared video data to obtain a feed pile shape feature; taking the first ingestion feature, the second ingestion feature and the feed pile shape feature as input, and utilizing a trained dairy cow cold stress identification model to predict a target cold stress evaluation result of the perinatal dairy cow; the target cold stress assessment result of the perinatal dairy cow is displayed, the cold stress degree of the perinatal dairy cow can be assessed more timely, comprehensively and accurately, real-time performance is achieved, and early warning can be carried out in advance.

The invention discloses a liriodendron hybrids LhARIA gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the liriodendron hybrids LhARIA gene provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the expressed protein is as shown in SEQ ID NO.2. The expression mode of the LhARIA gene under cold, drought and salt stress is analyzed through a q-PCR technology, and it is found that the gene is remarkably up-regulated under the cold stress condition. An overexpression experiment of the LhARIA gene is respectively carried out in arabidopsis thaliana and hybrid liriodendron, and a result shows that the LhARIA gene remarkably improves the cold resistance of a transgenic plant through multiple mechanisms of regulating ROS metabolism, enhancing the antioxidant enzyme activity, activating an ICE-CBF-COR signal channel and the like. The invention provides important candidate genes and theoretical support for improving the cold resistance of plants by using a genetic engineering technology in future.

The invention relates to the field of rice gene engineering, discloses application of a rice gene OsNFX1 in regulation and control of cold resistance, and particularly relates to application of an OsNFX1 gene knockout mutant in the aspect of cold resistance. A CRISPR-Cas9 gene editing technology is utilized to edit a rice gene OsNFX1, and the expression of the gene is knocked out, so that the cold-resistant rice mutant is obtained. Experimental results show that compared with wild type rice, the survival rate of seedlings of the osnfx1-1 and osnfx1-2 mutants under the cold stress condition is remarkably improved. The research provides a new strategy for improving the adaptability of the rice in the cold environment, and provides a theoretical basis and practical guidance for agricultural production.

The application discloses a piglet delivery powder with somatic heat generation efficacy, which comprises modified porous starch, high-thermal-conductivity mineral filler, hygroscopic and exothermic salt, skin-acceptable organic acid, plant-derived antibacterial component and flow aid agent. The capillary effect of the modified porous starch can quickly adsorb body surface moisture within 0.1 seconds, synchronously trigger the instantaneous hydration reaction of the hygroscopic and exothermic salt, and uniformly conduct heat to the whole body by means of the heat conduction network formed by the high-thermal-conductivity mineral filler, so that active, rapid and uniform somatic heat generation is realized. The piglet delivery powder can provide somatic warmth within 0.1 seconds after the birth of a newborn piglet, effectively resist cold stress, greatly shorten the body surface drying time to 8.5 seconds, promote timely intake of colostrum, greatly improve the survival rate, and avoid the stimulation of local overheating on the skin, and has outstanding practical value.

The application discloses a PgEGY3 gene for improving cold resistance and increasing leaf fresh weight of Pennisetum, and application thereof, relates to the technical field of genetic engineering, and the nucleotide sequence of the PgEGY3 gene of the Pennisetum is shown in SEQ ID NO. 1. The application also discloses a protein coded by the PgEGY3 gene of the Pennisetum. The application also discloses a recombinant expression vector and a recombinant host cell comprising the PgEGY3 gene of the Pennisetum. The application also discloses application of the PgEGY3 gene, the recombinant expression vector and the recombinant host cell in improving cold resistance and biomass of the Pennisetum. The application verifies that the PgEGY3 gene of the Pennisetum can increase the cold resistance of plants, and overexpression of the PgEGY3 gene significantly improves the growth state of plants under cold stress.

The invention discloses a method for improving traits of hippeastrum hippeastrum, which is characterized in that CYCD1 gene in hippeastrum hippeastrum is highly expressed by an agrobacterium-mediated method, so that the anti-freezing capability of hippeastrum hippeastrum plants can be improved; the relative conductivity of hippeastrum hippeastrum plants under the freezing stress condition is reduced; the content of malondialdehyde in hippeastrum hippeastrum plants under the freezing stress condition is reduced; the content of super-superoxide dismutase in hippeastrum hippeastrum plants under the freezing stress condition is increased; the content of peroxidase in hippeastrum hippeastrum plants under the freezing stress condition is improved. The method has an application prospect.

This invention provides the application of cytokinin-responsive factors in improving the resistance of potatoes to low-temperature stress, relating to the field of biotechnology. This invention is the first to identify the CRF family in potatoes. Through collinearity analysis and conserved domain screening, nine CRF family members were successfully identified from the potato genome. Functional studies of the CRF family members in potatoes revealed that StCRF3a plays a functional role in low-temperature stress in potatoes. Specifically, StCRF3a is induced by low temperature, and its promoter region contains a low-temperature responsive element.

The invention provides a broiler chicken raising method based on nutrition regulation and environmental stress induction, and belongs to the technical field of livestock breeding, and the broiler chicken raising method comprises the following steps: dividing a raising cycle into four stages of 1-14 days old, 15-28 days old, 29-35 days old and 36-45 days old according to time, and performing nutrition regulation and environmental induction on the four stages, feed containing flaxseed meal and fish oil is fed at the 29-35-day-old stage, choline and betaine are added, periodic mild cold stress treatment is combined, and unsaturated fatty acid is promoted to be deposited into muscles; 80% limited feeding is carried out at the 36-45-day-old stage, meanwhile, a yeast extract, L-glutamine and a flavor enhancer composed of betaine and allicin are supplemented, the content of flavor substances such as inosinic acid and glutamic acid is effectively increased, and the flavor of the broiler chickens is remarkably improved. Meanwhile, muscle fibers are refined and oxidative damage is relieved by reducing the feeding density, adjusting illumination, prolonging the free-ranging time and the like.

The poultry house ventilation structure comprises a house body, an outer wall and a suspended ceiling, the house body comprises a side wall and a roof, the outer wall is arranged on the outer side of the house body and spaced from the side wall, a first heat exchange area is formed between the outer wall and the side wall, a first air opening is formed in the lower end of the outer wall, a second air opening is formed in the upper end of the side wall, and the suspended ceiling is connected into the house body. A second heat exchange area is formed between the suspended ceiling and the roof, the second air opening is communicated with the second heat exchange area, and the suspended ceiling is provided with an air exchange opening communicated with the feeding area in the house body. A double-layer wall structure is formed by the side wall and the outer wall, the first heat exchange area in the double-layer wall absorbs heat in the house and stores the heat, and hot air exchanges heat with cold air entering the first heat exchange area outside the house, so that the temperature of the cold air is increased; by means of the double-ceiling structure, rising hot air in the house is collected and exchanges heat with cold air, the air heat is further improved, and therefore the probability that livestock produce raw and cold stress can be greatly reduced, and the breeding quality of the livestock is improved.

The invention provides a method for analyzing jujube ZjGGP gene response cold stress to promote ascorbic acid accumulation, and relates to the technical field of plant molecular biology, and the method comprises the following five steps: sample preparation and treatment, ascorbic acid content determination, gene expression profile analysis and key gene screening, key gene expression verification and causal relationship determination. According to the method disclosed by the invention, short-term cold treatment is an optimization process window for inducing the ascorbic acid of the jujube fruits to be accumulated to a peak value, accurate temperature and time parameters are provided for postharvest cold-chain logistics, and the core nutritional value of the jujube fruits can be actively maintained and improved through a physical regulation and control means on the premise of not depending on a chemical preservative; the shelf life and the commodity quality are obviously prolonged. Meanwhile, the determination of the ZjGGP gene provides valuable molecular targets and selection markers for molecular breeding of jujube trees.

The application relates to a corn ZmXTH27 gene in improving the cold tolerance of plants. The ZmXTH27 gene encodes a xyloglucan endotransglycosylase / hydrolase (XTH) family protein, the expression level of the gene is significantly up-regulated under low temperature stress, and a mutant of the gene zmxth27 shows a low temperature sensitive phenotype. The ZmXTH27 gene is transformed into Arabidopsis thaliana and functionally verified, compared with wild type Arabidopsis thaliana, the cold stress tolerance of the Arabidopsis thaliana plant overexpressing the ZmXTH27 gene is significantly enhanced. The application provides more theoretical basis for the cold resistance research of corn, and provides gene resources and biological technology means for the cultivation of new cold-resistant corn strains and the innovation of germplasm resources.