154 results about "Cold stress" patented technology

The invention discloses anti-stress feed additives. The compositions of the anti-stress feed additives are: 15 to 30 percent of astragalus polysaccharides, 15 to 30 percent of radix scutellariae glucoside, 10 to 20 percent of pennyroyal wrappage, 10 to 20 percent of honeysuckle extract, 0.5 to 5 percent of vitamin C, 0.5 to 5 percent of vitamin E, 0.001 to 0.02 percent of chromium picolinate, 0.05 to 0.3 percent of zinc oxide, 0.1 to 1 percent of arginine, 1 to 5 percent of tryptophan, 1 to 5 percent of glutamine, 1 to 5 percent of sodium sulfate, 1 to 3 percent of calcium iodate, 1 to 3 percent of sodium selenite, 1 to 3 percent of potassium dihydrogen phosphate, the balance being auxiliary materials. The anti-stress feed additives are added into poultry feed according to the ratio of 0.1 to 0.3 percent. The anti-stress feed additives have the advantages that: firstly, the anti-stress feed additives can reinforce the anti-stress capability of poultry, and are particularly suitable for reinforcing the cold stress capability and the heat stress capability; secondly, the anti-stress feed additives can quickly replenish electrolyte loss caused by dehydration, and quickly recover the osmotic pressure of cells and adjust the acid-base balance in the body; and thirdly, the anti-stress feed additives can reinforce the anti-positioning capability of water bodies of the aquatic animals.

Apparatus and methods are provided for introducing an alkane to a plant in order to stimulate the plant's growth. Any type of plant may be treated, including potted plants, shrubs, trees, lawns, agricultural crops and the like. In a preferred embodiment, the alkane comprises butane, but other compounds can be used, including methane, ethane and propane. The alkane may be combined with water or another liquid carrier, or plant growth-enhancing additives such as nutrients, insecticides and alkane-utilizing bacteria. To induce aerobic conditions, oxygen-containing gas such as air may be introduced along with the alkane or separately from the alkane. The introduction of alkane may enhance plant growth by increasing the indigenous microbial populations in soil, e.g. bacteria, fungi, protists, and prokaryotes, which may provide benefits such as increased nutrient uptake, faster root development, and reduced heat, drought and cold stress, resulting in enhanced plant growth.

The invention discloses a probiotic foodstuff and a preparation method thereof. In the fermentation process of yoghourt, a functional starter, germinated brown rice, Chinese herbal medicine extract with anti-heat-and-cold irritability and a stabilizing agent capable of effectively enhancing stability are added; temperature-varying yoghurt fermentation enables propagation of lactic acid bacteria to be maximized and anti-heat-and-cold stress capability of lactic acid bacteria to be improved; non-thermal processing technology is employed for pretreatment of yoghurt and fruit and vegetable pulp so as to prevent infection with infectious microbes in preparation process, improve juice yield of the fruit and vegetable pulp, increase the content of soluble cellulose in the fruit and vegetable pulp and prevent browning of the fruit and vegetable pulp; and a scientifically compounded cryoprotectant with good anti-refrigeration effect is added during mixing of the fruit and vegetable pulp and the yoghurt. Eventually, the prepared probiotic foodstuff has fine mouthfeel, uniform texture, stable state, natural quality, a great number of live bacteria, strong functionality and long shelf life, wherein the content of the live bacteria is 1.75 * 10<11> to 2.55 * 10<11> CFU/g, and the shelf life is 30 to 36 months at normal temperature.

The invention relates to the technical field of agriculture, and discloses a paddy biofertilizer, a preparation method thereof and bacillus subtilis. The preparation method comprises the following steps of: adding bacillus subtilis with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6190 into a solid material, fermenting until the quantity of bacillus subtilis is at least 1.2*10<10>/g, the spore percentage reaches at least 60% and the impurity microorganism percentage is less than 20%, and then uniformly mixing the fermented solid material with humic acid, agricultural rare earth, aged and fermented poultry and livestock feces, silicon-calcium-magnesium fertilizer and amino acid fertilizer so as to obtain the paddy biofertilizer. According to the invention, the mutagenic bacillus subtilis is mixed with chicken manure and other aged and fermented organic matters, humic acid, silicon-calcium-magnesium fertilizer, agricultural rare earth, amino acid fertilizer and the other substances to form the compound paddy microbe manure, so that the number of tillers of the paddy is increased, the tillering stage of the paddy is advanced, the root weight of the paddy is increased, the leaf color of the paddy is enhanced, the water temperature is raised, and the growth of the paddy under the cold stress is promoted.

The present invention relates to the mitogen-activated protein kinase called MAPK5. The rice MAPK5 gene, its protein and kinase activity were induced by abscisic acid, pathogen infection, wounding, drought, salt and cold temperature. However, suppression of MAPK5 expression and kinase activity in dsRNAi transgenic plants resulted in constitutive expression of pathogenesis-related genes such as PR-1 and PR-10 but enhanced resistance to fungal and bacterial pathogens. In contrast, overexpressed transgenic lines exhibited elevated MAPK5 kinase activity and increased tolerance to drought, salt and cold stresses. This invention provides methods for increasing tolerance to abiotic and biotic stress in plant using MAPK5.

The invention discloses a method for increasing the weight gain of a weaned piglet. The method for increasing the weight gain of the weaned piglet comprises the step of comprehensively carrying out a multistage feeding method, a two-stage heat-insulating method and a dry and wet two-trough method to the weaned piglet. The method can reduce the cold stress of the weaned piglet and lower the diarrhea rate, reduces intestinal villi atrophy and the weakling piglet rate, improves the survival rate of piglets, and remarkably increases the weight gain of the weaned piglet.

The present invention relates to a thermoplastic resin composition. In more particular, a thermoplastic resin of graft ABS polymer and heat-resistant copolymer contains acryl-based resin, peroxide-based additive, or cross-linking additive, so that the thermoplastic resin composition of the present invention is effective in processing of a colored heat resistant ABS resin by having excellent cold stress whitening reduction and strength property, without lowering other properties as well as giving excellence in hot-tool weldability, pigmentation and paint spread property which are more important in post process, and maintaining the mechanical property as well as giving excellent heat-resistance and low gloss property.

The invention discloses a freeze-dried foodstuff and a preparation method thereof. In the fermentation process of yoghourt, a functional starter, germinated brown rice, Chinese herbal medicine extract with anti-heat-and-cold irritability and a stabilizing agent capable of effectively enhancing stability are added; temperature-varying yoghurt fermentation enables propagation of lactic acid bacteria to be maximized and anti-heat-and-cold stress capability of lactic acid bacteria to be improved; non-thermal processing technology is employed for pretreatment of yoghurt and fruit paste so as to prevent infection with infectious microbes in preparation process, improve fruit juice yield, increase the content of soluble cellulose in the fruit paste and prevent browning of the fruit paste; and a scientifically compounded cryoprotectant with good anti-refrigeration effect is added during mixing of the fruit paste and the yoghurt. Eventually, the prepared freeze-dried foodstuff has fine mouthfeel, uniform texture, stable state, a great number of live bacteria, strong functionality and long shelf life, wherein the content of the live bacteria is 1.55 * 10<11> to 2.52 * 10<11> CFU/g, and the shelf life is 30 to 36 months at normal temperature.

The invention discloses a high cold and heat stress indoor climate simulation device. The high cold and heat stress indoor climate simulation device comprises a climate chamber, an indoor air conditioning system, an air interlayer, an interlayer climate regulating system, a climate chamber wall surface radiation system and a monitoring and controlling system; a side wall of the climate chamber is provided with a thermal insulation sealed door; the air interlayer is defined by the side wall, a chamber roof and a floor of the climate chamber and an outer wall, a roof and a floor, wherein the outer wall, the roof and the floor are arranged on the outer side of the climate chamber corresponding to the side wall, the chamber roof and the floor of the climate chamber respectively. The high cold and heat stress indoor climate simulation device comprises the indoor air conditioning system, the interlayer climate regulating system, the climate chamber wall surface radiation system and the monitoring and controlling system and accordingly the high temperature, high humidity and high heat stress environmental simulation and the low temperature and high cold stress environmental simulation can be simultaneously implemented and the environmental thermal simulation can also be implemented. Regulation and control can also be performed on the indoor climate through the indoor air conditioning system to simulate the extreme working conditions, environment parameters are stable and controllable, and accordingly true reliable research results can be ensured.

The invention relates to a method for using lanthanum to improve cold resistance of tall fescue turf based on garbage compost medium, which includes: adding 70g of compost into each petri dish, sowing 1.5g of weighed seeds of tall fescue into each petri dish, and allowing the seeds to germinate and grow preliminarily at the room temperature; controlling the indoor temperature to be 18-28 DEG C and the relative humidity to be 40%-60% during the period of germination and preliminary growth, adding 0.2-0.4g, kg-1 lanthanum nitrate into the compost medium 16 days after sowing; continuing cultivation for 6 days after the lanthanum nitrate is added, moving the petri dishes into a lighting culture box, subjecting the petri dishes to cold stress treatment, and testing adversity resistance indexes when cold stress treatment is performed for 3-9 days, wherein cold stress temperature is set as 15/10 DEG C at night, lighting time is set as 9h, light intensity is set as 100 and the testing cycle is set as 34 days. Test results show that tall fescue can be induced to resist cold stress by lanthanum treatment and damage of the tall fescue by cold can be alleviated.

The invention relates to a method for separating and purifying plant ice structural protein, which belongs to the field of food additive separation. The method uses cold-stress plants as raw materials to separate and purify the ice structural protein by leaching and adopting a cloud point extraction method, and comprises the following steps: firstly, adopting buffer solution containing a non-ionic surfactant to leach out the ice structural protein from the plant raw materials; secondly, performing cloud point extraction by adopting a micellar solution formed by the non-ionic surfactant, wherein the mixture is divided into a water phase and a micellar phase after being heated to the cloud point temperature, hydrophobic impurities are enriched at the micellar phase, while the hydrophilic ice structural protein is kept at the water phase; and finally, performing dialysis concentration and freeze drying to obtain a white ice structural protein mixture. A differential scanning calorimetry is adopted to perform thermal hysteresis activity detection on a separated and purified sample to prove that the product has high thermal hysteresis activity. The method has the advantages of simple separation process, low production cost, no volatile organic solvent in the process of extraction and high purification efficiency, and is advantageous for producing the ice structural protein industrially and making full use of plant resources.

The invention belongs to the fields of animal nutrition and feed science, and relates to ration preparation method for fattening beef cattle resistant to the low temperature and moisture climatic conditions. The technology solves the problems of reduction of daily gain and increase of disease incidence in fattening the beef cattle under the low temperature and moisture climatic conditions. The ration comprises corn, soybean meal, capsicum residue, fruit residue, cottonseed meal, bran, sunflower meal, rapeseed meal, calcium hydrophosphate, rock flour, premix, sodium bicarbonate and the like (based on the weight percentage of dry matter); and the roughage comprises wild hay, corn stalk, silage and the like. Besides, the premix is added with riboflavin (vitamin B2) and added with more vitamin A and vitamin E. The ration is added with the capsicum residue which contains capsaicin and flavonoids, the fruit residue which is rich in vitamin C, and the riboflavin (vitamin B2) with anti-cold stress, and is added with more vitamin A and vitamin E, so that the ration applied under the low temperature and moisture climatic conditions can increase the feed intake, the daily gain and the feed conversion of the beef cattle, the lean percentage of the beef, the palatability of the feed, the digestibility of the roughage and the economic benefit of feeding, and reduce the disease incidence.

The invention provides a low-temperature preservation culture medium of rubber tree embryonic callus and a low-temperature method of the culture medium, belonging to the technical field of tissue culture. Exogenous antioxidant L-glutamine, oxidation-resistant vitamin VC and exogenous hormone salicylic acid are added in the low-temperature preservation culture medium; endogenous antioxidant enzyme glutathione reductase GR action is motivated to maintain intracellular high-proportion GSH/GSSG and SG so as to participate in ascorbic acid glutathione circulation AsA-GSH of a key approach for eliminating ROS in a plant, other antioxidant enzyme is supplemented to synergistically and effectively remove residual ROS in the embryonic callus preserved at low temperature so as to improve the cold stress resistance of the embryonic callus. The callus survival rate of the embryonic callus preserved by a low-temperature preservation culture medium is not significantly different from that of the embryonic callus cultured in a dark condition at 25 plus/minus 2 DEG C, and the FDA fluorescence detection indicates that the cell viability of the embryonic callus subjected to low-temperature preservation is maintained stronger.

The invention discloses an enhanced winding pipe manufacturing method and an enhanced winding pipe manufactured through the enhanced winding pipe manufacturing method. According to the method, firstly, an enhancing belt is spirally wound around the outer surface of a structural wall pipe to from an enhancing layer, and then wrapping is conducted on the outer surface of the enhancing layer to form a sheet layer connected through hot melting. The method has the beneficial effects that through the hot melting manner that overlapping and wrapping are conducted on the adjacent two sides of a sheet, cold stress can be avoided effectively, and potential layering hazards are avoided. The invention further discloses an enhanced winding pipe manufacturing device. The enhanced winding pipe manufacturing device comprises a machine base, a fixing device capable of fixing the structural wall pipe, a drive device capable of driving the winding pipe to rotate and driving the structural wall pipe to move in a straight and reciprocating manner along a movable rack, a belt unwinding device through which the enhancing layer is wound around the outer surface of the structural wall pipe and a sheet wrapping device, wherein the sheet wrapping device is used for achieving the operation that the sheet is wound around the outer surface of the enhancing layer in an overlapping and wrapping manner so as to form the sheet layer connected through hot melting. The enhanced winding pipe manufacturing device has the beneficial effects of being simple in structure, convenient to operate, safe and reliable.

The invention discloses application of cotton gene GhDTX27 in plant salt, drought and cold stress tolerance. Gene ID of the cotton gene GhDT27 is Gh_D06G0281. A transgenic GhDTX27 Arabidopsis thalianahomozygous line acquired by a transgene technology has significantly improved tolerance to salt, drought and cold stress; the various verifications are carried out, such as phenotypic change measurement after salt, drought and cold treatment, chlorophyll content measurement, measurement of relative water content of leaves, ionic conductivity measurement, water loss measurement for detached leaves, germination rate counting, root elongation comparison, seedling fresh weight changes, expression analysis for adversity stress response genes, antioxidase activity measurement, and oxidant content measurement. The above results indicate that GhDTX27 plays an important role in plant salt, drought and cold stress tolerance.

Visible light is a treatment option for Segmental Vitiligo (SV), and visible light-induced repigmentation is associated normalization of sympathetic. Currently it is difficult to predict individual patient's response to visible light therapy. Therefore, the present invention uses the Laser Doppler Flowmeter to serve as a response predictor for visible light on treating SV. The present invention recruited 14 Segmental Vitiligo patients for evaluating clinical information. FirstLaser Doppler Flowmeter was used to evaluate the cutaneous blood flow of SV lesion and contralateral normal skin, and then treated them with visible light irradiate, cold-stress, rewarmed, and recorded the change of skin blood flow, finally the patients received regular visible light treatment for 3 months, and patients have a sign of repigmentation after the treatment.

The invention discloses a feed additive capable of improving beef cattle cold resistance and relates to the filed of feed additives. The feed additive capable of improving beef cattle cold resistance solves the problems that under a cold stress condition, a feed conversion rate, a weight gain rate and immunity of beef cattle are reduced and endocrine disorder of beef cattle is caused. The feed additive capable of improving beef cattle cold resistance comprises Chinese angelica, root of pilose asiabell, radix glycyrrhizae preparata, baical skullcap root, safflower, motherwort, argy wormwood leaf, medicated leaven, rhizoma atractylodis, cassia bark, dried ginger, rose root and monkshood. The feed additive capable of improving beef cattle cold resistance can be added into beef cattle concentrated feed according to a weight percentage of 2% and after uniform stirring, the beef cattle concentrated feed with the feed additive can be directly used. Through the utilization of the feed additive capable of improving beef cattle cold resistance, in a cold season, it is obviously relieved that a beef cattle feed conversion rate and a beef cattle weight gain rate are reduced because of cold stress; human immunity is improved; and normal endocrine is maintained. The feed additive capable of improving beef cattle cold resistance is prepared from cheap and easily acquired Chinese herbal medicines having wide sources, does not produce drug resistance, does not remain in beef products, allows a low utilization amount and has a low cost.

The invention discloses a fruit and vegetable probiotic tablet and a preparation method thereof. In the fermentation process of yoghourt, a functional starter, germinated brown rice, Chinese herbal medicine extract with anti-heat-and-cold irritability and a stabilizing agent capable of effectively enhancing stability are added; temperature-varying yoghurt fermentation enables propagation of lactic acid bacteria to be maximized and anti-heat-and-cold stress capability of lactic acid bacteria to be improved; non-thermal processing technology is employed for pretreatment of yoghurt and fruit and vegetable pulp so as to prevent infection with infectious microbes in preparation process, improve juice yield of the fruit and vegetable pulp, increase the content of soluble cellulose in the fruit and vegetable pulp and prevent browning of the fruit and vegetable pulp; and a scientifically compounded cryoprotectant with good anti-refrigeration effect is added during mixing of the fruit and vegetable pulp and the yoghurt. Eventually, the prepared fruit and vegetable probiotic tablet has fine mouthfeel, uniform texture, stable state, natural quality, a great number of live bacteria, strong functionality and long shelf life, wherein the content of the live bacteria is 1.75 * 10<11> to 2.55 * 10<11> CFU/g, and the shelf life is 30 to 36 months at normal temperature.

The invention discloses the application of a straw mushroom manganese superoxide dismutase VMn-SOD in improving the stress resistance ability of microorganisms. According to the straw mushroom manganese superoxide dismutase VMn-SOD, an amino acid sequence is shown as SEQ ID NO.1, and a nucleotide sequence of a coding gene is shown as SEQ ID NO.2. The straw mushroom manganese superoxide dismutase VMn-SOD gene is transferred into a suitable microbial host body, so that a host expresses the manganese superoxide dismutase, the heat stress resistance, cold stress resistance and salt stress resistance ability of the host are improved. Straw mushroom catalase VCAT is derived from fungi and transferred into the suitable microbial host, and the temperature stress resistance ability and the salt stress resistance ability of the host are improved.

The invention discloses a raising method for increasing the egg laying rate of spotted-brown chickens in winter. The basic metabolism level of the spotted-brown chickens can be improved, the cold stress of the spotted-brown chickens in winter is reduced, the egg laying amount of the spotted-brown chickens in winter is increased, and the requirements of people for eggs in daily life are met. The spotted-brown chickens are subjected to scientific and standardized raising management, harmful influences of various adverse situations on the spotted-brown chickens can be reduced to the maximum extent, an environment most beneficial for health and egg laying is provided for the spotted-brown chickens, and the genetic potential of the spotted-brown chickens can be fully exerted; the raising method is adopted for raising, the egg laying rate of the spotted-brown chickens is high, and nutrition of the eggs is rich.

The invention relates to a special feed additive for meat-purpose quail for resisting cold stress and improving meat quality, and the special feed additive is used for the general problems of frequent enteritis and poor meat quality of quail in northeast China caused by cold stress. Various ingredients carry out synergistic interaction to play the functions of resisting cold stress by procyanidolic ologomer and improving the meat quality, which are attempts and breakthroughs in the feed field. The additive is low in use amount, low in cost, environment-friendly, non-toxic and suitable for large-scale popularization and application.

The invention provides a method for preparing germinated brown rice by the repeated cold stress forcing method. The method comprises the following steps: placing the brown rice in low temperature solution to force cell tissues of the brown rice to accumulate reactive oxygen; and promoting content of soluble saccharide, free amino acid and polyphenol material to increase. In addition, to prevent excessive reactive oxygen from doing harm to cells, the repeated and short-time low temperature forcing and high temperature stratification are alternatively employed to cause the reactive oxygen to begenerated slowly; as a result, not only high soluble saccharide, free amino acid and polyphenol material are obtained, but also germination percentage of the brown rice is improved; and finally, lower temperature is employed to force the cell membrane of the brown rice to be damaged, so that the soluble sugar and the free amino are extravasated and the new product of the germinated brown rice with good mouthfeel can be produced.

The invention discloses an additive for resisting coldness and accelerating egg laying for laying ducks and application thereof, relating to an additive for accelerating egg laying and application thereof. The additive solves the problems that the laying ducks of northern China have lowered physique, increased feed and decreased egg laying quantity in cold seasons with low temperature. The additive for resisting coldness and accelerating egg laying for the laying ducks is composed of Vitamin E, Vitamin C, Vitamin B2, selenium and rest of carrier. The application of the additive is that under the condition of cold stress to the laying ducks, 0.035 to 0.045% of additive for resisting coldness and accelerating egg laying for the laying ducks is added in adequate diet for the laying ducks. The invention improves nonspecific resistance of the laying ducks as well as anti-stress capability, prevents physique from declining, and lightens damage caused by cold stress, so that the laying ducks are in favorable physiological state and perform maximal productivity, feed consumption is normal, eggs laid by the laying ducks have normal size and weight, and egg laying rate is increased from 10% to 16%; the additive has the advantages of small addition and low cost, so that the additive is suitable for popularizing and utilizing on large scale.

The invention relates to a promoter for the specificity expression and the later development expression of a plant tissue and application thereof, belonging to the biotechnology field. In the invention, a promoter sequence of a cotton cyclopropane fatty acid synthase with the length of 2.6kb is obtained. Place analysis proves that the promoter sequence contains a CAAT box, a TATA box, a cupric ion induced expression element, a pathogenicbacteria induced expression element, a dry response element, a cold stress response element, and the like as well as some root specificity expression elements and floral organ specificity elements. After GUS (beta-Glucuronidase) is dyed, a traditional gene is specially expressed at a later development stage by the promoter, and the expression parts are only restricted at a stem base part, a root and an anther.

The invention provides a monitoring and early warning device and method for human multiple physiological parameters under extreme high and low temperature environments. The device comprises a multiplephysiological parameter acquisition unit, a control unit, an early warning unit, a display unit, a storage unit, a communication unit and a power supply unit; the multiple physiological parameter acquisition unit is connected to the control unit through the communication unit; and the control unit is connected to the early warning unit, the display unit and the storage unit through the communication unit. The method monitors a plurality of human physiological parameters including an electrocardiogram parameter, a blood oxygen parameter, a blood pressure parameter and multi-point skin temperatures, calculates an average skin temperature, metabolic rate, core temperature, cold stress index or a thermal stress index value of the human body, and performs grading early warnings for the risks of personnel, thereby greatly improving the safety of emergency responders when working in extremely high and low temperature environments.

The invention discloses application of hormones to cold resistance and disaster mitigation of booting stage wheat. The hormones are preferably progesterone. During application, the progesterone at 0.05 mu mol/L by concentration is sprayed on leaves 24 hours after crops of the booting stage suffer from chilling damage. According to the application of the hormones to cold resistance and disaster mitigation of the booting stage wheat, by spraying the progesterone onto the crops of the booting stage which suffer from chilling damage, cold resistance of the booting stage wheat can be effectively improved, and influence of cold stress on growth and the yield of the wheat during the booting stage can be relieved.