75 results about "Cold stress" patented technology

The invention discloses a PgEGY3 gene for improving cold resistance of pennisetum alopecuroides and increasing fresh weight of leaves and application of the PgEGY3 gene, and relates to the technical field of genetic engineering, and the nucleotide sequence of the PgEGY3 gene of the pennisetum alopecuroides is shown as SEQ ID NO.1. The invention also discloses a protein coded by the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses a recombinant expression vector and a recombinant host cell comprising the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses application of the PgEGY3 gene, a recombinant expression vector and a recombinant host cell in improving cold resistance and biomass of pennisetum alopecuroides. It is verified that the Pennisetum alopecuroides PgEGY3 gene can improve the cold resistance of plants, and the growth state of the plants under cold stress is remarkably improved through overexpression of the PgEGY3 gene.

The invention discloses a cold response PaIBH1 protein and application of a coding gene thereof in enhancing cold stress resistance of plants. The cold response PaIBH1 protein is a protein PaIBH1 with an amino acid sequence as shown in SEQ ID NO: 2. Experiments prove that the gene for coding the protein PaIBH1, namely the gene PaIBH1, is overexpressed in wild type arabidopsis thaliana, so that the cold resistance of the arabidopsis thaliana can be improved; the improvement of the cold resistance shows that after cold treatment, the fresh weight of the overground part is increased, the wilting rate is reduced, the leaf membrane damage and electrolyte leakage are reduced, the photosynthetic ability is improved, the membrane lipid peroxidation damage is relieved, the active oxygen scavenging ability is enhanced and / or the active oxygen accumulation in the plant body is reduced. Therefore, the protein PaIBH1 can improve the cold resistance of the plants. The method has a very wide application prospect in the aspect of cold-resistant genetic improvement of crops or pasture.

The invention provides an application of an OsINV2 gene in regulation and control of rice cold stress resistance. A nucleotide sequence of the OsINV2 is shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. By revealing the key regulation and control effect of the vacuole sucrose invertase gene OsINV2 in rice low-temperature stress response, the molecular mechanism that the gene affects the cold resistance of plants by regulating the sugar metabolism balance in vacuoles is illustrated for the first time. The expression of the OsINV2 is regulated in a targeted manner through molecular marker-assisted breeding or a gene editing technology, so that the low-temperature survival rate in the seedling stage and the cold resistance in the booting stage of the rice can be remarkably improved, glumous flower degeneration and pollen abortion caused by low temperature are reduced, and meanwhile, the limitation of the cold resistance of varieties on geographical distribution is broken through; a theoretical support and a technical path are provided for breeding and adaptive popularization of cold-resistant rice varieties.

The invention provides a cutting method and a cutting device, and relates to the technical field of precision cutting machining. The cutting method comprises the steps that a battery piece is installed and fixed, the battery piece is provided with a preset cutting path, and the battery piece has preset bending moments on the two sides of the preset cutting path; laser is used for cutting an induction groove along a preset cutting path of the battery piece; and spraying a cooling medium into the inducing groove. According to the cutting method, the preset bending moment can be applied to the two sides, located on the preset cutting path, of the battery piece to generate the tensile stress, then the cooling medium with the preset pressure is sprayed to the induction groove in a matched mode, the tensile stress is increased when cold and hot stress cracking is generated, and the cracking efficiency of the battery piece is improved; obviously, by using the cutting method provided by the invention, the processing time of a cold and hot stress direct splitting technology after the laser induction groove is applied can be shortened, and the working efficiency is higher.

The invention discloses a low-carbon and energy-saving fattening pig house air conditioner control method which comprises the following steps: S1, constructing a grid digital twinborn model of a pig house, and initializing the grid digital twinborn model by using sensor data; s2, based on the gridding digital twin model, physical flow field simulation is executed, and temperature field distribution in the hog house is obtained; s3, temperature field distribution is analyzed, a temperature anomaly region needing to be regulated and controlled is recognized, and the attribute of the temperature anomaly region is quantified; s4, determining a target regulation and control unit and calculating regulation and control parameters of the target regulation and control unit based on the attribute of the temperature abnormal region; and S5, executing regulation and control and updating the digital twin model based on feedback data to form predictive closed-loop control based on physical simulation. By eliminating a local hot / cold stress area, the uniformity and stability of the hog house hot environment are greatly improved, meanwhile, a precise on-demand regulation and control mechanism is achieved, global excessive air supply is avoided, and substantive low-carbon and energy-saving effects in the operation process are achieved.

The invention relates to the technical field of rice gene engineering, in particular to application of a rice gene OsbZIP21 in regulation and control of cold resistance. The invention discloses an application of a rice gene OsbZIP21 in regulation and control of cold resistance. The nucleotide sequence of the OsbZIP21 gene is as shown in a sequence table SEQ ID NO: 1, and the coded amino acid sequence is as shown in a sequence table SEQ ID NO: 2. According to the invention, the rice OsbZIP21 gene is edited by adopting a CRISPR / Cas9 gene editing technology, the expression of the encoding protein of the rice OsbZIP21 gene is silenced, and two forms of cold-resistant rice mutants are obtained. Experimental results show that under the condition of cold stress, the survival rate of the two obtained rice mutants is obviously higher than that of wild rice mutants, and it is indicated that the OsbZIP21 gene plays an important role in regulation of the cold tolerance of rice. The invention provides new resources and technical means for cold-resistant breeding of rice and other plants.

The invention discloses an application of an alfalfa gene MsCBF4 in improving the cold resistance of plants, and belongs to the technical field of gene engineering. In order to excavate a gene with a clear anti-freezing function from medicago sativa, the medicago sativa gene MsCBF4 is successfully cloned, and the expression of the MsCBF4 gene is found to be induced by cold stress and freezing stress through the expression specificity and space-time expression analysis of the gene in the medicago sativa. Besides, a plant overexpression vector containing the alfalfa MsCBF4 gene and recombinant agrobacterium are constructed, transgenic arabidopsis thaliana overexpressing the MsCBF4 gene is obtained through a genetic transformation experiment, freezing tolerance analysis on the transgenic arabidopsis thaliana overexpressing the MsCBF4 gene shows that the overexpression of the MsCBF4 gene enhances the tolerance of the arabidopsis thaliana to freezing stress, and the transgenic arabidopsis thaliana overexpressing the MsCBF4 gene can be used for preventing freezing stress. Therefore, the MsCBF4 gene can be used for improving the cold resistance of the plant.

The invention provides a pogostemon cablin cold stress response transcription factor and application thereof in resisting cold and increasing pogostemon cablin alcohol content, and belongs to the technical field of gene engineering. The terpene metabolism cold response transcription factor PatbZIP6 and / or PatMYB5 is obtained through screening, the transcription factor obtained through screening can remarkably improve synthesis accumulation of patchouli alcohol through a genetic transformation method, the cold resistance of patchouli is improved, and the transcription factor can be used for breeding new varieties of patchouli.

The application of exogenous complex control measures in reducing tobacco low temperature early flowering, after tobacco transplanting, before the arrival of low temperature cold damage, exogenous complex measures solution is sprayed; the exogenous complex chemical measures solution is the mixed solution of magnesium sulfate and salicylic acid. The invention uses low temperature sensitive tobacco variety Yunyan 87 as experimental material, compares and analyzes the changes of leaf phenotype, physiology and biochemistry indexes of Yunyan 87 seedlings under low temperature stress and various exogenous material treatment, and confirms the role of the complex measures in resisting cold stress. The low temperature adversity resistance of tobacco seedling stage plants is improved by using suitable concentration magnesium sulfate to spray the tobacco seedlings, so that the growth state of the tobacco seedlings subjected to the low temperature adversity stress of the reverse spring cold tends to be normal, the frost injury and frost death phenomenon of the tobacco seedlings after the cold stress is reduced, the cold resistance of the tobacco seedling stage is improved, the early flowering in the later period is prevented, and the operation is simple, the cost is low, and the effect is obvious.

The invention discloses a pennisetum purpureum PpDREB1D-like gene and application thereof in improving cold resistance and biomass of plants, and relates to the technical field of plant genetic engineering. The PpDREB1D-like gene derived from the pennisetum purpureum is cloned and functionally identified for the first time, and experimental verification shows that the PpDREB1D-like gene can increase the cold resistance of plants, the growth state of the plants under cold stress is remarkably improved through overexpression of the PpDREB1D-like gene, the cold resistance of the plants is remarkably reduced through silence of the PpDREB1D-like gene, and the PpDREB1D-like gene can be used for improving the cold resistance of the plants. A molecular foundation is laid for popularization and planting of pasture and energy crops in cold regions, and the method has remarkable agricultural production and ecological value.

The invention discloses an application of a ClBZR1L gene in enhancing the cold resistance of watermelons by promoting proline accumulation. The invention belongs to the technical field of plant genetic engineering. The CDS sequence of the ClBZR1L gene is as shown in SEQ ID NO. 1. Experiments show that when a recombinant vector for constructing the silent ClBZR1L gene is transferred into a watermelon plant, the proline content of the silent plant under cold stress treatment is remarkably reduced compared with that of a control group, and the cold resistance is reduced. A recombinant vector for constructing the over-expression ClBZR1L gene is transferred into a watermelon plant, the proline accumulation amount of the over-expression plant under cold stress treatment is remarkably increased, the cold resistance is enhanced, and a gene resource is provided for watermelon cold-resistant molecular breeding. Besides, the invention also discloses direct combination of the ClBZR1L gene and a proline synthesis rate-limiting enzyme coding gene ClP5CS1 gene promoter, and the expression of the coding gene is up-regulated, so that synthesis of proline under cold stress is promoted, and a theoretical basis is provided for in-depth study on cold-resistant gene pathways.

The invention provides a breeding house automatic control ventilation system and a breeding house, the breeding house automatic control ventilation system comprises an air bellow and a control module, the air bellow is arranged in the upper space of the breeding house and is provided with a first air port and a second air port, the first air port is arranged in the side wall of the air bellow and is used for blowing air in the direction away from animals, and the second air port is arranged in the side wall of the air bellow; the second air opening is formed in the bottom wall of the air bellow and can blow air towards the animal body surfaces in the breeding house; the control module is in communication connection with the air bellow and can control opening or closing of the first air opening and the second air opening based on the current season. Therefore, the second air port can be closed to avoid formation of body surface wind and animal cold stress in the season with low air temperature, and the second air port can be opened to provide body surface wind and avoid animal heat stress in the season with high air temperature, so that the body surface comfort of the animal is further improved, the possibility that the animal gets ill is reduced, and the animal quality is improved. And large-scale economic loss of the farm is also avoided.

The invention relates to the technical field of plant genetic engineering, in particular to application of a rice OsERF141 protein and a coding gene thereof in improving low-temperature tolerance of plants. Experiments show that the low-temperature resistance of an OsERF141 function deletion mutant strain is remarkably reduced, and the low-temperature resistance of an overexpressed OsERF141 transgenic strain is remarkably improved. The invention provides a new gene resource for the cold resistance problem of rice, and has important significance for the molecular mechanism research of low-temperature stress and the cultivation of new low-temperature-resistant varieties.

The application provides application of a calcium sensing protein ShCBL1 gene and belongs to the technical field of biology.The application overexpresses or superexpresses the ShCBL1 gene in sugarcane or rice, so that the content of proline and superoxide dismutase in the sugarcane or rice is increased, the content of malondialdehyde is reduced, and the cold resistance is improved.Researches show that when the ShCBL1 is superexpressed or overexpressed in plants, the cold stress related physiological indexes such as proline, superoxide dismutase (SOD) and malondialdehyde (MDA) can be significantly affected, so that the cold resistance of the rice and the sugarcane is enhanced.ShCBL1 as a positive regulator of cold resistance regulation can be used for molecular breeding of cold resistance varieties.

This invention provides a cold adaptation regulatory gene RAF3 / RAF6 This invention relates to the field of genetic engineering technology and its applications. Specifically, it provides a cold adaptation regulatory gene. RAF3 / RAF6 , wherein RAF3 The genome sequence is shown in SEQ ID No. 1. RAF6 The genome sequence is shown in SEQ ID No. 2. This invention also provides the... RAF3 / RAF6 The encoded protein, and its role in reducing RAF3 / RAF6 By reducing the expression level of a protein or decreasing its activity or content, the cold tolerance and photosynthetic efficiency of plants can be improved, meaning that under cold conditions, RAF3 and / or RAF6 It reduced photosynthesis while enhancing the expression of cold-response genes, ultimately inhibiting plant growth and promoting plant adaptation to cold stress.

The application discloses a breeding method and feed for enhancing cold stress resistance of chickens and belongs to the technical field of chicken breeding. The breeding method comprises an environment adaptation process and a feeding adaptation process. The specific operation is as follows: broiler chickens are fed at an environment of 35 DEG C for 3 days, the temperature is lowered to 33 DEG C for feeding for 4 days, then the temperature is lowered by 3 DEG C, the temperature is lowered at a rate of 1 DEG C per two days for feeding, when the temperature is lowered to 26 DEG C, the temperature is lowered at a rate of 2 DEG C per two days to 16 DEG C, and the temperature is kept constant for 10 days to complete the environment adaptation process of the broiler chickens; the feeding adaptation is simultaneously performed during the environment adaptation process, when the feeding temperature is lowered to 16 DEG C, the ordinary feed is fed while the anti-cold stress feed is added once a day, the feeding is continuously performed until before the temperature is suddenly lowered, the feeding frequency of the anti-cold stress feed is increased to twice a day or three times a day after the temperature is suddenly lowered, and the feeding adaptation is completed after the feeding is continuously performed for at least one week, so that the influence of low temperature on the chicken breeding industry is reduced.

The invention discloses a multi-stage heat recovery device for cultivation based on self-circulation air defrosting, which is characterized in that a first-stage heat exchange core and a second-stage heat exchange core are respectively arranged in a shell, and a first switching assembly and a second switching assembly are respectively arranged on the side surfaces of the first-stage heat exchange core and the second-stage heat exchange core in the shell; and each air inlet, each air outlet and the exhaust air outlet side of the second-stage heat exchange core are respectively provided with a temperature sensor. According to the device, a self-circulation air defrosting method is adopted, energy consumption is saved, operation is stable, meanwhile, the device is friendly to the animal house environment, excessive cooling capacity cannot be introduced, and the environment is greatly changed; meanwhile, the two switching assemblies are simple in structure and can be controlled by one motor; a double-core switching method in the device ensures continuous heat recovery, continuous work can be achieved in severe cold areas, sudden cooling of the environment of an animal house is avoided, and cold stress of livestock can be effectively prevented.

Compositions and methods for creating plants exhibiting enhanced resistance to abiotic stresses, especially cold stress are disclosed.

This utility model discloses a partial air intake ventilation system for the roof of a wide-body livestock shed, belonging to the field of livestock shed ventilation technology. It includes an air intake device at the front of the shed, a ventilation ceiling installed on the top of the shed, and an exhaust device at the rear of the shed. The roof area above the ventilation ceiling is connected to the air intake device, and the breeding area below the ventilation ceiling is connected to the exhaust device. Multiple ceiling air inlets connecting the roof area and the breeding area are installed on the ventilation ceiling. This ventilation system, by installing a ventilation ceiling on the top of the shed, allows cold air to first enter above the ventilation ceiling and then be evenly distributed to the breeding area from top to bottom through the ceiling air inlets. At this time, the heat inside the shed preheats the cold air above the ventilation ceiling, effectively improving the temperature uniformity and air freshness of the wide-body shed, avoiding cold stress caused by uneven airflow, and achieving precise air delivery to the breeding area, creating a more comfortable growing space for poultry and livestock.

The application discloses a kind of dairy cow cold stress multistage fuzzy comprehensive evaluation method, comprising the following steps: step (1): using analytic hierarchy process, from warm environment (temperature, relative humidity, wind speed and illumination), physiological factors (respiratory rate, body surface temperature) and air quality (NH3, CO2, PM 10 ) 3 dimensions construct dairy cow cold stress evaluation index system;Step (2): establish the judgment matrix of each index layer, and make consistency check, if meet the consistency requirement, then determine the weight of each index layer;Step (3): if it does not meet the consistency check, use genetic algorithm for testing and correcting the judgment matrix, the weight of each index layer is calculated by crossing, mutation and other operations;Step (4): the degree of cold stress of dairy cow is obtained by multistage fuzzy comprehensive evaluation;The application combines environmental parameters with dairy cow physiological parameters, and can realize comprehensive and accurate evaluation of dairy cow cold stress.

A cold stress evaluation method for livestock in cold and arid regions, characterized in that the evaluation method comprises the following steps: preprocessing related data, establishing an evaluation index system; constructing a cold stress fuzzy comprehensive evaluation model; applying the cold stress fuzzy comprehensive evaluation model, analyzing the cold stress comprehensive evaluation results, and obtaining accurate evaluation results of the cold stress of livestock; the specific cold stress conditions of the livestock group and different individuals in the livestock group can be accurately judged, thereby providing reasonable guidance and decision-making for the cold region farm in terms of warmth, ventilation, feed adjustment and medication.

The application discloses use of Bacteroides thetaiotaomicron in preparation of products for reducing cold stress response of ruminants. It is found that feeding Bacteroides thetaiotaomicron to ruminants, especially young calves, can effectively reduce the cold stress response of the ruminants.

The invention belongs to the field of crop stress resistance regulation and control, and discloses a method for improving cold stress resistance of rice in the seedling stage and application of the method, particularly a method for improving cold stress resistance of rice seedlings through exogenous application of 5-methoxyl-tryptamine and application of the 5-methoxyl-tryptamine in agricultural production, the use concentration of 5-methoxyl-tryptamine is 0 [mu] mol / L, 1 [mu] mol / L, 10 [mu] mol / L or 50 [mu] mol / L, and the use concentration of 5-methoxyl-tryptamine is 0 [mu] mol / L, 1 [mu] mol / L, 10 [mu] mol / L or 50 [mu] mol / L; the plant height, the root length, the fresh weight, the dry weight, the dry matter content, the chlorophyll content and the hydrogen peroxide content of rice to which 1 mu mol / L 5-methoxytryptamine is applied are all remarkably improved compared with rice to which treatment is not applied, the effect is particularly the best under the cold stress condition, damage of cold stress to rice in the seedling stage can be relieved, and therefore the rice yield is increased. Therefore, the invention provides a theoretical basis for researching the mechanism of improving the cold stress resistance of the rice in the seedling stage by the 5-methoxytryptamine, and provides practical guidance for researching the cold tolerance of plants.

The invention relates to an application of a corn ZmXTH27 gene in improving the cold resistance of plants. The ZmXTH27 gene encodes a protein of a xyloglucan endotransglycosidase / hydrolase (XTH) family, the gene expression level of the ZmXTH27 gene is remarkably increased under low-temperature stress, and a mutant zmxth27 of the gene shows a low-temperature sensitive phenotype. The ZmXTH27 gene is transformed into arabidopsis thaliana and is subjected to functional verification, and compared with wild type arabidopsis thaliana, the tolerance of an arabidopsis thaliana plant over-expressed with the ZmXTH27 gene to cold stress is remarkably enhanced. According to the invention, more theoretical bases are provided for corn cold resistance research, and gene resources and biotechnological means are provided for subsequent cultivation and germplasm resource innovation of new cold-resistant corn strains.

The invention discloses application of IL17D in rescuing oocytes damaged by cold stress, a culture solution capable of rescuing oocytes damaged by cold stress, application of the culture solution in rescuing oocytes damaged by cold stress, application of the culture solution in improving quality of somatic cell cloned embryos, and application of the culture solution in rescuing oocytes damaged by cold stress. The invention further discloses a method for improving the in-vitro maturation quality of the oocytes damaged by cold stress. The invention also discloses an application of IL17D in reducing the active oxygen level in the oocytes damaged by cold stress, an application in improving the glutathione content in the oocytes damaged by cold stress and an application in improving the antioxidant gene expression in the oocytes damaged by cold stress. The IL17D is added into the in-vitro maturation culture solution of the oocytes, so that the damage of cold stress to the porcine oocytes can be saved, and a new solution and a new idea can be provided for solving the problems that the in-vitro maturation quality of the oocytes is influenced and the embryo quality is further influenced due to the fact that the oocytes are damaged by the cold stress in winter.

The utility model discloses a cold stress experiment temperature control box relates to temperature control box technical field, including main box body and the temperature control system of setting up on it, one side fixed connection of main box body has the additional position box main box body of experiment animal access main box body is equipped with the closed gas mechanism of opening and closing main box body and additional position box connecting position department and remaining low temperature gas in main box body, and the closed gas mechanism includes the side position disc of fixed connection on main box body, is equipped with multiple sets of triangle seal seat on side position disc, and is equipped with the ring seal subassembly of the drive multiple sets of triangle seal seat synchronous deflection to seal or open passageway groove on side position disc, the utility model discloses a closed gas mechanism is set up, when replacing test object, can effectively remain most low temperature air in main box body, and then greatly reduced the cold air loss of the test object replacement, thereby improves the experiment efficiency, reduced the operation cost of equipment, makes the cold stress experiment more energy -efficient.

The invention discloses a CAPS molecular marker developed based on a ZmMYB24 gene and related to salt resistance and low temperature resistance of corn and application of the CAPS molecular marker. Sequence variation of a ZmMYBR24 gene coding region induced and expressed by salt stress and low-temperature stress in different corn homozygous inbred lines is analyzed, correlation analysis is carried out on salt tolerance and low-temperature resistance of the corn homozygous inbred lines, correlation sites are mined, and then the CAPS molecular marker related to salt tolerance and low-temperature resistance of corn is developed and named as CAPs814. The molecular marker CAPs814 is used for marker-assisted selection, the average detection efficiency in identification of maize inbred lines with medium salt resistance and medium low temperature resistance or above is 80.56% and 86.11% respectively, and the molecular marker CAPs814 provides a new technical means for screening maize materials with medium salt resistance and low temperature resistance.

This invention discloses a type of alfalfa MsCYP Genes and their applications in salt and cold tolerance. This invention, based on phenotypic and genome association analysis, screened a gene in alfalfa that responds to salt and cold stress. MsCYP The MsCYP The nucleotide sequence is shown in SEQ ID No. 1, and the amino acid sequence is shown in SEQ ID No. 2. Further overexpression in alfalfa hairy roots... MsCYP The results showed that under salt stress or a low temperature of 4°C, overexpression MsCYP The hairy roots of the gene are longer than those of the wild type, and the plant exhibits greater tolerance to salt and low-temperature stress than the wild type, indicating that the gene is overexpressed. MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsCYP MsC Genes can enhance the salt and cold tolerance of alfalfa. This invention provides theoretical and practical support for the breeding and widespread promotion of salt- and cold-tolerant alfalfa varieties.

The present invention discloses a composite plant growth regulator and its application in improving the cold tolerance of cotton seedlings. The present invention firstly compounds 2,4-epibrassinolide and prohexadione calcium, and sprays the composite growth regulator obtained by the compounding on cotton seedlings after cold stress. The results show that compared with the control, the composite growth regulator effectively increases the relative chlorophyll content, plant height, dry weight and fresh weight of stems and leaves of cotton seedlings in the recovery stage, indicating that it can quickly restore the growth of leaves and stems of cotton seedlings after cold stress, promote the accumulation of substances in cotton seedlings after cold stress, and explore the optimal ratio (EBR 0.04 mg / L+PC 30 mg / L). The composite growth regulator of the present invention eliminates the adverse effects of cold stress to a certain extent, ensures the healthy growth of cotton seedlings, effectively solves the problem of cotton agricultural production in Xinjiang, and is of great significance for ensuring high and stable yield of cotton.