7 results about "Freezing tolerance" patented technology

The invention discloses an application of an alfalfa gene MsCBF4 in improving the cold resistance of plants, and belongs to the technical field of gene engineering. In order to excavate a gene with a clear anti-freezing function from medicago sativa, the medicago sativa gene MsCBF4 is successfully cloned, and the expression of the MsCBF4 gene is found to be induced by cold stress and freezing stress through the expression specificity and space-time expression analysis of the gene in the medicago sativa. Besides, a plant overexpression vector containing the alfalfa MsCBF4 gene and recombinant agrobacterium are constructed, transgenic arabidopsis thaliana overexpressing the MsCBF4 gene is obtained through a genetic transformation experiment, freezing tolerance analysis on the transgenic arabidopsis thaliana overexpressing the MsCBF4 gene shows that the overexpression of the MsCBF4 gene enhances the tolerance of the arabidopsis thaliana to freezing stress, and the transgenic arabidopsis thaliana overexpressing the MsCBF4 gene can be used for preventing freezing stress. Therefore, the MsCBF4 gene can be used for improving the cold resistance of the plant.

The invention discloses an application of gossypose in improving freezing resistance of beet, and belongs to the technical field of plant freezing stress. The purpose of the invention is to improve the freezing resistance of beet. A method for resisting freezing stress of beet comprises the following steps: spraying a raffinose solution at the external source of beet seedlings in an environment of-4 DEG C, and culturing the beet seedlings. An effective method is provided for improving the freezing resistance of the beet.

This application discloses the application of the BnaCT3 gene and its SNP molecular markers in regulating the cold resistance of rapeseed. The nucleotide sequence of the BnaCT3 gene is shown in SEQ ID NO.1. Overexpression of the BnaCT3 gene in rapeseed significantly improves the survival rate of plants under low-temperature stress, reduces electrolyte leakage rate and malondialdehyde and hydrogen peroxide content, increases proline content and antioxidant enzyme activity, and enhances the cold resistance of rapeseed. Furthermore, this application identifies two SNP sites located in the promoter region of the BnaCT3 gene, whose specific base combinations constitute H1 and H2 allele combinations. Rapeseed materials carrying the H2 allele exhibit significantly better cold resistance than those carrying the H1 allele, and all currently promoted varieties lack the H2 allele. This application provides effective gene resources and molecular markers for the genetic improvement of rapeseed cold resistance, which can be used for overexpression transgenic breeding and marker-assisted selection breeding.

The invention provides application of glutathione in enhancing low-temperature freeze injury tolerance of tea trees and a method thereof, and relates to the technical field of plant protection. According to the method, 0.5-2.0 mmol / L glutathione solution is sprayed on the leaf surfaces of the tea trees to enhance the low-temperature freeze injury tolerance of the tea trees. The invention further provides a method for enhancing the low-temperature freeze injury tolerance of the tea trees, low-temperature stress of the low-temperature freeze injury to the tea trees can be remarkably relieved in a short time by spraying glutathione, oxidation damage caused by stress is relieved, the tea trees are prevented from being hindered in growth, even dead and the like, the stress resistance and viability of crops are improved, and the survival rate of the crops is increased. And the treated tea leaves are free of chemical substance residues, so that the safe production and the quality of the tea leaves are guaranteed.

The invention relates to a preparation method of a medical gel dressing with anti-freezing performance and photo-thermal rewarming performance, belongs to the field of hydrogel composite materials, and is applied to prevention and treatment of frostbite of field operation personnel in high-cold regions under emergency conditions. The preparation method comprises the following steps: preparing hydrogel with a double-network structure from chitosan (CS) and acrylamide (AM), stripping and layering Ti3AlC2 to obtain an MXene nanosheet as a photothermal conversion component for rewarming, betaine as an anti-freezing component, N 'N-methylene bisacrylamide as a cross-linking agent and ammonium persulfate as an initiator, and preparing CS / PAM / MXene / Betaine / AgNPs gel through a one-pot method. According to the invention, the good photo-thermal conversion effect of MXene is utilized, so that the gel dressing for frostbite is endowed with photo-thermal rewarming performance; due to the synergistic interaction of the betaine ammonium sulfate, the good anti-freezing tolerance is also shown; the nano-silver is loaded in the gel matrix, so that the gel can be endowed with remarkable antibacterial activity. The medical anti-freezing and rewarming gel dressing has the advantages that the medical anti-freezing and rewarming gel dressing is expected to be used for emergency prevention and treatment under the condition of frostbite of field operating personnel in high-cold regions.

The application belongs to the technical field of fish feed additives, and discloses a fish feed for improving the quality of fish meat after freezing and thawing and application thereof. The fish feed comprises a basic feed and theaflavins, and the mass percentage of the theaflavins in the fish feed is 0.02-0.06%. The application is to improve the anti-freezing tolerance of grass carp muscle from the source through feeding intervention, effectively inhibit the growth of ice crystals and lipid oxidation in the subsequent freezing and thawing process, significantly improve the texture properties (such as chewiness and cohesiveness) of muscle, and maintain the integrity of cell structure. The application method can be quickly popularized, has the advantages of simple operation, low cost, significant improvement effect and the like, and provides an effective way for solving the quality deterioration problem of aquatic products in the freezing, storage and transportation process.

The invention belongs to the technical field of fish feed additives, and discloses a fish feed for improving the quality of frozen and thawed fish meat and application of the fish feed. The fish feed comprises a basal feed and theabrownin, wherein the mass percentage of the theabrownin in the fish feed is 0.02-0.06%. According to the application, through feeding intervention, the anti-freezing tolerance of grass carp muscles is improved from the source, ice crystal growth and lipid oxidation can be effectively inhibited in the subsequent freezing and thawing process, the texture characteristics (such as chewiness and cohesiveness) of the muscles are remarkably improved, and the cell structure integrity is kept. The application method can be quickly popularized, has the advantages of simplicity in operation, low cost, remarkable improvement effect and the like, and provides an effective way for solving the problem of quality degradation of aquatic products in the freezing storage and transportation process.