12 results about "Theabrownin" patented technology

The present application provides a composition with lipid-lowering and weight-reducing functions, which has clear components and controllable quality. The composition comprises Pu'er tea extract, wherein the Pu'er tea extract contains 10-90% theabrownine by weight, preferably also contains theophylline, the weight ratio of the theabrownine and theophylline is 10:1-60:1, and contains 0-15% tea polyphenols by weight. The theophylline can be selected from exogenous theophylline or theophylline contained in the Pu'er tea extract. The composition with lipid-lowering and weight-reducing functions of the present application also comprises Poria cocos extract; the weight ratio of the Pu'er tea extract to the Poria cocos extract is 1:3-3:1 based on the weight of theabrownine contained in the Pu'er tea extract. The composition of the present application has the advantages of clear effective components, simple preparation process, controllable quality, and stable lipid-lowering and weight-reducing effects.

The present application relates to the technical field of microbial feed additive, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the following steps: taking Lactobacillus plantarum and / or Enterococcus faecalis as a bacterial strain to activate and expand culture, taking agricultural and sideline product waste as a substrate to obtain mature fermentation liquor through deep liquid fermentation; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide to obtain a semi-dry granule core through granulation and low-temperature pre-drying; and coating the semi-dry granule core with a κ-carrageenan / konjac glucomannan / plant sterol ester composite gel, and drying and packaging to obtain a finished product.The double essential oil, chitosan and theabrownin jointly form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; the porous silicon dioxide actively maintains low water activity of the granule to prevent the lactic acid bacteria from recovering in advance; and the product has high viable count, strong heat and acid resistance, and excellent storage stability.

The invention discloses a method for treating Pu'er tea with fermentation liquor. The method comprises the following steps: naturally fermenting honey, first-spring fresh tea leaves and mountain spring water serving as raw materials according to a mass ratio of 1: 10: 3 to prepare the fermentation liquor; and adding mountain spring water accounting for 50% of the weight of the sun-withered raw tea into the sun-withered raw tea, adding 0.5% of the fermentation liquid into the mountain spring water, uniformly mixing, and carrying out pile fermentation for 55-60 days to obtain the fermented Pu'er tea. Microbiomics and metabonomics analysis verifies that a dominant flora dominant by lactic acid bacteria and bacillus is rapidly established in the early stage of fermentation, efficient conversion of polyphenols is promoted, the content of theabrownin is increased by 33% or above, and volatile aroma components are remarkably rich.

The invention relates to the technical field of microbial feed additives, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the steps that lactobacillus plantarum and / or enterococcus faecalis are / is used as strains for activation and expanding culture, agricultural and sideline product waste is used as a substrate for liquid-state deep fermentation, and mature fermentation liquor is obtained; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide, granulating, and pre-drying at low temperature to obtain a semi-dry particle core; and coating with kappa-carrageenan / konjac glucomannan / phytosterol ester composite gel, drying and packaging to obtain a finished product. The double essential oils, the chitosan and the theabrownin are combined to form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; porous silicon dioxide actively maintains low water activity of particles to prevent lactic acid bacteria from being recovered in advance; the product has the advantages of high viable count, strong heat and acid resistance and excellent storage stability.

This invention discloses a method for preparing Ganoderma lucidum Liubao tea, belonging to the field of deep tea processing technology. Addressing the limitations of traditional Liubao tea's single fermentation method and limited functional components, this invention blends traditional Liubao tea raw materials with 5% Ganoderma lucidum ultrafine powder, undergoing a secondary light fermentation at 28-32℃ and 85% humidity for 7-10 days to form a stable microbial system. The final product is then fixed using a gradient drying technique. This method results in a finished product with both aged aroma and a sweet, mellow taste, increasing the theabrownin content by 15-20%, and enriching it with Ganoderma lucidum triterpenoid active ingredients. Breaking through traditional processes, this method achieves targeted transformation of tea's functional components, providing dual health benefits of immune regulation and antioxidant effects.

The invention discloses a theabrownin enhanced freeze-dried weight-losing granule as well as a preparation method and application thereof. Comprises: theabrownin derived from a tea extract of dark tea and / or jasmine tea; an alpha-amylase inhibitor; a catechin-based substance; dietary fibers; a sweetener; wherein based on the total dry weight of the composition, the weight ratio of the theabrownin to the tea polyphenol in the tea extract is (1: 8)-(1: 20). According to the theabrownin enhanced freeze-dried weight-losing granules and the preparation method and application thereof, the freeze-dried granules have a loose porous structure and can be rapidly disintegrated and dissolved when meeting water, and the solution is uniform, stable and free of precipitation; key active ingredients (tea polyphenol, theabrownin, epigallocatechin gallate and an alpha-amylase inhibitor) are effectively protected in the production process, and the final product is high in content and clear in proportion, so that a synergistic fat reducing effect is favorably exerted; meanwhile, the product is good in stability and convenient to store and carry.

This invention discloses a *Aspergillus ryukyu* strain and its applications. The *Aspergillus ryukyu* strain is named *Aspergillus ryukyu* P001, with accession number CGMCC NO.42119, deposited on July 3, 2025, at the China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. The *Aspergillus ryukyu* strain provided by this invention can efficiently secrete catechol 1,2-dioxygenase, significantly improving the degradation efficiency of phenolic substances. When the strain provided by this invention or its produced catechol 1,2-dioxygenase ferments tea (such as ripe Pu-erh tea), it can accelerate the degradation of tea polyphenols, catechins, and other substances, promote the formation of theaflavins, resulting in a reddish-brown tea liquor with a mellow taste and a shortened fermentation cycle.

The application discloses a theabrownin carbon quantum dot preservative film with monitoring and antibacterial effects, and a preparation method and application thereof. The preparation method comprises the following steps: (1) theabrownin preparation, (2) carbon quantum dot preparation, (3) theabrownin carbon quantum dot, citric acid and graphite phase carbon nitride composite electrospinning nanofiber film preparation and heat-induced esterification curing. The prepared carbon quantum dots are closely combined with graphite phase carbon nitride through an electrostatic field, and the antibacterial efficiency is synergistically improved, the photo-thermal performance is significantly better than that of theabrownin carbon quantum dots, and active oxygen ability is generated. The application of the preservative film in fruit preservation has the dual effects of fruit freshness indication based on fluorescence quenching and antibacterial preservation, and the fruit storage period can be prolonged by 100% at room temperature.

The application belongs to the embedding and slow-release technical field of protein, and particularly relates to a composite coacervate based on whey protein isolate and theabrownine as well as a preparation method and application thereof. The theabrownine and the whey protein isolate are respectively dissolved in an aqueous solution, and the whey protein isolate-theabrownine composite coacervate is prepared by controlling the ratio of the whey protein isolate aqueous solution and the theabrownine aqueous solution, the pH value of the system and the ionic strength. Compared with the prior art, the application takes electrostatic interaction as a driving force, realizes the preparation of the whey protein isolate-theabrownine composite coacervate by controlling process parameters and adjusting the intramolecular and intermolecular electrostatic interaction, and provides important technical support for preparing novel food ingredients with various structures and rich functions.

The invention relates to an aspergillus nidulans strain with high yield of theabrownin and application thereof. The aspergillus nidulans strain is preserved in China General Microbiological Culture Collection Center on December 1, 2025, the preservation name of the aspergillus nidulans strain is Aspergillus nidulans A-17, and the preservation number of the aspergillus nidulans strain is CGMCC (China General Microbiological Culture Collection Center) No. 42334. The aspergillus nidulans provided by the invention has efficient theabrownin production capacity, does not produce mycotoxin, and can be applied to theabrownin industrial production; meanwhile, the strain can also be inoculated with tea leaves and a tea extracting solution for fermentation, and is used for industrially producing high-theabrownin dark tea and high-theabrownin instant tea. In addition, the strain can significantly reduce the delicate flavor and astringent taste of tea leaves, endows the tea leaves with stale flavor and mellow taste, and can be used for producing high-quality dark tea. The aspergillus nidulans provided by the invention has strong capability of producing theabrownin, has no safety risk, provides high-quality microbial germplasm resources for preparing theabrownin, high-theabrownin tea products and high-quality dark tea, and has a good industrial application prospect.

The invention relates to the technical field of biological medicine, and discloses a preparation method and application of a theabrownin-based anti-chemical liver injury medicine, and the preparation method comprises the following steps: extracting theabrownin with the molecular weight of 1kDa to 100kDa from post-fermented tea leaves; constructing a carbon tetrachloride induced mouse acute liver injury model and performing preventive administration; the in-vivo drug effect is evaluated through serum biochemical, histopathology and immunohistochemical indexes; an in-vitro macrophage inflammation model is constructed, and a molecular mechanism is analyzed by utilizing flow cytometry and an immunoblotting technology. Experiments prove that the theabrownin can remove active oxygen in cells, inhibit polarization of macrophages to a proinflammatory phenotype, and significantly reduce release of inflammatory factors such as IL-6 and the like by reducing expression of phosphorylated NF-kappa Bp65 and blocking nuclear translocation of the phosphorylated NF-kappa Bp65. The invention solves the problem of lack of molecular mechanism analysis in the prior art, and is suitable for preparing oral or injection medicines for treating toxic hepatitis in various chemical toxicants.

The invention provides a dark tea aging fermentation inoculant as well as a preparation method and application thereof, and belongs to the technical field of tea fermentation. The dark tea aging fermentation microbial inoculum disclosed by the invention comprises klebsiella and aspergillus; the mass ratio of the klebsiella to the aspergillus is 1: (1-10). The microbial agent is inoculated into finished tea leaves subjected to fixation, rolling, pile fermentation and drying treatment, constant-temperature and constant-humidity culture fermentation is performed for more than five months, catechin substances and soluble sugar in the tea leaves can be effectively converted, beneficial substances such as amino acids, theabrownin and flavonoids in the tea leaves are increased, and fermentation and quality improvement of the tea leaves are realized. The fermentation inoculant has a huge application value in the aspect of regulating and controlling fermentation of the dark tea.