434 results about "Bud" patented technology

The invention discloses a Chinese orchid flowering regulation gene CsFTL3 as well as an encoding protein and application thereof, and belongs to the field of plant molecular biology. The gene CsFTL3 for regulating and controlling flowering of Chinese cymbidium is separated from flower bud cDNA (complementary deoxyribonucleic acid) of a Chinese cymbidium variety 'Xiaoxiang', and 174 amino acids are encoded by the gene CsFTL3. Through construction of an expression vector of the CsFTL3 gene, overexpression in wild-type arabidopsis thaliana and heterologous overexpression of the CsFTL3 gene in the arabidopsis thaliana, it is found that bolting and flowering periods of the arabidopsis thaliana are significantly delayed, flower stems are significantly thickened and increased, the number of rosette leaves is significantly increased, and the growth period is prolonged, which indicates that the gene has the function of delaying the flowering time of the arabidopsis thaliana. The Chinese orchid flowering regulation and control gene can be used for molecular mechanism research of orchid flowering time, delay Chinese orchid flowering time, promote plant vegetative growth, prolong whole Chinese orchid viewing time and promote Chinese orchid genetic improvement and variety breeding.

The invention belongs to the technical field of agricultural production, and discloses an evaluation method for identifying the preliminary salt tolerance of foxtail millet in a germination period, which comprises the following steps: determining five key indexes of relative germination potential, relative germination rate, relative root length, relative bud length and relative germination index in the germination period of foxtail millet; the salt tolerance of the millet germplasm resources in the germination period can be preliminarily evaluated by establishing a regression equation to estimate the D value. The simple, convenient and scientific method for identifying the salt tolerance of the millet germplasm resources in the germination period is established, convenience is provided for screening salt-tolerant germplasm of millet, and the method is a premise for carrying out research on a salt-tolerant mechanism of millet.

The invention provides a method for sterile sowing and efficient propagation of cymbidium faberi. The method comprises the following steps: pretreatment of cymbidium faberi seeds, sterile germination of the cymbidium faberi seeds, protocorm proliferation, differentiation, seedling strengthening and acclimatization and transplantation. The pretreatment comprises the steps of refrigerating hybrid seeds of cymbidium orchid at 4 DEG C, performing secondary disinfection with sodium hypochlorite, performing dark culture for 10-30 days after sowing, and then performing light culture, and the germination culture medium is prepared by taking 1 / 4 MS as a basic culture medium and adding 100-200ml / L of coconut water, 0.5-1.0 mg / L of 6-benayl aminopurine, 0.1-0.5 mg / L of naphthylacetic acid, 0.5-2.0 g / L of peptone and 0.5-1.0 g / L of activated carbon. According to the method, the seed germination rate can be effectively increased, the 60d seed germination rate reaches up to 85%, the pollution rate is reduced to 5% or below, 50.0 g / L inositol, 2.0 mg / L glycine and 2.0 mg / L nicotinic acid are added into a differentiation culture formula, the differentiation rate can be effectively increased, the leaf bud differentiation rate reaches up to 90%, meanwhile, rooting can be induced, the growth time of cymbidium faberi from seeds to seedlings is greatly shortened, a large number of high-quality seedlings can be obtained within a short time, and the method is suitable for large-scale popularization and application. Technical data are accumulated for cymbidium resource protection and new variety cultivation.

The invention provides a rhodiola crenulata tissue culture and test-tube seedling acclimatization and transplantation method, and belongs to the technical field of plant tissue culture. The tissue culture method of the rhodiola crenulata comprises the following steps: (1) sterilizing rhodiola crenulata seeds, and performing germination culture to obtain adventitious buds; (2) transferring the adventitious buds obtained in the step (1) into an adventitious bud proliferation culture medium for adventitious bud proliferation culture to obtain cluster buds; and (3) transferring the cluster buds obtained in the step (2) into a seedling strengthening and rooting culture medium for seedling strengthening and rooting culture to obtain test-tube plantlets. The tissue culture and test-tube seedling acclimatization and transplantation method of rhodiola crenulata can provide a new method for large-area efficient cultivation of rhodiola crenulata, and has important application value for resource protection, development and utilization of rhodiola crenulata.

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

The invention discloses an application and a method for knocking out a poplar LUX1 gene in delaying growth stop or dormancy time under short day, and the poplar LUX1 gene is identified and obtained through amino acid sequence comparison with arabidopsis thaliana LUX. The poplar LUX1 gene is a protein for coding an amino acid sequence as shown in SEQ ID No.1. The LUX1 gene is knocked out through a CRISPR / Cas9 editing system, and the time that the poplar enters the dormancy period due to short sunlight in autumn is delayed, so that the poplar germplasm with long growth period and high biomass is obtained. After the LUX1 gene is knocked out, terminal bud growth stop and stem cambium activity reduction in the seasonal growth process of the poplar can be delayed, so that wood development is promoted. Deferring of the dormancy time under the autumn short-day condition has a great application prospect for prolonging the growth period of the trees so as to improve photosynthetic carbon sequestration and biomass accumulation.

The invention relates to the technical field of plant cultivation, in particular to a method for regulating and controlling precocious flowering of safflower by using a photoperiod. According to the method, safflower seedlings are cultured in a photoperiod environment of 20-hour illumination and 4-hour darkness, and flowering safflower plants are obtained. By adopting the method provided by the invention, the flower bud of the safflower can be successfully induced to differentiate in advance, the safflower can be promoted to bloom in advance, the growth state of the safflower is relatively good, and the method is an optimal photoperiod treatment condition for promoting the growth and development of the safflower and blooming in advance. The safflower treated by the method provided by the invention only needs 84 days from sowing to flowering, which is about 5 months earlier than that sown in autumn in the south in actual production, the growth cycle of the safflower is greatly shortened, and the plant height of the safflower plant is also higher than that of the safflower plant treated under the existing photoperiod condition and is two times that of the safflower plant treated under the photoperiod condition of 12h / 12h. The obvious difference is realized.

The invention belongs to the field of genetic engineering technology and transgenic breeding. The sequence of the chrysanthemum CmRAX2 gene is as shown in SEQ ID No.1, and the sequence of the encoded protein of the chrysanthemum CmRAX2 gene is as shown in SEQ ID No.2. Chrysanthemum leaf discs are infected through an agrobacterium-mediated method, the gene is transferred into chrysanthemum, a CmRAX2 gene trans-CmRAX2 gene chrysanthemum strain is obtained, and the result shows that the number of lateral buds, the plant height and the node number of the CmRAX2 gene trans-CmRAX2 gene chrysanthemum plant are increased, the appearance time and the rooting time of the lateral buds are advanced, the rooting ability of leaves is improved, and the number of buds is increased. Therefore, the chrysanthemum CmRAX2 gene disclosed by the invention has the functions of promoting the growth of lateral branches of the chrysanthemum and increasing the flower quantity, and has a good application prospect when being applied to plant character improvement.

The invention discloses a high-survival-rate grafting method for grafting pinus sylvestris to pinus koraiensis in arid regions, which comprises the following steps: selecting pinus sylvestris stock with the growth period of 4-6 years, flatly cutting below a terminal bud branch point to form a cross section, downwards and obliquely cutting twice from the cross section to form a triangular pyramid-shaped chimeric structure, cutting a semi-lignified pinus koraiensis scion with a terminal bud, and grafting the semi-lignified pinus koraiensis scion with the terminal bud to obtain the pinus sylvestris scion. After main buds are reserved and lateral buds are cut off, two oblique triangular xylem sections are obliquely cut out from the lower portion of spores through two cutters, and a double-slope wedge-shaped joint body is formed; when the rootstock and the scion are cut, the cutting blade is immersed in an aqueous solution containing a plant growth regulator and a bactericide; the double-slope wedge-shaped joint body is inserted into the triangular pyramid-shaped embedding structure, so that the scion medullary layer and the stock medullary layer are overlapped; wrapping the interface with a composite hydrogel film containing a temperature-sensitive polymer and an antibacterial component; shading treatment is carried out after grafting is completed. The method can significantly improve the survival rate of grafted seedlings.

The invention relates to the technical field of plant cell engineering, and particularly discloses a plant protoplast semi-fusion breeding method which comprises the following three stages of seedling material selection, preparation, fusion, induction and cultivation. A large number of fused plant seedlings are obtained through a series of operations and used for large-scale planting. The method comprises the following steps: selecting excellent varieties of a plant A and a plant B, respectively preparing the plant A and the plant B into protoplasts in a preparation process, culturing the protoplasts into corresponding calluses, and performing half-cell fusion on a fusion part to obtain mosaic calluses. Then, the callus of the plant A to be used as the root is induced to locally induce adventitious roots, the callus of the plant B to locally induce adventitious buds, and finally, breeding is carried out to obtain the super seedlings. According to the method, the grafting principle is combined, the degree and conditions of cell fusion are optimized, the breeding cost is reduced, meanwhile, it is ensured that the fusion body can stably express the excellent characters of parents at different parts, and agricultural large-scale production is facilitated.

The invention relates to the technical field of agricultural chemistry, and discloses a citrus sapling flower killing agent and a preparation method and application thereof, the flower killing agent comprises fluoroglycofen-ethyl, the dilution concentration of the fluoroglycofen-ethyl is 15000-25000 times, and the dosage form is 10% missible oil or 20% microemulsion. When the pesticide is used, the pesticide application time is the flower bud development S1 stage, and the spraying amount is 50-80 L / mu. According to the invention, the traditional application of fluoroglycofen-ethyl as a herbicide is broken through, the fluoroglycofen-ethyl is innovatively developed into the citrus sapling flower-killing agent, the functional boundary of pesticides is widened, and a brand new chemical regulation idea is provided for the flower control of woody plants; through fixed pesticide application period, concentration, environmental parameters and other elements, a reproducible'chemical pruning 'technical regulation is formed, the volatility of traditional empirical management is changed, and scientific and standardized development of citrus sapling management is promoted.

The invention discloses a method for establishing a cudrania tricuspidata stem efficient regeneration system, and belongs to the technical field of plant tissue culture. The establishment method comprises the following steps: cutting healthy Cudrania tricuspidata current-year twigs into stem segments, reserving a plump bud on each stem segment, carrying out preliminary disinfection, soaking in a disinfectant, carrying out ultrasonic treatment and disinfection, using the disinfected bud as an explant, and carrying out bud induction culture, cluster bud enrichment culture and rooting culture to obtain Cudrania tricuspidata in-vitro regenerated seedlings. By screening the components of the culture medium in each stage and optimizing the in-vitro rapid propagation process of the cudrania tricuspidata, the propagation efficiency of a rapid propagation system is improved, a large number of high-quality seedlings can be produced within a short period of time, the market demand is met, and rapid development of industrialization of the cudrania tricuspidata is promoted.

The object of the present invention is to provide a shaping and management machine for bunch-shaped fruits, comprising an opening and closing mechanism, at least two boxes are arranged on the opening and closing mechanism, and transmission mechanisms are arranged on the boxes. The opening and closing mechanism controls the two boxes to perform opening and closing movements, and the two boxes shape the flower buds through the transmission mechanism. The present invention drives the two boxes to close with a distance through the arranged opening and closing mechanism to form an elliptical shaping opening, and the plant branches will not be damaged during the shaping process. The transmission mechanism is used to control the rotation of multiple shaping wheels to remove the excess flower buds on the flower bud bunch outside the shaping opening, so that each flower bud bunch can be shaped evenly. After the shaped flower buds are pollinated, the fruit nutrition is sufficient, and the fruit particles are full and uniform in size. The management machine is handheld, and the user uses the management machine in a handheld manner to shape the flower bud bunch, and the shaping efficiency is high, which reduces the risk of missing the flower bud period due to low manual shaping efficiency and improves the quality of agricultural harvest.

The invention discloses a medicago sativa MsCBR1 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a medicago sativa gene MsCBR1 is found, and a medicago sativa mscbr1 mutant is constructed by knocking out the MsCBR1 gene. After cutting, compared with a control plant, the alfalfa mscbr1 mutant has the advantages that the number of regenerated branches, the number of leaves and the number of crown buds are reduced. Therefore, it is determined that the MsCBR1 gene has a regulation effect on regeneration of plants after alfalfa is cut. Therefore, the invention provides new theoretical support for cultivating multi-branch and high-yield medicago sativa materials.

The invention provides a callus induction, differentiation and plant regeneration method with rhododendron petals as explants and application of the callus induction, differentiation and plant regeneration method, and belongs to the technical field of tissue culture. S2, performing callus induction; s3, carrying out callus subculture; s4, adventitious bud differentiation; s5, adventitious root differentiation. In the method, the limiting conditions of each step and the components of the culture medium are specifically limited, and by adopting the culture medium and the related condition control method provided by the invention, the pollution rate of rhododendron petal tissue culture can be effectively reduced, the callus rate and differentiation rate of calluses can be improved, and the survival rate of regenerated plants can be further improved.

The invention belongs to the technical field of crossbreeding, and particularly relates to a castration hybridization method of amphoteric asparagus. The method comprises the following steps: selecting an amphoteric asparagus in a bud pre-mature period as an asparagus maternal plant; petals and stamen groups of flower buds on the maternal plants are pinched off on the pollination day, and bare stigmas are left; picking mature and pollinated male flowers as male parent flowers on the pollination day; adhering the collected male parent pollen to the castrated female parent flower stigma after 4 p.m. at the temperature of 25-35 DEG C in clear weather from April to June every year, bagging and isolating to obtain homozygous seeds. The efficient method is provided for hybridization of the amphoteric asparagus for the first time, variety mixing in the asparagus hybrid seed production process is eradicated, the purity of the seeds is guaranteed, and the consistency of seeds for later production and the correctness of experimental research are guaranteed. In addition, the method can obtain the amphoteric asparagus with stable hereditary characteristics, and has guiding significance for the hybridization technology of the amphoteric asparagus.

The present application belongs to the technical field of genetic engineering, and particularly relates to a methyltransferase gene CmCMT2 and CmDRM2 application in regulating chrysanthemum flowering. The present application constructs an RNAi silencing expression vector by isolating CmCMT2 and CmDRM2 genes in a chrysanthemum variety "Jinma", and obtains CmCMT2 -RNAi and CmDRM2 -RNAi transgenic "Jinma" plants by using a genetic transformation method, then performs phenotype observation and statistics on the transgenic plants, and explores CmCMT2 and CmDRM2 the mechanism of the genes affecting early flowering of chrysanthemum by using an RNA-seq method. The CmCMT2 and CmDRM2 genes are cloned from the chrysanthemum variety "Jinma", and spatiotemporal expression analysis shows that CmCMT2 and CmDRM2 the genes are highly expressed during the process of "Jinma" from bud development to color change; and CmCMT2 and CmDRM2 the expression level in leaves gradually decreases during the growth and development of "Jinma". The research results show that CmCMT2 and CmDRM2 the content of GA1 is changed by negatively regulating the expression of CmG20ox2a and CmG20ox2b , so that the flowering period of chrysanthemum is advanced by 15 and 8 days, respectively.

The invention belongs to the field of tissue culture, and particularly relates to a saussurea longifolia callus induction and culture method which comprises the following steps: A, taking tender shoots and young leaves of wild saussurea longifolia, washing and disinfecting to obtain base tissues; b, cutting the base tissue prepared in the step A, inoculating the cut base tissue into a callus induction culture medium, and culturing to obtain an initial callus; c, carrying out subculture on the calluses prepared in the step B to obtain subcultured calluses; the tender shoots and the young leaves of the saussurea longifolia are induced to form the calluses, so that the method has the characteristics of safety, no toxicity, short period, high propagation speed, simplicity in operation and low cost, and convenience is provided for application of the saussurea longifolia in actual products.

The pollination tool comprises a supporting assembly, a vibration motor, a pollen bottle and a pollen net, the supporting assembly is connected with the pollen bottle and used for supporting the pollen bottle so that the pollen bottle can extend to the position above a pistil, the pollen bottle is provided with a containing cavity and an opening communicated with the containing cavity, and the vibration motor is arranged on the pollen bottle. The pollen can be placed in the containing cavity from the opening, the pollen net is arranged on the pollen bottle in a sleeving mode, the vibration motor is installed on the pollen bottle and used for vibrating the pollen bottle so that the pollen can fall onto stamens and buds from the through holes of the pollen net, the pollen in the pollen bottle can be scattered onto the stamens and buds through the vibration motor, the pollen pollination rate is increased, and the fruit yield is increased.

The invention belongs to the technical field of gene engineering, and particularly relates to application of methyltransferase genes CmCMT2 and CmDRM2 in regulation and control of flowering of chrysanthemum. According to the invention, CmCMT2 and CmDRM2 genes in a chrysanthemum variety 'Shenma' are separated, an RNAi silent expression vector is constructed, CmCMT2-RNAi and CmDRM2-RNAi transgenic 'Shenma' plants are obtained by using a genetic transformation method, then phenotype observation and statistics are carried out on the transgenic plants, key genes influencing chrysanthemum flowering are excavated by using an RNA-seq method, and a mechanism of the CmCMT2 and CmDRM2 genes influencing chrysanthemum early flowering is explored. The CmCMT2 gene and the CmDRM2 gene are cloned from a chrysanthemum variety 'Shenma', and spatio-temporal expression analysis shows that the CmCMT2 gene and the CmDRM2 gene are highly expressed in the process from bud development to coloring of the 'Shenma'; moreover, in the growth and development process of the'miraculous horse ', the expression levels of the CmCMT2 and the CmDRM2 in leaves are gradually reduced. Research results show that the CmCMT2 and the CmDRM2 change the content of the GA1 by negatively regulating the expression of the CmG20ox2a and the CmG20ox2b, so that the florescence of the chrysanthemum is respectively advanced by 15 days and 8 days.

The invention relates to the technical field of plant planting, in particular to a method for promoting flowering and fruit setting of amomum tsao-ko and application thereof.In the vigorous vegetative growth period, a nutrient solution composed of ammonium sulfate, NAA and GA3 with the proper concentration is adopted, the vigorous vegetative growth period can be effectively prolonged, and therefore flower bud differentiation is inhibited; a nutrient solution B composed of monopotassium phosphate, ABA and BR is sprayed in April, vegetative growth can be effectively broken through to be converted into reproductive growth, flower buds are promoted to be rapidly differentiated and formed when a rainy season comes, the flowering rate can be increased by 28-33%, the yield of the amomum tsao-ko medicinal material can be greatly increased, bumblebees are quantitatively put in the early stage of May, and the pollination rate can be greatly increased; in the blooming period, the nutrient D is spread around the cluster buds, meanwhile, the nutrient solution E is sprayed in a matched mode, and the fruit setting rate can be increased by 8.9%-9.6% compared with the fruit setting rate.

The present invention belongs to the field of tea tree plucking, and specifically relates to a device for automatically identifying and counting tea tree buds, comprising a movable guide rail and an electric slider I, each movable guide rail being slidably connected to at least two electric sliders I. The present invention achieves this by inserting a lever and a baffle between tea trees, enabling the lever and the baffle to pry the leaves and buds on the upper portion of the tea tree. The lever then moves the leaves and buds on the upper portion of the tea tree, thereby preliminarily removing water droplets adhering to the leaves and buds on the upper portion of the tea tree. The baffle then moves again to pry the leaves and buds on the upper portion of the tea tree. The movement of the baffle is blocked by the leaves and buds on the upper portion of the tea tree, causing the baffle to bend. Simultaneously, the leaves and buds on the upper portion of the tea tree are also pushed by the baffle, causing them to bend. When the baffle gradually separates from the leaves and buds on the upper portion of the tea tree, the tea tree rebounds, thereby further removing water droplets adhering to the leaves and buds on the upper portion of the tea tree.

The application discloses a kind of non-nuclear litchi and particle fruit efficient production cultivation regulation methods, mainly related to the field of tropical fruit tree cultivation physiology regulation. Including the following steps: S1.Y type directional shaping of flower spike: at the beginning of flower bud differentiation, all lateral small flower branches and top weak flower branches are removed, forming Y type flower spike;S2. Precise spraying of plant growth regulator: 15% paclobutrazol wettable powder or 10% uniconazole suspension agent is used to uniformly spray the flower spike;S3. Dynamic grading thinning: in the first thinning, only the pair of particle young fruits with uniform development are reserved;In the second fruit setting, only 3-4 pairs of particle fruits are reserved for each Y type flower spike. The beneficial effects of the application are that the directional cultivation of non-nuclear litchi and particle fruit is realized by combining flower spike directional shaping, precise flower and fruit development of plant growth regulator, supporting nutrient synergistic supply and dynamic grading thinning, which effectively improves the proportion of particle fruit, and is suitable for A4 non-nuclear litchi, south island non-nuclear litchi and other litchi varieties.

The application discloses a submerged Cinnamomum micranthum in-vitro rapid propagation and brown-preventing seedling raising method based on zygote embryos, directly induces adventitious buds of the submerged Cinnamomum micranthum for the first time, significantly improves a propagation coefficient, and overcomes problems such as vitrification and browning of the adventitious buds of the submerged Cinnamomum micranthum. The application establishes an efficient and stable in-vitro regeneration system of the submerged Cinnamomum micranthum, and provides a basis for large-scale seedling raising of the submerged Cinnamomum micranthum.

The invention provides a regulator for promoting bougainvillea speetabilis to bloom or inhibiting buds from falling off, and relates to the technical field of plant regulators. The regulator provided by the invention is selected from one or more of 0.1 to 0.3 mg / L of fluid boron, 1 to 3 mg / L of KH2PO4, 5 to 25 mg / L of NAA and 50 mg / L of sugar alcohol calcium. According to the invention, fluid boron, KH2PO4, NAA and sugar alcohol calcium are selected and compounded, and under natural production and cultivation conditions and dark conditions of simulated storage and transportation, comprehensive and efficient regulation and control schemes for regulating and controlling the period of the bougainvillea speetabilis and inhibiting shedding are respectively screened out; the regulator is applied through exogenous spraying, flowering of the bougainvillea speetabilis can be well promoted or bud falling is inhibited, the ornamental value and storage and transportation resistance of the bougainvillea speetabilis are improved, and a basis is provided for production, cultivation, management and transportation in practical application of the bougainvillea speetabilis.

The present application provides a kind of for promoting bougainvillea spectabilis flowering or inhibiting bud drop regulator, it is related to plant regulator technical field.The regulator of the present application is selected from one or more of 0.1-0.3mg / L fluid boron, 1-3mg / L KH2PO4, 5-25mg / L NAA, 50mg / L calcium sugar alcohol.The present application is selected by fluid boron, KH2PO4, NAA, sugar alcohol calcium, compounded, under the natural production cultivation condition and the dark condition of simulated storage and transportation, respectively, screen out the comprehensive efficient regulation bougainvillea spectabilis flowering, inhibit the regulation scheme of drop;By exogenous spraying to apply regulator, can well promote bougainvillea spectabilis flowering or inhibit bud drop, improve the ornamental and storage and transportation of bougainvillea spectabilis, provide basis for the production cultivation management and transportation in the practical application of bougainvillea spectabilis.

The present invention discloses a method for cultivating a florid Paphiopedilum callosum. The method comprises the following steps: selecting plants with 5-6 leaves, strong growth, and a well-developed root system for root irrigation with a 6-BA solution at a concentration of 200 to 800 mg / L, treating them once every two weeks for a total of 5-6 times, and performing unique fertilizer and water management during the subsequent cultivation process. The present invention utilizes a 6-BA solution to root-irrigate seedlings transplanted for one year to significantly increase the number of tillering buds, and performs appropriate fertilizer and water management during the subsequent cultivation process, which can cause most of the tillering lateral buds and main buds to bloom together, significantly increasing the number of flowers. The method can promote a single Paphiopedilum callosum plant to bloom multiple branches, improve its ornamental value, fill domestic and international gaps, and have a positive driving effect on promoting the development of my country's Paphiopedilum industry.

The utility model relates to the technical field of amomum tsao-ko bud and bud protection, and discloses an amomum tsao-ko bud and bud protection device which comprises soil, an amomum tsao-ko bud and an amomum tsao-ko trunk, the top of the soil is fixedly connected with the amomum tsao-ko bud, and the position, corresponding to the top of the soil, of the outer side of the amomum tsao-ko bud is movably connected with the amomum tsao-ko trunk. The top of an amomum tsao-ko trunk is fixedly connected with the top of soil, and a large amount of rainwater directly falling on amomum tsao-ko flower buds can be effectively blocked and dispersed by designing the inclined rain baffle, so that physical damage and disease infection possibly caused by rain wash to young and tender flower buds are avoided, normal growth and development of amomum tsao-ko are ensured, and the survival rate of the amomum tsao-ko is increased. The rain shielding plate not only plays a role in shielding rain in rainy days, but also can provide proper sun shading for the amomum tsao-ko flower buds in sunny days, so that the influence of high temperature caused by direct sunlight is reduced, the local microclimate is favorably adjusted, and the amomum tsao-ko is protected from heat damage.

The application provides a hard branch cutting breeding method of Actinidia kolomikta and Zizyphus jujuba, relates to the technical field of asexual reproduction and seedling raising, and comprises the following steps: (1) collecting 1-2 year old branches and pruning branch tips; (2) cutting into 10cm cuttings with more than two bud points, with the upper end of the cutting being inclined and the lower end being horizontal, and soaking the cutting in carbendazim for sterilization treatment; (3) selecting grass charcoal mixed with perlite as a cutting substrate, disinfecting the substrate after being loaded into a plug tray; (4) preparing a rooting agent, treating the cutting of step (2), inserting the cutting into the substrate of the plug tray, fixing the cutting, pouring carbendazim through the substrate, and covering the cutting with a transparent cover; (5) placing the cutting under the condition of 70% shading, increasing 20% illumination after new leaves are unfolded, controlling the temperature to be 10-30 DEG C, timely watering the cutting after cutting, combining with carbendazim sterilization, gradually opening the transparent cover after 40d of cutting, watering according to the situation, and spraying foliar fertilizer every 10d; and (6) transplanting and planting the cutting after 60d of cutting. The application has the beneficial effects of simplifying the hard branch cutting steps of Actinidia kolomikta and Zizyphus jujuba and shortening the cutting breeding period.

The application belongs to the field of genetic engineering, and particularly relates to application of an ELF3 gene in regulating growth of a poplar top bud. Through screening and testing, it is found that knocking out the ELF3 gene can achieve the technical effect of delaying growth stop and dormancy time of a tree top bud under short-day conditions and promoting tree growth.