180 results about "Bud" patented technology

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

The present application belongs to the technical field of genetic engineering, and particularly relates to a methyltransferase gene CmCMT2 and CmDRM2 application in regulating chrysanthemum flowering. The present application constructs an RNAi silencing expression vector by isolating CmCMT2 and CmDRM2 genes in a chrysanthemum variety "Jinma", and obtains CmCMT2 -RNAi and CmDRM2 -RNAi transgenic "Jinma" plants by using a genetic transformation method, then performs phenotype observation and statistics on the transgenic plants, and explores CmCMT2 and CmDRM2 the mechanism of the genes affecting early flowering of chrysanthemum by using an RNA-seq method. The CmCMT2 and CmDRM2 genes are cloned from the chrysanthemum variety "Jinma", and spatiotemporal expression analysis shows that CmCMT2 and CmDRM2 the genes are highly expressed during the process of "Jinma" from bud development to color change; and CmCMT2 and CmDRM2 the expression level in leaves gradually decreases during the growth and development of "Jinma". The research results show that CmCMT2 and CmDRM2 the content of GA1 is changed by negatively regulating the expression of CmG20ox2a and CmG20ox2b , so that the flowering period of chrysanthemum is advanced by 15 and 8 days, respectively.

The application discloses a submerged Cinnamomum micranthum in-vitro rapid propagation and brown-preventing seedling raising method based on zygote embryos, directly induces adventitious buds of the submerged Cinnamomum micranthum for the first time, significantly improves a propagation coefficient, and overcomes problems such as vitrification and browning of the adventitious buds of the submerged Cinnamomum micranthum. The application establishes an efficient and stable in-vitro regeneration system of the submerged Cinnamomum micranthum, and provides a basis for large-scale seedling raising of the submerged Cinnamomum micranthum.

The invention provides a regulator for promoting bougainvillea speetabilis to bloom or inhibiting buds from falling off, and relates to the technical field of plant regulators. The regulator provided by the invention is selected from one or more of 0.1 to 0.3 mg / L of fluid boron, 1 to 3 mg / L of KH2PO4, 5 to 25 mg / L of NAA and 50 mg / L of sugar alcohol calcium. According to the invention, fluid boron, KH2PO4, NAA and sugar alcohol calcium are selected and compounded, and under natural production and cultivation conditions and dark conditions of simulated storage and transportation, comprehensive and efficient regulation and control schemes for regulating and controlling the period of the bougainvillea speetabilis and inhibiting shedding are respectively screened out; the regulator is applied through exogenous spraying, flowering of the bougainvillea speetabilis can be well promoted or bud falling is inhibited, the ornamental value and storage and transportation resistance of the bougainvillea speetabilis are improved, and a basis is provided for production, cultivation, management and transportation in practical application of the bougainvillea speetabilis.

The present application provides a kind of for promoting bougainvillea spectabilis flowering or inhibiting bud drop regulator, it is related to plant regulator technical field.The regulator of the present application is selected from one or more of 0.1-0.3mg / L fluid boron, 1-3mg / L KH2PO4, 5-25mg / L NAA, 50mg / L calcium sugar alcohol.The present application is selected by fluid boron, KH2PO4, NAA, sugar alcohol calcium, compounded, under the natural production cultivation condition and the dark condition of simulated storage and transportation, respectively, screen out the comprehensive efficient regulation bougainvillea spectabilis flowering, inhibit the regulation scheme of drop;By exogenous spraying to apply regulator, can well promote bougainvillea spectabilis flowering or inhibit bud drop, improve the ornamental and storage and transportation of bougainvillea spectabilis, provide basis for the production cultivation management and transportation in the practical application of bougainvillea spectabilis.

The application provides a hard branch cutting breeding method of Actinidia kolomikta and Zizyphus jujuba, relates to the technical field of asexual reproduction and seedling raising, and comprises the following steps: (1) collecting 1-2 year old branches and pruning branch tips; (2) cutting into 10cm cuttings with more than two bud points, with the upper end of the cutting being inclined and the lower end being horizontal, and soaking the cutting in carbendazim for sterilization treatment; (3) selecting grass charcoal mixed with perlite as a cutting substrate, disinfecting the substrate after being loaded into a plug tray; (4) preparing a rooting agent, treating the cutting of step (2), inserting the cutting into the substrate of the plug tray, fixing the cutting, pouring carbendazim through the substrate, and covering the cutting with a transparent cover; (5) placing the cutting under the condition of 70% shading, increasing 20% illumination after new leaves are unfolded, controlling the temperature to be 10-30 DEG C, timely watering the cutting after cutting, combining with carbendazim sterilization, gradually opening the transparent cover after 40d of cutting, watering according to the situation, and spraying foliar fertilizer every 10d; and (6) transplanting and planting the cutting after 60d of cutting. The application has the beneficial effects of simplifying the hard branch cutting steps of Actinidia kolomikta and Zizyphus jujuba and shortening the cutting breeding period.

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The invention discloses a thrip resistance identification device and application. The device comprises an inoculation barrel, a preservation substrate is laid at the bottom in the inoculation barrel, an isolation structure with one or more through holes covers the preservation substrate, and a sealing structure with one or more air holes covers an opening in the top of the inoculation barrel. When the device is used, the whole plant does not need to be cultivated, in-vitro flowering branches can be picked for identification, rapid screening of large-scale germplasm resources can be achieved, and repeatability and consistency of experimental results are good. Meanwhile, when the device is used, buds and functional leaves of in-vitro flowering branches are reserved, the actual feeding part of the Frankliniella occidentalis is simulated, and compared with in-vitro leaf inoculation, the real resistance level can be better reflected. The device has the advantages of easily available materials, low cost and strong operability, can be used for identifying the resistance of the Frankliniella occidentalis of various materials such as chrysanthemum and related wild species, cultivated species, breeding offspring and the like, provides a high-quality parent material for insect-resistant breeding, can be used for researching an insect-resistant mechanism, and is suitable for popularization and application in basic breeding units.

The application discloses a tissue culture propagation method of eriochloa contracta. The creeping stem section of the eriochloa contracta is collected, washed, and then sterilized and dried; the sterilized and treated stem section is inoculated into a bud induction medium of MS+1-1.5 mg / L 6-BA+0.1-0.5 mg / L NAA+30 mg / L sucrose+6.5 mg / L agar to perform bud induction, and after 15-20 days of culture, the adventitious bud seedling is obtained; the adventitious bud of about 1-1.5 cm induced in the primary culture medium is inoculated into a proliferation culture medium to perform subculture, and the next step can be divided into single bud expansion or direct transplanting domestication. The two-step method only needs primary culture-proliferation and rooting to be simultaneously performed, and the transplanting survival rate is high.

The invention discloses an efficient quality-guaranteeing processing method for lilies. The efficient quality-guaranteeing processing method comprises the following steps of harvesting and sorting, staged drying, gradient drying, homogenizing and moisture regaining, packaging, final sorting after moisture regaining, elimination of individuals which are not completely dried, and then sealed packaging. According to the efficient quality-guaranteeing processing method for the lilies, firstly, through strict harvesting standards and field sorting, the uniformity of the quality of the raw materials is guaranteed from the source, and a good foundation is laid for subsequent processing; and secondly, a strategy of treating flowers and flower buds in stages is adopted, and a gradient drying process with multi-stage precise control is combined, so that efficient and mild dehydration can be realized according to the characteristics of different materials, and the natural color, form and effective components of the lilies are reserved to the greatest extent. And finally, through a unique homogenizing and moisture regaining process, the internal moisture of the finished flowers is effectively balanced, the problem of local mildew or fragility is avoided, and the stability, the storage safety and the commodity appearance of the product are remarkably improved.

This invention relates to a rapid induction culture medium and method for inducing bud formation from kiwifruit leaves to complete plants, belonging to the field of biotechnology. The callus induction culture medium for rapidly inducing bud formation from kiwifruit leaves includes MS medium, sucrose, 6-benzylaminopurine, naphthaleneacetic acid, thidiazuron, and agar, with the following weight ratios: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.000001%-0.000003%, naphthaleneacetic acid 0.0000002%-0.0000008%, thidiazuron 0.000001%-0.000003%, agar 0.006%-0.01%, and the balance being water. The formula for the callus induction bud culture medium is as follows: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.0000005%-0.000003%, naphthaleneacetic acid 0.00000005%-0.0000002%, agar 0.006%-0.01%, with the balance being water. This invention provides a rapid induction culture medium and method for kiwifruit leaves to complete plants, effectively inhibiting problems such as callus waterlogging, browning, and vitrification. It not only increases the number of buds but also improves the quality of buds, which is beneficial to the growth and differentiation of callus tissue, making it easier for it to differentiate into buds, thus laying a solid foundation for subsequent genetic transformation experiments.

The application discloses a method for high-frequency regeneration of Rhododendron chrysanthum based on cotyledon node indirect organogenesis, and belongs to the field of plant biotechnology. The method takes the cotyledon node of Rhododendron chrysanthum sterile seedling as an explant, and obtains a complete regenerated plant through the induction of callus, differentiation of adventitious buds, elongation of adventitious buds, strong seedling and rooting culture in sequence. The application realizes the super-high-frequency differentiation of adventitious buds by combining the cotyledon node explant with a specific hormone combination for the first time, the differentiation coefficient is as high as 96, and the rooting rate is 92.7%, thereby effectively solving the technical bottleneck of low propagation efficiency of Rhododendron chrysanthum, and providing reliable technical support for the conservation and industrialization development of Rhododendron chrysanthum germplasm resources.

A method for rapidly restoring Phragmites australis population in degraded land through stolon domestication is provided, including the following steps: at the beginning of the Phragmites australis growing season from May to June every year, the Phragmites australis is made to crawl by draining water and artificially crushing the aboveground stems, and then the sprouting of stolon internode buds or root buds is promoted by continuously controlling water; in mid-July, suitable stolons containing internode buds or root buds are selected, and the root segments are dug from the base, and the stolons are wound in a circle in the extension direction and fixed, and sprayed with water to keep moisture; the fixed stolons are unfolded and transplanted into degraded land for daily water and fertilizer management.

The present application belongs to the field of plant tissue culture and genetic transformation technology, and particularly relates to a method for obtaining high-frequency embryogenic material of plants. The method comprises the following steps: inoculating plant explants on a callus induction medium for culture; continuously subculturing the callus for 4 to 6 times; transferring the embryogenic callus grown after subculture to a sprouting medium for culture, and inducing the embryogenic callus to form adventitious buds on the sprouting medium; transferring the adventitious buds to a rooting medium for culture until roots are formed; transplanting the plants with roots, and culturing until maturity and fruiting to obtain the high-frequency embryogenic material. The method provided by the present application can inoculate mature embryos of low-frequency embryogenic material on an induction medium to induce the formation of embryogenic callus, and then regenerate and induce the embryogenic callus to form seedlings, and through one or more repeated screening, the high-frequency embryogenic material under the genotype background can be obtained.

The invention provides a corydalis pygmaea terminal bud organogenesis and in-vitro rapid propagation method, and belongs to the technical field of plant tissue culture. Comprising the following steps: by taking a terminal bud of a corydalis pygmaea aseptic seedling as an explant, sequentially performing adventitious bud differentiation culture, cluster bud multiplication culture and rooting induction to obtain a corydalis pygmaea tissue culture seedling, pretreating a root system of the corydalis pygmaea tissue culture seedling with nano-zinc oxide, and transplanting the corydalis pygmaea tissue culture seedling. According to the method, the terminal bud is used as the explant, the organ occurrence rate and the propagation coefficient are remarkably increased by optimizing the culture medium formula and the culture conditions, the induction rate reaches 85% or above, the browning rate is controlled to be 10% or below, the proliferation multiple per four weeks can reach 11-14 times, the rooting rate exceeds 90%, rapid and efficient propagation of corydalis pygmaea is achieved, reliable technical support is provided for large-scale production, and the method has a wide application prospect. The problem of raw material supply can be effectively solved, and a foundation is laid for industrial production of active ingredients.

The invention discloses a method for improving the transplanting survival rate of taiwania flousiana container seedlings, and relates to the technical field of seedling cultivation, and the method comprises the following steps: selecting a plot with smooth gradient and good ventilation and illumination, and preparing sterile deep nutrient soil with proper sand grain content; selecting taiwania flousiana seedlings with plump terminal buds and healthy root systems, and performing sterilization and insect killing treatment on the land parcels after ladder opening and soil preparation; nutrient soil is bagged and placed normatively, a shed frame space is reserved, and an elevated arched shed is built and covered with a shed film and a high-sunshade-degree sunshade net; the nutrition bags are watered for the first time until the humidity reaches the standard, nursery stocks are planted after being watered before being lifted, moisturized and transported and subjected to field root pruning, root fixing water is watered at nightfall, and then a greenhouse opening is closed; the method solves the problems of easy damage of root systems, lack of environmental regulation and the like in traditional transplantation, reduces the activity loss of nursery stocks and the risk of plant diseases and insect pests, and significantly improves the transplantation survival rate.

A new Lantana plant particularly distinguished by having an excellent branching and mounding habit, a flower color that changes during flower development from orange buds to yellow-orange when the flower opens, and to bright magenta as the final color, and large flowers with continuous flowering from April through November, is disclosed.

The application discloses a kind of new afforestation land of Lingnan region rhizoma zingiberis and ganoderma lucidum imitated wild three-dimensional planting method, which comprises the following steps: 1) selecting the new afforestation land of Lingnan region planted with trees within 2 years;2) ridging between the tree rows of new afforestation land, digging ganoderma lucidum planting ditch on the ridge, placing ganoderma lucidum stick horizontally in the ganoderma lucidum planting ditch, connecting head to tail between sticks, covering with broken soil, covering soil layer on the stick, covering dry grass on the soil layer, and carrying out ganoderma lucidum imitated wild planting;3) in April-May, digging hole between ganoderma lucidum sticks, putting one section of rhizome of zingiber officinale rose into each hole after mixing with organic fertilizer and soil, putting bud head upward, filling fine topsoil while putting, and carrying out zingiber officinale imitated wild planting;4) regional weeding and zingiber officinale fertilization;5) ganoderma lucidum and zingiber officinale harvesting.The method can not only realize ganoderma lucidum and zingiber officinale imitated wild planting in new afforestation land, but also improve the utilization rate of new afforestation land, achieve the purpose of cultivation instead of rearing and short-term cultivation instead of long-term cultivation, realize three-dimensional planting of new afforestation land, and improve economic benefit.

The invention discloses a method for efficiently inducing adventitious buds and rooting through transgenic hairy roots of Populus albus Nanlin 895, and relates to the technical field of biological breeding. The plant material used in the invention is a transgenic Nanlin 895 poplar hairy root generated by mediation of agrobacterium rhizogenes; dividing the hairy roots into root sections of 1-2 cm by using a sterile blade, and putting the hairy roots on a culture medium added with 0.01 mg / L TDZ and 0.2 mg / L 6-BA to induce adventitious buds; placing the adventitious buds in a culture medium added with 0.01 mg / L of TDZ for bud elongation; and cutting off the elongated adventitious buds, placing the adventitious buds in a culture medium added with 0.1 mg / L IBA, and carrying out rooting culture so as to obtain a positive Nanlin 895 poplar transgenic plant. Experiments prove that the adventitious bud induction rate of the plant material is 70%, the adventitious bud elongation rate is 40%, the rooting rate is 90%, and the method has good practicability.

The invention discloses a method for propagating robust artificial seed stems of pinellia ternate through low-cost tissue culture. The method comprises the steps of germination, explant disinfection, establishment of a primary culture system, petiole subculture propagation, tuber subculture propagation and artificial seed stem harvesting. The plant regeneration approach designed by the invention is to induce one end of the petiole to directly generate a small tuber with a complete form under hormone-free concentration, and the small tuber is very similar to the part and the form of the bulbils generated under natural conditions. Tubers are not subjected to a callus stage, are uniform in size and high in hereditary stability, can maintain the characteristics of excellent varieties, and are an optimal way for in-vitro rapid propagation of pinellia ternate. A large number of mature, full, neat and consistent third-generation tubers with high germination rate, stress resistance and storage resistance can be obtained in a short time, the tubers can be directly transplanted to a field without special domestication, the survival rate is high, practical operation and large-scale production are facilitated, and production is not limited by seasons.

The invention relates to a method for regulating and controlling a flower type by silencing a peony organ gene based on a transient expression system, which belongs to a peony flower type regulation and control method and comprises the following steps of: designing a specific primer aiming at a target gene PsuAG, obtaining a target fragment through PCR (Polymerase Chain Reaction) amplification, and inserting the target fragment into a transient expression vector to construct a gene silencing fusion expression vector; introducing the gene silencing fusion expression vector into an agrobacterium competent cell, preparing an infection solution to infect a peony plant, and injecting the infection solution into a pedicel by using an injector when a flower bud of the peony plant develops to an initial stage of a large wind chime period; when the flower buds grow to the initial stage of the round peach stage, injecting the infection liquid into pedicels and sepal bases; after each time of injection, weak light culture is carried out under a shading condition. The efficient silencing of the key gene PsuAG for regulating stamen petals is realized, and the double petals rate of the peony flower type is improved. The method does not need to depend on a stable genetic transformation system, operation is directly carried out on floral organs of a complete plant, and the gene function verification period is remarkably shortened.

The invention belongs to the technical field of bud promoting and seedling raising, and discloses a Chinese orchid bud promoting and seedling raising method, which is characterized in that three radial cutting lines forming 120-degree included angles with one another are manufactured along the longitudinal axis direction of a pseudobulb by cutting from the base of an epiphytic leaf at the top end of the pseudobulb of an orchid seedling. The cutting depth is 30% of the radial thickness of the pseudobulb (1t; 1 / 3 of the radius of the pseudobulb). While the pseudobulb is effectively stimulated to generate calluses and germinate new buds, the original vascular bundle system, leaf buds and root bases of the pseudobulb are protected from structural damage to the greatest extent. The incision can absorb hormone more easily, and the germination rate is increased. The orchid plants subjected to hormone treatment are comprehensively sprayed and disinfected with 600-fold 75% chlorothalonil liquid for three times, spraying each time needs to be carried out after the last time of liquid medicine is completely dried, it is ensured that pseudobulbs and incisions are covered in place, the situation of germ infection at the incisions is prevented, and the survival rate is increased.

This utility model relates to the field of cultivation auxiliary technology, specifically to a cultivation container for edible fungi, including a cultivation box. The cultivation box has an inner cavity, and a handle is fixed to its outer wall. A limiting partition is inserted and installed inside the cultivation box, and a bud-sparser board is installed above the limiting partition, covering the upper part of the inner cavity. A base is installed at the bottom of the cultivation box, and an upper upright plate is vertically installed on the side of the top of the cultivation box, with an outer cover covering the upper upright plate. This utility model facilitates assembly and maintenance according to usage needs, thus improving operational efficiency.

The invention discloses a Rhododendron mucronatum callus induction in-vitro regeneration method. The method comprises the following steps: selecting a stem segment of a sterile sowing seedling as an explant; inoculating the explant into a callus induction culture medium, culturing for 30 days under a dark condition, and then culturing under an illumination condition; transferring the calluses into a differential culture medium, continuously culturing under an illumination condition, and inducing the adventitious buds to differentiate, so as to obtain regenerated plants. According to the method, through explant design, in combination with a callus induction culture medium and a differentiation culture medium of a specific formula and accurately controlled culture conditions, the induction rate of the rhododendron erythrophyllum callus reaches 96.67%, the browning rate is only 4%, the differentiation rate of the adventitious buds reaches 71.11%, more than 63 adventitious buds can be differentiated from each callus block on average, the rooting rate reaches 63.33%, and the survival rate of the rhododendron erythrophyllum callus is greatly improved. Reliable technical support is provided for large-scale production and genetic improvement of Rhododendron mucronatum.

The invention discloses a polygonatum cyrtonema high-yield cultivation method based on dynamic ridge expansion and ecological shading, and belongs to the technical field of traditional Chinese medicinal material cultivation. The method comprises the following steps: selecting high-quality seed stems; the seedlings are placed in ridges in a high-density single-row mode, and bud heads face the two sides alternately during placement; sowing high-stem crops at ridge edges in the early growth stage and in furrows in the later growth stage so as to ecologically shade and improve the soil structure; fine weeding is carried out by adopting a partitioning strategy; and a dynamic management mode of'year-by-year ridge expansion and fertilization 'is adopted. Compared with a traditional fixed ridge mode, the yield per mu can be remarkably increased by 27% or above, the occurrence rate of plant diseases and insect pests is reduced, meanwhile, the weeding labor cost is greatly reduced, and the soil structure and fertility are effectively improved. According to the method, the biological characteristics of the polygonatum cyrtonema are accurately matched, the problems of low utilization rate of fixed ridge cultivation organic fertilizer, high weeding cost, later soil hardening, serious root rot and high shading cost are effectively solved, the yield and commodity quality of the polygonatum cyrtonema are remarkably improved, and the method is suitable for large-scale popularization and application.

The invention relates to the technical field of intelligent monitoring of plant maturity, and discloses an intelligent monitoring method and system for the maturity of cauliflower flower buds, and the method comprises the steps: analyzing the flower bud distribution density according to a pre-obtained cauliflower image, carrying out the multi-angle image collection of an area with the flower bud distribution density greater than a preset density threshold value, and obtaining a first image set; extracting morphological parameters of the flower buds, identifying flower bud contours, and calculating flower bud volumes; extracting morphological characteristics of an area in the flower bud contour, and calculating a probability value of the flower bud belonging to a preset maturity level through characteristic fusion analysis in combination with the flower bud volume to obtain the maturity of the flower bud; in combination with the maturity of the flower buds and historical growth data, the growth process of the flower buds is simulated, the maturity time of the flower buds is predicted, and the maturity of the flower buds of the cauliflower is intelligently monitored; according to the method, the maturity of the flower buds of the cauliflower is intelligently monitored, so that the prediction accuracy of the flower bud maturity time can be improved, and accurate harvesting, yield increase and efficiency increase of the cauliflower are realized.

The application discloses a method for establishing an in-vitro regeneration system of overwintering buds of forest wild ginseng, and comprises the following steps: 1, the best disinfection method of the overwintering buds is 75% alcohol for 60s and 0.1% exogenous tissue disinfectant for 7min, and the overwintering buds are cultured into aseptic seedlings; 2, callus induction is carried out on stems, leaves and flowers of the aseptic seedlings, and the induction rate is counted; the induction rate of the flower buds is higher in LH culture medium containing 1mg / L 2,4-D hormone, and the induction rate of the stems and leaves is higher in LH culture medium containing 0.5mg / L KT+2mg / L 2,4-D hormone; 3, the callus of each part of the aseptic seedling stems, leaves and flowers is cultured into adventitious roots, embryoid induction and regenerated seedlings, and the best induction part is screened; the flower buds are used as the exogenous tissue, and the differentiation rate of the adventitious roots, the induction rate of the embryoid and the seedling rate are significantly higher than those of other parts; 4, the ploidy of the regenerated seedling leaves is identified, and it is found that the regenerated seedling leaves are tetraploid and do not have ploidy mutation. The application has the advantages that an efficient regeneration technology system of forest wild ginseng is established, and theoretical and practical bases are provided for rapid breeding and germplasm resource protection of the forest wild ginseng.

The application belongs to the field of genetic engineering, and particularly relates to application of a LUX gene in regulating growth of a poplar top bud. Through screening and testing, it is found that knocking out the LUX gene can achieve the technical effects of delaying growth stop and dormancy time of a tree top bud under short-day conditions and promoting growth of the tree.

The invention belongs to the technical field of agricultural biology, and particularly discloses a grape germplasm innovation method based on X-ray gradient mutagenesis and an LD50 precise prediction model, and the method comprises the following steps: selecting an annual grape branch for vernalization treatment, and trimming the annual grape branch into a single-bud stem segment; setting an irradiation time gradient, accurately calculating an LD50 value through a cubic spline interpolation model, and carrying out X-ray irradiation on the single-bud stem segment according to the LD50 value; after irradiation, performing cutting and liquid paraffin sealing, and then performing cuttage, potting and field planting treatment; performing secondary vernalization on mutagenesis plant branches, trimming the mutagenesis plant branches into single-bud stem segments, collecting tender leaves after cutting survival, extracting genome DNA, performing whole genome sequencing, and accurately distinguishing mutagenesis specific variation and natural variation. By establishing a complete technical system of standardized vernalization, gradient irradiation, LD50 accurate calculation and whole genome verification, the problems of inaccurate irradiation parameters, nonstandard material treatment, low mutation screening accuracy and the like in the prior art are solved.

The invention discloses a white flower ornithogalum caudatum OtEXO gene, an encoding protein and application, and belongs to the technical field of molecular biology. The nucleotide sequence of the ornithogalum caudatum OtEXO gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the ornithogalum caudatum OtEXO gene is as shown in SEQ ID NO. 2. The ornithogalum caudatum OtEXO gene can be applied to regulation and control of plant development, and overexpression of the OtEXO gene promotes leaf enlargement, accelerates plant growth and development and promotes growth of bulbils on leaves. The ornithogalum caudatum OtEXO gene disclosed by the invention provides a new gene for cultivating a new variety with enlarged leaves and other organs, excellent plant growth vigor and accelerated breeding speed, and can be widely applied to the fields of plant organ development and molecular breeding.