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285 results about "Bud" patented technology

In botany, a bud is an undeveloped or embryonic shoot and normally occurs in the axil of a leaf or at the tip of a stem. Once formed, a bud may remain for some time in a dormant condition, or it may form a shoot immediately. Buds may be specialized to develop flowers or short shoots, or may have the potential for general shoot development. The term bud is also used in zoology, where it refers to an outgrowth from the body which can develop into a new individual.

High-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings

InactiveCN105660397AContinuation of good traitsFast propagation cycle shortenedGrowth substratesCulture mediaAxillary budBud
The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.
Owner:HANGZHOU LANDSCAPING

Application and method of knockout poplar LUX1 gene in maintaining bud growth and wood development under short day

PendingCN120272491APlant peptidesFermentationAmino acid sequence alignmentBud growth
The invention discloses an application and a method for knocking out a poplar LUX1 gene in delaying growth stop or dormancy time under short day, and the poplar LUX1 gene is identified and obtained through amino acid sequence comparison with arabidopsis thaliana LUX. The poplar LUX1 gene is a protein for coding an amino acid sequence as shown in SEQ ID No.1. The LUX1 gene is knocked out through a CRISPR / Cas9 editing system, and the time that the poplar enters the dormancy period due to short sunlight in autumn is delayed, so that the poplar germplasm with long growth period and high biomass is obtained. After the LUX1 gene is knocked out, terminal bud growth stop and stem cambium activity reduction in the seasonal growth process of the poplar can be delayed, so that wood development is promoted. Deferring of the dormancy time under the autumn short-day condition has a great application prospect for prolonging the growth period of the trees so as to improve photosynthetic carbon sequestration and biomass accumulation.
Owner:SOUTHWEST UNIV

Citrus sapling flower killing agent as well as preparation method and application thereof

The invention relates to the technical field of agricultural chemistry, and discloses a citrus sapling flower killing agent and a preparation method and application thereof, the flower killing agent comprises fluoroglycofen-ethyl, the dilution concentration of the fluoroglycofen-ethyl is 15000-25000 times, and the dosage form is 10% missible oil or 20% microemulsion. When the pesticide is used, the pesticide application time is the flower bud development S1 stage, and the spraying amount is 50-80 L / mu. According to the invention, the traditional application of fluoroglycofen-ethyl as a herbicide is broken through, the fluoroglycofen-ethyl is innovatively developed into the citrus sapling flower-killing agent, the functional boundary of pesticides is widened, and a brand new chemical regulation idea is provided for the flower control of woody plants; through fixed pesticide application period, concentration, environmental parameters and other elements, a reproducible'chemical pruning 'technical regulation is formed, the volatility of traditional empirical management is changed, and scientific and standardized development of citrus sapling management is promoted.
Owner:SOUTHWEST UNIV

Medicago sativa MsCBR1 gene as well as encoding protein and application thereof

The invention discloses a medicago sativa MsCBR1 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a medicago sativa gene MsCBR1 is found, and a medicago sativa mscbr1 mutant is constructed by knocking out the MsCBR1 gene. After cutting, compared with a control plant, the alfalfa mscbr1 mutant has the advantages that the number of regenerated branches, the number of leaves and the number of crown buds are reduced. Therefore, it is determined that the MsCBR1 gene has a regulation effect on regeneration of plants after alfalfa is cut. Therefore, the invention provides new theoretical support for cultivating multi-branch and high-yield medicago sativa materials.
Owner:INST OF BOTANY CHINESE ACAD OF SCI

Application of methyltransferase genes CmCMT2 and CmDRM2 in regulating chrysanthemum flowering

The present application belongs to the technical field of genetic engineering, and particularly relates to a methyltransferase gene CmCMT2 and CmDRM2 application in regulating chrysanthemum flowering. The present application constructs an RNAi silencing expression vector by isolating CmCMT2 and CmDRM2 genes in a chrysanthemum variety "Jinma", and obtains CmCMT2 -RNAi and CmDRM2 -RNAi transgenic "Jinma" plants by using a genetic transformation method, then performs phenotype observation and statistics on the transgenic plants, and explores CmCMT2 and CmDRM2 the mechanism of the genes affecting early flowering of chrysanthemum by using an RNA-seq method. The CmCMT2 and CmDRM2 genes are cloned from the chrysanthemum variety "Jinma", and spatiotemporal expression analysis shows that CmCMT2 and CmDRM2 the genes are highly expressed during the process of "Jinma" from bud development to color change; and CmCMT2 and CmDRM2 the expression level in leaves gradually decreases during the growth and development of "Jinma". The research results show that CmCMT2 and CmDRM2 the content of GA1 is changed by negatively regulating the expression of CmG20ox2a and CmG20ox2b , so that the flowering period of chrysanthemum is advanced by 15 and 8 days, respectively.
Owner:HENAN UNIVERSITY

Method for promoting flower and fruit setting of amomum tsao-ko and application

The invention relates to the technical field of plant planting, in particular to a method for promoting flowering and fruit setting of amomum tsao-ko and application thereof.In the vigorous vegetative growth period, a nutrient solution composed of ammonium sulfate, NAA and GA3 with the proper concentration is adopted, the vigorous vegetative growth period can be effectively prolonged, and therefore flower bud differentiation is inhibited; a nutrient solution B composed of monopotassium phosphate, ABA and BR is sprayed in April, vegetative growth can be effectively broken through to be converted into reproductive growth, flower buds are promoted to be rapidly differentiated and formed when a rainy season comes, the flowering rate can be increased by 28-33%, the yield of the amomum tsao-ko medicinal material can be greatly increased, bumblebees are quantitatively put in the early stage of May, and the pollination rate can be greatly increased; in the blooming period, the nutrient D is spread around the cluster buds, meanwhile, the nutrient solution E is sprayed in a matched mode, and the fruit setting rate can be increased by 8.9%-9.6% compared with the fruit setting rate.
Owner:INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1

A tea bud automatic identification and counting device

The present invention belongs to the field of tea tree plucking, and specifically relates to a device for automatically identifying and counting tea tree buds, comprising a movable guide rail and an electric slider I, each movable guide rail being slidably connected to at least two electric sliders I. The present invention achieves this by inserting a lever and a baffle between tea trees, enabling the lever and the baffle to pry the leaves and buds on the upper portion of the tea tree. The lever then moves the leaves and buds on the upper portion of the tea tree, thereby preliminarily removing water droplets adhering to the leaves and buds on the upper portion of the tea tree. The baffle then moves again to pry the leaves and buds on the upper portion of the tea tree. The movement of the baffle is blocked by the leaves and buds on the upper portion of the tea tree, causing the baffle to bend. Simultaneously, the leaves and buds on the upper portion of the tea tree are also pushed by the baffle, causing them to bend. When the baffle gradually separates from the leaves and buds on the upper portion of the tea tree, the tea tree rebounds, thereby further removing water droplets adhering to the leaves and buds on the upper portion of the tea tree.
Owner:RIZHAO ACAD OF AGRI SCI

A method for in-vitro rapid propagation and brown prevention of Lindera glauca based on zygotic embryos

PendingCN122162707APlant tissue cultureHorticulture methodsLindera glaucaEmbryo
The application discloses a submerged Cinnamomum micranthum in-vitro rapid propagation and brown-preventing seedling raising method based on zygote embryos, directly induces adventitious buds of the submerged Cinnamomum micranthum for the first time, significantly improves a propagation coefficient, and overcomes problems such as vitrification and browning of the adventitious buds of the submerged Cinnamomum micranthum. The application establishes an efficient and stable in-vitro regeneration system of the submerged Cinnamomum micranthum, and provides a basis for large-scale seedling raising of the submerged Cinnamomum micranthum.
Owner:JIANGXI ACAD OF FORESTRY

Regulator for promoting blooming of bougainvillea speetabilis or inhibiting bud shedding

ActiveCN121369418ABiocideFlowers cultivationPlant regulatorsAnthesis
The invention provides a regulator for promoting bougainvillea speetabilis to bloom or inhibiting buds from falling off, and relates to the technical field of plant regulators. The regulator provided by the invention is selected from one or more of 0.1 to 0.3 mg / L of fluid boron, 1 to 3 mg / L of KH2PO4, 5 to 25 mg / L of NAA and 50 mg / L of sugar alcohol calcium. According to the invention, fluid boron, KH2PO4, NAA and sugar alcohol calcium are selected and compounded, and under natural production and cultivation conditions and dark conditions of simulated storage and transportation, comprehensive and efficient regulation and control schemes for regulating and controlling the period of the bougainvillea speetabilis and inhibiting shedding are respectively screened out; the regulator is applied through exogenous spraying, flowering of the bougainvillea speetabilis can be well promoted or bud falling is inhibited, the ornamental value and storage and transportation resistance of the bougainvillea speetabilis are improved, and a basis is provided for production, cultivation, management and transportation in practical application of the bougainvillea speetabilis.
Owner:CHINA AGRI UNIV SANYA RES INST

A kind of regulator for promoting the flowering of bougainvillea or inhibiting the abscission of flower bud

ActiveCN121369418BBiocideFlowers cultivationPlant regulatorsAnthesis
The present application provides a kind of for promoting bougainvillea spectabilis flowering or inhibiting bud drop regulator, it is related to plant regulator technical field.The regulator of the present application is selected from one or more of 0.1-0.3mg / L fluid boron, 1-3mg / L KH2PO4, 5-25mg / L NAA, 50mg / L calcium sugar alcohol.The present application is selected by fluid boron, KH2PO4, NAA, sugar alcohol calcium, compounded, under the natural production cultivation condition and the dark condition of simulated storage and transportation, respectively, screen out the comprehensive efficient regulation bougainvillea spectabilis flowering, inhibit the regulation scheme of drop;By exogenous spraying to apply regulator, can well promote bougainvillea spectabilis flowering or inhibit bud drop, improve the ornamental and storage and transportation of bougainvillea spectabilis, provide basis for the production cultivation management and transportation in the practical application of bougainvillea spectabilis.
Owner:CHINA AGRI UNIV SANYA RES INST

A method for cultivating Paphiopedilum floridulus

ActiveCN115777480BFlowers cultivationFertilising methodsPaphiopedilum callosumBud
The present invention discloses a method for cultivating a florid Paphiopedilum callosum. The method comprises the following steps: selecting plants with 5-6 leaves, strong growth, and a well-developed root system for root irrigation with a 6-BA solution at a concentration of 200 to 800 mg / L, treating them once every two weeks for a total of 5-6 times, and performing unique fertilizer and water management during the subsequent cultivation process. The present invention utilizes a 6-BA solution to root-irrigate seedlings transplanted for one year to significantly increase the number of tillering buds, and performs appropriate fertilizer and water management during the subsequent cultivation process, which can cause most of the tillering lateral buds and main buds to bloom together, significantly increasing the number of flowers. The method can promote a single Paphiopedilum callosum plant to bloom multiple branches, improve its ornamental value, fill domestic and international gaps, and have a positive driving effect on promoting the development of my country's Paphiopedilum industry.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI +1

Hardwood cutting breeding method of actinidia kolomikta and zizyphus jujuba

ActiveCN119183810BVegetative propogationActinidia polygamaAsexual reproduction
The application provides a hard branch cutting breeding method of Actinidia kolomikta and Zizyphus jujuba, relates to the technical field of asexual reproduction and seedling raising, and comprises the following steps: (1) collecting 1-2 year old branches and pruning branch tips; (2) cutting into 10cm cuttings with more than two bud points, with the upper end of the cutting being inclined and the lower end being horizontal, and soaking the cutting in carbendazim for sterilization treatment; (3) selecting grass charcoal mixed with perlite as a cutting substrate, disinfecting the substrate after being loaded into a plug tray; (4) preparing a rooting agent, treating the cutting of step (2), inserting the cutting into the substrate of the plug tray, fixing the cutting, pouring carbendazim through the substrate, and covering the cutting with a transparent cover; (5) placing the cutting under the condition of 70% shading, increasing 20% illumination after new leaves are unfolded, controlling the temperature to be 10-30 DEG C, timely watering the cutting after cutting, combining with carbendazim sterilization, gradually opening the transparent cover after 40d of cutting, watering according to the situation, and spraying foliar fertilizer every 10d; and (6) transplanting and planting the cutting after 60d of cutting. The application has the beneficial effects of simplifying the hard branch cutting steps of Actinidia kolomikta and Zizyphus jujuba and shortening the cutting breeding period.
Owner:SHANDONG FOREST & GRASS GERMPLASM RESOURCE CENT (SHANDONG YAOXIANG FOREST FARM)

Application of ELF3 gene in regulating growth of poplar terminal buds

The application belongs to the field of genetic engineering, and particularly relates to application of an ELF3 gene in regulating growth of a poplar top bud. Through screening and testing, it is found that knocking out the ELF3 gene can achieve the technical effect of delaying growth stop and dormancy time of a tree top bud under short-day conditions and promoting tree growth.
Owner:HUAZHONG AGRI UNIV

Method for extracting white birch overwintering bud cell nucleus

The invention discloses a method for extracting cell nucleuses of white birch overwintering buds, and belongs to the technical field of extraction of cell nucleuses of white birch overwintering buds. The invention aims to solve the problems of cell nucleus extraction and polysaccharide and polyphenol removal. The invention provides a method for extracting white birch overwintering bud cell nucleuses, which comprises the following steps: grinding white birch overwintering buds with liquid nitrogen, then carrying out solution and resuspension for several times, then carrying out density gradient centrifugation, and then filtering with Miracloth filter cloth to finally obtain the white birch overwintering bud cell nucleuses. ChIP-seq or CUTamp which is suitable for the overwintering buds is explored; the Tag system solves the problems of cell nucleus extraction and polysaccharide and polyphenol removal, and the state of the white birch bud chromatin can be analyzed.
Owner:NORTHEAST FORESTRY UNIV

Method for inducing polyploidy of radix peucedani by using fasciculate buds and application thereof

ActiveCN120898725Bprevent oxidationReduce the incidence of browningBiotechnologyColchicine
The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.
Owner:HANJIANG NORMAL UNIV

Thrip resistance identification device and application

PendingCN121667172AAnimal husbandryBiotechnologyWild species
The invention discloses a thrip resistance identification device and application. The device comprises an inoculation barrel, a preservation substrate is laid at the bottom in the inoculation barrel, an isolation structure with one or more through holes covers the preservation substrate, and a sealing structure with one or more air holes covers an opening in the top of the inoculation barrel. When the device is used, the whole plant does not need to be cultivated, in-vitro flowering branches can be picked for identification, rapid screening of large-scale germplasm resources can be achieved, and repeatability and consistency of experimental results are good. Meanwhile, when the device is used, buds and functional leaves of in-vitro flowering branches are reserved, the actual feeding part of the Frankliniella occidentalis is simulated, and compared with in-vitro leaf inoculation, the real resistance level can be better reflected. The device has the advantages of easily available materials, low cost and strong operability, can be used for identifying the resistance of the Frankliniella occidentalis of various materials such as chrysanthemum and related wild species, cultivated species, breeding offspring and the like, provides a high-quality parent material for insect-resistant breeding, can be used for researching an insect-resistant mechanism, and is suitable for popularization and application in basic breeding units.
Owner:NANJING AGRICULTURAL UNIVERSITY

A tissue culture propagation method of eriochloa villosa

ActiveCN118402471BGrowth substratesCulture mediaEriochloa villosaEriochloa
The application discloses a tissue culture propagation method of eriochloa contracta. The creeping stem section of the eriochloa contracta is collected, washed, and then sterilized and dried; the sterilized and treated stem section is inoculated into a bud induction medium of MS+1-1.5 mg / L 6-BA+0.1-0.5 mg / L NAA+30 mg / L sucrose+6.5 mg / L agar to perform bud induction, and after 15-20 days of culture, the adventitious bud seedling is obtained; the adventitious bud of about 1-1.5 cm induced in the primary culture medium is inoculated into a proliferation culture medium to perform subculture, and the next step can be divided into single bud expansion or direct transplanting domestication. The two-step method only needs primary culture-proliferation and rooting to be simultaneously performed, and the transplanting survival rate is high.
Owner:JIANGXI ACAD OF WATER RESOURCES (JIANGXI PROVINCE DAM SAFETY MANAGEMENT CENT JIANGXI PROVINCE WATER RESOURCES MANAGEMENT CENT) +1

Intelligent identification and calculation method for growth phenotypic characteristics of edible fungi in germination period

The invention discloses an intelligent identification and calculation method for growth phenotypic characteristics of edible fungi in a budding period, and relates to the technical field of modern facility agriculture. Comprising the following steps: shooting and obtaining a clear RGB image of field growth in the edible mushroom germination period; performing contrast enhancement processing on the clear RGB image; accurate identification is carried out through an improved instance segmentation model RSL-YOLOv8m-Seg to obtain a bud primordium mask image; traversing and segmenting each bud primordium mask one by one, and extracting the contour of the mask; reconstructing a bud primordium contour by adopting a contour fitting method based on each mask contour; extracting morphological characteristics of the bud primordium; and counting growth phenotypic characteristics in a budding period. According to the method, the growth characteristics of the edible fungi in the germination period can be accurately calculated, and a phenotypic characteristic basis is provided for intelligent and accurate management and control of an edible fungi house.
Owner:SHANGHAI SECOND POLYTECHNIC UNIVERSITY +1

Efficient quality-guaranteeing processing method of lilies

The invention discloses an efficient quality-guaranteeing processing method for lilies. The efficient quality-guaranteeing processing method comprises the following steps of harvesting and sorting, staged drying, gradient drying, homogenizing and moisture regaining, packaging, final sorting after moisture regaining, elimination of individuals which are not completely dried, and then sealed packaging. According to the efficient quality-guaranteeing processing method for the lilies, firstly, through strict harvesting standards and field sorting, the uniformity of the quality of the raw materials is guaranteed from the source, and a good foundation is laid for subsequent processing; and secondly, a strategy of treating flowers and flower buds in stages is adopted, and a gradient drying process with multi-stage precise control is combined, so that efficient and mild dehydration can be realized according to the characteristics of different materials, and the natural color, form and effective components of the lilies are reserved to the greatest extent. And finally, through a unique homogenizing and moisture regaining process, the internal moisture of the finished flowers is effectively balanced, the problem of local mildew or fragility is avoided, and the stability, the storage safety and the commodity appearance of the product are remarkably improved.
Owner:LINTAO COUNTY FENJIWAN LILY FARMERS PROFESSIONAL COOP

A rapid induction medium and method from kiwifruit leaf pieces to whole plants

ActiveCN118696824BSucroseBud
This invention relates to a rapid induction culture medium and method for inducing bud formation from kiwifruit leaves to complete plants, belonging to the field of biotechnology. The callus induction culture medium for rapidly inducing bud formation from kiwifruit leaves includes MS medium, sucrose, 6-benzylaminopurine, naphthaleneacetic acid, thidiazuron, and agar, with the following weight ratios: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.000001%-0.000003%, naphthaleneacetic acid 0.0000002%-0.0000008%, thidiazuron 0.000001%-0.000003%, agar 0.006%-0.01%, and the balance being water. The formula for the callus induction bud culture medium is as follows: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.0000005%-0.000003%, naphthaleneacetic acid 0.00000005%-0.0000002%, agar 0.006%-0.01%, with the balance being water. This invention provides a rapid induction culture medium and method for kiwifruit leaves to complete plants, effectively inhibiting problems such as callus waterlogging, browning, and vitrification. It not only increases the number of buds but also improves the quality of buds, which is beneficial to the growth and differentiation of callus tissue, making it easier for it to differentiate into buds, thus laying a solid foundation for subsequent genetic transformation experiments.
Owner:ZHEJIANG FORESTRY UNIVERSITY

A method for high-frequency regeneration of rhododendron anthopogon based on cotyledon node indirect organogenesis

The application discloses a method for high-frequency regeneration of Rhododendron chrysanthum based on cotyledon node indirect organogenesis, and belongs to the field of plant biotechnology. The method takes the cotyledon node of Rhododendron chrysanthum sterile seedling as an explant, and obtains a complete regenerated plant through the induction of callus, differentiation of adventitious buds, elongation of adventitious buds, strong seedling and rooting culture in sequence. The application realizes the super-high-frequency differentiation of adventitious buds by combining the cotyledon node explant with a specific hormone combination for the first time, the differentiation coefficient is as high as 96, and the rooting rate is 92.7%, thereby effectively solving the technical bottleneck of low propagation efficiency of Rhododendron chrysanthum, and providing reliable technical support for the conservation and industrialization development of Rhododendron chrysanthum germplasm resources.
Owner:BEIJING FORESTRY UNIVERSITY

Method for rapidly restoring Phragmites australis population in degraded land through stolon domestication

A method for rapidly restoring Phragmites australis population in degraded land through stolon domestication is provided, including the following steps: at the beginning of the Phragmites australis growing season from May to June every year, the Phragmites australis is made to crawl by draining water and artificially crushing the aboveground stems, and then the sprouting of stolon internode buds or root buds is promoted by continuously controlling water; in mid-July, suitable stolons containing internode buds or root buds are selected, and the root segments are dug from the base, and the stolons are wound in a circle in the extension direction and fixed, and sprayed with water to keep moisture; the fixed stolons are unfolded and transplanted into degraded land for daily water and fertilizer management.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Hydrangea plant named ‘Naga 4’

A new cultivar of Hydrangea serrata plant named ‘Naga 4’ that is characterized by its unique foliage colors; young foliage in summer is tri-color green with white margins and flushed with bright pink and mature foliage that is green with white margins, its blooms present from mid-June to August in The Netherlands, its pink flower buds that open into pink flowers with a light pink and white centers, and its suitability as a container plant.
Owner:OSCO GARDEN

A method for obtaining high frequency embryogenic material of plants

The present application belongs to the field of plant tissue culture and genetic transformation technology, and particularly relates to a method for obtaining high-frequency embryogenic material of plants. The method comprises the following steps: inoculating plant explants on a callus induction medium for culture; continuously subculturing the callus for 4 to 6 times; transferring the embryogenic callus grown after subculture to a sprouting medium for culture, and inducing the embryogenic callus to form adventitious buds on the sprouting medium; transferring the adventitious buds to a rooting medium for culture until roots are formed; transplanting the plants with roots, and culturing until maturity and fruiting to obtain the high-frequency embryogenic material. The method provided by the present application can inoculate mature embryos of low-frequency embryogenic material on an induction medium to induce the formation of embryogenic callus, and then regenerate and induce the embryogenic callus to form seedlings, and through one or more repeated screening, the high-frequency embryogenic material under the genotype background can be obtained.
Owner:SHANXI AGRI UNIV

Corydalis pygmaea terminal bud organogenesis and in-vitro rapid propagation method

PendingCN121549272APlant tissue cultureHorticulture methodsOrganogenesisBud
The invention provides a corydalis pygmaea terminal bud organogenesis and in-vitro rapid propagation method, and belongs to the technical field of plant tissue culture. Comprising the following steps: by taking a terminal bud of a corydalis pygmaea aseptic seedling as an explant, sequentially performing adventitious bud differentiation culture, cluster bud multiplication culture and rooting induction to obtain a corydalis pygmaea tissue culture seedling, pretreating a root system of the corydalis pygmaea tissue culture seedling with nano-zinc oxide, and transplanting the corydalis pygmaea tissue culture seedling. According to the method, the terminal bud is used as the explant, the organ occurrence rate and the propagation coefficient are remarkably increased by optimizing the culture medium formula and the culture conditions, the induction rate reaches 85% or above, the browning rate is controlled to be 10% or below, the proliferation multiple per four weeks can reach 11-14 times, the rooting rate exceeds 90%, rapid and efficient propagation of corydalis pygmaea is achieved, reliable technical support is provided for large-scale production, and the method has a wide application prospect. The problem of raw material supply can be effectively solved, and a foundation is laid for industrial production of active ingredients.
Owner:INSTITUTE OF CHINESE MATERIA MEDICA CHINA ACADEMY OF CHINESE MEDICAL SCIENCES +1

Method for inducing orchid gene silencing by virus

The invention discloses a method for inducing orchid gene silencing through viruses. According to the method provided by the invention, orchid buds are used as acceptor materials; the construction method comprises the following steps: constructing a silent vector containing 200-300bp target genes by taking Super-MV-pro60 as a basic vector, and introducing the silent vector into agrobacterium to obtain an engineering bacterium; and injecting the engineering bacterium liquid into the buds, and culturing. According to the method, efficient silencing of orchid genes can be achieved, the silencing success rate is 75% or above, the silencing efficiency is 60%-95%, and the method is good in stability and has a good silencing effect under different environmental conditions. The method provided by the invention provides a new technical means for orchid gene function identification, realizes efficient silencing of orchid genes, can be used for rapid verification of orchid flower color, flower fragrance, flower type and other gene functions, and has important significance for orchid function gene identification and cultivation of new orchid varieties through a molecular breeding technology.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Method for improving transplanting survival rate of taiwania flousiana container seedlings

The invention discloses a method for improving the transplanting survival rate of taiwania flousiana container seedlings, and relates to the technical field of seedling cultivation, and the method comprises the following steps: selecting a plot with smooth gradient and good ventilation and illumination, and preparing sterile deep nutrient soil with proper sand grain content; selecting taiwania flousiana seedlings with plump terminal buds and healthy root systems, and performing sterilization and insect killing treatment on the land parcels after ladder opening and soil preparation; nutrient soil is bagged and placed normatively, a shed frame space is reserved, and an elevated arched shed is built and covered with a shed film and a high-sunshade-degree sunshade net; the nutrition bags are watered for the first time until the humidity reaches the standard, nursery stocks are planted after being watered before being lifted, moisturized and transported and subjected to field root pruning, root fixing water is watered at nightfall, and then a greenhouse opening is closed; the method solves the problems of easy damage of root systems, lack of environmental regulation and the like in traditional transplantation, reduces the activity loss of nursery stocks and the risk of plant diseases and insect pests, and significantly improves the transplantation survival rate.
Owner:赵军

Lantana plant named ‘KLELA24691’

ActiveUSPP37644P2BudLantana
A new Lantana plant particularly distinguished by having an excellent branching and mounding habit, a flower color that changes during flower development from orange buds to yellow-orange when the flower opens, and to bright magenta as the final color, and large flowers with continuous flowering from April through November, is disclosed.
Owner:KLEMM & SOHN GMBH & CO KG

A method for semi-wild three-dimensional cultivation of ginger and Ganoderma lucidum in newly afforested areas in the Lingnan region

The application discloses a kind of new afforestation land of Lingnan region rhizoma zingiberis and ganoderma lucidum imitated wild three-dimensional planting method, which comprises the following steps: 1) selecting the new afforestation land of Lingnan region planted with trees within 2 years;2) ridging between the tree rows of new afforestation land, digging ganoderma lucidum planting ditch on the ridge, placing ganoderma lucidum stick horizontally in the ganoderma lucidum planting ditch, connecting head to tail between sticks, covering with broken soil, covering soil layer on the stick, covering dry grass on the soil layer, and carrying out ganoderma lucidum imitated wild planting;3) in April-May, digging hole between ganoderma lucidum sticks, putting one section of rhizome of zingiber officinale rose into each hole after mixing with organic fertilizer and soil, putting bud head upward, filling fine topsoil while putting, and carrying out zingiber officinale imitated wild planting;4) regional weeding and zingiber officinale fertilization;5) ganoderma lucidum and zingiber officinale harvesting.The method can not only realize ganoderma lucidum and zingiber officinale imitated wild planting in new afforestation land, but also improve the utilization rate of new afforestation land, achieve the purpose of cultivation instead of rearing and short-term cultivation instead of long-term cultivation, realize three-dimensional planting of new afforestation land, and improve economic benefit.
Owner:GUANGXI FORESTRY RES INST

Flower forcing method for erythrina variegata in park green land

PendingCN120304232AFertilising methodsCultivating equipmentsIndian coral treeBud
The invention discloses a flower forcing method for erythrina variegata in a park green land. The method comprises the steps of branch pruning, soil fertilization and water management. The flowering induction of the Erythrina variegata is realized through the measures of pruning a large amount of the Erythrina variegata, fertilizing soil, reducing watering times and watering quantity and the like. According to the flower forcing method, the illumination intensity and area of leaves in the crown of the erythrina coralis tree can be improved, the content of nutrient substances in the erythrina coralis tree body is increased, flower bud differentiation is promoted, and therefore the ornamental value of the erythrina coralis is improved.
Owner:HUAQIAO UNIVERSITY