The invention relates to a cultivation substrate for Camellia oleifera seedlings. The cultivation substrate is composed of the following raw materials in parts by weight: 20 to 45 parts of yellow heart soil, 15 to 24 parts of sugarcane chips, 8 to 15 parts of bean cake, 10 to 20 parts of mushroom dregs, 5-12 parts of manure, 3-10 parts of walnut cattail shell, 2-10 parts of silkworm excrement, 1-4 parts of fulvic acid, 5-10 parts of biogas slurry, 0.5-2 parts of borax, 3-15 parts of silicon fertilizer, phosphoric acid Potassium dihydrogen 1-10 parts, appropriate amount of water. The camellia oleifera seedling cultivation substrate of the present invention has a wide range of sources, fully utilizes waste resources such as sugarcane scraps, bean cakes, mushroom dregs, and cow dung, has low cost, and broadens the way of waste utilization; the raw materials are reasonably matched and comprehensive in nutrition, and not only contain nitrogen, phosphorus, potassium, etc. Quick-acting nutrient, containing silicon, calcium, magnesium and other medium element fertilizers as well as organic matter and fulvic acid, is a non-toxic fertilizer with both quick-acting and slow-release properties, which can promote the growth of Camellia oleifera seedlings and increase the yield of Camellia oleifera; preparation The method is simple and easy to operate.