Process condition for extracting proteins of Northeast China carya cathayensis mandshurica seeds

A process condition and technology of walnut catalpa, which is applied in the field of extracting protein from Northeast hickory catalpa seed kernels, can solve the problems of rare research on protein extraction of Northeast hickory catalpa, and achieve the effects of cost saving, good resolubility, and resource saving

Inactive Publication Date: 2013-07-03
NORTHEAST FORESTRY UNIVERSITY +3
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there are few studies on t...

Method used

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  • Process condition for extracting proteins of Northeast China carya cathayensis mandshurica seeds

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Embodiment 1

[0029] After crushing 1Kg of dried seed kernels through a 40-mesh sieve, pass through supercritical CO 2 Degreasing, the operating conditions are pressure 35MPa, temperature 40°C, time 2.5 hours, a total of 3 extractions to fully remove grease. Add 0.01mol / L NaOH extract (solid-liquid ratio 1 / 30) to the degreased Hickory catalpa powder, stir and extract in a 45°C water bath for 1.5h, centrifuge and continue to extract 1.5h with the extract under the same conditions for precipitation. Centrifuge and combine the two extraction supernatants. It was determined that the protein extraction rate reached 84.36%. Use 0.1mol / L HCl and NaOH solution to adjust the pH of the extracted protein solution to the isoelectric point of 4.5, stir evenly to fully precipitate the protein, and centrifuge again. Take the precipitate, adjust the pH of the precipitate to neutral, and then use dialysis to remove the salt ions in the protein solution. The protein after dialysis and desalination is vacu...

Embodiment 2

[0033] After crushing 1Kg of dried seed kernels through a 40-mesh sieve, pass through supercritical CO 2 Degreasing, the operating conditions are pressure 35MPa, temperature 40°C, time 2.5 hours, a total of 3 extractions to fully remove grease. Add 0.01mol / L NaOH extract (solid-liquid ratio 1 / 30) to the degreased Hickory catalpa powder, stir and extract in a 45°C water bath for 2 hours, centrifuge and continue to extract the precipitate for 2 hours under the same conditions with the extract, centrifuge, Combine the two extraction supernatants. It was determined that the protein extraction rate reached 83.17%. Use 0.1mol / L HCl and NaOH solution to adjust the pH of the extracted protein solution to the isoelectric point of 4.5, stir evenly to fully precipitate the protein, and centrifuge again. Take the precipitate, adjust the pH of the precipitate to neutral, and then use dialysis to remove the salt ions in the protein solution. The protein after dialysis and desalination is...

Embodiment 3

[0035] After crushing 1Kg of dried seed kernels through a 40-mesh sieve, pass through supercritical CO 2 Degreasing, the operating conditions are pressure 35MPa, temperature 40°C, time 2.5 hours, a total of 3 extractions to fully remove grease. Add 0.02mol / L NaOH extract (solid-liquid ratio 1 / 30) to the degreased Hickory catalpa powder, stir and extract in a 50°C water bath for 1.5h, centrifuge and continue to extract 1.5h with the extract under the same conditions for precipitation. Centrifuge and combine the two extraction supernatants. It was determined that the protein extraction rate reached 88.98%. Use 0.1mol / L HCl and NaOH solution to adjust the pH of the extracted protein solution to the isoelectric point of 4.5, stir evenly to fully precipitate the protein, and centrifuge again. Take the precipitate, adjust the pH of the precipitate to neutral, and then use dialysis to remove the salt ions in the protein solution. The protein after dialysis and desalination is vacu...

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Abstract

The invention relates to a process condition for extracting the proteins of Northeast China carya cathayensis mandshurica seeds. The process comprises the following steps of: taking the Northeast China carya cathayensis mandshurica seeds as raw materials, and obtaining the defatted carya cathayensis mandshurica seed residues after removing seed skins, crushing, and repeatedly defatting for three times by supercritical CO2; performing protein extraction on the defatted carya cathayensis mandshurica seed residues by NaOH solution with a certain concentration, and centrifuging the extracting solution and taking the supernatant, and then obtaining the carya cathayensis mandshurica protein solution; and adjusting the pH value of the supernatant to be 4.5, and adequately precipitating proteins, centrifuging, reserving the precipitate, and adjusting the pH value of the precipitate to be 7, desalting for 36 hours by dialyzing, freeze-drying the dialyzed product, and then obtaining the proteins of the Northeast China carya cathayensis mandshurica seeds. According to the process disclosed by the invention, the defatted Northeast China carya cathayensis mandshurica seed residues in the industry can be used as raw materials, thus creating a new value while saving cost; and moreover, the process disclosed by the invention is simple, high in the yield of the carya cathayensis mandshurica proteins and strong in operability, is the premise of further development and utilization for the carya cathayensis mandshurica seed proteins, and is suitable for industrialized production.

Description

technical field [0001] The invention relates to a process condition for extracting protein from pecan catalpa seed kernels. Background technique [0002] Hickory catalpa (Juglans Mandshurica Maxim) is produced in the Heilongjiang Xiaoxing'an Mountains below an altitude of 500-800m, and the eastern mountainous areas, Jilin, Liaoning, eastern Inner Mongolia, Hebei, Shanxi and other places. Hickory nuts have great use value in wood, food and medicine. As a nutritious nut, hickory catalpa not only has delicious taste and unique flavor, but also has unique nutritional components and functional effects. Seed kernels are rich in protein, a variety of essential fatty acids and 22 mineral elements such as calcium, magnesium, phosphorus, zinc and iron that are necessary for the human body. Its protein digestibility reaches 87.2%, 18 kinds of amino acids are complete, and the proportion of 8 kinds of essential amino acids required by the human body is suitable, and the contents of gl...

Claims

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Application Information

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IPC IPC(8): C07K1/30C07K1/14
CPCY02P20/54
Inventor 包怡红于阳阳刘爽
Owner NORTHEAST FORESTRY UNIVERSITY
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