120 results about "Seed protein" patented technology

The invention discloses an application of a soybean ZF-HD protein coding gene GmZFHD11 in the aspect of influencing the quality of soybean seeds. The nucleotide sequence of the soybean ZF-HD protein coding gene GmZFHD11 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the soybean ZF-HD protein coding The constructed plant overexpression vector pBA002-GmZFHD11 is subjected to agrobacterium tumefaciens-mediated soybean genetic transformation to obtain a soybean receptor variety Jack, and it is found that compared with a receptor control group, the content of sulfur-containing amino acid in seeds is increased, the content of protein is increased, the content of beta-conglycinin (7S) is reduced, the content of glycinin (11S) is increased, and the 11S / 7S ratio is increased; and the quality of the overexpressed transgenic soybean strain seed protein is improved. Therefore, the gene can be introduced into plants as a target gene to improve the quality of soybean grain protein.

The invention discloses a cross-platform soybean seed protein content estimation method based on a spectral time sequence. The method comprises the following steps: step 1, acquiring high spectral data of a soybean canopy; 2, collecting a soybean multispectral image; step 3, acquiring soybean physiological parameters and grain protein content data; step 4, obtaining soybean photosynthetic parameter data; step 5, calculating a vegetation index for estimation; step 6, constructing a vegetation index time sequence; 7, screening an optimal time window; 8, extracting dynamic characteristics; step 9, establishing a model, estimating the soybean protein content, and performing precision verification; and step 10, carrying out mobility analysis on the model from a near-ground canopy to a satellite platform. According to the method, a theoretical basis and a practical path are provided for realizing remote sensing-based soybean GPC accurate monitoring, wide application of the method on a satellite remote sensing platform can be promoted in the future, and high-precision, high-time-efficiency and large-scale soybean GPC estimation is realized.

The invention provides a weight-losing formula containing medicinal and edible components, and belongs to the technical field of medicines. The weight-losing formula comprises the following components in parts by weight: 35-50 parts of oat kernels, 10-12 parts of poria cocos, 20-30 parts of coix seeds, 5-8 parts of radix ophiopogonis, 10-15 parts of codonopsis pilosula, 5-8 parts of Chinese wolfberry fruits, 1-2 parts of dried orange peel, 10-15 parts of Chinese yams, 8-12 parts of haws, 10-16 parts of astragalus membranaceus, 3-5 parts of purple perilla, 15-20 parts of soybean protein isolate, 10-15 parts of red beans and 10-15 parts of fructus cannabis protein peptides. According to the weight-losing formula disclosed by the invention, natural medicinal material synergy is taken as a core, 14 components are compounded according to a specific ratio, a synergistic and complementary effect can be realized, and a large number of experiments prove that the weight-losing formula provided by the invention has the effects of comprehensively improving and controlling the weight, reducing the fat coefficient, improving lipid metabolism and preventing weight rebound. Moreover, the food is better in eating experience and safety, and suitable for long-term eating.

The invention discloses a composition for regulating body weight through satiety-sugar control double channels. The composition is prepared from the following components in parts by weight: 5-25 parts of cinnamon extract, 1-20 parts of ganoderma lucidum EGCG composite powder, 10-40 parts of L-arabinose, 5-40 parts of composite fruit and vegetable fiber powder, 5-25 parts of fructus cannabis protein peptide, 1-20 parts of apple polyphenol, 1-20 parts of white kidney bean dietary fiber powder and 1-20 parts of agaricus bisporus powder. The composition is characterized in that digestion and absorption of carbohydrate are inhibited, the swelling ratio larger than or equal to 35 times is achieved through a composite fiber system, gastric emptying is remarkably prolonged, satiety hormones such as GLP-1 are adjusted, and the composition can be used as a non-alternative weight management product and is good in safety.

The invention is suitable for the technical field of health care, and provides a health-care food formula for improving sleep. Comprising the following raw materials in parts by weight: 150-250 parts of whole-grain oatmeal, 100-200 parts of germinated brown rice powder, 60-80 parts of pumpkin seeds, 50-60 parts of black sesame, 40-50 parts of flaxseeds, 62-70 parts of dried sour cherries, 55-60 parts of dried figs, 45-52 parts of dried bananas, 20-25 parts of chamomile flower powder, 18-20 parts of ground cinnamon, 50-60 parts of fructus cannabis protein powder, 70-80 parts of millet powder, 35-40 parts of hericium erinaceus powder and 18-20 parts of ganoderma lucidum spore powder. The beverage is prepared from 48-50 parts of fructo-oligosaccharide, 35-40 parts of date palm paste, 25-30 parts of beer yeast powder, 28-30 parts of walnut oil, 15-20 parts of semen ziziphi spinosae and 10-15 parts of semen boitae. Various nutritional foods are adopted, so that the problem of difficulty in falling asleep caused by insufficient secretion of melatonin can be solved, and anxiety, light sleep caused by excessive activity of brain waves and the like can be relieved.

The invention discloses an application of a GmVAP27-3 gene in regulating and controlling the protein content of soybean seeds or regulating and controlling the oil content of the soybean seeds, and belongs to the technical field of soybean quality. The invention aims to improve the quality of soybeans and increase the protein content and the grease content of soybean seeds. The invention provides application of GmVAP27-3A protein or GmVAP27-3B protein in regulation and control of the protein content of soybean seeds, regulation and control of the oil content of the soybean seeds, regulation and control of the plant height of the soybean or regulation and control of the size of the soybean seeds. The soybean quality can be improved, and a scientific strategy and basis are provided for genetic improvement of the soybean.

The invention discloses a GmGID1-2 gene molecular marker obviously associated with soybean plant height, yield, grease content and protein content and application of the GmGID1-2 gene molecular marker. The molecular marker contains an SNP (Single Nucleotide Polymorphism) site obviously associated with soybean plant height, yield, grease content and protein content, the site is located in a GmGID1-2 gene promoter region, Wm82. A2. V1 is taken as a reference genome, the site is located at 36395036bp of a soybean No.3 chromosome, allelic variation is carried out to obtain a basic group C or T, and the nucleotide sequence is shown in SEQ ID NO: 1. Compared with the soybean with the genotype T, the soybean with the genotype C has the advantages of lower plant height, lower seed protein content, higher yield and higher seed oil content. The dCAPS molecular marker developed according to the SNP can be used for molecular marker-assisted selection, the soybean breeding efficiency is improved, and the soybean breeding process is accelerated.

The invention provides a preparation method and application of fructus cannabis protein peptide with a sugar absorption blocking effect, and belongs to the technical field of functional active peptides, and the preparation method comprises the following steps: crushing fructus cannabis, and degreasing through low-temperature continuous phase change to obtain degreased fructus cannabis meal after degreasing; the degreased hemp seed meal is hydrolyzed through polysaccharase and protease in sequence, low-temperature salting-out is carried out after hydrolysis is finished, drying and sterilization are carried out, and the hemp seed protein peptide is obtained. The fructus cannabis protein peptide obtained by the technical scheme and the preparation method disclosed by the invention has the following effects: 1, the fructus cannabis protein peptide prepared by the preparation method disclosed by the invention can be used for remarkably inhibiting the activity of alpha-amylase and alpha-glycosidase (the maximum inhibition rates can respectively reach 84.2% and 75.1%), so that digestion and absorption of a human body on carbohydrates such as starch, dextrin and disaccharide are blocked; and 2, double-blind clinical test results show that the fructus cannabis protein peptide prepared by the invention can effectively reduce the postprandial blood sugar peak value and peak area, and the effective sugar breaking rate can reach 30-60%.

The invention discloses trichosanthes kirilowii maxim seed polypeptide with alpha-glucosidase inhibitory activity and a preparation method thereof. Four Trichosanthes kirilowii Maxim seed polypeptides with alpha-glucosidase inhibitory activity are obtained through Trichosanthes kirilowii Maxim seed protein preparation, enzymolysis, separation and purification and identification, and the sequences of the four Trichosanthes kirilowii Maxim seed polypeptides are respectively HRQTWD, HANRLP, FGGVGER and SYAPEDR. Molecular docking analysis results show that interaction between the trichosanthes kirilowii maxim seed hydrolytic peptide and amino acid residues of an alpha-glucosidase active pocket is mainly based on hydrogen bonds, electrostatic interaction and hydrophobic interaction, and residues D203, D327, D443, F450, D542 and F575 are key binding sites interacting with the four polypeptides. The trichosanthes kirilowii maxim seed polypeptide with alpha-glucosidase inhibitory activity prepared by the invention can be used as a food-borne hypoglycemic component to be used for developing health-care foods and medicines for reducing blood sugar.

The invention discloses a molecular marker for identifying the protein content of soybean seeds and application of the molecular marker. The invention further discloses an SNP site related to the soybean seed protein content, the SNP site is located at the 16083582nd site of the No.4 chromosome of a soybean genome, and the polymorphism is G or A. The invention further develops a molecular marker for identifying the soybean seed protein content on the basis of the SNP site and verifies the molecular marker in a four-way recombinant inbred line material. A verification result shows that the found SNP site is remarkably related to the soybean seed protein content character, and the seed protein content of a Hap1 genotype soybean material with the SNP site being G is remarkably higher than that of a Hap2 genotype soybean material with the SNP site being A. The invention provides a theoretical basis for soybean molecule-assisted breeding, and has important significance for cultivating high-quality new varieties.

The invention belongs to the technical field of biology, and relates to a method for preparing peony seed protein from an oil peony byproduct. The peony seed protein is obtained by taking oil peony seed meal as a raw material and adopting a separation and purification method of protease enzymolysis, saccharifying enzyme desugaring, centrifugation, microfiltration and ultrafiltration. The peony seed protein disclosed by the invention is low in alkali consumption and dosage, simple to operate and high in solubility.

Genetically modified plants having improved agronomic traits are disclosed. The plants comprise nucleic acid modifications that modify CCT proteins thereby improving the agronomic trait of the plants, including seed quality, seed oil content and seed protein content. Nucleic acid modification systems and nucleic acid constructs encoding the engineered nucleic acid modification system are also disclosed. Further, methods of improving agronomic traits of plants using the nucleic acid modification systems and nucleic acid constructs.

The application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a molecular marker related to soybean seed protein content and application thereof, a nucleotide sequence of the molecular marker is shown as SEQ ID NO. 1, and is located in the interval of 32403844 bp-32403863 bp of the 16th chromosome of soybean. The QTL interval related to the soybean seed protein content identified by the application has a size of 0.076 Mb, the number of genes in the interval is small, and important foundation is provided for related gene mining. On the basis, one molecular marker related to the soybean seed protein content, namely SSR-16-1082, is developed in the interval, the molecular marker has high correlation degree with the soybean seed protein content, and important marker information is provided for molecular marker assisted selection breeding of the soybean seed protein content.

The application belongs to the technical field of protein extraction, and provides a method for improving water holding property and oil holding property of peony seed protein, the core of the method is that before the extraction of the peony seed protein, the peony seed is pretreated by the following method: the peony seed meal is subjected to steam explosion treatment under the condition of 0.4-1.2 Mpa and 60-150 s, preferably 0.8 Mpa and 100 s, to obtain the steam exploded peony seed, and the sample after the steam explosion is subjected to drying treatment; preferably heat pump drying. The peony seed protein prepared by the method has obviously improved water holding property, oil holding property, foaming property, foam stability, emulsifying property and emulsion stability; the valine is increased by 8.58%, the leucine is increased by 6.63%, and the tyrosine is increased by 10.65%; the extraction rate of the seed protein is 76.82%, and the extraction effect is excellent.

This invention discloses a hair repair composition for permed hair, comprising, by weight: 0.02-1.0 parts hydrolyzed plant protein, 0.1-2.0 parts biomimetic amino acid complex, 50-95 parts carrier, and 0.5-15 parts additives. The hydrolyzed plant protein is obtained from Moringa seed protein through multi-enzyme synergistic hydrolysis, with a molecular weight less than 3500 Daltons. The biomimetic amino acid complex includes at least wheat amino acids, soybean amino acids, arginine hydrochloride, serine, and threonine. Under weakly acidic conditions, this composition can simultaneously repair broken disulfide bonds, hydrogen bonds, and ionic bonds in hair. The hydrolyzed plant protein provides disulfide bond reconnection sites, and the biomimetic amino acid complex constructs a multi-point weak bond network through hydroxyl and ionic groups, achieving "triple bond repair" synergistically. This invention can significantly improve the mechanical strength, elasticity, and breakage resistance of damaged hair, with long-lasting, gentle, and safe repair effects, suitable for daily care of damaged hair after perming and dyeing.

The invention discloses China-hemp peptide multi-nutrient protein powder with a metabolism improving function, which comprises China-hemp seeds, soybean protein isolate, China-hemp seed protein, chickpea protein, pea protein, China-hemp peptide, rice protein, broad bean protein, mung bean protein and wheat hydrolyzed protein. Wherein the Chinese hemp seeds are 15-25 parts, the soybean protein isolate is 10-20 parts, the Chinese hemp seed protein is 5-15 parts, the chickpea protein is 8-12 parts, the pea protein is 8-12 parts, the Chinese hemp peptide is 8-15 parts, the rice protein is 5-10 parts, the broad bean protein is 5-10 parts, the mung bean protein is 5-10 parts, and the like. According to the present invention, besides the protein nutrition, the small molecule peptide and 10 other nutrients such as Omega3, gamma-linolenic acid, vitamin E and a variety of minerals are contained, such that the complete protein nutrition is provided, and the health-care food is rich in Omega-3 fatty acid, gamma-linolenic acid, vitamin E, potassium, magnesium, zinc and other minerals with different molecular magnitudes extracted from China-hemp seeds, the ten nutrients, a plurality of plant proteins and small molecule peptides have a synergistic effect to jointly form a basis for improving the metabolic function.

This invention relates to the soybean GmFAH gene and its application in improving seed protein content and branching number, belonging to the field of plant genetic engineering technology. The protein encoded by the soybean GmFAH gene is shown in SEQ ID NO.1. The encoding DNA sequence is shown in SEQ ID NO.2. The application of the soybean GmFAH gene in improving seed protein content and branching number is as follows: The method of this invention involves introducing the GmFAH gene DNA molecule into recipient soybeans to obtain transgenic soybeans. The protein content of the transgenic soybean seeds is significantly higher than that of the recipient soybeans, and the number of branches is significantly greater, while the grain weight and yield are not significantly different from those of the recipient soybeans. Using the method of this invention, the protein quality and plant type traits of soybean seeds can be improved, accelerating the efficiency of molecular breeding for superior soybean quality and ideal plant type.

The present application belongs to the technical field of food processing, and relates to a method for removing earthy smell of tilapia fillets. The method comprises the following steps: covering the surface of the tilapia fillets with pleurotus eryngii powder, and placing the tilapia fillets in a deodorizing and fresh-keeping liquid for 30-60 min; the deodorizing and fresh-keeping liquid is composed of ginger powder 15-20 parts, cannabis seed protein 5-10 parts, lysozyme 0.5-1 part, tea polyphenol 4-8 parts and water 60-100 parts in terms of mass fraction. The present application solves the technical problem of long-term maintenance of fresh fish flavor when removing the earthy smell of tilapia fillets.

The application provides a composite biological flocculant and a preparation method and application thereof, the flocculant is composed of 20-40 parts of flocculation bacteria fermentation extract, 5-10 parts of modified plant-based flocculation component, 8-18 parts of cationized nanocellulose, 5-15 parts of porous mineral powder and 2-6 parts of biological surfactant. The application adopts bacillus licheniformis and candida utilis double-strain synergistic fermentation to produce flocculation, and respectively modifies the jatropha seed protein and nanocellulose, and through gradient compounding process, the biological components are uniformly loaded on the surface of inorganic carrier to form a composite system. The application solves the problems of low activity, slow settling and single function of the existing biological flocculant, the removal rates of turbidity, COD, total phosphorus and heavy metal are respectively ≥96%, 85%, 92% and 97%, the settling speed is increased by 2-3 times, there is no secondary pollution, and the application is suitable for purification treatment of municipal sewage, industrial wastewater and various water bodies.

The application discloses a SNP site related to soybean protein content and application thereof. The SNP site related to soybean protein content disclosed by the application is located at 35708082 of chromosome 19 of a soybean genome, and the polymorphism is C or A. The application also discloses a HRM melting curve method for identifying soybean protein content developed based on the SNP site, and the method is verified in a four-way recombinant inbred line material. The verification result shows that the SNP site discovered by the application is significantly related to the soybean seed protein content. The application provides a theoretical basis for molecular assisted breeding of the soybean protein content, and has important significance for cultivating new soybean varieties with high protein content.

Plants, plant parts, and a population of plants or plant parts comprising reduced BIG SEEDS (BS) activity, and compositions and methods of producing such plants, plant parts, and a population of plants and plant parts are provided. The plants, plant parts, or population can have a genetic mutation or a modification of the DNA methylation pattern that reduces the BIG SEEDS activity, which can be located at least partially in at least one BS gene or homolog or in its regulatory region. The plants, plant parts, and population can have increased organ size and / or protein or amino acid content. Also provided are seed, protein, and / or oil compositions and food and beverage products produced from the plants or plant parts provided herein.

The invention discloses an application of a soybean GmBBX8 gene in improving the protein content of soybean seeds, and the nucleotide sequence of the soybean GmBBX8 gene is shown as SEQ ID NO.1; the amino acid sequence of the soybean GmBBX8 protein is as shown in SEQ ID NO. 2. According to the invention, an overexpression transgenic soybean strain is created for the GmBBX8 gene, and the seed protein content of the GmBBX8 transgenic soybean is obviously improved compared with that of a wild type. Therefore, overexpression of the GmBBX8 gene in the soybean is one of effective modes for improving the protein content of the soybean seeds, and the method has important production and theoretical significance in breeding of soybean dominant varieties and development of germplasm resources.

The invention provides an application of a TaMYB46L-5A protein or a gene for coding the TaMYB46L-5A protein in regulating and controlling the protein content of plant seeds, and belongs to the technical field of molecular biology. The invention provides an application of a TaMYB46L-5A protein or a gene for coding the TaMYB46L-5A protein in at least one of regulation and control of protein content of plant seeds, creation of transgenic plants with dominant protein content and breeding of plant varieties with dominant protein content. The result of the embodiment of the invention shows that the seed protein content of a TaMYB46L-5A overexpression plant is obviously reduced, the seed protein content of a TaMYB46L-5A mutant plant is increased, and the contents of prolamin and glutenin are obviously increased. Therefore, the TaMYB46L-5A can negatively regulate and control the protein content of the plant seeds, and plays an important role in plant breeding.

The invention provides application of soybean GmSMS6 protein and a coding gene thereof in regulation and control of soybean grain quality traits. The genome sequence of the GmSMS6 is as shown in SEQ ID No. 1, and the coded amino acid sequence is as shown in SEQ ID No. 2. Gene editing is carried out on soybean Zp661 through a CRSIPR-Cas9 system, homozygous mutant plants Z1 and Z2 with the seed protein content obviously increased and the oil content obviously reduced compared with wild soybean Zp661 are obtained, and it is proved that soybean GmSMS6 has the function of regulating and controlling the quality of soybean seeds. The invention provides important gene resources and theoretical guidance for soybean molecular breeding and germplasm innovation.

The application relates to the technical field of plant genetic engineering, and discloses a TaPHYC-5A gene or biological material containing the gene and application of the TaPHYC-5A gene or the biological material in regulating the protein content of plant seeds. The gene TaPHYC-5A is cloned from a Chinese spring wheat genome, and the gene is overexpressed in Fielder wheat to obtain TaPHYC-5A gene overexpression plants. The detection result shows that the TaPHYC-5A gene overexpression can significantly increase the protein content in the wheat seeds, and the increase is more than 13% compared with a wild type control variety. Therefore, the TaPHYC-5A gene has the function of regulating the protein content of plant seeds, and can be applied to plant breeding or quality improvement.

The invention discloses an optimal buffer solution extraction test method for optimizing germinated walnut seed protein by a response surface method.The method comprises the steps that after seeds germinate, the amino acid composition and content of the walnut seeds in different germination periods are measured, and the optimal germination time is determined; then measuring the protein content and the antioxidant activity; and optimizing the optimal extraction condition by using a response surface method. According to the method, the current situation of walnut protein resource utilization and bean and cereal seed germination application research are combined, the protein content and amino acid composition and content change of germinated walnut seeds are analyzed, and the optimal germination time node is determined. According to the method, germinated walnut kernels are used as materials, a response surface optimization protein extraction test is utilized, antioxidant activity evaluation is combined, and an extraction and separation process for the antioxidant protein of the germinated walnut kernels is constructed, so that antioxidant protein components with high additional value are prepared, and accurate preparation of specific functional protein of the germinated walnut kernels is facilitated; a process and a scheme are provided for high-value utilization of subsequent walnut protein resources in the fields of food, health care products, daily chemicals and the like.

The invention relates to the technical field of meal replacement powder, provides meal replacement powder for improving intestinal environment and a preparation method, and aims to provide comprehensive nutrition and promote intestinal health through raw material combination in scientific proportion. The meal replacement powder consists of a component A (hemp seed protein powder and quinoa protein isolate), a component B (semen plantaginis husk powder and cactus fruit powder), a component C (oenothera biennis oil powder and sea buckthorn seed oil powder), a component D (agave syrup powder) and a component E (nattokinase and bacillus coagulans viable powder). The preparation method comprises the steps of low-temperature superfine grinding, multi-enzyme composite enzymolysis, embedding of probiotics and enzymes, fat microencapsulation, mixing and grinding, drying and packaging and the like. The meal replacement powder capable of improving the intestinal environment has positive effects in the aspects of promoting small intestine peristalsis, improving constipation, increasing the number of beneficial bacteria, reducing the number of harmful bacteria and maintaining intestinal microecological balance.

The invention discloses an extraction method of fructus cannabis protein and application of the fructus cannabis protein as an emulsifier for embedding fat-soluble functional components or application of the fructus cannabis protein as a protein carrier for loading the fat-soluble functional components. The extraction method comprises the following steps: crushing and sieving degreased fructus cannabis cake meal to obtain fructus cannabis cake meal powder; mixing the fructus cannabis cake meal with water, and stirring to obtain fructus cannabis meal slurry; adding a compound enzyme into the fructus cannabis pulp, and performing enzymolysis to obtain enzymatic hydrolysate; adjusting the pH value of the enzymatic hydrolysate to 8.0, stirring and mixing, then centrifuging, carrying out ultrafiltration on the supernate by using a membrane with the molecular weight cut-off of 10KDa, adjusting the pH value of the obtained cut-off liquid to 7.5, and then carrying out spray drying to obtain the fructus cannabis protein. Crude fibers, phytic acid and protein glutamine in the cake meal are selectively hydrolyzed through the compound enzyme, and the extraction rate, solubility, emulsibility and other characteristics of protein are remarkably improved. The extraction conditions are mild, the method is environment-friendly, and the obtained hemp seed protein can embed or load fat-soluble components.

The application discloses application of a rice nitrogen utilization gene NU5 in regulating growth of rice and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the rice nitrogen utilization gene NU5 is shown as SEQ ID NO. 1; the regulation of growth of rice refers to regulation of biomass of rice, regulation of seed quality of rice and regulation of protein content of seeds of rice. The application identifies an expression mode of the nitrogen utilization gene NU5 in rice, and obtains a loss-of-function mutant of the gene by using a gene editing technology. The loss-of-function mutant rice seedlings have reduced biomass, reduced 1000-grain weight, reduced protein content of seeds and reduced nitrogen utilization rate. The application provides a new gene resource and research basis for research on nitrogen metabolism of rice, research on growth regulation mechanism and regulation of seed quality.