47 results about "Seed protein" patented technology

The invention discloses a cross-platform soybean seed protein content estimation method based on a spectral time sequence. The method comprises the following steps: step 1, acquiring high spectral data of a soybean canopy; 2, collecting a soybean multispectral image; step 3, acquiring soybean physiological parameters and grain protein content data; step 4, obtaining soybean photosynthetic parameter data; step 5, calculating a vegetation index for estimation; step 6, constructing a vegetation index time sequence; 7, screening an optimal time window; 8, extracting dynamic characteristics; step 9, establishing a model, estimating the soybean protein content, and performing precision verification; and step 10, carrying out mobility analysis on the model from a near-ground canopy to a satellite platform. According to the method, a theoretical basis and a practical path are provided for realizing remote sensing-based soybean GPC accurate monitoring, wide application of the method on a satellite remote sensing platform can be promoted in the future, and high-precision, high-time-efficiency and large-scale soybean GPC estimation is realized.

The application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a molecular marker related to soybean seed protein content and application thereof, a nucleotide sequence of the molecular marker is shown as SEQ ID NO. 1, and is located in the interval of 32403844 bp-32403863 bp of the 16th chromosome of soybean. The QTL interval related to the soybean seed protein content identified by the application has a size of 0.076 Mb, the number of genes in the interval is small, and important foundation is provided for related gene mining. On the basis, one molecular marker related to the soybean seed protein content, namely SSR-16-1082, is developed in the interval, the molecular marker has high correlation degree with the soybean seed protein content, and important marker information is provided for molecular marker assisted selection breeding of the soybean seed protein content.

The application belongs to the technical field of protein extraction, and provides a method for improving water holding property and oil holding property of peony seed protein, the core of the method is that before the extraction of the peony seed protein, the peony seed is pretreated by the following method: the peony seed meal is subjected to steam explosion treatment under the condition of 0.4-1.2 Mpa and 60-150 s, preferably 0.8 Mpa and 100 s, to obtain the steam exploded peony seed, and the sample after the steam explosion is subjected to drying treatment; preferably heat pump drying. The peony seed protein prepared by the method has obviously improved water holding property, oil holding property, foaming property, foam stability, emulsifying property and emulsion stability; the valine is increased by 8.58%, the leucine is increased by 6.63%, and the tyrosine is increased by 10.65%; the extraction rate of the seed protein is 76.82%, and the extraction effect is excellent.

This invention discloses a hair repair composition for permed hair, comprising, by weight: 0.02-1.0 parts hydrolyzed plant protein, 0.1-2.0 parts biomimetic amino acid complex, 50-95 parts carrier, and 0.5-15 parts additives. The hydrolyzed plant protein is obtained from Moringa seed protein through multi-enzyme synergistic hydrolysis, with a molecular weight less than 3500 Daltons. The biomimetic amino acid complex includes at least wheat amino acids, soybean amino acids, arginine hydrochloride, serine, and threonine. Under weakly acidic conditions, this composition can simultaneously repair broken disulfide bonds, hydrogen bonds, and ionic bonds in hair. The hydrolyzed plant protein provides disulfide bond reconnection sites, and the biomimetic amino acid complex constructs a multi-point weak bond network through hydroxyl and ionic groups, achieving "triple bond repair" synergistically. This invention can significantly improve the mechanical strength, elasticity, and breakage resistance of damaged hair, with long-lasting, gentle, and safe repair effects, suitable for daily care of damaged hair after perming and dyeing.

The invention discloses China-hemp peptide multi-nutrient protein powder with a metabolism improving function, which comprises China-hemp seeds, soybean protein isolate, China-hemp seed protein, chickpea protein, pea protein, China-hemp peptide, rice protein, broad bean protein, mung bean protein and wheat hydrolyzed protein. Wherein the Chinese hemp seeds are 15-25 parts, the soybean protein isolate is 10-20 parts, the Chinese hemp seed protein is 5-15 parts, the chickpea protein is 8-12 parts, the pea protein is 8-12 parts, the Chinese hemp peptide is 8-15 parts, the rice protein is 5-10 parts, the broad bean protein is 5-10 parts, the mung bean protein is 5-10 parts, and the like. According to the present invention, besides the protein nutrition, the small molecule peptide and 10 other nutrients such as Omega3, gamma-linolenic acid, vitamin E and a variety of minerals are contained, such that the complete protein nutrition is provided, and the health-care food is rich in Omega-3 fatty acid, gamma-linolenic acid, vitamin E, potassium, magnesium, zinc and other minerals with different molecular magnitudes extracted from China-hemp seeds, the ten nutrients, a plurality of plant proteins and small molecule peptides have a synergistic effect to jointly form a basis for improving the metabolic function.

This invention relates to the soybean GmFAH gene and its application in improving seed protein content and branching number, belonging to the field of plant genetic engineering technology. The protein encoded by the soybean GmFAH gene is shown in SEQ ID NO.1. The encoding DNA sequence is shown in SEQ ID NO.2. The application of the soybean GmFAH gene in improving seed protein content and branching number is as follows: The method of this invention involves introducing the GmFAH gene DNA molecule into recipient soybeans to obtain transgenic soybeans. The protein content of the transgenic soybean seeds is significantly higher than that of the recipient soybeans, and the number of branches is significantly greater, while the grain weight and yield are not significantly different from those of the recipient soybeans. Using the method of this invention, the protein quality and plant type traits of soybean seeds can be improved, accelerating the efficiency of molecular breeding for superior soybean quality and ideal plant type.

The present application belongs to the technical field of food processing, and relates to a method for removing earthy smell of tilapia fillets. The method comprises the following steps: covering the surface of the tilapia fillets with pleurotus eryngii powder, and placing the tilapia fillets in a deodorizing and fresh-keeping liquid for 30-60 min; the deodorizing and fresh-keeping liquid is composed of ginger powder 15-20 parts, cannabis seed protein 5-10 parts, lysozyme 0.5-1 part, tea polyphenol 4-8 parts and water 60-100 parts in terms of mass fraction. The present application solves the technical problem of long-term maintenance of fresh fish flavor when removing the earthy smell of tilapia fillets.

The application discloses a SNP site related to soybean protein content and application thereof. The SNP site related to soybean protein content disclosed by the application is located at 35708082 of chromosome 19 of a soybean genome, and the polymorphism is C or A. The application also discloses a HRM melting curve method for identifying soybean protein content developed based on the SNP site, and the method is verified in a four-way recombinant inbred line material. The verification result shows that the SNP site discovered by the application is significantly related to the soybean seed protein content. The application provides a theoretical basis for molecular assisted breeding of the soybean protein content, and has important significance for cultivating new soybean varieties with high protein content.

The invention provides application of soybean GmSMS6 protein and a coding gene thereof in regulation and control of soybean grain quality traits. The genome sequence of the GmSMS6 is as shown in SEQ ID No. 1, and the coded amino acid sequence is as shown in SEQ ID No. 2. Gene editing is carried out on soybean Zp661 through a CRSIPR-Cas9 system, homozygous mutant plants Z1 and Z2 with the seed protein content obviously increased and the oil content obviously reduced compared with wild soybean Zp661 are obtained, and it is proved that soybean GmSMS6 has the function of regulating and controlling the quality of soybean seeds. The invention provides important gene resources and theoretical guidance for soybean molecular breeding and germplasm innovation.

The invention discloses an optimal buffer solution extraction test method for optimizing germinated walnut seed protein by a response surface method.The method comprises the steps that after seeds germinate, the amino acid composition and content of the walnut seeds in different germination periods are measured, and the optimal germination time is determined; then measuring the protein content and the antioxidant activity; and optimizing the optimal extraction condition by using a response surface method. According to the method, the current situation of walnut protein resource utilization and bean and cereal seed germination application research are combined, the protein content and amino acid composition and content change of germinated walnut seeds are analyzed, and the optimal germination time node is determined. According to the method, germinated walnut kernels are used as materials, a response surface optimization protein extraction test is utilized, antioxidant activity evaluation is combined, and an extraction and separation process for the antioxidant protein of the germinated walnut kernels is constructed, so that antioxidant protein components with high additional value are prepared, and accurate preparation of specific functional protein of the germinated walnut kernels is facilitated; a process and a scheme are provided for high-value utilization of subsequent walnut protein resources in the fields of food, health care products, daily chemicals and the like.

The invention belongs to the technical field of functional protein peptides, and particularly discloses a grass seed and cotton seed protein peptide-selenium nano-composite and a preparation method and application thereof.The method comprises the following steps that grass seeds and cotton seeds are extracted and degreased, and grass seed and cotton seed protein freeze-dried powder A is prepared; removing non-protein, fat and mineral substances from the cotton seed protein freeze-dried powder A to prepare cotton seed protein freeze-dried powder B; preparing the grass cottonseed protein peptide from the grass cottonseed protein freeze-dried powder B in a simulated gastrointestinal digestion environment; the grass seed and cotton seed protein peptide and sodium selenite are used as raw materials to prepare the grass seed and cotton seed protein peptide-selenium nano-composite. According to the grass seed and cotton seed protein peptide-selenium nano-composite and the preparation method and application thereof, grass seeds and cotton seeds are used as raw materials, and the prepared grass seed and cotton seed protein peptide-selenium nano-composite has the advantages of being high in absorption rate, high in safety and high in oxidation resistance; therefore, the organic selenium supplement can be widely applied to the field of future food or cosmetics as a high-efficiency dietary organic selenium supplement.

The invention discloses application of a soybean GmCDF3 gene in regulating and controlling the content of seed protein, amino acid and raffinose, and relates to application of soybean GmCDF3. According to the application of the soybean GmCDF3 gene in regulating and controlling the contents of seed protein, amino acid and raffinose, the application method is to culture plant seeds by using the soybean GmCDF3 gene, and further improve the plant quality. According to the invention, a GmCDF3 overexpression vector is constructed by utilizing a molecular means, and the soybean GmCDF3 gene is overexpressed in soybean through an agrobacterium-mediated genetic transformation technology, so that the content of 12 amino acids such as cysteine, leucine, lysine, phenylalanine and threonine in soybean seeds can be remarkably increased, and the yield of soybean seeds is increased. The protein content of the seeds is obviously improved, the oil content and the grain weight are not influenced, and the raffinose content is obviously reduced. Meanwhile, the soybean GmCDF3 gene is over-expressed in arabidopsis thaliana, and the protein content of arabidopsis thaliana seeds is also remarkably increased.

The invention discloses a lotus seed protein peptide preparation method and device capable of improving an antioxidant effect, and relates to the technical field of lotus seed protein peptide preparation. A driving mechanism is arranged in the device body, a temperature control mechanism and a stirring mechanism are connected to the left end of the driving mechanism, the temperature control mechanism comprises a peristaltic pump rod, a peristaltic pump shell is arranged on the outer side of the peristaltic pump rod, a liquid conveying hose is arranged between the peristaltic pump rod and the peristaltic pump shell, and a circulating pipe is connected to the left end of the liquid conveying hose; the stirring mechanism comprises a rotating shaft, a scraper is arranged at the bottom of the rotating shaft, an auger shaft is connected to the top of the scraper, a first stirring shaft is arranged on the outer side of the rotating shaft, and a limiting rod is arranged on the outer side of the rotating shaft and connected with a scraper and a second stirring shaft. The mixing mechanism is driven by the driving mechanism to operate, so that the rotating shaft drives the first stirring shaft and the second stirring shaft to mix the lotus seed powder raw material and the enzymatic hydrolysate in the mixing bin, the equipment can perform better mixing or enzyme deactivation work, and the situation that the yield of lotus seed protein peptide is reduced due to insufficient mixing is avoided.

This invention provides a sea buckthorn seed protein peptide, its preparation method, and its application, relating to the field of plant protein peptide extraction technology. The preparation method includes: pulverizing defatted sea buckthorn seed residue, performing ultrasonic-assisted extraction, adjusting the pH value to precipitate, and centrifuging to obtain a protein extract; adding a complex protease composed of alkaline protease, flavor protease, and neutral protease in a specific enzyme activity ratio for targeted enzymatic hydrolysis; purifying the hydrolysate sequentially through a 3kDa ultrafiltration membrane and a 1kDa nanofiltration membrane, collecting the permeate; adding an encapsulating agent and spray drying to obtain the final product. The sea buckthorn seed protein peptide prepared by this method has a molecular weight of less than 3kDa (≥85%), a purity of ≥90%, and exhibits excellent DPPH free radical scavenging ability and angiotensin-converting enzyme inhibitory activity. This invention achieves efficient extraction of sea buckthorn seed protein and targeted enrichment of active peptides. The product can be used as a functional food, health product, or pharmaceutical raw material for antioxidation and blood pressure reduction.

This invention discloses a skin-moisturizing short peptide and its applications, belonging to the field of bioactive short peptide technology. The amino acid sequence of the short peptide is shown in SEQ ID NO: 3, derived from lotus seed protein, and the molecular weight of the short peptide is less than 1000 Da. This invention precisely screened a novel moisturizing active short peptide, QEMGR (SEQ ID NO: 3), from lotus seed protein. It consists of only 5 amino acids, has a molecular weight of less than 1000 Da, and has the advantages of simple synthesis, low cost, and high transdermal absorption efficiency.

The invention discloses a protein TaEOG1 related to wheat grain quality regulation and control as well as a coding gene and application of the protein TaEOG1. The protein provided by the invention is the following (a) or (b): (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of the sequence 1, is related to plant seed quality regulation and is derived from the sequence 1. The TaEOG1 gene disclosed by the invention can control grain quality characters in wheat, namely homozygous mutation of the gene causes increase of protein content and precipitation value of wheat seeds. A new gene resource is provided for research on quality characters of plants, especially wheat grains, a wheat material with the TaEOG1 excellent allele type is selected for genetic improvement of wheat, and the gene plays an important role in application in the field of wheat breeding.

The invention belongs to the technical field of molecular breeding, and particularly relates to a rape SNP molecular marker combination, a 4K liquid phase breeding chip and application of the rape SNP molecular marker combination. The rape SNP molecular marker combination comprises 4387 significantly associated SNP loci aiming at seven key agronomic traits, namely the oil content, the oleic acid content, the protein content, the thousand seed weight, the thioglycoside content, the seed coat color and the single plant yield of the cabbage type rape, and specifically comprises 596 effective foreground loci and 3791 background loci. The rape SNP molecular marker combination can realize accurate detection and genotype identification aiming at seven key agronomic traits. A sample test verifies that the 4K liquid phase breeding chip has the advantages of high site pertinence, high detection accuracy and cost adaptation to breeding practice, can be used for molecular marker-assisted selection of rape varieties, remarkably shortens the cultivation period of high-quality and high-yield rape varieties, and is suitable for a large-scale rape breeding scene.

The invention discloses high-protein milk tea and a preparation method thereof, and the high-protein milk tea is prepared by adding water into compound protein solid powder, milk-based flavoring powder, black tea powder, maltodextrin, sodium carboxymethyl cellulose, stevioside and erythritol, blending, boiling, homogenizing, canning and sterilizing. Wherein the compound protein solid powder is prepared from whey protein, chia seed protein and soybean protein isolate through betaine modification treatment. According to the high-protein milk tea system disclosed by the invention, protein-polyphenol interaction and thermal aggregation phenomena are effectively inhibited through betaine modification, and precipitation is reduced; the algal oil microcapsule structure significantly improves the embedding rate and oxidation stability of grease, replaces traditional hydrogenated fat, and is more balanced in nutrition; meanwhile, all the components synergistically enhance solubility and dispersity, the final product has the characteristics of high protein content, good stability, excellent flavor, fine taste and the like, and the nutritional quality and sensory experience of the high-protein milk tea are comprehensively improved.

The application provides a polypeptide with antioxidant efficacy prepared from Zanthoxylum bungeanum Maxim seed protein, and the polypeptide is prepared by subjecting Zanthoxylum bungeanum Maxim seed protein to enzymolysis, centrifuging the enzymolysis solution, subjecting the centrifuged solution to ultrafiltration, and drying the filtrate. The polypeptide prepared by the application has good antioxidant efficacy after purification. The prepared polypeptide has high activity and can be used in the fields of medicines and functional foods.

The invention discloses a primer pair for identifying wheat with different seed protein contents under a low-fertility soil condition as well as an identification method and application of the primer pair. The primer pair comprises an upstream primer 1 and an upstream primer 2, wherein the nucleotide sequences of the upstream primer 1 and the upstream primer 2 are shown as SEQ ID No. 1 and SEQ ID No. 2; and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. Carrying out KASP reaction by using the primer pair and taking the extracted wheat genome DNA as a template, carrying out fluorescence signal reading on a product, and if the signal reading result is Fam allele, determining that the wheat is BB genotype, and if the signal reading result is Fam allele, determining that the wheat is BB genotype; if the result is Hex allele, the wheat is of a bb genotype, and the protein content of the wheat seeds of the BB genotype is greater than that of the wheat seeds of the bb genotype. The primer pair disclosed by the invention can be used for identifying wheat with different seed protein contents under a low-fertility soil condition, and has an important application value in wheat breeding and low-fertility land development and utilization.

This invention discloses a microemulsified plant soap containing moringa seed active ingredients and its preparation method. The plant soap comprises, by weight percentage, 40-70% soap base, 8-18% moringa seed oil, 0.5-3.0% moringa seed protein extract, 4-10% a non-soap-based surfactant system, 5-15% polyol, and the balance being water. Moringa seed oil is rich in oleic acid and has a low peroxide value, thus delaying oil auto-oxidation. The moringa seed protein extract is rich in small molecule peptides, which can form a stable protective layer at the oil-water interface, improving the stability of oil droplets. This microemulsified plant soap exhibits strong antioxidant properties, long-lasting effect, and skin-friendly properties.

This invention belongs to the field of whole grain deep processing technology, and relates to a high-resistant starch whole grain powder and its preparation method. The raw materials of the high-resistant starch whole grain powder include the following components by weight: chickpeas 25-45 wt%, quinoa 20-30 wt%, brown rice 10-20 wt%, pea protein isolate 10-20 wt%, hemp seed protein 1-10 wt%, fructooligosaccharides 1-5 wt%, and compound nutrients 0.5-4 wt%. The high-resistant starch whole grain powder has a resistant starch content ≥35 (g / 100g starch), a GI value ≤55, and a powder particle size ≤100μm. This invention significantly increases the resistant starch content of the obtained whole grain to over 35% through the synergistic effects of germination activating endogenous enzymes, fermentation promoting starch molecule rearrangement, and aging-induced retrogradation. The product has a GI value ≤55, a natural fermented aroma, and a fine, easily reconstituted texture.

The invention discloses hemp seed protein fermented milk rich in gamma-aminobutyric acid as well as a preparation method and application of the hemp seed protein fermented milk, and belongs to the technical field of functional foods. The preparation method comprises the following steps: boiling hemp seeds, pulping, filtering, blending with auxiliary materials such as cane sugar, unsalted butter and polydextrose, homogenizing, sterilizing, inoculating a mixed strain consisting of bifidobacterium lactis, lactobacillus acidophilus, lactobacillus mucus fermentation (a special strain capable of removing beany flavor in the hemp seeds) and lactobacillus plantarum (a strain capable of producing gamma-aminobutyric acid at high yield), and fermenting for 2-3 hours to obtain the health-care product. And adding sodium glutamate for fermentation, and finally refrigerating and after-ripening. The cannabis seed protein fermented milk prepared by a specific process and strain combination provided by the invention has unique fresh flavor of cannabis seeds, and is smooth in mouth feel and fresh and cool in taste; animal experiments prove that the fermented milk has remarkable functions of improving sleep, relieving anxiety, regulating neurotransmitter in brain and remodeling intestinal flora, and a new way is provided for developing plant-based fermented products with specific health-care effects.

The invention discloses a production method of spina date seed protein gel, and belongs to the technical field of food processing, the spina date seed protein gel is prepared by taking medicinal and edible spina date seed as a raw material, performing degreasing, alkali extraction and acid precipitation to obtain spina date seed protein isolate, and performing precursor solution preparation, compound probiotic fermentation and post-treatment gelling. The compound probiotic leavening agent is formed by compounding lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, lactobacillus plantarum and lactobacillus casei according to the equal proportion, the inoculation amount is 0.1 g / 100 mL precursor solution, constant-temperature fermentation is carried out at 42 DEG C for 10-20 h, and the preferable fermentation time is 15 h. A standardized spina date seed protein isolate extraction process is established, the screened compound probiotic leavening agent is high in adaptability, and cooperative regulation and control of the gel water-holding capacity and strength are achieved by regulating and controlling the fermentation time; the prepared gel is in a non-transparent solid state, does not have dehydration shrinkage, has the water-holding capacity of more than or equal to 70%, has the gel strength of more than or equal to 18g, has a uniform and porous three-dimensional network structure as a microstructure, and has excellent rheological properties; the process is green and safe, the operation is simple, industrial amplification can be realized, and the product has both nutrition and probiotic functions, can be directly developed into products such as plant-based yoghourt and the like, can also be applied as a food gel, realizes high-value utilization of the spina date seed resource, and has a good industrial prospect.

This invention belongs to the field of molecular biology and genetic breeding technology, and discloses a QTL related to soybean seed protein content. qPro12 Tightly linked molecular markers and their applications. A genome-wide association study (GWAS) was used to identify a seed protein content QTL on soybean chromosome 12. qPro12 The significantly associated SNP is located at position 7,757,075 on chromosome 12 of the reference genome Glycine_max_v2.1, and can explain 0.51% of the phenotypic variation in the associated population. PARMS markers developed using this SNP were used to detect genotyping in 256 soybean varieties, demonstrating clear typing and ease of operation, making it suitable for molecular breeding of soybean grain protein content.

The present invention relates to the use of a protein extract of ungerminated, de-oiled seeds of Moringa oleifera as an anti-pollinator.The present invention further relates to a protein extract of ungerminated, de-oiled seeds of Moringa oleifera for use in the treatment and / or prevention and / or reduction of the manifestations of pollen allergy and / or the symptoms and / or signs associated with pollen allergy.

The invention discloses a method for extracting polypeptide from stauntonia chinensis seeds, which comprises the following steps: S1, raw material pretreatment: crushing and sieving the stauntonia chinensis seeds, and degreasing with a degreasing solvent to obtain degreased stauntonia chinensis seed powder; s2, protein extraction: mixing the degreased stauntonia chinensis seed powder with deionized water, adjusting the pH value to an alkaline condition to extract protein, and performing centrifugation, acid precipitation, centrifugation and drying to obtain stauntonia chinensis seed protein powder; s3, polypeptide preparation: mixing the stauntonia chinensis seed protein powder with deionized water, adding protease to perform enzymolysis reaction, after enzymolysis is finished, performing enzyme deactivation and centrifugation, and collecting supernate to obtain the stauntonia chinensis seed polypeptide. Protein in the stauntonia chinensis seeds is extracted by adopting an alkali-solution and acid-isolation method, and the stauntonia chinensis seed protein is converted into active polypeptide by adopting an enzymolysis technology.

Methods of producing a mutated wheat plant having an increased wheat yield content, an increased seed protein content, an increased biomass content, or a combination thereof, are also disclosed comprising: introducing at least one TB1 null allele mutation, at least one Rht semi-dwarfing allele mutation, or a combination thereof to a wheat plant seed to produce a first mutated wheat plant seed, wherein the mutated wheat plant has a modified height as compared to a nonmutated wheat plant.

The application discloses a method for regulating rice growth by using NU1-H and application thereof, and belongs to the technical field of genetic engineering. The application provides a method for regulating rice growth, which comprises the step of introducing NU1-H into the rice, wherein the nucleotide sequence of the NU1-H is shown as SEQ ID NO. 1. The regulation of the rice growth comprises regulation of nitrogen utilization rate of the rice, regulation of seed protein content of the rice and regulation of yield traits of the rice. The application provides that the NU1-H plays a key role in regulation of rice growth, including nitrogen utilization rate of the rice, yield of the rice, rice protein content and the like, and provides a new gene resource and research basis for research on nitrogen metabolism of the rice.