The invention discloses a camellia seed protein hair conditioner and a preparation method. The camellia seed protein hair conditioner is prepared from camellia seed protein extracting solution, a main surfactant, an auxiliary surfactant, a humectant, a fatting agent, a thickening agent, a modifier and a preservative. The preparation method comprises the following steps: mixing the humectant, a chelating agent, the modifier and the camellia seed protein extracting solution, thus obtaining an A phase, carrying out uniform mixing, and heating the A phase to 80 DEG C; uniformly mixing the main surfactant, the auxiliary surfactant, the fatting agent and the thickening agent, thus obtaining a B phase, heating the B phase to 80 DEG C; keeping at 80 DEG C, adding the A phase to the B phase, carrying out homogenizing for 2min at the speed of 8000rpm, carrying out emulsification till the complete state is reached, cooling to 40 DEG C, adding with the preservative, carrying out stirring at 1000rpm, and carrying out discharging after 10min. The camellia seed protein hair conditioner is yellow paste, has the efficacies of caring and nourishing the hair, preserving the moisture, and conditioning and moistening the hair, is safe without irritation, can be used for a long time, has relatively good rheological property, can adapt to the majority of packing materials, and is beneficial for the commercial production of products.