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Method for extracting oil camellia protein from camellia seed cake

A technology of camellia oleifera seed protein and camellia oleifera seed meal, which is applied in the fields of peptide preparation, chemical instruments and methods, organic chemistry, etc.

Inactive Publication Date: 2013-03-13
安徽新世界绿洲茶油有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no research reports on Camellia oleifera polypeptide at home and abroad

Method used

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Embodiment Construction

[0015] Crush the camellia seed protein and pass it through an 80-mesh sieve. According to the substrate concentration of 2.9%, add water and mix it. Heat denaturation treatment at 90°C for 20 minutes, then cool down to below 60°C. First adjust the pH value to 5.5, and then adjust the pH value 8.5, let it stand for 4 hours; then adjust the pH value to 7.0, add 4364.1 u of neutral protease and 5000 u of papain per gram of substrate, stir and enzymatically hydrolyze at 53.4°C for 3h, after enzymatic hydrolysis, the temperature Rise to 85°C to inactivate the enzyme for 6 minutes, then centrifuge with a centrifuge at a speed of 4000r / min for 15 minutes, and take the supernatant; the supernatant is desalted with a resin column, filtered to obtain a filtrate; the obtained filtrate is chromatographed on a SephadexG-25 column, Using distilled water as the eluent at an elution rate of 0.3ml / min to obtain a chromatographic solution; then centrifuging the chromatographic solution; taking...

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Abstract

The invention provides a method for extracting oil camellia seed polypepetide from oil camellia seed protein. The method sequentially comprises the following steps of preparing the oil camellia seed protein, crushing, screening, adding water to obtain suspension, carrying out heat denaturation treatment, regulating pH value, carrying out enzymolysis with compound enzyme, carrying out enzyme deactivation, centrifuging, extracting supernatant, desalting with resin, carrying out SephadexG-50 column chromatography, centrifuging, extracting the supernatant, and carrying out freeze drying to obtain the oil camellia seed polypepetide. By adopting the method for extracting the oil camellia seed polypepetide from the oil camellia seed protein, disclosed by the invention, the enzymolysis time is shortened, the energy consumption is reduced, the extraction rate of the oil camellia seed polypepetide is also improved, and the purposes of turning waste into wealth and having low energy consumption and low pollution are achieved.

Description

technical field [0001] The invention relates to the field of camellia seed processing, in particular to a method for extracting camellia oleifera protein from camellia oleifera seed dregs. Background technique [0002] Camellia oleifera is an important oil crop in my country. my country is rich in camellia oleifera resources, with an annual output of more than 1.2 million tons of camellia oleifera seeds. The oil-pressed cake of Camellia oleifera seed contains about 12% to 15% protein, and its utilization rate is quite low, resulting in a great waste of resources. The peptides and amino acids produced by the hydrolysis of plant protein under the condition of biological enzyme method have high nutritional value and functional properties, so this method has become a research hotspot. In addition to being easily digested and absorbed by the human body, the peptides produced by the enzymatic hydrolysis of many plant proteins also have strong anti-oxidation and cholesterol-lowe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/16
Inventor 王仁宽
Owner 安徽新世界绿洲茶油有限公司
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