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9855 results about "Column chromatography" patented technology

Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential adsorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions. The technique is widely applicable, as many different adsorbents (normal phase, reversed phase, or otherwise) can be used with a wide range of solvents. The technique can be used on scales from micrograms up to kilograms. The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. The latter prevents cross-contamination and stationary phase degradation due to recycling. Column chromatography can be done using gravity to move the solvent, or using compressed gas to push the solvent through the column.

Method for extracting cannabidiol from cannabis

The invention discloses a method for extracting cannabidiol from cannabis. The method comprises the following steps: smashing and drying extraction fractions of cannabis to obtain medicine material powder; extracting the medicine material powder with 30% to 100% (V / V) of ethanol to obtain an extracting solution; concentrating the extracting solution to obtain an extract; carrying out water precipitation on the extract, and removing impurities to obtain a water precipitation solution; centrifuging the water precipitation solution and adding 10% to 100% (V / V) of ethanol into an obtained precipitate, and dissolving the precipitate to obtain an alcoholic solution of the precipitate; carrying out column chromatography on the alcoholic solution of the precipitate; eluting an obtained eluant after the concentration column chromatography, and adding ethanol for supersaturated dissolution to obtain a crystal substance; adding the crystal substance into purified water or ethanol for washing to obtain a primary product; blending the primary product with purified water, and drying to obtain the cannabidiol. Only highly safe ethanol is adopted as a solvent, the purity of the cannabidiol in a finished product is improved, and the psychotoxic component of tetrahydrocannabinol is also removed, so that the product safety is improved, and the method is applicable for industrial production.
Owner:YUNNAN HANSU BIO TECH CO LTD

Method for extracting cannabidiol from industrial hemp floral leaves

The invention discloses a method for extracting cannabidiol from industrial hemp floral leaves. The method comprises the following steps: grinding and drying floral leaves of industrial hemp, thereby obtaining medicinal powder; extracting the medicinal powder by adopting 30-100v% of ethanol, thereby obtaining extracting solution; concentrating the extracting solution to obtain extract; performing water precipitation on the extract, removing the impurities, thereby obtaining water precipitation solution; centrifuging the water precipitation solution, adding 10-100v% of ethanol into the obtained precipitate, dissolving to obtain an alcoholic solution of the precipitate; performing column chromatography on the alcoholic solution of the precipitate; concentrating the eluent obtained by eluting after column chromatography, adding ethanol to perform supersaturation dissolving, thereby obtaining a crystal; adding the crystal into purified water or ethanol for washing, thereby obtaining a primary product; uniformly mixing the primary product by using the purified water, drying, thereby obtaining the cannabidiol. According to the method disclosed by the invention, the highly safe ethanol serves as a solvent, so that the purity of the cannabidiol in the finished product is improved, a psychotoxic component, namely tetrahydrocannabinol, is removed, the product safety is improved, and the method is suitable for industrial production.
Owner:YUNNAN HANSU BIO TECH CO LTD

Method for extracting high-purity squalene by taking olive oil as raw material

The invention relates to a method for extracting high-purity squalene by taking olive oil as a raw material. A technological route formed by adopting a secondary molecular distillation and silica gel column chromatography is as follows: an olive oil unsaponifiable substance is taken, squalene is separated and purified by using two stages of molecular distillation, primary molecular distillation is carried out under the conditions of the evaporation surface temperature of 100 to 200 DEG C, the systemic pressure of 0.001 to 0.01mbar and the scraping film rotor speed of 150 to 300rpm, and secondary molecular distillation is carried out to the obtained distillate; the secondary molecular distillation is carried out under the conditions of the evaporation surface temperature of 150 to 300 DEG C, the systemic pressure of 0.001 to 0.01mbar and the scraping film rotor speed of 200 to 350rpm, and the obtained distillate is a squalene crude product; ethyl acetate-normal hexane with different concentrations is used as a mobile phase to carry out gradient elution, the obtained eluent is collected according to time and a solvent is evaporated, the same fractions are merged through chromatographic detection, and the high-purity squalene can be obtained, wherein the content of the raw material olive oil of the squalene is enhanced from 3.6% to about 98%; and especially, by considering the requirement of industrialized production to select an extraction condition especially, the large-scale production of the squalene taking the olive oil as the raw material can be realized.
Owner:JIANGSU ZODIAC MARINE BIOTECH

Production technique of andrographolide and neoandrographolide, dehydroanddrographolide, oxyandrographolide

The invention discloses a production technique of andrographolide and neoandrographolide, dehydroanddrographolide and oxyandrographolide; the technique comprises the following steps of: firstly preparing stem and leaf extract of andrographis paniculata, removing fat-soluble impurities such as chlorophyll and the like with petroleum ether, and then hot-melting the extract in lower alcohol or aqueous lower alcohol, conducting reflux and decolorization with active carbon, separating out a majority of andrographolide crystals, then removing flavonoid through an alumina column or alkali cleaning, obtaining the andrographolide, cold-melting the andrographolide with trichloromethane or dichloromethane for 2 to 4 times, filtering and obtaining two parts of filtrate and insoluble substances, concentrating the filtrate, then conducting solvent crystallization and recrystallization or column chromatography for separation, and finally, respectively obtaining dehydroanddrographolide and pure product of andrographolide; the insoluble substances go through solvent crystallization and recrystallization or column chromatography for separation to obtain the neoandrographolide and pure product of andrographolide. The technique has simple production equipment, simplified routes and easy operation, can realize industrialized batch production; and the proportion of neoandrographolide, dehydroanddrographolide and oxyandrographolide in the obtained andrographolide is high, while the content of impurities is low. The obtained neoandrographolide, dehydroanddrographolide and oxyandrographolide all have monomer purity of higher than 98 percent, thus being capable of being used as chemical reference substance of the traditional Chinese medicine, or being applied as raw materials of medicine and chemical industry.
Owner:雷允上药业集团有限公司
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