1544 results about "Column chromatography" patented technology

The invention discloses an online component detection method and system based on a salvia miltiorrhiza extracting solution chromatography elution process, and relates to the technical field of traditional Chinese medicine quality control. The method comprises the following steps: carrying out an elution experiment on a salvia miltiorrhiza extracting solution based on a column chromatography technology to obtain a sample set; acquiring near infrared spectrum and ultraviolet visible spectrum data in the sample; matching a spectrum fusion model according to the type of the to-be-detected component, and constructing different spectrum fusion models based on different fusion strategies for the near infrared spectrum and the ultraviolet visible spectrum; and processing data of the near infrared spectrum and the ultraviolet visible spectrum by using different spectrum fusion models to obtain the content of the to-be-detected component. On the basis of NIRS and UV-Vis technologies, online detection and analysis are carried out in the elution process of the salvia miltiorrhiza extracting solution by adopting a spectrum fusion and water spectrum omics method, and dynamic tracking and intelligent terminal point judgment of the salvia miltiorrhiza water-soluble active ingredients are realized.

The invention discloses hawthorn leaf pectic polysaccharide with lipid-lowering activity and a preparation method and application thereof, and belongs to a preparation method of plant polysaccharide, the preparation method comprises the following steps: refluxing hawthorn leaves with ethanol, discarding monosaccharide and small molecule compounds, refluxing with hot water to extract medicinal materials, removing protein through an enzymolysis method and a Sevage reagent method, decolorizing with polyamide resin, and drying to obtain the hawthorn leaf pectic polysaccharide with lipid-lowering activity. The crude polysaccharide is separated through DEAE-52 column chromatography, and finally, the crude polysaccharide is further purified through Sephadex G-100, so that the uniform purified polysaccharide is obtained. Tests on pancreatic lipase inhibition, taurocholate binding capacity and glycocholate binding rate show that the compound has remarkable lipid-lowering activity and is expected to become a potential natural lipid-lowering preparation.

The invention provides a preparation method of non-denatured type II collagen capable of increasing bone mineral density and improving bone elasticity, and belongs to the technical field of biologication.The preparation method comprises the steps that an EDTA-citric acid buffer solution containing beta-mercaptoethanol is adopted for decalcifying cartilage, a supercritical CO2 extraction technology is adopted for degreasing, and the collagen is obtained; alkaline protease and collagenase II are adopted for substrate directional enzymolysis and gradient digestion; then the non-denatured type II collagen is separated by adopting three-phase centrifugal separation, and the non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained by adopting bionic H column chromatography purification.

The invention relates to the technical field of ferula asafoetida endophytic fungi separation and purification, in particular to alkaloid compounds in ferula asafoetida endophytic fungi as well as a preparation method and application of the alkaloid compounds in the ferula asafoetida endophytic fungi. And extracting with dichloromethane to obtain dichloromethane part extract, and purifying and separating the dichloromethane part extract by adopting silica gel column chromatography and high performance liquid chromatography to obtain the alkaloid compounds in the endophytic fungi of ferula sinkiangensis. The alkaloid compound in the ferula sinkiangensis endophytic fungi is disclosed for the first time, the compound is subjected to an in-vitro anti-tumor pharmacodynamic experiment, and according to the experiment result, the alkaloid compound has a certain inhibition effect on MCF-7 cells; therefore, the alkaloid compound can be used for preparing the medicine for preventing and treating the breast cancer, and a new medicine is provided for preventing and treating the breast cancer.

The invention provides a separation and purification method of urokinase, and relates to the technical field of separation and extraction. The separation and purification method comprises the following steps: S1, regulating the pH value of male urine to 8.5, standing, and taking supernate; s2, mixing the supernate with an adsorption material, and eluting with an eluent to obtain a urokinase crude product solution; the adsorption material is a core-shell silica gel polymer, Fe3O4atSiO2-NH2 is taken as a core, and polystyrene is taken as a shell; the eluent is a sodium citrate buffer solution with the concentration of 0.5 to 0.15 M, acetonitrile with the concentration of 1 to 5 weight percent and EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 0.5 to 1.5 mM; and S3, carrying out anion exchange column chromatography on the urokinase crude product solution, eluting with an eluent, and precipitating to obtain the urokinase. The urokinase prepared by the method has the advantages of high polymer urokinase content, high recovery rate and high activity yield.

The invention belongs to the field of natural product extraction, and relates to a method for extracting and separating flavonoid compounds from Artemisia chrysantha, which comprises the following steps: carrying out reflux extraction on the dry overground part of Artemisia chrysantha by using 70% ethanol, loading to a balanced D101 macroporous adsorption resin column, sequentially eluting by using water, a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution, and collecting the eluent. Four fractions from Fr. I to Fr. IV are obtained; respectively carrying out polyamide column chromatography separation on Fr.II to Fr.IV, and sequentially eluting with a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution to respectively obtain three fractions; and further purifying the Fr.II-1, the Fr.II-2, the Fr.III-1, the Fr.III-2, the Fr.III-1 and the Fr.III-3 by virtue of a Sephadex LH-20 column, so as to finally obtain seven flavonoid compounds. According to the method, only the ethanol water low-toxicity solvent is used for extraction and preparation, organic solvents with high toxicity are not used, the method is green and environment-friendly, the extraction efficiency is high, the number of extracted compounds is large, chemical components of the artemisia europaea medicinal material are enriched, the resource source of active components is widened, and a foundation is laid for follow-up research.

The invention discloses an efficient separation and purification method of MLnM type medium-chain and long-chain triglyceride, and belongs to the technical field of separation and purification. The efficient separation and purification method comprises the following steps: S1, preparing a crude product; s2, mixing the crude product with an adsorbent for static adsorption; and S3, performing column chromatography elution on the static adsorption product, and performing dynamic chromatography to separate the MLnM type triglyceride. According to the efficient separation and purification method of the MLnM type medium-long-chain triglyceride, a neutral aluminum oxide static adsorption and dynamic column chromatography coupling process is adopted, the purity of the MLnM type triglyceride can be greatly improved and is far higher than that of other adsorbents, the whole process steps are clear, the steps of raw material preparation, static adsorption, dynamic chromatography and the like are included, and the method is suitable for industrial production. The operation process is easy for standardized and large-scale production, and does not need to depend on complex equipment such as molecular distillation, supercritical CO2 extraction and the like.

The invention discloses a method for preparing various bioactive peptides based on bean dreg fermentation, which comprises polypeptides with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and belongs to the field of microbial biological fermentation. Bacillus amyloliquefaciens sourced from fermented soybeans is used for fermentation, and a mixed product with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity is obtained. The invention also provides a method for purifying the polypeptide with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, which is characterized in that the mixed product is purified by combined column chromatography, the combined column chromatography comprises cation exchange resin LXP-01, macroporous adsorption resin HP20, weak base anion exchange resin D941, silica gel resin and Sephadex LH-20. The invention also discloses seven bioactive peptides with biological activity, the amino acid sequences are CKLLL, APGYSC, FPKYG, FPGPLV, FPRSH, FMAV and SVCC, and the bioactive peptides have good alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and can be used for producing functional foods, health care products, cosmetics and the like for assisting in reducing blood sugar, reducing blood pressure, resisting oxidation and the like.

The invention relates to the technical field of extraction and separation of compounds in traditional Chinese medicinal materials, in particular to a method for simultaneously separating and purifying three pentacyclic triterpenoids from a tripterygium hypoglaucum root medicinal material. Three pentacyclic triterpenoid monomers are obtained through the steps of smashing, reflux extraction, normal-phase silica gel chromatography, reversed-phase high-pressure column chromatography, recrystallization and the like, the method is high in extraction efficiency and small in reagent dosage, the three pentacyclic triterpenoid compounds can be obtained at the same time, and the obtained compounds are high in purity and yield, have high social and economic value and are suitable for industrial production. The purity of the obtained plasmin, tripterine and demethylzeylasteral is high, and a foundation is laid for the preparation and production of reference substances of triterpenoid components in the roots of pyracantha fortunei, the subsequent pharmacological activity and the development of new immune drugs.

The invention discloses a novel benzofuran component in eupatorium chinense and application of the benzofuran component in anti-inflammatory activity. The obtained compound is separated from an eupatorium chinense extract. The eupatorium chinense root extract is separated by using an extraction method, macroporous adsorption resin impurity removal, Sephadex LH-20 gel column chromatography, high performance liquid chromatography and other methods. According to the present invention, all the separated compounds and the analogues thereof have good inhibition activity on target cyclooxygenase-2 of inflammation-related diseases, and have good inhibition activity on COX-2 enzyme, and the molecular docking experiment results show that the compounds and the target proteins have good binding energy. Therefore, the compound can be used for preparing drugs for treating inflammatory related diseases, or anti-inflammatory and analgesic drugs, or tumor drugs by inhibiting COX-2 enzyme, such as drugs for rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, Alzheimer's disease and the like, and related natural lead compounds are provided.

The invention discloses a method for extracting nobiletin from citrus peel, which comprises the following steps: S1, crushing dried citrus peel, adding an alcohol-water solution into the citrus peel powder, heating and extracting to obtain a citrus peel extracting solution; s2, centrifuging the citrus peel extracting solution, collecting supernate, and filtering to obtain filtrate; s3, adding an adsorbent into the filtrate, stirring, standing, filtering, concentrating the filtrate, and recrystallizing to obtain nobiletin with the purity higher than 98%; the adsorbent is selected from at least one of diatomite, attapulgite and porcellanite. According to the method, the alcohol-water solution is added to extract the citrus peel, the adsorbent is added to adsorb the citrus peel extracting solution, high-purity nobiletin can be extracted from the citrus peel, meanwhile, the adsorbent is added into the extracting solution, the extraction rate of the nobiletin can be increased, and the method has the advantages of being easy to operate, low in cost and the like. Expensive production equipment or column chromatography is not needed, and the like.

The invention discloses an extraction method of coriolus versicolor polysaccharide. The method comprises the steps of crude extraction of the coriolus versicolor polysaccharide and purification of the coriolus versicolor polysaccharide. The crude extraction of coriolus versicolor polysaccharide comprises the following steps: water extraction and alcohol precipitation, H2O2 decoloration and freeze-drying; the purification of the coriolus versicolor polysaccharide comprises the following steps: carrying out anion exchange column chromatography on a coriolus versicolor polysaccharide crude product obtained by crude extraction of the coriolus versicolor polysaccharide, and carrying out ultrafiltration separation and purification to obtain the refined coriolus versicolor polysaccharide. The coriolus versicolor polysaccharide with the polysaccharide content of 80.18% and the protein content of 5.9% can be obtained through the preparation method. HPGPC analysis shows that the coriolus versicolor polysaccharide is uniform in molecular weight distribution, and the relative molecular weight Mw is about 17478 Da. The coriolus versicolor polysaccharide plays a role in treating the NAFLD by regulating intestinal flora and influencing bile acid metabolism, a theoretical basis is provided for treating the NAFLD by the coriolus versicolor polysaccharide, and a direction is broadened for NAFLD medicine development.

The invention relates to the technical field of photo-thermal therapy, in particular to a monomolecular photo-thermal agent with high photo-thermal conversion efficiency and a preparation method and application of the monomolecular photo-thermal agent. The preparation method comprises the following steps: S1, synthesis of a compound X1: under the protection of argon, dissolving 2, 4-dimethyl pyrrole in dichloromethane, stirring in an ice bath, adding trifluoroacetic anhydride, heating to room temperature after stirring, extracting after quenching reaction, combining organic phases, drying, filtering, concentrating under reduced pressure and purifying by silica gel column chromatography to obtain the compound X1. The prepared monomolecular photothermal agent with the high photothermal conversion efficiency is high in water solubility, has the high photothermal conversion efficiency in an aqueous solution and is high in light stability, the immune escape phenomenon possibly caused by introduction of a high polymer material is avoided, and the technical problem that an existing photothermal agent is difficult to achieve the high photothermal conversion efficiency in the aqueous solution is solved.

The invention provides novel application and a method of asiaticoside. The method comprises the following steps: S1, extraction: taking a centella asiatica medicinal material, performing coarse crushing, adding ethanol, performing reflux extraction, performing filtration, and combining filtrate for standby application; s2, concentrating: concentrating the ethanol extracting solution under reduced pressure until no alcohol smell exists, centrifuging, and reserving supernate for later use; s3, carrying out D101 column chromatography; s4, alumina column chromatography; s5, performing concentration; s6, drying; s7, dissolving with methanol, adding water, standing, and monitoring clear liquid and crystallization conditions in a liquid phase; s8, filtering after crystallization is finished, washing with ethanol, drying crystals under the conditions that the vacuum degree is-0.06 to-0.08 mpa and the temperature is 60-70 DEG C, and crushing and sieving dry paste to obtain asiaticoside; specifically, the new application comprises reduction of uric acid (UA), urea (UREA), creatinine (CREZ), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and total cholesterol (CHOL) in serum of a hyperuricemia model SD rat, and reduction of damage of hyperuricemia to kidney. And the yield of asiaticoside extracted by the method is high and is about 84%.

The invention discloses a digestion-aiding highland barley fried malt polysaccharide as well as a preparation method and application thereof. The polysaccharide is extracted from highland barley through hot water extraction, ethanol precipitation, deproteinization, ion exchange column chromatography, gel column chromatography and dialysis processes. Animal experiments show that the polysaccharide can significantly improve the small intestine propulsion rate of mice and increase the weight increment, gastric juice amount, pepsin activity and pepsin discharge of rats, and the effect of the polysaccharide is superior to that of traditional roasted barley malt polysaccharide; the highland barley polysaccharide is superior to highland barley crude polysaccharide, highland barley polyphenol, highland barley lipid, highland barley protein and the like. The invention has the advantages of easily available raw materials and green process, is suitable for developing side-effect-free digestion-aiding functional foods such as tea bags, milk tea, yoghurt and biscuits, and has important market value.

The invention discloses a preparation method of a 6-alkyl aza-uracil compound, which comprises the following steps: in a nitrogen atmosphere solvent, irradiating a reaction mixture of an aza-uracil derivative and carboxylic acid active ester with visible light, adding a catalyst and stirring for reaction, and after the reaction is completed, performing column chromatography separation on the reaction mixed solution to obtain the 6-alkyl aza-uracil compound. The product 6-alkyl aza-uracil compound can be prepared. The synthesis method of the 6-alkyl aza-uracil compound provided by the invention does not need to use a photosensitizer and a metal catalyst, the reaction conditions are green and mild, and the operation is simple, convenient, rapid and efficient.

The present invention provides a method for preparing an organostannane compound catalyzed by diethylzinc, belonging to the technical field of preparing stannane compounds. The method can solve the problems urgently needed to be solved in the preparation method of such stannane compounds. The method comprises: mixing an acetylene compound, a tin hydrogen compound, and a diethylzinc catalyst under an inert atmosphere; reacting the reaction system at 60 to 70°C for 18 to 24 hours, then exposing the reaction system to air to terminate the reaction, and purifying the organostannane compound by column chromatography, wherein the inert atmosphere is nitrogen. The stannane compound is a vinyl stannane compound or an ethynyl stannane compound, and the acetylene compound is an aliphatic acetylene compound or an aromatic acetylene compound. The present invention solves the problems urgently needed to be solved in the preparation method of such organostannane compounds, such as the high cost of the catalyst used, the environmentally unfriendly nature of the catalyst, and the complex operation process.

The invention discloses a radix pseudostellariae extract as well as a preparation method and application thereof. The preparation method comprises the following steps: providing a radix pseudostellariae raw material; the radix pseudostellariae raw material is subjected to water extraction to prepare a water extract; eluting the water extract through macroporous adsorption resin column chromatography to prepare a first extract; and eluting the first extract through ion exchange resin column chromatography to prepare the radix pseudostellariae extract. By adopting a method of combining macroporous adsorption resin column chromatography and ion exchange resin column chromatography, the yield of the radix pseudostellariae extract is improved, particularly the polysaccharide content and the specific monosaccharide content and proportion in the radix pseudostellariae extract are improved, and the functional activity of the radix pseudostellariae extract is greatly improved. Meanwhile, the preparation process greatly reduces the use of organic solvents, is green and safe in production process, and has good use value in the fields of food, medicines and the like.

The utility model discloses a system for step-by-step separation and conversion of tar residues, which comprises an ultrasonic cleaning instrument, the liquid outlet end of the ultrasonic cleaning instrument is connected with a solid-liquid separator through a pipeline, the liquid outlet end of an upper liquid layer of the solid-liquid separator is connected with a delivery pump I through a pipeline, and the delivery pump I is connected with the liquid inlet end of a rotary evaporator I through a pipeline. Concentrated organic matters in the rotary evaporator I are conveyed into a vertically arranged chromatographic column through a pipeline, the lower end of the chromatographic column is connected with a sand core funnel, a collecting tank is placed below an outlet of the sand core funnel, the collecting tank is connected with a conveying pump V through a pipeline, and the conveying pump V is connected with a liquid inlet end of the rotary evaporator II through a pipeline. According to the method, high additional organic matters in the tar residues can be effectively extracted by adopting a mode of synergism of ultrasonic-assisted extraction and column chromatography separation, so that resource utilization of the tar residues is realized. The solvent and the eluent selected in the whole process can be recycled through a rotary evaporator, the operation cost is low, and near-zero emission can be realized.

The present invention relates to a preparation method of a wheat germ extract rich in spermidine. The method is characterized in that the method realizes the preparation of a wheat germ extract rich in spermidine from wheat germ by using macroporous adsorption resin combined with silica gel column chromatography technology. The raw material of the present invention is wheat germ for extraction, which is the raw material with the highest spermidine content in plants, providing the best choice for the preparation of a wheat germ extract rich in spermidine. And ethanol is used as the extraction solvent, which is simple, commonly used, easy to obtain and safe. Compared with other supercritical carbon dioxide, trichloroethanol, etc., the extraction cost is greatly reduced. The processing process of this method is simple, the preparation time is short, the extraction rate is high, and the thermal degradation of heat-sensitive components can be effectively avoided. The content of spermidine in the obtained wheat germ extract is 220 times that of wheat germ, which can effectively reduce the cost and increase the content of spermidine in the extract.

The invention belongs to the technical field of agricultural chemistry, and particularly relates to a naphthoquinone compound with tobacco black shank resisting activity as well as a preparation method and application of the naphthoquinone compound. The naphthoquinone compound is prepared by the following steps: by taking honeysuckle branches as raw materials, extracting with high-concentration methanol, high-concentration acetone / water or high-concentration ethanol / water, merging extracting solutions, filtering, and concentrating under reduced pressure to obtain extract; packing the extract by using a silica gel dry method and carrying out silica gel column chromatography; carrying out gradient elution by using a chloroform-acetone solution; and further separating and purifying the 7: 3 part of the eluent by high performance liquid chromatography to obtain the required naphthoquinone compound. The invention further discloses application of the compound, and activity tests show that the compound has a good inhibiting effect on tobacco black shank. The compound disclosed by the invention is novel in structure, has relatively good black shank resisting activity, can be used as a lead compound for resisting tobacco black shank, and is used for research and development of biological pesticides for preventing and treating tobacco black shank.

The invention discloses a preparation method and application of a benzofuran component in eupatorium chinense. The benzofuran component is separated from an eupatorium chinense extract. The eupatorium chinense root extract is separated by using an extraction method, macroporous adsorption resin impurity removal, HW-40F gel column chromatography, high performance liquid chromatography and other methods to obtain the benzofuran compound. According to the present invention, the inhibition activity of the separated compound on the diabetes-related target alpha-glucosidase is tested, the experimental result shows that the compound has good inhibition activity on the alpha-glucosidase, and the molecular docking experiment shows that the compound has good binding energy with the target protein alpha-glucosidase. Therefore, the compound can be used for preparing drugs for preventing, delaying or treating alpha-glucosidase diseases, especially type II diabetes and obesity, or can be used as a lead compound of the drugs.

The invention discloses application of plant-derived glyceroglycolipid in preparation of an anti-inflammatory product, inflammation is RAW264.7 cell inflammatory response induced by LPS (lipopolysaccharide), and the plant-derived glyceroglycolipid can resist inflammation by inhibiting expression of inflammatory factors. By combining column chromatography with chromatographic analysis, efficient extraction, precise separation and structural identification of the plant GL are realized, and important technical support is provided for research of plant lipids. The plant GL can significantly inhibit inflammatory response of RAW264.7 macrophages, including reduction of LPS-induced cellular morphological change, down-regulation of inflammatory factor mRNA expression and reduction of CD86 expression level. The invention provides a solid molecular level theoretical basis for the application of the plant glycerol glycolipid in the development of anti-inflammatory functional foods and medicines.

The invention discloses a preparation method of linolenic acid structural ester with synergistic biological activity, and relates to the technical field of linolenic acid structural ester preparation.The preparation method comprises the steps that specific lipase is prepared through rhizopus wei, and after an SBA-15 molecular sieve is treated, the lipase is fixed to prepare a composite catalyst; the preparation method comprises the following steps: after hydroxyl protection of quercetin, esterifying quercetin with EPA to obtain quercetin-EPA ester; esterifying glycerol and linolenic acid in the presence of a composite catalyst and microwaves, and purifying to obtain an intermediate product; carrying out microwave catalytic ester exchange on the intermediate product and quercetin-EPA ester in tert-butyl alcohol through a composite catalyst to obtain a crude product; and removing impurities from the crude product by a urea adduction method, and carrying out molecular distillation graded purification, column chromatography, deodorization and filtration to obtain a final product. According to the invention, linolenic acid, quercetin and EPA are combined through molecular design to form a compound structure ester with a multi-target effect, and the linolenic acid, quercetin and EPA achieve functional complementation through a synergistic effect, so that the biological activity of the compound structure ester is enhanced.

The invention relates to the technical field of polysaccharide preparation, in particular to mesona chinensis acidic polysaccharide and an extraction method thereof. The extraction method comprises the following steps: 1) crushing, degreasing and drying dried mesona chinensis, boiling and performing alkali extraction to obtain a mesona chinensis extracting solution; macroporous resin D-900 is adopted for adsorption to remove pigments, and crude mesona chinensis benth polysaccharide is obtained; carrying out primary purification by adopting DEAE-52 ion exchange column chromatography; and performing chromatographic purification through a Sephadex G-100 ion exchange column, collecting components, and performing freeze-drying to obtain the mesona chinensis benth acidic polysaccharide. The extraction method of the mesona chinensis benth acidic polysaccharide has the beneficial effects that the operation is simple, the cost is low, the characteristics of good decoloring effect and high polysaccharide preservation rate are also realized, and the biological activity of the mesona chinensis benth acidic polysaccharide can be guaranteed. Compared with other resins, the D-900 macroporous resin is used for selectively adsorbing melanin, the adsorption capacity of the D-900 macroporous resin to mesona melanin is remarkably higher than that of other resins, the adsorption rate of the D-900 macroporous resin to polysaccharide is lower than 2%, and the D-900 macroporous resin has the advantages of being good in decolorization effect and high in polysaccharide preservation rate.

The invention belongs to the technical field of rabies vaccines, and particularly relates to a purification method of a rabies virus inactivated vaccine, which comprises the following steps: step 1, carrying out passage by using a Walvax-2 cell strain through a cell factory method, and carrying out multiplication culture on the passage production cells in a microcarrier bioreactor; 2, cleaning the cells with a treating fluid, inoculating the cells with a rabies virus fixed strain, and preparing and culturing a rabies virus solution; step 3, carrying out ultrafiltration concentration on the rabies virus stock solution; step 4, purifying the rabies virus concentrated solution; step 5, inactivating the rabies virus; and 6, hydrolyzing to obtain the rabies vaccine stock solution. By adopting the composite Capto Core 700 column chromatography, impure proteins can be effectively removed, the recovery rate is high, the quality of the rabies vaccine obtained by adopting a mode of firstly purifying and then adopting beta-propiolactone inactivation is excellent, and the effect of the finally obtained human diploid cell rabies vaccine stock solution is good.

The invention discloses a preparation method of a tea leaf extract rich in tea aroma components, tea polyphenols and caffeine. The method comprises the following steps: firstly, extracting and separating aroma components in tea leaves by using a supercritical CO2 extraction technology, extracting residual residues by using ethanol, separating and enriching by using a normal-phase / reversed-phase column chromatography technology, simultaneously obtaining tea polyphenol and caffeine, and removing other ineffective components, thereby obtaining the tea leaf extract with relatively strong antioxidant, whitening and soothing effects; the color is appropriate, the fragrance is stronger, the stability is better, the activity is better, and the application range is wider; moreover, the whole extraction operation process is simple, and large-scale production is easy, so that the method can be widely applied to products such as cosmetics, shampoo, shower gel, facial cleanser, food and beverage, health care products and the like.

A method for preparing theasinensin A (TSA) by means of a low-temperature enzyme process. The method comprises using a plant polyphenol oxidase enzyme extract or polyphenol oxidase enzyme formulation as an enzyme source, using epigallocatechin gallate (EGCG) as a substrate, using as a reaction system a buffer solution having a certain pH value, and performing an enzymatic reaction at a low temperature and subsequent steps such as column chromatography, distillation and concentration, liquid phase preparation, and freeze drying, thereby synthesizing TSA in a directional enzymatic manner. The method achieves EGCG enzymatic oxidation at a low temperature and obtains dimeric TSA by means of a conversion reaction, laying a theoretical and practical basis for green, safe, efficient and large-scale industrial production and utilization of TSA.

The invention belongs to the technical field of phytochemistry, and particularly relates to a benzo [e] pyrrolo [1, 2-a] aza # imgabs0 # compound as well as a preparation method and application thereof. The preparation method comprises the following steps: fermenting cigar stem barks, drying the fermented cigar stem barks in the sun, crushing and sieving to obtain a material a; adding a first solvent into the material a for reflux extraction to obtain an extracting solution, concentrating the extracting solution, adding a second solvent for extraction, combining the extracted extraction phases, and performing vacuum concentration to obtain an extract b; carrying out silica gel column chromatography on the extract b, eluting with chloroform-acetone eluent, monitoring by TLC (Thin Layer Chromatography), and collecting the eluent; and purifying the eluent by using a sephadex column, and separating and purifying by using high performance liquid chromatography to obtain the benzo [e] pyrrolo [1, 2-a] aza # imgabs 1 # compound with tobacco black shank resisting activity. The compound is novel in structure, has relatively good black shank resistance activity, can be used as a lead compound for resisting tobacco black shank, and is used for research and development of biological pesticides for preventing and treating tobacco black shank.

The present invention provides a paroxysmal hemoglobinuria indication drug ipropam key intermediate synthesis method, and relates to the technical field of ipropam, the method comprises: taking p-bromobenzaldehyde as an initial raw material, adopting an S-configuration catalyst to obtain an intermediate 03, carrying out protection group removal on the intermediate 03, carrying out automatic ring closing to obtain an intermediate 04, and carrying out post-treatment to obtain the paroxysmal hemoglobinuria indication drug ipropam key intermediate. Reducing the intermediate 04 with sodium borohydride to obtain a single cis-configuration intermediate 05, then carrying out a chiral flip reaction and hydrolysis to remove p-nitrobenzoic acid, then carrying out an etherification reaction with diethyl sulfate to obtain an intermediate 08, reducing amide of the intermediate 08 with sodium borohydride and boron trifluoride diethyl etherate to obtain an intermediate 09, then carrying out an acetylation reaction to protect amino, and finally carrying out a reaction to obtain the chiral cis-configuration intermediate 03. Cuprous cyanide is subjected to a cyanation reaction and a hydrolytic esterification reaction to obtain the ipropam key intermediate TM. The method is low in raw material price, high in chiral control selectivity, easy in reaction condition control and low in equipment requirement, avoids column chromatography and other operations, and is more suitable for industrial production.