2436 results about "Column chromatography" patented technology

The invention relates to the technical field of medicine synthesis, and particularly discloses a preparation method of rosmeltirol. According to the preparation method of the rosmeltirol, provided by the invention, an existing production process of the rosmeltirol is adjusted, so that the total yield of a route is increased from 8.9% to 40% or above, the purity of the prepared rosmeltirol is greater than 99.9%, the content of the maximum single impurity in the product is less than 0.05%, and column chromatography purification is not adopted in the whole production process, so that the yield of the rosmeltirol is greatly improved. The method has the advantages of simple operation, mild production conditions, high safety, environmental protection, facilitation of realization of industrial production of the rosmeltirol, and high practical value, and the production cost and the production period are reduced.

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.

The invention discloses bullfrog skin peptide with DPP-IV inhibitory activity and a purification method and application thereof.The molecular weight of the bullfrog skin peptide is 681.40 Da, and the amino acid sequence of the bullfrog skin peptide is shown as SEQ ID NO.1. The bullfrog skin peptide is extracted through an enzymolysis means, then components containing peptide fragments are extracted through a column chromatography purification mode, finally the components are evaluated and screened, and the bullfrog skin peptide with the DPP-IV inhibitory activity is obtained. The purified bullfrog skin peptide with the DPP-IV inhibitory activity is obtained. The bullfrog skin peptide with the DPP-IV inhibitory activity has the advantages of being strong in DPP-IV enzyme inhibitory effect, safe and free of toxic and side effects, has the application potential of hypoglycemic peptides, is short in amino acid sequence and strong in operability, and has good social popularization and application value.

The invention relates to a glabridin purification process for low-glabridin, which comprises the following steps: removing glabridin in a glabridin product by using a metal organic framework material (MOFs); the purification process comprises the following steps: extracting glycyrrhiza glabra roots with ethanol or methanol, and filtering to remove medicine residues to obtain an extracting solution; concentrating the extracting solution under reduced pressure to a certain volume, and then adsorbing and removing the glabriol through a filler column filled with an MOFs material; or enabling the extract liquor in the purification process of the glabridin to flow through a filler column filled with the MOFs material to adsorb and remove the glabriol; or adding the MOFs material into other fillers in a glabridin column chromatography purification process to adsorb and remove the glabridin, so as to obtain a glabridin product with low glabridin content. According to the method disclosed by the invention, the glabriol in the glabridin is simply and effectively removed, the content of the glabriol in the prepared glabridin product is lower than 0.1%, the safety of the product is improved, and the method has a strong market competitive advantage. The adsorption material can be recycled after being heated and desorbed, and has potential application prospect and wide application value.

Disclosed in the present invention is a method for simultaneously preparing high-purity sphingomyelin and glycerol phosphocholine. The method comprises the following steps: 1) adding a degradation agent to a phospholipid mixture to degrade phospholipid, so as to obtain a sphingomyelin and glycerol phosphocholine mixture, and carrying out separation by means of a refining method to obtain a crude product; 2) carrying out column chromatography to obtain sphingomyelin having a purity higher than 98%; and 3) obtaining glycerol phosphocholine having a purity higher than 95% by means of a refining method; and 4) drying same to obtain a phospholipid satisfying the pharmaceutical standard. The solvent used in the present invention can be recycled and reused and is environmentally friendly, and the method has mild reaction conditions, good selectivity, low requirement for the raw material purity, and a relatively low production cost, and is beneficial to industrial mass production.

The invention discloses a preparation method of four new skeleton alkaloid compounds with anti-inflammatory effect in the root of Anachymin and the use of the anti-inflammatory activity thereof. The four new skeleton alkaloid compounds are extracted from the root (root of Anachymin) of Roman pyrethrum (Anacyclus pyrethrum (L.) DC.) by using an organic solvent, and then separated by two or three methods of normal phase silica gel column chromatography, reverse phase silica gel column chromatography and semi-preparative high performance liquid chromatography to obtain eight new skeleton alkaloid monomer compounds, and the structures thereof are identified by high-resolution mass spectrometry, nuclear magnetic resonance spectroscopy and X-ray single crystal diffraction. The invention also provides the NO inhibition effect of these compounds.

The invention discloses a polycyclic polyisopentenyl phloroglucinol compound as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The method comprises the following steps: by taking a dry overground part of hypericum monogynum as a raw material, performing percolation extraction and concentration to obtain an extract, suspending the extract in water to obtain a suspension, and performing extraction and concentration to obtain a petroleum ether part extract; the extract is taken and subjected to separation through a positive and negative phase silica gel column, MCI medium-pressure column chromatography and Sephadex LH-20 gel column chromatography and purification through high performance liquid chromatography, the polycyclic polyisopentenyl phloroglucinol compound is prepared, and the structure and configuration of the compound are determined by means of a spectrum analysis technology, quantum chemistry calculation, X-ray single crystal diffraction and the like. The compound can inhibit TNF-alpha protein to play an immunosuppressive role, and can be used as an immunosuppressive drug.

The invention discloses an online component detection method and system based on a salvia miltiorrhiza extracting solution chromatography elution process, and relates to the technical field of traditional Chinese medicine quality control. The method comprises the following steps: carrying out an elution experiment on a salvia miltiorrhiza extracting solution based on a column chromatography technology to obtain a sample set; acquiring near infrared spectrum and ultraviolet visible spectrum data in the sample; matching a spectrum fusion model according to the type of the to-be-detected component, and constructing different spectrum fusion models based on different fusion strategies for the near infrared spectrum and the ultraviolet visible spectrum; and processing data of the near infrared spectrum and the ultraviolet visible spectrum by using different spectrum fusion models to obtain the content of the to-be-detected component. On the basis of NIRS and UV-Vis technologies, online detection and analysis are carried out in the elution process of the salvia miltiorrhiza extracting solution by adopting a spectrum fusion and water spectrum omics method, and dynamic tracking and intelligent terminal point judgment of the salvia miltiorrhiza water-soluble active ingredients are realized.

The invention discloses hawthorn leaf pectic polysaccharide with lipid-lowering activity and a preparation method and application thereof, and belongs to a preparation method of plant polysaccharide, the preparation method comprises the following steps: refluxing hawthorn leaves with ethanol, discarding monosaccharide and small molecule compounds, refluxing with hot water to extract medicinal materials, removing protein through an enzymolysis method and a Sevage reagent method, decolorizing with polyamide resin, and drying to obtain the hawthorn leaf pectic polysaccharide with lipid-lowering activity. The crude polysaccharide is separated through DEAE-52 column chromatography, and finally, the crude polysaccharide is further purified through Sephadex G-100, so that the uniform purified polysaccharide is obtained. Tests on pancreatic lipase inhibition, taurocholate binding capacity and glycocholate binding rate show that the compound has remarkable lipid-lowering activity and is expected to become a potential natural lipid-lowering preparation.

The invention provides a preparation method of non-denatured type II collagen capable of increasing bone mineral density and improving bone elasticity, and belongs to the technical field of biologication.The preparation method comprises the steps that an EDTA-citric acid buffer solution containing beta-mercaptoethanol is adopted for decalcifying cartilage, a supercritical CO2 extraction technology is adopted for degreasing, and the collagen is obtained; alkaline protease and collagenase II are adopted for substrate directional enzymolysis and gradient digestion; then the non-denatured type II collagen is separated by adopting three-phase centrifugal separation, and the non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained by adopting bionic H column chromatography purification.

The invention relates to the technical field of ferula asafoetida endophytic fungi separation and purification, in particular to alkaloid compounds in ferula asafoetida endophytic fungi as well as a preparation method and application of the alkaloid compounds in the ferula asafoetida endophytic fungi. And extracting with dichloromethane to obtain dichloromethane part extract, and purifying and separating the dichloromethane part extract by adopting silica gel column chromatography and high performance liquid chromatography to obtain the alkaloid compounds in the endophytic fungi of ferula sinkiangensis. The alkaloid compound in the ferula sinkiangensis endophytic fungi is disclosed for the first time, the compound is subjected to an in-vitro anti-tumor pharmacodynamic experiment, and according to the experiment result, the alkaloid compound has a certain inhibition effect on MCF-7 cells; therefore, the alkaloid compound can be used for preparing the medicine for preventing and treating the breast cancer, and a new medicine is provided for preventing and treating the breast cancer.

The invention discloses an anti-tumor matrine hydrazine thiazole derivative and a preparation method and application thereof.The preparation method comprises the steps that NaH is added into a first reaction container, then a DMF solution is added, thiosemicarbazide is added into the first reaction container, and the solution is stirred; adding sophocarpidine into the stirred solution to perform reflux reaction, performing quenching reaction after the reaction is finished, and performing extraction, concentration drying and column chromatography separation to obtain a thiosemicarbazide sophocarpidine intermediate; adding the obtained thiosemicarbazide matrine intermediate into a reaction container II, and adding a dissolving solvent ethanol for dissolving; adding alpha-bromo-R-ethyl ketone into the dissolved solution for reaction, concentrating and drying after the reaction is finished, and performing column chromatography separation to obtain the anti-tumor matrine hydrazine thiazole derivative. The anti-tumor matrine hydrazine thiazole derivative is high in anti-tumor activity, the preparation method is simple and convenient, and reaction conditions are easy to control.

The present invention provides a cornus officinalis fruit extract rich in morroniside and a preparation method and application thereof, and relates to the technical field of plant natural products. The cornus officinalis fruit extract rich in morroniside comprises morroniside, strychnoside and swertiaside, wherein the content of morroniside is 10% to 12%. The cornus officinalis fruit extract rich in morroniside of the present invention has excellent anti-inflammatory effect, can promote skin firmness and reduce wrinkles, and can be widely used in the preparation of cosmetics and skin care products. The preparation method of the cornus officinalis fruit extract rich in morroniside of the present invention efficiently extracts the active components in the cornus officinalis fruit by ethanol extraction and enzymatic hydrolysis treatment, and obtains the cornus officinalis fruit extract enriched with morroniside by combining column chromatography. The method is simple in process and has strong operability.

The invention provides a separation and purification method of urokinase, and relates to the technical field of separation and extraction. The separation and purification method comprises the following steps: S1, regulating the pH value of male urine to 8.5, standing, and taking supernate; s2, mixing the supernate with an adsorption material, and eluting with an eluent to obtain a urokinase crude product solution; the adsorption material is a core-shell silica gel polymer, Fe3O4atSiO2-NH2 is taken as a core, and polystyrene is taken as a shell; the eluent is a sodium citrate buffer solution with the concentration of 0.5 to 0.15 M, acetonitrile with the concentration of 1 to 5 weight percent and EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 0.5 to 1.5 mM; and S3, carrying out anion exchange column chromatography on the urokinase crude product solution, eluting with an eluent, and precipitating to obtain the urokinase. The urokinase prepared by the method has the advantages of high polymer urokinase content, high recovery rate and high activity yield.

The invention belongs to the field of natural product extraction, and relates to a method for extracting and separating flavonoid compounds from Artemisia chrysantha, which comprises the following steps: carrying out reflux extraction on the dry overground part of Artemisia chrysantha by using 70% ethanol, loading to a balanced D101 macroporous adsorption resin column, sequentially eluting by using water, a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution, and collecting the eluent. Four fractions from Fr. I to Fr. IV are obtained; respectively carrying out polyamide column chromatography separation on Fr.II to Fr.IV, and sequentially eluting with a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution to respectively obtain three fractions; and further purifying the Fr.II-1, the Fr.II-2, the Fr.III-1, the Fr.III-2, the Fr.III-1 and the Fr.III-3 by virtue of a Sephadex LH-20 column, so as to finally obtain seven flavonoid compounds. According to the method, only the ethanol water low-toxicity solvent is used for extraction and preparation, organic solvents with high toxicity are not used, the method is green and environment-friendly, the extraction efficiency is high, the number of extracted compounds is large, chemical components of the artemisia europaea medicinal material are enriched, the resource source of active components is widened, and a foundation is laid for follow-up research.

The invention discloses an efficient separation and purification method of MLnM type medium-chain and long-chain triglyceride, and belongs to the technical field of separation and purification. The efficient separation and purification method comprises the following steps: S1, preparing a crude product; s2, mixing the crude product with an adsorbent for static adsorption; and S3, performing column chromatography elution on the static adsorption product, and performing dynamic chromatography to separate the MLnM type triglyceride. According to the efficient separation and purification method of the MLnM type medium-long-chain triglyceride, a neutral aluminum oxide static adsorption and dynamic column chromatography coupling process is adopted, the purity of the MLnM type triglyceride can be greatly improved and is far higher than that of other adsorbents, the whole process steps are clear, the steps of raw material preparation, static adsorption, dynamic chromatography and the like are included, and the method is suitable for industrial production. The operation process is easy for standardized and large-scale production, and does not need to depend on complex equipment such as molecular distillation, supercritical CO2 extraction and the like.

The invention relates to the technical field of ferula asafetida endophytic fungus strain separation and purification, in particular to an alkaloid component in pleurotus nebrodensis as well as a preparation method and application thereof, and the alkaloid component in pleurotus nebrodensis, namely an alkaloid compound in pleurotus nebrodensis, is prepared by the following steps: crushing pleurotus nebrodensis sporocarp, adding methanol, heating, refluxing and extracting; dispersing the pleurotus nebrodensis total extract into a suspension with water, and extracting to obtain a petroleum ether part and ethyl acetate part extract; and carrying out gradient elution on the ethyl acetate part extract by silica gel column chromatography, and separating and purifying the third fraction by high performance liquid chromatography to obtain the alkaloid compounds in pleurotus nebrodensis. The invention discloses the alkaloid component in the pleurotus nebrodensis, namely the pleurotus nebrodensis alkali C for the first time, and the pleurotus nebrodensis alkali C has a certain inhibition effect on HGC-27 cells, so that the alkaloid component in the pleurotus nebrodensis, namely the pleurotus nebrodensis alkali C, can be applied to the preparation of tumor prevention drugs and antitumor drugs.

The invention discloses a novel ansamycin compound as well as a preparation method and application thereof, and belongs to the technical field of compounds and preparation thereof. The amycolatopsis sp. YINM00005 is endophyte of peperomia dindygulensis in Yunnan, and a large amount of novel ansamycin compounds can be metabolized after fermentation; the fermentation method mainly comprises the following steps: fermenting the strain under proper culture conditions to obtain fermentation liquor, extracting and concentrating by a solvent, and combining with column chromatography to prepare 10 new compounds, the total concentration of the 10 new compounds in the fermentation liquor can reach 3.5-6.8 mg / L, the purity after extraction and separation can reach more than 99%, and the 10 compounds have relatively strong anti-tumor cytotoxic activity. The method is low in cost, simple in process and environment-friendly.

The invention discloses an electro-synthesis method of alpha-aminomethyl tetrazole, which comprises the following steps: by taking a platinum sheet as a cathode, a carbon rod as an anode and tetrabutylammonium tetrafluoroborate as an electrolyte under the catalysis of constant current, coupling tertiary amine, isonitrile and azido trimethylsilane by a one-pot method to prepare an alpha-aminomethyl tetrazole derivative, and carrying out post-treatment on the obtained reaction mixed solution, and carrying out column chromatography separation to obtain a pure alpha-aminomethyl tetrazole product. The alpha-aminomethyl tetrazole can be obtained only through one-step reaction, the operation steps are few, a large number of oxidizing agents and additives are not used, the post-treatment process is simple, and the product is easy to separate.

The invention discloses a method for preparing various bioactive peptides based on bean dreg fermentation, which comprises polypeptides with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and belongs to the field of microbial biological fermentation. Bacillus amyloliquefaciens sourced from fermented soybeans is used for fermentation, and a mixed product with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity is obtained. The invention also provides a method for purifying the polypeptide with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, which is characterized in that the mixed product is purified by combined column chromatography, the combined column chromatography comprises cation exchange resin LXP-01, macroporous adsorption resin HP20, weak base anion exchange resin D941, silica gel resin and Sephadex LH-20. The invention also discloses seven bioactive peptides with biological activity, the amino acid sequences are CKLLL, APGYSC, FPKYG, FPGPLV, FPRSH, FMAV and SVCC, and the bioactive peptides have good alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and can be used for producing functional foods, health care products, cosmetics and the like for assisting in reducing blood sugar, reducing blood pressure, resisting oxidation and the like.

The invention relates to the technical field of extraction and separation of compounds in traditional Chinese medicinal materials, in particular to a method for simultaneously separating and purifying three pentacyclic triterpenoids from a tripterygium hypoglaucum root medicinal material. Three pentacyclic triterpenoid monomers are obtained through the steps of smashing, reflux extraction, normal-phase silica gel chromatography, reversed-phase high-pressure column chromatography, recrystallization and the like, the method is high in extraction efficiency and small in reagent dosage, the three pentacyclic triterpenoid compounds can be obtained at the same time, and the obtained compounds are high in purity and yield, have high social and economic value and are suitable for industrial production. The purity of the obtained plasmin, tripterine and demethylzeylasteral is high, and a foundation is laid for the preparation and production of reference substances of triterpenoid components in the roots of pyracantha fortunei, the subsequent pharmacological activity and the development of new immune drugs.

The invention discloses a preparation method and application of a novel glycoside component in eupatorium chinense. The compound is separated from an eupatorium chinense extract. The eupatorium chinense root extract is separated by using an extraction method, macroporous adsorption resin impurity removal, HW-40F gel column chromatography, high performance liquid chromatography and other methods to obtain a new glycoside compound. The inhibitory activity of the separated compound on diabetes related targets alpha-glucosidase and protein tyrosine phosphatase 1B (PTP1B) is tested, the experimental result shows that the compound has good inhibitory activity on two enzymes, and the molecular docking experiment also shows that the compound has good inhibitory activity on the two enzymes. The compound has good binding energy with two target protein alpha-glucosidase and protein tyrosine phosphatase 1B. Therefore, the compound can be used for preparing medicines for preventing, delaying or treating alpha-glucosidase or PTP1B-mediated diseases, especially type II diabetes and obesity, or can be used as a lead compound of the medicines.

The invention relates to the technical field of indole alkaloid compounds, in particular to indole pleurotus nebrodensis alkali A, a preparation method thereof and application of the indole pleurotus nebrodensis alkali A to preparation of drugs for preventing and resisting tumors. Pleurotus nebrodensis sporocarp is smashed, ethyl alcohol is added, heating reflux preparation and concentration are conducted, obtained pleurotus nebrodensis total extract is dispersed into suspension with water, and then the suspension is placed in a drying oven to be dried to obtain the indole pleurotus nebrodensis alkali A. The indole pleurotus nebrodensis alkali A is obtained. The method comprises the following steps: sequentially extracting with petroleum ether, dichloromethane and ethyl acetate, concentrating an extracting solution to obtain ethyl acetate part extract, carrying out gradient elution separation on the ethyl acetate part extract by silica gel column chromatography, carrying out gradient elution on the obtained third fraction by high performance liquid chromatography, purifying and separating, and collecting eluate to obtain the indole pleurotus nebrodensis alkali A at 16.3 minutes. The indole pleurotus nebrodensis alkali A is disclosed for the first time, and it is found through in-vitro antitumor pharmacodynamic experiments that the indole pleurotus nebrodensis alkali A has a certain inhibition effect on human HGC-27 gastric cancer cells, so that the indole pleurotus nebrodensis alkali A can be used for preparing drugs for preventing and resisting gastric cancer.

The invention discloses a novel benzofuran component in eupatorium chinense and application of the benzofuran component in anti-inflammatory activity. The obtained compound is separated from an eupatorium chinense extract. The eupatorium chinense root extract is separated by using an extraction method, macroporous adsorption resin impurity removal, Sephadex LH-20 gel column chromatography, high performance liquid chromatography and other methods. According to the present invention, all the separated compounds and the analogues thereof have good inhibition activity on target cyclooxygenase-2 of inflammation-related diseases, and have good inhibition activity on COX-2 enzyme, and the molecular docking experiment results show that the compounds and the target proteins have good binding energy. Therefore, the compound can be used for preparing drugs for treating inflammatory related diseases, or anti-inflammatory and analgesic drugs, or tumor drugs by inhibiting COX-2 enzyme, such as drugs for rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, Alzheimer's disease and the like, and related natural lead compounds are provided.

The preparation method comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol respectively to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the isopentenyl flavone compound extracted from epimedium sagittatum, thereby obtaining the isopentenyl flavone compound extracted from epimedium sagittatum. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and two compounds are respectively obtained, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The invention discloses a crystallization method of recombinant human insulin, which belongs to the technical field of biological medicine, and comprises the following steps: taking a sample containing recombinant human insulin, carrying out column chromatography salt conversion to obtain a salt conversion eluent, then adding purified water, adjusting the pH value to prepare a solution to be crystallized, and adding an acid solution, a zinc salt solution and arginine to obtain a crystallization solution; and adjusting the pH value and temperature of the crystallization liquid, and crystallizing to obtain the hexahedral crystal. According to the method, the to-be-crystallized solution is prepared from the purified and prepared qualified eluent containing the human insulin in a chromatography salt conversion mode, and the preparation mode of the to-be-crystallized solution is simple, efficient and easy to operate; the crystallization liquid system is simple, only acid, zinc salt and arginine need to be added into the to-be-crystallized solution, phenols and organic solvents do not need to be added, and chemical substance residues are avoided. The residual salt and the organic solvent in the salt conversion concentrated solution are fully utilized to participate in the crystallization reaction, the solvent does not need to be repeatedly added, the reagent is saved, and the process time is shortened.

The invention relates to an extraction and purification method of green tea cell wall polysaccharide with hypoglycemic activity and the obtained green tea cell wall polysaccharide. The green tea is extracted by using water, CDTA, saturated sodium carbonate, 1M NaOH solution and 4M NaOH solution through a sequential extraction method to obtain five different tea polysaccharides; the green tea cell wall polysaccharide with the best hypoglycemic activity is screened out through activity inhibition of glycometabolism enzyme, and is further purified by gel column chromatography. Compared with the prior art, the method has the advantages that the reaction conditions in the whole operation process are mild, the activity of the green tea cell wall polysaccharide can be reserved to the greatest extent, the loss of polysaccharide components is reduced to the greatest extent, the extraction rate is high, the energy consumption is low, the solvent dosage is small, the operation is simple, and the economic cost is low; a foundation is laid for researching the physicochemical property and biological activity of the green tea cell wall polysaccharide, and the method has important significance on utilization of the green tea cell wall polysaccharide in the fields of food, medical care, cosmetics and the like.

The invention discloses a method for extracting nobiletin from citrus peel, which comprises the following steps: S1, crushing dried citrus peel, adding an alcohol-water solution into the citrus peel powder, heating and extracting to obtain a citrus peel extracting solution; s2, centrifuging the citrus peel extracting solution, collecting supernate, and filtering to obtain filtrate; s3, adding an adsorbent into the filtrate, stirring, standing, filtering, concentrating the filtrate, and recrystallizing to obtain nobiletin with the purity higher than 98%; the adsorbent is selected from at least one of diatomite, attapulgite and porcellanite. According to the method, the alcohol-water solution is added to extract the citrus peel, the adsorbent is added to adsorb the citrus peel extracting solution, high-purity nobiletin can be extracted from the citrus peel, meanwhile, the adsorbent is added into the extracting solution, the extraction rate of the nobiletin can be increased, and the method has the advantages of being easy to operate, low in cost and the like. Expensive production equipment or column chromatography is not needed, and the like.

The invention discloses an extraction method of coriolus versicolor polysaccharide. The method comprises the steps of crude extraction of the coriolus versicolor polysaccharide and purification of the coriolus versicolor polysaccharide. The crude extraction of coriolus versicolor polysaccharide comprises the following steps: water extraction and alcohol precipitation, H2O2 decoloration and freeze-drying; the purification of the coriolus versicolor polysaccharide comprises the following steps: carrying out anion exchange column chromatography on a coriolus versicolor polysaccharide crude product obtained by crude extraction of the coriolus versicolor polysaccharide, and carrying out ultrafiltration separation and purification to obtain the refined coriolus versicolor polysaccharide. The coriolus versicolor polysaccharide with the polysaccharide content of 80.18% and the protein content of 5.9% can be obtained through the preparation method. HPGPC analysis shows that the coriolus versicolor polysaccharide is uniform in molecular weight distribution, and the relative molecular weight Mw is about 17478 Da. The coriolus versicolor polysaccharide plays a role in treating the NAFLD by regulating intestinal flora and influencing bile acid metabolism, a theoretical basis is provided for treating the NAFLD by the coriolus versicolor polysaccharide, and a direction is broadened for NAFLD medicine development.

The invention belongs to the technical field of heterocyclic compounds, and particularly relates to a synthesis method of an sGC agonist. Adding acid and iron powder into a solvent, stirring and activating, and then adding a compound I to react to obtain a reaction solution; filtering the reaction liquid, and concentrating under reduced pressure to obtain a concentrate; carrying out reflux reaction on the concentrate and malononitrile in a solvent, and cooling to room temperature to obtain a reaction solution; adding the reaction liquid into water, stirring at room temperature to separate out a solid, filtering, adding the obtained filter cake into 1, 4-dioxane, carrying out first reflux pulping, cooling to room temperature, crystallizing, filtering, adding the obtained filter cake into a mixed solution of methanol and ethanol, carrying out second reflux pulping, cooling to room temperature, filtering, decoloring and filtering the obtained filter cake, adding the filtrate into water, stirring at room temperature, crystallizing, filtering, and drying to obtain a solid. And carrying out vacuum drying on the obtained filter cake to obtain the sGC agonist. The raw materials are easy to obtain, operation such as use of a microwave reactor and column chromatography is avoided, industrial amplification is facilitated, and the environmental protection cost is effectively reduced.