155 results about "Column chromatography" patented technology

The application provides a method for synthesizing an eplerenone intermediate N-1 by one-pot method, and belongs to the technical field of organic synthesis. The method is characterized in that: the eplerenone intermediate N-4 is dissolved in dichloromethane, a potassium carbonate aqueous solution and dibromohydantoin are added to perform an opening ring reaction; after the reaction is completed, hydrochloric acid is directly added to perform a rearrangement reaction; then water is separated and methanol is added in the organic layer to perform an ozonation reaction; then sodium sulfite is added to perform a reduction reaction; then hydrochloric acid is added to perform a methyl esterification reaction, so as to obtain the eplerenone intermediate N-1 crude product, and finally the eplerenone intermediate N-1 fine product is obtained through refining. The method realizes the one-pot synthesis of the eplerenone intermediate N-1 from the eplerenone intermediate N-4 through the continuous reactions of opening ring, rearrangement, ozonation, reduction and methyl esterification, and can avoid column chromatography purification, so that the method becomes a green chemical process route with greatly reduced organic solvent consumption and wastewater.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

The application discloses a Dendrobium nobile Lindl polysaccharide component group, a preparation method thereof and application of the Dendrobium nobile Lindl polysaccharide component group in immunoregulation. Four polysaccharide components with definite structures, namely DNP-0-1 (>100 kDa), DNP-0-2 (30-100 kDa), DNP-0-3 (<30 kDa) and DNP-1-1 (<50 kDa), are synchronously separated from Dendrobium nobile Lindl by combining ultrasonic extraction process optimized by a response surface method with DEAE-cellulose column chromatography and ultrafiltration membrane series grading technology. After structure characterization and in-vitro immunological activity evaluation, the four components all have immunoregulatory activity, and the activity of DNP-0-1 is optimal. Pearson correlation analysis shows that the content of galactose and the molecular weight are extremely significantly positively correlated with the immunological activity, and the content of glucose and mannose is significantly negatively correlated with the activity. The application establishes an integrated research system of 'preparation by grading-structure characterization-activity screening-structure-activity analysis', and provides a material basis and evaluation basis for the development of immunoregulatory drugs or functional foods.

The application belongs to the technical field of microbial fermentation, and particularly relates to a kind of cytolysis product of mixed bacteria fermentation and a preparation method. The cytolysis product is prepared by mixed bacteria fermentation of two kinds of aerobic probiotics, i.e. Lactobacillus plantarum and Aspergillus oryzae are used as fermentation strains to carry out mixed bacteria fermentation treatment on rice milk, and membrane filtration and column chromatography system are used for filtration and enrichment, so as to obtain a cytolysis fermentation product filtrate rich in kojic acid, the content of kojic acid is greater than 200 g / L, which is significantly improved compared with conventional process. The obtained cytolysis product has no bad smell and clear color, so that the beneficial function of kojic acid can be played only with a low addition amount in cosmetics, and the addition of odor masking and color correcting auxiliaries is reduced, and the cytolysis product can be used for preparing cosmetics with skin repair, whitening and other functions.

The present application provides a method for extracting complex organic acid from the waste liquid of the extract of Rubus corchorifolius L.f. The waste liquid of the column chromatography effluent of the Rubus corchorifolius L.f. extract is taken, the specific gravity of the secondary waste liquid is adjusted to obtain the column loading liquid, the column loading liquid is passed through a resin column A, water washing is performed, the column effluent and the water washing liquid are collected to obtain a combined liquid 1, the combined liquid 1 is passed through a resin column B, water washing is performed, reagent C is used for elution, and the eluate is collected. The combined liquid 2 is obtained by passing through a resin column D and collecting the effluent and the water washing liquid, vacuum concentration is performed, crystallization is performed, and filtration is performed to obtain quinic acid.

This invention discloses a benzofuran compound and its application in lowering uric acid activity. Compounds 1-3 were isolated from the fermentation products of the endophytic fungus *Septoriella phragmitis*, with compound 1 being a novel compound. The fermentation products were purified using mixed solvent extraction, normal silica gel column chromatography, and high-performance liquid chromatography to obtain compounds 1-3. The inhibitory activity of compounds 1-3 on xanthine oxidase, a target related to gout symptoms, was tested. Experimental results showed that the novel compound 1 exhibited good inhibitory activity against xanthine oxidase. Molecular docking experiments also demonstrated that compound 1 had a good binding energy to the target protein. Therefore, compound 1 can be used to prepare a natural lead compound for delaying or treating diseases caused by xanthine oxidase, providing a potential therapeutic agent for hyperuricemia and gout.

The application relates to the technical field of natural medicinal chemistry, and specifically discloses a triterpenoid compound and application, and a preparation method of the compound. The preparation method comprises the following steps: drying and crushing schisandra chinensis roots to obtain dried and crushed schisandra chinensis roots, extracting the dried and crushed schisandra chinensis roots by using 70% ethanol water solution under negative pressure cavitation, combining and concentrating the extract to obtain a crude extract, separating the crude extract by using a D101 macroporous adsorption resin, eluting the crude extract by using water, 30% ethanol, 70% ethanol and 90% ethanol in sequence, collecting a 70% ethanol elution part, performing MCI column chromatography on the 70% ethanol elution part, performing gradient elution on the 70% ethanol elution part by using methanol-water, collecting a depigmentation sample, performing ODS column chromatography on the depigmentation sample, performing gradient elution on the depigmentation sample by using water-methanol, collecting a target component, and purifying the target component by using a preparative high performance liquid chromatograph to obtain the target compound. The application separates a novel triterpenoid compound from schisandra chinensis roots for the first time, expands the development range of medicinal resources of schisandra chinensis plants, simultaneously provides a novel structure mother nucleus and a candidate active molecule for anticancer drug research and development, and enriches the research and development reserves of antitumor natural medicines.

This invention belongs to the field of solid waste resource utilization technology. To address the problems of inconsistent temperature during existing fluidized bed waste plastic gasification processes, which prevent graded conversion and result in products primarily consisting of hydrogen and carbon monoxide with low added value and low energy efficiency, this invention provides a device and method for graded gasification of waste plastics to prepare chemicals. The fluidized bed reactor has a top section connected to a silo. The middle section of the reactor is connected to air / oxygen tanks, carbon dioxide tanks, hydrogen tanks, and carbon monoxide tanks. A gas-liquid separator and column chromatography are connected at the top of the reactor, and a liquid pump connects it to a preheater at the bottom. An outlet is located at the bottom. Graded conversion of waste plastics is achieved within the fluidized bed, increasing the added value of the waste plastics and facilitating their graded conversion and high-value utilization. The invention features high production efficiency, continuous production capability, and applicability to other solid waste resource utilization processes.

The application provides a preparation method of a benzaldehyde aniline compound and relates to the technical field of organic synthesis. The preparation method provided by the application comprises the following steps: in a solvent, with an alkali as a promoter, under an atmosphere with an oxygen concentration of 20v100%, the temperature of a reaction system is controlled to be 50-70 DEG C, a diphenyl ketone compound I is reacted with an aniline compound II to generate a benzaldehyde aniline compound III, and the reaction product is subjected to column chromatography separation to obtain a pure benzaldehyde aniline compound III. The method has the beneficial effects that the reaction is simple, green and environment-friendly, the use of an expensive transition metal catalyst is avoided, the reaction condition is mild and controllable, and an environment-friendly byproduct is generated.

This invention discloses a strain of *Carya volvacea* ZZNS-Y2 and its applications, belonging to the field of microbial application technology. The *Carya volvacea* (… Daldinia sp. The preservation number of ZZNS-Y2 is CCTCC NO: M 20241561. The method includes the following steps: *Cyclocarya paliurus* ZZNS-Y2 is cultured on one of the following solid-state fermentation media: corn, potato, corn grits, or rice. After extraction and concentration, the compounds crocusatin C and jasminodiol are separated by silica gel column chromatography, gel column chromatography, and high-performance liquid chromatography. The method of this invention is efficient, pollution-free, has mild reaction conditions, is not limited by plant resources, and is easy to scale up for production.

The application discloses Ixeris sonchifolia Hance polysaccharides, a preparation method and application thereof, belongs to the technical field of plant polysaccharides, and provides polysaccharides ISP-1a and ISP-2a, wherein the ISP-1a has a molecular weight of 2086 Da and is composed of seven monosaccharides of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose; the ISP-2a has a molecular weight of 1781 Da and is composed of five monosaccharides of mannose, rhamnose, galacturonic acid, xylose and arabinose. The whole grass of Ixeris sonchifolia Hance is subjected to hot water extraction after ethanol degreasing, neutral enzyme + Sevage method is used to remove protein, macroporous resin is used for decolorization, column chromatography elution is carried out, purification is carried out, and two kinds of uniform polysaccharides are obtained after freeze-drying. The polysaccharides ISP-1a and ISP-2a have good antioxidant and lipid-lowering effects, and can be applied to the preparation of food, health products or medicines with antioxidant and lipid-lowering functions.

This invention discloses a method for preparing vicinal diamine derivatives, belonging to the field of pharmaceutical intermediates and organic synthesis technology. Specifically, under an inert atmosphere, an aldehyde imine, an N-aryl tertiary amine, a base, a cadmium selenide quantum dot catalyst, and a solvent are mixed and reacted under blue light irradiation for 8-24 hours. After extraction with ethyl acetate and saturated brine, the organic phase is collected, dried, the solvent is removed by vacuum distillation, and purified by column chromatography to obtain the vicinal diamine derivatives. The vicinal diamine derivatives synthesized by this invention can be used as synthetic intermediates and metal ion chelating agents in drug synthesis and fine chemical industries.

The application discloses a pterosin sesquiterpenoid compound and a preparation method and application thereof, belongs to the technical field of biological medicines, aims at the problems of a research blank of pterosin sesquiterpenoid compounds in Anemone raddeana and a lack of structure-confirmed anti-inflammatory active monomers, and provides a pterosin sesquiterpenoid compound with a structural formula as shown in the following.The compound preparation needs to first crush dried whole grass of Anemone raddeana, extract by heating and refluxing with an organic solution, concentrate under reduced pressure to obtain total extract; then, the extract is extracted by petroleum ether, ethyl acetate and n-butanol step by step, the ethyl acetate part extract is enriched, and the high-purity product is prepared through multi-step purification of a silica gel column, a C-18 reverse phase column, a gel column and semi-preparative liquid chromatography.In addition, the compound can dose-dependently inhibit the NO release of LPS-induced macrophages, and can be used for preparing anti-inflammatory drugs for treating or preventing inflammatory diseases related to excessive production of nitric oxide, fills the research blank, and provides a high-quality candidate component for anti-inflammatory drug research and development.

The application discloses radix sophorae tonkinensis polysaccharide STGP-1, a preparation method thereof and pharmaceutical use for treating lung squamous carcinoma, and relates to the technical field of medicines. The monosaccharide composition of STGP-1 includes fucose Fuc, arabinose Ara, galactose Gal, glucose Glc, xylose Xyl and mannose Man, and the molar ratio is 0.34:12.15:25.76:57.07:1.06:3.62. The STGP-1 is obtained by extraction, separation and purification from dried roots and rhizomes of radix sophorae tonkinensis, and the preparation method comprises the steps of pretreatment and crude extraction, impurity removal treatment, anion exchange column chromatography separation, gel column chromatography purification and the like. The STGP-1 can be used alone or in combination with anti-PD-1 monoclonal antibody for preparing a medicine for treating lung squamous carcinoma. The preparation process is stable, the source is natural, and the STGP-1 can provide a new high-efficiency and low-toxicity candidate medicine for immune combined treatment of lung squamous carcinoma.

The present invention provides a method of isolating arjunetin from the bark of Terminalia arjuna. The invention provides a simplified, cost-effective method and scalable method for isolation of arjunetin from the Terminalia bark. The present invention encompasses an ethanolic extract of arjunetin that comprises arjunetin with 95% purity. The ethanolic extract of arjunetin is obtained from solvent extraction without using column chromatography. Arjunetin has various therapeutic applications, anti-diabetic, anti-inflammatory, anti-cancer, and anti-viral drugs.

This invention discloses a USP1 inhibitor intermediate and its preparation method, comprising the following steps: compound I reacts with compound II or II' to obtain compound III or III'; compound III or III' is catalytically reduced to obtain compound IV; compound IV is reacted under acidic conditions to generate compound V; compound V undergoes condensation and cyclization to obtain compound VI; compound VI reacts with compound XIV under alkaline conditions to obtain compound VII; compound VII is hydrolyzed to obtain compound VIII; compound VIII is condensed with trifluoroacetic anhydride to obtain compound IX; compound IX reacts with an amination agent to obtain compound X; compound X is dehydrated to generate compound XI; compound XI undergoes a reduction reaction to obtain compound XII; and compound XII undergoes a halogenation reaction to obtain the USP1 inhibitor intermediate (compound XIII). This route is mild, simple to operate, and has a high overall yield. Furthermore, the process is stable with few byproducts, and post-processing does not require column chromatography, making it suitable for industrial production.

The application discloses a kind of butyrolactone compound in preparation treatment renal clear cell carcinoma drug purposes, characteristic is the compound structural formula as shown in I, the purposes of the compound in preparation anti renal clear cell carcinoma tumor drug and in preparation promote renal clear cell carcinoma sorafenib treatment sensitivity drug, its preparation method is by carrying out fermentation culture to marine fungus, obtains fermentation, then the fermentation is soaked with ethyl acetate and is extracted to obtain crude extract, again the crude extract is sequentially separated by normal phase medium pressure column chromatography, reversed phase medium pressure column chromatography and semi-preparative reversed phase high performance liquid chromatography separation and purification is obtained, advantage is that renal clear cell carcinoma can be significantly enhanced to sorafenib treatment sensitivity, can effectively inhibit tumor growth and delay the emergence of drug resistance.

The present invention provides novel water-soluble compositions comprising sesquiterpene coumarin enriched fractions containing different ratios of sesquiterpene coumarin derived from Ferula species for the treatment of Parkinson's disease via inhibition of MAOenzyme (MAO-A and MAO-B). The invention also provides a method of preparation of ethyl-acetate extract and sesquiterpene coumarin enriched fractions from asafoetida (Hing) that is oleo-gum-resin of Ferula assafoetida using silica gel column chromatography wherein the mobile phase consists of differing compositions of hexane, toluene, chloroform,ethyl-acetate, and methanol. The invention provides the method to prepare the formulation using a multistep process comprising of preparing sesquiterpene coumarin enriched fraction followed by preparing water-soluble formulation. The final composition improves the water solubility, imparts long-term stability, exerts MAO inhibitory activity, and improves pharmaceutical compliance.

This invention discloses a rapid and accurate method for simultaneously determining the content of multiple new pollutants in water, belonging to the field of new pollutant detection technology. The method involves adding a compound salt and water sample to a narrow-necked, pear-shaped, flat-bottomed flask and shaking well. An internal standard solution is then added to the flask, and after shaking again, an organic extraction solvent is added. The flask is then subjected to high-frequency extraction in a vortex mixing extraction device, followed by standing. The upper organic phase in the flask is transferred, centrifuged, and the supernatant is used for ESI-HPLC-MS / MS-MRM analysis. The advantages of this invention are: by synergistically enhancing extraction through compound salting-out and mixed solvents, combined with techniques such as phenyl column chromatography and rapid gradient elution, it solves the problem of simultaneous extraction, separation, and quantification of new pollutants. Pretreatment eliminates the need for concentration, dissolution, and dehydration steps, achieving non-co-current separation of three types of substances within 10 minutes, significantly shortening the analysis time. This method is suitable for the simultaneous rapid screening and quantitative analysis of new pollutants in water bodies.

The present application relates to a method for purifying rubitecan and its intermediates. The method is simple, low cost, high yield, suitable for industrial production, and avoids the uncertainty of silica gel column chromatography purification.

This invention provides a method for preparing tert-butyl 2-hydroxymethylbenzoate, the method comprising the following steps: (1) reacting tert-butyl hydrogen phthalate and isobutyl chloroformate to obtain compound 1, (2) reacting compound 1 to obtain tert-butyl 2-hydroxymethylbenzoate; the preparation method provided by this invention has a high yield and stable process, the post-processing is relatively simple, it can avoid operations such as column chromatography, and the reagents used are all conventional reagents, which reduces the production cost and is applicable to industrial scale-up production, providing technical support for the reduction of carboxylic acid compounds into primary alcohols.

This invention discloses a preparation process for a homogeneous immunomodulatory polysaccharide from *Gynostemma pentaphyllum* and its application in the preparation of anti-melanoma drugs and immunomodulators, relating to the field of pharmaceutical manufacturing technology. The preparation process includes four steps: compound enzyme-ultrasound synergistic extraction, fractional alcohol precipitation to remove proteins, DEAE-52 ion exchange column chromatography, and Sephacryl S-400 gel permeation chromatography purification. The resulting homogeneous polysaccharide (GOP-3) has a weight-average molecular weight of 12±1 kDa and is composed of mannose, glucose, and galactose in a specific ratio. The GOP-3 of this invention has a weight-average molecular weight (Mw) of 12.8 kDa, a dispersion (Mw / Mn) < 1.2, a uronic acid content of 23.5%, a well-defined structure, and is suitable for drug application. Furthermore, GOP-3 can significantly promote the secretion of IL-12 by dendritic cells and induce the differentiation of naive T cells into Th1 cells. Simultaneously, compared with a full-dose PD-1 monoclonal antibody, the combination of GOP-3 and a half-dose monoclonal antibody not only reduces the risk of immune-mediated pneumonia caused by antibody drugs but also increases the tumor inhibition rate by more than 40%.

This invention discloses a method for preparing and purifying 5-hydroxymethylfurfural crystals. The method involves reacting a sugar source and an acid catalyst in a first solvent to obtain a reaction solution containing crude 5-hydroxymethylfurfural. The solution is then purified according to the following steps: the first solvent is removed by rotary evaporation, and a second solvent is added in the presence of water for extraction. The resulting organic phase is then subjected to rotary evaporation to remove the second solvent. Water and activated carbon are added to the concentrate for decolorization, followed by filtration. The first solvent is a water-soluble solvent, and the second solvent is a water-insoluble nonpolar solvent. The resulting filtrate is then subjected to rotary evaporation and freeze-drying to remove moisture. The dried crystals are then separated by column chromatography to obtain high-purity 5-hydroxymethylfurfural crystals. This method features a simple reaction system, mild reaction conditions, and lower processing costs, providing a new solution for the preparation and purification of 5-hydroxymethylfurfural crystals.

This invention relates to the fields of biomedicine and microbial fermentation engineering technology, and discloses a strain of *Streptomyces citrus* YS-4 that produces mesocyclocarpine. The separation and purification process includes: solid-liquid separation of the fermentation broth; extraction of the supernatant with ethyl acetate and extraction of the bacterial cells by methanol soaking; combining and concentrating the extracts to obtain a crude extract; purification of the crude extract by silica gel column chromatography and Sephadex LH-20 gel column chromatography; and finally purification by C18 reversed-phase semi-preparative high-performance liquid chromatography to obtain mesocyclocarpine. This invention employs a dual extraction strategy to improve the yield and achieves efficient separation of the target product and its structural homologues through specific chromatographic conditions, effectively solving the problem of incomplete extraction caused by the coexistence of intracellular adsorption and extracellular secretion of the target product, resulting in a final product yield superior to that of single-solvent extraction methods.

A functionalized covalent organic framework material containing dimethylamino and its preparation method are disclosed. This invention relates to a C2-type aldehyde monomer containing a dimethylamino functional group and a functionalized covalent organic framework material containing dimethylamino and its preparation method. The purpose of this invention is to solve the problems of insufficient functionalized monomers, easy pore blockage leading to decreased specific surface area, and insufficient selectivity and pH responsiveness in existing COF materials used for the adsorption and separation of biomolecules such as peptides. The method includes: 1. Weighing; 2. Anhydrous and anaerobic reaction; 3. Filtration; 4. Column chromatography purification; 5. Drying; 6. Weighing; 7. Anhydrous and anaerobic reaction; 8. Washing; 9. Deep washing with a Soxhlet extractor; 10. Drying and grinding. The invention innovatively introduces a new monomer with a basic recognition site, and through a "bottom-up" strategy, directly and uniformly introduces the dimethylamino functional group into the COF framework. The material exhibits good crystallinity and a high specific surface area (up to 2481 m²). 2 ( / g), and has significant pH responsiveness.

The application relates to the technical field of plant chemistry and active ingredient heterocyclic compounds of traditional Chinese medicine, and discloses a C17 sesquiterpene alkaloid, a preparation method and application, wherein the C17 sesquiterpene alkaloid and a pharmaceutically acceptable salt thereof are shown as formula I; the C17 sesquiterpene alkaloid of formula I is separated from an ethanol extract n-butanol extraction phase of Dendrobium huoshanense stems through three-step operations of silica gel column chromatography, acid solution and base precipitation and recrystallization; the structure of the compound is determined through nuclear magnetic resonance, high-resolution mass spectrometry and single crystal diffraction analysis; experiments show that the C17 sesquiterpene alkaloid can effectively inhibit the survival and proliferation of A549 lung cancer cells, has good anti-lung cancer activity, and can be used for preparing anti-lung cancer drugs.

This invention discloses a novel C20-type diterpenoid alkaloid compound, its preparation method, and its applications. The molecular formula of the C20-type diterpenoid alkaloid compound is C... 29 H 36 N2O2. This invention, through systematic and in-depth research on the chemical composition of Delphinium spp., uses the whole herb of Delphinium spp. as raw material. Through alcohol extraction, silica gel column chromatography, and preparative liquid chromatography purification, multi-spectral data analysis shows that a new C20 diterpenoid alkaloid compound was isolated from Delphinium spp. This compound is a novel compound reported for the first time. Antitumor activity studies have shown that this C20 diterpenoid alkaloid compound has good inhibitory activity against colon adenocarcinoma cells and can be used to develop plant-derived antitumor drugs.

This invention discloses a compound of formula I and a method for preparing minocycline hydrochloride. The method for preparing compound I involves dissolving 6-demethyltetracycline in acidic water, adding a chlorination reagent for chlorination, then adding p-aminobenzenesulfonic acid diazonium hydrochloride and alkaline solution for coupling reaction, followed by post-treatment. The method for preparing minocycline hydrochloride uses 6-demethyltetracycline as a starting material, proceeding through the intermediate of compound I, and removing the C7 chlorine, p-aminobenzenesulfonic acid group, and C6 hydroxyl group via catalytic hydrogenation, followed by reductive amination and post-treatment. This invention achieves low-pressure palladium-carbon catalytic removal of the C6 hydroxyl group by introducing an electron-donating amino group at the C7 position, eliminating the need for expensive rhodium-carbon catalysts. The reaction conditions are mild, column chromatography purification is unnecessary, the overall yield is ≥85%, and the product purity is ≥96%, making it suitable for industrial production.

This invention provides a chlorine atom transfer addition product and its preparation method, wherein the general structural formula of the chlorine atom transfer addition product is as follows. The preparation method includes: dissolving a copper salt, a phosphine ligand, and a nitrogen ligand in a solvent, stirring under a nitrogen atmosphere to obtain a mixture; subsequently adding an inert chlorinated derivative and an alkene to the mixture and reacting under light; after the reaction is complete, drying and vacuum concentrating to obtain a crude product; and separating the crude product by column chromatography to obtain the chlorine atom transfer addition product; wherein the molar ratio of the alkene to the copper salt, phosphine ligand, and nitrogen ligand is 1:(0.01-0.15):(0.01-0.1):(0.01-0.1). This method achieves a balance between structural diversity and synthetic efficiency through the synergistic catalysis of the copper salt, phosphine ligand, and nitrogen ligand. The structure of the obtained chlorine atom transfer addition product provides a rich molecular framework for drug design, making it promising for applications in medicine, materials, and other fields.

Present invention relates to a method for producing green fluorescent paint for use as a lighting for energy-efficient highway lane and park markings, characterized as comprising following production steps; adding 9,10-dibromoanthracene, CuI, Pd catalyst, diisopropylamine and toluene in a reaction flask under nitrogen atmosphere and stirring them on a magnetic stirrer, adding tetraphenylsilanacetylenyl to the mixture and performing reflux distillation by increasing the reaction temperature, after 24 hours bringing the temperature to room temperature, obtaining pure product from the mixture brought to room temperature using column chromatography, taking 0-1% by mass of the product and mixing it in resin and an aromatic solvent for 6-24 hours.