105 results about "Nobiletin" patented technology

The invention relates to a natural pharmaceutical composition, a preparation method thereof and an application of the natural pharmaceutical composition in preparation of attenuated synergistic medicaments in cancer radiotherapy and chemotherapy. The natural pharmaceutical composition is prepared from extract obtained by decocting and concentrating ginger and dried orange peel decoction, gingerol, dried orange peel water extract and nobiletin according to the ratios of (3-5):(2-9):(1-6):(2-5). The composition has an excellent effect on treating emesis and other side effects caused by cancer radiotherapy and chemotherapy, and can simultaneously play a role in inhibiting tumors.

The invention discloses a method for extracting high-purity nobiletin from citrus peels. The method comprises the following steps: 1, carrying out reflux extraction of crushed dry citrus peels for 2 to 4 times, combining the filtrate after filtration, and concentrating under reduced pressure to obtain a concentrated solution; 2, adding water into the concentrated solution, then adding an acidic solution or an alkaline solution till the pH value of the system ranges from 3 to 9, standing for removal of the supernatant to obtain a sediment, settling and washing the sediment, centrifuging, and collecting solid to acquire a polymethoxylated flavonoids crude product; 3, conducting ungrease treatment; 4, using an organic solvent to achieve reflux dissolution of the polymethoxylated flavonoids crude product which is subject to ungrease treatment, then adding activated charcoal for reflux decoloration, filtering, standing the concentrated filtrate for crystallization, and filtering the crystallized filtrate to obtain a nobiletin crude product; and 5, recrystallizing to obtain the high-purity nobiletin of which the quality purity is not less than 98 percent. The method provided by the invention has the characteristics of simplicity, feasibility, short production period, no need of expensive production equipment, low cost and the like, and is easy for realizing industrial production of high-purity nobiletin.

The invention discloses a method for simultaneously determining twenty-two flavones and phenolic acids in citrus fruits by adopting high performance liquid chromatography-diode array detector-fluorescence detector (HPLC-DAD-FLD). The method is capable of simultaneously determining twenty-two phenolic compounds in citrus fruits such as gallic acid, synephrine, chlorogenic acid, protocatechuic acid,caffeic acid, p-coumaric acid, rhamnosylvitexin, eriocitrin, ferulic acid, rutin, benzoic acid, narirutin, naringin, hesperidin, diosmin, neohesperidin, quercetin, naringenin, kaempferol, nobiletin,hesperetin, acacetin and the like, derivatization is not needed, and the method is high in accuracy, high in sensitivity and excellent in repeatability.

The invention discloses a stable nobiletin liquid preparation and a preparing method thereof; the stable nobiletin liquid preparation is composed of the following components in parts by volume: 1-10 parts of a nobiletin ethanol solution with a concentration of 1-10 mg/ml; 0.5-3 parts of a polyphenol aqueous solution with a concentration of 10-50 mg/ml; and 50-150 parts of a buffer solution with the pH of 7-8. Polyphenol substances are subjected to oxidative polymerization reaction under a weak alkaline condition, and a layer of uniform hydrophilic thin film is formed on the surface of a hydrophobic drug nobiletin, plays a role in embedding the hydrophobic drug and also is a thermodynamic stable system, so that the stability of nobiletin in an aqueous solution is effectively improved, the drug is prevented from being separated out and precipitates are prevented from being generated.

The invention relates to the field of prevention and/or treatment of calcific aortic valvular disease. The inventors research and find that nobiletin can effectively down-regulate expression of osteogenic differentiation marker genes BMP2 and RUNX2 in valvular interstitial cells, and inhibit calcification of the valvular interstitial cells, thereby inhibiting or delaying valvular calcification, and further provide use of the nobiletin in preparing medicines for prevention and/or treatment of individual calcific aortic valvular disease. The invention provides a new option for non-surgical treatment of the calcific aortic valvular disease.

The invention relates to polymethoxyflavone for treating melanoma. The effective ingredients of the polymethoxylflavone comprise one or multiple of hesperetin, nobiletin, sinensetin, 5-hydroxy-3,6,7,3',4'-pentamethoxylflavone, 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone, and 5-hydroxy-6,7,4'-trimethoxylflavone. The polymethoxylflavone has the advantages that after the polymethoxylflavone acts for 48h, the survival rates of melanoma cell lines A2058 and A375 are respectively 42% and 26%; compared with a contrast group, the inhibition action of the polymethoxylflavone on the tumor growth in a C57b1/6 rat inoculated with the melanoma cell lines A375 is increased by 2.75 times.

The present invention provides a peroxisome proliferator-activated receptor (PPAR) activator, which is free from the problem of side effects, can be taken over a long term and has no characteristics taste. Nobiletin is employed as a PPAR activator. Nobiletin has an excellent PPAR activity and has an excellent adiponectin secretion-promoting effect, and is contained in a large amount in citrus fruits, in particular, in Shiikuwasha (Citrus depressa HAYATA) indigenous to Okinawa (Japan). Since citrus fruits have been consumed for many years, the safety of nobiletin has been proven and besides, nobiletin has a low calorie content. Therefore, it can be taken over a long term. Moreover, because of being tasteless and odorless, nobiletin would not damage the unique taste of a food when added thereto. Therefore, it can be added to foods and taken.

The invention discloses a snap bean preservative and belongs to the technical field of preservatives. The snap bean preservative is characterized by consisting of nobiletin, chitosan, sodium alginate, ascorbic acid, phytic acid, calcium chloride and water. The snap bean preservative is prepared from various selected ingredients, the ingredients give full play to a synergistic effect on the premise of exerting respective functions, and the formula is reasonable; during a use process, the snap bean preservative can be used after the above ingredients are mixed uniformly, is a spray-type preservative and is simple to operate; meanwhile, compared with the prior art, the snap bean preservative is good in film-forming properties, is capable of effectively reducing water evaporation, reducing bacterial pollution, reducing rusty spots, reducing the malondialdehyde content, keeping the rigidity and chlorophyll content of snap beans and prolonging the preservation time, and can be stored at the temperature of 9+/-1 DEG C for 30 days.

The present invention provides a multiple index component and fingerprint determination method of a liver-tonifying eyesight-improving oral liquid, and the method aims at chlorogenic acid, luteoloside, ferulic acid, nobiletin, paeoniflorin and hesperidin. The determination method is simple, strongly-specific, good in stability, high in precision, good in reproducibility and high in sample recovery rate. Further, the present invention also provides a preparation method of the liver-tonifying eyesight-improving oral liquid and the liver-tonifying eyesight-improving oral liquid prepared by the preparation method, and the preparation method comprises the step of the determination method.

The invention discloses a nobiletin derivative or a pharmaceutically acceptable salt thereof as well as a preparation method and application thereof. The nobiletin derivative has a structural formula(I) shown in the description, in the formula, R1, R2, R3 and R4 are respectively selected from hydrogen, halogen, hydroxyl, amino, C1-6 substituted or non-substituted alkoxy, a C1-6 substituted or non-substituted ester group, C1-6 substituted or non-substituted alkamino and a C1-6 substituted or non-substituted amide group; R5 is selected from a C3-9 substituted or non-substituted aromatic ring and a C3-9 substituted or non-substituted aromatic heterocyclic ring; and X is selected from O or NR6. The nobiletin derivative or the pharmaceutically acceptable salt thereof, which is disclosed by theinvention, is novel in structure, and in addition, the compound has an excellent inhibition function on P-gp, can be prepared into a P-gp inhibitor, is capable of treating and/or preventing related diseases caused by P-gp, particularly diseases related to tumor drug resistance, or can be mixed and used with other medicines and used as a drug resistance reversal agent, has a high reversion multiple, and is capable of remarkably improving medicine effects of medicines.

The invention discloses application of nobiletin in preparing a feed additive for improving pork quality. The inventor of the invention finds that the nobiletin can obviously improve the crude protein content of pork, the marbling score, the inosinic acid content and the ratio of margaric acid to arachidic acid to linoleic acid to cis-11-eicosenoic acid and eicosadienoic acid unsaturated fatty acid to total fatty acids, namely can effectively improve the fork quality, without affecting growth performances of pigs, so that the nobiletin is suitable for preparing the feed additive for improving the pork quality.

The invention discloses a nobiletin antigen as well as a preparation method and application thereof. The preparation method comprises the steps of reacting nobiletin and a compound B containing amidogen and carboxyl at the same time, so as to obtain a compound C; carrying out coupled reaction on the compound C and N-hydroxy succinimide under the existence of dicyclohexyl carbodiimide or 1-ethyl-(3-dimethylamino propyl)carbonyl diimine hydrochloride to obtain a compound D; carrying out coupled reaction on the compound D and carrier protein to obtain the nobiletin antigen. The nobiletin antigenis applied to preparing an enzyme linked immuno sorbent assay kit for detecting nobiletin in a sample, a luminescence immune kit of nobiletin, or an immunoaffinity chromatographic column. The preparation method provided by the invention has the advantages of convenience and quickness in obtaining the nobiletin antigen, concise synthesis steps, low synthesis cost and good effect. An antibody obtained through the immunization of the nobiletin antigen is good in specificity and low in minimum detection limit value.

The invention discloses application of nobiletin, and particularly discloses application of nobiletin in preparation of health products or medicines for preventing and/or treating oral cancer. Through the anti-proliferation effects of monomeric compounds, namely hesperetin, naringenin and nobiletin, in rutaceae citrus fruits and peels on human oral epidermoid carcinoma cells, the experiments show that the hesperetin, naringenin and nobiletin have an obvious effect on inhibiting proliferation of human oral epidermoid carcinoma cells, and the effect of the nobiletin on inhibiting oral cancer cells is most obvious, so that the nobiletin can be used for preparing health products and medicines with effects on preventing and treating oral cancer and is suitable for large-scale popularization and application.

The invention belongs to the field of medicines, and particularly relates to a composition for preventing or treating osteoporosis, a preparation of the composition and application of the composition in preventing or treating osteoporosis. Specifically, the composition disclosed by the invention is mainly composed of a GPX4 agonist and a lipoxygenase 15 (Alox-15) inhibitor, and vitamin E is supplemented to play a synergistic effect. In one embodiment, the GPX4 agonist is prepared from one or more of 6-gingerol, 8-gingerol, 10-gingerol and PKUMDL-LC-101-D04, and the GPX4 agonist is prepared from one or more of 6-gingerol, 8-gingerol, 10-gingerol and PKUMDL-LC-101- And the Alox-15 inhibitor is prepared from one or more of quercetin, ferulic acid, baicalein, hesperetin, nobiletin, PD146176, orange flavone and ML351. In another embodiment, the composition comprises a ginger extract, a sea buckthorn extract, and a wheat germ extract. Pharmacological experiment results show that the composition can effectively prevent, improve and treat osteoporosis and clinical related symptoms.

The invention provides a compound drug capable of resisting the prostatic cancer. The compound drug consists of a polymethoxyflavone drug and an androgen receptor inhibitor drug, wherein the mass ratio of the polymethoxyflavone drug to the androgen receptor inhibitor drug is 1:1, the polymethoxyflavone drug is nobiletin, and the androgen receptor inhibitor drug is bicalutamide. The polymethoxyflavone drug and the androgen receptor inhibitor anti-cancer drug are jointly used, a drug function is obviously superior to single drug utilization, and therefore, the compound drug has a good inhibitioneffect on inhibiting the prostatic cancer and preventing the prostatic cancer to develop to malignance. The drug provided by the invention is low in a use dosage, and lowers the concentration of single drug utilization so as to reduce the toxic and side effects of the drug and reduce harm on the human body, the economic burden of a patient is further reduced through mixed utilization, and the compound drug performs a better effect than single utilization.

The invention relates to a traditional Chinese medicine formula for treating lung and kidney qi deficiency syndrome of chronic obstructive pulmonary disease (COPD). The problem of the medicine for treating the lung and kidney qi deficiency patient in the stable period of COPD is effectively solved. The traditional Chinese medicine formula comprises 6-20mg ginseng extract, 10-100mg icariin, 1-8mg paeonol, 0.5-6mg nobiletin, 5-20mg paeoniflorin, 3-12mg schisandrin b, 1-8mg peimine, 5-20mg hesperidin, 3-12mg astragalus polysaccharide and 1-8mg astragaloside. The traditional Chinese medicine formula provided by the invention can be effectively applied to the treatment for the lung and kidney qi deficiency syndrome of chronic obstructive pulmonary disease and the preparation of the preparationthereof. Repeated test proves that the traditional Chinese medicine formula is capable of improving COPD lung function, relieving lung tissue pathological injury and inflammatory cell infiltration andimproving inflammatory response and oxidative stress and can be used as long-term used medicine in the stable period for treating COPD. The traditional Chinese medicine formula has the advantages ofreasonable composition, scientific method, high innovation, accurate curative effect and obvious economic and social benefits.

The invention discloses a face cream for repairing skin damaged by ultraviolet rays, which mainly comprises 1-3% of seabuckthorn oil, 1-2% of scallop polypeptide, 0.5-1.2% of nobiletin, 1-2.5% of aloe extract, and 5% of emollient ‑10%, moisturizing agent 2‑7%, emulsifier 2‑5% and other ingredients. The combined use of the composition of the present invention achieves full effect by absorbing ultraviolet rays, increasing oxidase content, scavenging free radicals and superoxide anions, inhibiting tyrosinase activity, promoting skin metabolism, nourishing skin, and moisturizing and locking water. The role of skin care makes the product have whitening, moisturizing and anti-aging effects. In addition, the face cream of the present invention is mild and non-irritating, has high safety, can effectively inhibit allergies caused by ultraviolet rays, repair damaged skin, strengthen protein tissue structure, and make skin firm and fair, and maintain vitality.

The invention discloses a fresh keeping agent for green bell peppers, and belongs to the technical field of fresh keeping agents. The fresh keeping agent mainly consists of osmunda japonica thunb polysaccharide, nobiletin, carboxymethyl cellulose, konjac powder, tea polyphenols, calcium chloride and water. The nobiletin, the osmunda japonica thunb polysaccharide and the tea polyphenols all have the effects of restraining bacteria and resisting oxidation, and the carboxymethyl cellulose and the konjac powder both have favorable film formation, so that when the natural fresh keeping agents are combined together, the storage period of the green bell peppers can be prolonged. The fresh keeping agent only needs to be uniformly mixed when being used, after the fresh keeping agent is cooled, thegreen bell peppers are immersed into the composite fresh keeping agent for about 30s, then the immersed green bell peppers are taken out, and then the taken-out green bell peppers are aired at room temperature. The operation is simple, convenient, natural and environmentally-friendly. Compared with an ordinary fresh keeping agent for fruits and vegetables, the fresh keeping agent disclosed by theinvention can obviously reduce the respiratory intensity and the weight loss rate of the green bell peppers during storage, reduce losses of vitamin C and chlorophyll, control increase of malondialdehyde, and restrain occurrence of rot, and the green bell peppers can be stored for 40 days under the storage condition of 10 +/-1 DEG C.

The invention discloses a process for preparing nobiletin based on multiple solvents. Any one or more of dried tangerine or orange peel (orange peel), fruits of immature citrons, fructus aurantii (bitter oranges) and shaddock peel are adopted as raw materials, the raw materials are smashed into coarse powder firstly, then methyl alcohol or ethyl alcohol with the volume concentration of 50%-95% is adopted as a solvent, and extraction is conducted through a percolation method or a reflux extraction method or an impregnation method; after an extracting solution is collected, vacuum concentration is conducted, and extract without the alcohol taste is obtained; the extract obtained after alcohol extraction is chloroformed with an organic solvent or extracted with ethyl acetate firstly, extractant is concentrated and then repeatedly extracted with a ternary two-phase solvent system formed by acetone, petroleum ether and water, the lower layer of the extractant which is repeatedly extracted is concentrated, coarsely crystallized and repeatedly recrystallized, and nobiletin crystals with the purity of 98% or above are obtained. The obtained nobiletin can be used for quality control over national standard substances/medicinal materials and decoction pieces or quality monitoring on Chinese patent medicine, and compared with the prior art, the process has the advantages of being simple in technological process, practical, low in cost, high in purity and yield and suitable for industrial production.

The invention relates to a natural pharmaceutical composition, preparation method thereof and the usage in preparing an attenuated synergistic medicament in cancer chemotherapy, wherein the natural pharmaceutical composition consists of decoction condensed extract product of dried orange peel and terminalia, nobiletin and 70% ethanol extract product of terminalia in a proportion of (2-6):(3-6):(5-8). TheThe composition has good curative effect to cancer chemotherapy related diarrhoea and increases effect to cancer chemotherapy.

The invention discloses application of nobiletin in preparation or screening of drugs for treating diabetes and cardiomyopathy. Whole animal level experiments prove that the nobiletin has effect of improving diabetes and cardiomyopathy. The dosage of the nobiletin dependently improves heart functions of patients with the diabetes and cardiomyopathy, and myocardial remodeling such as myocardial hypertrophy and myocardial fibrosis is reversed obviously.

The invention relates to a natural pharmaceutical composition, and a preparation method and application thereof. The natural pharmaceutical composition is composed of Plantain seed/dried orange peel water-decocted concentrated extract, Plantain seed polysaccharide and nobiletin in a ratio of (3-7):(1-5):(5-8). The composition has excellent curative effects on diarrhoea related to cancer chemotherapy, and meanwhile, has a synergistic action on cancer chemotherapy.

The invention belongs to the field of pharmaceutical preparations, relates to a quality control method of Huoxiang Zhengqi oral liquid, and in particular relates to a method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by an HPLC method. The invention provides a method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by an HPLC method. According to the HPLC method, a chromatographic column is adopted, and a silica gel matrix is taken as a filler, and a solution A and a solution B are mixed according to a certain proportion to be taken as a mobile phase for elution; the solution A is acetonitrile, and the solution B is phosphoric acid water; and the components comprise one or more of licocoumarin, isoliquiritigenin, apigenin liquiritin, naringin, liquiritin and nobiletin. The method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by the HPLC method is simple, sensitive and high in accuracy, can make up the defect that the prior art is not sufficient enough to explain the content of the components in the Huoxiang Zhengqi oral liquid, and effectively ensures the quality of the Huoxiang Zhengqi oral liquid.