21 results about "Nobiletin" patented technology

The invention relates to a beauty and skin care enzyme and a preparation method thereof. According to the invention, fruits and traditional Chinese medicines are used as main fermentation raw materials, the fruits and the traditional Chinese medicines are crushed and mixed with fermentation strains, and active substances in each raw material are effectively retained through two-stage fermentation, filtration separation and freeze-drying treatment, SOD and sea buckthorn flavone contained in sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis, and the sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis. Salidroside contained in the rhodiola rosea has the skin care effects of resisting light aging and fatigue, astragalus polysaccharide and astragaloside contained in the astragalus membranaceus can repair a barrier and enhance immunity, ophiopogonin and polysaccharide contained in the radix ophiopogonis have the effects of preserving moisture and resisting saccharification, and hesperidin and nobiletin contained in the pericarpium citri reticulatae have the effects of resisting oxidation and adjusting grease secretion. The obtained compound enzyme has the effects of resisting oxidation, resisting inflammation and preserving moisture, has activity, stability and transdermal efficiency, and can be used in skin care products such as anti-oxidation essence, repair masks, late-up cream and the like.

The present invention belongs to the technical field of semen cryopreservation, and more specifically, relates to sheep semen freezing solution and semen preservation method. The sheep semen freezing solution components of the present invention include Tris, citric acid, fructose, lactose, trehalose, penicillin, streptomycin, soy lecithin, nobiletin, glycerol and water. By using a specific content of nobiletin as an effective antioxidant component of a sheep sperm cryopreservative, it plays an antioxidant role when the sperm is frozen, making the sperm more stable during the cryopreservation process. After adding nobiletin, the vitality and motility of sheep sperm after freezing and thawing can be significantly improved. It effectively reduces the oxidative stress damage of sperm during the freezing process and improves the antioxidant capacity of sperm.

The invention relates to a spleen-tonifying, appetite-stimulating and digestion-promoting traditional Chinese medicine preparation UPLC detection method and a fingerprint spectrum, and belongs to the technical field of medicine detection. The invention aims to solve the technical problems that the existing quality control method of the spleen-tonifying, appetite-promoting and digestion-promoting pills lacks comprehensive and objective analysis on the spleen-tonifying, appetite-promoting and digestion-promoting pills, and the problems of low precision, poor reproducibility, low efficiency and the like during detection of related components in the prior art are solved. According to the technical scheme, the UPLC detection method is characterized in that a mixed standard substance of gallic acid, 5-hydroxymethylfurfural, chlorogenic acid, sinapine thiocyanate, sinapic acid, hyperoside, 3, 6-dimustard acyl sucrose, hesperidin, naringenin, nobiletin and hesperetin is taken as a contrast, an acetonitrile-0. 1% formic acid aqueous solution is taken as a mobile phase, gradient elution is carried out, and the content of chlorogenic acid in the sample is detected. The qualitative and quantitative detection of the effective components of the spleen-tonifying, appetite-promoting and digestion-promoting pill is realized.

The invention discloses a method for identifying Xinhui pericarpium citri reticulatae and pericarpium citri reticulatae, which is characterized in that four active ingredients including hesperidin, nobiletin, tangeretin and 2-methylamino methyl benzoate are simultaneously detected through high performance liquid chromatography, and distinguishing is realized according to detection data. The method comprises the following specific steps: preparing a mixed reference solution and a test solution, extracting a test with methanol, performing ultrasonic treatment, and filtering, specific chromatographic conditions are adopted for detection, the detection wavelength of the hesperidin is 283nm, and the detection wavelengths of the other three components are 330nm. Test results show that the contents of the four active ingredients in the Xinhui pericarpium citri reticulatae are high, the content of methyl 2-methylaminobenzoate is not lower than 0.020%, the ratio of the total amount of nobiletin and tangeretin to hesperidin is not lower than 0.17, and related indexes of the pericarpium citri reticulatae are remarkably low. The method is efficient, accurate, stable and reliable, and provides technical support for standardizing market circulation, guaranteeing product quality and promoting standardized development of the Xinhui pericarpium citri reticulatae industry.

The invention provides application of a TRPC6 / TRPM4 molecular sensory artificial intelligence biosensor in anti-virus pungent-warm key quality attribute identification of a negundo chastetree prevention mixture, and belongs to the technical field of traditional Chinese medicine analysis. According to the application, the medicinal components of the negundo chastetree prevention mixture entering blood and target organs are determined through molecular biology, a TRPC6 / TRPM4 double-target receptor is determined through a biosensor and a UPLC-MS (Ultra Performance Liquid Chromatography-Mass Spectrometry) technology, then the pungent and warm components of the negundo chastetree prevention mixture are determined, and the property and flavor key quality attributes of the negundo chastetree prevention mixture are determined by comparing the medicinal components with the pungent and warm components. In addition, nobiletin in key quality attributes of properties and flavors of the negundo chastetree fruit and prevention mixture has good anti-influenza virus activity, and can be used for preparing drugs for treating anti-influenza A H1N1 viruses.

The invention relates to the technical field of traditional Chinese medicine research, in particular to a quality marker for preventing and treating kidney-yang deficiency of a medicinal and edible formula composition as well as a preparation method and application of the quality marker. The medicinal and edible formula composition is prepared from the following raw materials in parts by weight: 1-2 parts of cortex cinnamomi, 6-8 parts of fructus momordicae, 2-4 parts of pericarpium citri reticulatae, 2-4 parts of rhizoma zingiberis, 8-10 parts of radix ginseng, 29-31 parts of radix astragali, 4-6 parts of radix glycyrrhizae preparata and 14-16 parts of poria cocos. The mass markers comprise [6]-isoshogaol, 3, 5, 6, 7, 8, 3 ', 4'-heptamethoxyl flavone, 1-dehydrogenation-[8]-zingiberendione, 6-methyl zingiberendiol, nobiletin, peterolide and the like. The ginseng formula for regulating the middle warmer is reasonably added and subtracted, so that the traditional Chinese medicine composition is applied to prevention and treatment of the kidney-yang deficiency syndrome, and a new application prospect is given to the classic famous formula. Meanwhile, on the basis of spectrum-effect association research, a quality marker group associated with the kidney-yang deficiency effect is screened and established for the first time, and scientific, accurate, stable and controllable product quality of the medicinal and edible formula composition can be achieved.

The inventors have found that quercetin and genistein, individually, suppress the expression of some aging-related genes while promoting the expression of other aging-related genes. Therefore, the main objective was to maintain the repressive effect on some aging-related genes that quercetin, genistein, and their glycosides may have, while suppressing the effect of promoting the expression of other aging-related genes. [Solution] The above problem can be solved by combining two or three components selected from the group consisting of component (i): quercetin and / or its glycosides, component (ii): genistein and / or its glycosides, and component (iii): nobiletin and / or its glycosides.

The invention discloses DHA-nobiletin-EGCG-loaded pectin-based gel soft sweets and a preparation method and application thereof.The preparation method comprises the following steps that DHA-nobiletin-EGCG and calcium ion cross-linked pectin-pea protein Maillard conjugate hydrogel are prepared and mixed, high-speed shearing and emulsifying are conducted under the ice bath condition, and the DHA-nobiletin-EGCG-loaded pectin-based gel soft sweets are obtained; the DHA-nobiletin-EGCG (epigallocatechin gallate) loaded emulsion gel is prepared; and adding glycosyl-pectin-gelatin mixed gel, pouring into a mold, and drying to obtain the DHA-nobiletin-EGCG loaded pectin-based gel soft sweets. The DHA-nobiletin-EGCG loaded pectin-based gel soft sweets disclosed by the invention are uniform and stable in texture, have excellent gel strength, elasticity and chewiness, not only can improve the bioavailability of DHA, but also can realize slow release and targeted delivery of EGCG and nobiletin in intestinal tracts.

The application belongs to the technical field of pharmaceutical preparations, and particularly relates to a liposome of atenolol, a preparation method and a preparation. The liposome of atenolol comprises the following components: atenolol, soybean phospholipid, cholesterol, nobiletin and sesbania gum. By optimizing the excipients required in the preparation of the liposome of atenolol, the defects of low drug loading capacity of the liposome of atenolol in the prior art are overcome, the problem of low delivery efficiency of atenolol caused by low drug loading capacity of the liposome of atenolol in the prior art is solved, and the liposome of atenolol is further expanded to be prepared into a preparation. Compared with the prior art, the preparation has higher stability, and the content of related substances is lower than that of a commercial preparation.

The inventors' primary objective was to provide a technology that suppresses inflammation, particularly in aging tissues. [Solution] The above problem can be solved by a composition containing at least one selected from the group consisting of genistein, quercetin, fisetin, nobiletin, kaempferol, apigenin, and naringenin, and glycosides thereof.

The invention relates to the technical field of biological medicine, in particular to application of nobiletin in prevention and treatment of ionizing radiation damage. The nobiletin provided by the invention is extracted from plants, is of a natural flavonoid structure, has high compatibility with endogenous metabolites of a human body, and has good biocompatibility. Animal experiments prove that when the medicine is administrated at the optimal effective dose (200mg / kg) and 12 hours before irradiation, no obvious toxic reaction is shown, the immunogenicity is low, the medicine is suitable for long-term or conventional radiation protection application, the common problems of adverse reaction and accumulated toxicity in synthesis of protective medicines are solved, and meanwhile, the medicine can be in the optimal action state when being exposed to radiation, so that the medicine can be applied to radiation protection for a long time. The survival time of irradiated mice is remarkably prolonged, acute radiation injury pathological symptoms such as intestinal edema and villus shortening are effectively improved, intestinal crypt regeneration and stem cell proliferation are promoted, and reliable radiation protection efficiency is shown.

The application belongs to the technical field of content detection, and discloses a kind of HPLC detection methods for simultaneously determining the content of multiple components in semen Cannabis capsules.The detection method includes mixing reference solution, chrysophanol and methanol to obtain mixed reference solution.The reference solution includes physcion solution, magnolol solution, houpo chrysophanol solution, emodin solution, rhein solution, nobiletin solution, aloe emodin solution, hesperidin solution and paeoniflorin solution;The contents of semen Cannabis capsules are mixed with methanol, and the test solution is obtained after heating and refluxing;The mixed reference solution and test solution are respectively sucked and injected into high performance liquid chromatograph.The detection method can simultaneously determine the content of multiple index components, can comprehensively reflect the quality of the preparation, and the test method has excellent precision, repeatability, stability and high sample recovery rate.

To provide a food and drink composition which has an excellent effect of promoting secretion of brain-derived neurotrophic factor (BDNF) from small intestinal endocrine cells from safe food materials.SOLUTION: There is provided a food and drink composition for promoting BDNF secretion from small intestinal endocrine cells containing 4'-demethylnobiletin as an active ingredient. The food and drink composition of the present disclosure may have an effect of reducing stress or depression in daily life, and alleviating anxiety, an effect of increasing vitality, energy or motivation, and / or an effect of reducing stomach discomfort or constipation in daily life. Since 4'-demethylnobiletin can be obtained by conversion from nobiletin of a citrus peel component by a koji mold fermentation method, the food and drink composition of the present disclosure is highly safe and has no concerns about side effects.SELECTED DRAWING: Figure 2

The invention discloses a whole-process quality transmission quality control method of a mixture capable of tonifying qi, soothing nerves and eliminating diseases. The method comprises the following steps: respectively preparing a test sample in four process stages of decoction, concentration, centrifugation and sterilization, measuring seven physical parameters including pH, refractive index, dynamic viscosity, conductivity, total amount of soluble solids, solid content and density, and constructing a physical fingerprint spectrum; the method comprises the following steps: determining the contents of nine index components including salidroside, caffeic acid, ferulic acid, specnuezhenide, naringin, hesperidin, rosmarinic acid, formononin and nobiletin by adopting an HPLC (High Performance Liquid Chromatography) method, establishing a chemical fingerprint spectrum, and calculating the transfer rate of each component in each process stage; the correlation between the physical parameters and the component transfer rate is analyzed through the matrix hotspot map, and common peaks are identified to calculate the similarity. A multi-index evaluation mode of physical fingerprint, chemical fingerprint and multi-component content determination is established, the influence of the preparation process on the overall quality of the traditional Chinese medicine can be systematically evaluated, and a scientific basis is provided for quality control in the traditional Chinese medicine production process.

The present invention belongs to the field of analysis of traditional Chinese medicine preparations, and specifically relates to a method for determining the contents of seven active ingredients in the classic ancient prescription Xiangsha Liujunzi Decoction. This method utilizes high-performance liquid chromatography (HPLC) under the same experimental conditions to simultaneously separate and determine the contents of liquiritin, apigenin, naringin, hesperidin, nobiletin, costunolide, and dehydrocostuslactone in Xiangsha Liujunzi Decoction. Experimental results demonstrate that the HPLC method established herein for simultaneously determining the contents of multiple different index components in a preparation is simple, rapid, accurate, reliable, and exhibits good repeatability and stability, making it suitable for use as a quality control method for the preparation.

The invention relates to a pharmaceutical composition for treating chronic obstructive pulmonary disease and application of the pharmaceutical composition. The pharmaceutical composition is prepared from astragaloside, nobiletin and icariin. The composition disclosed by the invention is rich in raw materials, has the effects of improving airflow limitation, resisting inflammation, resisting oxidation and the like, can be effectively used for treating the chronic obstructive pulmonary disease, and has relatively high economic and social benefits.

This invention relates to the field of traditional Chinese medicine component analysis technology, specifically to the fingerprint spectrum of Juanxiao tablets and its establishment method. The method for establishing the fingerprint spectrum of Juanxiao tablets includes: using isoharonidin, isovitexin, rutin, hesperidin, lemon balm glycoside, aloe-emodin, nobiletin, and citrus reticulataein as reference standards, and Juanxiao tablets as the test sample; using HPLC to detect the reference standards and the test sample, and then comparing and analyzing them to determine the common characteristic peaks of Juanxiao tablets; wherein the HPLC conditions include: a C18 column, mobile phase A as a nitrile solvent, mobile phase B as a 0.05-0.15% phosphoric acid solution, and gradient elution. This method can effectively detect characteristic peaks, reduce the drying of impurity peaks, and the obtained fingerprint spectrum has high resolution and good peak shape. All characteristic peaks are baseline separated, enabling a more comprehensive and accurate evaluation of the quality of Juanxiao tablets.

The invention belongs to the field of pharmaceutical compositions, and relates to an anti-muscle aging composition, in particular to a composition for promoting muscle generation and application thereof. The composition comprises nobiletin and milk-derived protein, and intervention of the composition can help to promote muscle generation, improve muscle exercise ability, delay muscle atrophy and inhibit muscle aging. The composition has both functionality and nutrition, and shows a wide application prospect in the aspects of improving muscle functions and athletic ability.

Compositions and methods for improving or regulating GLP-1 levels, or increasing GLP-1 receptors, or reducing GLP-1 degradation, or stimulating GLP-1 secretion in a subject. The composition comprises two or more of the following substances: an effective amount of dihydroberberine (DHB) or berberine, a physiologically acceptable salt, polymer, ester, acid thereof; gymnema sylvestre or gymnemic acid; cinnamon or ceylon cinnamon; nobiletin; or the composition comprises an effective amount of dihydroberberine (DHB), a physiologically acceptable salt, polymer, ester, acid, and one or more of the following substances: β-aminoisobutyric acid, a physiologically acceptable salt, ester; 3-hydroxy-3-methylbutanoic acid, a physiologically acceptable salt, ester; tetrahydrocurcumin, a physiologically acceptable salt, acid, ester; tributyrin; berberine; resveratrol; rhodiola rosea; cinnamon; fenugreek; olive leaf extract; grain of paradise; fruit-vegetable powder; whey protein; or arginine.

The application belongs to the field of pharmaceutical compositions, and relates to an anti-muscle aging composition, in particular to a composition for promoting muscle generation and application thereof. The composition comprises nobiletin and milk-derived protein, and the composition can help promote muscle generation, improve muscle movement ability, delay muscle atrophy and inhibit muscle aging. The composition has both functionality and nutrition, and has broad application prospects in improving muscle function and movement ability.

The invention discloses a Huyou peel exosome drug-loaded targeting preparation as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method specifically comprises the following steps: (1) carrying out tissue disruption on grapefruit peel, carrying out high-speed centrifugation to remove disrupted tissues and cells, carrying out ultra-high-speed centrifugation to collect an exosome crude extract, and carrying out sucrose density gradient centrifugation to purify grapefruit peel exosomes; (2) dissolving nobiletin in ethanol, dissolving the citrus grandis peel exosome in a phosphate buffer solution, mixing, stirring, and carrying out sucrose density gradient centrifugal separation on the citrus grandis peel exosome loaded with nobiletin; and (3) resuspending in a phosphate buffer solution, and freeze-drying to obtain the product. According to the invention, the anti-inflammatory effect of the grapefruit exosome and the in-vivo plasmic reticulum positioning of the grapefruit exosome are utilized to improve the effect of the nobiletin in targeted inhibition of the STING pathway to treat inflammation, and pneumonia is treated through inhalation administration.