665 results about "Xanthonoid" patented technology

The invention relates to a flavonoid ester. This flavonoid ester results from the reaction product of at least one flavonoid selected from the group consisting of a flavonoid with a ketone group in the 4-position, a salt, ester or ether of such a flavonoid, and a C-heteroside and/or O-heteroside derivative of such a flavonoid, with the proviso that this flavonoid contains at least one free alcohol group, with an organic monoacid having from 3 to 30 carbon atoms. These flavonoid esters constitute useful active principles for the manufacture of cosmetic, dermopharmaceutical, pharmaceutical, dietetic or agri-foodstuff compositions.

Flavonoids, especially luteolin, are shown to be effective against insulin dependent (Type I) and insulin independent (Type II) diabetes mellitus. It is demonstrated that luteolin works in mammals by binding and blocking the K_v1.3 potassium channel of T-cell and Beta cells. Antidiabetic and anti-autoimmune compounds can be selected by measuring their ability to bind to and block the K_v1.3 channel.

The invention relates to the technical field of preparation of extracting agents and deep-eutectic solvents, and relates to application of an extracting agent and a deep-eutectic solvent in determining of effective ingredients in traditional Chinese medicine, in particular to application in determining of flavonoid ingredient in radix scutellariae, and application of an extracting agent and a deep-eutectic solvent in determining of effective ingredients of baicalin, baicalein, wogonoside, wogonin and scutellarin in radix scutellariae. The application is characterized in that the radix scutellariae is used as the raw material, the deep-eutectic solvent, especially choline deep-eutectic solvent, is used as the extracting agent, the water is used as a thinner for reducing the system viscosity, and the flavonoid compound is forcibly extracted under the ultrasonic condition. An extracting method has the advantages that the extracting efficiency of the flavonoid ingredient is higher, the extracting conditions are moderate, and the operation is simple and convenient. The extracting agent has the characteristics of no burning explosion and volatizing, small loss, low energy consumption andthe like. The extracting method is also suitable for extracting the flavonoid-containing traditional Chinese medicines, such as radix astragali seu hedysari, liquorice root, radix puerariae and radixsophorae flavescentis.

The invention discloses a buckwheat sprout seedling dish and a preparation method thereof, belonging to the field of foods. The preparation method of the buckwheat sprout seedling dish comprises the steps of seed selection, sterilization, seed soaking, sowing, sprouting, harvesting and the like, and the water content of the obtained buckwheat sprout seedling dish is 80% to 90%. In the invention, the buckwheat sprout seedling dish made by buckwheat after sprouting has the savory taste of buckwheat sprouts and is rich in flavonoid compounds, such as rutin and the like, and 18 kinds of amino acids necessary to a human body simultaneously, the mineral content of the buckwheat sprout seedling dish is higher than that of common buckwheat kernels, the activity of nutritional ingredients is reinforced, and the nutritional ingredients are easy for the human body to absorb; by eating the buckwheat sprout seedling dish frequently, the immunity of the human body can be enhanced, and the health of the human body is strengthened.

The invention discloses a method for separating a flavone compound from hericium erinaceus by applying a high-speed countercurrent chromatography. The method comprises the following steps: 1) subjecting hericium erinaceus fermented mycelia to extraction by adopting high-purity ethanol, then carrying out filtration, carrying out pressure-reducing concentration so as to recover ethanol, and carrying out extraction with petroleum ether and ethyl acetate so as to obtain a crude extract; 2) mixing chloroform, dichloromethane, methanol and water according to a volume ratio of 4: 2: 3: 2 so as to obtain a mixed solution used as a two-phase solvent system, and carrying out standing and stratifying, wherein the upper phase is used as a stationary phase, and the lower phase is used as a mobile phase; and 3) carrying out separation through the high-speed countercurrent chromatography so as to obtain the flavone compound. The method is simple and convenient, has good reproducibility, can be used for large-scale preparation of compounds, and lays a material foundation for further research on activity.

The invention relates to a method for extraction and separation of xanthophyll and flavones in marigold, and belongs to the technical field of extraction of the xanthophyll and flavonoids compounds. According to the method, marigold petal powder is added into an isopropanol-potassium hydroxide two-aqueous-phase system for one-step extraction to separate an upper phase and a lower phase, and a final product is prepared by dealkalization, filtration and evaporation. The method greatly simplifies the process of extraction and separation of the xanthophyll and the flavones, and overcomes the defects of large use amount of organic solvents, high material consumption and high energy consumption. The extraction rate of the xanthophyll in the upper phase reaches 86.73%, and the xanthophyll ester saponification rate reaches 93.98%, and the extraction rate of the flavonoids compounds in the lower phase reaches 78.31%. The method is easy to operate, and green, can realize simultaneous xanthophyll saponification and extraction, and can be used for effective separation of the flavonoids compounds.

The invention provides a method for extracting propolis by simulative biological fluid bed supercritical CO2 fluid, which comprises that: bran (or rice bran and the like) taken as biomembrane carrier and original gum particles after physical treatment simulate a fluid bed in an extraction kettle to perform gridded filling, the supercritical CO2 fluid is used for extraction, and the lowest speed of a granular bed converting from a stationary state to fluidization is regulated by regulating temperature, pressure and flow rate of the fluid in the extraction process so as to reach the critical fluidization state; therefore, high-quality and high-yield propolis extract is obtained through a cyclic process of fluidization diffusion, dissolution and separation between granules and the fluid. The method improves the yield of the propolis extract, reduces the cost, and extracts nearly all terpene compounds and 40 to 50 percent of flavonoid compounds in the propolis at the same time; and products do not have organic solvent.

A process for preparing a rutin-enriched composition from plant biomass comprises extraction with an aqueous solution, and precipitation. An enzyme preparation, such as naringinase, is used for the transformation of rutin to higher value compositions containing increased proportions of isoquercitrin and quercetin.

Disclosed is an improved method for preparing the isoflavonoid compound (+/−)-equol, the method comprising reducing an organic diester of the isoflavone daidzein under hydrogen-transfer conditions using palladium hydroxide catalyst.

The invention discloses an extract of smilax glabra saponins and flavonoid and a preparation method thereof. The preparation method comprises the steps of (1) smilax glabra medical materials are dried in vacuum, crushed and hydrolyzed by enzyme; and (2) smilax glabra saponins and flavonoid compound are extracted with ethanol under the auxiliary function of ultrasonic in inverse flow and condensed; the condensed compound is degreased with petroleum ether and extracted with chloroform and ethyl acetate; after the solvent is recovered, the solvent is dried in vacuum to obtain the extract of smilax glabra saponins and flavonoid which contains 30-80 percent of flavonoid compound. The obtained smilax glabra saponins extract contains 20-70 percent of saponins compound. The method utilizes composite plant extracting enzyme to pretreat the smilax glabra medical materials and extract the smilax glabra saponins and flavonoid compound with ultrasonic auxiliary extract technology, has high utilization degree of the medical materials and high yield of product, consumes low energy and is convenient to operate. The extract of smilax glabra saponins and flavonoid is widely applied to the field of medical materials, midbody and health-care food and has wide market foreground.

The invention belongs to the technical field of biological extraction and particularly relates to a method for deep eutectic solvent extraction and macroporous resin separation and purification of burdock leaf total flavonoids. The extraction solvent in the method consists of the deep eutectic solvent with water content of 0 to 50 percent, wherein the deep eutectic solvent is prepared by reactingcholine chloride with one of ethylene glycol, glycerol, triethanolamine, levulinic acid and malonic acid. The deep eutectic solvent has unique physicochemical properties of greenness, no pollution, low vapor pressure, no toxicity, biodegradability, good solubility and the like; in addition, the deep eutectic solvent has a good extraction effect on flavonoid compounds. According to the method disclosed by the invention, by adopting the method for ultrasonic assisted green novel deep eutectic solvent extraction and the macroporous resin separation and purification of the burdock leaf total flavonoids, the deep eutectic solvent extracting solution can be directly used for separation and purification of macroporous resin. The method has the characteristics of simple operation, low cost, greenness and environment friendliness, and provides a novel idea for extraction, separation and enrichment of burdock leaves.

The invention provides a yoghourt containing flavonoid compounds and a production method for the same. The production method comprises the following steps of: pre-treating the flavonoid compounds; adding the pre-treated flavonoid compounds in milk, or adequately dissolving the pre-treated flavonoid compounds and milk powder in water, and preparing fermentation substrate solution; and producing the yoghourt after homogenizing and sterilizing. According to the production method disclosed by the invention, the flavonoid compounds are added in the yoghourt after a special pre-treatment process, so that the product has a certain anti-ageing function because of containing the flavonoid compounds; moreover, the influences of the colour and bitter taste of the natural flavonoid compounds on the yoghourt are furthest eliminated and reduced, so that the quality of the product is ensured; further, via the addition of the flavonoid compounds, a fermentation process can also be promoted.

The invention relates to alpha-L-rhamnosidase Rha1 as well as an expressed gene and an application of the alpha-L-rhamnosidase Rha1. An amino acid sequence is shown in SEQIDNO.2, a nucleotide sequence of the gene coding alpha-L-rhamnosidase Rha1 is shown in SEQIDNO.1. 1.5g/L of rutin is hydrolyzed for 20min by the alpha-L-rhamnosidase Rha1 under the conditions that the dosage of enzyme is 13U/ml, pH value is 6.5 and the temperature is 35 DEG C, and the hydrolyzing efficiency is more than 98%. Flavonoids compounds, such as rutin, aringin and the like can biologically catalyzed and converted by alpha-L-rhamnosidase Rha1 at a low temperature, and the energy consumption is greatly reduced.

The invention relates to a method for preparing onion functional components by using double-stage extraction based on a subcritical water and solvent method, belonging to the technical field of food refine and deep processing. The invention mainly aims at a series of problems about low survival ratio and more destroys of active components, caused by extracting onion oil by using the traditional constant pressure steam distillation method, solvent residue caused by extracting onion oil by using an organic solvent extraction method, high cost of supercritical extraction, difficult large-scale industrialization production and the like. In the method, with the onions or processed by-products (onionskins, leftovers and the like) thereof as raw materials, and onion oil and flavonoid compounds are respectively prepared through a low-frequency industrial microwave pre-drying wall-broken technology and subcritical water extraction and ethanol-water solvent two-stage extraction technologies so that the quality of the product and the comprehensive utilization ratio of resources are effectively improved. Therefore, the invention meets the development direction of food nutrition, security and health in China and also opens up a new method for effective utilization of onions.

The invention relates to a pharmaceutical composition having alpha-glucosidase inhibition activity, wherein the pharmaceutical composition comprises a flavone compound and an alpha-glucosidase inhibitor, the flavone compound is at least one selected from a monomer such as baicalein, quercetin, luteolin, baicalein-7-O-glucoside and catechin, an organic salt of the monomer, and an inorganic salt of the monomer, and the alpha-glucosidase inhibitor is at least one selected from a monomer such as acarbose, voglibose and miglitol, an organic salt of the monomer, and an inorganic salt of the monomer. According to the present invention, the pharmaceutical composition can effectively reduce postprandial blood glucose, can inhibit the activity of alpha-glucosidase adopting starch, maltose and sucrose as substrates, and less uses the alpha-glucosidase inhibitor, such that the efficacy can be improved, the side effect of the alpha-glucosidase inhibitor can be effectively reduced, and hypoglycemia and other problems easily caused by drug combination are effectively solved.

The invention discloses a preparation method of an enzymatic grafting based antioxidant silk fibroin membrane, and belongs to the biological technical field of textiles. With the help of tyrosinase, antioxidant flavonoids compounds are grafted onto silk fibroin surface after catalytic oxidation; and by crosslinking of tyrosine and the flavonoids compounds in the enzymatic silk fibroin, the antioxidant properties of the silk fibroin membrane is enhanced. The specific processes include silk fibroin solution preparation, silk fibroin membrane preparation, grafting of the flavonoids compounds on the enzymatic silk fibroin and after treatment of the modified silk fibroin membrane. The silk fibroin membrane prepared by the preparation method has improved antioxidant and antimicrobial properties, and the membrane material mechanical properties also are improved. Compared with a silk fibroin membrane modification method based on chemical crosslinking, the enzymatic modification method is low in energy consumption, high in efficiency, less in pollution and in favour of environmental protection.

The invention relates to a drug for treating purine metabolic disorder disease, provides a flavonoids compound represented by the formula (I) and a modified derivative thereof, and a use in inhibition of xanthine oxidase activity and promotion of uric acid excretion function, wherein R1, R2 and R3 independently represent -H or monosaccharide residues, and R4 represents -H or -OH. In particular, the drug is used for treating purine metabolic disorder disease, and can be used for treating hyperuricemia and related diseases and reducing a individual serum UA level; the drug is used for treating hyperuricemia; the drug is used for treating the related diseases, such as for treating gouty arthritis and arthrophlogosis caused by hyperuricemia, and for treating chronic interstitial nephritis, kidney stones and the like caused by hyperuricemia. The drug has the advantages of natural chemical structure, safety, low toxicity, rapid curative effect and fewer side effects. (I).

The invention relates to an application of a flavonoid compound, the flavonoid compound is 3, 3', 4', 5, 7-pentahydroxyflavone dihydrate, the ELISA and protein fluorescence quenching experiments provethat the compound can effectively inhibit interaction of PD-1/PD-L1 proteins, and has a strong affinity for PD-L1 protein. Cell clone number formation and ELISA detection of IL-2 secretion assay prove that the compound can effectively enhance the tumor killing activity of T lymphocyte Jurkat cells against MDA-MB-231 and NCI-H460 tumor cells as well as the expression level of IL-2, and then reverse T cellular immunosuppression. The research results of the application provide a drug for tumor immunotherapy targeting the PD-1/PD-L1 immunological test site.

The invention discloses a gene related to flavone composite in radix scutellariae. The gene includes one or several in flavone synthetase gene with a sequence shown in SEQ ID No.1, flavone 6-site hydroxylase gene with a sequence shown in SEQ ID No.3 and flavone 8-site hydroxylase gene with a sequence shown in SEQ ID No.5. The invention further provides primer composite for amplifying the gene, protein encoded by the gene, a recombinant vector, host cell or transgenic cell line containing the gene and application thereof. The protein encoded by the gene disclosed by the invention can participate in synthesizing baicalein and wogonin and catalyzing synthesis and hydroxylation reaction of flavone matters of the similar structures, can provide very good basis for producing effective active matters, provides theoretical basis for producing baicalein, wogonin and aglycone of the baicalein and the wogonin in a large scale and establishes solid basis for industrially producing other related flavonoid compound.

The invention relates to compound beauty-maintaining jerusalem artichoke aloe skin chewable tablets and a preparation method thereof, belonging to the technical field of food processing. The chewable tablets are prepared by the following steps: taking jerusalem artichoke powder, aloe skin powder and rose petal powder as main raw materials, xylitol, mannitol, citric acid and the like as auxiliary materials and magnesium stearate as a lubricant according to a certain mass ratio, grinding, mixing, adding a wetting agent to prepare a soft material, pelleting, drying, granulating, tabletting, sterilizing, packaging and the like. The prepared chewable tablets are rich in various bioactive substances such as aloe-emodin, aloin, flavonoids compounds, active polysaccharides, dietary fiber, rose anthocyanins and jerusalem artichoke fructosan, uniform in color, palatable in sourness and sweetness and convenient to carry, have the aroma of roses and the clean aromas of aloes and jerusalem artichoke, have the effects of promoting digestion, diminishing inflammation, preventing decayed teeth and obesity, calming the nerves, activating blood, relieving hypertension, hyperlipidemia and hyperglycemia, resisting oxidation and ageing and improving immunity after being eaten often, and have remarkable beauty-maintaining health-care effects.

The invention discloses a method for efficiently extracting flavonoid from tetrastigma hemsleyanum. The method comprises the steps of: air drying tetrastigma hemsleyanum, mincing, adding water, extracting from an ultrasonic extractor, concentrating the extractive solution to a certain volume, decolorizing with activated carbon, performing liquid extraction with ethyl acetate by using a cavitation suspension extraction and separation device, recovering the ethyl acetate, performing liquid extraction of the aqueous fraction with chloroform, recovering the chloroform, collecting the aqueous fraction, concentrating, purifying with macroporous adsorption resin, eluting with 50% water and ethanol in sequence, collecting the eluent with 50% of ethanol, recovering the solvent, and drying to obtain tetrastigma hemsleyanum extract.

The invention provides a whitening and moistening facial mask and a preparation process thereof. The whitening and moistening facial mask comprises a preparation A and a preparation B, wherein the preparation A is mainly prepared from nicotinamide playing a role in whitening and moistening, vitamin B6, soy isoflavone, hesperidin, liquorice extract, beta-glucan, pure natural jasmine flower extract and pearl extract, wherein the flavone compounds are combined with the vitamin B6 and nicotinamide (B3) to play a role in synergizing and remarkably improving the whitening effect, the liquorice extract and beta-glucan play roles in resisting allergy and radiation, and the jasmine extract and pearl extract enable the facial mask to be mild in performance and have fresh jasmine fragrance; the preparation B is prepared from pure natural anti-aging nutritious supplementary blueberry anthocyanin. The preparation A and the preparation B are packaged separately, so that more scientific application of the facial mask is realized, and the effect of the blueberry anthocyanin can be sufficiently exerted. The whitening and moistening facial mask has scientific formula, mild performance and prominent whitening and moistening effect; moreover, the preparation process is simple, the production cost is low, and batch production can be easily realized.

Rhizoma Drynariae extract (RDE) is used as therapeutical agent or in the preparation for Osteoporosis, which is characterized in containing over 30 percent of total flavonoids. Furthermore naringin contained in flavonoids is more than 30 percent and less than 100 percent. The RDE is applied for the therapy of osteoporosis or used for producing drugs treating osteoporosis. And methods of extracting the same are also related.