461 results about "Xanthonoid" patented technology

The invention discloses a Sarcabomyces lipolytica strain for synthesizing (2S)-naringenin and application of the Sarcabomyces lipolytica strain, and belongs to the technical field of engineering and bioengineering. According to the invention, a key enzyme CHS is subjected to semi-rational transformation, so that the yield of the strain (2S)-naringenin expressing the mutant V196A / T132C is increased by 60%. According to the method, the modified CHS is further integrated into a yeast genome, conversion of p-coumaric acid to (2S)-naringenin is promoted by strengthening a shikimic acid pathway and enhancing supply of cofactors, so that the yield of the (2S)-naringenin reaches 776mg / L after the constructed strain is fermented in a 24-pore plate for 96 hours, and the yield of the constructed strain can reach 8.65 g / L after the strain is fermented in a 5L fermentation tank; the method lays a foundation for the subsequent biosynthesis of flavonoid compounds, and has potential value and significance for the development of synthetic biology.

The invention discloses an application of a CsHCT1 gene in increasing the content of citrus flavonoids, belongs to the technical field of agricultural biological genetic engineering, and provides an application of the CsHCT1 gene in increasing the content of citrus flavonoids, and the nucleotide sequence of the CsHCT1 gene is as shown in SEQ ID NO.1. The invention further discloses an application of the CsHCT1 gene in increasing the content of citrus flavonoids. The invention also discloses a method for improving the flavone compound of the citrus peel by using the CsHCT1 gene. The method comprises the following steps: cloning the citrus CsHCT1 gene; a CsHCT1 overexpression vector is constructed; transforming the overexpression vector to agrobacterium tumefaciens to obtain an agrobacterium tumefaciens liquid; and injecting the CsHCT1 gene into the citrus fruit to obtain the CsHCT1 gene overexpressed citrus fruit. A new choice is provided for increasing the flavonoid compounds in the citrus peel, the content of vicenin-2 and / or hesperidin in the citrus peel is effectively increased, and the application has great application value for cultivating new functional citrus varieties and accelerating the breeding process.

The invention discloses a method for extracting and purifying flavone in litsea coreana through an ultrasonic-assisted eutectic solvent, and belongs to the technical field of green processing of natural products. The invention provides a method for efficiently preparing litsea coreana flavone by integrating computer prediction, green solvent extraction and resin purification technologies. The method comprises the following steps: firstly, calculating and screening a deep eutectic solvent system with the highest affinity with the litsea coreana flavone on the basis of a COSMO-RS theoretical model, then coupling the optimized DES with an ultrasonic-assisted extraction (UAE) technology, and through the synergistic effect of the DES and the UAE technology, remarkably improving the extraction rate of the litsea coreana flavone and furthest keeping the biological activity of a target component; then, a macroporous resin dynamic adsorption-desorption purification process is linked, so that high-selectivity enrichment and impurity removal of the flavonoid compounds in the crude extract are realized, a high-purity product is obtained, and a complete process route from intelligent screening, efficient extraction to precise purification is finally formed.

The invention belongs to the technical field of hair care products, and particularly relates to composite acid gel for scalp care and a preparation method thereof. Salicylic acid, glycolic acid, piroctone azelate olamine salt, echinacea purpurea-lactic acid bacteria yeast, an emulsifier and water are combined, wherein the echinacea purpurea-lactic acid bacteria yeast is prepared by performing enzymolysis on echinacea purpurea through bacillus subtilis protease and then performing aerobic fermentation with lactic acid bacteria. Salicylic acid, glycolic acid and azelaic acid in the composite acid gel are compounded, so that stimulation of salicylic acid is weakened, and the effect of dissolving hair follicle grease is reserved; glycolic acid and azelaic acid are added to accelerate keratin metabolism, regulate sebum secretion and create safe and healthy shielding for the scalp environment, and polysaccharide and flavonoid compounds in the echinacea purpurea-lactic acid bacteria leavening are beneficial to exerting the anti-oxidation and immune regulation effects of the echinacea purpurea-lactic acid bacteria leavening. Therefore, the composite acid gel provided by the invention is green, safe and non-irritant, the preparation method is simple and convenient, and the composite acid gel can be used for inhibiting bacteria and reducing scalp inflammation.

The application discloses a galactosyltransferase mutant and application thereof in preparation of flavonoid compounds, and belongs to the technical field of enzymology. The application provides a UDP-galactosyltransferase mutant, which is obtained by site-directed mutation of one amino acid in the amino acid sequence of wild-type galactosyltransferase VcUFGT into alanine (A). The effects of a series of mutants H82A, V139A, G141A, P186A, N245A, V282A and S307A are verified in the examples, the mutants can perform enzyme catalysis reaction with quercetin and UDP-galactoside as substrates, and generate flavonoid compound hyperoside, and the specific enzyme activity of the mutants is more than 1.5 times that of the wild type, thereby providing an effective application basis for biosynthesis of flavonoid compounds.

The invention belongs to the field of natural product extraction, and relates to a method for extracting and separating flavonoid compounds from Artemisia chrysantha, which comprises the following steps: carrying out reflux extraction on the dry overground part of Artemisia chrysantha by using 70% ethanol, loading to a balanced D101 macroporous adsorption resin column, sequentially eluting by using water, a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution, and collecting the eluent. Four fractions from Fr. I to Fr. IV are obtained; respectively carrying out polyamide column chromatography separation on Fr.II to Fr.IV, and sequentially eluting with a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution to respectively obtain three fractions; and further purifying the Fr.II-1, the Fr.II-2, the Fr.III-1, the Fr.III-2, the Fr.III-1 and the Fr.III-3 by virtue of a Sephadex LH-20 column, so as to finally obtain seven flavonoid compounds. According to the method, only the ethanol water low-toxicity solvent is used for extraction and preparation, organic solvents with high toxicity are not used, the method is green and environment-friendly, the extraction efficiency is high, the number of extracted compounds is large, chemical components of the artemisia europaea medicinal material are enriched, the resource source of active components is widened, and a foundation is laid for follow-up research.

The invention relates to the technical field of preparation of viscose fibers, in particular to a preparation method of viscose fibers based on wormwood essential oil / paraffin double-layer microcapsules, which comprises the following preparation steps: S1, preparing the wormwood essential oil / paraffin double-layer microcapsules; s2, preparing a microcapsule spinning solution; s3, spinning and forming. According to the invention, the wormwood essential oil is coated in the double-layer microcapsule, so that the natural antibacterial property of the wormwood essential oil is fully exerted. Main active components (such as eucalyptol and flavonoid compounds) of the wormwood essential oil are continuously released through a microcapsule slow release mechanism, and growth of common pathogenic bacteria such as escherichia coli and staphylococcus aureus is remarkably inhibited. Meanwhile, due to the double-layer structure (the polyurethane inner shell and the polyurea outer shell) of the microcapsule, volatilization and oxidation of the wormwood essential oil in the spinning process are effectively avoided, and the long-acting antibacterial effect is ensured.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The invention discloses a new application of isoquercitrin in preparation of a medicine for treating renal interstitial fibrosis, and finds and verifies that the isoquercitrin can be used for effectively treating the renal interstitial fibrosis through experiments for the first time, and the isoquercitrin is a natural flavonoid compound. The isoquercitrin has the advantages of low toxic and side effects, high safety, high effectiveness and the like, in addition, the isoquercitrin is rich in raw material resource and has good social and economic benefits, a brand new thought and strategy are provided for research and development of renal interstitial fibrosis treatment medicines, and the application prospect in the technical field of renal interstitial fibrosis treatment is wide.

The invention relates to the technical field of vegetable planting, in particular to application of nano Mn3O4 in relieving vegetable salt stress and / or increasing the content of vegetable flavonoid compounds. The invention finds that nano Mn3O4 prepared by taking manganese acetate tetrahydrate as a manganese source not only can relieve salt stress of vegetables, but also can improve the content of flavonoid compounds in the vegetables, so that the nutritional value or medicinal value of the vegetables is improved. Furthermore, nano Mn3O4 is sprayed on vegetable leaves under salt stress, so that the salt stress of vegetables is relieved, and the content of flavonoid compounds in the vegetables is increased. According to the method provided by the invention, the physiological indexes of the vegetables are improved, the flavone content is increased, the influence of salt stress on the vegetables is relieved, and the method is a simple, effective and environment-friendly method for relieving the salt stress of the vegetables.

The invention provides an application of dihydroflavonoid compound naringin in promoting proliferation and differentiation of porcine muscle stem cells to form myotubes, which is characterized in that the naringin is added into a DMEM / F12 culture medium containing 15% of fetal calf serum and 1% of double antibodies and a DMEM differential culture medium containing 2% of horse serum and 1% of double antibodies until the final concentration is 70 mu M, and the porcine muscle stem cells are respectively treated in proliferation and differentiation stages, hoechst 33342 staining and immunofluorescence staining methods are used for respectively judging the number of cells in the proliferation stage and the fluorescence area, fluorescence intensity and nuclear fusion rate of Myosin in myotubes formed in the differentiation stage. The relative proliferation multiple of the muscle stem cells treated by adding naringin and the fluorescence area, the fluorescence intensity and the nuclear fusion rate of Myosin in myotubes are obviously higher than those of a blank control group. By adopting the method, proliferation and differentiation of the porcine muscle stem cells can be promoted, and a new thought is provided for maintaining cell dryness and large-scale production of cultured meat.

The invention relates to a preparation method and application of a corn straw beverage, belongs to the technical field of plant resource processing, and solves the technical problem of activity loss caused by easy oxidative degradation of flavonoid compounds in a corn straw water extract. According to the technical scheme, the method comprises the following steps: crushing corn straws, soaking in water, performing heat extraction for multiple times to obtain an extracting solution, performing rough filtration and centrifugation, and concentrating to obtain a water extracting solution; and mixing the water extract, sucralose, citric acid, potassium sorbate and purified water to prepare a beverage, sterilizing and filling. Flavonoid compounds are embedded through a potato starch nanoparticle and beta-cyclodextrin composite carrier, a sodium alginate-chitosan microcapsule double-layer coating and microcapsule jelly particle preparation technology is combined, a gel network is formed by compounding kappa-carrageenan and konjac glucomannan, and the embedding stability and release controllability of active ingredients are improved. The beverage prepared by the method has both herbal flavor and crisp and tender taste of jelly particles, effectively delays degradation of flavonoid compounds, and is suitable for being applied to development of functional plant beverages.

The invention belongs to the technical field of natural product extraction, and particularly relates to a research method for extracting polyphenol and flavonoid compounds in peony petals through an electrochemically-assisted eutectic solvent. The invention provides a research method for extracting polyphenols and flavonoids compounds in peony petals by using an electrochemically-assisted deep eutectic solvent, electrochemical wall breaking and DES regulation are combined for the first time by using a solvent design-electrochemical activation-targeted extraction method, phenol oxidation caused by traditional heating is avoided, and meanwhile, the extraction efficiency of the polyphenols and flavonoids compounds in peony petals is improved. The dissolution of target components is selectively enhanced through electrode reaction, the extraction efficiency of bioactive components in the peony petal powder is effectively improved, and the method has the advantages of greenness, high efficiency, short-time consumption reduction and the like, overcomes the defects in the prior art, and has a good application prospect.

The invention discloses a method for increasing the yield of astaxanthin by utilizing a flavonoid compound, which comprises the following steps: taking phaffia rhodozyma as a fermentation strain, and adding the flavonoid compound for fermentation to produce astaxanthin. Quercetin and baicalein are selected as sources of flavonoid compounds; the concentration of the flavonoid compound mother liquor is 2-5g / L, and the flavonoid compound is added into the fermentation culture medium when the phaffia rhodozyma is fermented for 2-3 days. Flavonoid compounds are added in the fermentation process, so that phaffia rhodozyma cell membrane fluidity is promoted, and the yield of astaxanthin is increased.

The invention discloses a method for synergistically extracting flavonoid compounds in epimedium wushanense by combining a ternary eutectic solvent with in-situ ultrasound, which is characterized in that choline bitartrate-urea-glycerol (Chb: Ure: Gly) is used as the ternary eutectic solvent, and the flavonoid compounds in epimedium wushanense are synergistically extracted by the in-situ ultrasound. The method is high in extraction speed and good in repeatability, solvent consumption can be reduced, operation steps are simplified, and efficiency is improved.

The invention relates to the technical field of daily chemicals, in particular to a cacumen biotae fermentation filtrate, a caviar and cacumen biotae nano-coated slow-release composition, shampoo and application. The fermentation filtrate is prepared from cacumen biotae through a liquid fermentation method; wherein the used strain is bacillus and / or aspergillus. The cacumen biotae fermented filtrate provided by the invention contains flavonoid compounds (such as quercetin) and volatile oil (such as cacumen biotae ketone) which can improve scalp blood circulation, nourish hair follicles and promote healthy growth of hair. The shampoo can inhibit hair follicle inflammation and prevent hair follicle atrophy, thereby reducing alopecia. Tannin components can balance grease secretion of scalp, and hair follicle blockage caused by excessive grease is avoided. The shampoo is particularly friendly to oily scalp and is beneficial to keeping the scalp fresh and cool. Polysaccharide and flavonoid substances in the cacumen biotae can prolong the hair growth period and stimulate the activity of hair follicles, so that new hair regeneration is promoted. The plant extract mildly stimulates scalp and does not damage hair follicle tissues.

The invention belongs to the technical field of biosynthesis of plant secondary metabolites, and particularly relates to a key gene for biosynthesis of large-fruit hawthorn flavonoid compounds and a screening method and application of the key gene. Through combined analysis of metabolome and transcriptome, a key gene combination containing seven structural genes and three transcription factor genes is screened out; the expression of the genes is remarkably positively correlated with the accumulation of a target flavone metabolite [6]-gingerol, and the genes are core factors for regulating and controlling the synthesis of the flavonoid compounds of the big-fruit hawthorns through experimental verification. According to the invention, the key gene for regulating and controlling the synthesis of flavone substances such as [6]-gingerol in the big hawthorn fruit is systematically identified for the first time, and a target spot is provided for analyzing a quality formation mechanism from a molecular level; the gene can be used for molecular marker-assisted breeding so as to cultivate a new variety of large-fruit hawthorn with high flavone content, and also can provide gene resources and technical support for the development of functional food and health care products.

The invention relates to the technical field of extraction and processing of radix puerariae and perilla frutescens, and particularly discloses a compound extract of radix puerariae and perilla frutescens as well as a preparation method and application of the compound extract. The preparation method comprises the following steps: slicing radix puerariae, cleaning with purple perilla, drying, adding water to adjust the water content, adding a leavening agent, and fermenting to a specific pH value to obtain a fermented product; and performing reflux extraction on the fermentation product by using a deep-eutectic solvent to obtain the deep-eutectic solvent extract. Mixing the extract with ethanol, and performing supercritical carbon dioxide extraction to obtain a supercritical carbon dioxide extract. Carrying out water extraction and alcohol precipitation treatment on the extract to obtain a primarily purified extract; and finally, separating by using macroporous resin to obtain the final compound extract of the radix puerariae and the perilla frutescens. According to the method, the extraction rate of flavonoid compounds and other bioactive substances in the compound extract is remarkably increased, so that the compound extract shows excellent effects in the aspects of reducing blood pressure, reducing blood sugar, reducing blood fat and the like, can be used as a daily diet supplement, and is widely applied to the fields of foods, medicines and health-care products.

The invention discloses an extraction method of rose flavonoids, which comprises the following steps: S1, crushing selected dry Hetian rose buds, and storing for later use; s2, accurately weighing 1g of rose powder and putting the rose powder into a conical flask; s3, then, according to the material-liquid ratio of 1: 40, 40% ethanol is used as a solvent, and the materials are poured into a conical flask to be mixed; and S4, finally, soaking in a constant-temperature water area, extracting for 2 hours, and finally, taking supernate for later use. According to the invention, the solid-liquid ratio of 1: 40 ensures that the rose powder is fully infiltrated by an enough solvent (40% ethanol). Under the proportion, the ethanol can be in full contact with target components (such as flavonoid compounds and the like) in the rose powder. Each 1g of rose powder is surrounded by 40ml of solvent, so that target components have more opportunities to be dissolved out of powder particles to enter the solvent, and the extraction completeness can be improved compared with the condition that a feed liquid is relatively small.

The invention belongs to the technical field of medicines, and particularly relates to a flavonoid compound-meglumine co-amorphous substance and a preparation method thereof. The flavonoid compounds comprise baicalein, hesperetin, naringenin, kaempferol, quercetin, isoliquiritigenin and the like, and the co-amorphous substance consists of a single flavonoid compound and meglumine and is prepared by adopting a melting shock cooling method or a rotary evaporation method. The miscibility of the flavonoid compounds and the meglumine is screened through Hansen solubility. The crystal diffraction characteristics, thermodynamic properties and intermolecular interaction of the co-amorphous substance are measured through PXRD, DSC and FTIR, and it is shown that the six flavonoid compounds and meglumine successfully form a co-amorphous system. The solubility and the dissolution rate of the six flavonoid compound-meglumine co-amorphous substance are obviously superior to those of flavonoid bulk drugs, and the physical stability of the formed co-amorphous substance is obviously superior to that of a single amorphous drug, so that the co-amorphous substance shows good patent medicine performance.

The invention relates to the technical field of skin care product preparation, in particular to traditional Chinese medicine skin care cream for repairing skin and a preparation method thereof. The preparation method comprises the following steps: preparing acylated alkyl glucoside; preparing a turpinia arguta extract; preparing a suspension of the naringin and the turpinia arguta extract; and preparing the traditional Chinese medicine skin cream for repairing the skin. According to the method, myristic acid and alkyl glycoside are used for preparing acylated alkyl glycoside, myristic acid and alkyl glycoside are subjected to acylation reaction through lipase Lipozyme435 enzyme, damage to a flavone structure in the microwave extraction process is avoided, after alkyl glycoside acylation, the lipophilicity is remarkably enhanced, and the lipophilicity of alkyl glycoside is remarkably reduced. Due to the enhanced lipophilicity, the alkyl glycoside can more effectively interact with fat-soluble flavonoid compounds, so that the solubility and dispersity of the alkyl glycoside in a fat phase are improved, the oxidation loss of flavonoid substances in the extraction process of the turpinia arguta leaves is reduced, and the content of the flavonoid substances in the turpinia arguta leaf extract is increased.

The invention relates to the technical field of biological medicine, in particular to anti-acne hydrogel and a preparation method thereof. The anti-acne hydrogel is prepared from metal-polyphenol network nanoparticles and a hydrogel base material, the metal-polyphenol network nanoparticles comprise a first polyphenol compound, a second polyphenol compound and metal ions, the first polyphenol compound is a tannic acid compound, and the second polyphenol compound is a flavonoid compound; the hydrogel base material comprises a hydrophilic high-molecular polymer and a cationic polymer. The metal-polyphenol network nano-particles are creatively utilized, the anti-inflammatory and anti-oxidation activity of flavonoid compounds, the convergence and protein binding characteristic of tannin and the antibacterial activity of metal ions are integrated at the same time, and the vicious circle of acne can be intervened at the same time from multiple links of antibacterial, anti-inflammatory, anti-oxidation and keratinization adjustment; and the defect of single action target spot of the existing medicine is overcome.

The invention belongs to the technical field of active substance extraction, and discloses a method for extracting boxthorn leaf total flavonoids through an ultrasonic-assisted deep-eutectic solvent and application of the boxthorn leaf total flavonoids. Heating and mixing the hydrogen bond acceptor and the hydrogen bond donor, stirring to be transparent, and adding water to reduce viscosity to obtain the eutectic solvent; mixing the boxthorn leaf powder with a deep-eutectic solvent, performing ultrasonic extraction and centrifugation, and collecting supernate; adsorbing by using macroporous resin, washing and desorbing to obtain an eluent; and performing vacuum freeze drying to constant weight. According to the method disclosed by the invention, the eutectic solvent with low melting point and strong solubility is selected, the flavonoid compounds in the boxthorn leaves are green and efficiently extracted, and the total flavonoid content of the boxthorn leaves obtained by taking the eutectic solvent as an extraction solvent is 123.94 + / -1.04 mg / g and is far higher than the total flavonoid content of the boxthorn leaves obtained by traditional ethanol extraction by 64.60 + / -0.87 mg / g; the method is good in safety, short in time consumption and high in extraction rate.

The invention provides a culture medium composition for saussurea involucrate tissue culture and application thereof, and belongs to the field of plant tissue culture. On the basis of the MS culture medium, the culture medium composition also comprises the following components: 9 to 11 g / L of fructose, 9 to 11 g / L of glucose, 9 to 11 g / L of sucrose, 5 to 7 g / L of agar, 0.5 to 1.5 mg / L of 2, 4-dichlorphenoxyacetic acid, 0.05 to 0.1 mg / L of naphthylacetic acid, 0.05 to 0.2 mg / L of N-phenyl-N '-1, 2, 3-thiadiazole-5-urea, 400 to 600 mg / L of lactoalbumin hydrolysate, 0.2 to 0.4 g / L of activated carbon and 10 to 25 mg / L of methyl jasmonate. By integrating hormone, functional additives (lactoalbumin hydrolysate and activated carbon) and a nano material (silver / copper doped nano zinc oxide), a multi-level synergistic effect is formed, so that the yield of flavonoid compounds is improved on the basis of improving the callus induction rate of saussurea involucrata.

The invention belongs to the technical field of gene engineering, and particularly relates to a highland barley flavonoid glycosyl transferase gene and application thereof. A novel glycosyl transferase gene HOVUSG6091200 in highland barley is found, and protein expressed by the gene can efficiently catalyze glycosylation modification of various flavonoid compounds, so that flavonoid compound glucoside is prepared, and the health-care value of the highland barley is improved; the gene is also transferred into tobacco, so that the highland barley flavonoid glycosyl transferase gene is also expressed in a tobacco plant, flavonoid glucoside is further generated, and the value of the tobacco plant is improved; the novel gene, the recombinant vector, the recombinant bacteria and the transgenic plant provided by the invention have good application prospects.

The present invention relates to the field of feed, and in particular to a feed additive for preventing diarrhea in young ruminants and its application in liquid feed and solid feed. The composition provided by the present invention comprises 5-10wt.% of flavonoids, 5-10wt.% of plant essential oils and 80-90wt.% of yeast cell wall polysaccharides. The composition of the present invention is formulated with flavonoids, plant essential oils and yeast cell wall polysaccharides, and prevents the occurrence of calf diarrhea by regulating the calf's immune system, intestinal mucosal barrier system and intestinal microbial system, improving the calf's body immunity, enhancing the intestinal mucosal barrier function and promoting the balance of intestinal flora, thereby achieving the purpose of promoting the growth and development of calves and healthy cultivation.

The application discloses a flavonoid compound in eaglewood as well as a preparation method and application of the flavonoid compound, and relates to the technical field of medicines. The application extracts and separates a new compound from natural eaglewood, and the new compound is used in the preparation of anti-osteoporosis drugs. The compound is detected in vitro to obtain an induction effect of the compound on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts, so as to affect the formation of osteoclasts. Meanwhile, the compound can promote the bone development of zebra fish through RT-qPCR detection, which indicates that the compound has anti-osteoporosis activity. The separation method of the compound is simple and easy to operate.

The invention discloses a molecularly imprinted adsorption material taking a metal organic framework as a carrier skeleton as well as a preparation method and application of the molecularly imprinted adsorption material, and belongs to the technical field of adsorption materials. The preparation method comprises the following steps: carrying out hydrothermal reaction on transition metal salt and a phthalic acid ligand to obtain metal organic framework nanoparticles; the preparation method comprises the following steps: adsorbing flavonoid template molecules by metal organic framework nanoparticles, mixing with lysozyme and a disulfide bond reducing agent, performing phase change polymerization, and eluting to remove the flavonoid template molecules after the phase change polymerization is completed, thereby obtaining the molecularly imprinted adsorbing material with abundant imprinting sites and good stability. The adsorbent is good in flavonoid compound adsorption selectivity, high in rate, large in capacity, good in chemical stability, resistant to acid washing, capable of being recycled for multiple times and capable of being widely applied to extraction and separation of flavonoid compounds.

The invention discloses application of Dendrobium huoshanense DhOMT8 gene coded methyltransferase in biosynthesis of a flavonoid compound for regulating gastrointestinal activity, and belongs to the technical field of biology. The nucleotide sequence of the DhOMT8 gene encodes a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO. 2. The methylated flavonoid compound is obtained by a biological synthesis method for carrying out enzyme catalysis by using the DhOMT8 protein in vitro, and a new method is provided for synthesizing the flavonoid compound for regulating gastrointestinal activity.

The invention discloses a method for predicting liquid chromatography retention time of traditional Chinese medicine active ingredients. The method comprises the following steps: detecting retention time of flavonoid compounds or anthraquinone compounds under different chromatographic conditions through a reverse high performance liquid chromatograph; performing structure optimization on the flavonoid compound or the anthraquinone compound; performing molecular descriptor calculation on the optimized compound structure to obtain a molecular descriptor data set; performing genetic algorithm screening on the molecular descriptor data set to obtain characteristic molecular descriptors; combining the characteristic molecule descriptors with the different chromatographic conditions into a complete data set; the complete data set serves as an input variable, retention time serves as an output variable, and a gradient elevator GB algorithm or a random forest method RF is adopted for modeling to obtain a QSRR model; and predicting the retention time of flavonoid or anthraquinone compounds under different chromatographic conditions by using the constructed QSRR model.