131 results about "Xanthonoid" patented technology

The invention belongs to the technical field of biosynthesis of plant secondary metabolites, and particularly relates to a key gene for biosynthesis of large-fruit hawthorn flavonoid compounds and a screening method and application of the key gene. Through combined analysis of metabolome and transcriptome, a key gene combination containing seven structural genes and three transcription factor genes is screened out; the expression of the genes is remarkably positively correlated with the accumulation of a target flavone metabolite [6]-gingerol, and the genes are core factors for regulating and controlling the synthesis of the flavonoid compounds of the big-fruit hawthorns through experimental verification. According to the invention, the key gene for regulating and controlling the synthesis of flavone substances such as [6]-gingerol in the big hawthorn fruit is systematically identified for the first time, and a target spot is provided for analyzing a quality formation mechanism from a molecular level; the gene can be used for molecular marker-assisted breeding so as to cultivate a new variety of large-fruit hawthorn with high flavone content, and also can provide gene resources and technical support for the development of functional food and health care products.

The invention discloses an extraction method of rose flavonoids, which comprises the following steps: S1, crushing selected dry Hetian rose buds, and storing for later use; s2, accurately weighing 1g of rose powder and putting the rose powder into a conical flask; s3, then, according to the material-liquid ratio of 1: 40, 40% ethanol is used as a solvent, and the materials are poured into a conical flask to be mixed; and S4, finally, soaking in a constant-temperature water area, extracting for 2 hours, and finally, taking supernate for later use. According to the invention, the solid-liquid ratio of 1: 40 ensures that the rose powder is fully infiltrated by an enough solvent (40% ethanol). Under the proportion, the ethanol can be in full contact with target components (such as flavonoid compounds and the like) in the rose powder. Each 1g of rose powder is surrounded by 40ml of solvent, so that target components have more opportunities to be dissolved out of powder particles to enter the solvent, and the extraction completeness can be improved compared with the condition that a feed liquid is relatively small.

The invention discloses a method for predicting liquid chromatography retention time of traditional Chinese medicine active ingredients. The method comprises the following steps: detecting retention time of flavonoid compounds or anthraquinone compounds under different chromatographic conditions through a reverse high performance liquid chromatograph; performing structure optimization on the flavonoid compound or the anthraquinone compound; performing molecular descriptor calculation on the optimized compound structure to obtain a molecular descriptor data set; performing genetic algorithm screening on the molecular descriptor data set to obtain characteristic molecular descriptors; combining the characteristic molecule descriptors with the different chromatographic conditions into a complete data set; the complete data set serves as an input variable, retention time serves as an output variable, and a gradient elevator GB algorithm or a random forest method RF is adopted for modeling to obtain a QSRR model; and predicting the retention time of flavonoid or anthraquinone compounds under different chromatographic conditions by using the constructed QSRR model.

The invention belongs to the technical field of natural pharmaceutical chemistry, and discloses a flavonoid compound in lomatogonium radiatum as well as a preparation method and application thereof.The flavonoid compound is separated from lomatogonium radiatum of a gentiaceae lomatogonium plant, the medicinal material of lomatogonium radiatum is extracted and concentrated, and the flavonoid compound is obtained. And sequentially carrying out macroporous adsorption resin separation, gel column chromatography separation, medium-low pressure ODS column chromatography separation and semi-preparative high performance liquid chromatography separation. Pharmacological studies show that the compound provided by the invention can effectively inhibit the levels of COX2, IL-6 and TNF-alpha in mouse macrophages RAW246.7 caused by lipopolysaccharide (LPS), which indicates that the compound has obvious anti-inflammatory activity and can be used for preparing anti-inflammatory drugs.

The invention relates to a flavonoid derivative containing a flavonoid that is coupled to an alkylene group via an ether bond, said alkylene group having an adhesion group for adhesion onto a dental surface. The flavonoid derivative has a long-term suppressing effect on the dental disease-causing formation of biofilm on the dental surfaces treated with the flavonoid derivative. The invention further relates to a kit for producing such a flavonoid derivative, and to the use of same.

The application discloses a three-channel fluorescence sensor array based on sulfur quantum dots and a preparation method and detection application thereof. The sensor array is composed of three kinds of sulfur quantum dots, namely C-SQDs, PSS-SQDs and beta-SQDs. When target objects exist, the three kinds of sensing units generate differentiated fluorescence responses, forming a unique "fingerprint map". By using principal component analysis to analyze the response mode, the array can successfully distinguish between structurally similar baicalein, fiscetin, myricetin, quercetin and rutin in a concentration range of 8.0-56 muM. In addition, the array can also perform semi-quantitative analysis on a single flavone compound in a range of 4.0-56 muM, and the first principal component has a good linear relationship with the logarithm of the concentration of the target object.

This invention belongs to the field of traditional Chinese medicine treatment, specifically relating to an anti-cold and detoxifying capsule pharmaceutical composition, its preparation method, and its application. The composition comprises the following components in parts by weight: 25-35 parts Angelica dahurica, 40-50 parts Scutellaria baicalensis, 2-5 parts flavonoids, 0.5-2 parts Imperatorin, 1-5 parts Artemisia capillaris, 1-5 parts phloroglucinol compounds, and 0.1-0.5 parts organic acids. The component-based composition provided by this invention has a good effect in preventing and treating wind-heat colds, and the components exhibit good synergistic effects.

A method for detecting natural flavonoid selenium, including the following steps: S1: preparing mobile phase; S2: preparing test solution; S3: testing the test solution, the present disclosure is applicable to the field of pharmaceutical technology and provides the detection method for natural flavonoid selenium to ensure direct quantification of a quality control in drug development and application. Through a standard curve, a compensation effect of a dual ternary pump can be utilized to render a response value of each substance passing through a detector correspond to an amount of substance, without being affected by the fluidity gradient and without the need to prepare standard samples again.

The invention relates to the technical field of functional food, in particular to a tartary buckwheat and roxburgh rose composition for regulating blood pressure and blood fat and a preparation method thereof. The tartary buckwheat and roxburgh rose composition comprises tartary buckwheat and roxburgh rose. The mass ratio of the tartary buckwheat to the roxburgh rose is 1: (1-3). In-vitro activity evaluation, cell experiments and animal experiments prove that flavonoid compounds of tartary buckwheat and active ingredients such as vitamin C of roxburgh rose in the composition generate a synergistic effect, so that the antioxidant capacity, the ACE inhibition rate, the cholesterol clearance rate and the pancreatic lipase inhibition rate can be remarkably improved, NO generation is effectively promoted, and the curative effect is good. The contents of TC, TG and LDL-L are reduced, the HDL-L level is increased, and the balance of vascular active substances is regulated. The raw materials are all medicinal and edible, are high in safety and free of side effects, provide a natural and efficient auxiliary conditioning scheme for chronic metabolic diseases such as hypertension and hyperlipidemia, and have a good industrialization prospect.

This invention discloses an isoflavone compound, its preparation method, and its applications. The compounds are derived by directional esterification modification of all phenolic hydroxyl groups with allyloxycarbonyl chloride, o-chlorobenzoyl chloride, or cinnamoyl chloride, using 4',7-dihydroxyisoflavone or 4',5,7-trihydroxyisoflavone as the parent nucleus. The invention also provides a mild and efficient preparation method for these compounds and confirms their significant anti-inflammatory pharmacological activity. Pharmacological studies show that compounds B and E, in particular, exhibit excellent in vitro and in vivo anti-inflammatory activity, with compound E showing superior activity compared to the positive control drug dexamethasone. These compounds can be used to prepare drugs for treating inflammatory diseases such as pneumonia and colitis.

The inventors have found that quercetin and genistein, individually, suppress the expression of some aging-related genes while promoting the expression of other aging-related genes. Therefore, the main objective was to maintain the repressive effect on some aging-related genes that quercetin, genistein, and their glycosides may have, while suppressing the effect of promoting the expression of other aging-related genes. [Solution] The above problem can be solved by combining two or three components selected from the group consisting of component (i): quercetin and / or its glycosides, component (ii): genistein and / or its glycosides, and component (iii): nobiletin and / or its glycosides.

Disclosed are compositions and / or methods of use of the compositions for patients with neuronal diseases such as AD, Parkinson's, Huntington's, multiple sclerosis, and ALS. In certain embodiments flavonoids alone, or in a pharmaceutical preparation, are administered through the nasal olfactory route. In certain embodiments the flavonoid is apigenin and the neural disease is Alzheimer's. In some embodiments a porosome complex is administered for reconstitution into a neural cell. In certain embodiments, a co-administered blood-brain barrier traversing peptide is configured as a mimic of a domain of ATP 1 A3 and / or Tubulin.

The invention relates to a process for producing microparticles loaded with at least one flavonoid and / or flavonoid derivative, comprising the following successive steps: a) Mixing a water component in the form of an aqueous solution comprising the at least one flavonoid and / or flavonoid derivative with an oil component to form a solution of polylactide-co-glycolide in an organic solvent, to form a water-oil emulsion; b) Mixing the water-oil emulsion from step a) with another water component in the form of an aqueous solution comprising at least one stabilizer, to form a water-oil-water emulsion, c) Mixing the water-oil-water emulsion with an aqueous salt solution; d) Removal of the organic solvent in the water-oil-water emulsion to form a suspension comprising the microparticles loaded with at least the flavonoid and / or flavonoid derivative; e) Separating the loaded microparticles from the liquid of the suspension. Furthermore, the invention relates to microparticles, the use of which for the manufacture of a medicinal product, a dispersion containing the microparticles for use in the treatment and / or prophylaxis of oral infections, as well as non-medical use in cosmetics.

The application discloses a kind of extraction equipment and method for high-purity rutin, it is related to high-purity rutin extraction technical field, including equipment seat, equipment seat is provided with chromatography extraction mechanism, chromatography extraction mechanism is sequentially arranged from top to bottom by first chromatography column, second chromatography column and third chromatography column, first chromatography column and second chromatography column are connected by first connecting component, second chromatography column and third chromatography column are connected by second connecting component, the chromatography column of three different fillings is used in the application, macroporous adsorption resin filling preliminary enrichment rutin and remove macromolecular impurities, polyamide filling further separates rutin and other flavonoids, silica gel filling is fine purification, the extraction purity of rutin is effectively improved in multi-stage separation process, the flow guide vane of first connecting component and external rotary drive component can accurately adjust solution flow rate and flow direction, and optimize elution effect.

The invention provides application of nitraria tangutorum bobr extract in preparation of products for regulating glycolipid metabolism disorder, and relates to the technical field of plant extract application. The nitraria macrocarpa extract is a composition rich in alkaloid and flavonoid compounds, wherein the sum of the content of total alkaloid and the content of total flavonoids is not lower than 85% of the total weight of the extract. The extract is prepared through the processes of fresh fruit juicing, alcohol precipitation impurity removal and AB-8 macroporous resin gradient elution. Experiments show that the extract can significantly reduce fasting blood glucose of type 2 diabetes mice induced by high-fat and high-sugar diet combined with STZ, improve sugar tolerance and insulin resistance, and synchronously regulate the blood fat level, and shows an excellent glucose and lipid metabolism synergistic improvement effect. The extract disclosed by the invention provides a choice for developing a safe and effective glycolipid metabolism regulator.

The invention belongs to the technical field of biological medicines, and particularly relates to a flavone-copper complex for promoting healing of diabetic foot ulcer, an external preparation of the flavone-copper complex, and a preparation method and application of the flavone-copper complex. According to the flavone-copper complex disclosed by the invention, a flavone compound and copper ions form the complex, so that the healing of the diabetic foot can be effectively promoted; meanwhile, after the flavonoid compound and the copper ions are compounded, slow release of the copper ions and the flavonoid components can be achieved, the medicine effect is improved, meanwhile, the potential toxicity of the copper ions is reduced, and the flavonoid compound can be directly used as a medicine for relieving ulcer of the diabetic foot.

The application discloses application of xanthohumol in preparation of a medicine for resisting Aeromonas hydrophila infection of aquatic animals, and the xanthohumol can inhibit the activity of aerolysin of the Aeromonas hydrophila at a sub-inhibitory concentration, has a significant inhibitory effect on cell toxicity mediated by the aerolysin of the Aeromonas hydrophila, and further constructs an animal model infected by the Aeromonas hydrophila, and finds that the treatment of the xanthohumol can significantly improve the survival rate of the infected animals. The xanthohumol is one of main components of Chinese medicine hop, belongs to isoprenyl flavonoids, has biological functions such as bacteriostasis, anticancer, antioxidation, antiviral and hypoglycemic, and has high safety, and is an ideal medicine raw material or feed additive for preventing and treating the Aeromonas hydrophila infection of the aquatic animals.

The present invention relates to a use of a moiety selected from the group consisting of: (a) a nucleic acid encoding shRNA or siRNA specific for NR4A1 comprising the sequence of SEQ ID NO: 5 or SEQ ID NO: 6; (b) a NR4A1 antagonist selected from the group consisting of 1,1- bis(3'-indolyl)-1-(p-hydroxyphenyl)methane (DIM-C-pPhOH), resveratrol, a 3-chloro-5- methoxy analog (DIM-C-pPhOH-3-Cl-5-OCH3) and a flavonoid selected from the group consisting of kaempferol and quercetin; (c) a vector comprising a nucleic acid molecule encoding menin; and (d) a NF-ĸB inhibitor, in the manufacture of a medicament for treating pancreatic neuroendocrine tumors (PNETs) in a subject, wherein the medicament is to be administered to the subject, thereby reducing cellular proliferation of PNETs. The present invention also relates to a method of treating pancreatic neuroendocrine tumors (PNETs) in a subject.

Provided is a membrane permeation promoter that promotes permeation of a conjugate of a target substance and a transmembrane peptide into a lipid bilayer membrane, the membrane permeation promoter containing a flavonoid. Also provided are a membrane permeation promoting method and intracellular delivery method using the membrane permeation promoter, and an intracellular delivery kit comprising the membrane permeation promoter.

The invention relates to the technical field of natural active ingredient extraction, and particularly discloses a method for extracting flavonoid compounds from sophora alopecuroide matrine production waste liquid, and the method comprises the following steps: sequentially adding a reducing agent and a chelating agent into alkaline water waste liquid, uniformly stirring, standing, and filtering to obtain pretreated filtrate; dropwise adding organic acid into the pretreated filtrate to adjust the pH to obtain a suspension; standing and curing the suspension, centrifugally collecting precipitate, washing, and freeze-drying to obtain flavone powder. According to the method, the reducing agent and the chelating agent are combined for use, the precipitation efficiency and purity of the flavonoid compounds are remarkably improved and the impurity content is reduced through the dual effects of chemical reduction and physical adsorption, the yield of the flavonoid compounds is improved through the synergistic reaction of the reducing agent and the chelating agent, and the dosage of chemicals and the complexity of waste liquid treatment in the production process are effectively reduced; and energy conservation, environmental protection and economic benefits are improved.

The invention discloses an antioxidant additive for plateau injury protection as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides an antioxidant additive for plateau injury protection, the antioxidant additive is composed of a natural flavonoid compound and pharmaceutically acceptable auxiliary materials, the natural flavonoid compound is hispidulin, and the number of the pharmaceutically acceptable auxiliary materials is one or more. The antioxidant additive provided by the invention is based on traditional Chinese medicine active ingredients, has the efficacy of resisting plateau injury, and has a remarkable relieving effect on oxidative stress and inflammatory response syndromes caused by plateau injury; the antioxidant additive is simple in composition and strong in antioxidant effect, and can effectively inhibit release of inflammatory factors of a body, reduce organ injury, reduce inflammatory cascade reaction and play a role in resisting plateau injury.

The application discloses application of a natural flavonoid compound of a plant source in preparation of a medicine for inhibiting conjugative transfer of a drug-resistant plasmid, and drug-resistant plasmid conjugative transfer tests show that a plurality of flavonoid compounds can significantly inhibit conjugative transfer of a multidrug-resistant RP4-7 plasmid and a plurality of clinical plasmids carrying different drug-resistant genes, and the inhibiting ability on mcr-1 positive plasmid is more significant. The natural flavonoid compound provided by the application can simultaneously inhibit conjugative transfer of a drug-resistant plasmid in vitro and in vivo, and provides a new idea for blocking the spread and diffusion of drug resistance.

The inventors' primary objective was to provide a technology that suppresses inflammation, particularly in aging tissues. [Solution] The above problem can be solved by a composition containing at least one selected from the group consisting of genistein, quercetin, fisetin, nobiletin, kaempferol, apigenin, and naringenin, and glycosides thereof.

The invention discloses extraction equipment and method for high-purity rutin, and relates to the technical field of high-purity rutin extraction.The extraction equipment comprises an equipment base, a chromatographic extraction mechanism is arranged on the equipment base, and the chromatographic extraction mechanism is composed of a first chromatographic column, a second chromatographic column and a third chromatographic column which are sequentially arranged from top to bottom; the first chromatographic column and the second chromatographic column are connected through a first connecting assembly, the second chromatographic column and the third chromatographic column are connected through a second connecting assembly, the three layers of chromatographic columns with different fillers are adopted, and the macroporous adsorption resin filler is used for preliminarily enriching rutin and removing macromolecular impurities; rutin and other flavonoid compounds are further separated through polyamide filler, fine purification is conducted through silica gel filler, the extraction purity of rutin is effectively improved through the multi-stage separation process, the flow speed and the flow direction of a solution can be accurately adjusted through guide blades of a first connecting assembly and an external rotation driving assembly, and the elution effect is optimized.

The invention relates to a quince seed antioxidant extract as well as a preparation method and application thereof. The preparation method of the quince seed antioxidant extract comprises the following steps: crushing dried quince seeds to prepare quince seed powder; soaking the quince seed powder in a first solvent to prepare a crude extracting solution; the first solvent comprises ethanol and water; concentrating and drying the crude extracting solution to obtain a crude extract; mixing the crude extract with water to obtain an extract aqueous solution; mixing the extract aqueous solution with petroleum ether, and carrying out first extraction treatment to obtain a petroleum ether extraction part and a petroleum ether extracted aqueous solution; mixing the petroleum ether extracted aqueous solution with ethyl acetate, and carrying out second extraction treatment to obtain an ethyl acetate extracted part and an ethyl acetate extracted aqueous solution; the ethyl acetate extraction part is subjected to first concentration treatment, and a first quince seed antioxidant extract is prepared. According to the method, the flavonoid compounds can be efficiently and specifically enriched from the quince seeds.

The invention relates to a method for extracting total flavonoids of litsea coreana, which comprises the following steps: step 1, pretreatment: crushing and sieving dried litsea coreana leaves to obtain litsea coreana powder; step 2, enzymolysis: adding the litsea coreana powder into a buffer solution, then adding a compound enzyme, and carrying out enzymolysis treatment; step 3, DES extraction: adding a deep eutectic solvent into the material subjected to enzymolysis for extraction, and centrifuging to obtain an extracting solution; and step 4, purification: adding an anti-solvent into the extracting solution to precipitate the flavonoid compound, centrifuging again, collecting the precipitate, washing with a detergent, and drying to obtain the total flavonoid extract of the litsea coreana. According to the method, organic solvents and high-temperature conditions are not used, under the condition that the temperature is controlled to be 60 DEG C or below in the whole process, the extraction rate of the litsea coreana is greatly increased, the shelf life of the litsea coreana flavonoid extract is prolonged, and the method is simple, easy to operate and convenient to apply and popularize and has good industrial prospects.

An antifungal compound / conjugate is derived from quercetin, a naturally occurring flavonoid, through a strategic conjugation approach. The resulting quercetin conjugate exhibits enhanced antifungal activity and improved pharmacological properties compared to its parent molecule. The conjugate demonstrates potent efficacy against a range of drug-resistant fungal pathogens, including Candida auris, a critical threat in clinical settings due to its multidrug resistance. The invention also encompasses methods for synthesizing the conjugate, formulating the conjugate in a pharmaceutical preparation, and methods of use for treatment and prevention of superficial and systemic fungal infections.

The utility model relates to the technical field of sanitary napkins, in particular to a herba portulacae bacteriostasis and odor removal chip which comprises a herba portulacae bacteriostasis chip body, and a plurality of reaction through holes which are evenly formed and used for increasing the release area of flavonoid compounds are formed in the upper end face of the herba portulacae bacteriostasis chip body in a penetrating mode. The purslane antibacterial chip has the advantages that odor removal and bacteriostasis can be realized by the aid of the odor removal blocks and the purslane antibacterial chip, air can be isolated from the odor removal blocks and the purslane antibacterial chip by the aid of the instant isolating membrane, and the odor removal blocks and the purslane antibacterial chip can be used for removing odor and bacteria by the aid of the instant isolating membrane. According to the preparation method, flavonoid compounds in the herba portulacae antibacterial chip can be effectively prevented from being released in the storage process and playing a role in the early stage of use, so that the antibacterial or bactericidal effect is reduced in the later stage when bacteria are easier to breed, and the stability and effective bacteriostasis of the herba portulacae antibacterial chip in the long-time use process are ensured.

The invention belongs to the field of natural pharmaceutical chemistry and the field of cosmetics, and particularly relates to a skin care composition, a skin care product and application thereof.The skin care composition comprises silymarin, epigallocatechin gallate and quercetin, and the mass ratio of the silymarin to the epigallocatechin gallate to the quercetin is (3-7): (3-10): (1-5). The skin care product comprises the skin care composition, the content of the skin care composition is 0.5-10%, the skin care composition or the skin care product is applied to preparation of the skin care cosmetics, the esterified lecithin is used for encapsulating the silymarin, the epigallocatechin gallate and the quercetin, and sodium hyaluronate is matched, so that the skin care composition or the skin care product can be used for preparing the skin care cosmetics. The problems that flavonoid compounds are difficult to dissolve, permeate and absorb are effectively solved, and meanwhile, the skin care composition and sodium hyaluronate have a synergistic effect, so that the moisturizing, wrinkle removing and aging resisting effects are remarkably improved.

The application discloses a method for catalyzing sodium borohydride to produce hydrogen at low temperature, which is characterized by using natural flavonoids (including flavone, flavonol, isoflavone and the like) as a catalyst, using methanol or a mixture of methanol and water as a reaction solvent, using NaBH4 as a raw material for hydrogen production, and performing alcoholysis of NaBH4 to produce hydrogen. The catalyst used in the application is abundant in source and low in price, can catalyze sodium borohydride to produce hydrogen through alcoholysis at a wide reaction temperature range, has stable catalytic performance, high hydrogen production rate, good catalytic durability and reusability, and has important significance for the application and popularization of sodium borohydride in the field of hydrogen production.