314 results about "Genistein" patented technology

The disclosed methods and compositions for reducing cardiovascular risk factors in mammals generally includes using genistein to modulate various inflammatory and cardiovascular risk markers including: homocysteine, C-reactive protein, fibrinogen, sex hormone-binding globulin, fasting glucose, insulin, insulin resistance, and osteoprotegerin.

The invention provides an anti-stress fermentation protein feed which is produced by composite bacterium inoculation and high-temperature and moderate-temperature fermentation of a plant derived protein raw material, a terrestrial animal derived protein raw material and a marine animal derived protein raw material. Macromolecular substances in the protein feed are digested and decomposed into micromolecular substances, stressors in nutritional ingredients are decreased, anti-stress active factors such as genistein, glutathione and glucosamine are increased, the immunity of fed animals is improved, and the protein feed shows a good anti-stress capability. The invention further provides a producing method of the anti-stress fermentation protein feed. The feed product with high functionality, good anti-stress effect and high economical efficiency is obtained with the adoption of the multiple derived protein raw materials, a specific formula and an appropriate matching ratio and by mixed fermentation through a special secondary fermentation process.

The invention relates to a new method for promoting the fruit coloring, private for fruit coloring. Spraying 10-40mg / l cytisoside containing 0.1% broad adhesive on the fruit surface, and one time is ok before the fruit matures, or immersing the fruit with 10-40mg / l cytisoside containing 0.1% broad adhesive for 3-5 s after harvesting the fruit, drying in the dark and keep for 12 h, then exposing the fruit to the artificial light source or sunlight for 3-5 days for coloring. The method can be used for coloring fruit such as apple, pear, peach, plum, apricot, grape and strawberry which cultivated with bag covered or without bag covered. The method is simple, high effective, fast and safe, improves the fruit coloring degree and apparent quality.

The present disclosure provides for a scientific formulation useful in the treatment and prevention of human and animal diseases. A biologically effective amount of each of the components of the formulation is administered to patients in pill (or capsule) form via multiple different and identifiable pills. The compounds of the formulation are segregated into different pill types, and contain various amounts of the compounds Curcumin, Genistein, Squalamine, Vitamin E, N-Acetyl-Cysteine, Methylselenocysteine, Zinc Gluconate, B Complex, Lentinen, Coenzyme Q10 Acetyl-L-Carnitine, Lipoic Acid, Resveratrol, and Vitamin C. Furthermore, Arabinoxylan and / or Peperine may be added to the various pill formulations.

The invention relates to a method for extracting, separating and purifying an active component of Chinese herbal medicine, namely genistein from roots and stems of pigeon pea, and aims at providing a method for easily, safely, economically and effectively extracting, separating and purifying the high-purity genistein from roots and stems of the pigeon pea. The adopted technical proposal comprises that: fresh or dry roots and stems of the pigeon pea are used as raw materials, and by adopting the homogenate induction technique, the enzyme and acid induction technique, the solvent extraction technique, the ultrasonic oscillation flocculation technique, the negative pressure cavitation suspension solid extraction technique, the macroporous absorbent resin enriching technique, the positive phase silica gel medium-pressure column chromatography separation technique and the low-temperature crystallization and re-crystallization technique, the refined genistein is obtained, and the purity of the genistein reaches more than 95 percent. The method has the advantages of abundant and easily obtained raw materials, simple and convenient process, high yield and purity of the obtained genistein, suitability for industrialized production and high added value of products.

New compositions and methods for treating patients suffering from Amyotrophic Lateral Sclerosis (ALS) and other metabolic and autoimmune disorders, which include glycopeptides such as N-acetyl-D-glucosaminyl(β1-4)-N-Acetyl-muramyl-L-alanyl-D-isoglutamine (GMDP) and peptide analog-L-alanyl-D-glutamic acid (GMDP-A) of at least 98% purity administered either alone, or in combination with a flavone such as luteolin and / or an isoflavone such as genistein, optionally in combination with a flavonol glycoside such as isoquercitrin or rutin. The high purity glycopeptides have a decreased amount immunogenic impurities and demonstrate a synergistic effect when combined with luteolin and / or genistein in presence of isoquercitrin.

A formulation of supplement is able to boost the natural cells, both increasing the population of the natural cells and enhancing the natural cell's activity. The formulation can be administered via different forms, such as tablet, injection, skin absorption and etc. This formulation comprises 16 different components, including Alpha Lipoic Acid, Arabinoxylin, Curcumin, Garlic, Genistein, Ginseng, Lentinan, Mistletoe, N-Acetylcysteine, Resveratrol, Selenium, Vitamin B Complex, Vitamin C, Vitamin D3, Vitamin E and Zinc. By virtue of enhancing the natural killer cells (NKC) in the body, the formation is helpful for the prevention and treatment of a number of different human or animal diseases, such as cancers and viral infections. In addition, certain synergistic effects have been observed between different components in the formulation, which further enhances the beneficial effect of the formulation.

The present invention provides a new type balloon dilation catheter which includes ballon and medication material coated on stent. Said medication material comes from one or two and more than two mixtures of heparin sodium, fiber degrading enzyme, serine proteinase, batroxobin, aspirin, genistein, hirudin and its recombined product, colchicine, sirolimus, biolimus, zotarolimus, tracrolimus, pimecrolimus, simvastatin, atorvastatin, pravastatin, ciclosporin, Anti-CD34, dexamethasone, bleomycin, plicamycin, daunomycin, mitomycin C, actinomycin D, taxol, celastrol, methopterin, 5-fluorouracil, cytarabine and 6-purinethol. The balloon is made of macromolecule nylon material, and the stimulation to blood vessel is far lower than the stent with metal structure.

The invention provides a method for screening a xanthine oxidase inhibitor by ultra performance liquid chromatography and mass spectrometry. The method is used for analyzing the in-vitro inhibition rate of the extract or monomer of a natural product on the xanthine oxidase and the catalytic activity of the xanthine oxidase. By using the ultra performance liquid chromatography-mass spectrometry in the xanthine oxidase inhibitor screening, the method is rapid and accurate in sample detection, and the correlation coefficients of the linear equation reaches 0.998. The mass spectrometry has high accuracy and good specificity in detecting the mass electron ratio of a compound, and can be used for screening the xanthine oxidase inhibitor and for the kinetic study of the xanthine oxidase inhibitor without false positive and false negative results which occur in the spectrometry. The results showed that the inhibition rate I of 20 mumol / L allopurinol is 80%; the inhibition rate of 20 mumol / L isorhamnetin is 73%; the inhibition rate of 20 mumol / L genistein is 50%; and the inhibition rate of 0.1 mg / mL aqueous extract of Ginkgo biloba is 27%.

Agents that stimulate nuclear translocation of Nrf2 protein and the subsequent increases in gene products that detoxify and eliminate cytotoxic metabolites are provided in a method for treating diabetic retinopathy or drusen formation in age-related macular degeneration. The structurally diverse agents that act on the Nrf2 / ARE pathway induce the expression of enzymes and proteins that possess chemically versatile cytoprotective properties and are a defense against toxic metabolites and xenobiotics. Agents include certain electrophiles and oxidants such as a Michael Addition acceptor, diphenol, thiocarbamate, quinone, 1,2-dithiole-3-thione, butylated hydroxyanisole, flavonoid other than genistein, an isothiocyanate, 3,5-di-tert-butyl-4-hydroxytoluene, ethoxyquin, a coumarin, combinations thereof, or a pharmacologically active derivative or analog thereof.

The present invention provides compositions and methods for the prophylactic and therapeutic treatment of animals, including humans from radiation injury. In particular, the present invention provides methods and compositions comprising the isoflavone genistein (4′,5,7-trihydroxyflavone) or phytoestrogenic isoflavonoids.

The invention relates to a breeding method for backup boars and sows. The method includes the following steps of giving following feed including 400-600 parts of pennisetum hydridum, 600-700 parts of maize, 50-100 parts of wheat bran, 200-300 parts of bean pulp, 20-50 parts of fish meal, 0.1-0.3 part of vitamin, 1-2 parts of kudzuvine root extract, 1-2 parts of rhizome drynariae extract, 1-2 parts of morinda officinalis extract, 0.1-0.2 part of genistein, 1-4 parts of fructose and 1-5 parts of glucose; making backup boars and sows freely eat the feed; regularly giving extract each year for a week each time, wherein the extract is prepared from, by weight, 5-20 parts of poria cocos, 5-10 parts of astragalus membranaceus, 1-3 parts of fennel and 1-5 parts of corydalis saxicola bunting. By means of the method, health of backup boars and sows can be effectively improved, the estrus rate is increased, and the breeding success rate of backup boars and sows is increased.

The method for preparing soybean isoflavone aglycone by using enzyme method to hydrolyze soybean isoflavone glycoside glycosyl includes the following steps: using the enzyme capable of hydrolyzing soybean isoflavone glycoside glycosyl, mixing said enzyme, soybean isoflavone glycoside and buffer solution to make reaction for 1-40 hr., its reaction temp. is 4-75 deg.C and pH value is 3-9, so as to extract soybean isoflavone aglycone. Said invention not only can use enzyme to hydrolyze monoglycoside-soybean glycoside and genistein glycoside and convert them into correspondent aglycone, but also can use enzyme to hydrolyze soybean glycoside and genistein glycoside mixed glycoside and convent them into the mixed aglycone. Said product can be used as raw material for making medicine and health-care product.

The present invention discloses a swine gender-controlling sperm and preparation method and application thereof, which comprises diluting the original boar semen with the original semen diluent containing seminal plasma, genistein and cow urine phenol, separating the diluted original boar semen by the flow cytometry and recycling the obtained swine gender-controlling sperm into the freezing diluent containing seminal plasma, genistein and cow urine phenol. The method provided by the present invention has simple operation, which can effectively prevent the separation and the cryopreservation of pig X, Y sperm from damaging, thereby improving the sperm quality and the fertilization ability, and prolonging the survival time of sperm, wherein the separated sperm can be used in low dose insemination, so as to reduce the production cost. The method can be widely applied to commercial promotion with broad market prospects.

The invention discloses an AUH-JLC159, whose preservation number is CGMCC No. 5304, and the usage thereof in preparation of (-)-5-OH-equol. The invention comprises the following steps: (1) cultivating the bacterial strain AUH-JLC159; (2) carrying out co-culture of genistein and bacterial strain AUH-JLC159 and separation and purification of the metabolite: switching the seed liquid of bacterial strain AUH-JLC159 to the BHI medium, and cultivating for 2-3 days in an anaerobic working station, thereby the substrate genistein is transformed into (-)-5-OH-equol. Purifying and separating the metabolite. The AUH-JLC159 provided by the invention can transform the substrate genistein to 5-OH-EQ with high efficiency under the anaerobic condition; thereby solving the problem of microbe biosynthesis and resource scarcity of 5-OH-EQ, and has a great promotion effects for physiology and pharmacological activities of 5-OH-EQ and new drug research and development.

The present invention relates to a composition for slimming, more particularly, to a slimming composition containing theanine and at least on selected from the group consisting of caffeine, genistein, L-carnitine and catechin. The composition of the present invention contains theanine and each of caffeine, genistein, L-carnitine or mixtures thereof, and has properties of decomposing fats, hydrolyzing lipid and removing cellulites.

Belonging to the technical field of cell mediums, the invention in particular relates to a serum-free medium for rapid generation of induced pluripotent stem cells. The serum-free medium for rapid generation of induced pluripotent stem cells includes a basic medium and an additive, and the additive comprises the following components in terms of final concentration: 20-40ng / mL cell growth factor, 21-30microg / mL transferrin, 6-9microg / mL fibronectin, 10-15microg / mL polyvinylpyrrolidone, 1-3microg / mL genistein, 4-7microg / mL mannitol, 5-8microg / mL insulin, 11-15microg / mL L-glutamine, 12-16microg / mL palmitic acid, and 8-15microg / mL hesperidin. The serum-free medium for rapid generation of induced pluripotent stem cells provided by the invention has the advantages of clear components, no serum, high safety, and rapid generation of induced pluripotent stem cells.

The present invention relates to a composition containing genistein that accelerates the expression of the Carnitine Palmitoyl Transferase-1 (CPT-1) for improving or treating obesity. More particularly, the composition of the present invention comprises genistein that accelerates the expression of the CPT-1, an important enzyme for the lipolysis of fatty acid, and comprises carnitine that transports fatty acid into mitochondria and accelerates oxidation of fat in the oxidation process of fatty acid.

The invention discloses a medicine for treating cancer. The common rabdosia leaf cancer-control anticancer formula one is prepared by the following steps: mixing the raw materials of common rabdosia leaf, red ginseng, mulberry, medlar, astragalus root, bunge corydalis herb, honeysuckle and ligusticum chuanxiong hort according to a reasonable ratio, adding a proper amount of water, decocting, drying, grinding into fine powder, and then compounding with cordyceps powder, chitin powder, genistein powder which are ground into fine powder; aloe mineral composite anticancer formula two is prepared by the following steps: grinding aloe crystalline powder with pollen, royal jelly powder, fructooligosaccharide, magnesium lactate, zinc lactate, tricalcium phosphate, sodium selenite and chromium trichloride into fine powder according to proportion, and then mixing with vitamin A, B1, B2, B3, B6, B9, B12, E and K; sea-buckthorn nutritional polysaccharide anticancer formula three is prepared by the following steps: grinding sea-buckthorn powder, inulin and black tea powder into fine powder according to proportion, and then mixing with fructooligosaccharide, oligo-isomaltose, mannite, vitamin C and sucralose; and high fiber detoxification anti-cancer formula four is prepared by the following steps: mixing natural fiber with fructooligosaccharide, isomalt, strawberry powder, sea-buckthorn powder and protein hydrolysate. The medicine is not only suitable for treating various cancer patients at each stage, but also has good healing effect, short period of treatment, no toxic or side effect, simple administration method, rich medicine sources and low cost, and is efficient and safe.

The invention discloses a method for promoting coloring of red Chinese pears by utilizing chemical agents. The method includes immersing uncolored Chinese pears which reach a physiological maturity stage into a coloring accelerator solution for 1-5 minutes, taking out the Chinese pears and drying the Chinese pears in the air, and placing the Chinese pears in an environment with a temperature of 12-22 DEG C, 70-90% of relative humidity, 0.1-0.5 micromole*m<-2>*s<-1> of luminous intensity of ultraviolet-b radiation (UV-B), 250-550 micromoles*m<-2>*s<-1> of luminous intensity of white light for 5-10 days, wherein the coloring accelerator comprises one or more kinds of ethephon, genistein and methyl jasmonate. According to the method, anthocyanin accumulation of the read Chinese pears can be effectively induced, the exterior quality of the pears can be improved, and the method and the coloring effect are better compared with the prior art after the immersing process of the red Chinese pears into the chemical agents.

The invention relates to a genistein compound and its preparation method, the genistein hydrogel compound is composed of genistein and hydrogel. The preparation method comprises the following steps: taking hydrogel and adding in an aqueous medium, heating and stirring to fully dissolve to obtain a hydrogel solution; taking genistein and adding in an alkaline aqueous medium to obtain a genistein solution; mixing the obtained hydrogel solution and the obtained genistein solution and stirring, fully drying to obtain the genistein hydrogel compound. According to the invention, composition of a hydrophilic colloid carragheenan and genistein is used for raising the water-solubility of genistein. A single factor experiment is used for respectively inspecting the influence of composite temperature, the composite time, genistein concentration and endpoint pH on composite efficiency. A response surface methodology is used for optimizing the compound preparation process, the obtained technology is capable of producing the compound with the highest composite efficiency, and the water-solubility of genistein in the genistein hydrogel compound can be effectively enhanced.

The invention discloses a method for improving the water solubility of dye lignin by utilizing cyclodextrin glucosyltransferase transglycosylation reaction, and belongs to the field of enzyme engineering. A gene (GenBank accession no.JX412224), which is derived from P.macerans strain JFB05-01 (CCTCC NO:M208063) and optimized by a cyclodextrin glucosyltransferase codon, is connected to pET20b(+) plasmid and is transferred to an E.coli BL21(DE3) cell to establish gene engineering bacteria for producing the cyclodextrin glucosyltransferase. The pure cyclodextrin glucosyltransferase, which is obtained through fermentation enzyme production and purification, is applied to catalytic transglycosylation reaction, alpha-cyclodextrin serves as a glycosyl donor, the dye lignin serves as glycosyl receptor, and at least four dye lignin glycosylated derivatives (Glc)n-genistein (n is equal to 1, 2, 3 and 4) are finally obtained, wherein Glc-genistein and (Glc)2-genistein are main products, in particular the water solubility of the (Glc)2-genistein is greatly improved compared with that of the dye lignin.

A genistein sulfonates derivative and the preparation process are characterized in that the chemical general formula is showed, wherein R3 is toluene sulfonyl-oxygen, R1 is OR4, R2 is OR5, and R4 and R5 are hydrogen, methyl, ethyl group, benzene sulfonyl and acetyl.

A method for preparing genistein from fructus sophorae takes fructus sophorae as raw material and includes that: bio-enzyme with quantity of 1-2% of that of the raw material is added for heat-preservation enzymolysis for 10-15 hours at the temperature of 45-55 DEG C, the enzymolysis product is performed with ethanol backflow extraction for 2-3 times, and then decompression concentration and placement crystallization are carried out, hot water scrubbing crystallization is conducted and the backflow dissolving is performed subsequently, finally placement crystallization is carried out while the pH is 4-5. The invention prepares the product with high content, realizes high yield, has low cost, and is easy for scale-up and industrialization.

The invention discloses a cyclodextrin glucosyltransferase and a preparation method thereof, and belongs to the field of genetic engineering and enzyme engineering. The glycosylation efficiency of pigment is improved by 23%, 44% and 32% respectively by replacing alanine (Ala) at the No. 156 site of CGTase of P. Macerans strain JFB05-01(CCTCC NO: M208063) with lysine (Lys), glutamine (Gln) and valine (Val). Those mutant enzymes are more beneficial for catalyzing and producing glycosylated genistein compared with wild type CGTase.

The invention relates to a method for extracting, separating and purifying isoflavone active components biochanin A and genistein from dalbergia odorifera T.Chen leaves. The employed technical scheme comprises that biochanin A and genistein are respectively obtained both with the purity of 95% or more by taking dalbergia odorifera T.Chen leaves as raw materials and employing homogenate fragmentation enzyme induction technology, solvent extraction technology, cavitation oscillation dispersion technology, macroporous adsorption resin enrichment technology, normal-phase silica gel medium-pressure column chromatography separating technology, low-temperature crystallization and recrystallization technology, and the like. Because dalbergia odorifera T.Chen leaves are abundant in resource, the extraction process is simple, the separation cost is low and the method is time-saving and labor-saving, the method provided by the invention is applicable to large-scale production in industry, and has extremely high medical science value and wide market prospect.

The invention belongs to the field of chemical pharmaceutical technology, and in particular relates to application of a genistein derivative 4', 5'-dyhydroxy-7-[4-(N, N-diethylamino) butoxy] isoflavone in preparing a medicament for treating learning and memory disorder. 4', 5'-dyhydroxy-7-[4-(N, N-diethylamino) butoxy] isoflavone has the effect of promoting macrophage chemotactic fator (MCF)-7 cell proliferation and the action effect is in a bell jar type. The latent time of female castrated mice (oophorectomy is performed) in a water maze experiment is shortened and the error time of the mice is greatly decreased in a Y maze experiment. The fact indicates that 4', 5'-dyhydroxy-7-[4-(N, N-diethylamino) butoxy] isoflavone can effectively improve learning and memory capability. The improvement of learning and memory is consistent with the enhancement of phosphorylation of calcium-calmodulin dependent protein kinase (CaMk) II. 4', 5'-dyhydroxy-7-[4-(N, N-diethylamino) butoxy] isoflavone has good effects of improving learning and memory and can be used for preparing the medicament for improving learning and memory.

The invention relates to a chitosan oligosaccharide agricultural composition for improving coloration of plants and an application thereof and a method by utilizing the same to improve coloration of plants. The invention particularly provides a chitosan oligosaccharide agricultural composition for improving coloration of plants. The weight percentage of the chitosan oligosaccharide in the agricultural composition is 0.0015 to 0.015 percent and is preferentially 0.0025 percent to 0.01 percent. The chitosan oligosaccharide agricultural composition also comprises other ingredients such as one or multiple varieties of glycine, levulinic acid, glutamic acid, phenylalanine, tyrosine, acetylsalicylic acid, genistein, uniconazole, potassium sulphate, monobasic potassium phosphate, dipotassium phosphate, potassium phosphate, calcium chloride and boric acid. The plants include crops and flowers. An evaluation target of the plant coloration is a harvesting target or an ornamental target of the plant such as seeds, fruits, roots, stems, leaves and flowers.

The invention discloses a method for quickly determining six soy isoflavones in a bean product by utilizing UPLC (Ultra Performance Liquid Chromatography), and belongs to the technical field of analytical chemistry. The types of the tested bean product comprise soybean milk, bean curd, soy chicken, dried tofu, bean curds, dried beancurd sticks and the like, and the six soy isoflavones such as daidzin, isoflavoues Aglycone, Glycitin, glycitein, genistin and genistein are determined. The method comprises the following steps: firstly extracting the soy isoflavones in the bean product by using a methanol solution, after collecting a supernate fluid, repeatedly extracting residual twice by using methanol, merging the supernate fluids, and carrying out UPLC detection. Quick determination for the composition and content of the six soy isoflavones mainly in the bean product are achieved by adopting UPLC. The method is simple in sample pretreatment, high in analysis speed, high in repeatability and accuracy, and provides an efficient and accurate analytical method means for evaluating and controlling the quality of the bean product.