64 results about "Genistein" patented technology

Provided is a transfersome cosmetic composition including: a Matricaria flower extract, a Centella asiatica extract, an Arnica montana flower extract, a Scutellaria baicalensis extract, a Paeonia suffruticosa root extract, an aloe vera leaf extract, a Saccharina japonica extract, a licorice root extract, a lactobacillus lysate formed by fermenting strains belonging the lactobacillus genus, a rice bran fermentation filtrate formed by fermenting rice bran using a first composite strain including strains belonging to the bacillus genus, strains belonging to the lactobacillus genus, and yeast, a green tea fermentation extract formed by fermenting green tea using a second composite strain including strains belonging to the Aspergillus genus and strains belonging to the Lactobacillus genus, a grape seed oil, an jojoba seed oil, hydroxyacetophenone, Bifidobacterium, arginine, betaine, trehalose, sodium hyaluronate, hydrolyzed elastin, Ceramide NP, resveratrol, Genistein, tocopheryl acetate, glycerin, and purified water.

Disclosed is a method of treating a patient with Stargardt Disease. Also disclosed is a method of delaying the progression of vision loss in a patient with Stargardt Disease. The methods comprise administering to the patient from 10 mg to 35 mg per day of gildeuretinol acetate.

The invention discloses a method for increasing the content of genistin and genistein in flemingia philippinensis by using potassium. Culturing the flemingia philippinensis tissue culture seedlings in a propagation culture medium, and subculturing to obtain flemingia philippinensis stem segments with leaves; taking stems of the flemingia philippinensis, and culturing the stems in a rooting culture medium to obtain the flemingia philippinensis with the content of genistin and genistein increased; wherein the propagation culture medium is prepared from MS (Murashige and Skoog), 6-benzyladenine, indoleacetic acid and kinetin; the rooting culture medium comprises KNO3, MS (Murashige and Skoog) and naphthylacetic acid, and the MS does not contain KNO3. According to the method for increasing the content of genistin and genistein in the flemingia philippinensis by using potassium, accumulation of active ingredients of genistin and genistein at the root of the flemingia philippinensis is adjusted by adding exogenous potassium ions, so that the utilization rate of the flemingia philippinensis is increased, and the problem of resource shortage of the flemingia philippinensis is relieved.

The inventors have found that quercetin and genistein, individually, suppress the expression of some aging-related genes while promoting the expression of other aging-related genes. Therefore, the main objective was to maintain the repressive effect on some aging-related genes that quercetin, genistein, and their glycosides may have, while suppressing the effect of promoting the expression of other aging-related genes. [Solution] The above problem can be solved by combining two or three components selected from the group consisting of component (i): quercetin and / or its glycosides, component (ii): genistein and / or its glycosides, and component (iii): nobiletin and / or its glycosides.

The invention discloses a method for separating and extracting epicatechin, formononin and genistin from caulis spatholobi and application of the formononin, belongs to the technical field of natural product extraction and medical application, and relates to an efficient and controllable extraction process. According to the method, the three active ingredients of epicatechin, ononin and genitin can be enriched and purified at the same time from the caulis spatholobi, the potential medicinal value of ononin in gastric cancer treatment is further revealed, and a scientific basis is provided for deep development and comprehensive utilization of the caulis spatholobi.

The invention belongs to the field of biological medicine health research, and particularly relates to preparation and application of a gamma-cyclodextrin / genistein inclusion compound for relieving oxidative stress. The inclusion compound is formed by gamma-cyclodextrin and genistein through a self-assembly reaction in a solution. Compared with genistein, the inclusion compound can remarkably relieve oxidative stress injury of PC12 cells caused by organic hydrogen peroxide. Meanwhile, the gamma-cyclodextrin / genistein inclusion compound can remarkably improve the iron reducing capacity of genistein and the DPPH free radical scavenging rate. Therefore, the gamma-cyclodextrin / genistein inclusion compound has a good anti-oxidation effect and an effect of relieving oxidative stress. A new thought is provided for research of natural antioxidants, and a new view angle is provided for treatment of oxidative stress related diseases.

The invention relates to a recombinant microbial strain for de novo synthesis of gardenin B and application of the recombinant microbial strain in production of polymethoxylated flavone compounds, and belongs to a cross technology of synthetic biology and organic synthetic chemistry. The invention designs and constructs a heterozygous gardenin B biosynthesis pathway, and obtains a microbial strain for synthesizing gardenin B from the beginning. By screening F8H and redox partner types and regulating and controlling subcellular localization, an F8H-redox partner combination with optimal space and type is determined, and an engineering strain of which the gardenin B yield is greatly improved is obtained. The efficient synthesis method of the polymethoxylated flavonoids is established by selecting recombinant microorganisms for synthesizing gardenin B from de novo to perform co-culture fermentation or chemical conversion of fermentation products.

The invention relates to the technical field of food biology, in particular to a high-activity fermented astragalus mongholicus and red date compound for improving myocardial function, which is prepared by taking Xinjiang Qiang red dates and astragalus mongholicus as main raw materials and matching cellulase, pectinase, beta-glucanase and hydroxypropyl-beta-cyclodextrin. In the preparation process, gradient freezing-microwave pretreatment and ternary composite enzymolysis synergistic wall breaking are adopted, quaternary strains of bifidobacterium longum, bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum are compounded, adenosine concentration response type staged solid-state fermentation is performed, and microcapsule embedding stabilizing treatment is performed to obtain the product. The content of cAMP in the product is greater than or equal to 13.0 mu g / g, and the content of calycosin-7-glucoside in astragalus membranaceus is greater than or equal to 1.9 mg / g. The compound can be used for preparing myocardial ischemia pretreatment health-care food and a pharmaceutical composition for adjuvant therapy in the myocardial infarction recovery period, and the dosage forms comprise chewable tablets, oral liquid, capsules and the like.

The inventors' primary objective was to provide a technology that suppresses inflammation, particularly in aging tissues. [Solution] The above problem can be solved by a composition containing at least one selected from the group consisting of genistein, quercetin, fisetin, nobiletin, kaempferol, apigenin, and naringenin, and glycosides thereof.

The present invention relates to new solid forms (NSF) of a flavonoid and a coformer. The NSF are co-amorphous solids of flavonoids and amino acids, as well as the solvates of said co-amorphous solids. The flavonoid is selected from quercetin, genistein, fisetin, apigenin, naringenin, rutin, diosmin, among others. The coformer is selected from lysine and arginine. The NSF have enhanced stability, solubility and pharmacological properties over parent polyphenols. The new solid forms are suitable to be used in pharmaceutical compositions, food products, nutritional supplements and the like.

The application discloses genistein with CD38 inhibitory activity and a preparation method and application thereof. The preparation method comprises the following steps: crushing dried purslane, adding water and a composite enzyme into the obtained purslane powder to perform enzymolysis; adding ethanol into the obtained enzymolysis liquid to perform ultrasonic-assisted extraction, decompression concentration and organic solvent extraction; performing macroporous adsorption resin separation and elution on the obtained extraction liquid, collecting the eluate; performing gel column chromatography separation and liquid chromatography purification on the obtained eluate, and drying to obtain genistein. The preparation method is simple, the prepared genistein has high purity, and the highest purity can reach 99.05%; and the prepared genistein has good CD38 inhibitory activity, and opens up a new use of purslane genistein. + horizontal promotion of medicines, health products and food.

The invention discloses a high-yield beta-glucosidase strain and application of the high-yield beta-glucosidase strain in efficient conversion of genistin, and belongs to the technical field of microbial fermentation and enzyme engineering. In order to solve the problems of low enzyme activity and low conversion efficiency of a beta-glucosidase production strain in the prior art, the invention provides the lactobacillus mucus DQ7-1 of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36230, and the lactobacillus mucus DQ7-1 has the advantages that the lactobacillus mucus DQ7-1 has the preservation number of CGMCC No.36230; the strain is obtained through natural fermentation with soybean whey as a raw material in combination with aesculin color development and p-nitrobenzene-beta-D-glucopyranoside quantitative enzyme activity screening methods, and the beta-glucosaccharase activity of the strain is remarkably higher than that of common lactic acid bacteria. The strain is mainly used for catalyzing efficient conversion of soybean isoflavone glycoside, especially genistin, to genistein in the aglycone form, and has important application value in the fields of functional food, medicine and feed.

The invention provides application of dragon's blood and active ingredients thereof in preparation of a medicine for treating heart failure, and belongs to the technical field of biological medicine. It is found for the first time that the dragon's blood can significantly improve the heart function of a heart failure model animal, reduce the level of a heart failure marker and relieve cardiac hypertrophy, cardiac pulmonary edema, myocarditis infiltration and fibrosis; core active components such as genistein and 3-hydroxycinnamic acid are screened and verified, wherein the genistein can reproduce the overall treatment effect of the dragon's blood. The invention also discloses that the core action mechanism of the composition is to promote macrophage VEGFC secretion, increase heart lymphatic vessel density and promote lymphatic vessel neogenesis, and a brand new natural drug candidate scheme and an intervention strategy are provided for heart failure treatment.

The invention belongs to the technical field of biological medicines, and particularly relates to application of genistein to preparation of an anti-colorectal cancer medicine for inhibiting LINC00355 expression. According to the present invention, the genistein is adopted as the LINC00355 expression inhibitor so as to effectively inhibit the expression of the LINC00355, such that the adsorption effect of the LINC00355 on the miR-150 is relieved, the expression of the miR-150 is up-regulated, the expression of the SGK1 is further inhibited by the miR-150, the activation of the downstream EGFR / PI3K / Akt and MAPK signal channels is finally inhibited, and the activity of the downstream EGFR / PI3K / Akt and MAPK signal channels is inhibited; and inhibition of colorectal cancer cell proliferation, inhibition of colorectal cancer cell cycle G0 / G1 phase, induction of colorectal cancer cell apoptosis and inhibition of colorectal cancer cell migration and invasion ability are realized, so that a better anti-colorectal cancer treatment effect is achieved.

The invention discloses a method for preparing oligomeric genistein by oxidative conversion of genistein with laccase. According to the method, the coprinus cinerea laccase Lcc5 is used for preparing the oligomeric genistein, so that the condition for preparing the product is optimized, the conversion rate is increased to about 92%, and the product with higher polymerization degree is obtained. The antioxidant activity, the enzyme inhibition activity, the anti-amyloid aggregation activity and the antibacterial activity of the obtained conversion product are remarkably improved compared with those of monomer genistein. The enzyme has potential application value in oxidative conversion of genistein and other isoflavones to improve the biological value of genistein and other isoflavones.

The invention discloses an antibacterial polyurethane medical catheter material and a preparation method thereof, and relates to the technical field of polyurethane materials. When the antibacterial polyurethane medical catheter material is prepared, genistein and zinc acetate react to prepare a genistein complex; the preparation method comprises the following steps: sequentially treating boron nitride with sodium hydroxide and kh550 to prepare aminated boron nitride; reacting the aminated boron nitride with a phosphorus-containing flame retardant to prepare functionalized boron nitride; the preparation method comprises the following steps: reacting 2-amino-2-methyl-1, 3-propylene glycol with 4-pyridylaldehyde and 1-bromohexane in sequence to prepare a pyridinium salt monomer; the preparation method comprises the following steps: polymerizing polytetrahydrofuran and diphenylmethane diisocyanate, and carrying out chain extension on a pyridinium monomer to prepare polyurethane; the preparation method comprises the following steps: uniformly mixing polyurethane, a genistein complex and functionalized boron nitride to prepare an antibacterial polyurethane medical catheter material; the antibacterial polyurethane medical catheter material prepared by the preparation method disclosed by the invention has good antibacterial and flame-retardant effects.

There are described compositions comprising an effective amount of a combination of two or more components, said components selected from acacetin, ACTI peptide, alpha-lipolic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-beta-methyl-butyrate, Bacopa monnieri, catechin, catechol, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (−)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercitrin (EMIQ), kaempferol, kuromanin, leucine, lithium, luteolin, luteolin, luteolinidin, melatonin, menadione, 1-methylnicotinamide (MNA), methyl salicylate, myricetin, nadide, niacin (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley (Petroselinium crispum), phenylephrine, pokeweed mitogen, 15-Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxrutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin and zinc (including salts thereof).

The invention relates to the technical field of pharmaceutical preparations, and discloses a production process for treating body nodules, which comprises the following steps: S1, extracting and purifying active pharmaceutical ingredients, and preparing liposome quercetin, genistein and sodium hyaluronate; s2, preparing a functional carrier material, namely preparing polyunsaturated phosphatidylcholine and phosphatidylcholine nanoparticles by an emulsification-solvent evaporation method; the invention also discloses a formula for treating body nodules, and the formula comprises the following components in percentage by weight: 15-25% of active pharmaceutical ingredients; 65%-80% of a functional carrier material; 0.2%-1% of an auxiliary reinforcing material; and 5%-10% of a slow release regulator. Precise delivery and controlled release are realized through a multi-responsive nano-carrier, a multi-mode treatment system combining a photothermal effect and magnetic targeting is adopted, the drug targeting and the treatment efficiency are remarkably improved, the drug effect time is prolonged through a slow-release regulator, the drug use frequency is reduced, and the patient compliance is improved.

This invention discloses an analytical method for the application of wolfberry in the preparation of foods and drugs for rheumatoid arthritis. First, 36 active ingredients and 201 drug targets of wolfberry were obtained through a traditional Chinese medicine systems pharmacology database and analysis platform. Then, 536 targets for rheumatoid arthritis were predicted using Gene Cards and TTD databases, and 55 intersection targets were obtained through Venn diagram analysis. An intersection target network diagram and a "drug-ingredient-target-disease" network diagram were constructed. Topological analysis screened out four core targets: IL1B, JUN, IL6, and TNF, and five core ingredients. Molecular docking results showed that quercetin, genistein, β-sitosterol, stigmasterol, and lycopene strongly bound to the four core targets. This invention provides a theoretical reference for the development of drugs for the treatment of rheumatoid arthritis.

The invention discloses a method for increasing the content of genistein in flemingia philippinensis by using nitrogen. Culturing the flemingia philippinensis tissue culture seedlings in a propagation culture medium, and subculturing to obtain flemingia philippinensis stem segments with leaves; taking stems of the flemingia philippinensis, and culturing the stems in a rooting culture medium to obtain the flemingia philippinensis with increased genistein content; wherein the propagation culture medium is prepared from MS (Murashige and Skoog), 6-benzyladenine, indoleacetic acid and kinetin; the rooting medium comprises NH4NO3, MS (Murashige and Skoog) and naphthylacetic acid, and the MS does not contain NH4NO3. According to the method for increasing the content of genistein in the flemingia philippinensis by using nitrogen, accumulation of the active ingredient genistein at the root of the flemingia philippinensis is adjusted by adding exogenous nitrogen ions, so that the utilization rate of the flemingia philippinensis is increased, and the problem of resource shortage of the flemingia philippinensis is relieved.

The invention belongs to the technical field of medicines and disease treatment, and particularly relates to application of genistein to preparation of a medicine for improving brain cognitive impairment caused by low pressure and low oxygen. Research results show that low pressure and low oxygen can cause brain cognitive dysfunction of mice. The genistein can relieve brain dysfunction after low-pressure and low-oxygen exposure of mice by inhibiting neuroinflammation, recovering hippocampal neurological functions and improving hippocampal oxidative aggression. The invention provides a novel therapy for brain cognitive impairment caused by low pressure and low oxygen in various leguminous plant components, which is mainly derived from soy isoflavone by verifying a pharmaceutical active component, namely genistein.

The invention discloses a pharmaceutical composition and application thereof in preparation of a medicine for treating alcohol dependence, and belongs to the technical field of biological medicines. The pharmaceutical composition comprises effective amounts of epimedin B, genistein and sipeimine as effective components. The medicine composition has the characteristics of good curative effect, low toxic and side effects and the like. The medicine composition can effectively reverse the preference of alcohol-dependent animals on alcohol, improve the viability of human nerve cells under alcohol exposure, repair nerve cell damage caused by alcohol, regulate the expression quantity of ACHE genes and CHAT genes of cholinergic systems of the alcohol-dependent animals, improve the oxidative stress state caused by alcohol exposure, and improve the curative effect of alcohol-dependent animals on alcohol. The composition has little influence on food intake of alcohol-dependent animals, and can be used for treating alcohol dependence. The invention is helpful for promoting the research and development process of new drugs for alcohol dependence, and provides a new way for scientific development and utilization of resources of traditional Chinese medicinal materials such as herba epimedii and fritillaria fraxini.

The present invention relates to cosmetic application development field, there is provided a kind of soy isoflavones extract and the preparation method and application thereof of its composition. In the present invention, soy isoflavones raw material ethanol-water mixed solvent is diluted, macroporous resin is loaded and resin adsorption is carried out to saturation, obtains saturated resin;Then the saturated resin is carried out gradient elution, elution effluent is collected and concentrated, so as to obtain the soy isoflavones extract rich in genistein;And in the soy isoflavones extract rich in genistein (with dry weight basis), genistein content is up to more than 80%wt. In addition, in the present invention, with the genistein in soy isoflavones extract as main active ingredient, it is also possible to coordinate specific plant extract extract, obtain the compound composition with anti-wrinkle firming, nourishing effect, be highly suitable for preparing large health daily use or daily chemical products.

The invention discloses a functional solid beverage for women and a preparation method thereof, and belongs to the technical field of health food. Active components of the solid beverage consist of the following raw materials in parts by weight: 15-30 parts of fermented radix puerariae powder, 20-40 parts of germinated soybean powder, 10-20 parts of papaya powder, 10-20 parts of Chinese yam powder, 5-15 parts of a Chinese wolfberry extract, 5-15 parts of a red date extract, 1-3 parts of vitamin C and 10-25 parts of erythritol. The method is characterized in that the fermented radix puerariae powder is prepared by solid-state fermentation of radix puerariae through specific aspergillus niger, and the content of genistein is larger than or equal to 2.0%; the germinated soybean powder is prepared by germinating soybeans and drying at low temperature, and the germination rate is greater than or equal to 90%. Through a unique biotransformation pretreatment process, the content and bioavailability of key active ingredients are remarkably improved, and experiments prove that the combination of the fermented radix puerariae and the germinated soybeans has an unexpected synergistic effect in the aspects of promoting synthesis of collagen and hyaluronic acid and breast health; the product effect is far better than that of simply mixed conventional raw materials or commercially available similar products.

The invention discloses a special fertilizer for promoting growth of root nodules of albizia julibrissin and a preparation method thereof, the special fertilizer comprises: a pellet core comprising a phosphate fertilizer, an organic matter and a root nodule promoting substance, the pellet core comprises, by percentage, 37-42% of the phosphate fertilizer, 50-60% of the organic matter and 3-8% of the root nodule promoting substance, and the root nodule promoting substance comprises triacontanol, genistein and chitin; the pellet core is coated with a microbial agent for promoting the growth of the albizia julibrissin root nodule to form a microbial agent layer, and the colony count of the microbial agent is greater than or equal to 4 * 10 < 9 > CFU / mL; a film-forming agent is applied to the fungicide layer, a fungicide protection layer is formed on the fungicide layer, and the film-forming agent comprises one or more of a polyvinyl alcohol solution, a polyacrylic acid solution and a chitin solution. Compared with the prior art, by applying the special fertilizer, the number and average weight of root nodules of the albizia julibrissin are increased, meanwhile, sufficient phosphorus and carbon are provided for young forest of the albizia julibrissin, and the growth amount of the albizia julibrissin is increased.

The invention discloses an acacetin and piperazine eutectic, a preparation method, a composition and application thereof, and belongs to the technical field of medicines. Specifically, the invention discloses an eutectic substance formed by acacetin and piperazine, and the molecular formula of the eutectic substance is (C16H12O5). (C4H10N2). The invention relates to a preparation method of a cocrystal of acacetin and piperazine. The acacetin and piperazine eutectic is used as an active ingredient of a medicine and is applied to preparation of medicines for resisting oxidation, resisting inflammation, resisting viruses, resisting tumors, resisting rheumatism, easing pain, protecting the liver, regulating immunity, treating osteoporosis and treating cardiovascular and cerebrovascular diseases.

The application discloses a method for improving the proportion of genistein in soybean isoflavone glycosides through directional fermentation. Lactobacillus sp. The method is characterized in that Lactobacillus (RS-03027) is used for fermentation after induced pre-culture, and the substrate is fed in batches, so that high selective conversion of isoflavone glycosides to genistein is realized. The strain has high beta-glucosidase activity related conversion capacity, has been preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.21302. The process realizes high accumulation and high selective enrichment of the target product, effectively inhibits non-target aglycone, and has industrial amplification application potential.

According to the application research of the morinda citrifolia fruits in the medicine for inhibiting the gastric cancer cells, the application of the morinda citrifolia fermented juice in preparation of the ferroptosis inducer for inhibiting the gastric cancer cells is provided for the first time, and the morinda citrifolia fermented juice can inhibit tumor growth of a gastric cancer tumor mouse model. Through mass spectrometry analysis, the blood entering components comprise apigenin-7-O-glucuronide, genistein, naringenin-7-O-glucuronide, curcumol and the like, and the active component apigenin-7-O-glucuronide and the content of the apigenin-7-O-glucuronide are identified from morinda citrifolia for the first time. The morinda citrifolia fermented juice can be used as a ferroptosis inducer for inhibiting gastric cancer cells through a GPX4 / HO-1 signal channel, so that ferroptosis of the gastric cancer cells is caused. The invention provides application of morinda citrifolia in preparation of drugs for inhibiting gastric cancer cells, experiments show that morinda citrifolia juice has an apoptosis induction effect on SNU216 cells and MKN45 cells, and the SNU216 cells and MKN45 cells intervened by the morinda citrifolia juice do not have damage repair ability, influence on synthesis of cell DNA, promotion of cell apoptosis and occurrence of ferroptosis.

The invention provides a composition. The composition comprises genistein and formononetin, the content ratio of the genistein to the formononetin is (1): (3). According to the composition with the specific proportion, the hair fixing effect after hair transplantation is achieved, and after hair transplantation, hair can be subjected to a first-stage wound healing, a second-stage hair loss, a resting stage and a third-stage regrowth stage. In the process, formononetin mainly plays an anti-inflammatory role in the first stage, and promotes generation of Laminin in the second stage, so that the effects of stabilizing hair follicles and the like are achieved; genistein focuses on promoting hair to be converted from a resting stage to a growing stage by adjusting a GSK-3beta-beta-catenin signal channel in the second stage and inhibiting an AR receptor in the third stage so as to reduce sebum secretion. Due to different functions of the two components, the two components are combined together, so that a patient after hair transplantation can be more effectively nursed, and the hair fixing effect after hair transplantation is realized.