17 results about "Simvastatin" patented technology

This invention relates to extracts of traditional Chinese medicine, and particularly to the application of a compound extract with lipid-regulating effects in the preparation of lipid-regulating drugs. Experimental studies of this invention have found that a compound traditional Chinese medicine lipid-regulating formula, administered for 14 weeks, can effectively reduce serum total cholesterol levels in hyperlipidemic mice. Furthermore, the lipid-lowering effects of the compound formula at 7.08 g / kg (high-dose group) and 3.54 g / kg (low-dose group) in hyperlipidemic mice are similar to those of simvastatin. Moreover, the dosage of the lipid-regulating compound formula is lower. This important compound lipid-regulating formula described in this invention can be applied to the prevention and treatment of hyperlipidemia, providing a new option for the prevention and treatment of hyperlipidemia.

This invention obtains a stable ezetimibesimvastatin pharmaceutical composition by combining β-cyclodextrin and sulfite antioxidants. This pharmaceutical composition has better stability and satisfactory dissolution effect than the reference formulation. Furthermore, the preparation method of the ezetimibesimvastatin pharmaceutical composition of this invention is simple, the process does not require the use of organic solvents, and no special treatment is required, making it very suitable for industrial production.

The invention belongs to the technical field of medicine analysis and detection, and particularly relates to a high performance liquid chromatography method for related substances of a flutemevitamin three-component preparation. According to the method, a specific chromatographic column, a mobile phase type, a mobile phase proportion and a gradient elution procedure are used, and only when the parameters are met at the same time, good detection separation degree, sensitivity and specificity can be ensured, so that accurate and rapid detection of related substances in the flutemevir three-component preparation is realized; the successful application of the technology is helpful for improving the quality level of a medicinal preparation, ensuring the medication safety of a patient, and making a positive contribution to the development of the field of medicine research and development.

The invention belongs to the technical field of chromatographic detection, and provides a simvastatin detection method and application thereof. According to the detection method disclosed by the invention, qualitative and quantitative detection of simvastatin in a sample can be realized by adopting a high performance liquid chromatography-tandem mass spectrometry mode. In the high performance liquid chromatography detection, the simvastatin possibly existing in the red yeast rice powder and the product thereof can be detected by combining a pentafluorophenyl propyl chromatographic column and a formic acid methanol mobile phase. The detection method provided by the invention can effectively distinguish the statins and simvastatin naturally existing in the red yeast rice powder, and can effectively avoid the false positive result of simvastatin detection; the method can also be applied to detection of various products including capsules, tablets, oral liquid and the like containing the red yeast rice powder, and various quality monitoring scenes can be considered.

The invention provides a blood fat-reducing ganoderma lucidum spore powder pharmaceutical composition as well as a preparation method and application thereof, and belongs to the technical field of medicines. Comprising a ganoderma lucidum spore powder extract and a pharmaceutical composition, and the mass ratio of the ganoderma lucidum spore powder extract to the pharmaceutical composition is 10: (1-2); the pharmaceutical composition contains a simvastatin derivative and an apatamuron derivative. The prepared blood-fat-reducing ganoderma lucidum spore powder pharmaceutical composition adopts a mixed preparation of traditional Chinese medicine components and western medicine components, has complementary advantages, can quickly reduce cholesterol content and take effect quickly on one hand, can fundamentally inhibit cholesterol synthesis and improve metabolic disorder of hyperlipidemia on the other hand, and is small in side effect, high in patient compliance, good in effect and free of toxic and side effects on the other hand. Certain anti-inflammatory, anti-thrombotic and anti-tumor effects and the like are achieved, and the application prospect is wide.

This invention discloses a yeast strain with weakened acyl donor degradation, a modification method, and its application in the synthesis of simvastatin, belonging to the field of genetic engineering. It describes a synthetic pathway for heterologous expression of simvastatin using Pichia pastoris as the host, with exogenously added α-dimethylbutyryl- S methyl mercaptopropionate (DMB-) S Simvastatin biosynthesis is achieved using MMP as an acyl donor. This invention significantly reduces the Pichia pastoris's reliance on DMB- by knocking out genes 2, 19, and 18 in the Pichia pastoris genome. S Degradation efficiency of simvastatin. Recombinant strains mlow-SV(Δ2Δ19) and mlow-SV(Δ2Δ19Δ18) produced 7.94 mg / L and 10.37 mg / L simvastatin, respectively, after 72 h of shake-flask fermentation, representing increases of 201.4% and 293.6% compared to recombinant strain mlow-SV(Δku70).

The application discloses simvastatin-beta-cyclodextrin nanoparticles and a preparation method and application thereof, and belongs to the technical field of biological medicines, wherein the simvastatin-beta-cyclodextrin conjugate has active oxygen responsiveness, comprises an ester bond connection structure formed by a beta-cyclodextrin hydroxyl group, a thio-ketone connection unit and a simvastatin hydroxyl group, and the thio-ketone connection unit is a thio-ketone dicarboxylic acid residue formed by condensation of 3-mercaptopropionic acid and acetone; the simvastatin-beta-cyclodextrin conjugate can self-assemble to form simvastatin-beta-cyclodextrin nanoparticles. The active oxygen responsive simvastatin-beta-cyclodextrin conjugate provided by the application can dissociate in an oxidative stress related environment, realizes double-path cholesterol homeostasis regulation functions of inhibiting cholesterol synthesis and promoting cholesterol efflux, reduces the degree of macrophage foam, and further reduces the lipid load of an atherosclerotic plaque.

The invention provides a simvastatin microcrystal stock solution and a preparation method thereof as well as a simvastatin pharmaceutical composition and application thereof, and belongs to the technical field of pharmaceutical preparations. The simvastatin microcrystal stock solution comprises simvastatin microcrystals, a stabilizer and water. The preparation method of the simvastatin microcrystal stock solution comprises the following steps: dissolving the stabilizer in water, adding the simvastatin raw material medicine, uniformly stirring, and then carrying out wet grinding to obtain the simvastatin microcrystal stock solution. The simvastatin pharmaceutical composition comprises a simvastatin microcrystal stock solution and is obtained by diluting the simvastatin microcrystal stock solution with a solution, the simvastatin pharmaceutical composition has the characteristics of high stability and good redissolvability, the slow release effect is achieved after injection, and the effective blood concentration can be achieved with a low dosage.

The application belongs to the technical field of simvastatin sodium tablet production, in particular to a raw material feeding device for simvastatin sodium tablet production and processing, which comprises a shell, a mixing barrel, a driving assembly, a mounting assembly and a mixing assembly. The mixing barrel is rotatably connected to the inner bottom of the shell through a guide mechanism, and the driving assembly is fixedly connected to the bottom of the shell. The driving assembly comprises a driving motor, a main shaft and a mounting bracket. The mounting bracket is fixedly connected to the bottom of the shell, the driving motor is fixedly connected to the inner wall of the mounting bracket, the output end of the driving motor is fixedly connected with a worm, and the main shaft is fixedly sleeved with a worm wheel engaged with the worm. One end of the main shaft penetrates through the bottom of the shell and the mixing barrel and extends upward, and the inner top of the shell is fixedly connected with a support mechanism corresponding to the main shaft. The automatic mixing mechanism can quickly and fully mix the simvastatin sodium tablet processing raw materials, thereby effectively improving the use effect of the feeding device.

The invention relates to application of an ATG5-dependent STING activator to treatment of melanoma, simvastatin or pharmaceutically acceptable salt thereof is used for preparing a medicine for activating a cGAS-STING pathway and remodeling a melanoma immune microenvironment, dendritic cell maturation and antigen cross presentation are promoted, CD8 T cells are activated, the infiltration level of the CD8 T cells and the secretion ability of IFN-gamma and TNF-alpha are improved, and the melanoma immune microenvironment is improved. Meanwhile, the expression of depletion marker proteins PD-1, TIM3 and CD39 is down-regulated, so that the conversion from immune cold tumors to thermal tumors is realized. By activating a key cGAS-STING pathway in the dendritic cells, death of immunogenic cells can be efficiently induced, and maturation and antigen presentation of the dendritic cells are promoted, so that cold tumors are converted into hot tumors, the problem of drug resistance in immunotherapy is solved, and anti-tumor immune response is improved.

The application discloses a preparation method of glucose oxidase / simvastatin magnetic nanomotor, and steps are as follows: FeCl3.6H2O is added into deionized water and stirred, sodium citrate, urea and PAM are added, and after stirring, reaction is carried out in a Teflon autoclave to obtain Fe3O4 nano solution; PAA and ammonia water are added and stirred, and then isopropyl alcohol is added dropwise to obtain Fe3O4-PAA nano solution; tetraethyl orthosilicate is added and stirred, and then washing is carried out to obtain Fe3O4-PAA / mSiO2 nano solution; simvastatin is mixed with the nano solution to obtain Sim-loaded Fe3O4-PAA / mSiO2 nanoparticles; EDC and NHS solution are added and stirred, and then glucose oxidase is added, and finally glucose oxidase-simvastatin magnetic nanomotor is obtained; the method utilizes the driving of the magnetic nanomotor in the magnetic field and the catalytic reaction of GOx, enhances the targeting and permeability of the drug in the tumor, and the loaded simvastatin can affect the lipid metabolism of tumor cells and inhibit glucose uptake, and compared with the use of GOx alone, the anti-tumor activity is significantly improved.

A simvastatin albumin nanoparticle and preparation and use thereof. The simvastatin albumin nanoparticle is composed of simvastatin and serum albumin, wherein the mass ratio of simvastatin to serum albumin is 1:10-20. The simvastatin albumin nanoparticles are prepared by means of a nano-emulsification solvent volatilization method. The preparation method specifically comprises the preparation of an O / W emulsion and the preparation of albumin nanoparticles. The prepared nanoparticles have an average particle size of less than 500 nm, a polydispersity coefficient of less than 0.5, and an encapsulation efficiency of greater than 70%. The surface of the particles is negatively charged, and the zeta potential thereof is -31.25 mV. The nanoparticles can selectively target tumor cells, and exhibit different degrees of inhibitory effects on breast cancer, liver cancer, ovarian cancer, and colorectal cancer, with the strongest inhibitory effect observed on colorectal cancer.

The scheme discloses the field of biomedical materials and bone tissue engineering technology, and discloses a composite drug-loaded hydrogel and a preparation method and application thereof.The composite drug-loaded hydrogel comprises a self-assembled peptide hydrogel base, hollow mesoporous dopamine nanoparticles and simvastatin, the simvastatin is loaded in the hollow mesoporous structure of the hollow mesoporous dopamine nanoparticles to form SIM@H-MPDA drug-loaded nanoparticles, and the drug-loaded nanoparticles are stably dispersed in the three-dimensional network structure of RADA16-I.The preparation method of the composite drug-loaded hydrogel is also disclosed, and the preparation method comprises H-MPDA preparation, SIM@H-MPDA drug-loaded nanoparticle preparation and composite hydrogel assembly.The composite drug-loaded hydrogel has good injectability and in-situ gelation, can realize smooth and slow release of simvastatin, significantly promotes osteogenic differentiation and new bone formation, effectively repairs non-infectious bone defects, and has no adverse effects on main organs of the body.

The invention belongs to the technical field of medicines and disease treatment, and particularly relates to a traditional Chinese medicine composition for treating atherosclerosis and a preparation method and application thereof. The composition is prepared by taking rhizoma pinellinae praeparata, rhizoma coptidis, pericarpium trichosanthis, radix angelicae sinensis, rhizoma chuanxiong, ground beeltle, radix salviae miltiorrhizae and radix ginseng as raw medicinal materials and adding sucralose and maltodextrin. Wherein berberine and palmatine in coptis chinensis are core active components, and play a role in regulating blood fat, inhibiting inflammation and blocking foam cell formation. Researches show that the particles can significantly reduce the serum total cholesterol, triglyceride and low-density lipoprotein cholesterol levels of model animals, increase the high-density lipoprotein cholesterol level, reduce the aortic plaque area and inhibit RAW264.7 cell foaming, the curative effect is equivalent to that of simvastatin, and a new choice is provided for prevention and treatment of atherosclerosis.

The invention discloses an application of a raspberry tea extract in increasing the content of coenzyme Q10 in blood, and relates to the technical field of health-care food, the raspberry tea extract acts on rats eating high-fat food for a long time, the raspberry tea extract can promote synthesis of coenzyme Q10, and the content of coenzyme Q10 in the blood of the rats is remarkably increased. Meanwhile, the raspberry tea extract acts on rats which are subjected to long-term intragastric administration of simvastatin, synthesis of coenzyme Q10 can be promoted through the effect of the raspberry tea extract, and the content of coenzyme Q10 in blood of the rats is remarkably increased. Therefore, the raspberry tea extract can be used for preparing a product for increasing the content of the coenzyme Q10.

The application particularly relates to an artificial skin with antibacterial and inflammation regulating functions and a preparation method thereof, and belongs to the technical field of artificial skin. The artificial skin is obtained by combining a dermis layer formed by doping silver sulfadiazine and loading simvastatin polylactic acid microspheres in collagen as a carrier material with a silicone epidermis layer. The silver sulfadiazine loaded in the collagen matrix can effectively avoid bacterial infection, the slow-release function of the polylactic acid microspheres can release simvastatin in the later stage of skin regeneration, promote the conversion of macrophages to M2 type, participate in the anti-inflammatory and repair processes, and accelerate skin regeneration. The silicone epidermis layer can prevent the loss of body fluid and bacterial invasion.