2 results about "Amycolatopsis" patented technology

The application discloses a genetically engineered bacterium for producing A82846B, namely, an engineered bacterium in which endogenous genes GrtfA, Lfp, or a combination of GrtfA and Lfp are integrated in the genome of a starting bacterium Amycolatopsis orientalis. The application also discloses a construction method of the genetically engineered bacterium, and a method for fermenting the genetically engineered bacterium and obtaining A82846B from a fermentation liquor. The genetically engineered bacterium overexpresses the introduced endogenous genes GrtfA, Lfp, or the combination of GrtfA and Lfp, and produces A82846B, thereby improving the yield of A82846B and the proportion of A82846B in total products, achieving the positive effects of improving the production efficiency of the target product A82846B, reducing the production cost, and reducing environmental pollution.

This invention discloses *Amycolatopsis* sp., its culture, metabolites, bacterial agents, and applications. The *Amycolatopsis* sp. provided by this invention has strain number TRM77291 and accession number CCTCC M 2024327 at the China Center for Type Culture Collection. This strain exhibits antibacterial activity against Erwinia, Salmonella, *Escherichia coli*, and *Pseudomonas aeruginosa*. This invention is of significant importance for the control of Gram-negative bacterial infections and the research of novel actinomycete-derived drugs.