16 results about "Amycolatopsis" patented technology

To provide a plastic-degrading microorganism.SOLUTION: In one aspect, the present disclosure provides a microorganism capable of degrading plastic, belonging to a genus selected from the group consisting of the genus Acinetobacter, the genus Achromobacter, the genus Pseudomonas, the genus Serratia, the genus Amycolatopsis, and the genus Bacillus. In one aspect, the plastic to be degraded comprises at least one selected from polypropylene (PP), polyethylene (PE), polyvinylidene chloride (PVDC), and ethylene-vinyl alcohol copolymer (EVOH).SELECTED DRAWING: None

The invention provides a polylactic acid plastic degrading bacterium and application thereof, and belongs to the technical field of plastic degradation. The method comprises the following steps: separating and screening a potential functional strain which is capable of degrading polylactic acid plastic and is closer to an environmental background from a waste plastic stacked sludge sample in a garbage yard, and culturing by using polylactic acid as a culture medium of a unique carbon source and adopting a constant-temperature shaking culture method; the method comprises the following steps: carrying out tolerance and specific enrichment on potential microorganisms with a polylactic acid degradation function in an environmental sample, and meanwhile, separating and purifying bacterial colonies for multiple times by combining a dilution coating plate streaking method until pure bacteria are separated out. The Amycolatopsis nivea HY6 obtained through separation has a good degradation effect on polylactic acid plastics, and the degradation rate of the Amycolatopsis nivea HY6 on polylactic acid in 30 days is about 30%.

The present invention provides a method utilizing the deletion of the regulatory protein gene tyrR1 and the overexpression of the tyrA-aroF genes to improve vanillin yield through fermentation with glucose as the substrate in Amycolatopsis. Additionally, the invention introduces a recombinant Amycolatopsis strain with a high vanillin yield, which has knocked out the regulatory protein gene tyrR1 and the pyruvate kinase gene pyk. This strain also overexpresses the endogenous chorismate mutase / prephenate dehydrogenase bifunctional enzyme gene tyrA, the 3-deoxy-7-phosphoheptulonate synthase gene aroF, and the phosphoenolpyruvate synthase gene ppsA. This recombinant Amycolatopsis ferments glucose to produce vanillin, achieving a yield of 4.72 g / L, significantly higher than that of existing technologies.

The invention discloses a genetically engineered bacterium for producing 3-methoxy-4-hydroxybenzaldehyde as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of genetic engineering. According to the genetically engineered bacterium, a vanillin dehydrogenase vdh gene is knocked out from amycolatopsis, and exogenous isocitrate lyase aceA and malic acid synthetase aceB genes are expressed. Through the genetically engineered bacterium, an efficient 3-methoxy-4-hydroxybenzaldehyde synthesis route is constructed, and the 3-methoxy-4-hydroxybenzaldehyde is produced by using the strain, so that not only can the yield of the 3-methoxy-4-hydroxybenzaldehyde be increased, but also the fermentation period can be shortened. Therefore, the genetically engineered bacterium and the production method of the 3-methoxy-4-hydroxybenzaldehyde, provided by the invention, have good industrial application prospects.

The application discloses a genetically engineered bacterium for producing A82846B, namely, an engineered bacterium in which endogenous genes GrtfA, Lfp, or a combination of GrtfA and Lfp are integrated in the genome of a starting bacterium Amycolatopsis orientalis. The application also discloses a construction method of the genetically engineered bacterium, and a method for fermenting the genetically engineered bacterium and obtaining A82846B from a fermentation liquor. The genetically engineered bacterium overexpresses the introduced endogenous genes GrtfA, Lfp, or the combination of GrtfA and Lfp, and produces A82846B, thereby improving the yield of A82846B and the proportion of A82846B in total products, achieving the positive effects of improving the production efficiency of the target product A82846B, reducing the production cost, and reducing environmental pollution.

The present disclosure provides an isolated strain of Amycolatopsis sp. WGS_07 having accession number MCC 0218. The present disclosure provides a process for synthesizing furano-naphthoquinone and pyrazinone derivatives using a novel strain Amycolatopsis sp. . The novel compounds synthesized possesses activity against gram positive bacteria, malarial parasites, and various cancers.

The invention belongs to the technical field of microorganisms, and relates to an actinomycete strain and application thereof. The actinomycete strain is classified and named as amycolatopsis reevesii NVA54, the Latin name is Amycolatopsis reevesii, the preservation number of the actinomycete strain is CGMCC (China General Microbiological Culture Collection Center) 33989, and the preservation date is March 26, 2025. The invention further discloses a bacteriostatic agent containing the actinomycetes strain and application of the bacteriostatic agent and the actinomycetes strain. The actinomycete strain can obviously inhibit pathogenic bacteria of root rot, an obvious antibacterial zone is formed, and the antibacterial rate is not lower than 87.53%.

The present invention provides a fermentation system and a fermentation method for vancomycin, belonging to the technical field of microbial fermentation. The vancomycin fermentation system of the present invention comprises a basal medium and a feeding medium; the basal medium is glucose 10 - 30 g / L, peptone 5 - 15 / L, yeast extract 2 - 8 g / L, potassium dihydrogen phosphate 1 - 3 g / L, magnesium sulfate 0.5 - 1.5 g / L; the feeding medium is a glucose peptone mixture, glucose 500 g / L, peptone 250 - 1000 g / L; during the fermentation process, the feeding medium is added regularly. The present invention can effectively improve the fermentation ability of Amycolatopsis orientalis, increase the yield of vancomycin, and reduce the content of impurities - demethylvancomycin in the fermentation broth.

The present application belongs to the technical field of environmental pollution remediation, and particularly relates to a nano-composite preparation, a preparation method thereof and application. The nano-composite preparation provided by the present application comprises a nano material and an extracellular polymer solution of microorganisms, wherein the nano material is graphene oxide or a multi-walled carbon nanotube; and the microorganism is Amycolatopsis amylolytica HPD-2, which is preserved by the China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 2568. Compared with a single system, the nano material and the extracellular polymer system can improve the bioavailability of PAHs in soil, thereby improving the degradation of PAHs. The present application makes full use of the high surface activity of the nano material and the degradation characteristics of the extracellular polymer of the degrading bacteria, and the synergistic effect between the two significantly improves the degradation removal rate of PAHs in soil and water, thereby greatly shortening the remediation period of PAHs contaminated soil and water.

The application discloses a new Amycolatopsis qudensis species and application. The Amycolatopsis qudensis D2301 provided by the application has a preservation number of CGMCC No. 26389 in the China General Microbiological Culture Collection Center, and has staphylococcus aureus inhibiting activity. The application has important significance for controlling gram-positive bacterial infection, safe production and storage of green agricultural products, maintaining a healthy ecological environment of crops, and protecting the health of animals and plants.

The invention relates to the technical field of agricultural microorganisms, and in particular relates to a tea tree phyllostachys paratacidophilus BFI-L14 and application thereof. The invention discloses a tea tree interleaf paratyphagus acid bacterium BFI-L14, which is paratyphagus oryzae, and the preservation number of the tea tree interleaf paratyphagus acid bacterium BFI-L14 is CGMCC (China General Microbiological Culture Collection Center) No.35900. Volatile substances released by the strain have multiple effects of preventing and treating anthracnose of tea trees, promoting growth of arabidopsis thaliana and the like.

The invention belongs to the technical field of microorganisms, and particularly relates to application of Amycolatopsis pevumannii KC316, in particular to application of the Amycolatopsis pevumannii KC316 in preparation of a fungicide for promoting plant growth in a heavy metal polluted environment and improving the heavy metal enrichment capacity of plants, and the preservation number of the Amycolatopsis pevumannii KC316 is CGMCC No.26012. The invention further discloses a preparation method of the Amycolatopsis pevumannii KC316. Experiments prove that fermentation liquor of the amycolatopsis stutzmannii can promote the growth of the maidenhair in a high-cadmium and high-lead environment, improve the heavy metal enrichment capacity of the maidenhair in soil and improve the physiological indexes of plants, the problem of heavy metal contaminated soil can be further solved, secondary pollution to the environment is avoided, and the method is suitable for industrial production. The method is safer to human and livestock, and has important application value for ecological restoration and environmental protection.

The invention relates to the technical field of microbial medicines, and in particular relates to a novel angular anthracycline compound Thioangucycline A, which is separated from a fermentation extract of an actinomycete of Amycolatopsis and has anti-tumor and antibacterial activities. The invention also relates to a preparation method of the novel angular anthracycline compound Thioangucycline A. The preparation method of the novel angular anthracycline compound Thioangucycline A comprises the following steps of: separating the novel angular anthracycline compound Thioangucycline A; an in-vitro anti-tumor test shows that the compound Thioangucycline A has a very strong inhibition effect on a human cervical cancer cell HeLa and a human leukemia cell U937. An in-vitro antibacterial test shows that the compound Thioangucycline A has an obvious antibacterial effect on gram-positive bacteria staphylococcus aureus and bacillus subtilis, so that the compound can be used for preparing an anti-tumor or antibacterial drug. The invention provides a new lead compound for developing anti-tumor and antibacterial drugs, and provides a scientific basis for developing and utilizing microbial drug resources.

The invention discloses a Pictet-Spengler enzyme capable of identifying alpha-keto acid and application of the Pictet-Spengler enzyme capable of identifying the alpha-keto acid. According to the invention, a Pictet-Spengler enzyme gene apksB is obtained by cloning from actinomycetes NRRL B-65536T, the nucleotide sequence of the Pictet-Spengler enzyme gene apksB is as shown in SEQ ID NO.1, the full length of the Pictet-Spengler enzyme gene apksB is 960 bp, the amino acid sequence of the Pictet-Spengler enzyme gene apksB encoded by the Pictet-Spengler enzyme gene apksB is as shown in SEQ ID NO.2, and the Pictet-Spengler enzyme gene apksB contains 319 amino acids in total; the method comprises the following steps: cloning a Pictet-Spengler enzyme gene apkslB, connecting the Pictet-Spengler enzyme gene apkslB with an expression vector pET28a (+), then converting the Pictet-Spengler enzyme gene apkslB into escherichia coli BL21 (DE3), culturing, inducing expression, and purifying to obtain the Pictet-Spengler enzyme ApkslB which is used as a catalyst to catalyze the reaction of L-tryptophan and alpha-ketoglutaric acid so as to produce the tetrahydro beta-carboline skeleton kitasetasic acid. Discovery and functional identification of the Pictet-Spengler enzyme ApKslB have practical guiding significance on large-scale production of alkaloids, and the Pictet-Spengler enzyme ApKslB can be applied to the fields of biology, medicine, fine chemical engineering and the like.

The invention discloses a method for producing isoeugenol vanillin by using amycolatopsis, particularly relates to the technical field of microbial fermentation, and relates to a method for producing isoeugenol vanillin by using amycolatopsis. The isoeugenol vanillin produced by using the amycolatopsis is prepared from a recombinant strain and an isoeugenol solution; the recombinant strain is prepared from a recombinant plasmid and amycolatopsis; according to the invention, gene knockout is carried out at the position of a vanillin dehydrogenase gene, and isoeugenol monooxygenase is recombined and introduced to construct a recombinant gene, so that the target of efficiently converting isoeugenol into vanillin is achieved; according to the method, the amycolatopsis sp. With non-toxic substances and low vanillin degradation rate is adopted as a platform, and the isoeugenol monooxygenase is integrated into the genome of the amycolatopsis sp. Strain to construct a microbial synthesis chassis for high-yield isoeugenol vanillin, so that the green biosynthesis industrial production of vanillin is realized, and the cost is reduced.

This invention discloses *Amycolatopsis* sp., its culture, metabolites, bacterial agents, and applications. The *Amycolatopsis* sp. provided by this invention has strain number TRM77291 and accession number CCTCC M 2024327 at the China Center for Type Culture Collection. This strain exhibits antibacterial activity against Erwinia, Salmonella, *Escherichia coli*, and *Pseudomonas aeruginosa*. This invention is of significant importance for the control of Gram-negative bacterial infections and the research of novel actinomycete-derived drugs.