13 results about "Biotechnological process" patented technology

The invention relates to a biochemical method for preparing scopolamine, and belongs to the technical field of bioengineering. The invention aims to realize efficient conversion from scopolamine to scopolamine and improve the yield and industrial applicability of a target product. The invention provides a biochemical method for preparing scopolamine. The biochemical method comprises the following steps: adding scopolamine 6 beta hydroxylase into a culture medium containing L-scopolamine; the amino acid sequence of the hyoscyamine 6 beta hydroxylase is shown as any one of SEQ ID NO.1-5. The invention further discloses a preparation method of the hyoscyamine 6 beta hydroxylase. And the biochemical engineering method for realizing efficient and high-selectivity synthesis of scopolamine is realized.

The present invention relates to an apparatus for use in a biotechnological process, the apparatus comprising a vessel and a separator. The present invention also relates to a method for controlling flow of cells and culture medium between a vessel and a separator.

The utility model relates to the technical field of biochemical engineering extraction, and discloses biochemical engineering extraction equipment which comprises a base, a plurality of rolling rods are connected to the upper surface of the base in an embedded and rolling mode from left to right, the upper surfaces of the rolling rods are jointly and movably connected with a C-shaped base, and an extraction tank is fixedly connected to the interior of the C-shaped base in a penetrating mode. A shaking mechanism is arranged on the upper surface of the base and located on the right side of the rightmost rolling rod, and a supporting block is mounted above the extraction tank. An adjusting mechanism for driving the supporting block to lift is arranged on the upper surface of the extraction tank; the first motor in the shaking mechanism drives the turntable to rotate to drive the extraction tank to shake, so that the mixing of the biochemical engineering extract and the extraction agent is accelerated, meanwhile, the second motor drives the stirring rod to rotate, the mixing of the biochemical engineering extract and the extraction agent is further accelerated, and the adjusting mechanism drives the stirring rod to leave the material. The stirring rod is prevented from influencing the standing effect of the materials, and the influence on subsequent layering is also avoided.

A biotechnological process for producing PHA is described, comprising the steps of: subjecting an initial mixture containing a mixed bacterial mass to a biological selection process, so as to obtain a first intermediate mixture containing mainly selected bacteria able to store a carbonaceous source (for example VFA) in the form of PHA; introducing said first intermediate mixture in a tank reactor (120, 215) operating under anoxic con-ditions; introducing in said tank reactor (120, 215) also an aqueous nutrient solution, containing both the carbonaceous source (for example VFA) and nutrients, so as to ob-tain within said tank reactor (120, 215) a second intermediate mixture comprising PHA-containing bacteria; treating said second intermediate mixture with a solid-liquid separa-tion apparatus (125, 220), for example a vertical axis centrifuge, a decanter centrifuge or any other device adapted to execute a centrifugal separation of the second interme-diate mixture, so as to obtain a final mixture having a higher solids concentration with respect to that of the second intermediate mixture; subjecting the final mixture to a PHA extraction process.

The invention relates to a biotechnological process which comprises a bead milling step and / or an alkaline treatment step (process step a), and / or a subsequent enzymatic treatment step (process step b) and a cytolysis step (process step c) taking place after process step a) and / or after process step b). The invention further relates to the use of the components obtained by the biotechnological process as a nutrient composition, food product, food additive, pharmaceutical ingredient or nutrient medium for cell cultures.

Methods of producing sweet mogroside compounds via a biotechnological process are provided.SOLUTION: The present invention provides host cells and methods for producing mogrol glycosides, such as mogroside V (Mog. V), mogroside VI (Mog. VI), iso-mogroside V (Isomog. V), siamenoside, and glycosylation products that are minor products of Siraitiagrosvenorii. The present invention provides genetically engineered enzymes and genetically engineered host cells that produce mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, in high purity and / or high yield. Further, the present technology provides methods of making products, such as foods, beverages, oral care products, sweeteners, and flavors, comprising mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V.SELECTED DRAWING: None

A modular device for a fixed arrangement and interconnection of individual separation units and / or first functional units for performing one or more unit operations in a biotechnological process. The modular device includes a plurality of distributor caps provided for attachment to the separation units and / or first functional units. The distributor caps each have: a fluid distribution mechanism having a working connection and at least two supply or discharge connections, wherein the fluid distribution mechanism can assume at least two defined switching positions; connection mechanisms for establishing a flow communication between a fluid inlet or outlet of the separation unit or the first functional unit and the working connection of the fluid distribution mechanism; coupling mechanisms for establishing a rigid mechanical coupling and a flow communication with an adjacent distributor cap; and an interface for changing the switching positions of the fluid distribution mechanism.

A system (1000, 1000') comprising an area (101) for housing pharmaceutical, microbiological or biotechnological processes, a chamber (1, 1a) comprising walls (11) which delimit a volume (V) and at least an opening (12a), between the volume (V) and the area (101), for passage of objects and / or material (A) between the volume (V) and the area (101) and / or vice versa, and a vehicle (2) which is movable internally of the area (101), configured to move objects and / or materials (A) from and / or to the chamber (1, 1a) and comprising a door (21) which is engageable at least in an opening of the at least an opening (12a) so as to separate the other area (101) from the volume (V). The system (1000, 1000') enables realising a method for transferring objects and / or materials (A) and a pharmaceutical, microbiological or biotechnological process comprising the method.

A flow cell for optical spectroscopy is provided with a housing having a cavity which forms a measuring chamber, inlet and outlet channels and an optical window which seals an opening of the measuring chamber. The optical window is connected to the housing by melting a glass connecting element. A recess is formed around the opening and a supporting surface for the optical window is formed around the opening, so that the optical window contacts the housing at the supporting surface. The use of such a flow cell in processes monitoring of biotechnological processes and methods is also disclosed.

The present invention refers to a biotechnological process for obtaining suspensions of biogenic nanoparticles of zerovalent selenium, which have a high stability, and which have agronomic functions specific to plant biostimulants, that are amplified as a result of the formation of a biocorona which includes bioactive molecules from the biotechnological system wherein these selenium nanoparticles were formed. The biotechnological process involves the biosynthesis of selenium nanoparticles by the consortium of microalgae, Nannochloris sp. 424-1, deposited under CCAP number 251 / 10 in the Culture Collection of Algae and Protozoa (CCAP), and yeasts, Rhodotorula mucilaginosa TazRr, deposited under DSM number 34804, at the Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, to promote the formation of suspensions of stable selenium nanoparticles. The process also includes separation and concentration steps by micro- and ultrafiltration on ceramic membranes.

The invention relates to a biotechnological process for obtaining components from microorganisms with a cell wall, comprising a process step selected from the group consisting of a bead milling step, a high-pressure homogenization step, an ultrasound treatment step or a combination thereof (step a)), an enzymatic treatment step (step b)), and optionally a cytolysis step (step c)). The invention further relates to the use of the components obtained by the biotechnological process as nutrient composition, food product, food supplement, foodstuffs, food ingredients, pharmaceutical ingredients, feed ingredients, cosmetics, biofertilizer, soil additive or culture medium for cell cultures.