190 results about "Algal cells" patented technology

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

The invention relates to the technical field of wastewater treatment, and discloses a high-salinity wastewater treatment method and device for achieving bacterium-alga granulation and synergistic denitrification, the high-salinity wastewater treatment method for achieving bacterium-alga granulation and synergistic denitrification comprises the following steps: placing microalgae and aerobic granular sludge in a photobioreactor for initial culture; after initial culture, adding magnetic iron oxide nanoparticles into the photobioreactor, and carrying out enhanced culture, so as to obtain the algal-bacterial symbiotic aerobic granular sludge, and carrying out denitrification treatment on wastewater by utilizing the bacteria-algae symbiotic aerobic granular sludge. The magnetic iron oxide nanoparticles are added in the growth process of bacteria and algae, so that the problems of slow aggregation and nucleation of bacteria and algae, low activity of photosynthetic electron carriers of algae cells under high salt stress, reduction of denitrification efficiency and the like in the ABGS formation process can be effectively solved; rapid aggregation and nucleation of bacteria-algae cells can be synchronously enhanced, the electron transfer efficiency of an algae cell photosynthetic system under high salt stress is improved, and the denitrification efficiency of microorganisms is improved.

The invention relates to the technical field of red algae polysaccharide extraction, and provides a preparation technology for preparing instant red algae polysaccharide through a compound enzyme method, and the preparation technology comprises the following steps: S100, washing a red algae raw material, and sequentially carrying out mechanical crushing treatment and freeze drying treatment to obtain red algae powder; s200, sequentially performing enzymolysis treatment, centrifugal treatment and filtration treatment on the red algae powder to obtain supernatant liquid; and S300, sequentially carrying out concentration treatment, purification treatment, spray drying treatment and fluidization granulation treatment on the supernatant to obtain the red algae polysaccharide. The red algae raw material is efficiently separated and purified by adopting the compound enzyme, so that the efficient degradation of the red algae cell wall and the efficient extraction and preparation of the red algae polysaccharide are realized, a new technical way is provided for the efficient preparation and wide application of the red algae polysaccharide, and the method has important industrial application value and market prospect.

The invention relates to a microalgae photosynthetic carbon sequestration technology, and aims to provide a method for promoting microalgae carbon sequestration by a polyethylene glycol passivated nitrogen-doped carbon dot conversion spectrum. The method comprises the following steps: passivating the surfaces of nitrogen-doped carbon dots by using polyethylene glycol, and then directly adding the passivated nitrogen-doped carbon dots into a microalgae suspension, ultraviolet light with the wavelength of 280-390 nm in external light is converted into blue light with the wavelength of 400-500 nm by passivating the nitrogen-doped carbon dots so as to be directly utilized by microalgae, so that growth and carbon sequestration are promoted while the stress resistance of algae cells is improved. According to the method, damage to algae cells can be greatly reduced by converting an ultraviolet spectrum, and meanwhile, microalgae growth and carbon sequestration can be further promoted by converting ultraviolet light into blue light; the spectrum conversion effect is enhanced, the overall light energy utilization efficiency of microalgae carbon sequestration is improved, light inhibition is relieved, and the stress resistance of microalgae cells is improved, so that microalgae growth carbon sequestration is promoted, and the development of the microalgae carbon sequestration industry is promoted.

The invention discloses a wastewater comprehensive toxicity detection and water quality risk evaluation method which comprises the following steps: S1, determining an ammonia nitrogen index of a to-be-detected wastewater sample, and judging whether the to-be-detected wastewater needs to be diluted or not according to an ammonia nitrogen concentration value; s2, measuring and calculating the algae cell activity index value of the to-be-measured wastewater / diluted to-be-measured wastewater; s3, determining the water quality risk grade of the to-be-detected wastewater according to the algae cell activity index value of the to-be-detected wastewater. The method evaluates the water quality toxicity by determining the algae cell activity index, has the advantages of high speed, low cost and the like, and can be applied to comprehensive toxicity detection and water quality risk evaluation of industrial wastewater.

The invention provides a method for promoting synthesis of microcystis astaxanthin by adjusting the concentration of a stress substance, which comprises the following steps of: firstly, culturing microcystis algal bloom or microcystis aeruginosa to a logarithmic phase by adopting a BG11 culture medium under the conditions of specific temperature, illumination and shaking table rotating speed, and adjusting the cell density by centrifuging and resuspending; then, NaCl, KCl, beta-violet ketone, longifolene and H2O2 with different concentrations are added into the algae liquid respectively, control is set, through multi-dimensional index measurement, the algae cell density, the ROS level, the photosynthetic pigment content, the photosynthetic performance and the level of astaxanthin and a precursor thereof are monitored when treatment is carried out for 0 day, 2 days, 4 days and 6 days, the expression quantity of astaxanthin biosynthesis related genes is further analyzed, and the astaxanthin biosynthesis related genes are obtained. Therefore, the optimal concentration of NaCl, KCl, beta-violet ketone, longifolene and H2O2 is determined, the effect of promoting astaxanthin synthesis is compared, and a theoretical basis and a precise regulation and control method are provided for industrial production of astaxanthin by using microcystis.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

The invention discloses a method for enhancing biomass and astaxanthin accumulated by haematococcus pluvialis based on gradient salt stress, and belongs to the technical field of bioengineering. According to the method, low-salt pre-stress is firstly carried out in the haematococcus pluvialis logarithmic culture stage to enhance the stress resistance of algae cells, and then high-salt is applied to induce the haematococcus pluvialis to efficiently accumulate biomass and astaxanthin in the stress stage. According to the method, the biomass of haematococcus pluvialis and the yield and stress resistance of astaxanthin can be effectively improved, the process is simple, operation is easy, dependence on the environment is small, the environment cannot be harmed in the culture process, and the method has a strong application prospect in large-scale production.

The invention discloses a method for evaluating the environmental risk of perfluorooctane sulfonic acid by using euglena gracilis, and belongs to the field of environmental pollutant biological monitoring and ecotoxicology evaluation. The method comprises the following steps: detecting the photosynthetic activity and the movement ability of Euglena gracilis exposed to perfluorooctane sulfonic acid with environmental concentration and the organelle distribution condition of the perfluorooctane sulfonic acid in euglena gracilis cells in situ; the photosynthetic activity comprises in-situ monitoring of chlorophyll fluorescence parameters and maximum non-photochemical quenching coefficient of euglena gracilis, and quantitative analysis of chloroplast pigment content; the movement ability comprises horizontal light-driving movement speed and vertical light-driving movement speed measurement of the euglena gracilis and flagellum shedding rate calculation. The method breaks through the limitation of a traditional single autotrophic mode, reveals a regulation and control mechanism of nutritional conditions on toxic effects, provides a scientific basis for water environment safety monitoring and optimization, and has the advantages of being convenient to operate and low in cost.

The invention discloses a single-cell green alga and application thereof. The name of the single-cell green alga is Coelastrus sp. BS01, the preservation number is CCTCC NO: M 20251671, the preservation date is July 22, 2025, and the preservation unit is China Center for Type Culture Collection in Wuhan University, Wuhan, China. The single-cell green alga can grow in a selenium-polluted water body with relatively high concentration, can efficiently remove selenium in the water body and is enriched in alga cells, so that the single-cell green alga has the application potential of enriching and converting selenium.

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

The invention provides an efficient carbon sequestration microalgae culture pond, and belongs to the technical field of microalgae culture. Comprising a culture pond outer wall and a separation wall fixedly connected to the middle of the culture pond outer wall, a microalgae growth space composed of a first straight channel, a first bent channel, a second straight channel and a second bent channel is formed in the culture pond outer wall through the separation wall, and algae liquid is arranged in the microalgae growth space. When the device is used, the stirring assembly drives algae liquid to flow along the annular water channels of the first straight channel, the first bent channel, the second straight channel and the second bent channel, when the stirring assembly drives the algae liquid to the high position of the spoiler, the gravitational potential energy of the algae liquid is increased, and the algae liquid is converted into algae liquid kinetic energy through the gravitational potential energy in the flowing process; due to the fact that the spoilers are designed to have a certain gradient, when the algae liquid reaches a certain flow velocity, laminar flow is changed into turbulent flow, so that algae cells in the algae liquid are frequently exchanged up and down, the photosynthetic efficiency is enhanced, and absorption of carbon dioxide is promoted.

The microalgae phenol degradation and carbon fixation technology aims to provide a method for improving key enzymes to promote euglena to degrade phenol and enhance carbon fixation. The method comprises the following steps: adjusting the gene expression amount of three key enzymes, H+ transport ATPase, pyruvate decarboxylase and isocitrate dehydrogenase in euglena cells by performing phenol concentration gradient domestication on the euglena cells; performing large-scale culture growth on the euglena domesticated strains, adding appropriate phenol in the culture medium, and continuously feeding the gas containing CO2; calculating the carbon fixation rate of the euglena by measuring the carbon element content in the biomass of the algal liquid during the culture process; after the culture is completed, the euglena polysaccharide content in the unit volume of the algal liquid is measured, and the phenol content in the algal liquid is measured to calculate the phenol degradation rate. The euglena cells are domesticated by using the phenol concentration gradient, the gene expression amount of the key enzymes is improved by several times, the phenol degradation capacity of the euglena cells is enhanced, the carbon fixation rate is improved, and the polysaccharide content in the cells is also improved.

The invention discloses a green algae expression method and application of recombinant human serum albumin, and belongs to the technical field of biology. Aiming at the problems of high cost and incomplete post-translational modification of the existing recombinant protein expression system, the invention provides a technical scheme for efficiently expressing human serum albumin by using green algae. The method comprises the following steps: carrying out codon optimization on a human serum albumin gene sequence, introducing a recombinant vector into green algae cells, and screening transgenic algae strains through a culture medium containing a resistance agent, culturing the transgenic algal strain, splitting the algal cells and separating the recombinant albumin. According to the method, efficient production of the human serum albumin is achieved through a green alga expression system, and the product has a correct folding structure and biological activity and is mainly used for preparing medical plasma substitutes, drug carriers and biological agents.

The invention provides a preparation method of microalgae bait, the microalgae bait is prepared through microalgae culture, magnetic separation, microencapsulation wrapping, drying and other methods, during culture, a specific culture solution is added, culture conditions are adjusted, algae liquid cells are separated by adopting a nano magnetic dispersant, effective components in the microalgae cells are ensured, mixed cell sap is screened out, and the microalgae bait is prepared. The components in the core material are wrapped through microencapsulation wrapping, the components of the wall material are used for isolating the external environment, good oxidation resistance and water resistance are achieved, notches and pores of the bait are reduced after wrapping, the performance is stable, the storage life is long, the swelling and collapsing time in water is prolonged, collapsing of nutritional ingredients in the core material is delayed, and the bait has the advantages of being good in stability and good in stability. The loss rate is low, the acid value change rate is within 2.20%, the peroxide value change rate is within 1.41%, the water resistance time reaches 8.5 h, the loss rate is within 4.85%, the sinking rate reaches 98%, the bait is easy to sink into the water bottom and beneficial to being ingested by aquatic animals, and the utilization rate of the bait is increased.

This invention discloses a method for cultivating Spirulina, relating to the field of microalgae biotechnology, aiming to overcome the shortcomings of existing technologies in terms of low Spirulina biomass yield and protein content. The method includes: in a first cultivation stage, inoculating Spirulina into a basal culture medium and conducting photoautotrophic cultivation at a light intensity of 5000-10000 lux until the optical density (OD560) reaches 0.6-0.8; in a second cultivation stage, reducing the light intensity to 1000-3000 lux and adding calcium alginate-encapsulated sodium bicarbonate microspheres, cultivating until the algal cell dry weight reaches 1.5-2.0 g / L; in a third cultivation stage, increasing the light intensity to 8000-12000 lux and adding sodium nitrate slow-release granules supported on anion exchange resin. This invention can achieve a synergistic increase in Spirulina biomass dry weight and protein content.

Injectable photoelectrohydrogel for repairing retinal photoreceptor damage and its preparation method and application, photoelectroactive spirulina and conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) hydrogel are physically cross-linked to construct an injectable material with photoelectric response characteristics, and the function of damaged photoreceptor cells in the retina is repaired in situ. Sp as a biologically active component not only gives the material photoelectric signal conversion capability, but also can achieve local antioxidant stress function, PEDOT-based hydrogel forms a three-dimensional scaffold by physically wrapping Sp, which not only ensures the activity of algal cells but also has excellent charge and ion conduction performance. The hydrogel can be injected into the retina to construct a biomimetic electrochemical signal transmission interface, rebuild the signal transduction of damaged photoreceptor cells, and realize the in situ repair of retinal signal pathways and the partial recovery of visual function. This technology provides a new strategy for the curative treatment of retinal diseases such as blinding eye diseases, and has the advantages of minimally invasive intervention, excellent biocompatibility and customizable treatment area.

The invention belongs to the technical field of algae physiology, and particularly discloses a method for efficiently extracting microalgae chlorophyll, which comprises the following steps: a, taking an algae liquid sample, centrifuging, discarding supernatant, and adding 90% ethanol solution for resuspension; b, adding quartz sand into the resuspension, carrying out cell homogenization treatment under the condition of 60 Hz, repeating the cell homogenization treatment for 4 times, 45 seconds each time, and 5 seconds each time; and c, heating the sample in a constant-temperature water bath at 60 DEG C for 7 minutes, centrifuging, and collecting supernate. By optimizing the ethanol concentration, the cell homogenization time, the cell homogenization material and the hot bath time, the cell homogenization technology of combining an ethanol method with quartz sand broken algae cells is improved, the chlorophyll extraction efficiency is effectively improved, the stability is good, and large-scale application is easy. Compared with an acetone method, an ethanol method and an ethanol-steel ball method, the microalgae chlorophyll extraction method provided by the invention has obvious advantages.

A simpler and lower-cost method and associated hardware are described for recovering biopharmaceutical products from cell factories in higher yields by a liquid nitrogen-based pre-treatment of cell factories followed by gentler disintegration of cellular factories by tunable, pressurizing and de-pressurizing cycle with gaseous nitrogen. The cell factories are heterologous expression systems and could be based on plant cells, mammalian cells, algal cells, yeast cells or even bacterial cells. The pre-treatment step with liquid nitrogen softens the tougher cell wall structures thus enabling cells to be disrupted relatively easily during the gaseous nitrogen-based pressurizing / depressurizing cycle. This invention can enhance the yield of biopharmaceutical products from expression systems and reduce the overall cycle-time and further down-stream processing and polishing cost of biopharmaceutical products.

A method of recovering a product-of-interest from a plant or algal cell and a kit relating to the same are provided. The method of recovering a product-of-interest from a plant or algal cell includes (a) culturing a plant or algal cell that produces a product-of-interest in a culture medium; (b) adding functionalized nanoparticles to the culture medium, whereby the nanoparticles enter the plant or algal cell, bind the product-of-interest, and are extruded into the culture medium; (c) recovering the extruded nanoparticles from the culture medium; and (d) separating the product-of-interest from the extruded nanoparticles. The kit includes a plant or algal cell that produces a product-of-interest when in culture and a functionalized nanoparticle. Also provided is a method of separating plant or algal cells.

The present application relates to a kind of underwater lighting lamp assembly for microalgae culture, further relates to the photobioreactor comprising using the underwater lighting lamp assembly.The underwater lighting lamp assembly of the present application can realize 360 ° light source layout, and also can integrate stirring and aeration device, realize the illumination, stirring and aeration of photobioreactor and other functions.The underwater lighting lamp assembly of the present application can promptly take away the heat generated by lamp, reduce the temperature of lamp tube, reduce the influence of high temperature on light emitting and its service life, also can reduce the heat transfer of lamp tube heat to microalgae culture solution, reduce the temperature rise of microalgae culture system.The hyperboloid stirring paddle and its paddle face gas distribution hole used, mixing is uniform, gas-liquid contact is sufficient, reduce the damage of stirring shear to algal cell, and stirring energy consumption is low.In addition, the underwater lamp assembly of the present application is simple in structure, can be directly applied to large microalgae culture container, form photobioreactor, and easily remove, scale expansion and component replacement.

The present application relates to the technical field of water treatment, and particularly relates to a pretreatment method of high-algae raw water, a composite water purifying agent for high-algae raw water and a treatment method of high-algae raw water. The pretreatment method comprises the following steps: adding a water purifying agent A into high-algae raw water to be treated, wherein the water purifying agent A is a biomass charcoal material loaded with potassium permanganate; after a predetermined time interval, continuously adding a water purifying agent B into the high-algae raw water to complete the pretreatment, wherein the water purifying agent B comprises a water-soluble divalent iron salt, a water-soluble trivalent iron salt and chitosan. The method realizes mild pre-oxidation through the slow-release effect of the loaded oxidant, inactivates the algae cells but avoids rupturing; and combines the adsorption and the agglomeration and wrapping effect of the in-situ generated iron-manganese hydrate oxide to form stable composite particles. The process can efficiently remove the algae cells and algae-derived odor substances, significantly reduces the disinfection by-product generation potential, and the formed composite particles can strengthen the subsequent conventional coagulation, thereby saving the drug consumption.

The invention provides a method for removing hexavalent chromium ions in a water body, and relates to the technical field of treatment of wastewater containing heavy metal ions, and the method comprises the following steps: providing haematococcus pluvialis, activating the haematococcus pluvialis, and separating and purifying the haematococcus pluvialis; the method comprises the following steps: providing a culture medium and a hexavalent chromium ion-containing water body, adding the hexavalent chromium ion-containing water body into the culture medium to prepare the hexavalent chromium ion-containing culture medium, and culturing the purified haematococcus pluvialis by using the hexavalent chromium ion-containing culture medium. The purified haematococcus pluvialis grows on a culture medium containing hexavalent chromium ions, astaxanthin and beta carotene with high content are generated in cells, the cells have high reducing capacity, and high-valence Cr (VI) is reduced into Cr (III), so that the content of Cr (VI) in a water body is reduced, and the toxicity of high-valence chromium ions to algae cells is reduced.

This invention discloses a comprehensive toxicity evaluation and source tracing method for wastewater based on multi-indicator responses of algal cells. The method includes: adding the wastewater to be tested into an algal cell culture system for exposure and obtaining algal cell viability index values ​​at multiple exposure times; determining the pollution toxicity type of the wastewater based on a machine learning model and / or a multi-algal species combination detection method, and tracing the pollution source based on the pollution toxicity type; and evaluating the toxicity and risk of the wastewater based on the pollution toxicity type and algal cell viability index values ​​using a comprehensive toxicity evaluation system. This invention can identify toxicity types based on algal cell viability indicators and construct a comprehensive toxicity assessment system to comprehensively evaluate the toxicity and risk of wastewater, solving three major problems of existing technologies: static endpoint determination, neglect of species sensitivity differences, and missed detection of chronic toxicity.

The application discloses a kind of based on toxigenic and odor-producing gene toxigenic and odor-producing cyanobacteria field rapid monitoring system and method, a kind of based on toxigenic and odor-producing gene toxigenic and odor-producing cyanobacteria field rapid monitoring system, including water sample algal automatic enrichment module, algal cell rapid lysis and DNA extraction tube, isothermal / normal temperature PCR module, palm fluorescence detector, qualitative and quantitative algorithm and toxigenic and odor-producing cyanobacteria risk warning grade calculation software, mobile phone APP software, each module is sequentially linked.The application realizes the integrated detection of toxigenic and odor-producing cyanobacteria quickly, accurately and conveniently, solves the problem that existing technology cannot accurately distinguish between toxigenic and non-toxigenic cyanobacteria and is difficult to scientifically assess the health risk of water body, while overcoming the limitations of traditional monitoring technology, such as laboratory detection, large equipment, complex operation, long detection period, etc., and meeting the timeliness, convenience and ease of operation requirements of field scenes such as grassroots monitoring, outdoor inspection and emergency disposal of sudden water bloom.

This invention relates to the field of water treatment technology, specifically to a composite water treatment agent, its preparation method, and its application, comprising a slow-release disinfectant, aluminum chloride, ferric chloride, and polyacrylamide; the slow-release disinfectant comprises an adsorbent carrier and a disinfectant in a mass ratio of (7-9):1. Compared with existing technologies, the composite water treatment agent prepared by this invention does not contain phosphorus, significantly reducing the threat of eutrophication and greatly improving water quality. Iron ions can effectively remove aluminum ions from source water and residual free aluminum ions after aluminum salt coagulation. The slow-release disinfectant can gradually alter the morphology of algal cells, disrupting the stability of algal cell structure and thus killing algae. Compared with commonly used polyaluminum chloride, it reduces the amount of treatment agent added, thereby reducing the cost of wastewater treatment. The preparation method is simple and can be industrialized.

The embodiment of the present invention discloses a Haematococcus pluvialis LT4012, which has a collection number of GDMCC No. 65798 in the Guangdong Provincial Microbial Culture Collection Center. The Haematococcus pluvialis LT4012 can be used for any of the following applications: (A1) to increase the yield of astaxanthin; (A2) to prepare a product for producing astaxanthin; (A3) to produce astaxanthin under weak light; (A4) to prepare a product for inducing astaxanthin gene expression. The light-sensitive, high-yield astaxanthin Haematococcus pluvialis of the present invention has stable performance and is sensitive to strong and weak light signals. Compared with the original algae strain, the algae cells have strong stress resistance, significantly improved astaxanthin production rate, and stable yield.

The invention provides a muscle bundle, a biological hybrid robot and a preparation method of the biological hybrid robot. The muscle bundle comprises a driving module on the innermost layer, an oxygen and energy supply module on the innermost layer and serving as a middle layer and a barrier module on the outermost layer. All the modules are coaxially arranged; wherein the driving module comprises muscle cells, and the oxygen and energy supply module comprises algae cells. Muscle cells contained in the driving module contract to provide power for the robot; the oxygen and energy supply module generates oxygen, glucose and other nutrient substances by means of photosynthesis of algae and degradation of hydrogel; the barrier module has moisturizing and antibacterial effects and can protect internal muscles and algae cells. The biological hybrid robot comprises a muscle bundle and a skeleton structure, wherein the muscle bundle is attached to the skeleton structure. The biological mixing robot can effectively overcome the defects that an existing biological mixing robot cannot be separated from a liquid culture medium environment, energy sources are limited and the like, and the application scene and the application range of the biological mixing robot are expanded.