304 results about "Algal cells" patented technology

The invention discloses a floating algae flow type microimaging sheath fluid focusing micro-fluidic chip device and system, and belongs to the technical field of micro-fluidic control. According to the device, the three-channel sheath fluid flow chip design is adopted, a large-channel inlet structure is combined to avoid algae cell blockage, and a sheath flow focusing technology is utilized to compress a sample flow to the center of a channel and limit the sample flow within the field depth range of a microscope objective to obtain a clear image. The arc-shaped structure of the sample channel reduces laminar flow disturbance, and a four-side transparent observation area supports panoramic depth imaging. The system comprises a microscopic imaging module, a fluid driving module and a sheath liquid flow chip module, a flow rate ratio of sheath liquid flow to sample flow is accurately controlled through a dual-channel liquid flow driving injection pump, and stable imaging within the depth of field of an objective lens is realized by combining a manual focusing platform. Experimental verification shows that the system can effectively compress sample flow to 21m nuclear flow diameter, imaging of floating algae such as chlorella, scenedesmus quadricauda and the like is clear, and the contradiction between flux and imaging quality of a traditional micro-fluidic chip is solved.

The invention discloses a chlorella pyrenoidosa white mutant strain FZU65-W1 and application of the chlorella pyrenoidosa white mutant The classification name of the chlorella pyrenoidosa white mutant strain FZU65-W1 is chlorella pyrenoidosa, the chlorella pyrenoidosa white mutant strain is preserved in the China General Microbiological Culture Collection Center on September 11, 2024, the preservation number is CGMCC N0.46151, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with an original strain FZU65, the chlorella pyrenoidosa white mutant strain FZU65-W1 has the advantages that the growth speed of algae cells under the heterotrophic condition is higher, and meanwhile, the cells are white due to the fact that chlorophyll and carotenoid are not synthesized in the cells. The chlorella pyrenoidosa white mutant strain FZU65-W1 can be used for producing high-quality protein through heterotrophic fermentation, so that not only can a later-stage protein extraction and preparation process be simplified, but also the sensory acceptance of algae powder can be improved, and a high-quality germplasm resource is provided for industrial production and application of microalgae protein.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

The invention provides a haematococcus pluvialis culture medium and a culture method thereof. The culture medium comprises a nutrition proliferation culture medium, a stress induction culture medium and an accumulation strengthening culture medium which are used for segmented culture, the nutrition proliferation culture medium comprises sodium nitrate, monopotassium phosphate, potassium chloride, ferric ammonium citrate, sodium citrate and a trace element mixed solution; adding an osmotic stress agent, an oxidation inducer and plant hormones into a stress induction culture medium; and accumulating and strengthening the culture medium to remove sodium nitrate, and adding and supplementing a carbon source, a polymer penetrant and a zwitterionic stabilizer. The culture method comprises the following steps: a nutrition proliferation stage, a stress induction stage and an accumulation strengthening stage. According to the method, through a three-section culture medium and a fine regulation culture process, and through the synergistic effect of a composite stress factor and a dynamic environment signal, on the premise of ensuring relatively high activity of algae cells, bidirectional synergistic efficient improvement of biomass growth and astaxanthin accumulation is realized.

The invention relates to the technical field of wastewater treatment, and discloses a high-salinity wastewater treatment method and device for achieving bacterium-alga granulation and synergistic denitrification, the high-salinity wastewater treatment method for achieving bacterium-alga granulation and synergistic denitrification comprises the following steps: placing microalgae and aerobic granular sludge in a photobioreactor for initial culture; after initial culture, adding magnetic iron oxide nanoparticles into the photobioreactor, and carrying out enhanced culture, so as to obtain the algal-bacterial symbiotic aerobic granular sludge, and carrying out denitrification treatment on wastewater by utilizing the bacteria-algae symbiotic aerobic granular sludge. The magnetic iron oxide nanoparticles are added in the growth process of bacteria and algae, so that the problems of slow aggregation and nucleation of bacteria and algae, low activity of photosynthetic electron carriers of algae cells under high salt stress, reduction of denitrification efficiency and the like in the ABGS formation process can be effectively solved; rapid aggregation and nucleation of bacteria-algae cells can be synchronously enhanced, the electron transfer efficiency of an algae cell photosynthetic system under high salt stress is improved, and the denitrification efficiency of microorganisms is improved.

The invention discloses an experimental device and method for measuring the swimming characteristics of algae cells in shear flow. The experimental device comprises an air pressure source, a pressure pump, a liquid storage bottle, a flow meter, a micro-channel and a waste liquid bottle which are connected in sequence, the method comprises the following steps: determining average flow according to experimental working conditions to produce stable shear flow, shooting an algae cell movement track image sequence, obtaining the distribution of the migration velocity V of algae cells through the observed algae cell movement track, calculating the distribution of the flow velocity Vflow of the algae cells in combination with an analytical method, and calculating the flow velocity Vflow of the algae cells according to the distribution of the flow velocity Vflow of the algae cells. And calculating to obtain the algae cell swimming characteristics in the shear flow, wherein the algae cell swimming characteristics comprise the self swimming speed Vs and the angular speed omega. According to the device and the method, high-precision measurement of the movement behavior of the algae cells in the shear flow is realized, an experimental method and an analytical method are combined, the defect that swimming characteristics such as swimming speed and angular speed of the algae cells cannot be obtained in previous researches is overcome, and technical support is provided for development of micromechanics in a more detailed micro-scale.

The invention relates to the technical field of water treatment, in particular to a method for preparing a quaternized modified tannin-based organic flocculant through irradiation grafting, a product prepared through the method and application of the quaternized modified tannin-based organic flocculant. The method comprises the following steps: (1) under the irradiation of 30-60 mW / cm < 2 > of ultraviolet light, adding brominated vinyl triphenylphosphine into a mixture of tannic acid and a photoinitiator for reaction; (2) adjusting the ultraviolet intensity to 5-20 mW / cm < 2 > for reaction; (3) closing the ultraviolet reaction; and (4) precipitating the product with ethanol, filtering and washing to obtain the quaternary phosphonium modified tannin-based organic flocculant. Wherein the photoinitiator is selected from one of benzophenone and benzoin dimethyl ether. The quaternized modified tannin-based organic flocculant provided by the invention can efficiently remove algae in a water body in an environment-friendly manner, and avoids secondary pollution caused by algae cell rupture at the same time.

The invention relates to the technical field of red tide treatment, in particular to a method for treating red tide of globocystis globosa through combination of gel microsphere adsorption and ultrasonic treatment and a regeneration method of gel microballoon.The method for treating the red tide of globocystis globosa through combination of gel microsphere adsorption and ultrasonic treatment comprises the steps that firstly, gel microspheres are prepared; and then, filling the gel microspheres into a filter bag to jointly treat the spherical brown cystis red tide in the water area together with an ultrasonic device. In addition, according to the regeneration method of the gel microspheres, the gel microspheres subjected to adsorption treatment are recycled for regeneration and reuse, and the effect of removing the globocystis globosa is still good. According to the method for treating the red tide of the globocystis globocystis, through the adsorption of the gel microspheres and the ultrasonic-assisted combined effect, the globocystis globocystis cells in a water body can be efficiently removed, and release of intracellular hemolytic toxins of the globocystis globocystis cells is avoided; meanwhile, a good effect of eliminating phosphate in a water body is achieved, and resuscitation and re-outbreak of red tide algae cells are avoided.

The invention discloses a method for purifying tetracycline and efficiently producing oil by using microalgae in a cross-temperature zone, and belongs to the field of microalgae bioengineering. The invention aims to solve the technical problem of tetracycline pollution in the current cold region water environment. The method comprises the following steps: preparing a seed solution for microalgae culture, inoculating the seed solution into a BG-11 culture medium containing tetracycline, culturing until the logarithmic growth later stage of microalgae, centrifugally collecting algae cells, freeze-drying and weighing, and extracting grease in dry algae powder by utilizing an ultrasonication combined organic solvent method. The method disclosed by the invention is simple to operate, can be used for producing biological energy and adsorbing and degrading tetracycline under the low-temperature treatment condition by utilizing the microalgae, meanwhile, the stress resistance of the microalgae under the low-temperature condition is improved, the permeability of algae cells is regulated and controlled, and the production of the microalgae biological energy is promoted. The method disclosed by the invention is used for purifying tetracycline in cross-temperature zones and efficiently producing oil.

The invention provides a microalgae fertilizer capable of improving salt and alkali resistance of cucumbers, and the microalgae fertilizer is a composite microalgae bio-fertilizer and comprises scenedesmus obliquus, nitzschia grain and extracellular polymeric substances generated in the culture process of the nitzschia grain. The components of the microalgae compound fertilizer disclosed by the invention are all freshwater algae strains and natural active microalgae cells, so that sodium ions and metal cations cannot be accumulated, the microalgae compound fertilizer does not contain blue-green algae, and cytotoxin which is harmful to the environment cannot be generated; the used algae strains are easier to breed, and the biomass accumulation speed of the algae strains is far higher than that of other algae strains, so that the prepared microalgae biological fertilizer has the advantages of saving time and cost. And the polyunsaturated fatty acid generated by the microalgae fertilizer can enhance the plant resistance through an osmotic regulation system and an antioxidant enzyme ROS system.

The present invention provides a sunlight factory recirculating aquaculture system and method, belonging to the technical field of aquaculture. The sunlight factory recirculating aquaculture system includes a fish pond and a fiber rotary disk filtration system; the fiber rotary disk filtration system can remove large-particle-size particulate matters in the culture water and regularly harvest microalgae, and the treated water quality meets the fishery culture standard, and 97% of the culture water can be directly recycled. In the present invention, during the in-situ cultivation of microalgae and fish, the microalgae can convert nutrients such as CO2 exhaled by the fish and nitrogen and phosphorus in the culture water body into substances of the microalgae cells themselves, such as proteins, polysaccharides, vitamins, and lipids, and release oxygen, rapidly improving the culture water quality; the in-situ cultivation of microalgae and fish gives play to the advantage of the high photosynthetic efficiency of the microalgae, and the rapidly growing microalgae provides a suitable growth environment for the fish. The sunlight factory recirculating aquaculture system and method of the present invention can achieve high-density, high-stability, and low-energy-consumption recirculating aquaculture.

The invention relates to the technical field of red algae polysaccharide extraction, and provides a preparation technology for preparing instant red algae polysaccharide through a compound enzyme method, and the preparation technology comprises the following steps: S100, washing a red algae raw material, and sequentially carrying out mechanical crushing treatment and freeze drying treatment to obtain red algae powder; s200, sequentially performing enzymolysis treatment, centrifugal treatment and filtration treatment on the red algae powder to obtain supernatant liquid; and S300, sequentially carrying out concentration treatment, purification treatment, spray drying treatment and fluidization granulation treatment on the supernatant to obtain the red algae polysaccharide. The red algae raw material is efficiently separated and purified by adopting the compound enzyme, so that the efficient degradation of the red algae cell wall and the efficient extraction and preparation of the red algae polysaccharide are realized, a new technical way is provided for the efficient preparation and wide application of the red algae polysaccharide, and the method has important industrial application value and market prospect.

The invention relates to a microalgae photosynthetic carbon sequestration technology, and aims to provide a method for promoting microalgae carbon sequestration by a polyethylene glycol passivated nitrogen-doped carbon dot conversion spectrum. The method comprises the following steps: passivating the surfaces of nitrogen-doped carbon dots by using polyethylene glycol, and then directly adding the passivated nitrogen-doped carbon dots into a microalgae suspension, ultraviolet light with the wavelength of 280-390 nm in external light is converted into blue light with the wavelength of 400-500 nm by passivating the nitrogen-doped carbon dots so as to be directly utilized by microalgae, so that growth and carbon sequestration are promoted while the stress resistance of algae cells is improved. According to the method, damage to algae cells can be greatly reduced by converting an ultraviolet spectrum, and meanwhile, microalgae growth and carbon sequestration can be further promoted by converting ultraviolet light into blue light; the spectrum conversion effect is enhanced, the overall light energy utilization efficiency of microalgae carbon sequestration is improved, light inhibition is relieved, and the stress resistance of microalgae cells is improved, so that microalgae growth carbon sequestration is promoted, and the development of the microalgae carbon sequestration industry is promoted.

The invention discloses a wastewater comprehensive toxicity detection and water quality risk evaluation method which comprises the following steps: S1, determining an ammonia nitrogen index of a to-be-detected wastewater sample, and judging whether the to-be-detected wastewater needs to be diluted or not according to an ammonia nitrogen concentration value; s2, measuring and calculating the algae cell activity index value of the to-be-measured wastewater / diluted to-be-measured wastewater; s3, determining the water quality risk grade of the to-be-detected wastewater according to the algae cell activity index value of the to-be-detected wastewater. The method evaluates the water quality toxicity by determining the algae cell activity index, has the advantages of high speed, low cost and the like, and can be applied to comprehensive toxicity detection and water quality risk evaluation of industrial wastewater.

The invention provides a method for promoting synthesis of microcystis astaxanthin by adjusting the concentration of a stress substance, which comprises the following steps of: firstly, culturing microcystis algal bloom or microcystis aeruginosa to a logarithmic phase by adopting a BG11 culture medium under the conditions of specific temperature, illumination and shaking table rotating speed, and adjusting the cell density by centrifuging and resuspending; then, NaCl, KCl, beta-violet ketone, longifolene and H2O2 with different concentrations are added into the algae liquid respectively, control is set, through multi-dimensional index measurement, the algae cell density, the ROS level, the photosynthetic pigment content, the photosynthetic performance and the level of astaxanthin and a precursor thereof are monitored when treatment is carried out for 0 day, 2 days, 4 days and 6 days, the expression quantity of astaxanthin biosynthesis related genes is further analyzed, and the astaxanthin biosynthesis related genes are obtained. Therefore, the optimal concentration of NaCl, KCl, beta-violet ketone, longifolene and H2O2 is determined, the effect of promoting astaxanthin synthesis is compared, and a theoretical basis and a precise regulation and control method are provided for industrial production of astaxanthin by using microcystis.

The invention belongs to the technical field of molecular biology, and particularly relates to a method for screening algae cells. The method comprises the following steps: mixing a suspension of algae cells with a lipid dye for dyeing to obtain a dyed algae cell suspension; taking the dyed algae cell suspension as a water phase, and generating liquid drops from the water phase and the oil phase through a liquid drop micro-fluidic technology; and separating the liquid drops with the optical signals higher than the reference value, wherein the liquid drops contain the target algae cells. Through a liquid drop sorting method, a reaction system is improved to picoliter grade, finer control and operation are realized, high-flux and high-precision screening in a microenvironment is facilitated, and the problem that the reaction system is limited is solved. In addition, the screening cost of a single strain is also reduced. By adopting a method of firstly dyeing and then generating the liquid drops, omission possibly occurring in the fusion process of the liquid drops and the dye liquid drops is avoided, and fluorescence detection errors caused by different dye concentrations in the algae cell dyeing and liquid drop sorting process are avoided.

The invention relates to the technical field of ultrafiltration membranes, in particular to an SPDA modified MIL-101 modified polyether sulfone ultrafiltration membrane as well as a preparation method and application thereof. According to the invention, on the basis of a Michael addition reaction, MIL-101 nanoparticles are subjected to sulfonated dopamine modification, and the hydrophilic polyether sulfone (PES) membrane is prepared through a blending modification method. Firstly, an SPDA modified MIL-101 nanometer material is synthesized, a PES ultrafiltration membrane is modified through blending, and the filtering performance and the anti-pollution performance of algae cell extracellular organic matter (EOM) are improved. The influence of the SPDA modified MIL-101 nano material on the performance structure of the ultrafiltration membrane under different addition amounts is further analyzed. And pure water flux and pollution flux are used for evaluating the permeation and separation performance of the membrane. The anti-pollution performance of the membrane is evaluated by alternately filtering the EOM solution and the pure water in three cycles, the ratio of reversible pollution to irreversible pollution and the flux recovery rate.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

The invention discloses a method for enhancing biomass and astaxanthin accumulated by haematococcus pluvialis based on gradient salt stress, and belongs to the technical field of bioengineering. According to the method, low-salt pre-stress is firstly carried out in the haematococcus pluvialis logarithmic culture stage to enhance the stress resistance of algae cells, and then high-salt is applied to induce the haematococcus pluvialis to efficiently accumulate biomass and astaxanthin in the stress stage. According to the method, the biomass of haematococcus pluvialis and the yield and stress resistance of astaxanthin can be effectively improved, the process is simple, operation is easy, dependence on the environment is small, the environment cannot be harmed in the culture process, and the method has a strong application prospect in large-scale production.

The invention discloses a method for evaluating the environmental risk of perfluorooctane sulfonic acid by using euglena gracilis, and belongs to the field of environmental pollutant biological monitoring and ecotoxicology evaluation. The method comprises the following steps: detecting the photosynthetic activity and the movement ability of Euglena gracilis exposed to perfluorooctane sulfonic acid with environmental concentration and the organelle distribution condition of the perfluorooctane sulfonic acid in euglena gracilis cells in situ; the photosynthetic activity comprises in-situ monitoring of chlorophyll fluorescence parameters and maximum non-photochemical quenching coefficient of euglena gracilis, and quantitative analysis of chloroplast pigment content; the movement ability comprises horizontal light-driving movement speed and vertical light-driving movement speed measurement of the euglena gracilis and flagellum shedding rate calculation. The method breaks through the limitation of a traditional single autotrophic mode, reveals a regulation and control mechanism of nutritional conditions on toxic effects, provides a scientific basis for water environment safety monitoring and optimization, and has the advantages of being convenient to operate and low in cost.

The invention discloses an agricultural microalgae fertilizer in-situ manufacturing and applying device and method, and belongs to the field of microbial fertilizer manufacturing, the device comprises a first box body, the first box body is filled with a culture solution and microalgae, the first box body is communicated with a supplement pipe, the side wall of the first box body is provided with a plurality of overflow small holes, and the overflow small holes are communicated with the supplement pipe; the supplementing pipe can supplement a culture solution into the first box body, the supplementing pipe injects the culture solution into the first box body, and when the liquid level of the culture solution reaches the overflow small hole, the culture solution containing microalgae flows out to the soil, and when injection of the culture solution into the first box body is stopped, the microalgae remaining in the first box body continues to grow; the device is easy and convenient to operate, efficient preparation and application of the microalgae fertilizer can be achieved on the premise that the activity of microalgae cells is not damaged, the manufacturing and transportation cost of the microalgae fertilizer can be greatly reduced, the utilization efficiency of the fertilizer can be improved, and healthy growth of crops can be promoted.

The invention discloses a single-cell green alga and application thereof. The name of the single-cell green alga is Coelastrus sp. BS01, the preservation number is CCTCC NO: M 20251671, the preservation date is July 22, 2025, and the preservation unit is China Center for Type Culture Collection in Wuhan University, Wuhan, China. The single-cell green alga can grow in a selenium-polluted water body with relatively high concentration, can efficiently remove selenium in the water body and is enriched in alga cells, so that the single-cell green alga has the application potential of enriching and converting selenium.

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

The invention discloses a dosing system for removing algae through pre-chlorination enhanced coagulation and a refined intelligent regulation and control method, and belongs to the technical field of water treatment. The system comprises a raw water pump, a tubular static mixer, a flocculation basin, a sedimentation basin, an industrial personal computer and multiple online detectors, and is used for monitoring flow, temperature, turbidity, characteristic fluorescence peak intensity, ultraviolet absorbance and organic matter concentration. On the basis of integrated real-time sensing data, by monitoring the characteristic fluorescence intensity ratio of algae cells after pre-chlorination and before flocculation, the dosage of an oxidizing agent (available chlorine) and a coagulant is finely and intelligently regulated and controlled on the premise of ensuring the integrity of the algae cells and enhancing coagulation. Under different water quality conditions, the system shows good self-adaptive capacity, accurate adding of an oxidizing agent (available chlorine) and a coagulant is achieved by applying a dynamic adjustment algorithm, the effluent quality is guaranteed, and the medicine consumption is reduced.

The invention discloses application of flagellimonas sp. SN16, and relates to the field of application of microorganisms. The strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No. 65745. The culture supernatant has efficient algae-killing activity on free single cells and cysts of the globocystis globosa, and algae cells can be split after the culture supernatant is treated for 12 hours. In a red tide field experiment of the spherocystis globosa, the algae killing effect on the cysts is good, and the treated water body has no obvious biotoxicity. In addition, the algae-killing activity of the supernatant is kept stable in an environment with the temperature of-20 to 100 DEG C and the pH value of 2 to 12. The flagelmonas and the culture supernatant thereof provided by the invention have important application value in the field of biological treatment of the algal blooms of the globocystis globocystis, are expected to become an efficient and safe algicide, effectively treat the algal blooms of the globocystis globocystis and protect the marine ecological environment.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

The invention provides an efficient carbon sequestration microalgae culture pond, and belongs to the technical field of microalgae culture. Comprising a culture pond outer wall and a separation wall fixedly connected to the middle of the culture pond outer wall, a microalgae growth space composed of a first straight channel, a first bent channel, a second straight channel and a second bent channel is formed in the culture pond outer wall through the separation wall, and algae liquid is arranged in the microalgae growth space. When the device is used, the stirring assembly drives algae liquid to flow along the annular water channels of the first straight channel, the first bent channel, the second straight channel and the second bent channel, when the stirring assembly drives the algae liquid to the high position of the spoiler, the gravitational potential energy of the algae liquid is increased, and the algae liquid is converted into algae liquid kinetic energy through the gravitational potential energy in the flowing process; due to the fact that the spoilers are designed to have a certain gradient, when the algae liquid reaches a certain flow velocity, laminar flow is changed into turbulent flow, so that algae cells in the algae liquid are frequently exchanged up and down, the photosynthetic efficiency is enhanced, and absorption of carbon dioxide is promoted.

The present invention relates to an adhesion device (1) for culturing microalgae that increases the density of microalgae cultures and their biomass productivity. The device comprises a body (2) with an upper opening that contains a culture medium (3), which occupies half of its volume; a plurality of rotary discs (4) as a supporting surface on which the microalgae cells (5) adhere, promoting their growth to form a biofilm, said plurality of discs (4) being fixed to a horizontal rotation shaft (6), which passes through the body (2), and being equally spaced apart, the location of the rotation shaft (6) inside the body (2) being such that said discs remain semi-submerged in the culture medium (3); gas-liquid diffusers for gas transfer and bubbling in the culture medium (3); at least one moisture sensor and at least one temperature sensor for monitoring the moisture and temperature, respectively, of the gas phase inside the body (2) of the device (1); at least one temperature sensor for monitoring the temperature of the culture medium (3); an upper cover (7) on the upper opening of the body (2), which prevents the culture medium (3) and microalgae cells (5) from being exposed to the outside environment; a lighting board (8) arranged between each pair of discs (4) of the plurality of discs (4), being located on the rotation shaft (6) and arranged in slots (71) in the cover (7) so that said lighting boards (8) are not in contact with the inside of the body (2); and an electric motor to provide slow and accurate rotation of the rotation shaft (6), wherein the body (2) and the cover (7) are made of a transparent or translucent material that allows sunlight or artificial light to pass through.

The invention belongs to the technical field of cell surface engineering, and particularly relates to a preparation method of TA-Fe < 3 + > coated chlorella capable of improving biomass accumulation. According to the invention, chlorella cells are used as a coating object, a metal compound tannic acid-iron (III) (TA-Fe < 3 + >) is used as a coating material, and the shell has small influence on the physiological activity of the chlorella cells and has good biocompatibility. Compared with a natural chlorella cell, the surface of the chlorella cell is coated with a TA-Fe < 3 + > bionic shell, so that the viability and stability of the cell in a severe environment can be remarkably improved, the utilization rate of HCO3 <-> in the environment can be effectively improved through the synergistic effect between the shell and the cell, the biomass accumulation of the chlorella cell is further improved, and the biological activity of the chlorella cell is improved. And a new solution and possibility are provided for producing clean energy.