224 results about "Algal cells" patented technology

The invention discloses a floating algae flow type microimaging sheath fluid focusing micro-fluidic chip device and system, and belongs to the technical field of micro-fluidic control. According to the device, the three-channel sheath fluid flow chip design is adopted, a large-channel inlet structure is combined to avoid algae cell blockage, and a sheath flow focusing technology is utilized to compress a sample flow to the center of a channel and limit the sample flow within the field depth range of a microscope objective to obtain a clear image. The arc-shaped structure of the sample channel reduces laminar flow disturbance, and a four-side transparent observation area supports panoramic depth imaging. The system comprises a microscopic imaging module, a fluid driving module and a sheath liquid flow chip module, a flow rate ratio of sheath liquid flow to sample flow is accurately controlled through a dual-channel liquid flow driving injection pump, and stable imaging within the depth of field of an objective lens is realized by combining a manual focusing platform. Experimental verification shows that the system can effectively compress sample flow to 21m nuclear flow diameter, imaging of floating algae such as chlorella, scenedesmus quadricauda and the like is clear, and the contradiction between flux and imaging quality of a traditional micro-fluidic chip is solved.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

The invention provides a haematococcus pluvialis culture medium and a culture method thereof. The culture medium comprises a nutrition proliferation culture medium, a stress induction culture medium and an accumulation strengthening culture medium which are used for segmented culture, the nutrition proliferation culture medium comprises sodium nitrate, monopotassium phosphate, potassium chloride, ferric ammonium citrate, sodium citrate and a trace element mixed solution; adding an osmotic stress agent, an oxidation inducer and plant hormones into a stress induction culture medium; and accumulating and strengthening the culture medium to remove sodium nitrate, and adding and supplementing a carbon source, a polymer penetrant and a zwitterionic stabilizer. The culture method comprises the following steps: a nutrition proliferation stage, a stress induction stage and an accumulation strengthening stage. According to the method, through a three-section culture medium and a fine regulation culture process, and through the synergistic effect of a composite stress factor and a dynamic environment signal, on the premise of ensuring relatively high activity of algae cells, bidirectional synergistic efficient improvement of biomass growth and astaxanthin accumulation is realized.

The invention relates to the technical field of wastewater treatment, and discloses a high-salinity wastewater treatment method and device for achieving bacterium-alga granulation and synergistic denitrification, the high-salinity wastewater treatment method for achieving bacterium-alga granulation and synergistic denitrification comprises the following steps: placing microalgae and aerobic granular sludge in a photobioreactor for initial culture; after initial culture, adding magnetic iron oxide nanoparticles into the photobioreactor, and carrying out enhanced culture, so as to obtain the algal-bacterial symbiotic aerobic granular sludge, and carrying out denitrification treatment on wastewater by utilizing the bacteria-algae symbiotic aerobic granular sludge. The magnetic iron oxide nanoparticles are added in the growth process of bacteria and algae, so that the problems of slow aggregation and nucleation of bacteria and algae, low activity of photosynthetic electron carriers of algae cells under high salt stress, reduction of denitrification efficiency and the like in the ABGS formation process can be effectively solved; rapid aggregation and nucleation of bacteria-algae cells can be synchronously enhanced, the electron transfer efficiency of an algae cell photosynthetic system under high salt stress is improved, and the denitrification efficiency of microorganisms is improved.

The invention discloses an experimental device and method for measuring the swimming characteristics of algae cells in shear flow. The experimental device comprises an air pressure source, a pressure pump, a liquid storage bottle, a flow meter, a micro-channel and a waste liquid bottle which are connected in sequence, the method comprises the following steps: determining average flow according to experimental working conditions to produce stable shear flow, shooting an algae cell movement track image sequence, obtaining the distribution of the migration velocity V of algae cells through the observed algae cell movement track, calculating the distribution of the flow velocity Vflow of the algae cells in combination with an analytical method, and calculating the flow velocity Vflow of the algae cells according to the distribution of the flow velocity Vflow of the algae cells. And calculating to obtain the algae cell swimming characteristics in the shear flow, wherein the algae cell swimming characteristics comprise the self swimming speed Vs and the angular speed omega. According to the device and the method, high-precision measurement of the movement behavior of the algae cells in the shear flow is realized, an experimental method and an analytical method are combined, the defect that swimming characteristics such as swimming speed and angular speed of the algae cells cannot be obtained in previous researches is overcome, and technical support is provided for development of micromechanics in a more detailed micro-scale.

The invention relates to the technical field of water treatment, in particular to a method for preparing a quaternized modified tannin-based organic flocculant through irradiation grafting, a product prepared through the method and application of the quaternized modified tannin-based organic flocculant. The method comprises the following steps: (1) under the irradiation of 30-60 mW / cm < 2 > of ultraviolet light, adding brominated vinyl triphenylphosphine into a mixture of tannic acid and a photoinitiator for reaction; (2) adjusting the ultraviolet intensity to 5-20 mW / cm < 2 > for reaction; (3) closing the ultraviolet reaction; and (4) precipitating the product with ethanol, filtering and washing to obtain the quaternary phosphonium modified tannin-based organic flocculant. Wherein the photoinitiator is selected from one of benzophenone and benzoin dimethyl ether. The quaternized modified tannin-based organic flocculant provided by the invention can efficiently remove algae in a water body in an environment-friendly manner, and avoids secondary pollution caused by algae cell rupture at the same time.

The invention provides a microalgae fertilizer capable of improving salt and alkali resistance of cucumbers, and the microalgae fertilizer is a composite microalgae bio-fertilizer and comprises scenedesmus obliquus, nitzschia grain and extracellular polymeric substances generated in the culture process of the nitzschia grain. The components of the microalgae compound fertilizer disclosed by the invention are all freshwater algae strains and natural active microalgae cells, so that sodium ions and metal cations cannot be accumulated, the microalgae compound fertilizer does not contain blue-green algae, and cytotoxin which is harmful to the environment cannot be generated; the used algae strains are easier to breed, and the biomass accumulation speed of the algae strains is far higher than that of other algae strains, so that the prepared microalgae biological fertilizer has the advantages of saving time and cost. And the polyunsaturated fatty acid generated by the microalgae fertilizer can enhance the plant resistance through an osmotic regulation system and an antioxidant enzyme ROS system.

The present invention provides a sunlight factory recirculating aquaculture system and method, belonging to the technical field of aquaculture. The sunlight factory recirculating aquaculture system includes a fish pond and a fiber rotary disk filtration system; the fiber rotary disk filtration system can remove large-particle-size particulate matters in the culture water and regularly harvest microalgae, and the treated water quality meets the fishery culture standard, and 97% of the culture water can be directly recycled. In the present invention, during the in-situ cultivation of microalgae and fish, the microalgae can convert nutrients such as CO2 exhaled by the fish and nitrogen and phosphorus in the culture water body into substances of the microalgae cells themselves, such as proteins, polysaccharides, vitamins, and lipids, and release oxygen, rapidly improving the culture water quality; the in-situ cultivation of microalgae and fish gives play to the advantage of the high photosynthetic efficiency of the microalgae, and the rapidly growing microalgae provides a suitable growth environment for the fish. The sunlight factory recirculating aquaculture system and method of the present invention can achieve high-density, high-stability, and low-energy-consumption recirculating aquaculture.

The invention relates to the technical field of red algae polysaccharide extraction, and provides a preparation technology for preparing instant red algae polysaccharide through a compound enzyme method, and the preparation technology comprises the following steps: S100, washing a red algae raw material, and sequentially carrying out mechanical crushing treatment and freeze drying treatment to obtain red algae powder; s200, sequentially performing enzymolysis treatment, centrifugal treatment and filtration treatment on the red algae powder to obtain supernatant liquid; and S300, sequentially carrying out concentration treatment, purification treatment, spray drying treatment and fluidization granulation treatment on the supernatant to obtain the red algae polysaccharide. The red algae raw material is efficiently separated and purified by adopting the compound enzyme, so that the efficient degradation of the red algae cell wall and the efficient extraction and preparation of the red algae polysaccharide are realized, a new technical way is provided for the efficient preparation and wide application of the red algae polysaccharide, and the method has important industrial application value and market prospect.

The invention relates to a microalgae photosynthetic carbon sequestration technology, and aims to provide a method for promoting microalgae carbon sequestration by a polyethylene glycol passivated nitrogen-doped carbon dot conversion spectrum. The method comprises the following steps: passivating the surfaces of nitrogen-doped carbon dots by using polyethylene glycol, and then directly adding the passivated nitrogen-doped carbon dots into a microalgae suspension, ultraviolet light with the wavelength of 280-390 nm in external light is converted into blue light with the wavelength of 400-500 nm by passivating the nitrogen-doped carbon dots so as to be directly utilized by microalgae, so that growth and carbon sequestration are promoted while the stress resistance of algae cells is improved. According to the method, damage to algae cells can be greatly reduced by converting an ultraviolet spectrum, and meanwhile, microalgae growth and carbon sequestration can be further promoted by converting ultraviolet light into blue light; the spectrum conversion effect is enhanced, the overall light energy utilization efficiency of microalgae carbon sequestration is improved, light inhibition is relieved, and the stress resistance of microalgae cells is improved, so that microalgae growth carbon sequestration is promoted, and the development of the microalgae carbon sequestration industry is promoted.

The invention discloses a wastewater comprehensive toxicity detection and water quality risk evaluation method which comprises the following steps: S1, determining an ammonia nitrogen index of a to-be-detected wastewater sample, and judging whether the to-be-detected wastewater needs to be diluted or not according to an ammonia nitrogen concentration value; s2, measuring and calculating the algae cell activity index value of the to-be-measured wastewater / diluted to-be-measured wastewater; s3, determining the water quality risk grade of the to-be-detected wastewater according to the algae cell activity index value of the to-be-detected wastewater. The method evaluates the water quality toxicity by determining the algae cell activity index, has the advantages of high speed, low cost and the like, and can be applied to comprehensive toxicity detection and water quality risk evaluation of industrial wastewater.

The invention provides a method for promoting synthesis of microcystis astaxanthin by adjusting the concentration of a stress substance, which comprises the following steps of: firstly, culturing microcystis algal bloom or microcystis aeruginosa to a logarithmic phase by adopting a BG11 culture medium under the conditions of specific temperature, illumination and shaking table rotating speed, and adjusting the cell density by centrifuging and resuspending; then, NaCl, KCl, beta-violet ketone, longifolene and H2O2 with different concentrations are added into the algae liquid respectively, control is set, through multi-dimensional index measurement, the algae cell density, the ROS level, the photosynthetic pigment content, the photosynthetic performance and the level of astaxanthin and a precursor thereof are monitored when treatment is carried out for 0 day, 2 days, 4 days and 6 days, the expression quantity of astaxanthin biosynthesis related genes is further analyzed, and the astaxanthin biosynthesis related genes are obtained. Therefore, the optimal concentration of NaCl, KCl, beta-violet ketone, longifolene and H2O2 is determined, the effect of promoting astaxanthin synthesis is compared, and a theoretical basis and a precise regulation and control method are provided for industrial production of astaxanthin by using microcystis.

The invention belongs to the technical field of molecular biology, and particularly relates to a method for screening algae cells. The method comprises the following steps: mixing a suspension of algae cells with a lipid dye for dyeing to obtain a dyed algae cell suspension; taking the dyed algae cell suspension as a water phase, and generating liquid drops from the water phase and the oil phase through a liquid drop micro-fluidic technology; and separating the liquid drops with the optical signals higher than the reference value, wherein the liquid drops contain the target algae cells. Through a liquid drop sorting method, a reaction system is improved to picoliter grade, finer control and operation are realized, high-flux and high-precision screening in a microenvironment is facilitated, and the problem that the reaction system is limited is solved. In addition, the screening cost of a single strain is also reduced. By adopting a method of firstly dyeing and then generating the liquid drops, omission possibly occurring in the fusion process of the liquid drops and the dye liquid drops is avoided, and fluorescence detection errors caused by different dye concentrations in the algae cell dyeing and liquid drop sorting process are avoided.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

The invention discloses a method for enhancing biomass and astaxanthin accumulated by haematococcus pluvialis based on gradient salt stress, and belongs to the technical field of bioengineering. According to the method, low-salt pre-stress is firstly carried out in the haematococcus pluvialis logarithmic culture stage to enhance the stress resistance of algae cells, and then high-salt is applied to induce the haematococcus pluvialis to efficiently accumulate biomass and astaxanthin in the stress stage. According to the method, the biomass of haematococcus pluvialis and the yield and stress resistance of astaxanthin can be effectively improved, the process is simple, operation is easy, dependence on the environment is small, the environment cannot be harmed in the culture process, and the method has a strong application prospect in large-scale production.

The invention discloses a method for evaluating the environmental risk of perfluorooctane sulfonic acid by using euglena gracilis, and belongs to the field of environmental pollutant biological monitoring and ecotoxicology evaluation. The method comprises the following steps: detecting the photosynthetic activity and the movement ability of Euglena gracilis exposed to perfluorooctane sulfonic acid with environmental concentration and the organelle distribution condition of the perfluorooctane sulfonic acid in euglena gracilis cells in situ; the photosynthetic activity comprises in-situ monitoring of chlorophyll fluorescence parameters and maximum non-photochemical quenching coefficient of euglena gracilis, and quantitative analysis of chloroplast pigment content; the movement ability comprises horizontal light-driving movement speed and vertical light-driving movement speed measurement of the euglena gracilis and flagellum shedding rate calculation. The method breaks through the limitation of a traditional single autotrophic mode, reveals a regulation and control mechanism of nutritional conditions on toxic effects, provides a scientific basis for water environment safety monitoring and optimization, and has the advantages of being convenient to operate and low in cost.

The invention discloses a single-cell green alga and application thereof. The name of the single-cell green alga is Coelastrus sp. BS01, the preservation number is CCTCC NO: M 20251671, the preservation date is July 22, 2025, and the preservation unit is China Center for Type Culture Collection in Wuhan University, Wuhan, China. The single-cell green alga can grow in a selenium-polluted water body with relatively high concentration, can efficiently remove selenium in the water body and is enriched in alga cells, so that the single-cell green alga has the application potential of enriching and converting selenium.

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

The invention discloses a dosing system for removing algae through pre-chlorination enhanced coagulation and a refined intelligent regulation and control method, and belongs to the technical field of water treatment. The system comprises a raw water pump, a tubular static mixer, a flocculation basin, a sedimentation basin, an industrial personal computer and multiple online detectors, and is used for monitoring flow, temperature, turbidity, characteristic fluorescence peak intensity, ultraviolet absorbance and organic matter concentration. On the basis of integrated real-time sensing data, by monitoring the characteristic fluorescence intensity ratio of algae cells after pre-chlorination and before flocculation, the dosage of an oxidizing agent (available chlorine) and a coagulant is finely and intelligently regulated and controlled on the premise of ensuring the integrity of the algae cells and enhancing coagulation. Under different water quality conditions, the system shows good self-adaptive capacity, accurate adding of an oxidizing agent (available chlorine) and a coagulant is achieved by applying a dynamic adjustment algorithm, the effluent quality is guaranteed, and the medicine consumption is reduced.

The invention discloses application of flagellimonas sp. SN16, and relates to the field of application of microorganisms. The strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No. 65745. The culture supernatant has efficient algae-killing activity on free single cells and cysts of the globocystis globosa, and algae cells can be split after the culture supernatant is treated for 12 hours. In a red tide field experiment of the spherocystis globosa, the algae killing effect on the cysts is good, and the treated water body has no obvious biotoxicity. In addition, the algae-killing activity of the supernatant is kept stable in an environment with the temperature of-20 to 100 DEG C and the pH value of 2 to 12. The flagelmonas and the culture supernatant thereof provided by the invention have important application value in the field of biological treatment of the algal blooms of the globocystis globocystis, are expected to become an efficient and safe algicide, effectively treat the algal blooms of the globocystis globocystis and protect the marine ecological environment.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

The invention provides an efficient carbon sequestration microalgae culture pond, and belongs to the technical field of microalgae culture. Comprising a culture pond outer wall and a separation wall fixedly connected to the middle of the culture pond outer wall, a microalgae growth space composed of a first straight channel, a first bent channel, a second straight channel and a second bent channel is formed in the culture pond outer wall through the separation wall, and algae liquid is arranged in the microalgae growth space. When the device is used, the stirring assembly drives algae liquid to flow along the annular water channels of the first straight channel, the first bent channel, the second straight channel and the second bent channel, when the stirring assembly drives the algae liquid to the high position of the spoiler, the gravitational potential energy of the algae liquid is increased, and the algae liquid is converted into algae liquid kinetic energy through the gravitational potential energy in the flowing process; due to the fact that the spoilers are designed to have a certain gradient, when the algae liquid reaches a certain flow velocity, laminar flow is changed into turbulent flow, so that algae cells in the algae liquid are frequently exchanged up and down, the photosynthetic efficiency is enhanced, and absorption of carbon dioxide is promoted.

The present invention relates to an adhesion device (1) for culturing microalgae that increases the density of microalgae cultures and their biomass productivity. The device comprises a body (2) with an upper opening that contains a culture medium (3), which occupies half of its volume; a plurality of rotary discs (4) as a supporting surface on which the microalgae cells (5) adhere, promoting their growth to form a biofilm, said plurality of discs (4) being fixed to a horizontal rotation shaft (6), which passes through the body (2), and being equally spaced apart, the location of the rotation shaft (6) inside the body (2) being such that said discs remain semi-submerged in the culture medium (3); gas-liquid diffusers for gas transfer and bubbling in the culture medium (3); at least one moisture sensor and at least one temperature sensor for monitoring the moisture and temperature, respectively, of the gas phase inside the body (2) of the device (1); at least one temperature sensor for monitoring the temperature of the culture medium (3); an upper cover (7) on the upper opening of the body (2), which prevents the culture medium (3) and microalgae cells (5) from being exposed to the outside environment; a lighting board (8) arranged between each pair of discs (4) of the plurality of discs (4), being located on the rotation shaft (6) and arranged in slots (71) in the cover (7) so that said lighting boards (8) are not in contact with the inside of the body (2); and an electric motor to provide slow and accurate rotation of the rotation shaft (6), wherein the body (2) and the cover (7) are made of a transparent or translucent material that allows sunlight or artificial light to pass through.

The microalgae phenol degradation and carbon fixation technology aims to provide a method for improving key enzymes to promote euglena to degrade phenol and enhance carbon fixation. The method comprises the following steps: adjusting the gene expression amount of three key enzymes, H+ transport ATPase, pyruvate decarboxylase and isocitrate dehydrogenase in euglena cells by performing phenol concentration gradient domestication on the euglena cells; performing large-scale culture growth on the euglena domesticated strains, adding appropriate phenol in the culture medium, and continuously feeding the gas containing CO2; calculating the carbon fixation rate of the euglena by measuring the carbon element content in the biomass of the algal liquid during the culture process; after the culture is completed, the euglena polysaccharide content in the unit volume of the algal liquid is measured, and the phenol content in the algal liquid is measured to calculate the phenol degradation rate. The euglena cells are domesticated by using the phenol concentration gradient, the gene expression amount of the key enzymes is improved by several times, the phenol degradation capacity of the euglena cells is enhanced, the carbon fixation rate is improved, and the polysaccharide content in the cells is also improved.

The invention discloses a green algae expression method and application of recombinant human serum albumin, and belongs to the technical field of biology. Aiming at the problems of high cost and incomplete post-translational modification of the existing recombinant protein expression system, the invention provides a technical scheme for efficiently expressing human serum albumin by using green algae. The method comprises the following steps: carrying out codon optimization on a human serum albumin gene sequence, introducing a recombinant vector into green algae cells, and screening transgenic algae strains through a culture medium containing a resistance agent, culturing the transgenic algal strain, splitting the algal cells and separating the recombinant albumin. According to the method, efficient production of the human serum albumin is achieved through a green alga expression system, and the product has a correct folding structure and biological activity and is mainly used for preparing medical plasma substitutes, drug carriers and biological agents.

The invention provides a preparation method of microalgae bait, the microalgae bait is prepared through microalgae culture, magnetic separation, microencapsulation wrapping, drying and other methods, during culture, a specific culture solution is added, culture conditions are adjusted, algae liquid cells are separated by adopting a nano magnetic dispersant, effective components in the microalgae cells are ensured, mixed cell sap is screened out, and the microalgae bait is prepared. The components in the core material are wrapped through microencapsulation wrapping, the components of the wall material are used for isolating the external environment, good oxidation resistance and water resistance are achieved, notches and pores of the bait are reduced after wrapping, the performance is stable, the storage life is long, the swelling and collapsing time in water is prolonged, collapsing of nutritional ingredients in the core material is delayed, and the bait has the advantages of being good in stability and good in stability. The loss rate is low, the acid value change rate is within 2.20%, the peroxide value change rate is within 1.41%, the water resistance time reaches 8.5 h, the loss rate is within 4.85%, the sinking rate reaches 98%, the bait is easy to sink into the water bottom and beneficial to being ingested by aquatic animals, and the utilization rate of the bait is increased.

This invention discloses a method for cultivating Spirulina, relating to the field of microalgae biotechnology, aiming to overcome the shortcomings of existing technologies in terms of low Spirulina biomass yield and protein content. The method includes: in a first cultivation stage, inoculating Spirulina into a basal culture medium and conducting photoautotrophic cultivation at a light intensity of 5000-10000 lux until the optical density (OD560) reaches 0.6-0.8; in a second cultivation stage, reducing the light intensity to 1000-3000 lux and adding calcium alginate-encapsulated sodium bicarbonate microspheres, cultivating until the algal cell dry weight reaches 1.5-2.0 g / L; in a third cultivation stage, increasing the light intensity to 8000-12000 lux and adding sodium nitrate slow-release granules supported on anion exchange resin. This invention can achieve a synergistic increase in Spirulina biomass dry weight and protein content.

Injectable photoelectrohydrogel for repairing retinal photoreceptor damage and its preparation method and application, photoelectroactive spirulina and conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) hydrogel are physically cross-linked to construct an injectable material with photoelectric response characteristics, and the function of damaged photoreceptor cells in the retina is repaired in situ. Sp as a biologically active component not only gives the material photoelectric signal conversion capability, but also can achieve local antioxidant stress function, PEDOT-based hydrogel forms a three-dimensional scaffold by physically wrapping Sp, which not only ensures the activity of algal cells but also has excellent charge and ion conduction performance. The hydrogel can be injected into the retina to construct a biomimetic electrochemical signal transmission interface, rebuild the signal transduction of damaged photoreceptor cells, and realize the in situ repair of retinal signal pathways and the partial recovery of visual function. This technology provides a new strategy for the curative treatment of retinal diseases such as blinding eye diseases, and has the advantages of minimally invasive intervention, excellent biocompatibility and customizable treatment area.

The invention belongs to the technical field of algae physiology, and particularly discloses a method for efficiently extracting microalgae chlorophyll, which comprises the following steps: a, taking an algae liquid sample, centrifuging, discarding supernatant, and adding 90% ethanol solution for resuspension; b, adding quartz sand into the resuspension, carrying out cell homogenization treatment under the condition of 60 Hz, repeating the cell homogenization treatment for 4 times, 45 seconds each time, and 5 seconds each time; and c, heating the sample in a constant-temperature water bath at 60 DEG C for 7 minutes, centrifuging, and collecting supernate. By optimizing the ethanol concentration, the cell homogenization time, the cell homogenization material and the hot bath time, the cell homogenization technology of combining an ethanol method with quartz sand broken algae cells is improved, the chlorophyll extraction efficiency is effectively improved, the stability is good, and large-scale application is easy. Compared with an acetone method, an ethanol method and an ethanol-steel ball method, the microalgae chlorophyll extraction method provided by the invention has obvious advantages.