112 results about "Algal cells" patented technology

The invention relates to a microalgae photosynthetic carbon sequestration technology, and aims to provide a method for promoting microalgae carbon sequestration by a polyethylene glycol passivated nitrogen-doped carbon dot conversion spectrum. The method comprises the following steps: passivating the surfaces of nitrogen-doped carbon dots by using polyethylene glycol, and then directly adding the passivated nitrogen-doped carbon dots into a microalgae suspension, ultraviolet light with the wavelength of 280-390 nm in external light is converted into blue light with the wavelength of 400-500 nm by passivating the nitrogen-doped carbon dots so as to be directly utilized by microalgae, so that growth and carbon sequestration are promoted while the stress resistance of algae cells is improved. According to the method, damage to algae cells can be greatly reduced by converting an ultraviolet spectrum, and meanwhile, microalgae growth and carbon sequestration can be further promoted by converting ultraviolet light into blue light; the spectrum conversion effect is enhanced, the overall light energy utilization efficiency of microalgae carbon sequestration is improved, light inhibition is relieved, and the stress resistance of microalgae cells is improved, so that microalgae growth carbon sequestration is promoted, and the development of the microalgae carbon sequestration industry is promoted.

The invention discloses a single-cell green alga and application thereof. The name of the single-cell green alga is Coelastrus sp. BS01, the preservation number is CCTCC NO: M 20251671, the preservation date is July 22, 2025, and the preservation unit is China Center for Type Culture Collection in Wuhan University, Wuhan, China. The single-cell green alga can grow in a selenium-polluted water body with relatively high concentration, can efficiently remove selenium in the water body and is enriched in alga cells, so that the single-cell green alga has the application potential of enriching and converting selenium.

Systems and methods for developing and applying photosynthetic cellular substances to a human or animal for medical, therapeutic or cosmetic uses are provided. Photosynthetic cells, such as algal cells, can be used in these substances to provide the ability to continuously generate oxygen when exposed to a light source or other oxygen-generating trigger. The substances can be developed as a standalone liquid, gel or cream, or embedded within a bandage, mesh, scaffold or other structure with a light source to promote oxygen production. The substances can be applied topically for medical, therapeutic or cosmetic treatments, or injected internally for generation of oxygen within one or more parts of the body.

The microalgae phenol degradation and carbon fixation technology aims to provide a method for improving key enzymes to promote euglena to degrade phenol and enhance carbon fixation. The method comprises the following steps: adjusting the gene expression amount of three key enzymes, H+ transport ATPase, pyruvate decarboxylase and isocitrate dehydrogenase in euglena cells by performing phenol concentration gradient domestication on the euglena cells; performing large-scale culture growth on the euglena domesticated strains, adding appropriate phenol in the culture medium, and continuously feeding the gas containing CO2; calculating the carbon fixation rate of the euglena by measuring the carbon element content in the biomass of the algal liquid during the culture process; after the culture is completed, the euglena polysaccharide content in the unit volume of the algal liquid is measured, and the phenol content in the algal liquid is measured to calculate the phenol degradation rate. The euglena cells are domesticated by using the phenol concentration gradient, the gene expression amount of the key enzymes is improved by several times, the phenol degradation capacity of the euglena cells is enhanced, the carbon fixation rate is improved, and the polysaccharide content in the cells is also improved.

This invention discloses a method for cultivating Spirulina, relating to the field of microalgae biotechnology, aiming to overcome the shortcomings of existing technologies in terms of low Spirulina biomass yield and protein content. The method includes: in a first cultivation stage, inoculating Spirulina into a basal culture medium and conducting photoautotrophic cultivation at a light intensity of 5000-10000 lux until the optical density (OD560) reaches 0.6-0.8; in a second cultivation stage, reducing the light intensity to 1000-3000 lux and adding calcium alginate-encapsulated sodium bicarbonate microspheres, cultivating until the algal cell dry weight reaches 1.5-2.0 g / L; in a third cultivation stage, increasing the light intensity to 8000-12000 lux and adding sodium nitrate slow-release granules supported on anion exchange resin. This invention can achieve a synergistic increase in Spirulina biomass dry weight and protein content.

Injectable photoelectrohydrogel for repairing retinal photoreceptor damage and its preparation method and application, photoelectroactive spirulina and conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) hydrogel are physically cross-linked to construct an injectable material with photoelectric response characteristics, and the function of damaged photoreceptor cells in the retina is repaired in situ. Sp as a biologically active component not only gives the material photoelectric signal conversion capability, but also can achieve local antioxidant stress function, PEDOT-based hydrogel forms a three-dimensional scaffold by physically wrapping Sp, which not only ensures the activity of algal cells but also has excellent charge and ion conduction performance. The hydrogel can be injected into the retina to construct a biomimetic electrochemical signal transmission interface, rebuild the signal transduction of damaged photoreceptor cells, and realize the in situ repair of retinal signal pathways and the partial recovery of visual function. This technology provides a new strategy for the curative treatment of retinal diseases such as blinding eye diseases, and has the advantages of minimally invasive intervention, excellent biocompatibility and customizable treatment area.

A method of recovering a product-of-interest from a plant or algal cell and a kit relating to the same are provided. The method of recovering a product-of-interest from a plant or algal cell includes (a) culturing a plant or algal cell that produces a product-of-interest in a culture medium; (b) adding functionalized nanoparticles to the culture medium, whereby the nanoparticles enter the plant or algal cell, bind the product-of-interest, and are extruded into the culture medium; (c) recovering the extruded nanoparticles from the culture medium; and (d) separating the product-of-interest from the extruded nanoparticles. The kit includes a plant or algal cell that produces a product-of-interest when in culture and a functionalized nanoparticle. Also provided is a method of separating plant or algal cells.

The present application relates to a kind of underwater lighting lamp assembly for microalgae culture, further relates to the photobioreactor comprising using the underwater lighting lamp assembly.The underwater lighting lamp assembly of the present application can realize 360 ° light source layout, and also can integrate stirring and aeration device, realize the illumination, stirring and aeration of photobioreactor and other functions.The underwater lighting lamp assembly of the present application can promptly take away the heat generated by lamp, reduce the temperature of lamp tube, reduce the influence of high temperature on light emitting and its service life, also can reduce the heat transfer of lamp tube heat to microalgae culture solution, reduce the temperature rise of microalgae culture system.The hyperboloid stirring paddle and its paddle face gas distribution hole used, mixing is uniform, gas-liquid contact is sufficient, reduce the damage of stirring shear to algal cell, and stirring energy consumption is low.In addition, the underwater lamp assembly of the present application is simple in structure, can be directly applied to large microalgae culture container, form photobioreactor, and easily remove, scale expansion and component replacement.

The present application relates to the technical field of water treatment, and particularly relates to a pretreatment method of high-algae raw water, a composite water purifying agent for high-algae raw water and a treatment method of high-algae raw water. The pretreatment method comprises the following steps: adding a water purifying agent A into high-algae raw water to be treated, wherein the water purifying agent A is a biomass charcoal material loaded with potassium permanganate; after a predetermined time interval, continuously adding a water purifying agent B into the high-algae raw water to complete the pretreatment, wherein the water purifying agent B comprises a water-soluble divalent iron salt, a water-soluble trivalent iron salt and chitosan. The method realizes mild pre-oxidation through the slow-release effect of the loaded oxidant, inactivates the algae cells but avoids rupturing; and combines the adsorption and the agglomeration and wrapping effect of the in-situ generated iron-manganese hydrate oxide to form stable composite particles. The process can efficiently remove the algae cells and algae-derived odor substances, significantly reduces the disinfection by-product generation potential, and the formed composite particles can strengthen the subsequent conventional coagulation, thereby saving the drug consumption.

The invention provides a method for removing hexavalent chromium ions in a water body, and relates to the technical field of treatment of wastewater containing heavy metal ions, and the method comprises the following steps: providing haematococcus pluvialis, activating the haematococcus pluvialis, and separating and purifying the haematococcus pluvialis; the method comprises the following steps: providing a culture medium and a hexavalent chromium ion-containing water body, adding the hexavalent chromium ion-containing water body into the culture medium to prepare the hexavalent chromium ion-containing culture medium, and culturing the purified haematococcus pluvialis by using the hexavalent chromium ion-containing culture medium. The purified haematococcus pluvialis grows on a culture medium containing hexavalent chromium ions, astaxanthin and beta carotene with high content are generated in cells, the cells have high reducing capacity, and high-valence Cr (VI) is reduced into Cr (III), so that the content of Cr (VI) in a water body is reduced, and the toxicity of high-valence chromium ions to algae cells is reduced.

This invention discloses a comprehensive toxicity evaluation and source tracing method for wastewater based on multi-indicator responses of algal cells. The method includes: adding the wastewater to be tested into an algal cell culture system for exposure and obtaining algal cell viability index values ​​at multiple exposure times; determining the pollution toxicity type of the wastewater based on a machine learning model and / or a multi-algal species combination detection method, and tracing the pollution source based on the pollution toxicity type; and evaluating the toxicity and risk of the wastewater based on the pollution toxicity type and algal cell viability index values ​​using a comprehensive toxicity evaluation system. This invention can identify toxicity types based on algal cell viability indicators and construct a comprehensive toxicity assessment system to comprehensively evaluate the toxicity and risk of wastewater, solving three major problems of existing technologies: static endpoint determination, neglect of species sensitivity differences, and missed detection of chronic toxicity.

The application discloses a kind of based on toxigenic and odor-producing gene toxigenic and odor-producing cyanobacteria field rapid monitoring system and method, a kind of based on toxigenic and odor-producing gene toxigenic and odor-producing cyanobacteria field rapid monitoring system, including water sample algal automatic enrichment module, algal cell rapid lysis and DNA extraction tube, isothermal / normal temperature PCR module, palm fluorescence detector, qualitative and quantitative algorithm and toxigenic and odor-producing cyanobacteria risk warning grade calculation software, mobile phone APP software, each module is sequentially linked.The application realizes the integrated detection of toxigenic and odor-producing cyanobacteria quickly, accurately and conveniently, solves the problem that existing technology cannot accurately distinguish between toxigenic and non-toxigenic cyanobacteria and is difficult to scientifically assess the health risk of water body, while overcoming the limitations of traditional monitoring technology, such as laboratory detection, large equipment, complex operation, long detection period, etc., and meeting the timeliness, convenience and ease of operation requirements of field scenes such as grassroots monitoring, outdoor inspection and emergency disposal of sudden water bloom.

The invention belongs to the technical field of sewage treatment, and particularly relates to an algae removal process for algal bloom control by ultraviolet ozone and microbubbles. According to the invention, ultraviolet light catalysis, ozone oxidation and microbubble technologies are organically fused to form an efficient and synergistic algae removal system. The waterproof ultraviolet lamp is directly immersed in a water body, so that in-situ efficient production of ozone is realized, and a complex equipment system required by traditional ozone treatment is greatly simplified. The introduction of the microbubble technology significantly improves the gas-liquid mass transfer efficiency, and meanwhile, the generation of strong oxidizing active substances such as hydroxyl free radicals is effectively promoted by an instantaneous high-temperature and high-pressure microenvironment generated when microbubbles are broken in a water body. The system ingeniously combines triple advantages of ultraviolet inactivation, ozone oxidation and microbubble air floatation, and can synchronously degrade algal toxin and remove part of nutritive salt while realizing efficient removal of algal cells.

The present application relates to the technical field of water, wastewater, sewage or sludge treatment, and discloses a kind of drinking water source efficient and safe algicide based on hydroxyl iron modified clay and plant polyphenol, comprising: hydroxyl iron modified clay, plant polyphenol and porous carrier are uniformly mixed in anhydrous dry powder state, in raw water reaction medium, the oxygen atom of phenolic hydroxyl after deprotonation in plant polyphenol replaces the water molecules or mononuclear hydroxyl weak ligand of surface layer and surface of hydroxyl iron modified clay, and in situ assembly generates five-membered ring or six-membered ring surface iron polyphenol chelation network structure, and plant polyphenol is bonded and fixed in the inorganic framework surface of hydroxyl iron modified clay in solid phase insoluble state, the present application controls the organic carbon increment of effluent by surface iron polyphenol chelation network structure, so that uncoordinated phenolic hydroxyl presents outward exposure and forms affinity complex grid, and cooperatively completes algal cell aggregation and sedimentation and solid phase capture of free microcystin.

The present application relates to blue-green algae bloom control technical field, disclose a kind of sodium percarbonate combined with EM bacteria reinforced severe blue-green algae bloom control method, the present application uses sodium percarbonate combined with EM bacteria to control severe blue-green algae bloom, on the basis of adding sodium percarbonate 5mg / L further add EM bacteria 0.075mg / L, the algal cell density of experimental group is always lower than the control group of simply adding sodium percarbonate, and after adding sodium percarbonate 6h immediately further add EM bacteria, and the reducing effect is most remarkable, it shows that EM bacteria has the effect of reinforcing the effect of sodium percarbonate to control algae, and it is best to add EM bacteria after adding sodium percarbonate 6h, in the experimental group of adding EM bacteria 6h, algal cell density removal rate is 94.19% when experimental duration=48h, and the algal cell density removal rate of control group of only adding sodium percarbonate is only 69.77%.

This invention relates to the field of nucleic acid detection technology, specifically disclosing a liquid-phase nucleic acid chip for isothermal amplification of common algal blooms and its application. The method includes: collecting water samples and filtering to enrich algal cells; lysing the algal cells using cell lysis buffer to extract DNA; adding the extracted DNA to the liquid-phase nucleic acid chip for isothermal amplification; detecting the Ct value using a qPCR instrument; and determining the species and cell density of the algal bloom based on a preset standard curve. This invention directly quantifies algal cell density using algal gene copy number, with significantly higher accuracy than microscopic counting, and does not rely on the experience of professional personnel. It can be widely applied in frontline water resource management work.

The invention discloses an industrial circulating water algal inhibition process for in-situ generation of hydroxyl radicals, and relates to the technical field of industrial circulating water algal inhibition, a fluid kinetic energy and waste heat coordinated energy recovery unit is arranged on a water flow channel of an industrial circulating water system, and the fluid kinetic energy of circulating water is converted into electric energy; a tubular catalytic reactor is constructed, a composite catalytic electrode assembly containing a cathode and an anode is arranged in the tubular catalytic reactor, and an ultra-fine bubble generator is arranged at the water inlet end of the tubular catalytic reactor or in the reactor in series. Electric energy is converted through fluid kinetic energy of industrial circulating water, and external power grid high-grade electric energy does not need to be additionally relied on. Hydroxyl radicals are generated in situ in circulating water, and the hydroxyl radicals are high in oxidation activity, can continuously act on algae cells and are free of treatment blind areas; algae cell walls, chlorophyll and genetic materials can be effectively damaged, and algae are prevented from generating drug resistance; and the hydroxyl free radicals are rapidly quenched after reaction, no harmful by-product residue is left, and the algal inhibition effect and the environmental protection property are both considered.

The invention provides a marine microalgae rapid death-flocculating settling-seabed carbon storage method and shipborne equipment. The method comprises the following steps that a ship loaded with hydroxyl radical equipment is driven to a red tide outbreak sea area, a collector collects marine microalgae and pumps the marine microalgae into a main pipeline on the ship, and a drum of an enrichment device rotates at a high speed to enrich the microalgae; the OH solution is generated by the OH solution generation equipment and is injected into the liquid / liquid mixing and dissolving device formed by connecting the hydraulic cavitators in parallel to form a huge amount of liquid drops, the OH kills microalgae and mineralizes algal toxins at the moment of collapse of the liquid drops, and the morphology of algae cells is complete; conveying the dead algae to a flocculation tank, adding a flocculant-coagulant aid to form floccules with the dead algae, and settling at the position 3-5m below the sea surface along a main pipeline to realize carbon storage of the marine microalgae; according to the microalgae density and flow of the main pipeline, 1-5 gas-liquid mixing and dissolving devices of OH solution generation equipment are started, and 2-10 hydrodynamic cavitators and 1-5 flocculation tanks are correspondingly started; the central control system carries out fault diagnosis, prediction and early warning, intelligent monitoring and health management.

The invention belongs to the technical field of microorganisms, and particularly relates to a microvesicle bacterium capable of degrading sargassum horneri and application of the microvesicle bacterium. The microvesicles are microvesicles okinawensis, the microvesicles are preserved in the China General Microbiological Culture Collection Center on October 27, 2025, and the preservation number of the microvesicles is CGMCC (China General Microbiological Culture Collection Center) No. 36355. The microvesicle bacterium of the sargassum horneri component degrading enzyme is screened from rotten sargassum horneri, cell walls of the sargassum horneri can be efficiently cracked, release of effective components in brown algae is promoted, and therefore utilization of sargassum horneri resources is promoted.

The invention relates to a full-flow treatment process method for algae-laden water, which belongs to the technical field of sewage treatment and comprises the following steps: chloramine disinfection and pre-oxidation, humus treatment with magnetic resin, pH value adjustment with carbon dioxide for enhanced coagulation, flocculant coagulation, ferroferric oxide recovery with a magnetic separation device, filtration and disinfection. The chloramine and the magnetic resin cooperate with each other, so that the probability of algae cell rupture can be reduced, the adsorption efficiency of the magnetic resin is improved, the coagulation environment is optimized, chloramine is firstly used for disinfection and pre-oxidation and then the magnetic resin is used for treating humus during high algae-laden water sewage treatment, and the sequence of the method is very key. Chloramine is adopted for disinfection and pre-oxidation before magnetic resin adsorption, so that the probability of algae cell rupture is reduced, the magnetic resin adsorption efficiency is improved, blockage can be prevented, and the flow rate is increased.

This invention discloses a device for the synergistic pretreatment of algal cells by ultrasound and chlorination to inactivate them. The device includes a mixing reaction tank with an inlet pipe at one end, a chlorination oxidant dosing point connected to the inlet pipe, and an outlet pipe at the other end. It also includes an ultrasonic radiation device comprising a sound signal generator and a probe transducer electrically connected. The probe transducers are evenly arranged at the bottom of the reaction tank along the water flow direction and facing upwards; or the probe transducers are arranged on both sides of the mixing reaction tank walls, positioned below the liquid surface and facing the center of the water flow cross-section. This invention utilizes the synergistic effect of ultrasound and chlorination to effectively reduce chlorine dosage, increase algae removal rate, reduce water treatment costs, and improve the efficiency of subsequent processes, resulting in significant energy saving and emission reduction effects.

The invention relates to a pre-oxidation coupled coagulation algae removal method, and belongs to the technical field of water treatment.The method comprises the steps that ferrate is added into a water body for pre-oxidation, hydroxylamine hydrochloride is added into the water body after pre-oxidation, algae are flocculated and precipitated, and water-algae separation is achieved; according to the method, the ferrate and the hydroxylamine hydrochloride are combined to treat the algae-containing water body, the ferrate is utilized to pre-oxidize the extracellular organic matters of the algae cells, and by changing the composition of functional groups of the extracellular organic matters, the potential value of the algae liquid is remarkably reduced, and the algae cells are easy to settle. After the ferrate is pre-oxidized, hydroxylamine hydrochloride is added for reduction and stirring reaction, high-valence iron is rapidly reduced and decomposed to generate a large amount of Fe (OH) 3, under the combined action of adsorption, bridging, entrainment and the like, algae cells are agglomerated, flocs are generated and rapidly settled, and the flocs can be directly precipitated and removed from water.

Disclosed is a method for producing biocrude and bioactive substances from microalgae biomass. The method has the steps of mixing and stirring dry microalgae biomass with water and an acid catalyst, heating the mixture at high pressure at a temperature and time based on each individual species or processing the biomass mixture in a colloidal mill with solvent, separating lysed algae cells into non-polar extracts from water-soluble extracts via vacuum filtration, washing the lysed algae cells and non-polar extracts with organic solvents, separating the organic solvent mixture, which has ethanol and long-chain fatty acids, from the remaining biochar via vacuum filtration, and separating the ethanol from the fatty acids via distillation or rotary evaporator to extract bio-oil.

The application discloses a carrier unit, a removal device and a removal method for harmful algal blooms. The carrier unit comprises a biomimetic skeleton and adsorbing fibers. The biomimetic skeleton is a skeleton simulating deep-sea sponges. The skeleton comprises a frame body, the frame body is enclosed to form a cavity, the cavity extends along the length direction of the frame body and is through at both ends, the frame body is in a grid shape to form through holes in the frame body, the through holes are communicated with the cavity, and the outer wall of the frame body is provided with a plurality of raised ridge strips, the plurality of ridge strips are spirally arranged along the length direction of the frame body. The adsorbing fibers are filled in the cavity, the adsorbing fibers are carboxyl-containing fibers, and the adsorbing fibers are used for adsorbing algal cells in algal blooms. The application realizes physical separation of the structure supporting function and the adsorbing function, the skeleton can be repeatedly used for a long time, the fibers can be independently regenerated or replaced, and the defect that the whole is discarded after the function attenuation of the traditional integrated adsorbing material is overcome.

The application relates to a stress analysis method, system, device and medium for algal high-content images. The method comprises the following steps: acquiring an algal high-content image set, and performing quality screening control processing on the algal high-content image set to obtain algal high-content images that pass quality control; inputting the algal high-content images that pass quality control into a pre-trained algal exclusive segmentation model based on cellpose-sam to obtain algal cell image segmentation masks; based on the algal cell image segmentation masks, performing feature extraction on the algal high-content images to obtain feature vectors for algal and internal structures thereof; and inputting the feature vectors into a pre-trained binary classification stress analysis model to obtain a stress analysis result. The method can realize automatic analysis of the stress state of algae in algal high-content images.

The invention relates to the technical field of microalgae culture, in particular to an intelligent haematococcus pluvialis culture system and method for waste biogas slurry resource utilization, and the system comprises a biogas slurry collection module which is used for collecting waste biogas slurry; the biogas slurry treatment and culture medium preparation module is used for carrying out treatment and nutrition blending on waste biogas slurry to prepare a culture medium special for haematococcus pluvialis; the modular culture unit is used for bearing a special culture medium and culturing haematococcus pluvialis for green growth of algae cells; the intelligent control module is used for receiving real-time monitoring data in the breeding process and dynamically optimizing key parameters such as illumination, temperature, pH, gas flow and nutrition supply through a self-adaptive algorithm; the induction harvesting module is used for applying multiple stress to the high-density algae liquid to induce astaxanthin accumulation, and algae mud is collected through a secondary harvesting system. Therefore, the problems of high production cost, low culture efficiency and the like in the prior art are solved.

The application provides a method for evaluating ecological risk of microplastics based on population and community indexes, and belongs to the technical field of pollutant risk evaluation. The application studies the influence of microplastic concentration and particle size on the filter-feeding speed of Daphnia magna, analyzes the relationship between microplastic intake and algal morphology, and studies the influence of microplastics on the movement ability and filter-feeding of Daphnia magna after the microplastics are taken into the body, so as to evaluate the ecological risk of microplastics at a concentration far below the lethal concentration from the perspective of population and community. It is found for the first time that Daphnia magna will mistake microplastics similar in size to algal cells as food, and after the microplastics are taken into the body, the behavior characteristics of zooplankton will be affected, so that the harm of microplastics to zooplankton is comprehensively evaluated from the perspective of behavior and interspecific relationship. The application provides a new idea for evaluating ecological risk of microplastics from the perspective of population and community, and provides a basis for correctly formulating pollutant emission standards.

The application provides a dual-molecular ultraviolet synergistic algae removal method and application thereof. The algae removal method comprises the following steps: adding an algae-containing water body to be treated into an ultraviolet reaction device, and irradiating by using a first ultraviolet light source and a second ultraviolet light source; wherein the main emission peak wavelength of the first ultraviolet light source is 222 nm; the main emission peak wavelength of the second ultraviolet light source is 308 nm; and the algae-containing water body contains cyanobacteria. The algae removal method can be applied to water treatment, can realize rapid inactivation of algae cells, can significantly reduce the revival rate of algae cells, and can improve the durability of the algae removal effect and the stability of long-term algae control. The algae removal method provided by the application does not need to add any chemical agent, has the advantages of green environmental protection, simple operation, easy integration with existing water treatment processes, and the like, and has wide application prospect.

The application belongs to the technical field of microalgae concentration, and discloses a microalgae circulating precipitation concentration and recovery device and a recovery method thereof. The device comprises a light-shielded circulating precipitation concentration barrel, a liquid outlet pipe is connected to one side wall of the top of the circulating precipitation concentration barrel, a liquid inlet pipe is connected to one side wall of the circulating precipitation concentration barrel, and the liquid inlet pipe is below the liquid outlet pipe on the circulating precipitation concentration barrel; a power pump is connected to the outside of the liquid inlet pipe, and the water inlet end of the power pump is connected to an external algal liquid culture device through a pipeline. The liquid inlet flow is controlled by adjusting the power pump, so that the liquid surface rising speed in the circulating precipitation concentration barrel is less than the dark precipitation speed of algal cells. The algal cells are gradually precipitated and concentrated in the conical bottom of the circulating precipitation concentration barrel during the circulation of the algal liquid, so that the concentrated algal liquid can be recovered through the sampling pipe at the bottom of the circulating precipitation concentration barrel. The application realizes continuous circulating precipitation concentration of microalgae, has high concentration efficiency, and has low operation cost.

The application discloses a method for improving the algae removal effect of copper-loaded biochar, and comprises the following aspects: 1) taking corn stalks as raw materials, and preliminarily preparing original biochar through high-temperature anaerobic pyrolysis, and then loading copper sulfate on the carrier original biochar to obtain a copper-loaded biochar algae removal agent; 2) adding a certain amount of the copper-loaded biochar into a water body in which a Microcystis aeruginosa algal bloom breaks out, and inoculating a certain proportion of an inorganic salt solution; and 3) determining the best proportion of the inorganic salt by comparing the changes of indexes such as the algae cell removal rate. The application utilizes the inorganic salt ions to increase the particle size of the copper-loaded biochar, improves the contact efficiency of the copper-loaded biochar and algae, increases the salt stress and cell membrane damage of the algae cells, and further improves the algae removal efficiency. The application inoculates the inorganic salt solution into the Microcystis aeruginosa algal liquid to strengthen the algae removal effect of the copper-loaded biochar, and can be applied to the killing of harmful blue-green algae in lakes and sewage treatment plants.