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Food composition of microalgal biomass

A technology of composition and biomass, which is applied in the field of food composition of microalgae biomass, and can solve problems such as oxidation and instability of DHA

Inactive Publication Date: 2011-12-07
CORBION BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, DHA is not suitable for cooked food, because it will be oxidized by heat treatment
Additionally, DHA is unstable when exposed to oxygen even at room temperature and in the presence of antioxidants

Method used

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  • Food composition of microalgal biomass
  • Food composition of microalgal biomass
  • Food composition of microalgal biomass

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0241] Cultivate microalgae for high oil content

[0242] The microalgal strains were grown in shake flasks with the goal of obtaining more than 20% oil by dry cell weight. The medium in the flask used was as follows: K 2 HPO 4 : 4.2g / L, NaH 2 PO 4 : 3.1g / L, MgSO 4 ·7H 2 O: 0.24g / L, citric acid monohydrate: 0.25g / L, CaCl 2 2H 2 O: 0.025g / L, yeast extract: 2g / L and 2% glucose. The frozen cells were thawed at room temperature, 500ul of cells were added to 4.5ml of medium, and grown on a 6-well plate at 28°C for 7 days while stirring (200rpm). Dry cell weight was determined by centrifuging 1 ml of the culture at 14,000 rpm for 5 min in pre-weighed Eppendorf tubes. The culture supernatant was discarded, and the resulting cell mass was washed with 1 ml of deionized water. The culture was centrifuged again, the supernatant was discarded, and the cell pellet was placed at -80°C to freeze. Samples were then freeze-dried for 24 hours and the dry cell weight was calculated....

example 2

[0248] Three fermentation processes were performed with three different media formulations with the goal of producing algal biomass with a high oil content. The first preparation (medium 1) is based on the medium described by Wu et al. (1994Science in China, volume 37, phase 3, pages 326-335), and the composition per liter is as follows: KH 2 PO 4 , 0.7g; K 2 HPO 4 , 0.3g; MgSO 4 -7H 2 O, 0.3g; FeSO 4 -7H 2 O, 3mg; Thiamine hydrochloride, 10μg; Glucose, 20g; Glycine, 0.1g; H 3 BO 3 , 2.9 mg; MnCl 2 -4H 2 O, 1.8 mg; ZnSO 4 -7H 2 O, 220 μg; CuSO 4 -5H 2 O, 80 μg; and NaMoO 4 -2H 2 O, 22.9 mg. Second culture medium (medium 2) obtains the flask culture medium among the example 1, and the composition per liter is as follows: K 2 HPO 4 , 4.2g; NaH 2 PO 4 , 3.1g; MgSO 4 -7H 2 O, 0.24g; Citric Acid Monohydrate, 0.25g; Calcium Chloride Dehydrate, 25mg; Glucose, 20g; Yeast Extract, 2g. The third culture medium (medium 3) is mixed culture medium, and the compositio...

example 3

[0255] Preparation of biomass for food products

[0256] Microalgal biomass was produced by culturing microalgae as described in any of Examples 1-2. Microalgal biomass is harvested from fermenters, flasks, or other bioreactors.

[0257] Comply with GMP procedures. Whoever has or is suspected of having, by medical examination or by surveillance observation, a disease, an open lesion (including boils, sores, or infected wounds), or any other unusual source of microbial contamination, food, food-contact surfaces, or If there is a clear risk of contamination of food packaging material by such a source of microbial contamination, he will be prohibited from performing any operation that is expected to result in such contamination until the situation is corrected. Instruct personnel to report such medical conditions to their supervisor. All workers who come into direct contact with microalgal biomass, biomass contact surfaces, and biomass packaging materials are expected to obs...

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Abstract

The invention provides algal biomass, algal oil, food compositions comprising microalgal biomass, whole microalgal cells, and / or microalgal oil in combination with one or more other edible ingredients, and methods of making such compositions by combining algal biomass or algal oil with other edible ingredients. In preferred embodiments, the microalgal components are derived from microalgal cultures grown and propagated heterotrophically in which the algal cells comprise at least 10% algal oil by dry weight.

Description

[0001] Cross References to Related Applications [0002] This application requires U.S. Provisional Patent Application No. 61 / 105,121 filed October 14, 2008, U.S. Provisional Patent Application No. 61 / 157,187 filed March 3, 2009, and April 2009 as required by 35 U.S.C. 119(e) Benefit of U.S. Provisional Patent Application No. 61 / 173,166, filed September 27, and U.S. Provisional Patent Application No. 61 / 246,070, filed September 25, 2009. The entirety of each of the above applications is incorporated herein by reference for all purposes. [0003] Sequence Listing [0004] This application includes the accompanying Sequence Listing on pages 1-10. technical field [0005] The field of the invention is microbiology, food preparation and nutrition of humans and animals. Background technique [0006] As the population continues to grow, there is an increasing need for additional food sources, especially cheaply produced but nutritious food sources. In addition, the current rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23D7/005A23L7/109A23L15/00A23L19/00A23L23/00A23L27/60
CPCA23D7/0056A21D2/165A23K1/164A23K1/008A23D7/001A23D7/0053A23D7/003A23K10/16A23K20/158Y02A40/818Y02W90/10
Inventor G·布鲁克斯S·富兰克林J·阿维拉S·M·戴克尔E·巴利乌W·莱基特斯基J·皮朔基D·兹达尼斯
Owner CORBION BIOTECH INC
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