1766results about "Other foreign material introduction processes" patented technology

The present disclosure relates to systems, devices, and methods for electroporating. In an embodiment, the present disclosure relates to an electroporation system, comprising at least one electroporation chamber, each electroporation chamber comprising a first electrode and a second electrode, a plurality of ports comprising an inlet port at a first portion of each electroporation chamber, and an outlet port at a second portion of each electroporation chamber, a fluid conduit arranged between the first electrode and the second electrode, the fluid conduit being in fluid communication with the inlet port and the outlet port, and an automated system for electroporation operatively connected to the first electrode and the second electrode of the at least one electroporation chamber.

The invention discloses a method for improving beef quality, an action mechanism and an experimental method, in a cattle body, vitamin A can activate the expression of EBF2 through an active metabolite RA of the vitamin A, and the EBF2 can inhibit the transcription process of CYP26B1 in a targeted manner to maintain the activity of a retinol signal channel, so that PPAR gamma and downstream lipid metabolism related genes thereof are activated, and the activity of the retinol signal channel is improved. Fatty acid transport and lipid accumulation in fat cells in the cattle muscle are promoted, fat deposition in the cattle muscle is promoted, and the beef quality is improved. By constructing the molecular network for regulating and controlling the formation of the fat in the cattle muscle, a complex regulation and control mechanism for controlling the fat deposition in the cattle muscle can be understood more deeply, a method for improving the beef quality based on regulating and controlling the vitamin A mediated key factor EBF2 is provided, and a new way is provided for improving the beef quality in a targeted manner.

The invention discloses a cherax quadricarinatus oocyte targeted penetrating peptide VgSP and a living body delivery method thereof, and belongs to the technical field of biology. The invention provides the cherax quadricarinatus oocyte targeted penetrating peptide VgSP on one hand, and provides application of the cherax quadricarinatus oocyte targeted penetrating peptide VgSP on the other hand. The invention provides a polypeptide VgSP capable of being recognized by a cherax quadricarinatus oocyte surface receptor and mediated into an oocyte, and an injection and detection method of the polypeptide in sub-adult and adult female cherax quadricarinatus. The breakthrough lays a technical foundation for establishing a gene editing element delivery method with oocyte targeting, and is expected to be applied to the field of cherax quadricarinatus gene editing.

The invention discloses a target segment for preventing and treating tomato brown fruit wrinkling virus in tobacco, double-stranded RNA (Ribonucleic Acid) and application of the target segment and the double-stranded RNA. Wherein the nucleotide sequence of the target segment is as shown in SEQ ID NO.3, or a sequence which is as shown in SEQ ID NO.3, has at least 90% of homology and has coded amino acid as shown in SEQ ID NO.4 is substituted. The invention also discloses a double-stranded RNA of a targeted interference target section, and the double-stranded RNA can significantly inhibit the proliferation of the tomato brown crinkled fruit virus in tobacco by injecting or smearing tobacco leaves, so that the double-stranded RNA can be effectively applied to prevention and control of tobacco diseases caused by the tomato brown crinkled fruit virus. The method for preventing and controlling the tomato brown crinkled fruit virus in the tobacco by using the double-stranded RNA is safe, harmless and efficient, and provides a new path for preventing and controlling plant virus diseases by using RNAi (Ribonucleic Acid Interference).

Disclosed herein are engineered rhizobia having nif clusters that enable the fixation of nitrogen under free-living conditions, as well as ammonium and oxygen tolerant nitrogen fixation under free-living conditions. Also provided are methods for producing nitrogen for consumption by a cereal crop using these engineered rhizobia.

Aspects of the disclosure relate to complexes and other aspects relate to formulations (e.g., aqueous, lyophilized forms) comprising such complexes comprising an oligonucleotide (e.g., useful for targeting DMPK) covalently linked to an antibody (e.g., anti-TfR1 antibody).

Aspects of the disclosure relate to complexes and other aspects relate to formulations (e.g., aqueous, lyophilized forms) comprising such complexes comprising an oligonucleotide (e.g., useful for targeting DMPK) covalently linked to an antibody (e.g., anti-TfR1 antibody).

The invention discloses an interfering RNA (Ribonucleic Acid) for inhibiting a PCSK9 gene and application of the interfering RNA. The interfering RNA comprises any one or more than two nucleotide sequences as shown in SEQ ID NO: 1-40 and SEQ ID NO: 73-96. The interfering RNA can better target and silence mRNA of the liver PCSK9, reduce the protein level of the PCSK9, enhance LDL-C metabolism and reduce serum cholesterol, and a solid technical basis is provided for research and development of siRNA drugs for prevention and treatment of PCSK9 gene mediated diseases and symptom relief.

Provided are methods, compositions, and kits for improving a delivery of a nucleic acid to a cell in an organ of a subject. Such methods, compositions, and kits may include a sonoactive agent and a nucleic acid comprising a cargo polynucleotide and a nuclear localization element and / or an innate immune response avoidance moiety, which may be an aptamer. Delivery may involve sonoporation.

The invention belongs to the technical field of biological medicines, and particularly relates to a DNA tetrahedron capable of carrying oligonucleotides and nucleic acid analogues as well as a preparation method and application of the DNA tetrahedron. The method comprises the following steps: cutting off one single-chain DNA in classical tetrahedral framework nucleic acid to form three short-chain DNAs, and self-assembling six single-chain DNAs again to form a DNA tetrahedron to obtain ORT; according to the invention, the mismatching and self-matching phenomena of tFNA are improved, the yield of tFNA is improved, and the tFNA has good structural stability, high bioavailability and excellent biocompatibility; the ORT prepared by the invention can be used as a drug carrier for loading nucleic acid and nucleic acid analogues, protein drugs or small molecule drugs, and the application of the ORT in the aspects of drug delivery, gene therapy, biosensing and the like is promoted.

Provided are formulations, compositions and methods for delivering biological moieties such as modified nucleic acids into cells to modulate protein expression. Such compositions and methods include the delivery of biological moieties, and are useful for production of proteins.

Aspects of the disclosure relate to complexes and other aspects relate to formulations (e.g., aqueous, lyophilized forms) comprising such complexes comprising an oligonucleotide (e.g., useful for targeting DMPK) covalently linked to an antibody (e.g., anti-TfR1 antibody).

The invention belongs to the technical field of gene engineering, and discloses a CRISPR / Cas9 delivery system and application thereof. The CRISPR / Cas9 delivery system is obtained by loading the CRISPR / Cas9 system with the fluorinated polyethyleneimine, and the fluorinated polyethyleneimine can deliver the CRISPR / Cas9 carrier into cells, has excellent transmembrane delivery efficiency, and can efficiently mediate the CRISPR / Cas9 system to enter target cells. The CRISPR / Cas9 delivery system is applied to large-scale breeding of aquatic animals, the use safety is high, the biocompatibility is excellent, and the delivery system has excellent gene editing efficiency and has wide application prospects in the field of gene editing breeding.

Methods and compositions for modulating a target genome and stable integration of a transgene of interest into the genome of a cell are disclosed. Specifically, provided herein comprises compositions comprising an RNA molecule comprising a reverse complement sequence comprising an exogenous sequence and homology arms, an RNA molecule comprising an endonuclease, and one or more guide RNAs for specific genomic integration of the exogenous sequence.

The invention relates to a preparation method of core-shell nanoparticles with photo-thermal-phase change dual functions and intracellular delivery application of the core-shell nanoparticles, and belongs to the field of nano materials and cell delivery. The invention provides a photothermal-phase change bifunctional core-shell nanoparticle applied to intracellular delivery of biomacromolecules, the nanoparticle is of a core-shell structure, a high-molecular polymer is taken as a shell, liquid fluorocarbon and a photothermal material are taken as a core, the nanoparticle is prepared by an ultrasonic emulsification method, and the preparation method is simple. The boiling point of the liquid fluorocarbon is low, and the photo-thermal material has good photo-thermal conversion performance, so that the core-shell nanoparticles can quickly generate photo-thermal steam microbubbles under laser induction. Under the synergistic effect of laser and core-shell nanoparticles, generated steam microbubbles are induced to generate micropores in plant cell walls and cell membranes or animal cell membranes, so that the physical barrier of the cell walls and the cell membranes is overcome, and the efficiency of delivering exogenous functional biomacromolecules into cells is remarkably improved.

A method of culturing cells, the method including providing a microfluidic device including a substrate including an array of grooves that are substantially parallel; introducing cells into one or more of the grooves; providing a flow of culture medium over the groove array in a first flow direction that is across the grooves; culturing the cells under conditions to support viability, growth or differentiation, optionally wherein: (i) the cells are introduced into one or more grooves by flow over the groove array in the first flow direction; and / or (ii) the flow of culture medium is provided at a flow rate equivalent to 0.2-5 mL per million cells per day; and / or (iii) the cells are cultured for 1-14 days.

The present invention concerns engineered phagemids and methods for antibacterial peptide delivery against bacterial pathogens, in particular Clostridioides difficile.

To provide novel transduction enhancers and uses thereof.SOLUTION: The present invention relates to a method for transducing a target cell, the method comprising the step of contacting a target cell with a retroviral vector and a compound capable of enhancing transduction efficiency or a combination of such compounds, wherein the target cell is pre- and / or co-stimulated by pre- and / or co-incubation with a transduction enhancing compound or a combination of transduction enhancing compounds prior to and / or during contacting the target cell with the retroviral vector.SELECTED DRAWING: None

The present invention relates to chirally controlled oligonucleotides of select designs, chirally controlled oligonucleotide compositions, and methods of making and using the same. In some embodiments, a provided chirally controlled oligonucleotide composition provides different cleavage patterns of a nucleic acid polymer than a reference oligonucleotide composition. In some embodiments, a provided chirally controlled oligonucleotide composition provides single site cleavage within a complementary sequence of a nucleic acid polymer.

A method for simultaneously regulating the expression and / or activity of a dual-target gene. The method comprises: providing a complex or a nucleic acid encoding the complex, wherein the complex comprises a DNA binding domain and a gene expression modulator. For example, the method simultaneously regulates the expression and / or activity of PCSK9 gene and ANGPTL3 gene. For example, the method simultaneously regulates the expression and / or activity of APOC3 gene and the ANGPTL3 gene. For example, the method simultaneously regulates the expression and / or activity of the PCSK9 gene and the APOC3 gene.

The invention discloses a polyethyleneimine low-toxicity nano-carrier capable of being used for delivering DNA (deoxyribonucleic acid) molecules as well as a preparation method and application of the polyethyleneimine low-toxicity nano-carrier, and the polyethyleneimine low-toxicity nano-carrier can be used for accurately controlling surface charges and particle sizes of PEI nano-particles by adjusting parameters such as molecular weight, N / P ratio and the like of PEI, so that an ideal delivery effect is achieved, components and structures of a compound are reasonably designed, and gene delivery performance is optimized; a safe, efficient and reusable DNA delivery solution is provided for the fields of scientific research and medical treatment, and the method is of great significance in promoting gene therapy, vaccine development and other leading-edge scientific and technological progress.

The invention provides screening and analysis of LncRNA related to skeletal muscle development in the muscle development process of Tan sheep in a salt pond, and particularly relates to application of Lnc.23721 and a target gene CDKN1A thereof in regulation and control of skeletal muscle development of Tan sheep, and the sequence of the CDKN1A gene is as shown in SEQ ID NO.1. The invention further provides application of the Lnc.23721 and the target gene CDKN1A in regulation and control of skeletal muscle development of Tan sheep in the salt pond Tan sheep. Specifically, the expression level of the CDKN1A is up-regulated, the differentiation of the skeletal muscle cells and myotube formation of the Tan sheep can be inhibited, the expression level of the CDKN1A is down-regulated, and the myogenic differentiation and slow muscle fiber formation of the skeletal muscle cells of the Tan sheep can be promoted, so that the meat production performance of the Tan sheep can be improved, and the formation of tender meat is facilitated. The invention also provides the LncRNA capable of regulating and controlling the development of the skeletal muscle of the Tan sheep, and the nucleotide sequence of the LncRNA Lnc.23721 is as shown in SEQ ID NO. 2. Specifically, by down-regulating the expression level of Lnc.23721, the differentiation of the Tan sheep skeletal muscle cells can be inhibited.

The present disclosure provides drug-responsive domains derived from human carbonic anhydrase 2 that can modulate the protein stability of human interleukin-15 (IL15) payloads, as well as compositions and methods of use thereof.

Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and / or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

The invention discloses a target segment and double-stranded RNA for preventing and controlling propagation of tomato brown crinkled fruit virus through tomato seeds and application of the target segment and the double-stranded RNA. Wherein the nucleotide sequence of the target segment is as shown in SEQ ID NO.3, or a sequence which is as shown in SEQ ID NO.3, has at least 90% of homology and has coded amino acid as shown in SEQ ID NO.4 is substituted. The invention further discloses double-stranded RNA of the target interference target section, the double-stranded RNA is used for single isolation treatment or batch treatment of germinated tomato seeds, and the infection rate of the tomato brown crinkled fruit virus on germinated tomato seedlings can be effectively reduced under the condition that the germination rate of the tomato seeds is not affected. The method for preventing and controlling propagation of the tomato brown crinkled fruit virus through the tomato seeds by using the double-stranded RNA is safe and harmless, and has a good application prospect.

Disclosed are conjugates of an oligonucleotide and a peptide covalently bonded or linked via a linker to the oligonucleotide, the peptide including at least one cationic domain comprising at least 4 amino acid residues and at least one hydrophobic domain comprising at least 3 amino acid residues, provided that the peptide includes a total of 7 to 40 amino acid residues and does not include any artificial amino acid residues; and the oligonucleotide including a total of 12 to 40 contiguous nucleobases, where at least 12 contiguous nucleobases are complementary to a target sequence in a human dystrophin gene.

The invention relates to the technical field of biological medicine. The invention provides highly branched poly (beta-amino ester) and a preparation method thereof. The invention also provides lipid nanoparticles which contain the compound and are used for efficiently delivering mRNA, a preparation method of the lipid nanoparticles, and application of the lipid nanoparticles in preparation of CD5 antibody targeted nucleic acid drug delivery carriers. The highly branched poly (beta-amino ester) prepared by the scheme of the invention has better mRNA binding capacity and in-vivo stability, the obtained lipid nanoparticles have high transfection efficiency, and efficient transfection of specific tissues or cells can be better realized.

Disclosed are a protein TaNHX2 related to wheat drought stress, and a gene encoding same and the use thereof. The technical problem to be solved is how to improve the drought resistance of Angiospermae plants. Specifically, disclosed is the use of a protein as shown in sequence 2 or 4, a substance that up-regulates or enhances or improves the expression of the gene encoding the protein, or a substance that up-regulates or enhances or improves the activity or content of the protein, which use is characterized in that the use is any one of: A1) use in the improvement of the drought resistance of Angiospermae plants; and A2) use in the preparation of a product for improving the drought resistance of Angiospermae plants. Improving the expression of the protein in Angiospermae plants can improve the drought resistance of the plants. The present invention can be applied to agricultural production.

the present invention relates to peptides, in particular cell-penetrating peptides, having a first hydrophobic domain positioned at the C-terminus of the peptide and a second hydrophobic domain positioned at the N-terminus of the peptide, and to conjugates of such cell-penetrating peptides with a therapeutic molecule. The present invention further relates to use of such peptides or conjugates in methods of treatment or as a medicament, especially in the treatment of genetic disorders and in particular muscular dystrophies such as Duchenne muscular dystrophy.

Described herein are compositions and methods for modifying eukaryotic cells, for example, to express a transgene of interest and / or to produce an expanded population of cells ex vivo. Using the compositions and methods of the disclosure, a population of eukaryotic cells, such as a population of pluripotent cells (e.g., CD34+ hematopoietic stem or progenitor cells) may be transduced to express a gene of interest by contacting the cells with a viral vector, such as a lentiviral vector, and a diblock copolymer, such as a diblock copolymer composed of a hydrophilic region and a hydrophobic region. For example, the diblock copolymer may be composed of polyoxyethylene (PEO) subunits and polyoxypropylene (PRO) subunits. Additionally, the compositions and methods described herein can be used to promote the proliferation or survival of a population of pluripotent cells (e.g., CD34+ hematopoietic stem or progenitor cells) ex vivo, for example, by contacting the cells with a diblock copolymer.