31 results about "Myogenic differentiation" patented technology

The invention relates to the field of biomedicine and particularly relates to oligonucleotide for promoting repair of skeletal muscle injury and an application method thereof. The invention firstly discloses oligonucleotide which is screened from transcripts of human-derived growth imprinted gene H19 and has a function of obviously promoting myogenic differentiation of skeletal-muscle myoblast. The oligonucleotide is characterized by being shown in sequences of SEQ ID NO:1 and SEQ ID NO:2. The oligonucleotide and the application method have the advantages of simple operation, no risk, promotion for fast regeneration of new muscle fiber after skeletal muscle injury and good function restoration. Therefore, the oligonucleotide and the application method have good application prospect in genetherapy of skeletal muscle injury caused by acute contusion, crush injury, explosion injury or chemical substances.

The present invention provides cells transformed with a nucleotide sequence encoding Pax7, Pax3 or both. The present invention also pertains to Pax-encoding vector that comprises a sequence encoding Pax7, Pax3 or an active variant or fragment thereof, which can be used to induce myogenic differentiation of stem cells. The present invention further pertains to methods of preparing the Pax-encoding vector. Also provided is a method of inducing myogenic differentiation in stem cells and treating a subject with the cells.

The invention relates to a method for promoting myogenic differentiation of bovine skeletal muscle satellite cells. The method is characterized in that according to the method, the myogenic differentiation of bovine skeletal muscle satellite cells is promoted by increasing the expression of lncRNA-23, wherein the genome base sequence of the lncRNA-23 is SEQ NO.1. The method can provide inspirationfor researching long non-coding RNA of muscle development differentiation, can lay a good foundation for further researching the action mechanism of key lncRNA in the muscle development process, andprovides inspiration for exploring the muscle growth and development mechanism and improving the yield and quality of beef.

Disclosed are methods of differentiating stem cells into muscle cells by growing the cells in a myogenic culture medium. The differentiated cells can be used as a source of cells for transplantation in a patient in need thereof.

The present invention relates to a method for promoting the myogenic differentiation of bovine skeletal muscle satellite cells by overexpressing PSMB5, ​​the steps are as follows: (1) obtaining the target sequence comprising the bovine PSMB5 gene sequence; (2) constructing the expression vector pcDNA3.1-PSMB5; (3) pcDNA3.1 Transfect bovine skeletal muscle satellite cells with ‑PSMB5: use the obtained expression vector pcDNA3.1‑PSMB5, ​​and use liposome transfection reagent to transfect bovine skeletal muscle satellite cells, so that bovine skeletal muscle satellite cells overexpress PSMB5, ​​and utilize the effect of PSMB5 , to improve the in vitro differentiation capacity of bovine skeletal muscle satellite cells. The method of the present invention uses PSMB5 to regulate the proliferation and myogenic differentiation of bovine muscle satellite cells, which can provide enlightenment for the study of the ubiquitination process of muscle development and differentiation, and provide new insights for clinical research and diagnosis and treatment of muscle development and differentiation and injury repair. ideas and references.

The invention relates to a method for inhibiting the myogenic differentiation of bovine skeletal muscle satellite cells by interfering with the expression of UCH-L3, wherein the method is to suppress the myogenic differentiation of bovine skeletal muscle satellite cells by interfering with the expression of UCH-L3, wherein the The genome base sequence of UCH‑L3 is: SEQ NO.1. The method of the present invention regulates the myogenic differentiation process of bovine muscle satellite cells by changing the expression of UCH-L3, which can provide enlightenment for the research on deubiquitinating enzymes of muscle development and differentiation, and provide clinical research, diagnosis and treatment for muscle development and differentiation and injury repair Provide new ideas and references.

The invention relates to a method for inhibiting myogenic differentiation of bovine skeletal muscle satellite cells by interfering UCH-L3 expression, the myogenic differentiation of bovine skeletal muscle satellite cells is inhibited by interfering UCH-L3 expression, and the genome base sequence of UCH-L3 is SEQ NO.1. The invention also relates to a kit for inhibiting myogenic differentiation of bovine skeletal muscle satellite cells by interfering UCH-L3 expression. According to the method, the myogenic differentiation process of bovine muscle satellite cells is regulated and controlled by changing UCH-L3 expression, enlightenment can be provided for deubiquitination enzyme research of muscle development and differentiation, and new thought and reference are provided for clinical researchand diagnosis and treatment of muscle development and differentiation and injury repair.

The invention relates to the field of biological medicine, in particular to a CD24 positive expression cell in urine and a preparation method and application thereof. According to the application, cells with CD24 positive expression in urine-derived cells are found and screened from urine of a muscle injury patient, and the cells with CD24 positive expression in the urine cells can be subjected to myogenic differentiation under an in-vitro induction condition and are fused with muscle cells to form fused muscular tubules; it is verified that after CD24 positive expression cells in urine cells are transplanted into animal bodies, inflammatory reaction symptoms can be relieved at muscle injury parts, muscle regeneration and repair can be achieved, the CD24 positive expression cells can be used for preparing related medicine for repairing muscle injury treatment, the cells for treatment are derived from patient bodies and have the characteristic of individualized precise treatment, and the CD24 positive expression cells can be used for preparing the related medicine for repairing muscle injury treatment. The autologous cell treatment of the patient can be quickly realized, and the immunological rejection of transplantation is avoided.

The invention relates to a method for promoting myogenic differentiation of bovine skeletal muscle satellite cells. According to the method, myogenic differentiation of bovine skeletal muscle satellite cells is promoted by interfering lncRNA-HZ5 expression. The specific method comprises the steps as follows: siRNA of lncRNA-HZ5 is designed and synthesized according to an lncRNA-HZ5 base sequence, siRNA is used for transfection of the bovine skeletal muscle satellite cells, the expression level of lncRNA-HZ5 is lowered, and myogenic differentiation of the bovine skeletal muscle satellite cells is promoted, wherein the genome base sequence of lncRNA-HZ5 is shown in SQ1. The myogenic differentiation process of the bovine skeletal muscle satellite cells regulated by changing lncRNA-HZ5, enlightenment is provided for long non-coding RNA research of muscle development differentiation, and a new idea and reference are provided for clinical research, diagnosis and treatment of muscle development differentiation and damage repair.

The invention discloses the application of a non-coding small RNA miR-192 in inhibiting the myogenic differentiation of sheep skeletal muscle satellite cells and promoting the proliferation of sheep skeletal muscle satellite cells. The analog of the non-coding small RNA miR-192 is transfected into sheep The overexpression of intracellular miR‑192 in skeletal muscle satellite cells, compared with the cells overexpressing the control nucleotide sequence, the differentiation of sheep skeletal muscle satellite cells was inhibited, but the proliferation of sheep skeletal muscle satellite cells was inhibited. Capabilities are improved. The present invention also provides the application of miR-192 to inhibit the expression of RB1 protein level in sheep skeletal muscle satellite cells. The present invention discovers for the first time that miR-192 has the function of negatively regulating the myogenic differentiation of sheep skeletal muscle satellite cells and promoting the proliferation of sheep skeletal muscle satellite cells.

The invention discloses application of a short peptide MPM in the preparation of a medicine for treating diseases related to muscle cell differentiation. An amino acid sequence of the short peptide MPM is shown in SEQ ID NO. 1 or SEQ ID NO. 2, a nucleotide sequence of LINC00116 encoding the short peptide MPM is shown in SEQ ID NO. 3 or SEQ ID NO. 4. The application of the short peptide MPM in thepreparation of the medicine for treating the diseases related to the muscle cell differentiation finds that the short peptide MPM plays a key role in cell myogenic differentiation, skeletal muscle development and regeneration, muscle fiber development and regeneration; high MPM expression promotes cell myogenic differentiation, while low expression inhibits the myogenic differentiation of cells, and the expression level of MPM directly affects the maximum grip and mouse exercise tolerance. Compared with normal people, the expression level of the MPM is decreased in the diseased muscles of patients with tibial muscular dystrophy (DMD), Duchenne muscular dystrophy (TMD), rhabdomyosarcoma of different pathological types and the muscles of the elderly; and it indicates that the short peptide MPM is associated with muscle cell differentiation, the differentiation of the muscle cells can be promoted by promoting the expression of the MMP, and the medicine for treating diseases related to themuscle cell differentiation is prepared.

The invention discloses a method for inducing myogenic differentiation of adipose stem cells (ADSCs), the method comprising placing the adipose stem cells (ADSCs) in a 2 In a culture environment, induce 3-9 days at a temperature of 35-40°C. The present invention found that after 9 days of induction, the cells expressed troponin, myosin, MyoD and MHC, and at the same time, the expression of sarcomere α-actin and the differentiation rate of myoblasts were significantly higher than those of the control group. The present invention uses hydrogen to effectively promote the myogenic differentiation of ADSCs for the first time. Compared with 5-azacytidine (5-Aza) administration, hydrogen exposure is a simpler, non-toxic and cheap differentiation method. At the same time, Hydrogen also increased the effect of 5‑Aza on ADSC myoblast differentiation. It can be seen that hydrogen is the recommended inducer to realize the differentiation of MSCs into muscle fibers, and has broad application prospects.

The invention discloses a cultivation method for improving the oxidative metabolism ability of chicken skeletal muscle cells, which belongs to the field of biotechnology. The method provided by the present invention is to inoculate the separated and purified chicken skeletal muscle myoblasts and myogenic fibroblasts in the upper chamber and the lower chamber of the Transwell culture dish respectively, and the two are separated by a PET membrane with a pore size of 1 μm but shared. There is H 2 o 2 myogenic differentiation medium; Myotubes with voluntary contraction ability were obtained after myogenic differentiation induction culture for 7-10 days, during which low-temperature shock stimulation was performed every 8-12 hours. The method provided by the invention can significantly increase the mitochondrial content of chicken skeletal muscle cells, the transmembrane potential and the activity of aerobic metabolism enzymes, while reducing the activity of ATPase. This method has the characteristics of short cultivation period and stable effect, and it has laid a foundation for the study of chicken skeletal muscle energy metabolism, fiber type formation mechanism, etc., and has good research and application value.