26 results about "Myogenic differentiation" patented technology

The invention provides a method for producing cell culture meat through 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres, and belongs to the technical field of preparation of cell culture meat. A method for producing cell culture meat by 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres comprises the following steps: mixing and inoculating pseudosciaena crocea myoblasts and pseudosciaena crocea precursor adipocytes into a cell culture plate subjected to anti-adhesion treatment for proliferation culture, spontaneously gathering to form cell spheres, and carrying out differentiation culture to obtain the cell culture meat. According to the co-culture mode provided by the invention, high-efficiency myogenic differentiation and adipogenic differentiation of myoblasts and precursor adipocytes of the large yellow croakers can be realized through proliferation culture and differentiation culture, and an in-vivo microenvironment can be effectively simulated, so that cultured meat similar to a traditional meat product in texture and flavor is produced.

The invention provides screening and analysis of LncRNA related to skeletal muscle development in the muscle development process of Tan sheep in a salt pond, and particularly relates to application of Lnc.23721 and a target gene CDKN1A thereof in regulation and control of skeletal muscle development of Tan sheep, and the sequence of the CDKN1A gene is as shown in SEQ ID NO.1. The invention further provides application of the Lnc.23721 and the target gene CDKN1A in regulation and control of skeletal muscle development of Tan sheep in the salt pond Tan sheep. Specifically, the expression level of the CDKN1A is up-regulated, the differentiation of the skeletal muscle cells and myotube formation of the Tan sheep can be inhibited, the expression level of the CDKN1A is down-regulated, and the myogenic differentiation and slow muscle fiber formation of the skeletal muscle cells of the Tan sheep can be promoted, so that the meat production performance of the Tan sheep can be improved, and the formation of tender meat is facilitated. The invention also provides the LncRNA capable of regulating and controlling the development of the skeletal muscle of the Tan sheep, and the nucleotide sequence of the LncRNA Lnc.23721 is as shown in SEQ ID NO. 2. Specifically, by down-regulating the expression level of Lnc.23721, the differentiation of the Tan sheep skeletal muscle cells can be inhibited.

The invention provides a fish precursor fat cell myogenic differentiation and pluripotent differentiation culture medium, a method for inducing myogenic differentiation and pluripotent differentiation and application, and particularly belongs to the technical field of cell biology. The differentiation culture medium for inducing the fish precursor fat cells to differentiate into the muscle cells comprises a myogenic differentiation culture medium and a differentiation maintenance culture medium. According to the myogenic differentiation culture medium, the large yellow croaker precursor fat cells can be induced to be differentiated towards muscle cells through the regulation and control effect of growth factors, and the differentiation maintaining culture medium can maintain myogenic differentiation and enhance the quality of myotubes; by combining the adipogenic differentiation culture medium, the directional pluripotent differentiation of the precursor adipocytes to different types of cells can be finally realized, and myotubes and adipocytes are efficiently formed. The preparation of the cell culture meat provides a new strategy and method.

The invention discloses the technical field of animal cell culture and cell culture meat, and provides a food-grade natural compound combination method for promoting efficient myogenic differentiation in vitro. According to the method disclosed by the invention, a combination of xanthophyll and arachidic acid is added into a basic myogenic differentiation induction culture medium to form a myogenic differentiation promoting culture medium. According to the myogenic differentiation culture medium system, the myogenic differentiation efficiency of the myogenic cells can be improved from about 23% to 45% or above, the myogenic fusion efficiency can be improved from about 18% to 33% or above, the problems that the in-vitro differentiation efficiency of the myogenic cells is low, the content of key proteins such as myosin is insufficient and the like are effectively solved, and the myogenic differentiation culture medium system is suitable for large-scale popularization and application. And a feasible scheme is provided for large-scale muscle fiber production of cell culture meat.

The invention provides a food capable of improving muscle quality and functions as well as a preparation method and application of the food. The food contains lactoferrin and creatine, and the mass ratio of the lactoferrin to the creatine is (3-9): 1. In terms of the total dry matter content of the food, the content of the lactoferrin in the food is 0.1-90 g / 100 g, and the content of the creatine in the food is 0.01-30 g / 100 g. The food disclosed by the invention can play an important role in preventing, treating and improving the sarcopenia syndrome by improving muscle quality, muscle strength and functions, promoting myogenic differentiation and the like in multiple aspects and multiple dimensions.

Provided is a method for inducing muscle-derived differentiation of porcine pluripotent stem cells, which does not involve transgenosis and is free of serum addition in the whole course, and also provided is a method for preparing cell cultured meat (CM) based on the muscle-derived differentiation induction method.

The invention relates to the technical field of biological breeding, in particular to application of BMP6 protein or a coding gene thereof to regulation and control of myogenic differentiation of poultry skeletal muscle satellite cells. The invention finds that the BMP6 gene is a key factor for regulating and controlling the senescence of the muscle-derived satellite cells and inducing the cells to be converted from an activated state to a resting state through the research of an snRNA-seq combined in-vivo and in-vitro method, and proves that the knock-down of the BMP6 can promote the activation and myogenic differentiation of the skeletal muscle satellite cells. The invention provides a new way for improving the meat production performance of poultry, breeding an improved variety and preventing and treating related myopathy.

This invention relates to the application of *Hirudo medicinalis* in the preparation of drugs that promote skeletal muscle cell differentiation and / or prevent muscle atrophy, belonging to the field of biomedical technology. The skeletal muscle cells are myoblasts. In vitro, this drug can promote differentiation by upregulating the expression of myosin heavy chain (MyHC), myogenin, and myogenic determinant MyoD, thereby increasing myotube diameter. In vivo, it can improve doxorubicin-induced skeletal muscle atrophy in mice and repair muscle fiber damage caused by natural aging nematodes. The chemotherapeutic drug is doxorubicin, which can exert an anti-atrophy effect by maintaining the expression of myosin heavy chain (MyHC) and improving myotube morphology damage. This invention is the first to discover and experimentally demonstrate that *Hirudo medicinalis* freeze-dried powder has the activity of promoting myoblast differentiation and preventing muscle atrophy, opening up new applications for traditional Chinese medicine leeches in the prevention and treatment of muscle atrophy and expanding the medicinal value of *Hirudo medicinalis*.

This invention discloses a multifunctional injectable hyaluronic acid hydrogel, its preparation method, and its applications. The hydrogel comprises: a methacrylamide hyaluronic acid hydrogel matrix; cobalt-containing composite microspheres dispersed in the matrix, wherein the cobalt-containing composite microspheres are gelatin methacrylate / alginate methacrylate microspheres loaded with citric acid-coated cobalt nanoparticles; and miR-206-3p-enriched exosomes loaded in the hydrogel. The multifunctional injectable hyaluronic acid hydrogel provided by this invention organically integrates the hyaluronic acid matrix, cobalt-containing functional microspheres, and miR-206-3p-enriched exosomes, and can simultaneously provide multiple functions such as structural support, local gelation, angiogenesis promotion, and myogenic differentiation promotion. It overcomes the shortcomings of single materials in meeting the comprehensive repair needs of acute skeletal muscle injury, and provides a comprehensive repair strategy for acute skeletal muscle injury that combines structural support, angiogenesis induction, myogenic differentiation regulation, and anti-fibrosis functions.

The invention provides a food for improving muscle attenuation and promoting myogenic differentiation as well as a preparation method and application of the food. The food comprises lactoferrin and coenzyme Q10, wherein the mass ratio of the lactoferrin to the coenzyme Q10 is (40-60): 1; based on the total dry matter content of the food, the content of the lactoferrin in the food is 0.1 to 98g / 100g, and the content of the coenzyme Q10 in the food is 1.7 to 2450mg / 100g. The food disclosed by the invention can play an important role in preventing, treating and improving the sarcopenia syndrome by improving muscle quality, muscle strength and functions, promoting myogenic differentiation and the like in multiple aspects and multiple dimensions.

The present invention relates to a method for producing a composition which comprises animal protein, comprising (a) isolating precursor cells from perinatal tissue of a mammal; (b) incubating the precursor cells under conditions which lead to a myogenic differentiation of the precursor cells; and (c) harvesting the cells. The present invention also relates to a method for producing precursor cells from perinatal tissue, to animal protein produced according to the invention, and precursor cells produced according to the invention. The present invention further relates to the use of a culture medium which has a reduced content of methionine in comparison to standard medium, to the differentiation of precursor cells, and to a method for the in vitro production of a meat-like composition.

The invention discloses an application of mouse muscle cell-derived apoptosis vesicles in muscle physiological regulation, the vesicles are apoptosis vesicles generated in a myogenic differentiation process, are derived from a normal physiological state and are not generated by chemical drug induction, and vesicle content abnormity caused by damage of a cell homeostasis under drug stimulation is avoided; experimental data shows. The apoptosis vesicles can promote differentiation and development of skeletal muscle cells in vivo and in vitro, remarkably delay muscle atrophy caused by aging or diabetes, and contribute to research and development of skeletal muscle disease related drugs.

This invention discloses the use of FNDC1 protein to promote skeletal muscle regeneration and treat muscle development-related diseases. Researchers found that when C2C12 cells were treated with FNDC1 protein, the ability of myotubes to fusion increased with increasing concentrations of the recombinant FNDC1 protein, indicating that FNDC1 protein can promote myogenic differentiation. Furthermore, treatment of CTX mice with FNDC1 protein revealed that it promoted muscle regeneration. This suggests that FNDC1 protein can be used to prepare drugs and feeds for treating skeletal muscle dysplasia.

This invention belongs to the field of cultured meat and relates to a method for constructing a cultured meat scaffold. The invention involves preheating a deionized aqueous solution of soybean protein and then cooling it to room temperature to obtain a soybean protein solution; dissolving fish gelatin in deionized water to obtain a fish gelatin solution; mixing the soybean protein solution and the fish gelatin solution, stirring until homogeneous to obtain a mixed solution; then adding glutaminase solution; heating and incubating to inactivate the enzyme; and after full hydration, obtaining a gel scaffold, which is then ready for consumption as a dual-protein cultured meat scaffold. Preheating and enzymatic cross-linking are used to regulate the dual-protein gel network, reducing its heterogeneity and improving its stability and strength. The dual-protein gel scaffold possesses excellent mechanical properties, a uniform network structure, and biocompatibility, which can improve the adhesion, proliferation, and spreading area of ​​porcine skeletal muscle satellite cells, while simultaneously meeting the requirements for myoblastic differentiation.

The invention belongs to the technical field of biology and cells, and discloses application of a circAAGAB gene in regulation and control of proliferation and differentiation of bovine skeletal muscle satellite cells, the circAAGAB gene truly exists and is in a ring shape, and the nucleotide sequence of the circAAGAB gene is shown as SEQ ID NO.1. The circAAGAB gene has a nucleotide sequence shown as SEQ ID NO.2. The circAAGAB gene has a nucleotide sequence shown as SEQ ID NO.1. According to the method disclosed by the invention, the proliferation and myogenic differentiation process of the bovine muscle satellite cells can be effectively regulated and controlled by regulating the expression of circAAGAB. A new enlightenment is provided for researching muscle development and differentiation, and an important reference is provided for exploring a muscle growth and development mechanism and improving the yield and quality of beef. In addition, a good foundation is laid for deeply discussing the action mechanism of circAAGAB in the muscle development process, and theoretical support is provided for rapid breeding and meat quality improvement of new varieties of high-quality beef cattle.

The invention discloses a bovine ectoderm stem cell line as well as an establishment method and application thereof. The bovine ectoderm stem cell line has the pluripotency of a bovine intermediate state stem cell line, expresses one or more pluripotency markers, can be stably passaged, still maintains pluripotency transcriptome characteristics and normal karyotype similar to those of an intermediate state (Formative) ectoderm cell after more than 112 generations, and has the potential of being differentiated into three-germ layers. The bovine intermediate-state stem cell line has the potential for myogenic differentiation, primordial germ cell-like cell differentiation and serving as somatic cell nuclear transplantation (SCNT) donor cells, which shows that the bovine intermediate-state stem cell line has wide application prospects in the aspects of promoting cell culture meat production and animal breeding.

The invention discloses a serum-free culture medium and application thereof in culture of large yellow croaker muscle stem cells. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, wherein the exogenous additive components comprise HEPES, sodium chloride, hydroxypropyl-beta-cyclodextrin, sodium metavanadate, vitamin C magnesium phosphate, omega-3 polyunsaturated fatty acid, pyridoxine hydrochloride, nicotinamide, cholesterol, calcium chloride, potassium chloride, a soybean pancreatin inhibitor, taurine, trehalose, ethanolamine, a telomerase activator and recombinant human serum albumin. The animal-derived protein-free serum-free culture medium disclosed by the invention does not contain animal-derived components such as serum, does not need to add components such as growth factors or hormones, not only has definite chemical components, high safety, low cost and high cell proliferation speed, but also can effectively improve the myogenic differentiation potential of muscle stem cells and effectively activate telomerase activity to solve the problem of cell aging; and a feasible product and a feasible method are provided for culture of fish cells and large-scale production of cell culture meat.

The invention relates to application of a porcine TEAD4 gene in regulating and controlling myogenic differentiation of porcine skeletal muscle satellite cells. The invention finds that the mRNA level and the TEAD4 protein level of the pig TEAD4 gene are continuously, extremely and remarkably up-regulated in the myogenic differentiation process of the pig skeletal muscle satellite cells, and the myogenic differentiation of the pig skeletal muscle satellite cells can be effectively inhibited by inhibiting the expression of the TEAD4 gene. On the basis, the myogenic differentiation of the pig skeletal muscle satellite cells is regulated and controlled by regulating and controlling the expression level of the TEAD4 gene, the meat production traits of pigs can be regulated and controlled, and the development of animal husbandry is promoted.

The invention provides application of a small-molecule inhibitor in promoting proliferation and / or muscle-derived differentiation of pseudosciaena crocea satellite cells, and belongs to the technical field of cell culture. The invention relates to application of a small-molecule inhibitor in promoting proliferation and / or muscle-derived differentiation of large yellow croaker satellite cells. The small-molecule inhibitor comprises at least one of the following components: MHY1485, SB431542 and CHIR99021. Experiments show that the three small-molecule inhibitors can effectively promote proliferation of large yellow croaker satellite cells, accelerate the cell cycle progress and improve the muscle source differentiation potential at the same time. According to the technical scheme, a new theoretical basis and a new practical method are provided for efficient culture of the large yellow croaker satellite cells, commercial production of cell culture meat is promoted, and a new path is provided for sustainable meat production.

The invention relates to the technical field of biomedical engineering, and provides a culture system for reprogramming pluripotent stem cell-derived neural crest cells into muscle progenitor cells, an induction method and application, the system is composed of three differential culture media, the first differential culture medium is used for inducing and differentiating pluripotent stem cells into the neural crest cells, and the second differential culture medium is used for inducing and differentiating the pluripotent stem cells into the muscle progenitor cells; the neural crest cells are induced and differentiated into muscle progenitor cells by the second differentiation medium, and the muscle progenitor cells are subjected to myogenic differentiation verification by the third differentiation medium. According to the method, the muscle progenitor cells capable of realizing long-term stable self-renewal can be obtained by differentiating the neural crest cells derived from the human pluripotent stem cells. The muscle progenitor cell can be stably amplified for more than 30 generations in a culture system established by the invention, has rapid and efficient myogenic differentiation capacity, can be differentiated in vivo to form thick muscle bundles, and can be colonized for a long time in an acute muscle injury mouse model and participate in injury repair. The muscle progenitor cell is expected to be developed into a novel cell source for treating muscle injury related diseases.

The application discloses a kind of biological absorbable double-layer heterogeneous membranes for promoting tendon-bone interface healing, preparation method and application, belong to the field of biomedical materials.Using solution casting method, freeze-drying technology and photoetching technology, a kind of absorbable double-layer membrane with asymmetric structure is prepared by taking natural polymer material chitosan and gelatin as main raw materials.The groove orientation side of the membrane wraps tendon / ligament graft, which can effectively arrange and lengthen fibroblasts at the interface, promote cell myogenic differentiation and reduce scar hyperplasia tissue generation caused by disordered arrangement;The porous sponge side is attached to the bone tunnel, which can improve cell adhesion and proliferation capacity, and promote bone formation at the interface.The material has good flexibility, can provide certain mechanical properties for the affected area, can be degraded in vivo, and the product has no toxic and side effects on the body.The two structures jointly promote tendon-bone interface healing and reduce adverse reactions.The material has no external drug stimulation, good biological safety, low price, simple preparation process, strong operability, good adjustability for different affected areas, strong clinical execution ability, and has wide application prospect.

The invention belongs to the technical field of biology, cell and tissue engineering, and discloses application of an FGF16 gene in regulation and control of bovine skeletal muscle satellite cell differentiation, and the nucleotide sequence of the FGF16 gene is SEQ ID NO.1. According to the method disclosed by the invention, the proliferation and myogenic differentiation process of the bovine muscle satellite cells can be effectively regulated and controlled by regulating the expression of the FGF16. A new enlightenment is provided for researching muscle development and differentiation, and an important reference is provided for exploring a muscle growth and development mechanism and improving the yield and quality of beef. In addition, a good foundation is laid for deeply discussing the action mechanism of the key FGF16 protein in the muscle development process, and theoretical support is provided for rapid breeding and meat quality improvement of new varieties of high-quality beef cattle.

The invention belongs to the field of preparation of medical materials, and particularly relates to application of acellular matrix loaded Sr-Se coated SiO2 and adipose-derived mesenchymal stem cells in sequential treatment of volumetric muscle defects. On the basis of the Sr-Se-coated SiO2 nano composite material, a composite filling scaffold is developed in combination with a tissue engineering scaffold, and the effect of the composite filling scaffold in the VML treatment process is researched. The filler is composed of three parts, namely a nano material Sr-Se coated SiO2 with multiple functions of resisting inflammatory response, promoting vascularization and the like, ADSCs with myogenic differentiation potential and a dECM composite scaffold used for loading contents, the composite filling scaffold provided by the invention can improve the problem that traditional VML treatment is difficult, and sequential treatment of personalized guide VML is achieved.

This invention relates to the field of tissue engineering technology and discloses a multi-level oriented muscle repair scaffold made from chitin nanocrystals and its preparation method. The invention uses chitin derived from shrimp and crab shells as raw material to prepare chitin nanocrystals. Through steps such as preparing an aqueous suspension of chitin nanocrystals, ultrasonic dispersion, directional freezing to induce ice crystal orientation growth, freeze-drying to control ice crystal sublimation, and glutaraldehyde cross-linking fixation, a multi-level oriented scaffold with a macroscopic oriented pore structure and a microscopic oriented micro / nanofiber structure is prepared, with the chitin nanocrystals arranged along the orientation pore direction. This scaffold has a high specific surface area, can promote the orientation alignment and myogenic differentiation of C2C12 cells, and can be effectively used for the repair of volumetric muscle tissue loss. Furthermore, the preparation method is simple, requires low-end equipment, is inexpensive, and can be mass-produced.

This invention relates to the fields of improving the immune microenvironment, promoting angiogenesis and bone regeneration, and specifically to a multifunctional nanomaterial for rotator cuff injury complicated with osteoporosis and its preparation method. This invention develops a multifunctional nanomaterial, Nb2C@Mg-MOF (denoted as NM), for rotator cuff injury complicated with osteoporosis. This nanomaterial exhibits good biocompatibility, can scavenge reactive oxygen species, effectively inhibits inflammatory responses, adipogenic differentiation and osteoclastogenesis, promotes angiogenesis, osteogenic and myogenic differentiation, and can effectively inhibit the progression of osteoporosis and fatty infiltration, while promoting rotator cuff tendon-bone healing.

A culture medium and a culture medium additive which can promote myogenic differentiation and which contain ergothioneine, ascorbic acid 2-glucoside, ascorbic acid, or a combination thereof. A kit, pharmaceutical composition, food, and drink may also promote myogenic differentiation.