14 results about "Myogenic differentiation" patented technology

The invention provides a method for producing cell culture meat through 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres, and belongs to the technical field of preparation of cell culture meat. A method for producing cell culture meat by 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres comprises the following steps: mixing and inoculating pseudosciaena crocea myoblasts and pseudosciaena crocea precursor adipocytes into a cell culture plate subjected to anti-adhesion treatment for proliferation culture, spontaneously gathering to form cell spheres, and carrying out differentiation culture to obtain the cell culture meat. According to the co-culture mode provided by the invention, high-efficiency myogenic differentiation and adipogenic differentiation of myoblasts and precursor adipocytes of the large yellow croakers can be realized through proliferation culture and differentiation culture, and an in-vivo microenvironment can be effectively simulated, so that cultured meat similar to a traditional meat product in texture and flavor is produced.

The invention provides a fish precursor fat cell myogenic differentiation and pluripotent differentiation culture medium, a method for inducing myogenic differentiation and pluripotent differentiation and application, and particularly belongs to the technical field of cell biology. The differentiation culture medium for inducing the fish precursor fat cells to differentiate into the muscle cells comprises a myogenic differentiation culture medium and a differentiation maintenance culture medium. According to the myogenic differentiation culture medium, the large yellow croaker precursor fat cells can be induced to be differentiated towards muscle cells through the regulation and control effect of growth factors, and the differentiation maintaining culture medium can maintain myogenic differentiation and enhance the quality of myotubes; by combining the adipogenic differentiation culture medium, the directional pluripotent differentiation of the precursor adipocytes to different types of cells can be finally realized, and myotubes and adipocytes are efficiently formed. The preparation of the cell culture meat provides a new strategy and method.

The invention discloses the technical field of animal cell culture and cell culture meat, and provides a food-grade natural compound combination method for promoting efficient myogenic differentiation in vitro. According to the method disclosed by the invention, a combination of xanthophyll and arachidic acid is added into a basic myogenic differentiation induction culture medium to form a myogenic differentiation promoting culture medium. According to the myogenic differentiation culture medium system, the myogenic differentiation efficiency of the myogenic cells can be improved from about 23% to 45% or above, the myogenic fusion efficiency can be improved from about 18% to 33% or above, the problems that the in-vitro differentiation efficiency of the myogenic cells is low, the content of key proteins such as myosin is insufficient and the like are effectively solved, and the myogenic differentiation culture medium system is suitable for large-scale popularization and application. And a feasible scheme is provided for large-scale muscle fiber production of cell culture meat.

Provided is a method for inducing muscle-derived differentiation of porcine pluripotent stem cells, which does not involve transgenosis and is free of serum addition in the whole course, and also provided is a method for preparing cell cultured meat (CM) based on the muscle-derived differentiation induction method.

The invention relates to the technical field of biological breeding, in particular to application of BMP6 protein or a coding gene thereof to regulation and control of myogenic differentiation of poultry skeletal muscle satellite cells. The invention finds that the BMP6 gene is a key factor for regulating and controlling the senescence of the muscle-derived satellite cells and inducing the cells to be converted from an activated state to a resting state through the research of an snRNA-seq combined in-vivo and in-vitro method, and proves that the knock-down of the BMP6 can promote the activation and myogenic differentiation of the skeletal muscle satellite cells. The invention provides a new way for improving the meat production performance of poultry, breeding an improved variety and preventing and treating related myopathy.

This invention relates to the application of *Hirudo medicinalis* in the preparation of drugs that promote skeletal muscle cell differentiation and / or prevent muscle atrophy, belonging to the field of biomedical technology. The skeletal muscle cells are myoblasts. In vitro, this drug can promote differentiation by upregulating the expression of myosin heavy chain (MyHC), myogenin, and myogenic determinant MyoD, thereby increasing myotube diameter. In vivo, it can improve doxorubicin-induced skeletal muscle atrophy in mice and repair muscle fiber damage caused by natural aging nematodes. The chemotherapeutic drug is doxorubicin, which can exert an anti-atrophy effect by maintaining the expression of myosin heavy chain (MyHC) and improving myotube morphology damage. This invention is the first to discover and experimentally demonstrate that *Hirudo medicinalis* freeze-dried powder has the activity of promoting myoblast differentiation and preventing muscle atrophy, opening up new applications for traditional Chinese medicine leeches in the prevention and treatment of muscle atrophy and expanding the medicinal value of *Hirudo medicinalis*.

This invention discloses a multifunctional injectable hyaluronic acid hydrogel, its preparation method, and its applications. The hydrogel comprises: a methacrylamide hyaluronic acid hydrogel matrix; cobalt-containing composite microspheres dispersed in the matrix, wherein the cobalt-containing composite microspheres are gelatin methacrylate / alginate methacrylate microspheres loaded with citric acid-coated cobalt nanoparticles; and miR-206-3p-enriched exosomes loaded in the hydrogel. The multifunctional injectable hyaluronic acid hydrogel provided by this invention organically integrates the hyaluronic acid matrix, cobalt-containing functional microspheres, and miR-206-3p-enriched exosomes, and can simultaneously provide multiple functions such as structural support, local gelation, angiogenesis promotion, and myogenic differentiation promotion. It overcomes the shortcomings of single materials in meeting the comprehensive repair needs of acute skeletal muscle injury, and provides a comprehensive repair strategy for acute skeletal muscle injury that combines structural support, angiogenesis induction, myogenic differentiation regulation, and anti-fibrosis functions.

The present invention relates to a method for producing a composition which comprises animal protein, comprising (a) isolating precursor cells from perinatal tissue of a mammal; (b) incubating the precursor cells under conditions which lead to a myogenic differentiation of the precursor cells; and (c) harvesting the cells. The present invention also relates to a method for producing precursor cells from perinatal tissue, to animal protein produced according to the invention, and precursor cells produced according to the invention. The present invention further relates to the use of a culture medium which has a reduced content of methionine in comparison to standard medium, to the differentiation of precursor cells, and to a method for the in vitro production of a meat-like composition.

This invention belongs to the field of cultured meat and relates to a method for constructing a cultured meat scaffold. The invention involves preheating a deionized aqueous solution of soybean protein and then cooling it to room temperature to obtain a soybean protein solution; dissolving fish gelatin in deionized water to obtain a fish gelatin solution; mixing the soybean protein solution and the fish gelatin solution, stirring until homogeneous to obtain a mixed solution; then adding glutaminase solution; heating and incubating to inactivate the enzyme; and after full hydration, obtaining a gel scaffold, which is then ready for consumption as a dual-protein cultured meat scaffold. Preheating and enzymatic cross-linking are used to regulate the dual-protein gel network, reducing its heterogeneity and improving its stability and strength. The dual-protein gel scaffold possesses excellent mechanical properties, a uniform network structure, and biocompatibility, which can improve the adhesion, proliferation, and spreading area of ​​porcine skeletal muscle satellite cells, while simultaneously meeting the requirements for myoblastic differentiation.

The invention discloses a serum-free culture medium and application thereof in culture of large yellow croaker muscle stem cells. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, wherein the exogenous additive components comprise HEPES, sodium chloride, hydroxypropyl-beta-cyclodextrin, sodium metavanadate, vitamin C magnesium phosphate, omega-3 polyunsaturated fatty acid, pyridoxine hydrochloride, nicotinamide, cholesterol, calcium chloride, potassium chloride, a soybean pancreatin inhibitor, taurine, trehalose, ethanolamine, a telomerase activator and recombinant human serum albumin. The animal-derived protein-free serum-free culture medium disclosed by the invention does not contain animal-derived components such as serum, does not need to add components such as growth factors or hormones, not only has definite chemical components, high safety, low cost and high cell proliferation speed, but also can effectively improve the myogenic differentiation potential of muscle stem cells and effectively activate telomerase activity to solve the problem of cell aging; and a feasible product and a feasible method are provided for culture of fish cells and large-scale production of cell culture meat.

The application discloses a kind of biological absorbable double-layer heterogeneous membranes for promoting tendon-bone interface healing, preparation method and application, belong to the field of biomedical materials.Using solution casting method, freeze-drying technology and photoetching technology, a kind of absorbable double-layer membrane with asymmetric structure is prepared by taking natural polymer material chitosan and gelatin as main raw materials.The groove orientation side of the membrane wraps tendon / ligament graft, which can effectively arrange and lengthen fibroblasts at the interface, promote cell myogenic differentiation and reduce scar hyperplasia tissue generation caused by disordered arrangement;The porous sponge side is attached to the bone tunnel, which can improve cell adhesion and proliferation capacity, and promote bone formation at the interface.The material has good flexibility, can provide certain mechanical properties for the affected area, can be degraded in vivo, and the product has no toxic and side effects on the body.The two structures jointly promote tendon-bone interface healing and reduce adverse reactions.The material has no external drug stimulation, good biological safety, low price, simple preparation process, strong operability, good adjustability for different affected areas, strong clinical execution ability, and has wide application prospect.

The invention belongs to the field of preparation of medical materials, and particularly relates to application of acellular matrix loaded Sr-Se coated SiO2 and adipose-derived mesenchymal stem cells in sequential treatment of volumetric muscle defects. On the basis of the Sr-Se-coated SiO2 nano composite material, a composite filling scaffold is developed in combination with a tissue engineering scaffold, and the effect of the composite filling scaffold in the VML treatment process is researched. The filler is composed of three parts, namely a nano material Sr-Se coated SiO2 with multiple functions of resisting inflammatory response, promoting vascularization and the like, ADSCs with myogenic differentiation potential and a dECM composite scaffold used for loading contents, the composite filling scaffold provided by the invention can improve the problem that traditional VML treatment is difficult, and sequential treatment of personalized guide VML is achieved.

This invention relates to the field of tissue engineering technology and discloses a multi-level oriented muscle repair scaffold made from chitin nanocrystals and its preparation method. The invention uses chitin derived from shrimp and crab shells as raw material to prepare chitin nanocrystals. Through steps such as preparing an aqueous suspension of chitin nanocrystals, ultrasonic dispersion, directional freezing to induce ice crystal orientation growth, freeze-drying to control ice crystal sublimation, and glutaraldehyde cross-linking fixation, a multi-level oriented scaffold with a macroscopic oriented pore structure and a microscopic oriented micro / nanofiber structure is prepared, with the chitin nanocrystals arranged along the orientation pore direction. This scaffold has a high specific surface area, can promote the orientation alignment and myogenic differentiation of C2C12 cells, and can be effectively used for the repair of volumetric muscle tissue loss. Furthermore, the preparation method is simple, requires low-end equipment, is inexpensive, and can be mass-produced.

This invention relates to the fields of improving the immune microenvironment, promoting angiogenesis and bone regeneration, and specifically to a multifunctional nanomaterial for rotator cuff injury complicated with osteoporosis and its preparation method. This invention develops a multifunctional nanomaterial, Nb2C@Mg-MOF (denoted as NM), for rotator cuff injury complicated with osteoporosis. This nanomaterial exhibits good biocompatibility, can scavenge reactive oxygen species, effectively inhibits inflammatory responses, adipogenic differentiation and osteoclastogenesis, promotes angiogenesis, osteogenic and myogenic differentiation, and can effectively inhibit the progression of osteoporosis and fatty infiltration, while promoting rotator cuff tendon-bone healing.