Oligonucleotide for promoting repair of skeletal muscle injury, medicine and application thereof

An oligonucleotide and skeletal muscle technology, applied in the field of promoting the repair of skeletal muscle damage, can solve the problems of poor curative effect and long treatment process, and achieve the effect of low cost, good recovery of skeletal muscle function, and promotion of regeneration and repair

Inactive Publication Date: 2018-11-02
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional treatment methods for skeletal muscle injury include rest, ice compress, pressurization, raising the affected limb, hyperthermia, water bath, etc. The treatment process is long and the curative effect is poor
At present, there is no particularly effective treatment method for skeletal muscle injury in clinical practice.

Method used

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  • Oligonucleotide for promoting repair of skeletal muscle injury, medicine and application thereof
  • Oligonucleotide for promoting repair of skeletal muscle injury, medicine and application thereof
  • Oligonucleotide for promoting repair of skeletal muscle injury, medicine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 object oligonucleotide promotes skeletal muscle myoblast myogenic differentiation

[0031] According to the high-throughput analysis of gene transcripts in various mouse tissues, it was found that the transcripts of the H19 gene were significantly highly expressed in adult skeletal muscle, and this result was verified by q-PCR. Based on this, the scientific hypothesis that the H19 imprinted gene, which functions as RNA, regulates the development and function of skeletal muscle is put forward. An oligonucleotide with potential function was screened from the H19 transcript by using the public gene function analysis software, and the mouse skeletal muscle myoblast cell line C2C12 was used as an in vitro model to investigate the effect of the target oligonucleotide on the proliferation of C2C12 cells differentiation.

[0032] C2C12 cell culture: the cell line was purchased from ATCC Company in the United States, and cultured according to the method published b...

Embodiment 2

[0052] Example 2 Objective oligonucleotide inhibits the expression of Smad4 in skeletal muscle myoblasts

[0053] The study found that the target oligonucleotide can effectively promote the myogenic differentiation of skeletal muscle myoblasts, suggesting that the target oligonucleotide may have the function of promoting the repair of skeletal muscle damage by regulating the cycle proteins of skeletal muscle cells. Studies have shown that TGF-β-Smad signaling pathway plays an important regulatory role in the process of skeletal muscle injury healing. Members of the Smad protein family, as receptor kinases downstream of TGF-β, play an important mediating function in the TGF-β signaling pathway. According to structure and function, Smads are divided into 3 subfamilies: receptor activating type (including Smad1 / 2 / 3 / 5 / 8), receptor inhibitory type (including Smad6 / 7) and general type (Smad4). The synergy between Smad4 and other Smad protein molecules is a key link in the TGF-β sup...

Embodiment 3

[0081] Example 3 Microinjection of the target oligonucleotide at the wound edge can significantly promote the regeneration of damaged skeletal muscle fibers

[0082] Based on the above examples, it is found that the target oligonucleotide has the function of promoting the myogenic differentiation of C2C12 cells, and it is speculated that the exogenous addition of the target oligonucleotide to the injured skeletal muscle may accelerate the myogenic differentiation process of the proliferated skeletal muscle myoblasts , thereby promoting the fusion and structural reconstruction of new muscle fibers. Therefore, it is planned to screen out the optimal plan of using the target oligonucleotide, observe the regeneration process of the damaged skeletal muscle by HE staining and immunohistochemistry, analyze the size of the new skeletal muscle fiber, and evaluate the therapeutic effect of the target oligonucleotide.

[0083] (1) Preparation of skeletal muscle injury regeneration model:...

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Abstract

The invention relates to the field of biomedicine and particularly relates to oligonucleotide for promoting repair of skeletal muscle injury and an application method thereof. The invention firstly discloses oligonucleotide which is screened from transcripts of human-derived growth imprinted gene H19 and has a function of obviously promoting myogenic differentiation of skeletal-muscle myoblast. The oligonucleotide is characterized by being shown in sequences of SEQ ID NO:1 and SEQ ID NO:2. The oligonucleotide and the application method have the advantages of simple operation, no risk, promotion for fast regeneration of new muscle fiber after skeletal muscle injury and good function restoration. Therefore, the oligonucleotide and the application method have good application prospect in genetherapy of skeletal muscle injury caused by acute contusion, crush injury, explosion injury or chemical substances.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an oligonucleotide with the function of regulating the myoblast differentiation of skeletal muscle, a medicine and an application method for promoting the repair of skeletal muscle damage. Background technique [0002] Skeletal muscle injury is a common disease caused by accident trauma, disaster injury, sports injury or injury caused by chemical poisons. The natural healing process after skeletal muscle injury is slow, the healing ability is limited, often accompanied by muscle fiber scar formation, functional recovery is often incomplete, and severe cases even lose exercise capacity. The traditional treatment methods for skeletal muscle injuries include rest, ice compress, pressurization, raising the affected limb, hyperthermia, water bath, etc. The treatment process is long and the curative effect is poor. At present, there is still no particularly effective treatment f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11A61K31/713A61P21/00
CPCA61K31/713A61P21/00C12N15/11C12N2310/10
Inventor 王艾平冉曦申明强龙爽王涛王军平粟永萍程天民
Owner ARMY MEDICAL UNIV
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