213results about How to "Increase biological function" patented technology

The invention relates to mRNA comprising lipid nanoparticles and their medical uses. The lipid nanoparticles of the present invention comprise a cationic lipid according to formula (I), (II) or (III) and/or a PEG lipid according to formula (IV), as well as an mRNA compound comprising an mRNA sequence encoding an antigenic peptide or protein. The invention further relates to the use of said lipid nanoparticles as vaccines or medicaments, in particular with respect to influenza or rabies vaccination.

The invention discloses an acellular matrix with natural level of glycosaminoglycan and preparation and application thereof. The preparation method comprises the following steps: carrying out acellular disposal on natural biological tissue to obtain an acellular matrix; detecting missing amount of glycosaminoglycan in the acellular matrix; and supplementing glycosaminoglycan by means of natural ingredients in the acellular matrix to obtain the acellular matrix with natural level of glycosaminoglycan. Through the method, a lot of the glycosaminoglycan component in the acellular matrix is recovered. The acellular matrix has good biocompatibility. The natural biological structure is recovered, and the acellular matrix is beneficial to cell growth. glycosaminoglycan is supplemented for the acellular matrix, and a tissue engineering carrier is constructed. According to the invention, a biological carrier which meets cell growth requirement and mechanical strength requirement and has similar structural components as a natural extracellular matrix can be prepared rapidly. The invention is a great breakthrough of the tissue engineering construction technology. Meanwhile, principle of the invention is reliable, the technology is simple and flexible, product reproducibility is good, and industrialization is easy to realize.

The invention discloses pure-titanium metal surface micro-arc oxidation treatment electrolyte and an antimicrobial bioactive coating preparation method thereof, belongs to a medical titanium metal surface modification technology, and aims to provide a micro-arc oxidation treatment electrolyte which can remarkably improve pure-titanium metal surface bioactivity and coating bonding strength and a coating preparation method thereof. The electrolyte comprises a compound system including the components of calcium acetate, sodium phosphate and a zinc oxide precursor, wherein the phosphorous ion concentration is 0.5 to 1.0 M, the molar ratio of Ca to P is 0.2 to 1.0, and the designed Zn<2+> concentration accounts for 10 to 20 percent of the total concentration. A biological composite coating is directly generated on the surface of a titanium metal surface in-situ by adopting the electrolyte and a micro-arc oxidation technology. The coating has high bonding strength with a substrate, dense inner layer, coarse and porous outer layer, high bioactivity and high antibacterial property; moreover, the used raw materials are easy to obtain; the process is simple and easy to operate; and the electrolyte has stability, reusability, and low production cost and is suitable for industrial production.

Disclosed is a 3-D bioreactor to culture hepatic cells, comprising a culture cavity provided with a cell feeding port and a vent, an inflow cavity with a culture fluid inlet and an outflow cavity with a culture fluid outlet which are arranged next to the culture cavity. A plurality of hollow fibers are arranged inside the culture cavity, each with one end communicated with the inflow cavity and the other end communicated with the outflow cavity, forming a culture fluid flow passage which is communicated with the culture cavity through the tiny holes on the hollow fibers. The invention is characterized in that a 3-d bracket with a plurality of holes is arranged inside the culture cavity. The invention can provide a growth and culture condition that accords better with the physiological environment for the hepatic cells, which is good for culturing dense hepatic cells with high activity so as to ensure the therapeutic effect of a bio-artificial liver with the hepatic cells as the core.

As described below, the present invention features methods for reprogramming somatic cells and related therapeutic compositions and methods.

The invention discloses a low-expression CYP7A1 hepatic cell and a constructing method thereof. A method for inhibiting the CYP7A1 expression level of the hepatic cell comprises the step of introducing an encoding gene of small-interference RNA which is used for inhibiting the CYP7A1 gene expression into the hepatic cell so as to inhibit the CYP7A1 expression level of the hepatic cell. A slow virus vector system can be used for introducing the encoding gene of small-interference RNA which is used for inhibiting the CYP7A1 gene expression into the hepatic cell. An experiment proves that the method of the invention can obviously regulate the expression level of the CYP7A1 gene of the hepatic cell in an mRNA level and a protein level downwards and can further effectively reduce the secretoryvolume of total bile acid. The Low-expression CYP7A1 hepatic cell and the constructing method thereof can be used for the fundamental research and the clinical application of biologic artificial liver supporting treatment and have a wide application prospect.

In general, this invention provides methods and compositions for stabilizing, repairing, or replacing damaged or diseased tissues or organs by engineering blood vessels in such tissues or organs. In addition, the invention further provides methods and compositions for producing functional microvascular networks useful in tissue engineering.

The invention relates to a polysaccharide artificial blood vessel and a preparation method and application thereof. The polysaccharide artificial blood vessel is characterized in that the compact artificial blood vessel is prepared from water-insoluble polysaccharide fibers and colloidal solution which are prepared from polysaccharide; the polysaccharide is one or two of carboxymethylcellulose, hydroxy propyl cellulose, hydroxyethylcellulose, hyaluronic acid, alginate, heparin, chondroitin sulfuric acid, chitin, carboxymethyl chitin, hydroxypropyl chitin, hydroxyethyl chitin, carboxymethyl hydroxypropyl chitin, carboxymethyl hydroxyethyl chitin, hydroxypropyl hydroxyethyl chitin, sulfonated chitin, chitosan, carboxymethyl chitosan, hydroxypropyl chitosan, hydroxyethyl chitosan, carboxymethyl hydroxyethyl chitosan, carboxymethyl hydroxypropyl chitosan, hydroxypropyl hydroxyethyl chitosan, sulfonated chitosan or succinyl chitosan. The polysaccharide artificial blood vessel has the high compactness, stretchability, bendability, mechanical strength and flexibility, can be implanted into bodies during the operation of trauma repair, and is used as artificial blood vessels to replace hurt vessels and improve blood-supply condition.

The invention provides a zinc-containing artificial bone for bone tissue repair and a preparation method thereof. The zinc-containing artificial bone is prepared from the following raw materials in percentage by mass: 0.1-15% of zinc-containing powder, 80-98% of bioceramic powder and 0.5-10% of high-molecular polymer adhesive. The preparation method of the zinc-containing artificial bone comprisesa 3D printing technique. The method solves the problems of poor bone inductivity and poor bone formation promoting property in the traditional bioceramic artificial bone, and solves the problems of poor mechanical strength and difficulty in satisfying the bone repair demands of the bearing part in the traditional bioceramic artificial bone.

The invention discloses a preparation method of a cordyceps sobolifera and burdock oral liquid, and belongs to the technical field of extraction of nutritional components of plants. The preparation method of the cordyceps sobolifera and burdock oral liquid comprises the following steps: (1) preparing cordyceps sobolifera cordyceps liquid seeds; (2) cultivatiing cordyceps sobolifera cordyceps; (3) extracting after the materials are smashed with hot water and carrying out homogenate; (4) carrying out high shear and superfine grinding with wet method; (5) hydrolyzing cellulose, amylase, and pectinase; (6) filtering, concentrating and filling to obtain the cordyceps sobolifera and burdock oral liquid. The preparation method is short in product production cycle, avoids raw material loss in product production process and waste discharge in production process, and is an environment-friendly production method with a high product output rate and simple production technology; besides, as health care type functional food, the cordyceps sobolifera and burdock oral liquid can be drunk for a long time as daily health care, can be used as a product for the adjuvant therapies of related diseases, and broadens the application fields of cordyceps sobolifera cordyceps and burdock root.

Compositions and methods for identifying new treatments for Facioscapulohumeral muscular dystrophy (FSHD), and uses thereof.

The invention provides a fruit and vegetable type whole grain beverage and a preparation method thereof. The fruit and vegetable type whole grain beverage is prepared from the following raw materials in percentage by weight: 30%-40% of fresh fruit and vegetable juice, 30%-40% of pearl barley, 10%-20% of soybeans, 4%-16% of walnut meat, 1%-2% of a complex enzyme preparation, 1%-2% of yeast group series, 1%-2% of lactobacillus series and purified water in a weight ratio of 100 to 400. The fruit and vegetable type whole grain beverage integrates the advantages of fruit, vegetable and lactic acid fermentation, is increased in content of nutrients such as vitamin C, is rich in a large quantity of active peptides with a unique physiological activity function, adjusts structures of micro-ecological bacterial groups in a human body, and improves the digestibility of the human body, the biological function of lactic acid bacteria and the immunity of the human body.

A method of processing coffee beans in which green coffee beans are roasted and roasted coffee beans are obtained, wherein the green coffee beans are brought into contact with a high-temperature, high-pressure fluid and the roasting process is performed.

The invention discloses new application of sodium houttuyfonate in pharmacy, in particular to the application of the sodium houttuyfonate in preparing medicaments for preventing and treating myocardial hypertrophy and/or ventricular hypertrophy. The application is to prepare an in-vitro myocardial cell hypertrophyby adopting three methods of preparing an in-vivo animal model of myocardial hypertrophy and ventricular hypertrophy, culturing a myocardial cell in vitro and inducing epinephrine by isoproterenol, L-thyroxine and abdominal aorta banding. Experiment results prove that the sodium houttuyfonate can reduce the increase of left cardiac index and global cardiac index of a model animal, inhibit hypertrophy of an in-vitro myocardial cell, reduce the cAMP concentration of plasma, the content of Ang II and ET-1 of a myocardial tissue and the content of TNF-alpha and ALD of serum, and can reduce blood pressure and decrease heart rate. Experiment results suggest that the sodium houttuyfonate has the functions of resisting myocardial hypertrophy and ventricular hypertrophy, and the mechanism of the sodium houttuyfonate can be related to the inhibition of sympathetic activity, blockage of system activity of feritin-hypertensin-aldosterone, and effect of endocrine factors.

The invention relates to a preparation method of a graphene-oxide-based hybrid biological functional coating. The preparation method comprises the following steps: 1, preparing a graphene oxide/nanosilver composite material; 2, treating a titanium sheet; 3, grafting the titanium sheet with the graphene oxide/nanosilver composite material; 4, preparing a graphene oxide/nanosilver/collagen hybrid coating. The preparation method has the advantages as follows: (1) by a method for reducing nanosilver by using ultraviolet, the uniformly-dispersed nanosilver with a small size can be obtained, and adverse effects on the environment are avoided; (2) on the basis of graphene oxide, graphene oxide, collagen and nanosilver are organically combined, and through coordinated action of the three components, respective shortcomings can be improved, so that the graphene-oxide-based hybrid biological functional coating has a more excellent biological function; (3) when the preparation method provided by the invention is used for preparing the graphene oxide-based hybrid biological functional coating, the equipment investment and the implementing difficulty are low; the preparation method is simple and feasible; by the preparation method, too much influence on the environment is avoided.

The invention discloses expression and preparation methods for a polyvalent multi-specific antibody and an immune hybrid protein and belongs to the technical field of biology. According to the invention, by using characteristics of protein Intein, a novel method for preparing polyvalent specific antibodies, or antibody and cell factor merged hybrid immunoproteins, or antibody and toxin protein merged immunotoxins or antibody and other active protein merged immune hybrid proteins is designed and developed. According to the methods, products, i.e., the polyvalent specific antibody and the immune hybrid protein are prepared through expressing all parts of the hybrid protein separately in appropriate procaryotic or eucaryotic cell systems, carrying out high-performance affinity-chromatography purification and separation, and then, carrying out splicing in vitro under the condition of intein splicing. The methods have high production efficiency, are wide in applicable application range and facilitate the separation and purification of the products. The invention provides a novel method for preparing biological drugs for directional attack on cancers or other diseases.

The invention relates to the technical field of biomaterials, and particularly relates to a bone repair regeneration material and a preparation method thereof. The bone repair regeneration material comprises poly-DL-lactic acid, a calcium phosphate composition and collagen, wherein the calcium phosphate composition comprises hydroxyapatite and beta-tricalcium phosphate. According to the bone repair regeneration material and the preparation method thereof, the poly-DL-lactic acid, the hydroxyapatite-beta-tricalcium phosphate and the collagen are applied to construct the poly-DL-lactic acid/hydroxyapatite-beta-tricalcium phosphate/collagen bone repair regeneration material. The phase size is small and the uniformity is high in a preparation process, so that the prepared material has uniform distribution of material composition, has good biological function and mechanical properties, and provides a three-dimensional adhesion and growth space. The method is simple and safe, low in economic cost and adjustable in pore size and porosity, and the degradation rate of a scaffold can be controlled in a wide range. The bone repair regeneration material is used as a bone tissue engineering scaffold material, and is applied in repair of different kinds of bone tissue defects.

Bio-medical implanting material with biomimetic nano film and its use are both disclosed herewith. The implanting material uses NiTi shape-memory alloy as base with biomimetic nano TiO2 bio-ceramic film of 100-200nm to stop Ni ions liberated in human body liquid. It can be used as implanting device, e.g. tubular support, bone fixer, bone orthotic device, and oral orthodontic material, etc.

The invention provides a superhydrophilic coating with the function of catalyzing NO long-period stable release and a preparation method thereof. The preparation method comprises the following steps of performing pretreatment on the substrate material surface; putting the treated substrate material in partial acid buffer liquid; then, adding polyphenol compounds, multi-amine compounds, NO catalysts and oxidants for reaction; soaking a product obtained through reaction in deionized water; performing ultrasonic cleaning; then, performing drying under the nitrogen gas condition; preparing the superhydrophilic coating. The method has the advantages that the operation is simple; the reaction conditions are mild; the prepared coating material contains polyphenol compounds, a large number of functional groups such as carboxyl, phenolic hydroxyl groups, benzoquinonyls and amido groups and metal ions or chemicals capable of catalyzing an NO donor to release NO; the metal ions and the chemicalsmutually act with phenolic hydroxy functional groups or act with a system Pi-Pi accumulation for realizing the long-period existence of NO catalysts; further, the long-period sustained release function of NO is realized; the superhydrophilic coating can be used for preparation of materials for resisting blood coagulation, resisting hyperplasia, promoting endothelium growth, resisting tumor and thelike.

The invention belongs to the traditional Chinese pharmaceutics field, and relates to an application of traditional Chinese medicine radix scrophulariae to the preparation of a drug for preventing and curing cardiac hypertrophy, hypertrophic cardiomyopathy and chronic heart failure. The invention researches the effect of the cardiac hypertrophy and ventricle reconstruction model of a plurality of mice and rats on the traditional Chinese medicine radix scrophulariae. The experimental results show that the radix scrophulariae water or the alcohol abstracts has evident function for preventing the cardiac hypertrophy, the ventricular remodeling and the chronic heart failure caused by various reasons, the mechanism is obviously related with the excessive activation of the neuroendocrine system such as a suppressing renin-angiotensin-aldosterone system and is related with the functions of blood pressure reduction, heart beat slowing and hemodynamics improvement. The figwort can be used for preparing the drug for preventing the diseases of cardiac hypertrophy, hypertrophic cardiomyopathy and chronic heart failure and for reducing the possibility of the incidence rate of arrhythmia and the sudden death of patients with cardiac hypertrophy, hypertrophic cardiomyopathy and chronic heart failure.

The invention relates to novel antibacterial restorative functional dressing and a preparation method thereof. The novel antibacterial restorative functional dressing is formed by crosslinking and compounding chitosan and collagen on a bacterial cellulose membrane through a cross-linking agent, wherein a mass ratio of the chitosan to the bacterial cellulose membrane is (0.001-5) to 100; a mass ratio of collagen to the bacterial cellulose membrane is (0.5-20) to 100; a mass ratio of the chitosan to the collagen is (0.001-5) to (20-0.5); and the collagen is a mixture of collagen with small molecular weight and collagen with large molecular weight according to a ratio of 1 to 1. The antibacterial restorative functional dressing has very good biocompatibility, cellular affinity and mechanical property, has excellent water-permeable, gas-permeable or drainage and moisture absorption effect, has the effects of realizing antisepsis and anti-inflammation, reducing wound infection and quickly relieving pain of the wound, and has the effects of relieving chromatosis and promoting the wound to heal during a wound healing period. Meanwhile, the dressing can naturally drop along with epithelial regeneration, so that the treatment time and cost is reduced, and the wound infection probability is reduced.