92 results about "Spermine" patented technology

The invention relates to the technical field of biological detection, and discloses a mycoplasma pneumoniae nucleic acid rapid detection method, which comprises the following steps: mixing a sample to be detected with a multifunctional pretreatment buffer solution containing tris (hydroxymethyl) aminomethane, potassium acetate, polyethylene glycol, saponin, spermine tetrahydrochloride and tris (2-carboxyethyl) phosphine hydrochloride, and carrying out cracking treatment; a cracking mixed solution is obtained; redissolving the enzymatic isothermal amplification basic reagent, the specific primer and the probe to obtain a redissolving amplification system; sucking the supernatant of the cracking mixed solution, adding the supernatant into a redissolving amplification system, and adding a magnesium acetate solution to obtain a reaction mixed solution to be detected; and carrying out isothermal amplification and signal acquisition to obtain a detection conclusion. The multifunctional pretreatment buffer solution is used for cracking the to-be-detected sample, sample liquefaction, cracking and nucleic acid release are completed in a single system, the nucleic acid extraction and purification step of centrifugal column adsorption or magnetic bead elution is avoided, and the operation process is simplified.

The invention discloses a fluorescent probe for detecting spermine and spermidine in a water sample and urine as well as a preparation method and application of the fluorescent probe, and relates to the technical field of supramolecular fluorescent probes, and the fluorescent probe is formed by reacting 14-membered cucurbit urils (tQ [14]) and a compound (E)-1-(4-boron benzyl)-4-(4-hydroxyl styryl) quinoline-1-ammonium bromide according to a molar ratio of 1: 1. The novel fluorescent probe provided by the invention can specifically detect spermine and spermidine in a water sample and urine, and the preparation method of the fluorescent probe is simple and easy to implement; the method for detecting spermine and spermidine in a water sample and urine by using the fluorescent probe has the advantages of high sensitivity, high selectivity, low cost, simple sample treatment, convenient operation, rapid determination and the like.

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

The invention relates to the technical field of forest cultivation, and discloses a carya illinoensis fruit forest variety configuration method which comprises the following steps: configuring a main cultivation variety and a pollination variety in a fruit forest; preparing a pollination synergistic composition; the composition is sprayed at the initial stage of stigma exposure of female flowers of a main cultivated variety. The composition comprises a phenolic acid grafted carboxymethyl chitosan derivative, low methoxyl pectin, a calcium ion cross-linking agent, sugar alcohol chelated boron and spermidine. According to the method, an interfacial film with antioxidant activity is formed on the surface of the stigma by using the grafting modified matrix, so that the stigma browning is effectively inhibited, and the pollination period is prolonged; by constructing a calcium cross-linked low-methoxyl pectin interpenetrating network, an interfacial film is endowed with an intelligent degradation characteristic of enzyme-sensitive response to pollen, and protection at ordinary times and immediate dissolution after touching flowers are realized. According to the method, pollen is captured through static electricity, nutritional factors are released in a targeted mode, the pollination and fertilization efficiency is remarkably improved, the sterility risk in the flowering phase is relieved, and the yield and quality of carya illinoensis are improved.

The invention discloses application of spermidine in in-vitro culture of human unfrozen oocytes and embryos. By adding the combination of spermidine, melatonin and astaxanthin into the thawed oocyte resuscitation culture solution and the embryo in-vitro culture solution, the mitochondrial autophagy and mitochondrial functions of the thawed oocytes are improved, so that the in-vitro culture effect of the thawed oocytes and embryos is improved, and the success rate of an assisted reproduction technology is increased.

The invention discloses an SERS (Surface Enhanced Raman Scattering) flexible sensor based on dual-ion modification as well as a preparation method and application of the SERS flexible sensor, and belongs to the technical field of biomedical detection. A bacterial cellulose membrane (BCM) is used as a base material, cation modification (QBCM) is carried out through glycidyl trimethyl ammonium chloride (EPTMAC), anion modification (TABCM) is carried out through tannic acid (TA), a functional substrate with asymmetric charge distribution and a porous structure is constructed, then Ag NPs are loaded on the modified base material through an in-situ reduction method, the QBCM-coated AgNPs and TABCM-coated AgNPs dual-ion active substrate is formed, and the AgNPs / TABCM-coated AgNPs dual-ion active substrate is used for preparing the composite material. The SERS flexible sensor is integrated through a layer-by-layer self-assembly (LbL) technology; the SERS flexible sensor can realize specific enrichment and synchronous detection of anionic kynurenic acid (KYNA) and cationic spermine (Spm) in urine, and is low in detection limit and good in reproducibility.

The invention discloses a composition for promoting bulb expansion of hippeastrum hippeastrum, the composition is an aqueous solution, the solute in the composition comprises putrescine, spermidine, spermine, triacontanol, ethanol, Tween 80, sodium lignin sulfonate and ethylene glycol, and the dosage ratio of the materials is 10: 1: 2: 0.05: 10: 5: 3: 2 according to the dosage weight. And diluting the composition with water to obtain a diluent with the mass percent concentration of 0.04-0.06%. The diluent is used for foliage spraying and seedball base irrigation in the germination stage, the growth stage, the flowering stage and the post-flowering rejuvenation stage of hippeastrum hippeastrum, the seedball diameter of hippeastrum hippeastrum can be remarkably increased, and economic benefits are increased.

The invention relates to the technical field of rice stress-resistant cultivation, and discloses a rice root system salt tolerance adjusting method based on exogenous spermidine, which comprises the following core steps: in the critical period of rice root system development, directionally applying a spermidine solution subjected to purity verification to a rice root system area, by accurately regulating and controlling the application concentration, frequency, duration and application environment conditions of spermidine, the osmotic regulation capability and the anti-oxidation system function of a rice root system in a high-salt environment with the soil conductivity larger than or equal to 2 dS / m are improved, the salt tolerance of the root system is remarkably improved, and then growth and development of rice are guaranteed. The spermidine is a straight-chain polyamine compound with good biocompatibility, the chemical purity is not lower than 98%, no heavy metal is left, and the contents of lead, cadmium and mercury are all smaller than or equal to 0.1 mg / kg. According to the invention, precise targeted regulation and control of the salt tolerance of the rice root system are realized, and the regulation effect is stable and remarkable.

This invention discloses the application of engineered *Citrobacter freundii* in the fermentation synthesis of spermine. The engineered *Citrobacter freundii* is a strain overexpressing the polyphosphate kinase gene Ppk1. The engineered *Citrobacter freundii* is inoculated into a fermentation medium for fermentation culture to synthesize spermine. The synthesis and extraction method of this invention for producing spermine is simple to operate, highly reproducible, low in cost, and yields a high spermine content in the fermented bacterial cells, accounting for 12.42% of the bacterial dry weight. The extracted spermine has a purity as high as 90.26%.

The present application relates to the technical field of cosmetic biotechnology and nano-carrier drug delivery, and provides PDRN-putrescine co-delivery nano-liposome as well as a preparation method and application thereof.The PDRN-putrescine co-delivery nano-liposome provided by the present application comprises the following components in mass fraction: 0.1-3% of polydeoxyribonucleotide; 0.1-3% of putrescine; 2-8% of phospholipid emulsifier; 0-15% of polyhydric alcohol cosolvent; and the balance is a buffer solution; and the buffer solution has a pH value of 6-7.5.The present application realizes stable co-encapsulation of PDRN and putrescine by constructing a specific buffer system to regulate the pH value, using the amphiphilic property of the phospholipid bilayer and the in-situ ion pair technology, solves the compatibility problem of PDRN and putrescine, significantly improves the transdermal absorption rate of PDRN through transmembrane delivery of the nano-carrier, and realizes the synergistic repair and anti-aging effect of PDRN and putrescine at the cellular level.

Disclosed in the present invention are a cross-linked polyamide hydrogel material and a preparation method therefor. The hydrogel is obtained by means of a cross-linking reaction between a water-soluble polyamide and a cross-linking agent. The water-soluble polyamide comprises poly(malic acid-spermidine), poly(malic acid-spermine), poly(succinic acid-spermidine), or poly(succinic acid-spermine). The cross-linking agent is an endogenous dibasic acid. The cross-linking reaction means that after being activated by a catalyst, a carboxyl group on the endogenous dibasic acid undergoes a two-step activation cross-linking reaction with an imine group on the backbone of the polyamide to obtain the hydrogel. The present invention prepares the hydrogel by means of a two-step activation cross-linking technique. By means of the cross-linking technique, the amounts of the catalyst and the cross-linking agent can be reduced, and hydrogels having different properties can also be prepared by controlling the type of the cross-linking agent and cross-linking conditions, thereby improving the cross-linking reaction efficiency and achieving the control of the degradation rate of the hydrogel and the release rate of an active monomer, exhibiting broad application prospects.

The invention relates to an anti-aging composition, and belongs to the field of medicines and health-care product preparations. The composition is a microcapsule assembled by a water-soluble wall material, a first core material, a fat-soluble wall material and a second core material which are sequentially coated from outside to inside, and the first core material comprises beta-nicotinamide mononucleotide (NMN), nicotinamide ribose (NR), ergothioneine and trimethylglycine (TMG); the second core material comprises resveratrol, curcumin, pyrroloquinoline quinone (PQQ) and spermidine, and is dissolved in medium chain triglyceride (MCT oil) containing an antioxidant tocopherol. According to the dosage form, physical isolation and differentiated release of water-soluble and fat-soluble components are realized, and the problems of incompatibility among the components, poor stability, low bioavailability and the like are solved. Through the multi-target synergistic effect, the composition can effectively improve the NAD + level and enhance the mitochondrial function so as to improve the skin state and delay senescence.

The invention relates to the technical field of molecular biology, in particular to an anti-inhibition enhanced PCR (Polymerase Chain Reaction) buffer solution which comprises a basic solution and an enhancer, the basic solution is prepared from the following components with final concentrations: MgCl2 with the concentration of 1 to 8 mM, KCl with the concentration of 80 to 200 mM, (NH4) 2SO4 with the concentration of 10 to 50 mM, Tris-HCl with the concentration of 40 to 80 mM and glycerol with the volume ratio of 5 percent; the reinforcing agent is prepared from the following components in final concentration: 8 to 20mM of spermidine and 60 to 140nM of betaine. According to the anti-inhibition enhanced PCR buffer solution, the interference and inhibition of various substances in an excrement sample on a PCR result can be resisted through the synergistic effect of the spermidine reagent and the P betaine reagent in a certain concentration range, and the detection sensitivity is remarkably improved.

The invention belongs to the technical field of pharmacy, and particularly relates to application of spermidine in sepsis treatment. Experiments prove that spermidine can regulate mitochondrial biogenesis and inhibit mitochondrial excessive autophagy to remarkably improve mitochondrial quality, maintain mitochondrial homeostasis and relieve myocardial dysfunction and heart structure damage. In addition, spermidine can inhibit oxidative stress caused by excessive inflammatory response so as to recover normal functions of mitochondria. The spermidine has a potential treatment effect on sepsis and has a good clinical application prospect in treating sepsis and related diseases caused by excessive autophagy.

The application provides a paclitaxel molecular umbrella delivery system prodrug, a preparation method and application thereof. The paclitaxel molecular umbrella delivery system prodrug comprises a molecular umbrella target head part composed of two surface amphiphilic molecules connected by spermidine, a paclitaxel drug part, and a connecting group connecting the molecular umbrella target head part and the paclitaxel drug part through a disulfide bond; wherein the surface amphiphilic molecule is selected from cholic acid, deoxycholic acid or a sodium sulfonate thereof. Compared with paclitaxel, the paclitaxel molecular umbrella delivery system prodrug has significantly improved water solubility, lower toxicity to normal cells than free paclitaxel, and significantly improved safety; and the paclitaxel molecular umbrella delivery system prodrug has the following characteristics in a physiological environment: releasing active drugs in the high GSH / ROS microenvironment of tumor cells; and maintaining a prodrug state in normal cells.

The compositions and methods reduce the biological age of an adult animal and / or reduce the rate of increase in the biological age of an adult animal. More specifically, the present disclosure relates to administering formulations comprising an effective amount of spermidine, alone or in combination with a dietary regimen such as a standard diet, caloric restriction, elderly diet, or high protein diet. The preparation can simultaneously reduce the biological age of tissues, organs or physiological systems in adult animals in need; or treating, preventing or delaying the onset of organ disease, or the decline of organ function, or a condition associated therewith, in an adult animal in need thereof.

The application discloses a kind of bionic immune regulation double-network hydrogel and its preparation method and application, its preparation method is: GelMA-SPD and HAMA-NB are dissolved, join photo-crosslinking agent, crosslinking is carried out under light, obtain double-network hydrogel;GelMA-SPD is methacrylated gelatin-spermidine;HAMA-NB is methacrylated hyaluronic acid N-(2-aminoethyl)-4-[4-(hydroxymethyl)-2-methoxy-5-nitrophenoxy]-butyramide.The hydrogel prepared in the application has good biocompatibility, and the preparation process is simple, and can be degraded.The hydrogel of the application can be applied to tissue repair material or tissue engineering scaffold, and is expected to solve the immune rejection problem in the process of tissue organ transplantation, and is conducive to reducing inflammatory reaction and promoting the rapid healing of tissue trauma.

A linearized mRNA preparation system, use thereof, and a preparation method for preparing mRNA by using same. A linearization buffer system comprises: 0-150 mM Tris-Ac or phosphate, 0.5-150 mM divalent metal ions or trivalent metal ions, 0.1-0.75 U / μL restriction endonuclease, and 0-7.5 mM spermidine. The present invention provides a transcription system, which has higher translation efficiency and lower dsRNA generation compared with a conventional HEPES / Tris system, and a phosphate system can be used simultaneously with the novel All In One transcription system.

The invention belongs to the field of nano biological materials and tumor radioimmunotherapy, and particularly relates to a metal-polyamine nano compound as well as a preparation method and application thereof. The nano-composite is a core-shell structure nano-particle which is constructed by forming a hydrophobic core through coordination of divalent manganese ions and spermidine under the mediation of oleic acid and performing surface coating by adopting DSPE-PEG (Distearoyl Pyrrolidone-Polyethylene Glycol). Through coordination assembly of divalent manganese ions and spermidine and DSPE-PEG surface modification, a cGAS-STING pathway is synchronously activated to promote DCs maturation, the mitochondrial fatty acid oxidation function of CD8 + T cells is enhanced, and the radiotherapy-induced antitumor effect is enhanced.

This invention discloses an oral liquid containing astaxanthin and its preparation method, which is prepared from raw materials comprising the following weight percentages: 0.5%~2% astaxanthin burst beads, 8%~12% collagen peptides, 0.1%~0.3% sodium hyaluronate, 0.01%~2% elastin peptides, 0.01%~0.05% spermidine, 0.005%~0.02% ergothioneine, and 0.001%~0.01% pyrroloquinoline quinone. The astaxanthin burst beads are prepared using astaxanthin oil as the core material and sodium alginate and chitosan as the wall material. This invention, by using astaxanthin burst beads with higher stability and bioavailability in the oral liquid, combined with other ingredients, can improve the user's skin moisture content and elasticity index, reduce wrinkle depth, reduce skin yellowing, and better exert the effects of the oral liquid.

The present invention concerns the field of diagnostics. In particular, it relates to a method for analyzing a protein analyte in a heparin-treated sample comprising or suspected to comprise said protein analyte, said method comprising the step of contacting said heparin-treated sample with spermidine prior to analyzing the said protein analyte in the said sample. Moreover, contemplated are a method for assessing a cardiovascular disease or disorder, a neurological disease or disorder, a metabolic disease or disorder or cancer as well as kits and reagent compositions comprising spermidine to be used in the said methods.

The invention discloses a muscle anti-aging composition with spermidine combined with a wolfberry aqueous extract and application of the muscle anti-aging composition, and belongs to the technical field of application of bioactive substances. The composition is prepared by compounding spermidine and a Chinese wolfberry aqueous extract according to a specific ratio, and the Chinese wolfberry aqueous extract is prepared by an optimized aqueous extraction process, so that core active ingredients are reserved to a greater extent. The optimal mass ratio of spermidine to the wolfberry aqueous extract is 1: 2. Through a synergistic effect mechanism, the antioxidant capacity of senescent muscle cells is remarkably improved, and the muscle cell oxidative stress and senescence process induced by D-galactose is effectively inhibited. The composition is simple in preparation process and safe in components, can be widely applied to the fields of food, health care products and medicines, and provides a new solution for developing efficient and natural muscle anti-aging products.

This invention provides a composition comprising (i) chondroitin and / or collagen peptide and (ii) at least one agent selected from the group consisting of butyrate, a butyrate equivalent, trehalose, a trehalose equivalent, spermidine, and a spermidine equivalent. This invention also provides related pharmaceutical compositions, methods for ameliorating aging hallmarks in a subject, and articles of manufacture.

The invention belongs to the technical field of tobacco chemistry, and particularly relates to a method for detecting the content of spermidine compounds in tobacco leaves. The invention provides a method for determining the content of spermidine compounds in tobacco leaves, which adopts ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry to quantitatively detect the content of spermidine compounds in a sample solution to be detected by an external standard one-point method, the flowing phase B is selected from one or more of formic acid, acetic acid and a mixture of formic acid and water, the sample size is 0.5-3 [mu] L, the flow velocity is 0.2-1.0 mL / min, the elution mode is gradient elution, the ion source is an electrospray ion source, the scanning mode is positive ion scanning, and the detection mode is multi-reaction monitoring. According to the method, extraction conditions are optimized, the detection method has high sensitivity and accuracy, the content of multiple spermidine compounds in the tobacco leaf sample can be quantitatively detected at the same time, and the method is suitable for detection of large-batch tobacco leaf samples.

The invention belongs to the technical field of organic synthesis, and particularly relates to a preparation method of spermine. The method comprises the following steps: carrying out condensation reaction on Boc-beta-alanine and butanediamine which serve as raw materials under the action of a condensing agent to obtain an intermediate 1, carrying out reduction reaction on the intermediate 1 to generate an intermediate 2, namely N-[2-(ethylamino) ethyl] tert-butyl carbamate, and finally, carrying out BOC removal reaction and post-treatment on the intermediate 2 to obtain the spermine. The method is environment-friendly, pollution-free, low in cost, safe to operate, easy for industrial large-scale production, high in conversion rate and good in product quality.

The application provides application and methods of spermine in promoting germination and seedling growth of plants under drought conditions, and relates to the technical field of plant cultivation. The application of spermine and the method thereof provided by the application are simple in operation, can quickly and effectively promote the germination of alfalfa seeds and the growth of seedlings under drought stress, and are beneficial to popularization in the planting and production of alfalfa in arid areas.

The invention belongs to the field of chemical detection, and discloses a fluorescent probe P1, a preparation method thereof and application of the fluorescent probe P1 in spermidine and viscosity dual-function detection. The structural formula of the fluorescent probe P1 is shown in the specification. The recognition performance of the probe P1 in various common analytes in a DMF-HEPES buffer system is researched through a fluorescence spectrophotometer. Results show that the probe P1 has specific fluorescence recognition performance on spermidine, can overcome interference of other common analytes, and has a wide pH value application range. The probe has relatively good sensitivity on the recognition of spermidine, and the lowest detection limit is 0.213 mu M. In addition, the probe P1 has a good response effect on the viscosity of the solution, is not interfered by the polarity of the solution, and can realize quantitative detection of the viscosity. The results show that the probe P1 has wide application prospects in the fields of food safety, environmental detection and biology.

The invention discloses a method for synthesizing spermidine through a double-bacterium co-culture system, and relates to the technical field of bioengineering. The method comprises the following steps: constructing a first strain for synthesizing and secreting ornithine; a second strain is constructed and used for synthesizing aspartic semialdehyde and butanediamine, the second strain comprises carboxyl spermidine dehydrogenase and carboxyl spermidine decarboxylase, the carboxyl spermidine dehydrogenase is a mutant enzyme obtained by performing G13A and / or K159R mutation on an amino acid sequence as shown in SEQ ID NO: 1, and the carboxyl spermidine decarboxylase is a mutant enzyme obtained by performing G13A and / or K159R mutation on an amino acid sequence as shown in SEQ ID NO: 2. The carboxyl spermidine decarboxylase is a mutant enzyme obtained by carrying out D82E single-point mutation on an amino acid sequence as shown in SEQ ID NO: 2; and co-culturing and fermenting the first strain and the second strain to synthesize spermidine. According to the present invention, the metabolism division and the efficient synergy can be achieved, the metabolism load and the butanediamine toxicity accumulation can be reduced, and the catalytic efficiency of the key reaction and the cofactor utilization efficiency can be improved so as to significantly improve the spermidine yield and the conversion efficiency.

Disclosed is the use of spermidine as a biomarker for monitoring the treatment response in major depressive disorder (MDD). Also disclosed are methods for assessing a response to an MDD treatment in a patient having MDD, antidepressants for use in treatment of MDD and methods of treating MDD.