253 results about "Spermine" patented technology

The invention discloses an immunoregulation hydrogel as well as a preparation method and application thereof, and relates to the technical field of medical materials, amino-derived cationic polysaccharide, carboxyl-derived anionic polysaccharide and dopa-modified protein biopolymer materials are used as biological matrix materials, spermidine is used as a bioactive functional component, and the immunoregulation hydrogel is prepared by a one-step method. The preparation method comprises the following steps: by taking a benzaldehyde-terminated high polymer material as a cross-linking agent, forming a first layer of dynamic reversible cross-linked network structure through Schiff base reaction; then calcium ions are introduced, and the calcium ions and carboxylate radicals are complexed and cross-linked to form a second layer of static irreversible cross-linked network structure which can be used for an ESD (Electro-Static Discharge) operation. By adopting a step-by-step cross-linking mode, the first-step cross-linked single-network hydrogel has injectability, self-repairability, tissue adhesiveness and an immune regulation function, and the second-step cross-linking further ensures stable and lasting performance, so that the problems that a submucosal injection material is easy to disperse, short in lasting time and poor in stability are solved. The immunoregulation function effect of the wound repair material is poor.

The invention relates to the technical field of pharmaceutical chemicals, and discloses sequential drug release liposome hydrogel and a preparation method and application thereof, and the preparation method of the liposome hydrogel comprises the following steps: step 1, preparation of drug-loaded positive charge liposome; step 2, preparing phenylboronic acid modified polysaccharide; and step 3, carrying out vortex mixing on the drug-loaded positive charge liposome solution, the phenylboronic acid modified polysaccharide hydrogel precursor powder and the spermidine solution to obtain the liposome hydrogel. According to the sequential drug release liposome hydrogel as well as the preparation method and the application thereof, the liposome hydrogel is prepared by taking high-molecular hyaluronic acid and phenylboronic acid as main raw materials, and the liposome hydrogel has microenvironment responsiveness and time-space characteristics of drug release, and can be used for treating osteoarthritis in an environment with active oxygen increase, so that the drug release effect is good, and the drug release effect is good. The functions of early-stage anti-inflammation and continuous cartilage regeneration are achieved by sequentially releasing double drugs, synovitis can be effectively relieved, cartilage regeneration can be promoted, and the compound can be used for preparing drugs for treating osteoarthritis.

The invention relates to the technical field of spermidine application, in particular to application of spermidine in preparation of medicine for preventing and treating doxorubicin-induced cardiotoxicity and myocardial aging. Cell proliferation experiments and mouse survival rate experiments show that spermidine can reduce death of myocardial cells and mice caused by adriamycin; a q-PCR experiment shows that spermidine can improve abnormal expression conditions of P53, P21, P16, BAX, TNF-alpha, IFN alpha, IL-10 and IL-18 in myocardial cells caused by adriamycin; a beta-galactosidase staining experiment shows that the spermidine can reduce the condition that the expression of a marker beta-galactosidase for myocardial cell senescence induced by adriamycin is increased; a Western Blot experiment shows that the spermidine can improve the condition that the P53 protein is abnormally increased due to the adriamycin; an Elisa experiment shows that spermidine can improve the condition that myocardial injury markers cTn-I and AST and inflammatory factors IL-1beta are abnormally increased due to adriamycin, and the myocardial injury markers cTn-I and AST and inflammatory factors IL-1beta are abnormally increased due to adriamycin. Therefore, spermidine can improve cardiotoxicity and myocardial aging induced by adriamycin.

The invention discloses nucleic acid nano microspheres as well as a preparation method and application thereof, and belongs to the technical field of nano delivery. The preparation method comprises the following steps: carrying out first mixing on nucleic acid and part of water, and carrying out pretreatment and curing to form nucleic acid semi-solid gel; melting the nucleic acid semi-solid gel at 45-80 DEG C, and adjusting the pH value to 4-6 to form a phase A; carrying out second mixing on the spermidine-containing plant extract and the remaining water, and adjusting the pH value to 4-6 to form a phase B; and carrying out third mixing on the phase A, the phase B and an auxiliary agent to form the nucleic acid nano-microsphere, the nucleic acid nano-microsphere is prepared from the following components in percentage by mass: 0.5 to 8 percent of nucleic acid, 0.1 to 7.5 percent of a plant extract containing spermidine, 0 to 6 percent of an auxiliary agent and 78.5 to 99.4 percent of water, the mass ratio of the nucleic acid to the plant extract containing spermidine is (1-10): 1. The nucleic acid nano-microsphere has improved transdermal effect and stability, and can be used for transdermal drug delivery.

The invention relates to a method for extracting spermidine from a natural source. The method comprises the following steps: (1) extracting a natural source substance to obtain a crude extract; and (2) carrying out hydrophilic interaction liquid chromatography purification on the crude extract to obtain spermidine. According to the method disclosed by the invention, the extract from a natural source is efficiently and accurately separated by utilizing the hydrophilic interaction liquid chromatography, so that the high-purity spermidine with a wide source is obtained to meet the requirement on high-quality spermidine.

The invention relates to the technical field of biological detection, and discloses a mycoplasma pneumoniae nucleic acid rapid detection method, which comprises the following steps: mixing a sample to be detected with a multifunctional pretreatment buffer solution containing tris (hydroxymethyl) aminomethane, potassium acetate, polyethylene glycol, saponin, spermine tetrahydrochloride and tris (2-carboxyethyl) phosphine hydrochloride, and carrying out cracking treatment; a cracking mixed solution is obtained; redissolving the enzymatic isothermal amplification basic reagent, the specific primer and the probe to obtain a redissolving amplification system; sucking the supernatant of the cracking mixed solution, adding the supernatant into a redissolving amplification system, and adding a magnesium acetate solution to obtain a reaction mixed solution to be detected; and carrying out isothermal amplification and signal acquisition to obtain a detection conclusion. The multifunctional pretreatment buffer solution is used for cracking the to-be-detected sample, sample liquefaction, cracking and nucleic acid release are completed in a single system, the nucleic acid extraction and purification step of centrifugal column adsorption or magnetic bead elution is avoided, and the operation process is simplified.

A nutraceutical composition, consisting of Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.

The invention discloses application of spermidine in preparation of a medicine for relieving copper exposure injury, and relates to the field of biological medicine. The invention provides an application of spermidine in preparation of a medicine for relieving copper exposure injury, and the injury is liver injury and / or intestinal injury caused by copper exposure. Spermidine can reduce the copper content in serum and liver, promote the expression of liver copper transporters ATP7B and CTR1, copper death proteins FDX1, LIAS and Lip-DLAT, and reduce the oligomerization of HSP70 and DLAT proteins; meanwhile, transaminase activity in serum and liver and intestinal tissue damage are reduced. The medicine provided by the invention can improve intestinal health, improve liver copper metabolism capability and relieve body injury caused by copper exposure, effectively solves the problem that no reliable medicine exists for related injury caused by copper exposure, and is expected to be developed into a medicine or health food for preventing and treating copper exposure injury.

The invention discloses a method for preparing spermidine. The method comprises the following steps: dissolving oxalyl chloride and a base in a first solvent, slowly adding 4-bromo-1-butylamine hydrobromic acid, and reacting to obtain 2-((4-bromobutyl)amino)-2-oxoacetyl chloride; adding a base to the reaction solvent of the obtained 2-((4-bromobutyl)amino)-2-oxoacetyl chloride, and slowly adding 3-bromo-1-propylamine hydrobromic acid to obtain N 1 ‑(4‑Bromobutyl)‑N 2 ‑(3‑bromopropyl)oxalamide; 1 ‑(4‑Bromobutyl)‑N 2 ‑(3‑bromopropyl)oxalamide, benzylamine and a base are added to a second solvent to react to obtain 8‑benzyl‑1,4,8‑triazacyclododecane‑2,3‑dione; 8‑benzyl‑1,4,8‑triazacyclododecane‑2,3‑dione is added to an acid for hydrolysis to obtain N 1 ‑(3‑Aminopropyl)‑N 1 Benzylbutane-1,4-diamine; N 1 ‑(3‑Aminopropyl)‑N 1 Benzylbutane-1,4-diamine is added to Raney nickel / methanol, and hydrogen is passed through the reaction to obtain crude spermidine, which is then distilled to obtain pure spermidine. The preparation method of the present invention is easy to operate and safe, easy to industrial-scale production, and has a high conversion rate of spermidine and high product purity.

The invention belongs to the technical field of biology, and particularly discloses application of laccase AmLac in degradation of biogenic amines. The amino acid sequence of the laccase is shown as SEQ ID NO.1. The laccase has different degrees of degradation effects on the biogenic amines such as histamine, spermidine, tyramine, phenylethylamine and putrescine, and the degradation rates of the histamine and the putrescine both reach 100%. The enzyme is stable in enzymatic property, has alcohol tolerance and acid tolerance, has certain adaptive capacity to food matrixes, and can effectively degrade biogenic amines in wine and reduce potential hazards of the biogenic amines to human bodies, so that the safety and stability of products are improved. Therefore, the AmLac has a wide application prospect in the food industry, and especially when the AmLac is used for treating food containing high biogenic amine, the safety quality of the food can be remarkably improved.

The present invention relates to the technical field of anti-aging compositions and particularly to an anti-aging composition and use thereof, and an anti-aging capsule and use thereof. The present invention provides an anti-aging composition comprising the following components: 20-2000 parts by mass of calcium α-ketoglutarate, 20-100 parts by mass of quercetin, 20-2000 parts by mass of trans-resveratrol, 20-100 parts by mass of calendulin, and 0.02-1.8 parts by mass of spermidine. The composition of the present invention has a multi-target synergistic effect on 12 major causes of aging and can safely and effectively reverse aging, enhance the physiological function, reduce the risk of chronic diseases, and improve the quality of life. The components of the composition are safe, and are carefully selected natural food ingredients; therefore, the composition is safe and reliable, and has no side effects with long-term use. The composition is prepared in an oral dosage form, so that the composition is convenient to administer, which improves patient compliance; moreover, the mode of application of the composition can be expanded.

A method of improving and / or enhancing at least one of bone mineralization, bone strength, bone mass, and bone mineral density in an individual in need thereof, the method comprising administering to the individual a composition comprising one or more anabolic amino acids, and the composition further comprising one or more autophagy-inducing compounds. In one embodiment, the one or more autophagy-inducing compounds are selected from the group consisting of thymol, carvacrol, spermidine, urolithin (e.g., Urolithin A, B or D), rapamycin, Torin1, valproic acid, polyphenols (e.g., resveratrol), caffeine, metformin, 5′ AMP-activated protein kinase (AMPK) activators, L-type calcium channel inhibitors, ketones (e.g. beta-hydroxybutyrate, ketone salts, or ketone ester derivatives), 4,4′-dimethoxychalcone and mixtures thereof.

The invention discloses application of spermidine in preparation of an agent for resisting mitochondrial oxidative damage and copper death damage, and relates to the technical field of biology. The invention provides an application of spermidine in preparing an agent for resisting mitochondrial oxidative damage and copper death damage, and further provides an application of spermidine in inhibiting copper death induced by copper sulfate and relieving iron-sulfur cluster protein loss and ester acylation protein aggregation induced by copper accumulation. The spermidine can obviously relieve cell viability decline induced by copper sulfate, reverse ROS and MDA level increase and mitochondrial membrane potential decline induced by copper sulfate and relieve iron-sulfur cluster protein loss and ester acylation protein aggregation induced by copper accumulation, and has obvious effects of inhibiting mitochondrial oxidative damage and copper death. The invention provides a method for effectively relieving copper death of ovarian granular cells, and solves the problem that the existing antioxidant and cytoprotective agent for resisting mitochondrial oxidative damage generate side effects in the use process.

The invention belongs to the technical field of natural medicines, and particularly relates to application of spermidine alkaloid in preparation of anti-tumor medicines and / or reversion of drug resistance. The invention provides an application of macrocyclic spermidine alkaloid in preparation of anti-tumor drugs and / or reverse drug resistance. The macrocyclic spermidine alkaloid comprises one or more than two of 13-oxo-furan celastrus orbiculatus, furan celastrus orbiculatus, benzene-substituted celastrus orbiculatus and celastrus orbiculatus cinnamamide alkali. The macrocyclic spermidine alkaloid disclosed by the invention has the characteristics of broad-spectrum anti-tumor effect, good anti-tumor effect, relatively low toxic and side effects, capability of reversing 5-fluorouracil drug resistance, relatively high water solubility, relatively easy source, simple structure, only one chiral carbon in the structure, relatively easy artificial synthesis, intervention in cell autophagy mechanism and the like, so that the overall treatment cost is relatively low; the method has a potential important clinical application prospect.

The invention discloses a fluorescent probe for detecting spermine and spermidine in a water sample and urine as well as a preparation method and application of the fluorescent probe, and relates to the technical field of supramolecular fluorescent probes, and the fluorescent probe is formed by reacting 14-membered cucurbit urils (tQ [14]) and a compound (E)-1-(4-boron benzyl)-4-(4-hydroxyl styryl) quinoline-1-ammonium bromide according to a molar ratio of 1: 1. The novel fluorescent probe provided by the invention can specifically detect spermine and spermidine in a water sample and urine, and the preparation method of the fluorescent probe is simple and easy to implement; the method for detecting spermine and spermidine in a water sample and urine by using the fluorescent probe has the advantages of high sensitivity, high selectivity, low cost, simple sample treatment, convenient operation, rapid determination and the like.

The invention relates to the field of oral cavities, in particular to a biomarker for drug-related jawbone necrosis and application of the biomarker. The invention provides application of a biomarker to preparation of a drug-related jawbone necrosis detection product. The biomarker comprises an oral metabolite and an oral flora, the oral metabolite comprises one or a combination of more of spermine, 7-methylguanine, hypoxanthine, quinoline and taurine. And the oral flora comprises bacteria of bacteroides, peptostreptococcaceae, porphyrinomonas, butyric acid vibrio, micromonas and moniliaceae, and the oral flora comprises bacteria of bacteroides, peptostreptococcaceae, porphyrinomonas, butyric acid vibrio. According to the application, metabolite and microorganisms which are characteristic-changed in an MRONJ microenvironment are discovered, the pathogenesis that the metabolite and the microorganisms possibly participate in the MRONJ is predicted, the risk that an individual suffers from the MRONJ is expected to be judged in an early stage through oral metabolite and microorganism marker screening, occurrence of the MRONJ is prevented by adjusting a medication strategy or modifying oral flora in time, and the MRONJ is prevented from being damaged by the oral metabolite and the microorganism. Therefore, the method is of great significance to formulating a new osteoporosis clinical prevention and treatment strategy.

The invention provides a paclitaxel molecular umbrella delivery system prodrug as well as a preparation method and application thereof. The paclitaxel molecular umbrella delivery system prodrug comprises a molecular umbrella target head part which is formed by connecting two surface amphiphilic molecules through spermidine; a paclitaxel drug moiety; and a linking group that links the molecular umbrella target head portion to the paclitaxel drug portion through a disulfide bond; wherein the surface amphiphilic molecule is selected from cholic acid, deoxycholic acid or sodium sulfonate thereof. Compared with paclitaxel, the paclitaxel molecular umbrella delivery system prodrug has the advantages that the water solubility is obviously improved, the toxicity to normal cells is lower than that of free paclitaxel, and the paclitaxel molecular umbrella delivery system prodrug has obviously improved safety; the paclitaxel molecular umbrella delivery system prodrug has the following characteristics in a physiological environment: an active drug is released in a high GSH / ROS microenvironment of tumor cells; and maintaining the prodrug state in normal cells.

The present invention relates to a composition for modulating the release of biologically active aliphatic polyamines, said composition comprising palmitamide ethanol and preferably a sucrose ester as an agent for modulating the release of aliphatic polyamines from the composition. The composition may be used directly or added to a formulation of a supplement to promote hair growth.

The invention discloses a preparation method of an edible flower phenol amide compound and application of the edible flower phenol amide compound in prevention and treatment of non-alcoholic fatty liver disease, and particularly relates to the field of development of new application of food raw materials. The edible flower phenol amide compound N1, N5, N10-coumaroyl spermidine (TCS) can be green extracted by using a natural deep-eutectic solvent (NADES), and can inhibit free fatty acid-induced liver cell lipid accumulation and improve fatty acid-caused liver cell fatty degeneration; oxidative stress and inflammation induced by free fatty acid are inhibited, and expression of senescence-related markers is inhibited, so that development of NAFLD is effectively inhibited.

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

The invention relates to the technical field of forest cultivation, and discloses a carya illinoensis fruit forest variety configuration method which comprises the following steps: configuring a main cultivation variety and a pollination variety in a fruit forest; preparing a pollination synergistic composition; the composition is sprayed at the initial stage of stigma exposure of female flowers of a main cultivated variety. The composition comprises a phenolic acid grafted carboxymethyl chitosan derivative, low methoxyl pectin, a calcium ion cross-linking agent, sugar alcohol chelated boron and spermidine. According to the method, an interfacial film with antioxidant activity is formed on the surface of the stigma by using the grafting modified matrix, so that the stigma browning is effectively inhibited, and the pollination period is prolonged; by constructing a calcium cross-linked low-methoxyl pectin interpenetrating network, an interfacial film is endowed with an intelligent degradation characteristic of enzyme-sensitive response to pollen, and protection at ordinary times and immediate dissolution after touching flowers are realized. According to the method, pollen is captured through static electricity, nutritional factors are released in a targeted mode, the pollination and fertilization efficiency is remarkably improved, the sterility risk in the flowering phase is relieved, and the yield and quality of carya illinoensis are improved.

The application discloses a method for detecting plant free polyamines by HPLC, which can clearly separate the target substances putrescine (Put), spermidine (Spd), thermospermine (T-Spm) and spermine (Spm) in a plant sample. The method has stable retention time of the separated target substances, and the peaks corresponding to the target substances separated from different samples and the standard sample can be well overlapped, data is clear, and the result is reliable. The separation time is shortened by half, the use amount of the mobile phase is halved, the operation cost is greatly reduced, the waste liquid is halved, the recovery cost of the waste liquid is also halved, and the potential pollution to the environment is reduced.

The invention discloses application of spermidine in in-vitro culture of human unfrozen oocytes and embryos. By adding the combination of spermidine, melatonin and astaxanthin into the thawed oocyte resuscitation culture solution and the embryo in-vitro culture solution, the mitochondrial autophagy and mitochondrial functions of the thawed oocytes are improved, so that the in-vitro culture effect of the thawed oocytes and embryos is improved, and the success rate of an assisted reproduction technology is increased.

The invention discloses an SERS (Surface Enhanced Raman Scattering) flexible sensor based on dual-ion modification as well as a preparation method and application of the SERS flexible sensor, and belongs to the technical field of biomedical detection. A bacterial cellulose membrane (BCM) is used as a base material, cation modification (QBCM) is carried out through glycidyl trimethyl ammonium chloride (EPTMAC), anion modification (TABCM) is carried out through tannic acid (TA), a functional substrate with asymmetric charge distribution and a porous structure is constructed, then Ag NPs are loaded on the modified base material through an in-situ reduction method, the QBCM-coated AgNPs and TABCM-coated AgNPs dual-ion active substrate is formed, and the AgNPs / TABCM-coated AgNPs dual-ion active substrate is used for preparing the composite material. The SERS flexible sensor is integrated through a layer-by-layer self-assembly (LbL) technology; the SERS flexible sensor can realize specific enrichment and synchronous detection of anionic kynurenic acid (KYNA) and cationic spermine (Spm) in urine, and is low in detection limit and good in reproducibility.

The invention discloses application of spermidine in preparation of a preparation for improving fertility of female mammals, and belongs to the technical field of reproductive biology. According to the invention, spermidine with different concentrations is introduced into mice, and the effect of spermidine on ovarian hormone secretion, ovarian reserve level and follicle number of female mammals is determined in a diet intake manner, so that the fertility of female animals is improved; it is found that spermidine can effectively improve the ovarian function, increase the secretion level of E2, P4, LH and AMH hormones closely related to the ovarian function, reduce the number of atresia follicles of the ovarian and increase the litter birth number of female mice by 1-2, and the spermidine has good animal breeding and livestock raising application prospects.

The present invention provides a method for preparing spermidine, which relates to the technical field of organic synthesis. The method for preparing spermidine comprises: condensing 3-chloropropylamine hydrochloride and triphenylmethane to obtain 3-chloro-N-tritylpropylamine; condensing 3-chloro-N-tritylpropylamine and 1,4-butanediamine to obtain N 1 ‑(3‑(tritylamino)propyl)butane‑1,4‑diamine; N 1 The trityl group is removed from 1,4-(3-(tritylamino)propyl)butane to obtain spermidine hydrochloride; and spermidine hydrochloride is liberated in the presence of a base to obtain spermidine. The preparation method of the present invention can achieve a high yield and purity in a relatively short reaction period, avoids the problems of a high proportion of by-products, high purification difficulty, and residual solvent, improves the reaction efficiency, and has mild reaction conditions, simple production equipment, and is more energy-saving and environmentally friendly while being convenient for industrialized amplification production.

The invention discloses a composition for promoting bulb expansion of hippeastrum hippeastrum, the composition is an aqueous solution, the solute in the composition comprises putrescine, spermidine, spermine, triacontanol, ethanol, Tween 80, sodium lignin sulfonate and ethylene glycol, and the dosage ratio of the materials is 10: 1: 2: 0.05: 10: 5: 3: 2 according to the dosage weight. And diluting the composition with water to obtain a diluent with the mass percent concentration of 0.04-0.06%. The diluent is used for foliage spraying and seedball base irrigation in the germination stage, the growth stage, the flowering stage and the post-flowering rejuvenation stage of hippeastrum hippeastrum, the seedball diameter of hippeastrum hippeastrum can be remarkably increased, and economic benefits are increased.

The invention provides an in-vitro transcription method based on RNA polymerase and a kit, which are suitable for the fields of mRNA synthesis, gene research and biological pharmacy. According to the method, the problems of low efficiency, poor stability and the like of traditional in-vitro transcription are solved through an optimization technology. The method comprises the following specific steps: providing a linear DNA template containing a T7 promoter; according to the method, site-directed mutagenesis or fusion protein modified high-activity T7RNA polymerase is combined with chemically modified NTPs and a spermine-BSA compound stabilizer to react under the conditions that the temperature is 37-42 DEG C and the pH is 7.5-8.5, and formation of double-stranded RNA is promoted through dynamic temperature control. A reaction system contains Mg < 2 + > / Mn < 2 + > and DTT, and enzyme activity and product integrity are optimized. The kit comprises an improved reagent and a standardized process, the transcription efficiency is improved by 2-5 times, the product yield is greater than or equal to 80%, and the activity is not lost after the kit is stored at 4 DEG C for 1 month. The method has the technical advantages that NTPs degradation resistance is achieved, template preparation is simplified, universality is high, the method is suitable for large-scale production of mRNA vaccines, gene therapy and the like, the production cost is remarkably reduced, and wide application of the in-vitro transcription technology is promoted.

The invention belongs to the technical field of food additives, and particularly relates to a nutrition enhancer as well as a preparation method and application thereof. The nutrition enhancer comprises a component I derived from plant polypeptide, a component II derived from iron, a component III derived from calcium, a component IV derived from zinc and an absorption enhancer, the absorption enhancer is composed of spermine and rhizoma atractylodis polysaccharide, wherein the spermine is loaded by silicon dioxide microspheres coated with modified chitosan. Under the action of the absorption enhancer, the nutrition enhancer disclosed by the invention can promote effective absorption of calcium, iron and zinc, and meanwhile, the content of serum antibodies can be increased.

This invention discloses a kit and method for extracting cell nuclei from plant tissues, belonging to the field of biotechnology. The kit includes a nuclear lysis buffer and a nuclear washing buffer. The nuclear lysis buffer is prepared using nuclease-free water with sucrose, Tris-HCl (pH 7-8), NaCl, MgCl2, a surfactant, DTT, an RNase inhibitor, and spermidine. The nuclear washing buffer is prepared using nuclease-free water with sucrose, Tris-HCl (pH 7-8), NaCl, MgCl2, DTT, an RNase inhibitor, and spermidine. This kit is suitable for extracting cell nuclei from fresh yew stem tissue, yielding cell nuclei with intact nuclear membranes, stable gene expression, high purity, and few impurities, suitable for single-cell sequencing.